PH valve and chromatographic equipment

Abstract

When the PH valve is in a detection position, the first end of a first liquid path is communicated with an outlet of an In connecting part, and the second end of the first liquid path is communicated with a second PH valve port; the first end of the second liquid path is communicated with the first PH valve port, and the second end of the second liquid path is communicated with an inlet of the Out connecting part; and at the bypass position, two ends of the third liquid path are respectively communicated with the outlet of the In connecting part and the inlet of the Out connecting part. According to the scheme, the defect that an existing system cannot be automatically switched and connected into a sensor can be overcome, and random switching of different external connection components can be achieved through one PH valve. According to the scheme, unnecessary operation in the experiment process is reduced, the analysis and preparation efficiency is improved, and meanwhile the cost is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of chromatography equipment, in particular to a PH valve and chromatography equipment. Background Art

[0002] Chromatographic equipment experiments use the differences in the physical and chemical properties of the components in a mixture to separate substances through different distributions in the chromatographic column and thus different flow rates. When using chromatographic equipment to analyze samples, different sensors and components are required to detect and analyze sample data, and due to the special properties of sensors (they cannot withstand high pressure and are consumables), there is a need to skip the sensor flow path when operating the system.

[0003] However, when the existing chromatography equipment on the market is connected to a chromatography column for experiments, especially when the system needs to be cleaned after the experiment, the sensor can only be short-circuited manually and the sensor must be removed and placed in a protective liquid. The operation is very cumbersome and easily damages the sensor and wastes experimental time.

[0004] Therefore, how to solve the problem of not being able to selectively connect the pH sensor and the back pressure valve during the experiment and how to solve the problem of not being able to quickly calibrate the sensor during the experiment have become important technical issues that need to be urgently solved by technical personnel in this field. Summary of the invention

[0005] In view of this, the present invention provides a PH valve, which can solve the defect that the existing system cannot automatically switch the connected sensor, and can realize arbitrary switching of different external components by using a PH valve.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A PH valve, used in chromatography equipment, comprises: a fixed part and a moving part;

[0008] The fixing member is provided with a PH valve inlet, a PH valve outlet, a first PH valve port and a second PH valve port;

[0009] The inlet of the In connection part is used to connect the upstream liquid path of the PH valve in the chromatography device, and the outlet of the Out connection part is used to connect the downstream liquid path of the PH valve in the chromatography device;

[0010] The moving part is provided with a first liquid path, a second liquid path and a third liquid path;

[0011] The moving part can move relative to the fixed part to a detection position and a bypass position;

[0012] In the detection position, the first end of the first liquid path is connected to the outlet of the In connection part, and the second end of the first liquid path is connected to the second PH valve port; the first end of the second liquid path is connected to the first PH valve port, and the second end of the second liquid path is connected to the inlet of the Out connection part;

[0013] When in the bypass position, both ends of the third fluid path are connected to the outlet of the In connection part and the inlet of the Out connection part respectively.

[0014] In some embodiments, the fixed member and the moving member are coaxially arranged, and the moving member can rotate relative to the fixed member to a detection position and a bypass position.

[0015] In some embodiments, the fixed part and the movable part respectively have a first inner surface and a second inner surface relative to each other, the first inner surface is provided with a PH valve inlet, a PH valve outlet, a first PH valve port and a second PH valve port, and the second inner surface is provided with a first liquid path, a second liquid path and a third liquid path.

[0016] In some embodiments, the inlet of the In connection portion and the inlet of the Out connection portion are respectively disposed on the outer surface of the fixing member.

[0017] In some embodiments, the fixing member is further provided with an FrR port and a ToR port;

[0018] The moving part is also provided with a fourth fluid circuit;

[0019] The moving part can also move relative to the fixed part to the back pressure valve and flow limiting PH position;

[0020] In the reverse pressure position, the first end of the fourth liquid path is connected to the outlet of the In connection part, and the second end of the fourth liquid path is connected to the ToR port; the two ends of the third liquid path are connected to the FrR port and the inlet of the Out connection part respectively;

[0021] When passing the back pressure valve and then the PH sensor position, the second end of the fourth liquid circuit is connected to the outlet of the In connection part, and the first end of the fourth liquid circuit is connected to the ToR port; the first end of the second liquid circuit is connected to the FrR port, and the second end of the second liquid circuit is connected to the second PH valve port; the middle part of the first liquid circuit is connected to the first PH valve port, and the second end of the first liquid circuit is connected to the inlet of the Out connection part.

[0022] In some embodiments, the fixing member is further provided with a W3 port and a Cal port;

[0023] The moving part is also provided with a fifth liquid path and a sixth liquid path;

[0024] The moving part can also move to a calibration position relative to the fixed part;

[0025] In the calibration position, two ends of the fifth liquid path are connected to the Cal port and the first PH valve port respectively, and two ends of the sixth liquid path are connected to the second PH valve port and the W3 port respectively.

[0026] In some embodiments, the fourth fluid path is arranged along the radial direction of the moving member and is symmetrical about the rotation center of the moving member;

[0027] The first liquid path and the second liquid path are located on one side of the fourth liquid path, and the third liquid path, the fifth liquid path and the sixth liquid path are located on the other side of the fourth liquid path.

[0028] In some embodiments, the distances from the PH valve inlet, the PH valve outlet, the FrR port, the second PH valve port, and the ToR port to the center of the fixing member are all R;

[0029] The distances from the first PH valve port, the second PH valve port and the Cal port to the center of the fixing member are all r, and r<R;

[0030] The distances between the outer edges of the first liquid path, the second liquid path, the third liquid path, the fourth liquid path, and the sixth liquid path and the center of the moving part are also R.

[0031] In some embodiments, the PH valve inlet, the PH valve outlet, the FrR port, the second PH valve port and the ToR port are arranged at equal intervals along the circumferential direction.

[0032] A chromatography device comprises the pH valve as described above.

[0033] It can be seen from the above technical solutions that the PH valve provided by the present invention can solve the defect that the existing system cannot automatically switch the connected sensor, and can realize the arbitrary switching of different external components by using a PH valve. This solution reduces unnecessary operations in the experimental process, improves the efficiency of analysis and preparation, and also reduces costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1 A schematic diagram of the structure of a PH valve provided in an embodiment of the present invention;

[0036] Figure 2 A schematic diagram of the structure of a PH valve provided in an embodiment of the present invention;

[0037] Figure 3a A schematic diagram of the structure of a pH sensor provided by an embodiment of the present invention;

[0038] Figure 3b A schematic diagram of the bypass structure provided by an embodiment of the present invention;

[0039] Figure 3c A schematic diagram of the structure of a flow limiter / back pressure valve provided in an embodiment of the present invention;

[0040] Figure 3d A schematic diagram of the structure of a current limiter and a pH sensor provided in an embodiment of the present invention;

[0041] Figure 3e A schematic diagram of the structure of pH calibration provided by an embodiment of the present invention;

[0042] Figure 4 A schematic diagram of the structure of a fixing member provided in an embodiment of the present invention;

[0043] Figure 5 A schematic diagram of the structure of a PH valve provided by an embodiment of the present invention;

[0044] Figure 6 A schematic diagram of the structure of another side of the PH valve provided by an embodiment of the present invention;

[0045] Figure 7 Another structural schematic diagram of a flow limiter / back pressure valve provided in an embodiment of the invention;

[0046] Figure 8 A schematic diagram of the structure of a moving part provided by an embodiment of the present invention;

[0047] Fig. 9 Another schematic diagram of the structure of the moving part provided by the embodiment of the present invention;

[0048] Fig.10 A schematic diagram of the three-dimensional structure of a moving part provided in an embodiment of the present invention.

[0049] Among them, 10 is a fixed part, and 20 is a moving part;

[0050] 11 is the PH valve inlet, 12 is the PH valve outlet, 13 is the first PH valve port, 14 is the second PH valve port, 15 is the FrR port, 16 is the W3 port, 17 is the Cal port, and 18 is the ToR port;

[0051] 21 is a first liquid path, 22 is a second liquid path, 23 is a third liquid path, 24 is a fourth liquid path, 25 is a fifth liquid path, and 26 is a sixth liquid path;

[0052] 31 is an In connection portion, and 32 is an Out connection portion. DETAILED DESCRIPTION

[0053] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0054] The PH valve provided in the embodiment of the present invention is used in a chromatography device, and includes: a fixed part 10 and a moving part 20; wherein the fixed part 10 is provided with a PH valve inlet 11, a PH valve outlet 12, a first PH valve port 13, a second PH valve port 14, an In connection part and an Out connection part; the In connection part is an input flow path of the front-stage system, and the Out connection part is an output flow path interface; the inlet of the In connection part is used to connect the upstream liquid path of the PH valve in the chromatography device, and the outlet of the Out connection part is used to connect the downstream liquid path of the PH valve in the chromatography device; the moving part 20 is provided with a first liquid path 21, a second liquid path 22 and a third liquid path 23; the moving part 20 can move to a detection position and a bypass position relative to the fixed part 10;

[0055] In the detection position, the first end of the first liquid path 21 is connected to the outlet of the In connection part, and the second end of the first liquid path 21 is connected to the second PH valve port 14; the first end of the second liquid path 22 is connected to the first PH valve port 13, and the second end of the second liquid path 22 is connected to the inlet of the Out connection part; Figure 3a A schematic diagram of the structure of the pH sensor provided in an embodiment of the present invention, wherein the upper right corner is the location for installing the pH sensor; liquid passes through the pH sensor from the upstream liquid path of the pH valve in the chromatography device through the In connection part, and then flows to the downstream liquid path of the pH valve in the chromatography device through the Out connection part;

[0056] When in the bypass position, the two ends of the third liquid path 23 are connected to the outlet of the In connection part and the inlet of the Out connection part respectively; Figure 3b A schematic diagram of the bypass structure provided by an embodiment of the present invention; liquid flows from the upstream liquid path of the PH valve in the chromatography device through the In connection part and directly flows from the Out connection part to the downstream liquid path of the PH valve in the chromatography device.

[0057] It can be seen from the above technical solutions that the PH valve provided in the embodiment of the present invention can solve the defect that the existing system cannot automatically switch the connected sensor, and can realize the arbitrary switching of different external components by using a PH valve. This solution reduces unnecessary operations in the experimental process, improves the efficiency of analysis and preparation, and also reduces costs.

[0058] In some embodiments of the present solution, the fixed member 10 and the moving member 20 are coaxially arranged, and the moving member 20 can rotate relative to the fixed member 10 to a detection position and a bypass position, and its structure facilitates state switching.

[0059] In some embodiments of the present invention, the fixed member 10 and the moving member 20 respectively have a first inner surface and a second inner surface opposite to each other, and the first inner surface is provided with a PH valve inlet 11, a PH valve outlet 12, a first PH valve port 13 and a second PH valve port 14. Figure 4 The second inner surface is provided with a first liquid path 21, a second liquid path 22 and a third liquid path 23, and its structure can refer to Figure 8 shown.

[0060] In some embodiments of the present solution, the inlet of the In connection portion and the inlet of the Out connection portion are respectively arranged on the outer surface of the fixing member 10 .

[0061] In some embodiments of the present invention, the fixing member 10 is further provided with an FrR port 15 and a ToR port 18, the structure of which can be referred to as Figure 4 As shown;

[0062] The moving part 20 is also provided with a fourth fluid path 24, the structure of which can be referred to Figure 8 As shown;

[0063] The moving part 20 can also move relative to the fixed part 10 to the back pressure valve and flow limiting PH position;

[0064] In the reverse pressure position, the first end of the fourth liquid path 24 is connected to the outlet of the In connection part, and the second end of the fourth liquid path 24 is connected to the ToR port 18; the two ends of the third liquid path 23 are connected to the FrR port 15 and the inlet of the Out connection part respectively; Figure 3c A schematic diagram of the structure of the flow restrictor / back pressure valve provided in an embodiment of the present invention; liquid enters the ToR port 18 from the upstream liquid path of the PH valve in the chromatography device through the In connection part, and then enters the FrR port 15 through the back pressure valve and leaves the downstream liquid path of the PH valve in the chromatography device through the Out connection part;

[0065] When passing through the back pressure valve and then the PH sensor position, the second end of the fourth liquid path 24 is connected to the outlet of the In connection part, and the first end of the fourth liquid path 24 is connected to the ToR port 18; the first end of the second liquid path 22 is connected to the FrR port 15, and the second end of the second liquid path 22 is connected to the second PH valve port 14; the middle part of the first liquid path 21 is connected to the first PH valve port 13, and the second end of the first liquid path 21 is connected to the inlet of the Out connection part; Figure 3d This is a schematic diagram of the structure of the embodiment of the present invention that passes through the flow limiter and then passes through the pH sensor; the liquid enters the ToR port 18 from the upstream liquid path of the pH valve in the chromatography device through the In connection part, then enters the FrR port 15 through the back pressure valve to pass through the pH sensor, and finally flows from the Out connection part to the downstream liquid path of the pH valve in the chromatography device. Through the above structural design, this solution can also realize the rapid switching of the back pressure function and the current limiting pH function.

[0066] In some embodiments of the present invention, the fixing member 10 is further provided with a W3 port 16 and a Cal port 17, the structure of which can be referred to as Figure 4 As shown;

[0067] The moving member 20 is also provided with a fifth liquid path 25 and a sixth liquid path 26;

[0068] The moving member 20 can also move relative to the fixed member 10 to a calibration position;

[0069] When in the calibration position, the two ends of the fifth liquid path 25 are connected to the Cal port 17 and the first PH valve port 13, respectively, and the two ends of the sixth liquid path 26 are connected to the second PH valve port 14 and the W3 port 16, respectively; Figure 3e The schematic diagram of the structure of the pH calibration provided by the embodiment of the present invention; the liquid enters from the upstream liquid path of the pH valve in the chromatography device through the Cal port 17 and flows to the downstream liquid path of the pH valve in the chromatography device through the W3 port 16. Through the above structural design, this solution can also realize the rapid switching of the calibration function.

[0070] In some embodiments of the present invention, the fourth fluid path 24 is arranged along the radial direction of the moving member 20 and is symmetrical about the rotation center of the moving member 20. Figure 4 As shown;

[0071] The first liquid channel 21 and the second liquid channel 22 are located on one side of the fourth liquid channel 24 , and the third liquid channel 23 , the fifth liquid channel 25 , and the sixth liquid channel 26 are located on the other side of the fourth liquid channel 24 .

[0072] In some embodiments of the present invention, the distances from the PH valve inlet 11, the PH valve outlet 12, the FrR port 15, the second PH valve port 16 and the ToR port 18 to the center of the fixing member 10 are all R. Figure 4 As shown;

[0073] The distances from the first PH valve port 13, the second PH valve port 14 and the Cal port 17 to the center of the fixing member 10 are all r, and r<R;

[0074] The distances between the outer edges of the first liquid path 21, the second liquid path 22, the third liquid path 23, the fourth liquid path 24 and the sixth liquid path 26 and the center of the moving part 20 are also R. Fig. 9 As shown, D=2R.

[0075] In some embodiments of the present solution, the PH valve inlet 11, the PH valve outlet 12, the FrR port 15, the second PH valve port 16 and the ToR port 18 are arranged at equal intervals along the circumferential direction.

[0076] like Figure 4-Figure 10As shown, the structure of the present invention includes a stator (i.e., a fixed part 10) and a rotor (i.e., a moving part 20). The rotor is tightly combined with the stator surface. During use, the rotor is connected to a specific hole on the stator by rotating; wherein there are a plurality of holes evenly divided by equal circular radius on the stator surface, namely, the PH valve inlet 11, the PH valve outlet 12, the FrR port 15, the second PH valve port 16 and the ToR port 18; wherein the hole at the center point is the inlet of the chromatography device to the PH valve, and the hole at a distance R5 from the circle is the outlet of the PH valve. The rotor is composed of a straight groove (i.e., the fourth liquid path 24) and a circular groove (i.e., the first liquid path 21) on the surface and an inner channel (i.e., the second liquid path 22, the third liquid path 23, the fifth liquid path 25 and the sixth liquid path 26). Each inlet and outlet of the PH valve are distributed on the stator surface at a distance R5 from the center of the circle. The function of arbitrarily connecting a sensor and a back pressure valve is realized by rotating the rotor to any hole on the radius R5.

[0077] The embodiment of the present invention further provides a chromatography device, including the PH valve as described above, which can be a protein purification instrument, a liquid chromatography instrument, etc., and does not require manual access to sensors, back pressure valves, and other operations.

[0078] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0079] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A PH valve for chromatography equipment, characterized in that: include: A fixed part (10) and a moving part (20); The fixing member (10) is provided with a PH valve inlet (11), a PH valve outlet (12), a first PH valve port (13), a second PH valve port (14), an In connection portion and an Out connection portion; The inlet of the In connection part is used to connect the upstream liquid path of the PH valve in the chromatography equipment, and the outlet of the Out connection part is used to connect the downstream liquid path of the PH valve in the chromatography equipment; The moving part (20) is provided with a first liquid path (21), a second liquid path (22) and a third liquid path (23); The moving part (20) can move relative to the fixed part (10) to a detection position and a bypass position; When in the detection position, the first end of the first liquid path (21) is connected to the outlet of the In connection part, and the second end of the first liquid path (21) is connected to the second PH valve port (14); the first end of the second liquid path (22) is connected to the first PH valve port (13), and the second end of the second liquid path (22) is connected to the inlet of the Out connection part; When in the bypass position, both ends of the third liquid path (23) are connected to the outlet of the In connection part and the inlet of the Out connection part respectively.

2. The PH valve according to claim 1, characterized in that: The fixed part (10) and the moving part (20) are coaxially arranged, and the moving part (20) can rotate relative to the fixed part (10) to a detection position and a bypass position.

3. The PH valve according to claim 2, characterized in that: The fixed part (10) and the movable part (20) respectively have a first inner surface and a second inner surface facing each other, the first inner surface is provided with a PH valve inlet (11), a PH valve outlet (12), a first PH valve port (13) and a second PH valve port (14), and the second inner surface is provided with a first liquid path (21), a second liquid path (22) and a third liquid path (23).

4. The PH valve according to claim 3, characterized in that: The inlet of the In connection part and the inlet of the Out connection part are respectively arranged on the outer surface of the fixing member (10).

5. The PH valve according to claim 3, characterized in that: The fixing member (10) is also provided with an FrR port (15) and a ToR port (18); The moving part (20) is also provided with a fourth liquid path (24); The moving part (20) can also move relative to the fixed part (10) to a back pressure valve and a flow limiting PH position; When in the reverse pressure position, the first end of the fourth liquid path (24) is connected to the outlet of the In connection part, and the second end of the fourth liquid path (24) is connected to the ToR port (18); the two ends of the third liquid path (23) are respectively connected to the FrR port (15) and the inlet of the Out connection part; When passing through the back pressure valve and then the pH sensor position, the second end of the fourth liquid path (24) is connected to the outlet of the In connection part, and the first end of the fourth liquid path (24) is connected to the ToR port (18); the first end of the second liquid path (22) is connected to the FrR port (15), and the second end of the second liquid path (22) is connected to the second pH valve port (14); the middle part of the first liquid path (21) is connected to the first pH valve port (13), and the second end of the first liquid path (21) is connected to the inlet of the Out connection part.

6. The PH valve according to claim 5, characterized in that: The fixing member (10) is also provided with a W3 port (16) and a Cal port (17); The moving part (20) is also provided with a fifth liquid path (25) and a sixth liquid path (26); The moving part (20) can also move relative to the fixed part (10) to a calibration position; When in the calibration position, the two ends of the fifth liquid path (25) are respectively connected to the Cal port (17) and the first PH valve port (13), and the two ends of the sixth liquid path (26) are respectively connected to the second PH valve port (14) and the W3 port (16).

7. The PH valve according to claim 6, characterized in that: The fourth fluid path (24) is arranged along the radial direction of the moving part (20) and is symmetrical about the rotation center of the moving part (20); The first liquid path (21) and the second liquid path (22) are located on one side of the fourth liquid path (24), and the third liquid path (23), the fifth liquid path (25) and the sixth liquid path (26) are located on the other side of the fourth liquid path (24).

8. The PH valve according to claim 7, characterized in that: The distances from the PH valve inlet (11), the PH valve outlet (12), the FrR port (15), the second PH valve port (16) and the ToR port (18) to the center of the fixing member (10) are all R; The distances from the first PH valve port (13), the second PH valve port (14) and the Cal port (17) to the center of the fixing member (10) are all r, and r<R; The distances between the outer edges of the first liquid path (21), the second liquid path (22), the third liquid path (23), the fourth liquid path (24) and the sixth liquid path (26) and the center of the moving part (20) are also R.

9. The PH valve according to claim 7, characterized in that: The PH valve inlet (11), the PH valve outlet (12), the FrR port (15), the second PH valve port (16) and the ToR port (18) are arranged at equal intervals along the circumferential direction.

10. A chromatography device, characterized in that: Comprising a PH valve as described in any one of claims 1-9.

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