Nutritional composition containing deeply hydrolyzed casein and food and application thereof

Abstract

The invention discloses a nutritional composition containing deeply hydrolyzed casein as well as food and application of the nutritional composition. The nutritional composition comprises the deeply hydrolyzed casein, solid corn syrup, lactose-N-neotetraose, 2 '-fucosyllactose and lactobacillus rhamnosus GG (Lactobacillus rhamnosus GG), and the food is prepared from the deeply hydrolyzed casein, the solid corn syrup, the lactose-N-neotetraose, the 2'-fucosyllactose and the lactobacillus rhamnosus GG. The invention also relates to a formula food containing the nutritional composition. The nutritional composition or the formula thereof can improve infant food protein allergy and / or gastrointestinal dysfunction.

Description

Technical Field

[0001] The present application belongs to the field of food technology, and relates to a nutritional composition containing extensively hydrolyzed casein for infant or toddler formula food, and food and uses thereof, wherein the composition can improve infant food protein allergy and / or gastrointestinal dysfunction. Background Art

[0002] Food protein allergy is an adverse reaction caused by an inappropriate immune response to protein in food in infants. At the same time, allergy to food proteins such as milk and soy is one of the common causes of gastrointestinal dysfunction symptoms. The prevalence of 0-3-year-old infants is between 2% and 7.5%. Food protein allergy may cause eczema, vomiting, diarrhea and even shock in infants, resulting in growth disorders and nutrient deficiencies.

[0003] Infants and young children of families with a history of food protein allergy are more likely to have allergic reactions between the ages of 0 and 7, and this problem cannot be avoided by breastfeeding. The evidence-based recommendations for the diagnosis and treatment of milk protein allergy in infants and young children in China mentioned: If the symptoms of the child reappear after the mother avoids milk and its products, the mother should avoid the diet during breastfeeding and give a deep hydrolyzed protein formula or amino acid formula replacement after weaning from breast milk. DRACMA (Guidelines on the Diagnosis and Related Theories of Milk Protein Allergy) (2010) pointed out that deep hydrolyzed formulas can be used for functional gastrointestinal diseases and allergy-induced gastroenteritis and proctitis. Enteral nutrition for critically ill patients with gastrointestinal dysfunction. The expert consensus on enteral nutrition for critically ill patients with gastrointestinal dysfunction (2021 edition) mentioned: Since intestinal epithelial cells absorb short peptides, hydrolyzed protein nutritional formulas can be used for critically ill patients with whole protein intolerance; patients with gastrointestinal dysfunction such as short bowel syndrome can consider giving short peptide pre-digested formulas; intestinal microbiome regulation is achieved through changes in diet, taking prebiotics, probiotics, synbiotics, and fecal microbiome transplantation. The expert consensus on perioperative nutritional management of children (2019) mentioned that if the child's digestive and absorption function is impaired after surgery, a deeply hydrolyzed formula can be used instead.

[0004] M.J. Kushilis et al. improved infant protein allergy by deep hydrolyzing whey protein, which stipulated that the peptide molecular weight of hydrolyzed whey protein was not detected if it was greater than 3000 Da, about 99% if it was less than 1500 Da, about 98% if it was less than 1200 Da, and about 52% if it was 240-600 Da. This segmentation is more complicated, and the segmentation requirements are not confirmed for the allergenic mechanism. At the same time, the whey protein raw material itself contains more allergenic residues; after the protein is hydrolyzed, there is a risk of deficiency of some essential amino acids, which may cause malnutrition in infants.

[0005] Protein itself has bitterness and peculiar smell after hydrolysis, and the dissolving characteristics of infant formula powder are particularly important. He Guanghua et al. used fresh milk, desalted whey powder, and whey peptides with a molecular weight of less than 10,000 Da as protein sources in infant formula milk powder in Chinese patent application CN101978841A to form an anti-protein allergy and nutritious infant formula milk powder, in which the difference in the amino acid composition and content of the peptides may cause the final infant formula milk powder to taste bitter.

[0006] Xu Qingli et al. mentioned the beneficial effects of each single nutrient on allergic infants in Chinese patent application CN114176219A, but did not explore whether there is a synergistic effect between the nutrients in terms of function; at the same time, they did not consider the impact of each raw material on the preparation and taste of the final product.

[0007] In summary, existing food protein allergy-related compositions have problems such as lack of molecular weight distribution basis, failure to consider the amino acid loss of hydrolyzed raw materials, and failure to reflect the synergistic effects of the composition in terms of taste, preparation and function.

[0008] Therefore, there is an urgent need to develop a composition with a reasonable peptide molecular weight distribution, sufficient nutrition, and multiple nutrients that synergistically improve the symptoms of infants with food protein allergies based on the sensitization mechanism. Summary of the invention

[0009] In one aspect, the present application provides a nutritional composition containing extensive hydrolyzed casein for infant or toddler formula, the nutritional composition comprising extensive hydrolyzed casein, solid corn syrup, lactose-N-neotetraose, 2'-fucosyllactose and Lactobacillus rhamnosus GG ( Lacticaseibacillus rhamnosus GG).

[0010] In some embodiments of the present application, the nutritional composition comprises, by weight, 80 to 240 parts of extensively hydrolyzed casein, 60 to 230 parts of solid corn syrup, 0.5 to 20 parts of lactose-N-neotetraose, 1 to 30 parts of 2'-fucosyllactose and 0.05 to 5 parts of Lactobacillus rhamnosus GG.

[0011] In one embodiment of the present application, the nutritional composition comprises, by weight, 157 parts of extensively hydrolyzed casein powder, 112.5 parts of solid corn syrup, 4.3 parts of lactose-N-neotetraose, 7.9 parts of 2'-fucosyllactose, and 0.5 parts of Lactobacillus rhamnosus GG.

[0012] In the above embodiment, more than 95% of the peptide molecular weight distribution in the deep hydrolyzed casein powder is less than 3000 Da; more than 85% of the peptide molecular weight distribution is less than 1000 Da; in some embodiments, the deep hydrolyzed casein powder can be supplemented with additional monomeric amino acids based on the CODEX amino acid score; in some embodiments, the lactose content in the deep hydrolyzed casein powder is less than 0.3%, which meets the definition of lactose-free. In some embodiments, the molecular weight distribution of peptides in the extensively hydrolyzed casein powder is 96% or more less than 3000 Da, 97% or more less than 3000 Da, 98% or more less than 3000 Da, 99.0% or more less than 3000 Da, 99.1% or more less than 3000 Da, 99.2% or more less than 3000 Da, 99.3% or more less than 3000 Da, 99.4% or more less than 3000 Da, 99.5% or more less than 3000 Da, 99.6% or more less than 3000 Da, 99.7% or more less than 3000 Da, 99.8% or more less than 3000 Da, or 99.9% or more less than 3000 Da; and / or, 85% or more account for ≤1000 Da, 86% or more account for ≤1000 Da, 87% or more account for ≤1000 Da, 88% or more account for ≤1000 Da, or 89% or more account for ≤1000 Da.

[0013] In the above embodiment, based on the nutritional composition, the content of casein, soy protein or whole milk protein in the extensively hydrolyzed casein powder is less than 2.5 mg / kg, and the content of β-lactoglobulin is less than 0.167 mg / kg; or the content of β-lactoglobulin is less than 5 mg / kg.

[0014] In the above embodiment, based on the amino acid pattern of the deeply hydrolyzed casein after deep hydrolysis, at least cysteine ​​and tryptophan are additionally supplemented; for example, at least 2.54 parts of cysteine ​​and 2.03 parts of tryptophan are additionally supplemented to ensure that the amino acid score is qualified and meet the normal growth needs of infants.

[0015] On the other hand, the present application provides an infant or toddler formula food comprising the above nutritional composition. The formula food may also include one or more of protein-free fat powder, docosahexaenoic acid, arachidonic acid and nucleotides;

[0016] Optionally, the protein-free fat powder refers to a fat powder produced by using edible oil as the main raw material, adding embedding materials, adding or not adding emulsifiers, antioxidants, acidity regulators and other food additives, and being processed by main processes such as batching, homogenization, sterilization, spray drying, and packaging, and using raw and auxiliary materials that do not contain protein; the protein content of the protein-free fat powder is ≤0.5g / 100g, and the fat content is ≥30g / 100g; the edible oil is derived from one or a combination of vegetable oils, animal oils, and other oils, and hydrogenated oils shall not be used; for example, the protein-free fat powder may contain glucose syrup, sunflower oil, low-erucic acid rapeseed oil, coconut oil, mixed tocopherol concentrate, ascorbyl palmitate, sodium octenyl succinate starch, mono / diglycerol fatty acid esters, sodium ascorbate; and / or, The docosahexaenoic acid may be DHA algae oil powder, and its raw material ingredients may also include sodium ascorbate, glyceryl monostearate, tricalcium phosphate, citric acid, phospholipids and glucose syrup; and / or, The arachidonic acid can be arachidonic acid powder, and its raw material components also include sodium ascorbate, glyceryl monostearate, tricalcium phosphate, citric acid and glucose syrup.

[0017] In some embodiments, the formula food is an extensively hydrolyzed infant milk protein formula food for special medical purposes.

[0018] In a third aspect, the present application provides the above-mentioned nutritional composition or its formula food for use without causing food protein allergy and / or gastrointestinal dysfunction in infants or young children.

[0019] Due to the adoption of the above scheme, the present invention has the following beneficial effects compared with the prior art: (1) The composition for improving infant food protein allergies provided by the present application contains deeply hydrolyzed casein. Compared with β-lactoglobulin and α-lactalbumin, casein is easier to digest and hydrolyze and has lower immunogenicity. Therefore, replacing the protein in food with casein is more conducive to improving infant allergic reactions. Based on the amino acid score of deeply hydrolyzed casein, additional monomeric amino acids are added to ensure that the essential amino acids meet the requirements. Deeply hydrolyzed casein basically does not cause allergic reactions. At the same time, it prevents infants with food protein allergies from aggravating symptoms such as diarrhea and gastrointestinal dysfunction due to lactose intolerance.

[0020] (2) The composition for improving infant food protein allergies provided in the present application is added with solid corn syrup, which significantly improves the overall poor taste caused by the exposure of bitter amino acids in deep hydrolyzed casein compared to maltodextrin. At the same time, in order to reduce the probability of gastrointestinal discomfort in allergic infants, lactose is not used as a carbohydrate in the composition.

[0021] (3) The composition for improving infant food protein allergy provided in the present application is added with lactose-N-neotetraose, 2'-fucosyllactose and rhamnosus lactobacillus GG, which can enhance the diversity of intestinal flora, promote the growth of rhamnosus lactobacillus GG, ensure its better function, and improve the intestinal discomfort caused by allergies; at the same time, both have immunomodulatory effects and can synergistically promote the immune health of infants.

[0022] In summary, the present invention can effectively improve infant food protein allergy and gastrointestinal dysfunction, enhance immunity, and promote normal physical development by combining raw materials with specific requirements and working together.

[0023] Other features and advantages of the present application will be described in the following description, and partly become apparent from the description, or be understood by implementing the present application. Other advantages of the present application can be realized and obtained by the schemes described in the description. DETAILED DESCRIPTION

[0024] The present application describes multiple embodiments, but the description is exemplary rather than restrictive, and it is obvious to a person skilled in the art that there may be more embodiments and implementations within the scope of the embodiments described in the present application.

[0025] Example 1 The composition for improving infant food protein allergy is applied to infant milk protein deep hydrolyzed formula food for special medical purposes. In parts by weight, the formula includes: 157 parts of deep hydrolyzed casein powder (Dai Weilin International Trade (Shanghai) Co., Ltd.), 2.54 parts of cysteine ​​and 2.03 parts of tryptophan, 649 parts of protein-free fat powder (Tianjin Yinhe Weiye Import and Export Co., Ltd.), 112.5 parts of solid corn syrup (Bolling Biotechnology Co., Ltd.), 4.3 parts of lactose-N-neotetraose, 7.9 parts of 2'-fucosyllactose, 0.5 parts of rhamnosus Lactobacillus casei GG (Hebei Chengxi Import and Export Trading Co., Ltd.), 9.4 parts of DHA algae oil powder (Guangdong Runke Biotechnology Co., Ltd.), 10.4 parts of arachidonic acid AA powder (Guangdong Runke Biotechnology Co., Ltd.), and 0.5 parts of nucleotides (20.3% adenosine 5'-monophosphate, 30% disodium 5'-cytidylate, 24% disodium 5'-uridylate, 6.9% disodium 5'-guanylate, and 13.8% disodium 5'-inosinate).

[0026] Example 2 The molecular weight distribution of the formula food in the above Example 1 was analyzed, and the results are shown in Table 1: Table 1 Molecular weight distribution

[0027] The results showed that the molecular weight distribution of peptides in this formula food was 99.9% less than 3000 Da, and more than 85% less than 1000 Da, effectively reducing the probability of allergic reactions.

[0028] Example 3 The allergenic protein content of the formula food in Example 1 was analyzed, and the results are shown in Table 2: Table 2 Allergic protein content

[0029] The detection adopts ELISA method, in which the detection limit of casein, soy protein and whole milk protein allergens is 2.5 mg / kg; the detection limit of β-lactoglobulin allergen is 0.167 mg / kg; the detection limit of β-lactoglobulin fragment allergen is 5 mg / kg. The results show that the protein allergen content of this formula food is lower than the detection limit, indicating that this composition can be used in infant formula food to reduce the risk of food protein allergy.

[0030] Example 4 The dissolution effects of each monomer (casein, solid corn syrup, 2'-fucosyllactose, lactose-N-neotetraose, Lactobacillus rhamnosus GG) in the compositions with different ratios and the overall composition were measured. The different ratios of the compositions are shown in Table 3, the scoring criteria are shown in Table 4, and the specific results are shown in Table 5.

[0031] Table 3 Compositions with different ratios

[0032] Table 4 Scoring criteria

[0033] Table 5 Analysis of brewing characteristics

[0034] The results showed that the organizational state and preparation characteristics of the final combination in the four groups were significantly better than those of each single component, which indicates that on the basis of ensuring that each component can exert its efficacy, they can play a synergistic role in preparation.

[0035] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A nutritional composition containing extensively hydrolyzed casein for infant or toddler formula, comprising extensively hydrolyzed casein, solid corn syrup, lactose-N-neotetraose, 2'-fucosyllactose and Lactobacillus rhamnosus GG ( Lacticaseibacillus rhamnosus GG).

2. The nutritional composition according to claim 1, comprising, by weight, 80 to 240 parts of extensively hydrolyzed casein, 60 to 230 parts of solid corn syrup, 0.5 to 20 parts of lactose-N-neotetraose, 1 to 30 parts of 2'-fucosyllactose and 0.05 to 5 parts of Lactobacillus rhamnosus GG.

3. The nutritional composition according to claim 2, comprising, by weight, 157 parts of extensively hydrolyzed casein powder, 112.5 parts of solid corn syrup, 4.3 parts of lactose-N-neotetraose, 7.9 parts of 2'-fucosyllactose and 0.5 parts of Lactobacillus rhamnosus GG.

4. The nutritional composition according to any one of claims 1 to 3, wherein More than 95% of the peptide molecular weight distribution in the deep hydrolyzed casein powder is less than 3000 Da; more than 85% of the peptide molecular weight distribution is less than 1000 Da; the lactose content is less than 0.3%, meeting the definition of lactose-free; Optionally, the molecular weight distribution of peptides in the deep hydrolyzed casein powder is 96% or more less than 3000 Da, 97% or more less than 3000 Da, 98% or more less than 3000 Da, 99.0% or more less than 3000 Da, 99.1% or more less than 3000 Da, 99.2% or more less than 3000 Da, 99.3% or more less than 3000 Da, 99.4% or more less than 3000 Da, 99.5% or more less than 3000 Da, 99.6% or more less than 3000 Da, 99.7% or more less than 3000 Da, 99.8% or more less than 3000 Da, or 99.9% or more less than 3000 Da; and / or, 85% or more account for ≤1000 Da, 86% or more account for ≤1000 Da, 87% or more account for ≤1000 Da, 88% or more account for ≤1000 Da, or 89% or more account for ≤1000 Da; Optionally, the extensively hydrolyzed casein powder is additionally supplemented with monomeric amino acids according to the CODEX amino acid score.

5. The nutritional composition according to any one of claims 1 to 4, wherein The content of casein, soy protein or whole milk protein in the deep hydrolyzed casein powder is less than 2.5 mg / kg, the content of beta-lactoglobulin is less than 0.167 mg / kg, and the content of beta-lactoglobulin is less than 5 mg / kg.

6. The nutritional composition according to any one of claims 1 to 5, wherein The extensively hydrolyzed casein powder at least additionally supplements cysteine ​​and tryptophan.

7. An infant or toddler formula comprising the nutritional composition of any one of claims 1 to 6.

8. The formula food according to claim 7, further comprising one or more of protein-free fat powder, docosahexaenoic acid, arachidonic acid and nucleotides; optionally, the docosahexaenoic acid can be DHA algae oil powder, and / or the arachidonic acid can be arachidonic acid powder.

9. The formula food according to claim 7 or 8, which is an infant milk protein extensively hydrolyzed formula food for special medical purposes.

10. Use of the nutritional composition according to any one of claims 1 to 6 or the formula food according to any one of claims 7 to 9 for not causing food protein allergy and / or gastrointestinal dysfunction in infants or young children.

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