Process for fermenting industrial hemp waste by using trichoderma complex microbial inoculant

Abstract

The invention discloses a process for fermenting industrial hemp waste by using a trichoderma complex microbial inoculant, which comprises the following steps: S1, raw material preparation: preparing the following components in parts by weight: 2-4 parts of complex zymophyte, 2-4 parts of bacillus subtilis, 98-196 parts of industrial hemp waste mixture, 2-4 parts of common calcium superphosphate and 0.1-0.2 part of regulator; 50 to 100 parts of water; s2, putting the prepared raw materials into a fermentation tank, and uniformly mixing to obtain a mixture; s3, placing the mixture in a fermentation tank for aerobic fermentation, monitoring the fermentation temperature, and turning when the fermentation temperature rises to 50-55 DEG C; and S4, continuously fermenting for 10-15 days, and sufficiently fermenting and decomposing the industrial hemp waste and the like, so as to obtain a fermented product which is used as a raw material in production of an organic fertilizer, a biological organic fertilizer and an organic-inorganic compound fertilizer. The industrial hemp waste can be fully fermented and decomposed to serve as a raw material in production of organic fertilizer, bio-organic fertilizer and organic-inorganic compound fertilizer; the purpose of producing the fertilizer by utilizing the waste is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of fermentation treatment, and in particular to a process for fermenting industrial hemp waste by using a composite fungus agent of Trichoderma. Background Art

[0002] Industrial hemp waste has a high cellulose content and a carbon-nitrogen ratio of 34:1. It is not easy to completely ferment it using ordinary fermentation bacteria, and secondary fermentation is prone to occur during organic fertilizer processing.

[0003] Therefore, in order to solve the above problems, this paper proposes a process for fermenting industrial hemp waste using a composite fungus of Trichoderma, which can increase the decomposition rate of cellulose and fully ferment the waste. Summary of the invention

[0004] The purpose of the present invention is to fully ferment and decompose industrial hemp waste and use it as a production ingredient for organic fertilizer, bio-organic fertilizer, and organic-inorganic compound fertilizer; to achieve a process for fermenting industrial hemp waste using a Trichoderma composite fungus agent to produce organic fertilizer from waste.

[0005] In order to achieve the above technical effects, the present invention is implemented by the following technical scheme: a process for fermenting industrial hemp waste using a composite fungus agent of Trichoderma, characterized in that it includes the following steps:

[0006] S1. Raw material preparation: by weight, the components are: 2-4 parts of composite fermentation bacteria, 2-4 parts of Bacillus subtilis, 98-196 parts of industrial hemp waste mixture, 2-4 parts of ordinary superphosphate, 0.1-0.2 parts of regulator; 50-100 parts of water;

[0007] S2, placing the prepared raw materials in a fermentation tank, mixing them evenly, and obtaining a mixture;

[0008] S3, placing the mixed material in a fermentation tank for aerobic fermentation, monitoring the fermentation temperature, and turning the compost when the fermentation temperature rises to 50-55°C;

[0009] S4. Continue fermenting for 10 to 15 days to fully ferment and decompose the industrial hemp waste, thereby obtaining a fermented product that can be used as a raw material in the production of organic fertilizer, bio-organic fertilizer, and organic-inorganic compound fertilizer.

[0010] Furthermore, the composite fermentation bacteria is prepared by mixing 40% of Trichoderma, 20% of a first mixed bacteria prepared from cellulose-degrading composite bacteria and actinomycetes, and 40% of a second mixed bacteria prepared from methylotrophic Bacillus, Bacillus licheniformis, Bacillus amyloliquefaciens and Bacillus subtilis.

[0011] Furthermore, the spore content in the composite fermentation bacterial agent is not less than 200 million / g.

[0012] Furthermore, the moisture content in the industrial hemp waste is less than 10%.

[0013] Furthermore, the fermentation temperature is monitored by inserting a thermometer into the mixture in the fermentation tank.

[0014] Furthermore, the industrial hemp waste mixture is prepared by mixing straw charcoal, sheep manure, orange stalk powder, biogas residue, wood ash and hemp waste debris in a mass ratio of 2-4:2-4:0.5-1:0.2-0.4:0.3-0.6:3-6.

[0015] Furthermore, the industrial hemp waste debris is the debris made by crushing the roots, stems, leaves and seed shells left after planting and stripping hemp fibers, collecting seeds and pressing oil; industrial hemp refers to hemp with a tetrahydrocannabinol content of less than 0.3%.

[0016] Furthermore, the water content of the biogas residue is 45%-60%.

[0017] Furthermore, the regulator is formed by mixing borax, zinc sulfate and ferrous sulfate in a mass ratio of 1-2:1-2:1-2.

[0018] The beneficial effects of the present invention are:

[0019] The process of the present invention shortens the fermentation process of industrial hemp waste to 10 days, which is 5 days shorter than the 15 days required for ordinary fermentation, or 30% shorter; the number of compost turning times is reduced by 1 time, or 33%, compared with 3 times required for ordinary fermentation; in the fermentation process, the labor required for ordinary fermentation is reduced by 10%; in the fermentation process, 15% less compost turning equipment is used than that required for ordinary fermentation; the turnover utilization rate of the fermentation tank is increased by 20%; and the production speed and output are accelerated. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0021] Figure 1 It is the overall flow chart of the process of the present invention. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] This example describes the specific process of fermenting industrial hemp waste using a Trichoderma composite inoculant, as follows:

[0025] 1. Cultivation of compound fermentation strains;

[0026] The composite fermentation microbial community used does not contain any pollutants and chemical hazards. It can utilize the characteristics that the beneficial substances produced by the microbial communities during their respective growth processes can serve as growth substrates for each other and form a symbiotic proliferation relationship. By selecting appropriate flora structures and adopting unique fermentation processes, a composite microbial water quality improvement preparation with complex flora, complete functions and stable structure is finally formed; it can quickly decompose cellulose, citrus stalks, starch, protein, amino nitrogen, nitrite nitrogen, and eliminate harmful substances such as hydrogen sulfide;

[0027] 2. Cultivation methods:

[0028] Put water into a plastic bucket → add brown sugar → add compound fermentation bacteria → dissolve urea and potassium dihydrogen phosphate respectively and add → shake well → seal → cultivate under light, keep the temperature at 25-35 degrees → shake twice a day, ventilate for half an hour each time → add D-isoascorbic acid on the third day, shake well → stop light on the fourth day, test the pH value, and cultivate if it reaches below 3.8, continue to cultivate if it does not → open the lid for ventilation, cultivate after three days, seal for storage or use; if the temperature is low and the light is insufficient, the cultivation time will definitely be extended, and it may also cause failure, and the use effect will also be affected; whether the cultivation is completed is determined by the pH test paper. If the pH value is above 3.8, it is qualified;

[0029] The cultivated composite fermentation bacteria should be sealed and kept away from light (not exposed to direct sunlight) and can be kept for one year; the successfully cultivated composite fermentation bacteria can be used for inoculation within 3 months to continue to expand the cultivation. It is not recommended to continue to use the bacteria that are more than 3 months old as seeds; use effective microbial flora (diluted 500 times), mix it into the fermentation materials, and ferment together.

[0030] 3. Make dilution solution:

[0031] Use one part of compound fermentation bacteria and one part of brown sugar (weight 1:1), first dissolve the brown sugar with hot water, then add the required multiple of water to make a dilution, and use it immediately after preparation. For example, adding 500 times of water will make a 500-fold dilution;

[0032] 4. Make fermentation material:

[0033] Use one part of compound fermentation bacteria and one part of brown sugar (weight 1:1), first use hot water to dissolve the brown sugar, wait for it to cool, pour in the compound fermentation bacteria, add water to mix, then add the amount of material to be fermented (feed, feces or mixed material) and mix well into powder. The moisture should be such that it can be kneaded into a ball by hand and it will fall apart when touched. Then put it into a sealed container and store it for 4-10 days for fermentation. When the material emits a strong aroma of slightly sweet and sour wine, it indicates that the fermentation is successful.

[0034] The nutrient determination of organic fertilizer raw materials, the indicators are shown in Table 1;

[0035]

[0036] Table 1

[0037] The organic fertilizer processing method using hemp waste fermentation products as raw materials comprises the following steps: using biogas residue (water content 45%-60%), grass carbon, sheep manure, orange stalk powder, biogas residue, plant ash and hemp waste debris in a mass ratio of 2:2:0.5:0.2:0.3:3 to mix into a hemp waste mixture, adding 10% ordinary superphosphate in a mass ratio of hemp waste mixture: ordinary superphosphate = 98:2, then adding 0.2% composite fermentation bacterial liquid and 0.2% Bacillus subtilis liquid in a mass ratio of hemp waste mixture: composite fermentation bacterial liquid: Bacillus subtilis = 98:2:2, and then adding a regulator in a mass ratio of hemp waste mixture: regulator = 98:0.1; mixing well, turning and fermenting in a fermentation tank according to the fermentation process of organic fertilizer, reaching the standard specified in the ordinary organic fertilizer "NY525--2012", the indicators are not repeated, and it is used as a carrier auxiliary material for standby;

[0038] 1. Overview of production process:

[0039] Organic fertilizer products are mainly made of hemp waste, sheep manure, and orange stalk powder. They are added with composite fermentation bacteria and Bacillus subtilis for fermentation treatment. Qualified organic fertilizer products that meet (NY525-2012) are produced through crushing, mixing, screening, inspection, and packaging.

[0040] The formula of the organic fertilizer prepared by the process of fermenting industrial hemp waste using a composite fungus agent of Trichoderma is shown in Table 2 below:

[0041]

[0042]

[0043] Table 2

[0044] The product effect is: this product technical solution utilizes the characteristics of biological functional bacteria (Bacillus) in organic fertilizer to use organic matter as nutrients and reproduce itself, and makes biological composite bacteria organic fertilizer. When applied to the soil, the biological bacteria can reproduce itself. In the rhizosphere soil of plants, Bacillus and fermented bacteria use organic matter as nutrients to reproduce in large quantities and establish dominant bacterial communities; Bacillus and fermented bacteria communities produce a large amount of resistance substances to control the reproduction of plant root-knot nematodes, thereby achieving the purpose of controlling plant root-knot nematodes; this product technical solution uses organic fertilizer and biological organic fertilizer of Bacillus and fermented bacteria to expand the inhibitory spectrum, expand the control surface, and provide organic matter for the soil; the Bacillus and fermented bacteria agents in this product technical solution are all commercially available products, and the preparation method is simple and easy to produce. Organic fertilizer raw materials are widely available and low in cost. In the technical solution of this product, organic fertilizer produced by fermentation of hemp waste as raw materials increases the organic matter in the soil where plants grow. Organic matter can serve as nutrients for Bacillus and fermentation bacteria, and can promote the reproduction of Bacillus and fermentation bacteria and the establishment of dominant soil flora. At the same time, the decomposition of organic matter by Bacillus can also decompose macromolecules that are difficult to be absorbed by plant roots into small molecules that are easy to absorb, promote the growth of plants, and improve the effect of fermentation bacteria in controlling nematodes, so plants, flora and soil can promote each other. At the same time, the use of biological methods significantly reduces environmental pollution compared to chemical control methods. This method is low in cost, easy to promote, and has good application prospects.

[0045] Example 2

[0046] In this example, the fertilizer prepared in Example 1 was specifically applied to farmland to verify the effect, as follows:

[0047] Field test results:

[0048] Comparative test results of citrus yield in the same region

[0049] The organic fertilizer produced using fermented hemp waste as raw material is a kind of organic fertilizer with multiple beneficial microorganisms, which has good growth-promoting and disease-resistant effects on crops, and enhances the stress resistance of crops, thereby reducing the use of chemical pesticides, and has good beneficial microbial active preparations for the environment. Through field trials, the effect of "citrus-specific organic fertilizer" in field application has been verified, and it is intended to be used in production to achieve the purpose of preventing diseases and increasing income.

[0050] Result analysis:

[0051] The organic fertilizer has an 11.5% yield increase effect on sugar oranges. It enhances the disease resistance of the plants, increases the rate of high-quality fruits by 8%, and increases the solid content by 1.3%. The organic fertilizer has a growth-promoting effect on citrus fruits; the appearance, sweetness, and palatability have been improved. In production, at least 5 kg of fertilizer should be used per plant in combination with chemical fertilizers as winter fertilizer.

[0052] The application demonstration results in citrus production areas,

[0053] The demonstration results in multiple citrus producing areas in Yunnan show that the organic fertilizer produced using fermented hemp waste as raw materials has been applied to more than 30,000 acres and 15,000 tons from 2020 to 2023, which has a significant value-added effect on citrus and has good promotion and application value.

Claims

1. A process for fermenting industrial hemp waste using a composite fungus agent of Trichoderma, characterized in that: The steps include: S1. Raw material preparation: by weight, the components are: 2-4 parts of composite fermentation bacteria, 2-4 parts of Bacillus subtilis, 98-196 parts of industrial hemp waste mixture, 2-4 parts of ordinary superphosphate, 0.1-0.2 parts of regulator; 50-100 parts of water; S2, placing the prepared raw materials in a fermentation tank, mixing them evenly, and obtaining a mixture; S3, placing the mixed material in a fermentation tank for aerobic fermentation, monitoring the fermentation temperature, and turning the compost when the fermentation temperature rises to 50-55°C; S4. Continue fermenting for 10 to 15 days to fully ferment and decompose the industrial hemp waste, thereby obtaining a fermented product that can be used as a raw material in the production of organic fertilizer, bio-organic fertilizer, and organic-inorganic compound fertilizer.

2. A process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The composite fermentation bacteria is prepared by mixing 40% of Trichoderma, 20% of a first mixed bacteria prepared from cellulose-degrading composite bacteria and actinomycetes, and 40% of a second mixed bacteria prepared from methylotrophic Bacillus, Bacillus licheniformis, Bacillus amyloliquefaciens and Bacillus subtilis.

3. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The spore content in the composite fermentation bacterial agent is not less than 200 million / g.

4. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The moisture content in the industrial hemp waste is less than 10%.

5. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The fermentation temperature is monitored by inserting a thermometer into the mixed material in the fermentation tank.

6. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The industrial hemp waste mixture is prepared by mixing grass carbon, sheep manure, orange stalk powder, biogas residue, wood ash and hemp waste debris in a mass ratio of 2-4:2-4:0.5-1:0.2-0.4:0.3-0.6:3-6.

7. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The industrial hemp waste debris is the debris made by crushing the roots, stems, leaves and seed shells left after planting and stripping hemp fibers, collecting seeds and pressing oil; industrial hemp refers to hemp with a tetrahydrocannabinol content of less than 0.3%.

8. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 7, characterized in that: The water content of the biogas residue is 45%-60%.

9. The process for fermenting industrial hemp waste using a composite Trichoderma inoculum according to claim 1, characterized in that: The regulator is prepared by mixing borax, zinc sulfate and ferrous sulfate in a mass ratio of 1-2:1-2:1-2.

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