Selenium-rich bio-organic fertilizer and preparation method thereof

Abstract

The invention provides a selenium-rich bio-organic fertilizer and a preparation method thereof, and belongs to the technical field of organic fertilizers. The selenium-rich biological organic fertilizer is prepared from the following raw materials: chicken manure, corn straws, aspergillus niger, saccharomycetes, aspergillus oryzae, selenium-rich polyaspartic acid calcium salt, zinc sulfate, boron sulfate, ferrous sulfate, bacillus subtilis, paenibacillus mucilaginosus, chitosan oligosaccharide, iminodisuccinic acid and algal polysaccharides. The selenium-rich polyaspartic acid calcium salt is used as a selenium source, the chicken manure and the corn straw are used as organic raw materials, the organic fertilizer is produced through fermentation by adopting a specific fungicide, and meanwhile, the biostimulant and the seaweed extract are added, so that selenium enrichment of crops is promoted. Meanwhile, the content of water-soluble heavy metal in soil is reduced, the urgent damage of the heavy metal to crop growth is reduced, nutrients such as nitrogen, phosphorus and potassium are fixed, crop growth is promoted, and the crop yield is increased.

Description

[0001] This application is a divisional application. The application number of the original application is: 2023101892880, the application date is 2023.03.02, and the name of the invention is: A poly-tian enzyme selenium-rich biological organic fertilizer and its preparation method. Technical Field

[0002] The invention relates to the technical field of organic fertilizers, and in particular to a selenium-rich biological organic fertilizer and a preparation method thereof. Background Art

[0003] Selenium (Se) is one of the 15 essential nutrients for the human body. A large number of clinical experiments at home and abroad have shown that selenium deficiency in the human body can cause dysfunction of certain important organs and lead to many serious diseases. For example, selenium deficiency can lead to insufficient protein supply and inability to produce enough calories to supply the body's needs, which can lead to malnutrition, growth and development disorders, and then weight loss. Selenium deficiency increases the risk of cardiovascular disease. Selenium deficiency in the human body can cause myocardial cell damage due to the accumulation of lipid peroxides, which cannot protect the myocardium and will increase the incidence and mortality of cardiovascular and cerebrovascular diseases. Studies have shown that people with low selenium or selenium deficiency can not only prevent the occurrence of tumors, liver diseases, etc. by supplementing selenium in moderation, but also improve the body's immune capacity, maintain the normal functions of important organs such as the heart, liver, lungs, and stomach, and prevent the occurrence of cardiovascular and cerebrovascular diseases in the elderly.

[0004] Eating fortified selenium medicine and food preparations is currently the main way to supplement selenium, but these medicine and food preparations have low utilization rates in the human body and have the risk of side effects. Studies have shown that the most easily absorbed selenium by the human body is organic selenium in plants. Plants are the key carriers for the conversion of inorganic selenium into organic selenium in nature. Selenium-rich crops, as important converters of organic selenium, are the best way for humans to ingest selenium.

[0005] Selenium-rich bio-organic fertilizer is a type of biologically active selenium-rich organic fertilizer. The application of selenium-rich bio-organic fertilizer can increase the selenium content in the soil, allowing crops to absorb selenium from the soil to produce selenium-rich agricultural products. Existing selenium-rich fertilizers are mostly directly added with inorganic selenium or organic selenium, which increases the selenium content in the soil, but does not significantly promote the absorption and conversion of selenium by crops. This makes the selenium in the soil excessive after long-term application of selenium-rich bio-organic fertilizer, which easily causes environmental pollution. Therefore, it is very important to provide a new type of selenium-rich bio-organic fertilizer to increase the selenium content of crops and reduce environmental pollution. Summary of the invention

[0006] The object of the present invention is to provide a poly-tian enzyme selenium-enriched biological organic fertilizer and a preparation method thereof. The present invention uses selenium-enriched poly-aspartic acid calcium salt as a selenium source, chicken manure and corn stalks as organic raw materials, adopts a specific bacterial agent to ferment and produce organic fertilizer, and simultaneously adds biostimulants and seaweed extracts to promote the enrichment of selenium in crops. At the same time, the content of water-soluble heavy metals in the soil is reduced, the harm of heavy metals to crop growth is reduced, and nutrients such as nitrogen, phosphorus and potassium are fixed, so as to promote crop growth and increase crop yield.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The invention provides a polytian enzyme selenium-enriched biological organic fertilizer, which is prepared from the following raw materials in parts by weight: 30-40 parts of chicken manure, 30-40 parts of corn stalks, 0.1-0.3 parts of Aspergillus niger, 0.1-0.3 parts of yeast, 0.1-0.3 parts of Aspergillus oryzae, 1-5 parts of selenium-enriched polyaspartic acid calcium salt, 1-5 parts of zinc sulfate, 1-5 parts of boron sulfate, 1-5 parts of ferrous sulfate, 0.1-0.3 parts of Bacillus subtilis, 0.1-0.3 parts of jelly-like Bacillus, 3-8 parts of chitosan oligosaccharide, 0.5-2.0 parts of iminodisuccinic acid, and 4-9 parts of seaweed polysaccharide.

[0009] Preferably, the water content of the chicken manure is 30-55%; the water content of the corn stalks is 45-60%.

[0010] Preferably, the viable count of Aspergillus niger is 4 to 6×10 7 The number of live yeast cells is 1 to 3 × 10 9 The viable count of Aspergillus oryzae is 1 to 3 × 10 9 The viable count of Bacillus subtilis is 2 to 6 × 10 8 The viable bacteria count of the jelly-like Bacillus is 1 to 2×10 7 Pieces / g.

[0011] Preferably, the preparation method of the selenium-rich polyaspartic acid calcium salt is: stirring polyaspartic acid and calcium hydroxide in an aqueous solution for reaction; adding selenious acid to the above solution, filtering, concentrating and drying the filtrate to obtain the selenium-rich polyaspartic acid calcium salt.

[0012] The present invention also provides a method for preparing the above-mentioned polytian enzyme selenium-enriched biological organic fertilizer, comprising the following steps:

[0013] (1) chicken manure, corn stalks, selenium-rich polyaspartic acid calcium salt, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid, and 1 / 4 to 1 / 2 of seaweed polysaccharide are mixed, and Aspergillus niger, yeast, Aspergillus oryzae, Bacillus subtilis, and Paenibacillus jelly are added to the mixture for decomposition and fermentation to obtain primary fertilizer;

[0014] (2) crushing, granulating, drying and screening the primary fertilizer to obtain granular fertilizer;

[0015] (3) spraying a solution of chitosan oligosaccharide on the surface of the granular fertilizer and drying the granular fertilizer to obtain a secondary fertilizer;

[0016] (4) spraying the remaining seaweed polysaccharide solution on the secondary fertilizer and drying it to obtain the polytianzyme selenium-enriched biological organic fertilizer.

[0017] Preferably, the temperature of the composting and fermentation is 25 to 70° C. and the time is 15 to 30 days.

[0018] Preferably, the compost is turned after 5 to 6 days of fermentation, and then every 14 to 16 hours.

[0019] Preferably, in the chitosan oligosaccharide solution, the mass ratio of chitosan oligosaccharide to water is 1:(8-15); in the remaining seaweed polysaccharide solution, the mass ratio of seaweed polysaccharide to water is 1:(8-15).

[0020] The present invention provides a poly-aspartate selenium-enriched bio-organic fertilizer and a preparation method thereof. The present invention uses chicken manure and corn stalks as organic raw materials, adds selenium-enriched poly-aspartate calcium salt and trace elements such as zinc, boron, and iron, adds Bacillus subtilis, Bacillus jelly-like and other microbial agents for composting and fermentation, and utilizes the decomposition effect of the composite agent on the material to increase the fertilizer nutrient content. The present invention uses selenium-enriched poly-aspartate calcium salt as a selenium source, and utilizes its special chemical structure to effectively prevent the volatilization loss of helium fertilizer and the fixation of potassium fertilizer, reduce the content of water-soluble heavy metals in the soil, reduce the persecution of heavy metals on crop growth, promote crop growth, and increase crop yield. At the same time, the present invention supplements biostimulants and seaweed extracts to cooperate with selenium-enriched poly-aspartate calcium salt, improves the absorption capacity of crop nutrients, promotes the enrichment of crops to selenium, and thus increases the selenium content in crops. DETAILED DESCRIPTION

[0021] The invention provides a polytian enzyme selenium-enriched biological organic fertilizer, which is prepared from the following raw materials in parts by weight: 30-40 parts of chicken manure, 30-40 parts of corn stalks, 0.1-0.3 parts of Aspergillus niger, 0.1-0.3 parts of yeast, 0.1-0.3 parts of Aspergillus oryzae, 1-5 parts of selenium-enriched polyaspartic acid calcium salt, 1-5 parts of zinc sulfate, 1-5 parts of boron sulfate, 1-5 parts of ferrous sulfate, 0.1-0.3 parts of Bacillus subtilis, 0.1-0.3 parts of jelly-like Bacillus, 3-8 parts of chitosan oligosaccharide, 0.5-2.0 parts of iminodisuccinic acid, and 4-9 parts of seaweed polysaccharide.

[0022] In the present invention, the polytian enzyme selenium-enriched biological organic fertilizer is preferably made of the following raw materials in parts by weight: 35 parts of chicken manure, 35 parts of corn stalks, 0.2 parts of Aspergillus niger, 0.2 parts of yeast, 0.2 parts of Aspergillus oryzae, 3 parts of selenium-enriched polyaspartic acid calcium salt, 3 parts of zinc sulfate, 3 parts of boron sulfate, 3 parts of ferrous sulfate, 0.2 parts of Bacillus subtilis, 0.2 parts of Bacillus gelatinosa, 5 parts of chitosan oligosaccharides, 1.5 parts of iminodisuccinic acid, and 6 parts of seaweed polysaccharides.

[0023] In the present invention, the water content of the chicken manure is preferably 30-55%, and more preferably 45%.

[0024] In the present invention, the moisture content of the corn stalks is preferably 45-60%, and more preferably 55%.

[0025] In the present invention, the viable count of Aspergillus niger is preferably 4 to 6×10 7 / g, more preferably 5×10 7 pcs / g

[0026] In the present invention, the number of viable yeast is preferably 1 to 3×10 9 / g, more preferably 2×10 9 Pieces / g.

[0027] In the present invention, the viable count of Aspergillus oryzae is preferably 1 to 3×10 9 / g, more preferably 2×10 9 Pieces / g.

[0028] In the present invention, the viable count of the Bacillus subtilis is preferably 2 to 6×10 8 / g, more preferably 4×10 8 Pieces / g.

[0029] In the present invention, the viable count of the jelly-like Bacillus is preferably 1 to 2×10 7 / g, more preferably 1.5×10 7 Pieces / g.

[0030] In the present invention, the preparation method of the selenium-rich polyaspartic acid calcium salt is preferably: stirring polyaspartic acid and calcium hydroxide in an aqueous solution for reaction; adding selenious acid to the above solution, filtering, concentrating and drying the filtrate to obtain the selenium-rich polyaspartic acid calcium salt.

[0031] The present invention also provides a method for preparing the above-mentioned polytian enzyme selenium-enriched biological organic fertilizer, comprising the following steps:

[0032] (1) chicken manure, corn stalks, selenium-rich polyaspartic acid calcium salt, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid, and 1 / 4 to 1 / 2 of seaweed polysaccharide are mixed, and Aspergillus niger, yeast, Aspergillus oryzae, Bacillus subtilis, and Paenibacillus jelly are added to the mixture for decomposition and fermentation to obtain primary fertilizer;

[0033] (2) crushing, granulating, drying and screening the primary fertilizer to obtain granular fertilizer;

[0034] (3) spraying a solution of chitosan oligosaccharide on the surface of the granular fertilizer and drying the granular fertilizer to obtain a secondary fertilizer;

[0035] (4) spraying the remaining seaweed polysaccharide solution on the secondary fertilizer and drying it to obtain the polytianzyme selenium-enriched biological organic fertilizer.

[0036] The invention mixes chicken manure, corn stalks, selenium-rich polyaspartic acid calcium salt, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid and 1 / 4 to 1 / 2 of seaweed polysaccharide, adds aspergillus niger, yeast, aspergillus oryzae, bacillus subtilis and bacillus gelatinous, and performs composting and fermentation to obtain primary fertilizer.

[0037] In the present invention, seaweed polysaccharides are preferably divided into two parts, the first part of seaweed polysaccharides is mixed with chicken manure, corn stalks, selenium-rich polyaspartic acid calcium salt, zinc sulfate, boron sulfate, ferrous sulfate, and iminodisuccinic acid, and Aspergillus niger, yeast, Aspergillus oryzae, Bacillus subtilis, and Bacillus gelatinus are added to decompose and ferment to obtain primary fertilizer.

[0038] In the present invention, the first part of seaweed polysaccharides preferably accounts for 1 / 4 to 1 / 2 of the total weight of the seaweed polysaccharides, and more preferably accounts for 3 / 8 of the total weight of the seaweed polysaccharides.

[0039] In the present invention, the temperature of the composting and fermentation is preferably 25-70°C.

[0040] In the present invention, the fermentation time is preferably 15 to 30 days, more preferably 15 days.

[0041] In the present invention, the compost is preferably turned after 5 to 6 days of fermentation, and then every 14 to 16 hours; more preferably, the compost is turned after 5 days of fermentation, and then every 15 hours.

[0042] The present invention crushes, granulates, dries and sieves the primary fertilizer to obtain granular fertilizer.

[0043] In the present invention, the particle size of the primary fertilizer after pulverization is preferably (0.3 to 2) mm×(0.3 to 2) mm, and more preferably 1 mm×1 mm.

[0044] In the present invention, the drying to a moisture content is preferably 3% to 10%, more preferably 5%.

[0045] In the present invention, the aperture size of the sieve used for screening is preferably 1 to 3 mm, and more preferably 2 mm.

[0046] In the present invention, after the screening, the screened material is preferably taken as granular fertilizer.

[0047] The invention sprays a solution of chitosan oligosaccharide on the surface of the granular fertilizer, and dries the solution to obtain the secondary fertilizer.

[0048] In the present invention, in the chitosan oligosaccharide solution, the mass ratio of chitosan oligosaccharide to water is preferably 1:(8-15), and more preferably 1:12.

[0049] In the present invention, the drying to a moisture content is preferably 3% to 10%, more preferably 5%.

[0050] The invention sprays the second part of the seaweed polysaccharide solution on the secondary fertilizer, and then dries it to obtain the polytianase selenium-enriched biological organic fertilizer.

[0051] In the present invention, the second part of seaweed polysaccharides preferably accounts for 1 / 2 to 3 / 4 of the total weight of the seaweed polysaccharides, and more preferably accounts for 5 / 8 of the total weight of the seaweed polysaccharides.

[0052] In the present invention, in the second portion of the seaweed polysaccharide solution, the mass ratio of seaweed polysaccharide to water is preferably 1:(8-15), and more preferably 1:12.

[0053] In the present invention, the drying to a moisture content is preferably 3% to 10%, more preferably 5%.

[0054] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0055] Example 1

[0056] The invention provides a polytian enzyme selenium-enriched biological organic fertilizer, which comprises 35kg of chicken manure with a water content of 45%, 35kg of corn stalks with a water content of 55%, 0.2kg of Aspergillus niger, 0.2kg of yeast, 0.2kg of Aspergillus oryzae, 3kg of selenium-enriched polyaspartic acid calcium salt, 3kg of zinc sulfate, 3kg of boron sulfate, 3kg of ferrous sulfate, 0.2kg of Bacillus subtilis, 0.2kg of Paenibacillus gelatinus, 5kg of chitosan oligosaccharide, 1.5kg of iminodisuccinic acid and 6kg of seaweed polysaccharide.

[0057] Among them, the selenium-rich polyaspartic acid calcium salt is prepared according to the production method disclosed in Chinese patent CN103275318 B, and the specific process is as follows: 5000g of polyaspartic acid and 232g of calcium hydroxide are stirred and reacted in an aqueous solution at 50°C; selenious acid is added to the above solution until the pH value of the solution is 3.5, filtered, and the filtrate is concentrated and dried to obtain the selenium-rich polyaspartic acid calcium salt.

[0058] The specific preparation process of Jutian enzyme selenium-rich biological organic fertilizer is as follows:

[0059] The seaweed polysaccharide was divided into two parts according to the ratio of 3:5. Chicken manure, corn stalks, calcium salt of selenium-rich polyaspartate, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid, and the seaweed polysaccharide of the first part (3 / 8 of the total mass) were mixed evenly. Aspergillus niger (viable count 5×10 7 / g), yeast (viable count 2×10 9 / g), Aspergillus oryzae (viable count 2×10 9 Bacillus subtilis (viable count 4×10 8 / g), jelly-like Bacillus (viable count 1.5×10 7 / g), stir evenly and cover the material for composting and fermentation. Measure the temperature at the center of the pile every day, turn the pile on the 5th day of fermentation, and then turn it every 15 hours to control the temperature in the material pile within the range of 25-55℃. When the temperature in the pile drops below 30℃, the odor of the material decreases, the texture becomes loose, and the fermentation is completed to obtain primary fertilizer.

[0060] The primary fertilizer is crushed into 1mm×1mm, placed in a granulator for granulation, taken out and dried to a moisture content of 5%, passed through a 2mm sieve, and the sieve is taken to obtain granular fertilizer. According to the mass ratio of 1:12, chitosan oligosaccharide solution is prepared with water; according to the mass ratio of 1:12, the second part (5 / 8 of the total mass) of seaweed polysaccharide is dissolved with water to prepare a solution. Chitosan oligosaccharide is evenly sprayed on the surface of the granular fertilizer, and then the fertilizer is dried to a moisture content of 5% to obtain a secondary fertilizer; the seaweed polysaccharide solution is evenly sprayed on the surface of the secondary fertilizer, and the fertilizer is dried again to a moisture content of 5% to obtain a polytian enzyme selenium-enriched biological organic fertilizer.

[0061] Example 2

[0062] The invention provides a polytian enzyme selenium-enriched biological organic fertilizer, which comprises 30kg of chicken manure with a water content of 30%, 30kg of corn stalks with a water content of 45%, 0.1kg of Aspergillus niger, 0.1kg of yeast, 0.1kg of Aspergillus oryzae, 1kg of selenium-enriched polyaspartic acid calcium salt, 1kg of zinc sulfate, 1kg of boron sulfate, 1kg of ferrous sulfate, 0.1kg of Bacillus subtilis, 0.1kg of Paenibacillus gelatinus, 3kg of chitosan oligosaccharide, 0.5kg of iminodisuccinic acid and 4kg of seaweed polysaccharide.

[0063] Among them, the selenium-rich polyaspartic acid calcium salt is prepared according to the production method disclosed in Chinese patent CN103275318 B, and the specific process is as follows: 5000g of polyaspartic acid and 232g of calcium hydroxide are stirred and reacted in an aqueous solution at 50°C; selenious acid is added to the above solution until the pH value of the solution is 3.5, filtered, and the filtrate is concentrated and dried to obtain the selenium-rich polyaspartic acid calcium salt.

[0064] The specific preparation process of Jutian enzyme selenium-rich biological organic fertilizer is as follows:

[0065] The seaweed polysaccharide was divided into two parts according to the ratio of 1:3. Chicken manure, corn stalks, calcium salt of selenium-rich polyaspartate, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid, and the seaweed polysaccharide of the first part (1 / 4 of the total mass) were mixed evenly. Aspergillus niger (viable count 4×10 7 / g), yeast (viable count 1×10 9 / g), Aspergillus oryzae (viable count 1×10 9 / g), Bacillus subtilis (viable count 2×10 8 / g), jelly-like Bacillus (viable count 1×10 7 / g), stir evenly and cover the material for composting and fermentation. Measure the temperature at the center of the pile every day. Turn the pile on the 6th day of fermentation, and then turn it every 15 hours to control the temperature in the material pile within the range of 25-55℃. When the temperature in the pile drops below 30℃, the odor of the material decreases, the texture becomes loose, and the fermentation is completed, and primary fertilizer is obtained.

[0066] The primary fertilizer is crushed into 1mm×1mm, placed in a granulator for granulation, taken out and dried to a moisture content of 5%, passed through a 2mm sieve, and the sieve is taken to obtain granular fertilizer. According to a mass ratio of 1:8, chitosan oligosaccharide solution is prepared with water; according to a mass ratio of 1:8, the second part of seaweed polysaccharide (3 / 4 of the total mass) is dissolved with water to prepare a solution. Chitosan oligosaccharide is evenly sprayed on the surface of the granular fertilizer, and then the fertilizer is dried to a moisture content of 5% to obtain a secondary fertilizer; the seaweed polysaccharide solution is evenly sprayed on the surface of the secondary fertilizer, and the fertilizer is dried again to a moisture content of 5% to obtain a polytian enzyme selenium-enriched biological organic fertilizer.

[0067] Example 3

[0068] The invention provides a polytian enzyme selenium-enriched biological organic fertilizer, which comprises 40kg of chicken manure with a water content of 55%, 40kg of corn stalks with a water content of 60%, 0.3kg of Aspergillus niger, 0.3kg of yeast, 0.3kg of Aspergillus oryzae, 5kg of selenium-enriched polyaspartic acid calcium salt, 5kg of zinc sulfate, 5kg of boron sulfate, 5kg of ferrous sulfate, 0.3kg of Bacillus subtilis, 0.3kg of Paenibacillus gelatinus, 8kg of chitosan oligosaccharide, 2.0kg of iminodisuccinic acid and 9kg of seaweed polysaccharide.

[0069] Among them, the selenium-rich polyaspartic acid calcium salt is prepared according to the production method disclosed in Chinese patent CN103275318 B, and the specific process is as follows: 5000g of polyaspartic acid and 232g of calcium hydroxide are stirred and reacted in an aqueous solution at 50°C; selenious acid is added to the above solution until the pH value of the solution is 3.5, filtered, and the filtrate is concentrated and dried to obtain the selenium-rich polyaspartic acid calcium salt.

[0070] The specific preparation process of Jutian enzyme selenium-rich biological organic fertilizer is as follows:

[0071] The seaweed polysaccharide was divided into two parts according to the ratio of 1:1. Chicken manure, corn stalks, calcium salt of selenium-rich polyaspartate, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid, and the seaweed polysaccharide of the first part (1 / 2 of the total mass) were mixed evenly. Aspergillus niger (viable count 5×10 7 / g), yeast (viable count 2×10 9 / g), Aspergillus oryzae (viable count 2×10 9 Bacillus subtilis (viable count 4×10 8 / g), jelly-like Bacillus (viable count 1.5×10 7 / g), stir evenly and cover the material for composting and fermentation. Measure the temperature at the center of the pile every day, turn the pile on the 5th day of fermentation, and then turn it every 15 hours to control the temperature in the material pile within the range of 25-55℃. When the temperature in the pile drops below 30℃, the odor of the material decreases, the texture becomes loose, and the fermentation is completed to obtain primary fertilizer.

[0072] The primary fertilizer is crushed into 1mm×1mm, placed in a granulator for granulation, taken out and dried to a moisture content of 5%, passed through a 2mm sieve, and the sieve is taken to obtain granular fertilizer. According to the mass ratio of 1:12, chitosan oligosaccharide solution is prepared with water; according to the mass ratio of 1:12, the second part of seaweed polysaccharide (1 / 2 of the total mass) is dissolved with water to prepare a solution. Chitosan oligosaccharide is evenly sprayed on the surface of the granular fertilizer, and then the fertilizer is dried to a moisture content of 5% to obtain a secondary fertilizer; the seaweed polysaccharide solution is evenly sprayed on the surface of the secondary fertilizer, and the fertilizer is dried again to a moisture content of 5% to obtain a polytian enzyme selenium-enriched biological organic fertilizer.

[0073] Comparative Example 1

[0074] This comparative example also explored the effect of the addition amount of Aspergillus niger, yeast and Aspergillus oryzae on the composting and fermentation process of organic fertilizer during the preparation of polytian enzyme selenium-enriched biological organic fertilizer.

[0075] The addition amount of Aspergillus niger was set to 0.05 kg, the addition amount of yeast was set to 0.05 kg, and the addition amount of Aspergillus oryzae was set to 0.05 kg for comparison group 1; the addition amount of Aspergillus niger was adjusted to 0.5 kg, and the addition amount of yeast was adjusted to 0.5 kg for comparison group 2. Other treatments were the same as in Example 1.

[0076] Comparative Example 2

[0077] This comparative example also explored the influence of the types of fermentation agents on the composting and fermentation process of organic fertilizer during the preparation of polytian enzyme selenium-enriched biological organic fertilizer.

[0078] A comparative group 3 was set up with Trichoderma viride, yeast, Aspergillus oryzae, Bacillus subtilis, and Paenibacillus jelly as fermentation agents; and a comparative group 4 was set up with Aspergillus niger, yeast, Aspergillus oryzae, Bacillus licheniformis, and Paenibacillus jelly as fermentation agents. Other treatments were the same as in Example 1.

[0079] Comparative Example 3

[0080] This comparative example also explored the influence of the types of selenium source materials on the composting and fermentation process of organic fertilizer during the preparation of polytian enzyme selenium-enriched biological organic fertilizer.

[0081] The selenium-rich polyaspartic acid calcium salt was replaced with calcium selenite, which was set as control group 5. The other treatments were the same as in Example 1.

[0082] Test Example 4

[0083] In this comparative example, chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide were omitted, and the comparative group was set as comparative group 6. According to the preparation process of Example 1, the steps of adding chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide were omitted accordingly.

[0084] Comparative Example 5

[0085] In this comparative example, chitosan oligosaccharide is replaced by chitosan, iminodisuccinic acid is replaced by humic acid, and seaweed polysaccharide is replaced by lentinan, which is set as comparative group 7. Other treatments are the same as in Example 1.

[0086] Comparative Example 6

[0087] This comparative example adopts the raw material composition and dosage of Example 1, adjusts the preparation process, and sets it as comparative group 8. The specific preparation process is adjusted as follows:

[0088] Chicken manure, corn stalks, calcium salt of selenium-rich polyaspartate, zinc sulfate, boron sulfate, ferrous sulfate, and iminodisuccinic acid were mixed evenly, and Aspergillus niger (viable count 5×10 7 / g), yeast (viable count 2×10 9 / g), Aspergillus oryzae (viable count 2×10 9 Bacillus subtilis (viable count 4×10 8 / g), jelly-like Bacillus (viable count 1.5×10 7 / g), stir evenly and cover the material for composting and fermentation. Measure the temperature at the center of the pile every day, turn the pile on the 5th day of fermentation, and then turn it every 15 hours. When the temperature in the pile drops below 30℃, the odor of the material decreases, the texture becomes loose, and the fermentation is completed, primary fertilizer is obtained.

[0089] The primary fertilizer is crushed into 1mm×1mm, placed in a granulator for granulation, taken out and dried to a moisture content of 5%, passed through a 2mm sieve, and the sieve is taken to obtain granular fertilizer. Chitosan oligosaccharide solution is prepared with water at a mass ratio of 1:12; seaweed polysaccharide is dissolved in water at a mass ratio of 1:12 to prepare a solution. Chitosan oligosaccharide is evenly sprayed on the surface of granular fertilizer, and then the fertilizer is dried to a moisture content of 5% to obtain secondary fertilizer; seaweed polysaccharide solution is evenly sprayed on the surface of secondary fertilizer, and the fertilizer is dried again to a moisture content of 5% to obtain polytian enzyme selenium-enriched biological organic fertilizer.

[0090] Test Example 1

[0091] The performance of the selenium-rich biological organic fertilizers in each group of Examples 1 to 3 and Comparative Examples 1 to 6 was evaluated by taking the temperature change, the decomposition time, and the nutrient content of the organic fertilizer as indicators during the decomposition and fermentation of the organic fertilizer. The test results are shown in Tables 1 to 2.

[0092] Table 1 Temperature changes during the fermentation of each group of selenium-rich biological organic fertilizer

[0093]

[0094]

[0095] As can be seen from Table 1, the selenium-rich biological organic fertilizers of Examples 1 to 3 heat up quickly during the fermentation process, and can quickly kill harmful components in the material. The high temperature above 60°C lasts for about three days, and the subsequent temperature drops to about 50°C, which can meet the activity temperature of the fermentation agent, accelerate the material decomposition process, and shorten the fermentation time. After the comparison group 1 reduces the addition amount of the fermentation agent, the temperature rises slowly, and it takes 8 days to heat up to above 60°C, which significantly prolongs the decomposition time. After the comparison group 2 increases the addition amount of the fermentation agent, the temperature rises quickly, and the temperature can be raised to 62°C on the second day of fermentation, but the temperature drops quickly, which easily causes the problem of incomplete decomposition. The comparison group 3 replaces Aspergillus niger with Trichoderma viride, and the comparison group 4 replaces Bacillus subtilis with Bacillus licheniformis. After these two groups are replaced, the time used for decomposition fermentation is significantly extended. After replacing Bacillus subtilis, it takes 30 days to complete the decomposition. The comparison group 5 replaces the selenium-rich polyaspartic acid calcium salt, which has no obvious effect on the decomposition fermentation process compared with Example 1. Comparative group 6 omitted chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide, and compared with Examples 2 and 3, there was no significant effect on the fermentation process. Comparative group 7 replaced chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide, and compared with Examples 2 and 3, there was no significant effect on the fermentation process. Comparative group 8 adjusted the timing of adding seaweed polysaccharide, slowed down the heating rate, and prolonged the fermentation time. It shows that adding seaweed polysaccharide during the fermentation process can promote the fermentation process.

[0096] Table 2 Nutrient content of each group of selenium-enriched biological organic fertilizer

[0097]

[0098]

[0099] As can be seen from Table 2, the organic matter and nitrogen contents in the selenium-rich biological organic fertilizers of Examples 1 to 3 are higher than those in Comparative Groups 1 to 8. Among them, after the addition amount of fermentation agent was reduced in Comparative Group 1, the organic matter content in the organic fertilizer decreased, while after the addition amount of fermentation agent was increased in Comparative Group 2, the organic matter and nitrogen contents in the organic fertilizer did not increase significantly. This shows that the amount of fermentation agent has a certain effect on the quality of organic fertilizer. Comparative Group 3 replaced Aspergillus niger with Trichoderma viride, and Comparative Group 4 replaced Bacillus subtilis with Bacillus licheniformis. The organic matter and nitrogen contents in the two groups of organic fertilizers decreased, indicating that the type of fermentation agent has a certain effect on the quality of organic fertilizer. Comparative Group 5 replaced selenium-rich polyaspartic acid calcium salt. Compared with the embodiment, the organic matter and nitrogen contents in the organic fertilizer did not fluctuate significantly. Comparative Group 6 omitted chitosan oligosaccharides, iminodisuccinic acid and seaweed polysaccharides, and the organic matter and nitrogen contents in the organic fertilizer decreased, but the decrease was small. In comparison group 7, chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide were replaced, and the organic matter and nitrogen content in the organic fertilizer decreased, but the decrease was small. In comparison group 8, the timing of adding seaweed polysaccharide was adjusted, and the organic matter and nitrogen content in the organic fertilizer decreased.

[0100] Test Example 2

[0101] 12 test fields with the same area and fertility were selected to plant wheat. The selenium-rich bio-organic fertilizers prepared in Examples 1 to 3 and Comparative Groups 5 to 7 were used as base fertilizers at a rate of 20 kg / mu. Wheat was sown after application, and the sowing rate of wheat was 6 kg / mu. After the wheat seedlings emerged, weeds were removed in time and watered, preferably thoroughly on the same day; urea was applied at the jointing stage, 15 kg per mu; ammonium bicarbonate was applied at the booting stage, 5 kg per mu. After the wheat matured, the wheat yield and selenium content of each group were counted. The results are shown in Table 3.

[0102] Table 3 Wheat yield and yield of each group

[0103] Group Thousand-grain weight (g) Yield (kg / mu) Selenium content (μg / kg) Example 1 43.6 436.8 438 Example 2 42.8 416.9 439 Example 3 42.7 432.1 435 Comparison group 5 33.8 355.5 321 Comparison group 6 36.4 371.3 368 Comparison group 7 37.1 385.6 351

[0104] As can be seen from Table 4, the selenium-enriched biological organic fertilizers of Examples 1 to 3 can significantly increase the thousand-grain weight and per-acre yield of wheat, and can significantly increase the selenium content in wheat to obtain selenium-enriched wheat. Comparing Examples 1 to 3 with Comparative Group 5, it can be seen that the present invention uses selenium-enriched polyaspartic acid calcium salt as a selenium source, which can promote the absorption of selenium by wheat; comparing Examples 1 to 3, Comparative Group 5 and Comparative Group 6, it can be seen that the chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide added by the present invention can further promote the absorption of selenium in selenium-enriched polyaspartic acid calcium salt by wheat; comparing Examples 1 to 3 with Comparative Group 7, it can be seen that after replacing chitosan oligosaccharide, iminodisuccinic acid and seaweed polysaccharide, the other ingredients cannot effectively promote the absorption of selenium by wheat.

[0105] Test Example 3

[0106] The soil of the test field before wheat sowing and after wheat harvest in Test Example 2 was collected, and the content of water-soluble heavy metals (Cd, As) in the soil was measured to evaluate the passivation effect of each group of selenium-enriched biological organic fertilizer on heavy metals in the soil. The test results are shown in Table 4.

[0107] Table 4 Changes in water-soluble heavy metal content in each group of selenium-enriched biological organic fertilizer before and after fermentation

[0108]

[0109]

[0110] As can be seen from Table 4, before and after the application of the selenium-rich biological organic fertilizers of Examples 1 to 3, the content of water-soluble heavy metals in the soil can be effectively reduced, and the heavy metals in the soil can be passivated. Comparative groups 6 and 7 also have a significant effect on reducing water-soluble heavy metals, but comparative group 5 has no significant effect on the content of water-soluble heavy metals in the soil. It shows that selenium-rich polyaspartic acid calcium salt has the effect of passivating soil heavy metals, which can indirectly reduce the persecution of heavy metals on crop growth, promote crop growth, and increase crop yield.

[0111] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A selenium-rich biological organic fertilizer, characterized in that: The invention is prepared from the following raw materials in parts by weight: 30 parts of chicken manure, 30 parts of corn stalks, 0.1 parts of Aspergillus niger, 0.1 parts of yeast, 0.1 parts of Aspergillus oryzae, 1 part of selenium-rich polyaspartic acid calcium salt, 1 part of zinc sulfate, 1 part of boron sulfate, 1 part of ferrous sulfate, 0.1 parts of Bacillus subtilis, 0.1 parts of Paenibacillus gelatinus, 3 parts of chitosan oligosaccharide, 0.5 parts of iminodisuccinic acid, and 4 parts of seaweed polysaccharide; The method for preparing the organic fertilizer comprises the following steps: (1) chicken manure, corn stalks, selenium-rich polyaspartic acid calcium salt, zinc sulfate, boron sulfate, ferrous sulfate, iminodisuccinic acid, and 1 / 4 to 1 / 2 of seaweed polysaccharide are mixed, and Aspergillus niger, yeast, Aspergillus oryzae, Bacillus subtilis, and Paenibacillus jelly are added to the mixture for decomposition and fermentation to obtain primary fertilizer; (2) crushing, granulating, drying and screening the primary fertilizer to obtain granular fertilizer; (3) spraying a solution of chitosan oligosaccharide on the surface of the granular fertilizer and drying the granular fertilizer to obtain a secondary fertilizer; (4) Spraying the remaining seaweed polysaccharide solution on the secondary fertilizer and drying it to obtain the selenium-rich polyaspartic acid calcium salt biological organic fertilizer.

2. The bio-organic fertilizer according to claim 1, characterized in that The water content of the chicken manure is 30-55%; the water content of the corn stalks is 45-60%.

3. The bio-organic fertilizer according to claim 2, characterized in that The viable count of Aspergillus niger is 4 to 6×10 7 The number of live yeast cells is 1 to 3 × 10 9 The viable count of Aspergillus oryzae is 1 to 3 × 10 9 The viable count of Bacillus subtilis is 2 to 6 × 10 8 The viable bacteria count of the jelly-like Bacillus is 1 to 2×10 7 Pieces / g.

4. The bio-organic fertilizer according to claim 3, characterized in that The preparation method of the selenium-rich polyaspartic acid calcium salt is as follows: polyaspartic acid and calcium hydroxide are stirred and reacted in an aqueous solution; selenious acid is added to the solution, filtered, and the filtrate is concentrated and dried to obtain the selenium-rich polyaspartic acid calcium salt.

5. The bio-organic fertilizer according to claim 4, characterized in that The temperature of the fermentation in step (1) is 25 to 70° C. and the time is 15 to 30 days.

6. The bio-organic fertilizer according to claim 5, characterized in that The compost is turned after 5 to 6 days of fermentation, and then every 14 to 16 hours.

7. The bio-organic fertilizer according to claim 6, characterized in that In the chitosan oligosaccharide solution of step (3), the mass ratio of chitosan oligosaccharide to water is 1:(8-15); in the remaining seaweed polysaccharide solution, the mass ratio of seaweed polysaccharide to water is 1:(8-15).

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