Ginseng extract microcapsule and application thereof in daily chemical products
By using ginseng extract microcapsules in hair care products, the problems of scalp damage and hair loss in the prior art have been solved, and safe and gentle oil control and anti-loss and blackening effects have been achieved, which has significantly improved the health status of hair.
Patent Information
- Application Number
- CN202411547006.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-01
- Publication Date
- 2024-12-31
AI Technical Summary
Commonly used chemicals in existing hair care products can cause scalp damage, hair loss and gray hair problems, and traditional medicines have adverse side effects and lack safe and mild solutions.
Use ginseng extract microcapsules, and wrap ginseng extract in microcapsules through preparation methods, including in shampoo, conditioner, essence or essence spray, and use ginseng saponins and polyphenols to promote scalp health. Reduce hair loss and gray hair and improve hair follicle activity.
Significantly reduce scalp damage, promote melanin synthesis, delay scalp aging, improve hair density and thickness, significantly improve hair health, and provide safe and gentle oil control, anti-deletion and blackening effects.
Smart Images
Figure CN119214975A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of daily chemical products, and specifically relates to a ginseng extract microcapsule and application thereof in daily chemical products. Background Art
[0002] Hair care products have become an indispensable part of people's lives. With the progress of modern society, people's living standards are gradually improving. But at the same time, people's life and mental stress are gradually increasing, and problems about hair and scalp care are also coming along. Long-term high-intensity work and unreasonable lifestyle lead to excessive scalp hair loss, graying hair and other problems. Excessive use of chemical shampoos on the market leads to a series of problems such as scalp damage, itching, dry hair, split ends, etc., which not only affects people's appearance, but also has a huge impact on people's self-confidence.
[0003] Androgenic alopecia (AGA, also known as seborrheic alopecia) is mainly caused by the conversion of testosterone (T), an androgen secreted by the human body, into dihydrotestosterone (DHT) under the action of 5-α reductase, and the binding rate of DHT to androgen receptor (AR) in hair follicle target cells is 10 times that of T to AR, which inhibits the growth and development of dermal papilla cells (DPC), which is the key signal for mammals to control the growth cycle of hair follicles, thereby causing hair follicles to end the growth phase prematurely and enter the resting phase, leading to AGA. Finasteride is the only oral drug approved by the US FDA to inhibit 5-α reductase, but it has adverse reactions such as male erectile dysfunction, sexual dysfunction, gynecomastia, and muscle growth disorders.
[0004] Gray hair is a group of localized white spots in the hair follicles, which can involve any hairy part of the body, including the scalp, eyebrows, eyelashes, or beard. Graying of hair may be due to a genetic defect in melanization, secondary to autoimmune destruction of pigment cells, or due to damage to the hair follicles. In addition, defects in melanin transfer caused by immune responses against cortical keratinocytes may contribute to the occurrence of gray hair. The histopathology of gray hair shows that melanin, melanocytes, or both are reduced or absent in the hair bulbs of infected hair follicles. In the biosynthesis of melanin in the skin, tyrosinase is the key enzyme that acts on dopa to form dopaquinone, which forms melanin after a spontaneous series of reactions.
[0005] In response to the problems of excessive oil secretion and gray hair, some toiletries with specific functions can effectively prevent or alleviate related problems and promote hair growth and blackening. However, so far, most toiletries usually add various preservatives, surfactants, etc. These raw materials bring certain effects to the products while also bringing certain adverse effects, thus causing hair damage. What's more, some products use a large amount of hormones, which bring great side effects to many male patients. Therefore, in recent years, safer and milder plant extracts have been widely studied so that they can be better applied in oil control, anti-hair loss, and gray hair blackening. Summary of the invention
[0006] The purpose of the present invention is to provide a ginseng extract microcapsule and application thereof in daily chemical products.
[0007] To achieve the purpose of the present invention, the technical solution adopted by the present invention is:
[0008] A method for preparing ginseng extract microcapsules comprises the following steps: adding ginseng extract to a wall material solution and stirring evenly, then adding an emulsifier and stirring again, and spray drying to obtain ginseng extract microcapsules; the core material of the microcapsules is ginseng extract; the method for preparing ginseng extract comprises: freeze-drying ginseng and grinding it into powder, adding the ginseng powder into deionized water, homogenizing to obtain a suspension, breaking the wall of the suspension, then adding a composite bacterial solution to ferment, adding a composite enzyme to the fermented product for enzymolysis, and extracting the enzymolysis solution using supercritical CO2 to obtain the ginseng extract.
[0009] Furthermore, the wall material is composed of the following components in parts by mass: 12 to 14 parts of chitosan, 14 to 16 parts of hydrogenated lecithin, 10 to 12 parts of xanthan gum, 1 to 1.5 parts of oligofructose, and 1 to 1.5 parts of glycerol.
[0010] Furthermore, the mass ratio of the core material: the wall material: the emulsifier is 7:4-6:0.1-0.2; the emulsifier is polyglycerol fatty acid ester, preferably polyglycerol-10 stearate.
[0011] Furthermore, the composite bacterial liquid includes Lactobacillus bulgaricus, Lactobacillus rhamnosus and Bifidobacterium longum.
[0012] Furthermore, the complex enzyme includes cellulase, amylase and ligninase; the mass ratio of the cellulase, amylase and ligninase is 6:2:3.
[0013] Furthermore, the supercritical CO2 extraction has a pressure of 30 to 35 Mbar, a temperature of 30 to 35°C, a CO2 flow rate of 5 to 8 mL / min, and an extraction time of 4 to 6 hours.
[0014] The present invention also provides a ginseng extract microcapsule, which is prepared by the above preparation method.
[0015] The present invention also provides application of the ginseng extract microcapsules in daily chemical products.
[0016] Furthermore, the daily chemical product is shampoo, conditioner, essence or essence spray.
[0017] Furthermore, the mass ratio of the ginseng extract microcapsules added to daily chemical products is 0.01-1%.
[0018] Beneficial Effects
[0019] Ginseng is a precious plant with a variety of medicinal values and effects that are beneficial to the human body. The ginseng extract of the present invention contains a variety of natural saponins and rich polysaccharides, polyphenols and other ingredients, and has the effects of immunomodulation, anti-cancer, anti-allergy, anti-oxidation, anti-inflammatory, promoting cell regeneration and improving sugar metabolism; among them, ginsenosides and polyphenols and other ingredients have obvious antioxidant effects, which can neutralize free radicals, reduce scalp damage, delay the aging process of the scalp, protect the health of the scalp, and reduce the degree of scalp damage.
[0020] The present invention adopts a microcapsule coating method to successfully embed the active ingredients of ginseng inside the microcapsule, thereby ensuring the stability of the active ingredients, extending the storage time, and better protecting the active ingredients such as ginsenosides, polysaccharides, and polyphenols, reducing their damage and waste, and making the dispersion more uniform.
[0021] In order to solve the problem of hair loss and gray hair, the present invention selects safer and milder plant extracts to be applied to the product. The ginseng extract obtained by the method of the present invention can significantly promote the concentration of melanin produced by melanocytes, promote the activity of tyrosinase, promote scalp blood circulation, enhance scalp vitality, open up the hair follicle channel, and after long-term use, it can increase the activity of tyrosinase in hair follicles and promote melanin synthesis to achieve the effect of black hair. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 This is a comparison chart of local hair density between Example 1 and Comparative Example 1. DETAILED DESCRIPTION
[0023] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the embodiments of the present invention will be described below.
[0024] Example 1
[0025] A method for preparing ginseng extract microcapsules, comprising the following steps: adding ginseng extract to a wall material solution and stirring evenly, then adding an emulsifier and stirring again, and spray drying to obtain ginseng extract microcapsules. The core material of the microcapsules is ginseng extract; the wall material is composed of the following components in parts by mass: 12 to 14 parts of chitosan, 14 to 16 parts of hydrogenated lecithin, 10 to 12 parts of xanthan gum, 1 to 1.5 parts of oligofructose, and 1 to 1.5 parts of glycerol; the emulsifier is polyglycerol fatty acid ester; the mass ratio of the core material: wall material: emulsifier is 7:4 to 6:0.1 to 0.2;
[0026] The preparation method of the ginseng extract comprises: freeze-drying ginseng and then grinding it into powder, adding the ginseng powder into deionized water, homogenizing to obtain a suspension, breaking the suspension, adding a composite bacterial liquid to ferment, adding a composite enzyme to the fermented product for enzymolysis, and extracting the enzymolysis solution with supercritical CO2 to obtain the ginseng extract.
[0027] The composite bacterial liquid includes Lactobacillus bulgaricus, Lactobacillus rhamnosus and Bifidobacterium longum; the composite enzyme includes cellulase, amylase and ligninase; the mass ratio of the cellulase, amylase and ligninase is 6:2:3. The supercritical CO2 extraction pressure is 30-35 Mbar, the temperature is 30-35°C, the CO2 flow rate is 5-8 mL / min, and the extraction time is 4-6 hours.
[0028] Ginseng extract microcapsules were added into shampoo at a mass ratio of 0.1%, and the formula of the shampoo was as follows:
[0029]
[0030]
[0031] Ginseng extract microcapsules were added to the hair mask at a mass ratio of 0.3%. The formula of the hair mask was as follows:
[0032]
[0033] Ginseng extract microcapsules were added to the essence at a mass ratio of 0.5%. The formula of the essence was as follows:
[0034]
[0035]
[0036] Ginseng extract microcapsules were added into the essence spray at a mass ratio of 0.5%. The formula of the essence spray was as follows:
[0037]
[0038]
[0039] Comparative Example 1
[0040] This comparative example provides an essence, which differs from comparative document 1 in that it does not contain the ginseng extract microcapsules described in Example 1, and the other conditions are exactly the same as those in Example 1.
[0041] Comparative Example 2
[0042] This comparative example provides an essence, which differs from the comparative document 1 in that the ginseng extract microcapsules do not contain a core material, and the other conditions are exactly the same as those in Example 1.
[0043] Comparative Example 3
[0044] This comparative example provides an essence, which differs from the comparative document 1 in that the ginseng extract microcapsules do not contain wall materials, and the other conditions are exactly the same as those in Example 1.
[0045] Comparative Example 4
[0046] This comparative example provides an essence, which differs from Comparative Document 1 in that the preparation method of the ginseng extract microcapsules does not include a freeze-drying step, and the other conditions are exactly the same as those in Example 1.
[0047] Comparative Example 5
[0048] This comparative example provides an essence, which differs from the comparative document 1 in that the preparation method of the ginseng extract microcapsules does not include a homogenization step, and the other conditions are exactly the same as those in Example 1.
[0049] Comparative Example 6
[0050] This comparative example provides an essence, which differs from the comparative document 1 in that the preparation method of the ginseng extract microcapsules does not include a wall-breaking step, and the other conditions are exactly the same as those in Example 1.
[0051] Comparative Example 7
[0052] This comparative example provides an essence, which differs from the comparative document 1 in that the preparation method of the ginseng extract microcapsules does not include a fermentation step, and the other conditions are exactly the same as those in Example 1.
[0053] Comparative Example 8
[0054] This comparative example provides an essence, which differs from the comparative document 1 in that the preparation method of the ginseng extract microcapsules does not include an enzymatic hydrolysis step, and the other conditions are exactly the same as those in Example 1.
[0055] Comparative Example 9
[0056] This comparative example provides an essence, which differs from comparative document 1 in that the preparation method of the ginseng extract microcapsules does not include a supercritical CO2 extraction step, and the other conditions are exactly the same as those in Example 1.
[0057] Example 2
[0058] Clinical Trial 1
[0059] Test samples: the essences of Example 1 and Comparative Example 1.
[0060] Subjects: 60 volunteers aged 20-60 years who met the inclusion criteria were divided into 2 groups, 30 in each group.
[0061] Test method: Test in accordance with the specific requirements of the "Technical Specification for Safety of Cosmetics" (2015 edition). Volunteer subjects were recruited according to the inclusion and exclusion criteria. Qualified subjects underwent a 2-week washout period. After the washout period, they were combed 60 times again. Only those with a hair loss count of more than 10 hairs could enter the formal test. The subjects were divided into two groups according to the stratified random method. The test group used the essence of Example 1, and the control group used the essence of Comparative Example 1. The subjects were evaluated for the basal hair value before using the essence, including hair loss count and hair density assessment, and recorded. According to the product instructions, the product was used continuously for 12 weeks, and the product was evaluated and tested again 4 weeks, 8 weeks, and 12 weeks after use. The test results of the test group and the control group, as well as the difference analysis results of the time points before and after are shown in the table below.
[0062] Test results of the experimental group and the control group
[0063]
[0064] Results of time difference analysis between the experimental group and the control group
[0065]
[0066] Note: NS means no significant difference when P≥0.05; S means significant difference when P<0.05.
[0067] After using the essence of Example 1, the difference in hair loss counts of the test group was significantly lower than that of the control group at each follow-up time point; the overall hair density of the test group did not decrease significantly at each follow-up time point; the difference in local hair density was significantly higher than that of the control group only at 12 weeks. In summary, the essence of Example 1 has an anti-hair loss effect.
[0068] Example 2
[0069] The mice were randomly divided into groups, and after ether anesthesia, a rosin-paraffin mixture hot melt with a weight ratio of 1:1 was applied to the back of each group of mice. After solidification, the mixture was uncovered, and the hair removal area was about 2 cm×2 cm (based on the smooth, uninjured, and hairless back of the mice). The essences prepared by the examples of the present invention and the comparative example methods were applied to the back of the mice twice a day, 0.2 mL / mouse each time, for a total of 30 days.
[0070] On the 31st day, the ten longest hairs in the hair removal area were taken and measured, and the average length was taken as the hair length. After the mice were killed, the fur in the hair removal area on the back was cut off, and the fur pieces were removed from the same area using a hole punch, and the body hair was shaved and weighed. The results are as follows:
[0071]
[0072] From the above results, it can be seen that the essence prepared by using the ginseng extract microcapsules of the present invention can effectively promote the growth of mouse hair, increase the hair length and weight of mice, and also promote the growth of hair follicles and increase the growth rate of hair follicles. In addition, the preparation method of the present invention also achieves a good extraction effect.
[0073] Example 3
[0074] Volunteers aged 25-45 were selected and randomly divided into groups. The volunteers were respectively treated with the essences of the embodiments and comparative examples of the present invention, and their hair was washed once a day, and then the essences were applied after washing.
[0075] Before the experiment and after using the serum for 3 months, 50 hairs were randomly selected from each volunteer, their average diameter was measured, and the hair diameter growth rate was calculated. The results are as follows:
[0076]
[0077] The above results show that long-term use of the product of the present invention can significantly improve the thickness of hair and make the hair stronger.
[0078] Example 5
[0079] Tyrosinase activation assay
[0080] A 0.2 mol / L phosphate buffer solution (pH 6.8) was used to prepare a 1 g / L L-dopa solution; a 0.2 mol / L phosphate buffer solution (pH 6.8) was used to prepare a 100 U / mL tyrosinase solution; a 0.2 mol / L phosphate buffer solution (pH 6.8) was used to prepare a 2 mg / mL test solution containing the essence of the embodiment or comparative example; 40 μL of the L-dopa solution, 40 μL of the test solution and / or 4 0 μL of tyrosinase solution was added to a 96-well plate, and then reacted in a 30°C water bath for 40 minutes; after the reaction was completed, the absorbance was tested at 490 nm, and each group of experiments was repeated 3 times. The absorbance of each group of experiments was the average of 3 tests to obtain absorbance C; referring to the above method, phosphate buffer was used instead of tyrosinase solution, and the absorbance was D; phosphate buffer was used instead of the test solution, and the absorbance was A; phosphate buffer was used instead of the test solution and tyrosinase solution, and the absorbance was B.
[0081] Tyrosinase activation rate (%) = [(CD) - (AB)] / (AB) * 100%
[0082] Among them, the higher the tyrosinase activation rate, the stronger the ability to promote melanin production, which means the better the hair darkening effect. See the table below for specific test data.
[0083]
[0084] The above test results show that the essence of Example 1 of the present invention has a significant effect on promoting tyrosinase activity and has a good effect of converting white hair into black hair.
Claims
1. A method for preparing ginseng extract microcapsules, characterized in that: The following steps are involved: Adding ginseng extract to the wall material solution and stirring evenly, then adding an emulsifier and stirring again, and spray drying to obtain ginseng extract microcapsules; The core material of the microcapsule is ginseng extract; The preparation method of the ginseng extract comprises: freeze-drying ginseng and then grinding it into powder, adding the ginseng powder into deionized water, homogenizing to obtain a suspension, breaking the suspension, adding a composite bacterial liquid to ferment, adding a composite enzyme to the fermented product for enzymolysis, and extracting the enzymolysis solution with supercritical CO2 to obtain the ginseng extract.
2. The method for preparing ginseng extract microcapsules according to claim 1, characterized in that: The wall material is composed of the following components in parts by weight: Composition: 12-14 parts of chitosan, 14-16 parts of hydrogenated lecithin, 10-12 parts of xanthan gum, 1-1.5 parts of oligofructose, and 1-1.5 parts of glycerol.
3. The method for preparing ginseng extract microcapsules according to claim 1, characterized in that: The mass ratio of the core material: the wall material: the emulsifier is 7:4-6:0.1-0.2; the emulsifier is polyglycerol fatty acid ester.
4. The method for preparing ginseng extract microcapsules according to claim 1, characterized in that: The composite bacterial liquid comprises Lactobacillus bulgaricus, Lactobacillus rhamnosus and Bifidobacterium longum.
5. The method for preparing ginseng extract microcapsules according to claim 1, characterized in that: The complex enzyme comprises cellulase, amylase and ligninase; the mass ratio of the cellulase, amylase and ligninase is 6:2:
3.
6. The method for preparing ginseng extract microcapsules according to claim 1, characterized in that: The pressure of supercritical CO2 extraction is 30-35 Mbar, the temperature is 30-35°C, the CO2 flow rate is 5-8 mL / min, and the extraction time is 4-6 hours.
7. A ginseng extract microcapsule, characterized in that: Prepared by the preparation method according to any one of claims 1 to 6.
8. Use of the ginseng extract microcapsules according to claim 7 in daily chemical products.
9. The use according to claim 8, characterized in that The daily chemical product is shampoo, conditioner, essence or essence spray.
10. The use according to claim 9, characterized in that: The mass ratio of the ginseng extract microcapsules added to daily chemical products is 0.01-1%.