Cell solution mixing mechanism and positive pressure sampling device

Abstract

The embodiment of the invention provides a cell solution uniform mixing mechanism and a positive pressure sampling device, the uniform mixing mechanism comprises a mounting seat, a test tube holder and a test tube, the test tube holder is mounted on the mounting seat, the test tube is arranged on the test tube holder, and the test tube is used for loading a cell solution to be uniformly mixed; a cavity is formed in the mounting seat, a vibration mechanism is arranged in the cavity, and the vibration mechanism is used for driving the test tube holder to vibrate in the direction perpendicular to the upper surface of the mounting seat; wherein the vibration mechanism can output different amplitude values. The cavity is formed in the mounting base, the vibration mechanism is arranged in the cavity, wiring can be reduced, the vibration mechanism can output different amplitude values according to the characteristics of different cell solutions, the uniform mixing effect is good, the cell survival rate is high, cell hanging on the wall is reduced, the structure is simple, operation is convenient and fast, and the stability and maintainability of equipment are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell solution processing, and in particular to a cell solution mixing mechanism and a positive pressure injection device. Background Art

[0002] In the treatment of cell solutions, it is often necessary to mix the cell solutions. At present, the mechanism for mixing the cell solutions generally adopts motor rotation mixing, which easily leads to uneven distribution of cells in the solution and makes cells adhere to the wall, thus reducing the survival rate of cells. Summary of the invention

[0003] In view of this, the embodiments of this specification provide a cell solution mixing mechanism and a positive pressure injection device. By opening a chamber in the mounting seat and arranging a vibration mechanism in the chamber, wiring can be reduced. The vibration mechanism can output different amplitude values ​​according to the characteristics of different cell solutions, so that the mixing effect is good and the cell survival rate is high.

[0004] The embodiments of this specification provide the following technical solutions: a cell solution mixing mechanism, including a mounting seat, a test tube holder and a test tube:

[0005] The test tube holder is mounted on the mounting seat, the test tube is placed on the test tube holder, and the test tube is used to load the cell solution to be mixed;

[0006] A chamber is provided in the mounting seat, and a vibration mechanism is provided in the chamber, and the vibration mechanism is used to drive the test tube holder to vibrate in a direction perpendicular to the upper surface of the mounting seat;

[0007] Wherein, the vibration mechanism can output different amplitude values.

[0008] Preferably, the vibration mechanism comprises piezoelectric ceramics, and the piezoelectric ceramics are used to drive the test tube holder to vibrate up and down, and when different voltages are input to the piezoelectric ceramics, the piezoelectric ceramics output different amplitude values.

[0009] Preferably, the vibration mechanism further comprises a mounting block and a mounting plate, the piezoelectric ceramic is mounted in the chamber via the mounting block, the piezoelectric ceramic is connected to the test tube holder via the mounting plate, and the mounting plate is in a floating state.

[0010] Preferably, the vibration mechanism also includes at least two groups of tension springs, the first ends of the tension springs are connected to the mounting plate, and the second ends of the tension springs are connected to the chamber, and under the action of the tension springs, the mounting plate always tends to move closer to the piezoelectric ceramic.

[0011] Preferably, the vibration mechanism further includes a plurality of mounting pins, and the second end of the tension spring is fixed in the chamber via the mounting pins.

[0012] Preferably, the tension springs are provided in two groups, and the two groups of tension springs are evenly distributed around the piezoelectric ceramic.

[0013] Preferably, the mounting seat is a fully sealed structure.

[0014] Preferably, the test tube holder is provided with an embedding groove, and the test tube is at least partially embedded in the embedding groove.

[0015] A positive pressure injection device comprises a feed chamber, an injection needle and a mixing mechanism as described in any one of the above items, wherein the feed chamber is mounted on the mounting seat, the mounting seat is at least partially inserted into the feed chamber, and the feed needle passes through the feed chamber and is inserted into the test tube.

[0016] Preferably, the positive pressure injection device also includes a plurality of sealing rings, which are arranged between the feed chamber and the mounting seat. When the mounting seat is inserted into the feed chamber for loading, the sealing rings are squeezed and deformed to ensure the sealing between the mounting seat and the feed chamber.

[0017] Compared with the prior art, the at least one technical solution adopted in the embodiments of this specification can achieve the following beneficial effects:

[0018] By opening a chamber in the mounting base and arranging a vibration mechanism in the chamber, wiring can be reduced. The vibration mechanism can output different amplitude values ​​according to the characteristics of different cell solutions, with good mixing effect, high cell survival rate, and reduced cell wall adhesion. It has a simple structure, is easy to operate, and improves the stability and maintainability of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 It is a structural schematic diagram of the cell solution mixing mechanism provided in the present application;

[0021] Figure 2 It is a schematic diagram of the internal structure of the chamber of the cell solution mixing mechanism provided in the present application;

[0022] Figure 3 It is a cross-sectional view of the positive pressure injection device provided in this application.

[0023] In the figure, 1. mounting seat; 2. test tube holder; 3. test tube; 4. chamber; 5. piezoelectric ceramic; 6. mounting plate; 7. mounting block; 8. tension spring; 9. mounting pin; 10. sealing ring; 11. feed chamber; 12. injection needle. DETAILED DESCRIPTION

[0024] The embodiments of the present application are described in detail below with reference to the accompanying drawings.

[0025] The following describes the implementation methods of the present application through specific examples, and those skilled in the art can easily understand other advantages and effects of the present application from the contents disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The present application can also be implemented or applied through other different specific implementation methods, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that, in the absence of conflict, the following embodiments and the features in the embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work belong to the scope of protection of the present application.

[0026] It should be noted that various aspects of the embodiments within the scope of the appended claims are described below. It should be apparent that the aspects described herein can be embodied in a wide variety of forms, and any specific structure and / or function described herein is merely illustrative. Based on the present application, it should be understood by those skilled in the art that an aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspect described herein can be used to implement the device and / or practice the method. In addition, other structures and / or functionalities other than one or more of the aspects described herein can be used to implement this device and / or practice this method.

[0027] It should also be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. The drawings only show components related to the present application rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0028] Additionally, in the following description, specific details are provided to facilitate a thorough understanding of the examples. However, it will be understood by those skilled in the art that the aspects described may be practiced without these specific details.

[0029] The traditional motor rotating mixing structure is used to mix the cell solution, which is prone to the following problems:

[0030] 1. The cells are unevenly distributed in the solution;

[0031] 2. The traditional motor rotation mixing structure results in low cell survival rate;

[0032] 3. The traditional motor rotating mixing structure easily causes cells to stick to the wall;

[0033] 4. Poor sealing, low injection pressure and slow injection speed.

[0034] After extensive and in-depth experiments, the inventor has designed a cell solution mixing mechanism and a positive pressure sample injection device.

[0035] The technical solutions provided by various embodiments of the present application are described below in conjunction with the accompanying drawings.

[0036] like Figure 1-Figure 2 As shown, a cell solution mixing mechanism includes a mounting seat 1, a test tube holder 2 and a test tube 3:

[0037] The test tube holder 2 is mounted on the mounting base 1, and the test tube 3 is placed on the test tube holder 2, and the test tube 3 is used to load the cell solution to be mixed;

[0038] A chamber 4 is provided in the mounting seat 1, and a vibration mechanism is provided in the chamber 4, and the vibration mechanism is used to drive the test tube holder 2 to vibrate in a direction perpendicular to the upper surface of the mounting seat 1;

[0039] Wherein, the vibration mechanism can output different amplitude values.

[0040] The cell solution to be mixed is held in the test tube 3, which is placed on the test tube holder 2. The test tube holder 2 is driven to vibrate up and down by the vibration mechanism, and the test tube 3 moves along with the test tube holder 2 to achieve a mixing operation on the cell solution in the test tube 3. The vibration mechanism can output different amplitude values ​​according to the characteristics of different cell solutions (such as viscosity, concentration, etc.), so as to achieve a good mixing effect, a high cell survival rate, and reduce cell wall adhesion.

[0041] It should be noted that the vibration mechanism is directly disposed in the chamber 4 of the mounting base 1, which can reduce the overall height of the mixing mechanism and reduce wiring, making the mixing mechanism more concise and beautiful.

[0042] By opening a chamber 4 in the mounting base 1 and arranging a vibration mechanism in the chamber 4, wiring can be reduced. The vibration mechanism can output different amplitude values ​​according to the characteristics of different cell solutions, with good mixing effect, high cell survival rate, reduced cell wall adhesion, simple structure, convenient operation, and improved stability and maintainability of the equipment.

[0043] like Figure 1-Figure 2As shown, in some embodiments, the vibration mechanism includes a piezoelectric ceramic 5, and the piezoelectric ceramic 5 is used to drive the test tube holder 2 to vibrate up and down. Different voltages are input to the piezoelectric ceramic 5, and the piezoelectric ceramic 5 outputs different amplitude values. By using the piezoelectric ceramic 5 to drive the test tube holder 2 to vibrate up and down, the piezoelectric ceramic 5 has the characteristics of large output and high resonance frequency. Even under load, it can still perform high-frequency excitation vibration above kilohertz. It is small in size, light in weight, has a long service life, can maintain stable vibration performance in harsh environments, is simple to drive, and usually only requires a simple voltage or current drive to achieve vibration. The vibration frequency of the piezoelectric ceramic 5 vibration plate can achieve a wide range of tunability by adjusting the driving voltage and frequency. By applying different input voltages to the piezoelectric ceramic 5, the piezoelectric ceramic 5 will output different amplitude values, which is suitable for mixing different cell solutions.

[0044] like Figure 1-Figure 2 As shown, in some embodiments, the vibration mechanism also includes a mounting block 7 and a mounting plate 6, the piezoelectric ceramic 5 is installed in the chamber 4 through the mounting block 7, the piezoelectric ceramic 5 is connected to the test tube holder 2 through the mounting plate 6, and the mounting plate 6 is in a floating state. By setting the mounting block 7, the mounting block 7 is specified to be installed on the bottom wall of the chamber 4, the piezoelectric ceramic 5 is installed on the mounting block 7, and the mounting plate 6 is set at the same time, the top of the piezoelectric ceramic 5 is connected to the bottom of the mounting plate 6, and the top of the mounting plate 6 is connected to the test tube holder 2. When the piezoelectric ceramic 5 vibrates, it drives the mounting plate 6 to vibrate together, and the mounting plate 6 drives the test tube 3 to vibrate through the test tube holder 2, thereby achieving the effect of mixing the cell solution in the test tube 3.

[0045] like Figure 1-Figure 2 As shown, in some embodiments, the vibration mechanism also includes at least two groups of tension springs 8, the first end of the tension spring 8 is connected to the mounting plate 6, and the second end of the tension spring 8 is connected to the chamber 4. Under the action of the tension spring 8, the mounting plate 6 always has a tendency to move closer to the piezoelectric ceramic 5. By setting the tension spring 8, the top end of the tension spring 8 is connected to the mounting plate 6, and the bottom end of the tension spring 8 is connected to the chamber 4. When the mounting plate 6 is in the initial position, the tension spring 8 is in a stretched state. The mounting plate 6 will always be in a tendency to stick to the upper end surface of the piezoelectric ceramic 5 under the limitation of the tension of the tension spring 8. When the piezoelectric ceramic 5 vibrates, under the action of the tension spring 8, the mounting plate 6 can maintain a relatively stable vibration, thereby ensuring the mixing effect of the cell solution in the test tube 3 and reducing cell wall adhesion.

[0046] It should be noted that in other embodiments, other elastic members, such as elastic strips, may be used instead of the tension spring 8 .

[0047] like Figure 2As shown, in some embodiments, the vibration mechanism also includes a plurality of mounting pins 9, the second end of the tension spring 8 is fixed in the chamber 4 through the mounting pins 9, and the bottom end of the tension spring 8 is fixed to the inner bottom wall of the chamber 4 through the mounting pins 9 to ensure the fixing effect of the tension spring 8.

[0048] like Figure 2 As shown, in some embodiments, the tension spring 8 is provided with two groups, and the two groups of tension springs 8 are evenly distributed around the piezoelectric ceramic 5. By providing two groups of tension springs 8 and the two groups of tension springs 8 are evenly distributed around the piezoelectric ceramic 5, it is possible to ensure that the mounting plate 6 is subjected to uniform force and ensure the stability of the mounting plate 6 when vibrating.

[0049] It should be noted that, in other embodiments, the number of the tension springs 8 may also be three groups, four groups, or other numbers, as long as the tension springs 8 are evenly distributed around the piezoelectric ceramic 5 .

[0050] like Figure 1-Figure 2 As shown, in some embodiments, the mounting base 1 is a fully sealed structure. By setting a vibration mechanism in the chamber 4, the mounting base 1 can be a fully sealed structure. There is no need to consider the sealing problem at the rotating shaft of the motor. The structure is simpler and maintenance is more convenient, thereby improving the overall sealing.

[0051] like Figure 1-Figure 2 As shown, in some embodiments, the test tube holder 2 is provided with an embedding groove, and the test tube 3 is at least partially embedded in the embedding groove. By providing the embedding groove on the test tube holder 2, the test tube 3 can be inserted into the embedding groove to avoid separation between the test tube 3 and the test tube holder 2 during the vibration of the test tube holder 2.

[0052] See also Figure 1-Figure 2 , the specific working process of the cell solution mixing mechanism will be described below:

[0053] The cell solution to be mixed is placed in the test tube 3, and the test tube 3 is placed in the embedded groove of the test tube holder 2. According to the characteristics of the cell solution (such as viscosity, concentration, etc.), a corresponding input voltage is applied to the piezoelectric ceramic 5, and the piezoelectric ceramic 5 outputs a corresponding amplitude value. When the piezoelectric ceramic 5 vibrates, it drives the mounting plate 6 to vibrate together. The mounting plate 6 drives the test tube 3 to vibrate through the test tube holder 2, so as to achieve the effect of mixing the cell solution in the test tube 3. When the mounting plate 6 vibrates with the piezoelectric ceramic 5, under the action of the tension spring 8, the mounting plate 6 can maintain a relatively stable vibration, thereby ensuring the effect of mixing the cell solution in the test tube 3 and reducing cell wall adhesion.

[0054] See also Figure 1-Figure 3Based on the same inventive concept, an embodiment of the present specification provides a positive pressure injection device, comprising a feed chamber 11, an injection needle 12 and a mixing mechanism as described in any one of the above items, wherein the feed chamber 11 is mounted on the mounting seat 1, the mounting seat 1 is at least partially inserted into the feed chamber 11, the feed needle passes through the feed chamber 11 and is inserted into the test tube 3, the top of the mounting seat 1 is inserted into the feed chamber 11, the feed needle is inserted into the bottom of the test tube 3, and the cell solution is injected through the feed needle.

[0055] It should be noted that a transparent window is provided on the feed cavity 11 to facilitate observation of the feeding situation of the feed needle.

[0056] During implementation, the positive pressure injection device also includes a plurality of sealing rings 10, and the plurality of sealing rings 10 are arranged between the feed chamber 11 and the mounting seat 1. When the mounting seat 1 is inserted into the feed chamber 11 for loading, the sealing ring 10 is squeezed and deformed to ensure the sealing between the mounting seat 1 and the feed chamber 11. By arranging the sealing ring 10 between the mounting seat 1 and the feed chamber 11, when the mounting seat 1 enters the feed chamber 11 for loading, the sealing ring is squeezed and deformed, which can ensure the sealing between the mounting seat 1 and the feed chamber 11, thereby increasing the injection pressure, increasing the injection speed, and improving the analysis and sorting efficiency.

[0057] It should be noted that in this embodiment, the sealing ring 10 is an O-ring, and the number of the sealing rings 10 is two. In other embodiments, the number of the sealing rings 10 can also be one, three, or other numbers, as long as the sealing between the mounting seat 1 and the feed chamber 11 can be achieved.

[0058] The same or similar parts between the various embodiments in this specification can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the method embodiment described later, since it corresponds to the system, the description is relatively simple, and the relevant parts can be referred to the partial description of the system embodiment.

[0059] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A cell solution mixing mechanism, characterized in that: Includes mount, tube holder and tube: The test tube holder is mounted on the mounting seat, the test tube is placed on the test tube holder, and the test tube is used to load the cell solution to be mixed; A chamber is provided in the mounting seat, and a vibration mechanism is provided in the chamber, and the vibration mechanism is used to drive the test tube holder to vibrate in a direction perpendicular to the upper surface of the mounting seat; Wherein, the vibration mechanism can output different amplitude values.

2. The cell solution mixing mechanism according to claim 1, characterized in that: The vibration mechanism includes piezoelectric ceramics, and the piezoelectric ceramics are used to drive the test tube holder to vibrate up and down. When different voltages are input to the piezoelectric ceramics, the piezoelectric ceramics output different amplitude values.

3. The cell solution mixing mechanism according to claim 2, characterized in that: The vibration mechanism also includes a mounting block and a mounting plate. The piezoelectric ceramic is mounted in the chamber via the mounting block. The piezoelectric ceramic is connected to the test tube holder via the mounting plate. The mounting plate is in a floating state.

4. The cell solution mixing mechanism according to claim 3, characterized in that: The vibration mechanism also includes at least two groups of tension springs, the first ends of the tension springs are connected to the mounting plate, and the second ends of the tension springs are connected to the chamber. Under the action of the tension springs, the mounting plate always tends to move closer to the piezoelectric ceramic.

5. The cell solution mixing mechanism according to claim 4, characterized in that: The vibration mechanism further comprises a plurality of mounting pins, and the second end of the tension spring is fixed in the chamber by the mounting pins.

6. The cell solution mixing mechanism according to claim 4, characterized in that: The tension springs are provided in two groups, and the two groups of tension springs are evenly distributed around the piezoelectric ceramics.

7. The cell solution mixing mechanism according to any one of claims 1 to 6, characterized in that: The mounting seat is a fully sealed structure.

8. The cell solution mixing mechanism according to any one of claims 1 to 6, characterized in that: The test tube holder is provided with an embedding groove, and the test tube is at least partially embedded in the embedding groove.

9. A positive pressure sample injection device, characterized in that: It comprises a feed chamber, an injection needle and a mixing mechanism as described in any one of claims 1 to 8, wherein the feed chamber is mounted on the mounting seat, the mounting seat is at least partially inserted into the feed chamber, and the feed needle passes through the feed chamber and is inserted into the test tube.

10. The positive pressure sample injection device according to claim 1, characterized in that: The positive pressure injection device also includes a plurality of sealing rings, which are arranged between the feed chamber and the mounting seat. When the mounting seat is inserted into the feed chamber for loading, the sealing rings are squeezed and deformed to ensure the sealing between the mounting seat and the feed chamber.

Images