Method and device for culturing integrated shortcut nitrification anaerobic ammonia oxidation granular sludge

By using high ammonia nitrogen wastewater and aerobic granular sludge in biological selection tanks, aeration tanks and precipitation selection tanks, the secretion of microbial extracellular polymers is solved, and the problem of additional substances required for anaerobic ammonia oxidized sludge granulation is achieved. Contamination-free and low-cost rapid anaerobic ammonia oxidation bacteria enrichment.

CN119219196AActive Publication Date: 2024-12-31BEIJING DRAINAGE GRP CO LTD

Patent Information

Application Number
CN202411575757.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-06
Publication Date
2024-12-31
Estimated Expiration
2044-11-06

AI Technical Summary

Technical Problem

In the prior art, the pelletized culture of anaerobic ammonia oxidized sludge requires additional injection of different kinds of substances, which may cause secondary pollution, and the operation is complex and costly.

Method used

Using the method of sequential overflow-connected biological selection tank, aeration tank, and precipitation selection tank, high ammonia nitrogen, low C/N wastewater and aerobic particle sludge, control ammonia nitrogen concentration and temperature, and promote the microbial extracellular polymer Secretion, maintain the form of aerobic granular sludge, quickly enrich anaerobic ammonia oxidizing bacteria, and realize the culture of integrated short-range nitrified anaerobic ammonia oxidizing granular sludge.

Benefits of technology

It realizes rapid culture without additional materials, reduces operational complexity and cost, avoids secondary contamination, and promotes efficient enrichment of anaerobic ammonia oxidizing bacteria through microbial niche replacement.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a method and a device for culturing integrated short-cut nitrification anaerobic ammonia oxidation granular sludge. The method comprises the following steps: sequentially connecting a biological selection tank, an aeration tank and a precipitation selection tank in an overflow manner; aerobic granular sludge and integrated short-cut nitrification anaerobic ammonia oxidation sludge are inoculated in the biological selection tank and the aeration tank, wherein the proportion of the integrated short-cut nitrification anaerobic ammonia oxidation sludge is 5%-10%. According to the method, aerobic granular sludge and short-cut nitrification anaerobic ammonia oxidation granular sludge have similar microbial layered structures, and rapid formation of the integrated short-cut nitrification anaerobic ammonia oxidation granular sludge based on microbial ecological niche conversion is promoted through ecological niche replacement of anaerobic ammonia oxidation bacteria. Part of effluent of the precipitation selection tank flows back, and the ascending velocity of liquid in the precipitation selection tank is increased to promote floc sludge elutriation and granular sludge enrichment. The method is convenient to operate and simple to control, can be applied to a continuous flow process, and is beneficial to quick start and stable operation of an integrated short-range anaerobic ammonia oxidation system.
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Description

Technical Field

[0001] The present invention belongs to the technical field of integrated short-range nitrification anaerobic ammonium oxidation granular sludge cultivation, and more specifically, relates to a method and device for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge. Background Art

[0002] The biological denitrification technology that combines anaerobic ammonium oxidizing bacteria (AnAOB) with ammonia oxidizing bacteria (AOB) in one reactor is called the integrated short-cut nitrification anaerobic ammonium oxidation (PN / A) process. AOB oxidizes a portion of the incoming ammonia nitrogen into nitrite nitrogen, and then AnAOB directly converts the remaining ammonia nitrogen and nitrite nitrogen into nitrogen gas. The PN / A method is a promising alternative to conventional nitrification / denitrification methods because it does not require organic matter and saves aeration energy consumption.

[0003] Despite the increasing importance of PN / A globally, its large-scale utilization remains difficult, with the enrichment of AnAOB remaining one of the major bottlenecks due to its low growth rate (0.065-0.33 day -1 ), and has obvious sensitivity to various environments. People have adopted a variety of strategies to enrich slow-growing AnAOB and enhance biomass cultivation, among which sludge granulation is a feasible and economical method. In recent years, a large number of researchers have carried out research on anaerobic ammonium oxidation sludge granulation. For example, Lin Xiangyu et al. invented "a method for rapid in-situ expansion of granular sludge", which uses the crystallization / precipitation formed by metal cations and phosphates, and the characteristics of metal cations and activated sludge with different charges, as the crystal nucleus and internal driving force of granular sludge agglomeration, respectively, to form granular sludge; Zhang Jing et al. invented "a method for rapid formation of anaerobic ammonium oxidation granular sludge", by adding 30C8-HSL quorum sensing signal molecules to promote anaerobic ammonium oxidation sludge granulation; Gong Hui et al. invented "a method for promoting rapid granulation of anaerobic ammonium oxidation bacteria using coagulants", by adding coagulants to control the protein ratio concentration and Zata potential value of extracellular polymers of microbial aggregates at different stages of granulation to achieve rapid granulation. In addition, inert carriers such as activated carbon, zeolite and bentonite can also be added, and the effect is greatly affected by the density of the carrier. Although the above methods promote the granulation of anaerobic ammonium oxidation sludge, they require the additional addition of different types of substances, which may cause secondary pollution, and the operation is complicated and the cost is high. Summary of the invention

[0004] The purpose of the present invention is to address the deficiencies in the prior art and provide a method and device for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge, so as to solve the problem that the existing anaerobic ammonium oxidation sludge granular culture requires additional addition of different types of substances, which may cause secondary pollution, and is complicated to operate and has high costs.

[0005] In order to achieve the above object, the present invention provides a method for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge, comprising:

[0006] The biological selection tank, aeration tank, and sedimentation selection tank are connected in sequence by overflow;

[0007] S1. Inoculate aerobic granular sludge and integrated short-cut nitrification anaerobic ammonium oxidation sludge with a total concentration of 5000-6000 mg / L in the biological selection tank and the aeration tank, wherein the proportion of the integrated short-cut nitrification anaerobic ammonium oxidation sludge is 5%-10%;

[0008] S2. Pump in wastewater with high ammonia nitrogen and low C / N, control the ammonia nitrogen concentration in the biological selection tank at 200-400 mg / L, and the ammonia nitrogen concentration in the aeration tank at 20-100 mg / L, and utilize the difference in pollutant concentrations between the biological selection tank and the aeration tank to promote the secretion of microbial extracellular polymers, maintain the aerobic granular sludge morphology, and efficiently retain anaerobic ammonia-oxidizing bacteria and rapidly enrich and grow;

[0009] S3. Control the nitrogen load of influent at 0.2kgN / m 3 / d, the temperature of the biological selection tank and the aeration tank is 30-35℃, the pH is 7.5-8.0, and the sedimentation selection tank returns the sludge to the biological selection tank;

[0010] S4. The total nitrogen removal rate is not less than 80% for 3 consecutive days. The water inlet is gradually increased until the inlet nitrogen load is 1.0 kgN / m 3 / d, part of the effluent from the sedimentation and selection tank is returned to the sedimentation and selection tank for washing the floc sludge and enriching the granular sludge.

[0011] Optionally, aerobic bacteria are distributed in the outer layer of the aerobic granular sludge, and anaerobic bacteria are distributed in the inner layer.

[0012] Optionally, the aerobic granular sludge has a particle size of 100-500 μm.

[0013] Optionally, part of the effluent from the sedimentation and selection tank is refluxed to achieve an upward flow rate of 1.0-10.0 m / h for the liquid in the sedimentation and selection tank, thereby promoting the washing of light flocculent sludge and enriching the granular sludge.

[0014] Optionally, the method further includes: utilizing a particle interception device to intercept the particles lost in the effluent and return them to the biological selection tank, thereby reducing the loss of granular sludge.

[0015] The present invention also provides a device for cultivating integrated short-range nitrification anaerobic ammonia oxidation granular sludge, comprising:

[0016] The biological selection tank, aeration tank, and sedimentation selection tank are connected in sequence by overflow;

[0017] The sludge outlet end of the sedimentation selection tank is connected to the biological selection tank through a sludge return pump;

[0018] The outlet end of the sedimentation and selection tank is provided with a particle interception device and a circulation pump, and part of the outlet water is refluxed to the inlet end of the sedimentation and selection tank through the circulation pump, and the lost particle sludge is intercepted by the particle interception device.

[0019] Optionally, a raw water tank is provided at the water inlet end of the biological selection tank, and aerobic granular sludge is directly added to the biological selection tank and the aeration tank.

[0020] Optionally, an aerator is provided in the aeration tank, and the aerator is connected to a blower via a gas flow meter.

[0021] Optionally, the precipitation selection tank comprises:

[0022] A pool body, wherein a mud collecting bucket is provided at the lower end of the pool body, and an overflow weir is provided at the upper end of the pool body;

[0023] The central tube is arranged in the middle of the tank body, a diffuser with a gradually expanding structure is arranged at the lower end, and an upper end is higher than the overflow weir and connected to the water outlet of the aeration tank.

[0024] Optionally, the particle retention device is connected to the biological selection tank.

[0025] The present invention provides a method for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge, which has the following beneficial effects:

[0026] This method is suitable for continuous flow process. It utilizes the similar microbial layered structure of aerobic granular sludge and short-term nitrification anaerobic ammonium oxidation granular sludge, with aerobic bacteria distributed in the outer layer and anaerobic bacteria distributed in the inner layer. The microbial niches are similar. The niche of anaerobic ammonium oxidizing bacteria can be replaced to promote the granulation of integrated short-term nitrification anaerobic ammonium oxidation granular sludge based on the transformation of microbial niches, thereby realizing the rapid cultivation of integrated short-term nitrification anaerobic ammonium oxidation granular sludge. At the same time, no additional materials need to be added and there is no secondary pollution.

[0027] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The above and other objects, features and advantages of the present invention will become more apparent through a more detailed description of exemplary embodiments of the present invention in conjunction with the accompanying drawings, wherein like reference numerals generally represent like components throughout the exemplary embodiments of the present invention.

[0029] Figure 1A schematic structural diagram of a device for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention is shown.

[0030] Figure 2 A schematic diagram of aerobic granular sludge in a method for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention is shown.

[0031] Figure 3 A schematic diagram of integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge cultivated according to a method for cultivating integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention is shown.

[0032] Figure 4 A graph showing changes in denitrification load during the startup phase of a method for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention is shown.

[0033] Figure 5 A graph showing the particle size variation of integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to a method for cultivating integrated short-cut nitrification and anaerobic ammonium oxidation granular sludge according to an embodiment of the present invention is shown.

[0034] Description of reference numerals:

[0035] 1.1, raw water tank; 2.1, biological selection tank; 2.2, aeration tank; 2.3, sludge return pump; 2.4, gas flow meter; 2.5, blower; 2.6, aerator; 3.1, sedimentation selection tank; 3.2, circulation pump; 3.3, particle retention device. DETAILED DESCRIPTION

[0036] The preferred embodiments of the present invention will be described in more detail below. Although the preferred embodiments of the present invention are described below, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0037] like Figure 1-5 As shown, a device for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge comprises:

[0038] The biological selection tank 2.1, the aeration tank 2.2, and the sedimentation selection tank 3.1 are connected in sequence by overflow;

[0039] S1. Inoculate aerobic granular sludge and integrated short-cut nitrification anaerobic ammonium oxidation sludge with a total concentration of 5000-6000 mg / L in the biological selection tank 2.1 and the aeration tank 2.2, wherein the proportion of the integrated short-cut nitrification anaerobic ammonium oxidation sludge is 5%-10%;

[0040] S2, pump in wastewater with high ammonia nitrogen and low C / N, control the ammonia nitrogen concentration of biological selection tank 3.1 to 200-400 mg / L, the ammonia nitrogen concentration of aeration tank 2.2 to 20-100 mg / L, and the DO to 0.3-1.0 mg / L, and utilize the difference in pollutant concentrations between the biological selection tank and the aeration tank to promote the secretion of microbial extracellular polymers, maintain the aerobic granular sludge morphology, and efficiently retain anaerobic ammonia-oxidizing bacteria and rapidly enrich and grow;

[0041] S3. Control the nitrogen load of influent at 0.2kgN / m 3 / d, the temperature of biological selection tank 2.1 and aeration tank 2.2 is 30-35°C, the pH is 7.5-8.0, and the sedimentation selection tank 3.1 returns the sludge to the biological selection tank 2.1;

[0042] S4. When the total nitrogen removal rate is not less than 80% for at least 3 consecutive days, gradually increase the water flow until the nitrogen load of the water reaches 1.0 kgN / m 3 / d, part of the effluent from the sedimentation and selection tank 3.1 is returned to the sedimentation and selection tank 3.1 for washing the flocculent sludge and enriching the granular sludge.

[0043] Specifically, the aerobic granular sludge used in this method is simple and easy to obtain, the granulation startup cost is low, no other substances are added, there is no secondary pollution, and the operation process is simple;

[0044] Among them, aerobic granular sludge has good settling properties and large biomass, and is an excellent biological carrier. When the integrated short-term nitrification anaerobic ammonium oxidation sludge is insufficient, aerobic granular sludge is introduced to enhance the retention of anaerobic ammonium oxidizing bacteria, which can quickly enrich the anaerobic ammonium oxidizing bacteria to start the reactor, and realize the rapid cultivation of a large amount of integrated short-term nitrification anaerobic ammonium oxidation sludge by using a small amount of integrated short-term nitrification anaerobic ammonium oxidation sludge as seeds.

[0045] The pollutant concentrations in the biological selection tank and the aeration tank are different, forming a concentration difference environment. The continuous flow causes the granular sludge to cycle alternately between a feast / starvation environment, promoting the secretion of microbial extracellular polymers and maintaining the granular morphology of aerobic granular sludge.

[0046] The sedimentation and selection tank adjusts the rising flow rate in the sedimentation and selection tank by adjusting the reflux flow rate, so as to achieve the effect of washing light flocs and rapidly enriching and growing the integrated short-range nitrification and anaerobic ammonia oxidation granular sludge.

[0047] In this embodiment, aerobic bacteria are distributed in the outer layer of the aerobic granular sludge, and anaerobic bacteria are distributed in the inner layer.

[0048] Specifically, the inoculated sludge is mainly aerobic granular sludge, which uses the advantages of good sedimentation and large biomass of aerobic granular sludge as a biological carrier to replace the internal anaerobic bacteria with AnAOB, thereby achieving efficient retention and enrichment of AnAOB. ​​Generally, it takes at least 3-6 months for AnAOB to form granular sludge by secreting extracellular proteins. This solution introduces aerobic granular sludge, and the aerobic granular sludge is transformed into red particles visible to the naked eye in just one month.

[0049] In this embodiment, the particle size of the aerobic granular sludge is 100-500 μm.

[0050] Specifically, choosing aerobic granular sludge of appropriate particle size is beneficial to the ecological position replacement of AnAOB, and avoids the situation where the particle size is too small and an effective anaerobic layer cannot be formed after replacement, affecting the activity of AnAOB, or the particle size is too large and the thickness increases, making internal replacement difficult.

[0051] In this embodiment, part of the effluent from the circulating sedimentation and selection tank 3.1 is returned to achieve an upward flow rate of 1.0-10.0 m / h in the sedimentation and selection tank 3.1, thereby promoting the washing of light flocculent sludge and enriching the granular sludge.

[0052] In this embodiment, it also includes: using the particle interception device 3.3 to intercept the lost particles in the effluent and return them to the biological selection tank 2.1, so as to reduce the loss of granular sludge.

[0053] The present invention also provides a device for cultivating integrated short-range nitrification anaerobic ammonia oxidation granular sludge, comprising:

[0054] The biological selection tank 2.1, the aeration tank 2.2, and the sedimentation selection tank 3.1 are connected in sequence by overflow;

[0055] The sludge outlet of the sedimentation selection tank 3.1 is connected to the biological selection tank 2.1 through the sludge return pump 2.3;

[0056] The outlet of the sedimentation and selection tank 3.1 is provided with a particle interception device 3.3 and a circulation pump 3.2. Part of the outlet water is refluxed to the inlet of the sedimentation and selection tank 3.1 through the circulation pump 3.2, and the lost particle sludge is intercepted by the particle interception device 3.3.

[0057] Specifically, aerobic granular sludge and short-term nitrification anaerobic ammonium oxidation granular sludge have similar microbial layered structures, with aerobic bacteria distributed in the outer layer and anaerobic bacteria distributed in the inner layer. The microbial niches are similar, and the niche replacement of AnAOB can promote the granulation of integrated short-term nitrification anaerobic ammonium oxidation granular sludge based on microbial niche transformation, with the preferred particle size being 100-500μm. When in use, aerobic granular sludge and a small amount of integrated short-term nitrification anaerobic ammonium oxidation sludge are inoculated in the biological selection tank 2.1. The aerobic granular sludge is used as a biological carrier to quickly enrich AnAOB to form integrated short-term nitrification anaerobic ammonium oxidation granular sludge by utilizing the similar microbial niches between the aerobic granular sludge and the good settling ability and large biomass of the aerobic granular sludge. The effluent circulation is utilized to increase the rising flow rate of the water flow in the sedimentation selection tank 3.2 to achieve the washing of the light floc sludge and promote the enrichment of the granular sludge. The effluent of the sedimentation selection tank 3.1 intercepts the granular sludge and returns it to the biological selection tank 2.1 to further cultivate the granular sludge.

[0058] Furthermore, the volume ratio of the biological selection tank 2.1 to the aeration tank 2.2 is 0.1-0.6; the water is evenly distributed at the bottom of the biological selection tank 2.1, and the height-to-diameter ratio is 4-6. When the height-to-diameter ratio requirement cannot be met in practice, multiple sets of parallel biological selection tanks 2.1 can be set; the sludge return pump 2.3 adopts a positive displacement pump such as a spiral centrifugal pump or a diaphragm pump that has little damage to particles.

[0059] In this embodiment, a raw water tank 1.1 is provided at the water inlet of the biological selection tank 2.1, and aerobic granular sludge is directly added to the biological selection tank 2.1 and the aeration tank 2.2.

[0060] Specifically, the water quality of the incoming water is pre-adjusted by the raw water tank 1.1.

[0061] In this embodiment, an aerator 2.6 is provided in the aeration tank 2.2, and the aerator 2.6 is connected to the blower 2.5 via a gas flow meter 2.4.

[0062] Specifically, the aeration volume is accurately controlled by the gas flow meter 2.4.

[0063] In this embodiment, the sedimentation selection tank 3.1 includes:

[0064] The pool body has a mud collecting bucket at the lower end and an overflow weir at the upper end;

[0065] The central tube is arranged in the middle of the tank body, a diffuser with a gradually expanding structure is arranged at the lower end, and an upper end is higher than the overflow weir and connected to the water outlet of the aeration tank 2.2.

[0066] Specifically, the bottom of the central tube of the sedimentation and selection tank 3.1 is 0.3-0.5m away from the tank bottom to impact the bottom sediment sludge and enhance the sludge selection effect.

[0067] In this embodiment, the particle interception device 3.3 is connected to the biological selection tank 2.1.

[0068] Specifically, the granular sludge is circulated through the granular retention device to prevent the loss of the granular sludge.

[0069] Example

[0070] The device for rapidly cultivating integrated short-range nitrification anaerobic ammonia oxidation granular sludge comprises: a raw water tank 1.1, a biological selection tank 2.1, an aeration tank 2.2, and a sedimentation selection tank 3.1; the raw water tank 1.1 is connected to the biological selection tank 2.1 through an inlet pump 1.2, and the mud-water mixture flowing out of the upper part of the biological selection tank 2.1 enters the aeration tank 2.2. The aeration tank 2.2 includes a blower 2.5, an aerator 2.6, and a gas flow meter 2.4. The aeration tank 2.2 is aerated by the blower 2.5. The aerator 2.6 is installed at the bottom of the tank and is connected to the blower 2.5 through an air pipeline. The gas volume is adjusted by the gas flow meter 2.4. The effluent from the aeration tank 2.2 enters the central cylinder of the sedimentation selection tank 3.1. The sedimentation selection tank 3.1 is connected to the biological selection tank 2.1 through a sludge return pump 2.3. A part of the effluent is connected to the outlet pipe of the aeration tank 2.2 through an effluent circulation pump 3.2 and enters the central cylinder of the sedimentation selection tank 3.1 to increase the rising flow rate of the sedimentation selection tank. The remaining part of the effluent enters the particle interception device 3.2, the particle interception returns to the aeration tank 2.2, and the effluent is discharged.

[0071] The bottom of the biological selection tank 2.1 is evenly distributed with water, and the height-to-diameter ratio is 5. A circular water outlet weir is provided on the top of the biological selection tank 2.1, and the mud-water mixture in the tank enters the aeration tank 2.2 through the water outlet weir. The volume ratio of the biological selection tank 2.1 to the aeration tank 2.2 is 0.3. The bottom of the central cylinder of the sedimentation selection tank 3.1 is 0.3m away from the bottom of the tank to impact the bottom sedimentation sludge and enhance the sludge selection effect; the sludge return pump 2.3 adopts a spiral centrifugal pump with little damage to particles. Online instruments and automatic control components can be configured to realize automatic control of the system.

[0072] The operating method of the device for rapidly cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge of the present invention comprises the following steps:

[0073] (1) Inoculation sludge: 90% aerobic granular sludge and 10% integrated anaerobic ammonium oxidation sludge were inoculated in the biological selection tank 2.1 and the aeration tank 2.2, with a total concentration of 6000 mg / L and a particle size of the aerobic granular sludge of 300 μm.

[0074] (2) Start-up phase: The raw water tank 1.1 contains high ammonia nitrogen and low C / N wastewater prepared by adding ammonium bicarbonate and sodium bicarbonate to the effluent from the primary sedimentation tank of urban sewage. The ammonia nitrogen concentration is 300-600 mg / L and the C / N is lower than 1.5. The inlet pump 1.2 is used to continuously pump wastewater into the biological selection tank 2.1, and the ammonia nitrogen concentration in the biological selection tank is controlled at 200-400 mg / L; the effluent from the biological selection tank 2.1 enters the aeration tank 2.2, and the ammonia nitrogen concentration in the aeration tank 2.2 is controlled at 20-100 mg / L. The difference in pollutant concentration between the biological selection tank 2.1 and the aeration tank 2.2 is used to promote the secretion of microbial extracellular polymers and maintain the particle morphology of aerobic granular sludge. The blower 2.5 of the aeration tank 2.2 is turned on to supply air to the aeration tank 2.2 through the aerator 2.6, and the gas volume of the aeration tank is controlled by the gas flow meter 2.4 to maintain the DO at 0.3-1.0 mg / L; the temperature of the biological selection tank 2.1 and the aeration tank 2.2 is controlled at 30-35°C, the pH at 7.5-8.0, and the nitrogen load of the influent at 0.2 kgN / m 3 / d, start the sludge return pump 2.3, control the sludge return ratio to 0.3-1.0, and the effluent from the sedimentation selection tank 3.1 enters the particle interception device 3.3; measure the effluent quality, and when the total nitrogen removal rate reaches 80% for 3 consecutive days, the reactor startup is completed.

[0075] (3) Rapid cultivation of integrated anaerobic ammonium oxidation granular sludge: Aerobic granular sludge and short-range nitrification anaerobic ammonium oxidation granular sludge have similar microbial layered structures, with aerobic bacteria distributed in the outer layer and anaerobic bacteria distributed in the inner layer. The microbial niches are similar and the cultivation of integrated anaerobic ammonium oxidation granular sludge can be accelerated through niche replacement of AnAOB. ​​By utilizing the different pollutant concentrations in the biological selection tank 2.1 and the aeration tank 2.2, a feast / starvation environment is maintained to promote the transformation of microbial niches and form integrated anaerobic ammonium oxidation granular sludge. Maintaining the difference in pollutant concentrations between the biological selection tank 2.1 and the aeration tank 2.2, adjust the influent flow rate and gradually increase the influent nitrogen load to 1.0 kgN / m 3 / d. At the same time, the effluent circulation pump 3.2 of the sedimentation and selection tank 3.1 is turned on. According to the growth of the sludge concentration in the aeration tank 2.2, the effluent circulation pump flow 3.2 and the rising flow rate of the sedimentation and selection tank 3.1 are gradually increased to wash the flocculent sludge and enrich and cultivate the granular sludge. The rising flow rate is adjusted in the range of 1.0-10.0m / h. The effluent enters the granular interception device 3.3 to achieve rapid enrichment and growth of the granular sludge.

[0076] Under the above conditions, after the reactor was operated for 30 days, it was found that the total nitrogen removal load was increased from 0.2 kgN / m 3 / d increased to 0.6kgN / m 3 / d, the color of aerobic granular sludge changed to obvious red, the particle size increased from 250μm to 310μm, and red particles visible to the naked eye were formed.

[0077] The embodiments of the present invention have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments.

Claims

1. A method for cultivating integrated short-term nitrification anaerobic ammonia oxidation granular sludge, characterized in that: include: The biological selection tank, aeration tank, and sedimentation selection tank are connected in sequence by overflow; S1. Inoculate aerobic granular sludge and integrated short-cut nitrification anaerobic ammonium oxidation sludge with a total concentration of 5000-6000 mg / L in the biological selection tank and the aeration tank, wherein the proportion of the integrated short-cut nitrification anaerobic ammonium oxidation sludge is 5%-10%; S2. Pump in wastewater with high ammonia nitrogen and low C / N, control the ammonia nitrogen concentration in the biological selection tank at 200-400 mg / L, and the ammonia nitrogen concentration in the aeration tank at 20-100 mg / L, and utilize the difference in pollutant concentrations between the biological selection tank and the aeration tank to promote the secretion of microbial extracellular polymers, maintain the aerobic granular sludge morphology, and efficiently retain anaerobic ammonia-oxidizing bacteria and rapidly enrich and grow; S3. Control the nitrogen load of influent at 0.2kgN / m 3 / d, the temperature of the biological selection tank and the aeration tank is 30-35℃, the pH is 7.5-8.0, and the sedimentation selection tank returns the sludge to the biological selection tank; S4. The total nitrogen removal rate is not less than 80% for 3 consecutive days. The water inlet is gradually increased until the inlet nitrogen load is 1.0 kgN / m 3 / d, part of the effluent from the sedimentation and selection tank is returned to the sedimentation and selection tank for washing the floc sludge and enriching the granular sludge.

2. The method for cultivating integrated short-cut nitrification anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that: The aerobic granular sludge has aerobic bacteria distributed in the outer layer and anaerobic bacteria distributed in the inner layer.

3. The method for cultivating integrated short-cut nitrification anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that: The particle size of the aerobic granular sludge is 100-500 μm.

4. The method for cultivating integrated short-cut nitrification anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that: The effluent from the sedimentation and selection tank is partially refluxed to achieve an upward flow rate of 1.0-10.0 m / h for the liquid in the sedimentation and selection tank, thereby promoting the washing of light flocculent sludge and enriching the granular sludge.

5. The method for cultivating integrated short-cut nitrification anaerobic ammonium oxidation granular sludge according to claim 1, characterized in that: Also includes: The particle retention device is used to retain the particles lost in the effluent and return them to the biological selection tank to reduce the loss of granular sludge.

6. A device for cultivating integrated short-range nitrification anaerobic ammonium oxidation granular sludge, characterized in that: include: The biological selection tank, aeration tank, and sedimentation selection tank are connected in sequence by overflow; The sludge outlet end of the sedimentation selection tank is connected to the biological selection tank through a sludge return pump; The outlet end of the sedimentation and selection tank is provided with a particle interception device and a circulation pump, and part of the outlet water is refluxed to the inlet end of the sedimentation and selection tank through the circulation pump, and the lost particle sludge is intercepted by the particle interception device.

7. The device for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to claim 6, characterized in that: A raw water tank is provided at the water inlet end of the biological selection tank, and aerobic granular sludge is directly added to the biological selection tank and the aeration tank.

8. The device for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to claim 6, characterized in that: An aerator is arranged in the aeration tank, and the aerator is connected to the blower through a gas flow meter.

9. The device for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to claim 6, characterized in that: The precipitation selection tank comprises: A pool body, wherein a mud collecting bucket is provided at the lower end of the pool body, and an overflow weir is provided at the upper end of the pool body; The central tube is arranged in the middle of the tank body, a diffuser with a gradually expanding structure is arranged at the lower end, and an upper end is higher than the overflow weir and connected to the water outlet of the aeration tank.

10. The device for cultivating integrated short-range nitrification and anaerobic ammonium oxidation granular sludge according to claim 6, characterized in that: The particle interception device is connected to the biological selection tank.

Citation Information

Patent Citations

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