Cell culture equipment and electrical stimulation assembly thereof

Abstract

The invention provides cell culture equipment and an electrical stimulation assembly thereof, and belongs to the technical field of cell culture.The cell culture equipment comprises a dish body and a dish cover, a plurality of culture chambers are formed in the dish body, containing chambers are formed in the dish cover, the number of the containing chambers is the same as that of the culture chambers, and the positions of the containing chambers correspond to those of the culture chambers. Comprising an electrical stimulator, and further comprises an electrical stimulation mechanism and an adjusting mechanism, through the arrangement of an iron core and a magnet piece, when the electrical stimulator is powered on to work, current passes through a coil, the iron core generates magnetic force, the magnetic force of the iron core pushes the magnet piece to move in the direction away from the iron core, and the magnet piece drives two conductive columns to move downwards synchronously; the first electrode and the second electrode are driven to move downwards synchronously, so that the vertical positions of the first electrode and the second electrode are adjusted, cells with different depths in a culture solution are electrically stimulated, and the cell culture quality is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of cell culture, and in particular relates to a cell culture device and an electrical stimulation component thereof. Background Art

[0002] Cell culture technology is the cornerstone of modern biomedical research, which allows scientists to study the growth, differentiation and function of cells in vitro. As a non-invasive stimulation method, electrical stimulation has been widely used in cell culture to promote cell migration, proliferation and differentiation, as well as tissue growth.

[0003] A Chinese patent with application number CN201880067680.5 discloses a cell stimulation device, which includes: an electron emission element, which supplies charge to the aforementioned culture medium via a gas phase; a charge recovery electrode, which recovers charge from the aforementioned culture medium; the aforementioned electron emission element includes a bottom electrode, a surface electrode and an intermediate layer arranged between the bottom electrode and the surface electrode, and the aforementioned charge recovery electrode is arranged to be able to contact the aforementioned culture medium. The invention supplies the charge generated by the electron emission element to the culture medium via a gas phase, which can inhibit the electrolysis of water contained in the culture medium and inhibit changes in the component composition of the culture medium. However, when electrically stimulating cell culture, the potential difference near the positive and negative electrodes is large, resulting in a large electrical stimulation intensity near the positive and negative electrodes, resulting in uneven cell stimulation, inconsistent cell migration and growth factor expression, and affecting the quality of cell culture.

[0004] Therefore, in order to solve the above problems, it is necessary to provide a cell culture device and an electrical stimulation component thereof. Summary of the invention

[0005] The purpose of the present invention is to provide a cell culture device and its electrical stimulation component, aiming to solve the problem in the prior art that the potential difference near the positive and negative electrodes is large, resulting in a large electrical stimulation intensity near the positive and negative electrodes, uneven cell stimulation, resulting in inconsistent cell migration and growth factor expression, and affecting the quality of cell culture.

[0006] To achieve the above object, the present invention provides the following technical solutions: A cell culture device comprises a dish body and a dish cover, wherein a plurality of culture chambers are provided inside the dish body, and a plurality of accommodating chambers are provided inside the dish cover, the number of which is the same as that of the culture chambers and the positions of which are corresponding to those of the culture chambers.

[0007] An electrical stimulation assembly, comprising an electrical stimulation device, further comprising: An electrical stimulation mechanism, the electrical stimulation mechanism is arranged inside each of the containing chambers, the electrical stimulation mechanism comprises a first electrode and a second electrode, each of the first electrode and the second electrode is electrically connected to the electrical stimulation instrument, and is used to electrically stimulate the cells inside each of the culture chambers during cell culture; The adjusting mechanism is arranged inside each of the containing chambers, each of the electric stimulation mechanisms is connected to the dish cover via the corresponding adjusting mechanism, and the adjusting mechanism is used to adjust the positions of the first electrode and the second electrode inside the culture chamber.

[0008] Preferably, the adjustment mechanism includes a accommodating chamber fixedly connected to the dish cover, an iron core fixedly connected to the dish cover is provided inside the accommodating chamber, a coil is provided on the outer wall of the iron core, both ends of the coil are electrically connected to a first conductive member fixedly connected to the dish cover, and the two first conductive members are symmetrically arranged.

[0009] Preferably, two symmetrically arranged second conductive members are fixedly connected to the inner wall of the accommodating bin, and the two second conductive members correspond to the positions of the two first conductive members respectively. A first slide groove is provided on the top of the two second conductive members, and a first conductive member electrically connected to the corresponding second conductive member is slidably connected inside each of the first slide grooves, and a first elastic member is connected between a side of each of the first conductive members close to the accommodating bin and the corresponding inner wall of the first slide groove.

[0010] Preferably, the bottom of the accommodating bin is fixedly connected to two adjusting bins, and the two adjusting bins correspond to the positions of the two second conductive members respectively. A limiting ring is fixedly connected to the inside of the bottom of each adjusting bin, and a conductive column is provided inside each adjusting bin for sliding connection with the inner side of the corresponding limiting ring. A movable plate is fixedly connected to the outer wall of each conductive column for sliding connection with the corresponding inner wall of the adjusting bin, and a second elastic member is connected between the bottom of each movable plate and the top of the corresponding limiting ring. A second slide groove is provided inside each conductive column, and a second conductive member electrically connected to the corresponding conductive column is slidably connected inside each second slide groove, and a third elastic member is connected between the bottom of each second conductive member and the inner wall of the corresponding second slide groove.

[0011] Preferably, a second conductive groove is formed at the bottom of each of the two second conductive members, and the two second conductive grooves respectively correspond to the positions of the two second conductive members, and a first conductive groove is formed on the side of each of the two first conductive members away from each other, and the two first conductive grooves respectively correspond to the positions of the two first conductive members.

[0012] Preferably, a limiting groove is provided inside the iron core, and a limiting member is slidably connected inside the limiting groove. The limiting member passes through the bottom end of the iron core and is fixedly connected with a magnet member. The magnet member is fixedly connected to the outer walls of the two conductive columns. When current passes through the coil, the iron core can generate magnetic force, which can push the magnet member to move in a direction away from the iron core.

[0013] Preferably, a guide groove is provided on the outer wall of each conductive column, and a guide member slidably connected to the corresponding guide groove is fixedly connected to the inner side of each limit ring. When the magnet member drives the two conductive columns to move, the two guide members can move inside the corresponding guide groove, thereby enabling the two corresponding conductive columns to rotate synchronously.

[0014] Preferably, a partition plate is fixedly connected to the top of the connection between the magnet member and the two conductive columns, and the two partition plates are respectively arranged between two adjacent first conductive members and the second conductive members. A connecting groove is opened inside each of the partition plates, and the two first conductive members can move inside the corresponding connecting groove. An extrusion member fixedly connected to the partition plate is provided inside each of the connecting grooves. When the magnet member moves in a direction away from the iron core, the extrusion member can contact with the corresponding first conductive member and can extrude the first conductive member to move in a direction away from the first conductive member.

[0015] Preferably, the bottoms of the two conductive posts are fixedly connected to the first electrode and the second electrode respectively, and the two conductive posts can supply power to the first electrode and the second electrode when energized, and the two second conductive parts are electrically connected to the positive and negative output ports of the electrical stimulator respectively through cables.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention arranges an iron core and a magnet. When the electrical stimulator is powered on, current passes through the coil, which will cause the iron core to generate magnetic force. The magnetic force of the iron core will push the magnet to move away from the iron core, so that the magnet drives the two conductive columns to move downward synchronously, and drives the first electrode and the second electrode to move downward synchronously, thereby adjusting the vertical positions of the first electrode and the second electrode, achieving electrical stimulation of cells at different depths in the culture medium, and improving the quality of cell culture.

[0017] 2. The present invention provides guide grooves and guide members. When the magnet drives the two conductive pillars to move up and down, the two guide members can move inside the corresponding guide grooves, so that the two corresponding conductive pillars can rotate synchronously in one direction, driving the first electrode and the second electrode to rotate. Thus, during the up and down movement of the first electrode and the second electrode, the angle of the first electrode and the second electrode can be adjusted, and the moving range of the first electrode and the second electrode can be increased. At the same time, the rotation of the first electrode and the second electrode can stir the culture medium inside the culture chamber, so that the cells inside the culture medium move, thereby making the degree of electrical stimulation of the cells more uniform. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a structural schematic diagram of the dish body of the present invention; Figure 3 It is a structural schematic diagram of the dish cover of the present invention; Figure 4 It is a schematic diagram of the overall structure of the regulating mechanism and the electrical stimulation mechanism of the present invention; Figure 5 It is a schematic diagram of the internal structure of the regulating mechanism of the present invention; Figure 6 For the present invention Figure 5 A magnified view of the structure of the middle part A; Figure 7 It is a structural schematic diagram of the position limiting member of the present invention; Figure 8 It is a structural schematic diagram of the guide groove of the present invention; Fig. 9 It is a structural schematic diagram of the guide member of the present invention; Fig.10 It is an enlarged view of the structure of the partition plate of the present invention; Fig.11 It is a cross-sectional view of the overall structure of the present invention.

[0019] In the figure: 1, dish body; 11, dish cover; 12, culture chamber; 13, receiving chamber; 2, electric stimulator; 3, electric stimulation mechanism; 31, first electrode; 32, second electrode; 4, adjustment mechanism; 41, receiving chamber; 42, iron core; 43, coil; 44, first conductive member; 45, second conductive member; 46, first slide groove; 47, first conductive member; 48, first elastic member; 49, first conductive groove; 410, adjustment mechanism Warehouse; 411, limiting ring; 412, conductive column; 413, movable plate; 414, second elastic member; 415, second slide groove; 416, second conductive member; 417, third elastic member; 418, second conductive groove; 419, limiting groove; 420, limiting member; 421, magnet member; 422, partition plate; 423, connecting groove; 424, guide groove; 425, guide member; 426, extrusion member; 5, cable. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Embodiment 1 During electrical stimulation of cell culture, the positive and negative electrodes are in a fixed state, and due to the large potential difference near the positive and negative electrodes, the electrical stimulation intensity near the positive and negative electrodes will be larger, resulting in differences in the degree of stimulation received by the cells in the culture dish. The electrical stimulation intensity received by cells close to the positive and negative electrodes is higher than that of cells in other positions, resulting in inconsistent cell migration and growth factor expression, affecting the quality of cell culture.

[0022] See also Figure 1 - Figure 7 and Fig.11 A cell culture device comprises a dish body 1 and a dish cover 11, wherein a plurality of culture chambers 12 are provided inside the dish body 1, and a plurality of receiving chambers 13 are provided inside the dish cover 11, which are the same in number and corresponding in position as the culture chambers 12; An electrical stimulation component, applied to a cell culture device, comprises an electrical stimulation device 2, and further comprises: The electrical stimulation mechanism 3 is arranged inside each containing chamber 13, and includes a first electrode 31 and a second electrode 32. Each of the first electrode 31 and the second electrode 32 is electrically connected to the electrical stimulation device 2, and is used to electrically stimulate the cells inside each culture chamber 12 during cell culture; The adjusting mechanism 4 is arranged inside each containing chamber 13, and each electric stimulation mechanism 3 is connected to the dish cover 11 through the corresponding adjusting mechanism 4, and the adjusting mechanism 4 is used to adjust the position of the first electrode 31 and the second electrode 32 inside the culture chamber 12; The first electrode 31 and the second electrode 32 in each electrical stimulation mechanism 3 are electrically connected to the positive and negative output ports of the electrical stimulator 2, respectively. The electrical stimulator 2 is used to provide electrical stimulation of different waveforms, including square waves, sine waves, exponential waves, triangular waves, etc., can adjust the current size and frequency, and has a timing function. The electrical stimulator 2, the first electrode 31 and the second electrode 32 are all existing technologies and will not be repeated here.

[0023] like Figure 4-Figure 7 As shown, the adjustment mechanism 4 includes a accommodating chamber 41 fixedly connected to the dish cover 11, an iron core 42 fixedly connected to the dish cover 11 is provided inside the accommodating chamber 41, a coil 43 is provided on the outer wall of the iron core 42, and both ends of the coil 43 are electrically connected to a first conductive member 44 fixedly connected to the dish cover 11, and the two first conductive members 44 are symmetrically arranged; The inner wall of the accommodating bin 41 is fixedly connected with two symmetrically arranged second conductive members 45, and the positions of the two second conductive members 45 correspond to the positions of the two first conductive members 44 respectively. The tops of the two second conductive members 45 are provided with first slide grooves 46, and the interior of each first slide groove 46 is slidably connected with a first conductive member 47 electrically connected to the corresponding second conductive member 45, and a first elastic member 48 is connected between a side of each first conductive member 47 close to the accommodating bin 41 and the inner wall of the corresponding first slide groove 46, and a first conductive slot 49 is provided on the side of the two first conductive members 44 away from each other, and the two first conductive slots 49 correspond to the positions of the two first conductive members 47 respectively; The bottom of the accommodating bin 41 is fixedly connected with two adjusting bins 410, and the positions of the two adjusting bins 410 correspond to the positions of the second conductive member 45. The bottom interior of each adjusting bin 410 is fixedly connected with a limiting ring 411, and the interior of each adjusting bin 410 is provided with a conductive column 412 which is slidably connected to the inner side of the corresponding limiting ring 411. The outer wall of each conductive column 412 is fixedly connected with a movable plate 413 which is slidably connected to the inner wall of the corresponding adjusting bin 410. A second elastic member 414 is connected between the bottom of each movable plate 413 and the top of the corresponding limiting ring 411. A second sliding groove 415 is provided inside each conductive column 412, and a second conductive member 416 which is electrically connected to the corresponding conductive column 412 is slidably connected inside each second sliding groove 415. A third elastic member 417 is connected between the bottom of each second conductive member 416 and the inner wall of the corresponding second sliding groove 415. The bottoms of the two second conductive members 45 are each provided with a second conductive slot 418 , and the two second conductive slots 418 correspond to the positions of the two second conductive members 416 , respectively; The iron core 42 has a limit groove 419 formed inside, and a limit piece 420 is slidably connected inside the limit groove 419. The limit piece 420 passes through the bottom of the iron core 42 and is fixedly connected with a magnet piece 421. The magnet piece 421 is fixedly connected to the outer walls of the two conductive pillars 412. When current passes through the coil 43, the iron core 42 generates magnetic force, which can push the magnet piece 421 to move away from the iron core 42. It is a prior art that current passes through the coil 43 to make the iron core 42 generate magnetic force, which will not be elaborated. After the iron core 42 generates magnetic force, when the bottom of the iron core 42 is the N pole, the top of the magnet part 421 should also be set as the N pole. Conversely, when the bottom of the iron core 42 is the S pole, the top of the magnet part 421 should also be set as the S pole.

[0024] like Fig.10 As shown, a partition plate 422 is fixedly connected to the top of the connection between the magnet member 421 and the two conductive columns 412. The two partition plates 422 are respectively arranged between two adjacent first conductive members 44 and second conductive members 45. A connecting groove 423 is provided inside each partition plate 422. The two first conductive members 47 can move inside the corresponding connecting groove 423. An extrusion member 426 fixedly connected to the partition plate 422 is provided inside each connecting groove 423. When the magnet member 421 moves in a direction away from the iron core 42, the extrusion member 426 can contact with the corresponding first conductive member 47 and can extrude the first conductive member 47 to move in a direction away from the first conductive member 44. One end of the first conductive member 47 close to the first conductive member 44 can be spherical to facilitate the extrusion member 426 to push the first conductive member 47 to move. When the magnet member 421 moves to the extreme position away from the iron core 42, the first conductive member 47 can contact the partition plate 422, and no current passes through the coil 43.

[0025] The bottoms of the two conductive posts 412 are fixedly connected to the first electrode 31 and the second electrode 32 respectively. When the two conductive posts 412 are powered on, they can supply power to the first electrode 31 and the second electrode 32. The two second conductive members 45 are electrically connected to the positive and negative output ports of the electrical stimulator 2 through the cable 5 respectively. In the initial state, that is, when the electrical stimulator 2 is not powered on, the two second conductive parts 416 can be inserted into the corresponding second conductive slots 418, the two third elastic parts 417 are in a compressed state, the tops of the two conductive columns 412 are in contact with the bottoms of the corresponding second conductive parts 45, and the two first conductive parts 47 can pass through the corresponding connecting slots 423 and be inserted into the corresponding first conductive slots 49 under the action of the two first elastic parts 48, so that the second conductive part 45 and the coil 43 form a closed loop. In actual use, when the electrical stimulator 2 is powered on, the current passes through The coil 43 will cause the iron core 42 to generate a magnetic force. Under the principle of like attracts like and opposites repel like, the magnetic force of the iron core 42 will push the magnet part 421 to move in a direction away from the iron core 42, so that the magnet part 421 drives the two conductive pillars 412 to move downward synchronously, so that the movable plate 413 squeezes the second elastic member 414. The movement of the two conductive pillars 412 can drive the first electrode 31 and the second electrode 32 to move downward synchronously, thereby adjusting the vertical positions of the first electrode 31 and the second electrode 32, thereby achieving electrical stimulation of cells at different depths in the culture medium and improving the quality of cell culture.

[0026] When the magnet 421 moves in the direction away from the iron core 42, the second conductive member 416 can always be in contact with the inner wall of the second conductive slot 418 to be energized under the elastic reset action of the third elastic member 417, so as to avoid power failure when the first electrode 31 and the second electrode 32 move and affect the efficiency. When the magnet 421 moves to the extreme position, the first conductive member 47 can only contact the partition plate 422, and no current passes through the coil 43. At this time, the second elastic member 414 drives the conductive column 412 and the magnet 421 to reset. Subsequently, the current passes through the coil 43 again, and the magnet 421 moves down again, thereby realizing the reciprocating adjustment of the vertical position of the first electrode 31 and the second electrode 32.

[0027] The present invention arranges the iron core 42 and the magnet 421. When the electrical stimulator 2 is powered on, the current passes through the coil 43, which will cause the iron core 42 to generate magnetic force. The magnetic force of the iron core 42 will push the magnet 421 to move away from the iron core 42, so that the magnet 421 drives the two conductive columns 412 to move downward synchronously, and drives the first electrode 31 and the second electrode 32 to move downward synchronously, thereby adjusting the vertical positions of the first electrode 31 and the second electrode 32, thereby achieving electrical stimulation of cells at different depths in the culture medium and improving the quality of cell culture.

[0028] Embodiment 2 Based on the above embodiment, the first electrode and the second electrode can only adjust their positions in the vertical direction, and the range is limited, resulting in uneven electrical stimulation of cells inside the culture medium. Especially when a high-viscosity culture medium is used, the uneven electrical stimulation phenomenon is more obvious.

[0029] See also Figure 7 - Fig. 9 A guide groove 424 is provided on the outer wall of each conductive column 412, and a guide member 425 is fixedly connected to the inner side of each limiting ring 411 and is slidably connected to the corresponding guide groove 424. When the magnet member 421 drives the two conductive columns 412 to move, the two guide members 425 can move inside the corresponding guide groove 424, so that the two corresponding conductive columns 412 can rotate synchronously.

[0030] like Figure 8 As shown, the guide groove 424 is arranged in a wave shape. When the magnet member 421 drives the two conductive pillars 412 to move up and down, the guide members 425 can move inside the guide groove 424, and can make the corresponding conductive pillars 412 move in one direction.

[0031] During actual use, when the magnet 421 drives the two conductive pillars 412 to move up and down, the two guide members 425 can move inside the corresponding guide grooves 424, so that the two corresponding conductive pillars 412 can rotate synchronously in one direction, driving the first electrode 31 and the second electrode 32 to rotate. Therefore, during the up and down movement of the first electrode 31 and the second electrode 32, the angle of the first electrode 31 and the second electrode 32 can be adjusted, and the movement range of the first electrode 31 and the second electrode 32 can be increased. At the same time, through the rotation of the first electrode 31 and the second electrode 32, the culture medium inside the culture chamber 12 can be stirred, so that the cells inside the culture medium move, thereby making the degree of electrical stimulation of the cells more uniform.

[0032] The present invention provides a guide groove 424 and a guide member 425. When the magnet member 421 drives the two conductive pillars 412 to move up and down, the two guide members 425 can move inside the corresponding guide groove 424, so that the two corresponding conductive pillars 412 can rotate synchronously in one direction, driving the first electrode 31 and the second electrode 32 to rotate. Therefore, when the first electrode 31 and the second electrode 32 move up and down, the angle of the first electrode 31 and the second electrode 32 can be adjusted, and the movement range of the first electrode 31 and the second electrode 32 is increased. At the same time, the culture medium inside the culture chamber 12 can be stirred through the rotation of the first electrode 31 and the second electrode 32, so that the cells inside the culture medium move, thereby making the degree of electrical stimulation of the cells more uniform.

[0033] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture device, comprising a vessel body (1) and a vessel cover (11), characterized in that: A plurality of culture chambers (12) are provided inside the dish body (1), and a plurality of accommodating chambers (13) of the same number and corresponding positions as the culture chambers (12) are provided inside the dish cover (11).

2. An electrical stimulation component, applied to the cell culture device according to claim 1, comprising an electrical stimulation device (2), characterized in that: Also includes: an electrical stimulation mechanism (3), the electrical stimulation mechanism (3) being arranged inside each of the containing chambers (13), the electrical stimulation mechanism (3) comprising a first electrode (31) and a second electrode (32), each of the first electrode (31) and the second electrode (32) being electrically connected to the electrical stimulation device (2), and being used to electrically stimulate the cells inside each of the culture chambers (12) when cell culture is being performed; An adjusting mechanism (4), wherein the adjusting mechanism (4) is arranged inside each of the containing chambers (13), and each of the electrical stimulation mechanisms (3) is connected to the dish cover (11) via the corresponding adjusting mechanism (4), and the adjusting mechanism (4) is used to adjust the positions of the first electrode (31) and the second electrode (32) inside the culture chamber (12).

3. The electrical stimulation assembly according to claim 2, characterized in that: The regulating mechanism (4) comprises a containing chamber (41) fixedly connected to the dish cover (11); an iron core (42) fixedly connected to the dish cover (11) is provided inside the containing chamber (41); a coil (43) is provided on the outer wall of the iron core (42); both ends of the coil (43) are electrically connected to a first conductive member (44) fixedly connected to the dish cover (11); and the two first conductive members (44) are symmetrically arranged.

4. The electrical stimulation assembly according to claim 3, characterized in that: The inner wall of the accommodating bin (41) is fixedly connected with two symmetrically arranged second conductive members (45), the two second conductive members (45) respectively correspond to the positions of the two first conductive members (44), the tops of the two second conductive members (45) are each provided with a first slide groove (46), the interior of each first slide groove (46) is slidably connected with a first conductive member (47) electrically connected to the corresponding second conductive member (45), and a first elastic member (48) is connected between a side of each first conductive member (47) close to the accommodating bin (41) and the inner wall of the corresponding first slide groove (46).

5. The electrical stimulation assembly according to claim 4, characterized in that: The bottom of the accommodating chamber (41) is fixedly connected to two regulating chambers (410), the two regulating chambers (410) respectively corresponding to the positions of the two second conductive members (45), the bottom of each regulating chamber (410) is fixedly connected to a limit ring (411), the inside of each regulating chamber (410) is provided with a conductive column (412) slidably connected to the inner side of the corresponding limit ring (411), and the outer wall of each conductive column (412) is fixedly connected to a movable plate slidably connected to the inner wall of the corresponding regulating chamber (410). (413), a second elastic member (414) is connected between the bottom of each movable plate (413) and the top of the corresponding limiting ring (411), a second slide groove (415) is opened inside each conductive column (412), a second conductive member (416) electrically connected to the corresponding conductive column (412) is slidably connected inside each second slide groove (415), and a third elastic member (417) is connected between the bottom of each second conductive member (416) and the inner wall of the corresponding second slide groove (415).

6. The electrical stimulation assembly according to claim 5, characterized in that: A first conductive groove (49) is provided on one side of the two first conductive members (44) that is away from each other, and the two first conductive grooves (49) respectively correspond to the positions of the two first conductive members (47). A second conductive groove (418) is provided on the bottom of the two second conductive members (45), and the two second conductive grooves (418) respectively correspond to the positions of the two second conductive members (416).

7. The electrical stimulation assembly according to claim 6, characterized in that: A limiting groove (419) is provided inside the iron core (42), and a limiting member (420) is slidably connected inside the limiting groove (419). The limiting member (420) passes through the bottom end of the iron core (42) and is fixedly connected to a magnet member (421). The magnet member (421) is fixedly connected to the outer walls of the two conductive columns (412). When current passes through the coil (43), the iron core (42) can generate magnetic force, which can push the magnet member (421) to move in a direction away from the iron core (42).

8. The electrical stimulation assembly according to claim 7, characterized in that: The outer wall of each conductive column (412) is provided with a guide groove (424), and the inner side of each limiting ring (411) is fixedly connected with a guide member (425) slidably connected to the corresponding guide groove (424). When the magnet member (421) drives the two conductive columns (412) to move, the two guide members (425) can move inside the corresponding guide groove (424), so that the two corresponding conductive columns (412) can rotate synchronously.

9. The electrical stimulation assembly according to claim 8, characterized in that: A partition plate (422) is fixedly connected to the top of the connection between the magnet member (421) and the two conductive columns (412); the two partition plates (422) are respectively arranged between two adjacent first conductive members (44) and second conductive members (45); a connection groove (423) is provided inside each of the partition plates (422); the two first conductive members (47) can move inside the corresponding connection groove (423); an extrusion member (426) fixedly connected to the partition plate (422) is provided inside each of the connection grooves (423); when the magnet member (421) moves in a direction away from the iron core (42), the extrusion member (426) can contact the corresponding first conductive member (47) and can extrude the first conductive member (47) to move in a direction away from the first conductive member (44).

10. The electrical stimulation assembly according to claim 9, characterized in that: The bottoms of the two conductive posts (412) are fixedly connected to the first electrode (31) and the second electrode (32), respectively. When the two conductive posts (412) are energized, they can supply power to the first electrode (31) and the second electrode (32). The two second conductive members (45) are electrically connected to the positive and negative output ports of the electrical stimulator (2) via cables (5).

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