Microcapsule artificial feed for babylonia in floating period and preparation method of microcapsule artificial feed

Abstract

The invention discloses microcapsule artificial feed for babylonia in a floating period and a preparation method of the microcapsule artificial feed. The microcapsule artificial feed for babylonia in the floating period is prepared from the following raw materials in percentage by mass: 25-40% of fish meal, 6-10% of shrimp meal, 4-8% of squids, 4-6% of oysters, 4-6% of whey protein powder, 2-5% of spray-dried animal blood cells, 5-7% of hermetia illucens larvae, 3-5% of fish oil, 1-3% of composite minerals, 4-6% of earthworms, 3-5% of beer yeast, 4-6% of sandworms and 1-3% of lecithin. The feed comprises 1-3% of EPA, 1-3% of DHA, 1-3% of a vitamin mixture and 1-3% of a microbial preparation. The microcapsule artificial feed is efficient, stable and good in palatability, and the metamorphosis rate and the growth speed of babylonia areolata larvae can be remarkably increased.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and particularly relates to a microcapsule artificial feed for babylonia snails in the planktonic stage and a preparation method thereof. Background Art

[0002] The traditional baits used in the planktonic period of Dongfeng snail mainly include biological algae, artificial algae powder, fish meal and related additives. However, some algae species are difficult to cultivate, age quickly and have difficulty in controlling density during the Dongfeng snail seedling period. In addition, some seedling enterprises and small workshops producing Dongfeng snail seedlings lack complete algae production facilities and equipment, making it difficult to carry out large-scale production, resulting in high costs, unstable output and quality, and difficulty in synchronizing with the time required for seedling feeding. At the same time, there are also hidden dangers of carrying pathogenic microorganisms and lack of highly polyunsaturated fatty acids (HUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). These factors directly hinder the stable and sustainable development of snail seedling production. In addition, artificial compound baits such as algae powder are produced and processed from natural baits. Due to problems in the current production process and other aspects, it is difficult to meet the requirements of Dongfeng snail larvae for bait specifications and nutritional components, so relevant adjuvants must be added. However, improper ratios often make it difficult for the larvae to eat. If the food is overfed or not eaten in time, it will decompose and release nitrogen and phosphorus, causing eutrophication of the water, breeding harmful bacteria, deteriorating water quality, and affecting the growth of the larvae, increasing the risk of larval disease. Therefore, the problem of biological bait replacement for the larvae of Babylonia square spot needs to be solved urgently. Summary of the invention

[0003] In view of this, the purpose of the present invention is a microcapsule artificial feed for the planktonic stage of Babylonia paniculata and a preparation method thereof. The microcapsule artificial feed of the present invention is efficient, stable, and has good palatability, and can significantly improve the metamorphosis rate and growth rate of Babylonia square spot larvae.

[0004] The specific technical solution adopted by the present invention is:

[0005] A microcapsule artificial feed for the planktonic stage of babylonica is prepared from the following raw materials in percentage by mass: 25-40% of fish meal, 6-10% of shrimp meal, 4-8% of squid, 4-6% of oyster, 4-6% of whey protein powder, 2-5% of hemoglobin powder, 5-7% of black soldier fly larvae, 3-5% of fish oil, 1-3% of composite minerals, 4-6% of earthworms, 3-5% of brewer's yeast, 4-6% of sandworms, 1-3% of lecithin, 1-3% of EPA, 1-3% of DHA, 1-3% of vitamin mixture and 1-3% of microbial preparation.

[0006] Preferably, the microcapsule artificial feed for the planktonic stage of Dongfeng snail is prepared from the following raw materials in percentage by mass: 40% fish meal, 8% shrimp meal, 6% squid, 5% oyster, 5% whey protein powder, 3% hemoglobin powder, 6% black soldier fly larvae, 4% fish oil, 2% complex minerals, 5% earthworms, 4% brewer's yeast, 5% sandworms, 2% lecithin, 1% EPA, 1% DHA, 1% vitamin mixture, and 2% microbial preparation.

[0007] Preferably, the content of the composite minerals is as follows: 35 g copper sulfate, 100 g ferrous sulfate, 35 g zinc sulfate, 25 g manganese sulfate, 0.8 g cobalt chloride, 0.5 g sodium selenite, 25 g potassium dihydrogen phosphate, 20 g sodium dihydrogen phosphate, and 642 g zeolite powder.

[0008] Preferably, the content of the vitamin mixture is as follows: 30g / kg vitamin A, 3g / kg vitamin D3, 60g / kg vitamin E, 6g / kg vitamin K3, 50g / kg vitamin B1, 4g / kg vitamin B2, 60g / kg vitamin B6, 6g / kg vitamin B12, 25g / kg niacin, 20g / kg calcium pantothenate, 0.3g / kg biotin, 220g / kg inositol, 6g / kg folic acid, and 359g / kg vitamin C.

[0009] Preferably, the content of the microbial preparation is as follows: 1.8 g / kg of lactobacillus, 3 g / kg of bacillus, and 1.6 g / kg of yeast.

[0010] Accordingly, the present invention also provides a method for preparing the above-mentioned microcapsule artificial feed for the planktonic stage of Babylonia snail, comprising the following steps:

[0011] (1) Fish meal, shrimp meal, squid, whey protein powder, hemoglobin powder, brewer's yeast are ultra-finely ground into powder;

[0012] (2) grinding oysters, black soldier fly larvae, earthworms, and sandworms into a colloid-like slurry A;

[0013] (3) The obtained fine powder is fully mixed with the remaining raw materials, and water is added to adjust the slurry and emulsified for 20 to 60 minutes to prepare a concentrated slurry B. The concentrated slurry A and the concentrated slurry B are mixed and homogenized to obtain a mixed concentrated slurry;

[0014] (4) The mixed slurry is sprayed, dried, sliced, sieved and cooled to obtain microcapsule artificial feed.

[0015] Preferably, step (1) is ultrafinely ground into powder of 180-400 mesh.

[0016] Preferably, the mass concentration of the concentrated slurry A is 25-65%; the mass concentration of the concentrated slurry B is 45-70%.

[0017] In the present invention:

[0018] As the main source of animal protein, fish meal has a balanced amino acid composition and is rich in vitamins, minerals and unknown growth factors, which can meet the nutritional needs of the baby snail during the breeding period.

[0019] Shrimp meal is rich in protein, low in fat, minerals and astaxanthin. The content of 6-10% can promote the growth of Dongfeng snails and enhance immunity.

[0020] Oysters are rich in high-quality protein, fat, minerals and multiple vitamins, and have a unique flavor and taste. They can promote the intake and digestion of Dongfeng snails and enhance the immunity of Dongfeng snails.

[0021] Whey protein contains a large amount of amino acids and protein, is easy to digest and absorb, and enhances immunity.

[0022] Hemoglobin powder is a high-quality protein source with a complete amino acid composition. It can effectively maintain the nutritional balance of feed and promote growth and development, immunity and nutritional balance.

[0023] Black soldier fly larvae are rich in nutrients, containing a large amount of medium-chain fatty acids and multiple unsaturated fatty acids, which improve survival rate and enhance resistance to stress.

[0024] Brewer's yeast mainly provides a protein source and also has the functions of enhancing appetite and aiding digestion.

[0025] Sandworms are rich in protein and amino acids, as well as unsaturated fatty acids and inorganic elements, which can enhance the immunity and survival rate of aquaculture materials.

[0026] Fish oil mainly provides energy and increases appetite.

[0027] Squid contains a large amount of nucleotides and amino acids, which can enhance the immunity and metabolic capacity of fish, promote the stress resistance and growth of Dongfeng snails, and also have food attractant properties.

[0028] Lecithin is supplemented for Babylonia snails to promote fat conversion and utilization.

[0029] The vitamin mixture mainly provides various vitamins to the fish body to improve the immunity and anti-stress ability of the conch.

[0030] The complex mineral element mixture provides various necessary trace elements for breeding materials and promotes metabolism.

[0031] Microbial preparations can promote the growth of beneficial bacteria, inhibit the production of pathogenic bacteria and harmful metabolites, enhance the nutrition of Dongfeng snails, and improve the immunity of Dongfeng snails.

[0032] Earthworms contain the amino acids needed for breeding materials, have strong feeding properties, improve conversion rates, and promote rapid growth.

[0033] EPA is an essential unsaturated fatty acid in the growth and development of fish, and plays an important role in the growth and development of fish.

[0034] DHA is an essential unsaturated fatty acid for the growth and development of fish. It plays an important role in the development of brain and optic nerves, as well as stress resistance and survival rate.

[0035] In summary, the oysters, black soldier fly larvae, vitamin mixtures and microbial preparations added in the present invention have synergistic effects, which can improve the anti-oxidation ability and immunity by regulating the oxidative stress of the baby snails, thereby improving the disease resistance of the baby snails; EPA and DHA can be stored in the body through metabolism, which has a promoting effect on growth; fish oil also contains rich DHA, thereby further promoting the storage of DHA in the baby snails. In addition, the proportion of carbohydrates, fats and proteins in the microcapsule artificial feed of the present invention is appropriate, the palatability is good, and the metamorphosis rate and growth rate of the baby baby snails can be significantly improved. DETAILED DESCRIPTION

[0036] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0037] Example 1

[0038] A microcapsule artificial feed for the planktonic stage of babylonica is prepared from the following raw materials in percentage by mass: 35% of fish meal, 6% of shrimp meal, 8% of squid, 6% of oyster, 4% of whey protein powder, 2% of hemoglobin powder, 5% of black soldier fly larvae, 3% of fish oil, 1% of composite minerals, 6% of earthworms, 4% of brewer's yeast, 5% of sandworms, 3% of lecithin, 3% of EPA, 3% of DHA, 3% of vitamin mixture and 3% of microbial preparation.

[0039] The content of the composite mineral is as follows: 35g copper sulfate, 100g ferrous sulfate, 35g zinc sulfate, 25g manganese sulfate, 08g cobalt chloride, 0.5g sodium selenite, 25g potassium dihydrogen phosphate, 20g sodium dihydrogen phosphate, and 642g zeolite powder.

[0040] The contents of the vitamin mixture are as follows: 30g / kg vitamin A, 3g / kg vitamin D3, 60g / kg vitamin E, 6g / kg vitamin K3, 50g / kg vitamin B1, 4g / kg vitamin B2, 60g / kg vitamin B6, 6g / kg vitamin B12, 25g / kg niacin, 20g / kg calcium pantothenate, 0.3g / kg biotin, 220g / kg inositol, 6g / kg folic acid, and 359g / kg vitamin C.

[0041] The contents of the microbial preparation are as follows: 1.8 g / kg of lactobacillus, 3 g / kg of bacillus, and 1.6 g / kg of yeast.

[0042] The preparation method thereof comprises the following steps:

[0043] (1) Fish meal, shrimp meal, squid, whey protein powder, hemoglobin powder, brewer's yeast are ultra-finely ground into 220 mesh powder;

[0044] (2) grinding oysters, black soldier fly larvae, earthworms, and sandworms into a colloid-milled concentrated slurry A, wherein the mass concentration of the concentrated slurry A is 30%;

[0045] (3) The obtained fine powder is fully mixed with the remaining raw materials, and water is added to adjust the slurry and emulsified for 40 minutes to prepare a concentrated slurry B, the mass concentration of which is 65%. The concentrated slurry A and the concentrated slurry B are mixed and homogenized to obtain a mixed concentrated slurry;

[0046] (4) The mixed slurry is sprayed, dried, sliced, sieved and cooled to obtain microcapsule artificial feed.

[0047] Example 2

[0048] The invention discloses an artificial microcapsule feed for the planktonic stage of babylonica, which is prepared from the following raw materials in percentage by mass: 40% of fish meal, 8% of shrimp meal, 6% of squid, 5% of oyster, 5% of whey protein powder, 3% of hemoglobin powder, 6% of black soldier fly larvae, 4% of fish oil, 2% of composite minerals, 5% of earthworms, 4% of brewer's yeast, 5% of sandworms, 2% of lecithin, 1% of EPA, 1% of DHA, 1% of vitamin mixture and 2% of microbial preparation.

[0049] The content of the composite mineral is as follows: 35g copper sulfate, 100g ferrous sulfate, 35g zinc sulfate, 25g manganese sulfate, 08g cobalt chloride, 0.5g sodium selenite, 25g potassium dihydrogen phosphate, 20g sodium dihydrogen phosphate, and 642g zeolite powder.

[0050] The contents of the vitamin mixture are as follows: 30g / kg vitamin A, 3g / kg vitamin D3, 60g / kg vitamin E, 6g / kg vitamin K3, 50g / kg vitamin B1, 4g / kg vitamin B2, 60g / kg vitamin B6, 6g / kg vitamin B12, 25g / kg niacin, 20g / kg calcium pantothenate, 0.3g / kg biotin, 220g / kg inositol, 6g / kg folic acid, and 359g / kg vitamin C.

[0051] The contents of the microbial preparation are as follows: 1.8 g / kg of lactobacillus, 3 g / kg of bacillus, and 1.6 g / kg of yeast.

[0052] The preparation method thereof comprises the following steps:

[0053] (1) Fish meal, shrimp meal, squid, whey protein powder, hemoglobin powder, brewer's yeast are ultra-finely ground into 300 mesh powder;

[0054] (2) grinding oysters, black soldier fly larvae, earthworms, and sandworms into a colloid-milled concentrated slurry A, wherein the mass concentration of the concentrated slurry A is 45%;

[0055] (3) The obtained fine powder is fully mixed with the remaining raw materials, and water is added to adjust the slurry and emulsified for 40 minutes to prepare a concentrated slurry B, wherein the mass concentration of the concentrated slurry B is 50%. The concentrated slurry A and the concentrated slurry B are mixed and homogenized to obtain a mixed concentrated slurry;

[0056] (4) The mixed slurry is sprayed, dried, sliced, sieved and cooled to obtain microcapsule artificial feed.

[0057] Example 3

[0058] The invention discloses an artificial microcapsule feed for the planktonic stage of babylonica, which is prepared from the following raw materials in percentage by mass: 37% of fish meal, 10% of shrimp meal, 4% of squid, 3% of oyster, 6% of whey protein powder, 5% of hemoglobin powder, 7% of black soldier fly larvae, 5% of fish oil, 3% of composite minerals, 5% of earthworms, 3% of brewer's yeast, 4% of sandworms, 1% of lecithin, 2% of EPA, 2% of DHA, 2% of vitamin mixture and 1% of microbial preparation.

[0059] The content of the composite mineral is as follows: 35g copper sulfate, 100g ferrous sulfate, 35g zinc sulfate, 25g manganese sulfate, 08g cobalt chloride, 0.5g sodium selenite, 25g potassium dihydrogen phosphate, 20g sodium dihydrogen phosphate, and 642g zeolite powder.

[0060] The contents of the vitamin mixture are as follows: 30g / kg vitamin A, 3g / kg vitamin D3, 60g / kg vitamin E, 6g / kg vitamin K3, 50g / kg vitamin B1, 4g / kg vitamin B2, 60g / kg vitamin B6, 6g / kg vitamin B12, 25g / kg niacin, 20g / kg calcium pantothenate, 0.3g / kg biotin, 220g / kg inositol, 6g / kg folic acid, and 359g / kg vitamin C.

[0061] The contents of the microbial preparation are as follows: 1.8 g / kg of lactobacillus, 3 g / kg of bacillus, and 1.6 g / kg of yeast.

[0062] The preparation method thereof comprises the following steps:

[0063] (1) Fish meal, shrimp meal, squid, whey protein powder, hemoglobin powder, brewer's yeast are ultra-finely ground into 280 mesh powder;

[0064] (2) grinding oysters, black soldier fly larvae, earthworms, and sandworms into a colloid-milled concentrated slurry A, wherein the mass concentration of the concentrated slurry A is 35%;

[0065] (3) The obtained fine powder is fully mixed with the remaining raw materials, and water is added to adjust the slurry and emulsified for 40 minutes to prepare a concentrated slurry B, the mass concentration of which is 55%. The concentrated slurry A and the concentrated slurry B are mixed and homogenized to obtain a mixed concentrated slurry;

[0066] (4) The mixed slurry is sprayed, dried, sliced, sieved and cooled to obtain microcapsule artificial feed.

[0067] Example 4

[0068] The invention discloses an artificial microcapsule feed for the planktonic stage of babylonica, which is prepared from the following raw materials in percentage by mass: 38% of fish meal, 6% of shrimp meal, 6% of squid, 9% of oyster, 6% of whey protein powder, 2% of hemoglobin powder, 6% of black soldier fly larvae, 4% of fish oil, 3% of composite minerals, 4% of earthworms, 5% of brewer's yeast, 6% of sandworms, 1% of lecithin, 1% of EPA, 1% of DHA, 1% of vitamin mixture and 1% of microbial preparation.

[0069] The content of the composite mineral is as follows: 35g copper sulfate, 100g ferrous sulfate, 35g zinc sulfate, 25g manganese sulfate, 08g cobalt chloride, 0.5g sodium selenite, 25g potassium dihydrogen phosphate, 20g sodium dihydrogen phosphate, and 642g zeolite powder.

[0070] The contents of the vitamin mixture are as follows: 30g / kg vitamin A, 3g / kg vitamin D3, 60g / kg vitamin E, 6g / kg vitamin K3, 50g / kg vitamin B1, 4g / kg vitamin B2, 60g / kg vitamin B6, 6g / kg vitamin B12, 25g / kg niacin, 20g / kg calcium pantothenate, 0.3g / kg biotin, 220g / kg inositol, 6g / kg folic acid, and 359g / kg vitamin C.

[0071] The contents of the microbial preparation are as follows: 1.8 g / kg of lactobacillus, 3 g / kg of bacillus, and 1.6 g / kg of yeast.

[0072] The preparation method thereof comprises the following steps:

[0073] (1) Fish meal, shrimp meal, squid, whey protein powder, hemoglobin powder, brewer's yeast are ultra-finely ground into 360 mesh powder;

[0074] (2) grinding oysters, black soldier fly larvae, earthworms, and sandworms into a colloid-milled concentrated slurry A, wherein the mass concentration of the concentrated slurry A is 55%;

[0075] (3) The obtained fine powder is fully mixed with the remaining raw materials, and water is added to adjust the slurry and emulsified for 40 minutes to prepare a concentrated slurry B, the mass concentration of which is 45%. The concentrated slurry A and the concentrated slurry B are mixed and homogenized to obtain a mixed concentrated slurry;

[0076] (4) The mixed slurry is sprayed, dried, sliced, sieved and cooled to obtain microcapsule artificial feed.

[0077] The application of the microcapsule artificial feed of the present invention in the planktonic period of the baby snails was tested. Four experimental groups and one control group were set up, with an average of three pools in each group, a total of 15 pools, and an area of ​​a single pool: 16m 2 , water volume 19.2m 3 ; Experimental group 1: microcapsule artificial feed for the planktonic period of baby snails in Example 2 + algae powder; Experimental group 2: microcapsule artificial feed for the planktonic period of baby snails in Example 2 + algae powder + Mudinas + shrimp yuan; Experimental group 3: microcapsule artificial feed for the planktonic period of baby snails in Example 2 + commercially available feed A; Experimental group 4: microcapsule artificial feed for the planktonic period of baby snails in Example 2; Control group: commercially available feed A + commercially available feed B. The density is high enough, the total amount of planktonic larvae is 3 million, and feeding begins. The specific feeding method is shown in the following table:

[0078]

[0079]

[0080] Note: After washing the seedlings, all experimental and control groups were fed with Dongwan brand Dongfeng snail compound feed 0# feed.

[0081] Each group was sampled and the test results were as follows:

[0082] Experimental Group 1: The initial seedling quantity was 3 million, the growth days were 15 days, and the average number of metamorphosed seeds falling to the ground was 870,000.

[0083] Experimental Group 2: The initial seedling quantity was 3 million, the growing days were 16 days, and the average number of metamorphosed seeds falling to the ground was 945,000.

[0084] Experimental Group 3: The initial seedling quantity was 3 million, the growing days were 17 days, and the average number of metamorphosed seeds falling to the ground was 1.214 million.

[0085] Experimental Group 4: The initial seedling quantity was 3 million, the growing days were 13 days, and the average number of metamorphosed seeds falling to the ground was 833,000.

[0086] Control group: initial seedling quantity was 3 million, growth days were 19 days, and the average number of metamorphosed seeds falling to the ground was 502,000.

[0087] It can be seen from this that microencapsulated artificial feed during the planktonic period of Babylonia snails can significantly increase the metamorphosis rate of Babylonia snails during the planktonic period.

[0088] Although the above embodiments have been described, those skilled in the art may make additional changes and modifications to these embodiments once they understand the basic creative concepts.

Claims

1. A microcapsule artificial feed for babylonia in the planktonic stage, characterized in that: The product is prepared from the following raw materials in percentage by mass: 25-40% fish meal, 6-10% shrimp meal, 4-8% squid, 4-6% oyster, 4-6% whey protein powder, 2-5% hemoglobin powder, 5-7% black soldier fly larvae, 3-5% fish oil, 1-3% complex minerals, 4-6% earthworms, 3-5% brewer's yeast, 4-6% sandworms, 1-3% lecithin, 1-3% EPA, 1-3% DHA, 1-3% vitamin mixture and 1-3% microbial preparation.

2. The microcapsule artificial feed for the planktonic stage of Babylonia snail according to claim 1, characterized in that: It is prepared from the following raw materials in percentage by mass: 40% fish meal, 8% shrimp meal, 6% squid, 5% oyster, 5% whey protein powder, 3% hemoglobin powder, 6% black soldier fly larvae, 4% fish oil, 2% complex minerals, 5% earthworms, 4% brewer's yeast, 5% sandworms, 2% lecithin, 1% EPA, 1% DHA, 1% vitamin mixture, and 2% microbial preparation.

3. The microcapsule artificial feed for the planktonic stage of Babylonia snail according to claim 1, characterized in that: The content of the composite mineral is as follows: 35g copper sulfate, 100g ferrous sulfate, 35g zinc sulfate, 25g manganese sulfate, 08g cobalt chloride, 0.5g sodium selenite, 25g potassium dihydrogen phosphate, 20g sodium dihydrogen phosphate, and 642g zeolite powder.

4. The microcapsule artificial feed for the planktonic stage of Babylonia snail according to claim 1, characterized in that: The contents of the vitamin mixture are as follows: 30g / kg vitamin A, 3g / kg vitamin D3, 60g / kg vitamin E, 6g / kg vitamin K3, 50g / kg vitamin B1, 4g / kg vitamin B2, 60g / kg vitamin B6, 6g / kg vitamin B12, 25g / kg niacin, 20g / kg calcium pantothenate, 0.3g / kg biotin, 220g / kg inositol, 6g / kg folic acid, and 359g / kg vitamin C.

5. The microcapsule artificial feed for the planktonic stage of Babylonia snail according to claim 1, characterized in that: The contents of the microbial preparation are as follows: 1.8 g / kg of lactobacillus, 3 g / kg of bacillus, and 1.6 g / kg of yeast.

6. The method for preparing the microcapsule artificial feed for the planktonic stage of Babylonia snails according to any one of claims 1 to 5, characterized in that: The steps include: (1) Fish meal, shrimp meal, squid, whey protein powder, hemoglobin powder, brewer's yeast are ultra-finely ground into powder; (2) grinding oysters, black soldier fly larvae, earthworms, and sandworms into a colloid-like slurry A; (3) The obtained fine powder is fully mixed with the remaining raw materials, and water is added to adjust the slurry and emulsified for 20 to 60 minutes to prepare a concentrated slurry B. The concentrated slurry A and the concentrated slurry B are mixed and homogenized to obtain a mixed concentrated slurry; (4) The mixed slurry is sprayed, dried, sliced, sieved and cooled to obtain microcapsule artificial feed.

7. The preparation method according to claim 6, characterized in that: Step (1) ultrafine grinding to 180-400 mesh powder.

8. The preparation method according to claim 6, characterized in that: The mass concentration of the concentrated slurry A is 25-65%; the mass concentration of the concentrated slurry B is 45-70%.

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