PCR (Polymerase Chain Reaction) tube placing box capable of preventing mistaken sample adding

Abstract

The PCR tube placement box comprises a sample adding equipment main body, a PCR tube placement assembly slidably mounted at the top of the sample adding equipment main body, and a protection cover hinged to the left side of the sample adding equipment main body through a hinge; the PCR tube placement assembly comprises a plurality of sliding bins of strip-shaped structures, a first label strip is pasted to the right side of the top of each sliding bin, and a plurality of PCR tube placement grooves which are evenly distributed at equal intervals are formed in the left side of each first label strip. According to the PCR tube placement box capable of preventing mistaken sample adding, after an electromagnet I is electrified, a clamping plate is driven by the pulling force of a clamping spring to gradually rotate towards one side close to the inner wall of a PCR tube placement groove, so that a PCR tube clamping assembly is unlocked, and a lifting assembly and an electromagnet II are matched to be electrified, so that a plug inwards retracts into a telescopic sleeve; after unlocking, under the action of the reset spring, the push rod slides upwards, and the PCR tube at the top of the weighing sensor is pushed out.

Description

Technical Field

[0001] The invention relates to the field of molecular biotechnology, in particular to a PCR tube placement box for preventing wrong sample addition. Background Art

[0002] PCR is a molecular biology technique used to amplify specific DNA fragments. It refers to the process of replicating daughter DNA complementary to the parent DNA template in vitro through denaturation, annealing, and extension steps under the catalysis of DNA polymerase, using the parent DNA as a template and specific primers as the extension starting point. In the actual operation of PCR, since there are many system components involved, many reagents will be used. The PCR tube after sample addition can be directly placed in the placement box.

[0003] After searching, a pull-out PCR sample loading device with publication number CN210556414U is found, comprising: a box body, a box cover and a PCR tube holder; wherein the box body has an open top surface, and the top surface is provided with an open / closed box cover; the box body has an open side surface; the bottom surface of the box body is provided with a long straight pull-out track, and one end of the pull-out track extends to the open side surface; the upper part of the PCR tube holder is provided with a plurality of PCR tube holes, and the plurality of PCR tube holes are arranged in a vertical column along the length direction of the PCR tube holder; the PCR tube holes are used to place PCR tubes; the lower part of the PCR tube holder is movably connected to the pull-out track, and the PCR tube holder is pulled relative to the pull-out track along the length direction. The pull-out PCR sample loading device of the utility model can enhance the accuracy of sample loading.

[0004] However, in the implementation of relevant technologies, it was found that the pull-out PCR loading device of the above design has the following problems: by adding sequentially arranged marks on the PCR loading device to reduce missed and wrong additions, when the experimenter is faced with a large number of reagents, once the experimenter is not focused and misreads the position of the liquid collection port, or forgets the position of the reagent after the experiment is interrupted due to external force, there is still a possibility of adding the wrong sample. For this reason, we propose a PCR tube placement box that prevents wrong sample addition. Summary of the invention

[0005] The object of the present invention is to provide a PCR tube placement box for preventing wrong sample addition, so as to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an anti-misalignment sample loading device provided with a positioning clamping structure, comprising a sample loading device body, a PCR tube placement component slidably mounted on the top of the sample loading device body, and a protective cover hinged to the left side of the sample loading device body through a hinge, the PCR tube placement component can be used to place PCR tubes, and the protective cover can prevent foreign matter from entering the PCR tubes after being closed, a button is arranged on the left side of the top of the sample loading device body, and a display screen is arranged on one side of the button, a paper outlet is arranged on one side of the display screen, a printer is arranged at the rear end of the paper outlet, a microprocessor is arranged at the input end of the printer, and a memory is arranged at the output end of the microprocessor, a speaker for prompting is also connected to the output end of the microprocessor, the button is used to input the PCR tube number, and the microprocessor plays a central control role;

[0007] The PCR tube placement assembly includes a plurality of sliding bins of strip-shaped structures, and a label strip 1 is pasted on the right side of the top of the sliding bin, and a plurality of equidistant and evenly distributed PCR tube placement slots are arranged on the left side of the label strip 1;

[0008] A PCR tube clamping assembly is fixedly arranged inside each group of the PCR tube placement slots, and a weighing sensor is arranged below the PCR tube clamping assembly. A lifting assembly for pushing the PCR tube is arranged below the weighing sensor. When the PCR tube is placed inside the PCR tube placement slot, the weight of the PCR tube above is weighed by the weighing sensor. If the weight of the PCR tube increases, it means that the PCR tube has been added.

[0009] The lifting assembly includes a pushing rod fixed under the weighing sensor, and a guide slide is fixed under the pushing rod, a telescopic sleeve is sleeved on the outside of the pushing rod, an L-shaped limit slide is slidably installed under the guide slide, and a plug inserted in the telescopic sleeve is fixed to the end of the limit slide, a connecting spring is installed between the two groups of limit slides, and the left and right ends of the connecting spring are respectively provided with electromagnets 2 fixed on the two groups of limit slides and electrically connected to the microprocessor. When the PCR tube in the corresponding position needs to be taken out, the PCR tube can be pushed upward by the lifting assembly to push the PCR tube out of the PCR tube placement slot, which can avoid accidental removal caused by misreading by the experimenter.

[0010] As a further description of the above technical solution: a fixed socket is provided at the connection between the PCR tube placement assembly and the sample loading device body, and a semiconductor refrigeration plate fixed on the sample loading device body is arranged below the PCR tube placement assembly. The semiconductor refrigeration plate can cool the PCR tubes placed in the PCR tube placement assembly, and the fixed socket can position the PCR tube placement assembly.

[0011] As a further description of the above technical solution: the PCR tube placement component also includes a placement socket opened on the top of the PCR tube placement slot, and a one-to-one corresponding LED light is set on one side of the placement socket, and a label strip 2 is set on one side of the LED light. When the PCR tube needs to be taken or placed, the LED light on the corresponding PCR tube placement slot lights up, which can play a certain prompting role.

[0012] As a further description of the above technical solution: the surfaces of the label strip one and the label strip two are both provided with numbers, and the numbers on the label strip one and the label strip two constitute a two-digit number, the number on the label strip one is the number on the tens place, and the number on the label strip two is the number on the ones place, and the two-digit number formed by the numbers on the label strip one and the label strip two is the code of each PCR tube placement slot, and after entering the appropriate code by pressing the button, the PCR tube placement slot can be operated.

[0013] As a further description of the above technical solution: the button, microprocessor and LED light are electrically connected in sequence, the button can input codes into the microprocessor as an information input device, and the microprocessor can issue corresponding instructions to the output device in the corresponding PCR tube placement slot.

[0014] As a further description of the above technical solution: the PCR tube clamping assembly includes a clamping plate rotatably installed on the inner wall of the PCR tube placement slot through a rotating shaft, and a piston rod is installed between the clamping plate and the PCR tube placement slot through a pin shaft, and a clamping spring is sleeved on the surface of the piston rod, and an electromagnet is provided on the clamping plate and the PCR tube placement slot. By arranging the PCR tube clamping assembly inside the PCR tube placement slot, the PCR tube can be effectively clamped to prevent the PCR tube from shaking in the PCR tube placement slot.

[0015] As a further description of the above technical solution: one side of the clamping plate is elastically connected to a bonding plate, and a second rotating shaft is provided at the connection between the bonding plate and the clamping plate, and a supporting spring is installed between the bonding plate and the clamping plate. The bonding plate is further tightly attached to the PCR tube, which can increase the resistance between the PCR tube and the bonding plate. Only when the PCR tube clamping assembly is loosened can the experimenter smoothly take out the PCR tube placed inside.

[0016] As a further description of the above technical solution: the electromagnet 1 is electrically connected to the microprocessor. When the electromagnet 1 is energized, the clamping plate can be rotated toward the inner wall of the PCR tube placement slot, thereby unlocking the PCR tube clamping assembly.

[0017] As a further description of the above technical solution: the weighing sensor is electrically connected to the microprocessor, and the weighing sensor can weigh the weight of the PCR tube, so as to determine whether to add samples according to the weight change of the PCR tube.

[0018] As a further description of the above technical solution: the push rod is raised and lowered in the telescopic sleeve through a guide slide, and the plug is slidably connected to the telescopic sleeve. A return spring is installed between the guide slide and the telescopic sleeve. The guide slide guides the push rod. When the plug is inserted into the telescopic sleeve, the push rod can be driven to move upward.

[0019] It can be seen from the above description that the technical problem to be solved by this application can definitely be solved through the above technical solution of this application.

[0020] At the same time, through the above technical solutions, the present invention has at least the following beneficial effects:

[0021] The PCR tube placement assembly of the present invention can place multiple groups of PCR tubes side by side through the sliding bin, saving the use space of the laboratory table, and the LED lights on each group of PCR tube placement slots can remind the PCR tubes to be taken, so that the experimenter can find the location of the required PCR tube at the first time. At the same time, when the PCR tube needs to be taken, the corresponding number can be input by pressing a key, and the electromagnet one is controlled to be energized, so that the clamping plate is gradually rotated to the side close to the inner wall of the PCR tube placement slot under the pulling force of the clamping spring, thereby unlocking the PCR tube clamping assembly, and in conjunction with the lifting assembly, the electromagnet two is energized to retract the plug inwardly into the telescopic sleeve. After unlocking, the push rod slides upward under the action of the reset spring, and the PCR tube on the top of the weighing sensor is pushed out, further facilitating the experimenter to find the PCR tube at the required position;

[0022] The present invention can also detect the weight of the PCR tube when it is clamped by a weighing sensor. The weight value detected by the weighing sensor in the area where the PCR tube is taken out is 0. If the pressure value detected by the weighing sensors in other areas is 0, the microprocessor controls the speaker to issue an alarm, which can serve as a reminder in the first time, and further prevent the experimenter from taking the wrong PCR tube and adding the wrong sample. Each time the number is input, the microprocessor stores the information in the memory and controls the printer to print the number of the taken out PCR tube. The experimenter can check whether the wrong sample is added during the experiment according to the displayed PCR tube number, which is convenient for subsequent test inspection. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0024] Figure 2 This is a schematic diagram of the internal structure of the PCR tube placement slot of the present invention;

[0025] Figure 3 For the present invention Figure 2 The enlarged structural diagram at A in the middle;

[0026] Figure 4For the present invention Figure 2 The enlarged structural diagram at B in the middle;

[0027] Figure 5 It is a structural schematic diagram of the main body of the sample adding device of the present invention;

[0028] Figure 6 It is a structural schematic diagram of the workflow of the present invention.

[0029] In the figure: 1. Sample loading device body; 2. PCR tube placement assembly; 201. Placement jack; 202. PCR tube placement slot; 203. LED light; 204. Label strip 2; 205. Label strip 1; 206. Sliding bin; 3. Speaker; 4. Paper outlet; 5. Display screen; 6. Button; 7. Weighing sensor; 8. PCR tube clamping assembly; 801. Clamping plate; 802. Clamping spring; 803. Rotating shaft 1; 80 4. Piston rod; 805. Support spring; 806. Second rotating shaft; 807. Laminating plate; 808. First electromagnet; 9. Protective cover; 10. Semiconductor cooling plate; 11. Lifting assembly; 1101. Push rod; 1102. Telescopic sleeve; 1103. Plug; 1104. Limiting slide bar; 1105. Second electromagnet; 1106. Connecting spring; 1107. Guide slide; 1108. Reset spring; 12. Fixed socket. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Implementation Case 1

[0032] As attached Figure 1 , Figure 2 , Figure 4 and Figure 6As shown, the present invention provides a technical solution: an anti-misalignment sample loading device provided with a positioning clamping structure, comprising a sample loading device body 1, a PCR tube placement component 2 slidably mounted on the top of the sample loading device body 1, and a protective cover 9 hinged to the left side of the sample loading device body 1 through a hinge, the PCR tube placement component 2 can be used to place PCR tubes, and the protective cover 9 can prevent foreign matter from entering the PCR tubes after being closed, a button 6 is arranged on the left side of the top of the sample loading device body 1, and a display screen 5 is arranged on one side of the button 6, a paper outlet 4 is arranged on one side of the display screen 5, a printer is installed at the rear end of the paper outlet 4, a microprocessor is installed at the input end of the printer, and a memory is arranged at the output end of the microprocessor, and a speaker 3 for prompting is also connected to the output end of the microprocessor, the button 6 is used to input the PCR tube number, and the microprocessor plays a central control role;

[0033] A fixed socket 12 is provided at the connection between the PCR tube placement component 2 and the sample adding device body 1, and a semiconductor cooling sheet 10 fixed on the sample adding device body 1 is provided below the PCR tube placement component 2. The semiconductor cooling sheet 10 can cool down the PCR tube placed in the PCR tube placement component 2, and the fixed socket 12 can position the PCR tube placement component 2.

[0034] Since the lifting assembly 11 includes a push rod 1101 fixed below the weighing sensor 7, and a guide slide 1107 is fixed below the push rod 1101, a telescopic sleeve 1102 is sleeved on the outside of the push rod 1101, and an L-shaped limit slide 1104 is slidably installed below the guide slide 1107, and a plug 1103 inserted into the telescopic sleeve 1102 is fixed at the end of the limit slide 1104, a connecting spring 1106 is installed between the two groups of limit slides 1104, and the left and right ends of the connecting spring 1106 are respectively fixed on the two groups of limit slides 1104 and electrically connected to the microprocessor. 05. When the PCR tube at the corresponding position needs to be taken out, the lifting assembly 11 can be used to push the PCR tube upward so that the PCR tube is pushed out of the PCR tube placement slot 202, which can avoid the experimenter's mistaking for the tube. The push rod 1101 is lifted and lowered in the telescopic sleeve 1102 through the guide slide 1107, and the plug 1103 is slidably connected to the telescopic sleeve 1102. A return spring 1108 is installed between the guide slide 1107 and the telescopic sleeve 1102. The guide slide 1107 guides the push rod 1101. When the plug 1103 is inserted into the telescopic sleeve 1102, the push rod 1101 can be driven to move upward.

[0035] Embodiment 2

[0036] The solution in the first embodiment is further introduced below in combination with a specific working method, as described below for details:

[0037] like Figure 1 and Figure 5 As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, the PCR tube placement assembly 2 includes a plurality of sliding compartments 206 of strip-shaped structures, and a label strip 1 205 is pasted on the top right side of the sliding compartment 206, and a plurality of equidistant and evenly distributed PCR tube placement slots 202 are arranged on the left side of the label strip 1 205;

[0038] At the same time, a PCR tube clamping assembly 8 is fixedly arranged inside each group of PCR tube placement slots 202, and a weighing sensor 7 is arranged below the PCR tube clamping assembly 8. The weighing sensor 7 is electrically connected to the microprocessor. The weighing sensor 7 can weigh the weight of the PCR tube, so as to judge whether to add samples according to the weight change of the PCR tube. A lifting assembly 11 for pushing the PCR tube is arranged below the weighing sensor 7. When the PCR tube is placed inside the PCR tube placement slot 202, the weight of the PCR tube above is weighed by the weighing sensor 7. If the weight of the PCR tube increases, it means that the PCR tube has been added.

[0039] Since the PCR tube placement component 2 also includes a placement socket 201 opened on the top of the PCR tube placement slot 202, a corresponding LED light 203 is arranged on one side of the placement socket 201, and a label strip 204 is arranged on one side of the LED light 203. When it is necessary to take or place a PCR tube, the LED light 203 on the corresponding PCR tube placement slot 202 lights up, which can play a certain prompting role;

[0040] In addition, numbers are provided on the surfaces of label strip 1 205 and label strip 2 204, and the numbers on label strip 1 205 and label strip 2 204 constitute a two-digit number. The numbers on label strip 1 205 are the numbers on the tens place, and the numbers on label strip 2 204 are the numbers on the ones place. The two-digit number formed by the numbers on label strip 1 205 and label strip 2 204 is the code of each PCR tube placement slot 202. After inputting the appropriate code through button 6, the PCR tube placement slot 202 can be operated. Button 6, microprocessor and LED light 203 are electrically connected in sequence. Button 6 can input the code to the microprocessor as an information input device, and the microprocessor can issue corresponding instructions to the output device in the corresponding PCR tube placement slot 202.

[0041] Embodiment 3

[0042] The solution in the first embodiment is further introduced below in combination with a specific working method, as described below for details:

[0043] like Figure 2 and Figure 3As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, the PCR tube clamping assembly 8 includes a clamping plate 801 rotatably installed on the inner wall of the PCR tube placement slot 202 through a rotating shaft 803, and a piston rod 804 is installed between the clamping plate 801 and the PCR tube placement slot 202 through a pin shaft, and a clamping spring 802 is sleeved on the surface of the piston rod 804. The clamping plate 801 and the PCR tube placement slot 202 are both provided with an electromagnet 808. By arranging the PCR tube clamping assembly 8 inside the PCR tube placement slot 202, the PCR tube can be effectively clamped to prevent the PCR tube from shaking in the PCR tube placement slot 202. One side of the clamping plate 801 is elastically connected to a fitting plate 807, and a rotating shaft 2 806 is provided at the connection between the fitting plate 807 and the clamping plate 801, and a supporting spring 805 is installed between the fitting plate 807 and the clamping plate 801. The fitting plate 807 is further in close contact with the PCR tube, which can increase the resistance between the PCR tube and the fitting plate 807. Only when the PCR tube clamping assembly 8 is loosened can the experimenter smoothly take out the PCR tube placed inside. The electromagnet 1 808 is electrically connected to the microprocessor. When the electromagnet 1 808 is energized, the clamping plate 801 can be rotated toward the inner wall of the PCR tube placement slot 202, thereby unlocking the PCR tube clamping assembly 8.

[0044] The implementation process of the above technical solution is:

[0045] When it is necessary to add samples, the experimenter inputs the number of the required PCR tube by pressing button 6. The number is a two-digit number composed of label strip 1 205 and label strip 2 204 at the corresponding position of the PCR tube placement slot 202. After input, the microprocessor obtains the number and controls the LED light 203 in the corresponding number area to light up to remind the experimenter to take out the PCR tube and add samples. During this process, the LED light 203 remains on until the PCR tube is reinserted and the LED light 203 is turned off. When the PCR tube is inserted, the bottom of the PCR tube contacts the weighing sensor 7 and moves to Push the push rod 1101 downward, at this time, the electromagnet 1105 is in the power-off state, and the push rod 1101 can drive the limit slide bar 1104 to move downward through the guide slide plate 1107 until the plug 1103 reaches the insertion hole position of the telescopic sleeve 1102. Since the electromagnet 1105 is in the power-off state, the limit slide bar 1104 can be pushed to slide to the left and right sides under the action of the connecting spring 1106, so that the plug 1103 is inserted into the jack, locking the weighing sensor 7, and the weighing sensor 7 can weigh the weight of the PCR tube, and after transmitting it to the microprocessor, it is recorded in the memory;

[0046] At this time, the electromagnet 808 is powered off, and the PCR tube clamping assembly 8 in the PCR tube placement slot 202 clamps the PCR tube to a limited position. Since the clamping plate 801 is a conical structure, when the PCR tube is inserted, the clamping plate 801 is pressed toward both sides, thereby compressing the clamping spring 802 and the piston rod 804, so that the clamping plate 801 rotates toward the side close to the PCR tube placement slot 202, and the fitting plate 807 rotates toward the side close to the clamping plate 801 at the same time, and compresses the support spring 805, so that the PCR tube can be clamped;

[0047] When it is necessary to take out the PCR tube, the number of the desired PCR tube can be input by pressing button 6. At this time, both the electromagnet 2 1105 and the electromagnet 1 808 are energized. After the electromagnet 1 808 is energized, the clamping plate 801 gradually rotates toward the inner wall of the PCR tube placement slot 202 under the pulling force of the clamping spring 802, so that the clamping plate 801 is away from the PCR tube. At the same time, after the electromagnet 2 1105 is energized, the electromagnet 2 1105 is attracted inwardly, driving the connecting spring 1106 to compress inwardly, so that the plug 1103 is separated from the socket of the telescopic sleeve 1102. position, and under the action of the reset spring 1108, the push rod 1101 slides upward to push out the PCR tube on the top of the weighing sensor 7. The experimenter can quickly find the PCR tube at the required position to achieve secondary sample addition. After sample addition, the corresponding PCR tube placement slot 202 is repeatedly inserted. At this time, the weighing sensor 7 in the PCR tube placement slot 202 weighs the weight of the PCR tube again. If the weight of the PCR tube increases, it means that the sample is added correctly. If the weight of the PCR tube does not increase, the microprocessor controls the speaker 3 to issue a reminder to prompt the experimenter to add the sample.

[0048] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A dislocation-proof sample loading device provided with a positioning clamping structure, comprising a sample loading device body (1), a PCR tube placement assembly (2) slidably mounted on the top of the sample loading device body (1), and a protective cover (9) hinged to the left side of the sample loading device body (1) through a hinge, characterized in that: A button (6) is arranged on the left side of the top of the sample adding device body (1), and a display screen (5) is arranged on one side of the button (6), a paper outlet (4) is arranged on one side of the display screen (5), a printer is installed at the rear end of the paper outlet (4), a microprocessor is installed at the input end of the printer, and a memory is arranged at the output end of the microprocessor, and a speaker (3) for prompting is also connected to the output end of the microprocessor; The PCR tube placement assembly (2) comprises a plurality of sliding chambers (206) of strip-shaped structures, and a label strip 1 (205) is pasted on the top right side of the sliding chamber (206), and a plurality of equidistant and evenly distributed PCR tube placement slots (202) are arranged on the left side of the label strip 1 (205); A PCR tube clamping assembly (8) is fixedly arranged inside each group of the PCR tube placement slots (202), and a weighing sensor (7) is arranged below the PCR tube clamping assembly (8), and a lifting assembly (11) for pushing the PCR tube is arranged below the weighing sensor (7); The lifting assembly (11) comprises a pushing rod (1101) fixed below the weighing sensor (7), and a guide slide (1107) is fixed below the pushing rod (1101), a telescopic sleeve (1102) is sleeved on the outside of the pushing rod (1101), an L-shaped limit slide bar (1104) is slidably installed below the guide slide bar (1107), and a plug (1103) inserted into the telescopic sleeve (1102) is fixed at the end of the limit slide bar (1104), a connecting spring (1106) is installed between two groups of the limit slide bars (1104), and the left and right ends of the connecting spring (1106) are respectively provided with electromagnets (1105) fixed on the two groups of limit slide bars (1104) and electrically connected to the microprocessor.

2. The anti-misalignment sample loading device with a positioning and clamping structure according to claim 1, characterized in that: A fixed plug hole (12) is provided at the connection between the PCR tube placement component (2) and the sample adding device body (1), and a semiconductor cooling plate (10) fixed on the sample adding device body (1) is provided below the PCR tube placement component (2).

3. The anti-misalignment sample adding device with a positioning and clamping structure according to claim 2, characterized in that: The PCR tube placement assembly (2) further comprises a placement socket (201) provided at the top of the PCR tube placement slot (202), one side of the placement socket (201) being provided with a one-to-one corresponding LED light (203), and one side of the LED light (203) being provided with a second label strip (204).

4. The anti-misalignment sample adding device with a positioning and clamping structure according to claim 3, characterized in that: The surfaces of the label strip one (205) and the label strip two (204) are both provided with numbers, and the numbers on the label strip one (205) and the label strip two (204) constitute a two-digit number, the numbers on the label strip one (205) are the numbers on the tens place, and the numbers on the label strip two (204) are the numbers on the ones place.

5. The anti-misalignment sample adding device with a positioning and clamping structure according to claim 4, characterized in that: The button (6), microprocessor and LED light (203) are electrically connected in sequence.

6. The anti-dislocation sample loading device with a positioning and clamping structure according to claim 1, characterized in that: The PCR tube clamping assembly (8) comprises a clamping plate (801) rotatably mounted on the inner wall of a PCR tube placement slot (202) via a rotating shaft (803), and a piston rod (804) is mounted between the clamping plate (801) and the PCR tube placement slot (202) via a pin shaft, a clamping spring (802) is sleeved on the surface of the piston rod (804), and an electromagnet (808) is provided on both the clamping plate (801) and the PCR tube placement slot (202).

7. The anti-misalignment sample loading device with a positioning and clamping structure according to claim 6, characterized in that: One side of the clamping plate (801) is elastically connected to a bonding plate (807), and a second rotating shaft (806) is provided at the connection between the bonding plate (807) and the clamping plate (801), and a supporting spring (805) is installed between the bonding plate (807) and the clamping plate (801).

8. The anti-misalignment sample loading device with a positioning and clamping structure according to claim 7, characterized in that: The electromagnet 1 (808) is electrically connected to the microprocessor.

9. The anti-misalignment sample loading device with a positioning and clamping structure according to claim 1, characterized in that: The weighing sensor (7) is electrically connected to the microprocessor.

10. The anti-dislocation sample loading device with a positioning and clamping structure according to claim 1, characterized in that: The push rod (1101) is lifted and lowered in the telescopic sleeve (1102) via a guide slide (1107), and the plug (1103) is slidably connected to the telescopic sleeve (1102), and a return spring (1108) is installed between the guide slide (1107) and the telescopic sleeve (1102).

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