A curve image compensation method for thin layer chromatography scanner

Abstract

The present invention relates to the field of chromatography testing technology, and specifically to a curve image compensation method for a thin layer chromatography scanner, comprising: obtaining the degree of abnormal separation effect of any peak on the initial chromatography curve of any thin layer plate, obtaining the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated, regulating the mobile phase flow rate of all thin layer plates according to the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated, and using the chromatography curve of any thin layer plate after the mobile phase flow rate is regulated as the compensation curve of the initial chromatography curve of the thin layer plate. The present invention improves the situation where the separation of the sample to be tested is not good due to the uneven surface of the corresponding thin layer plate, reduces the situation of abnormal peak shape in the corresponding curve, and realizes effective compensation of the chromatography curve.

Description

Technical Field

[0001] The invention relates to the technical field of chromatography testing, and in particular to a curve image compensation method for a thin layer chromatography scanner. Background Art

[0002] Thin layer chromatography is an effective means of tracking reactions. It can quickly separate mixed samples and perform qualitative analysis on small amounts of substances. It uses the differences in adsorption capacity of each component to be tested on the same adsorbent to separate the components from each other. Due to its low cost and rapid detection, thin layer chromatography is currently widely used in fields such as drug analysis.

[0003] The commonly used thin layer chromatography scanner at present is a radioactive thin layer chromatography scanner. The accuracy of the chromatographic curve obtained by it is affected by the uniformity of the thin layer plate. There may be uneven coating of the stationary phase or uneven drying of the support plate surface, which makes the uniformity of the thin layer plate poor, thereby causing the thin layer chromatography curve to appear double peaks. When using a thin layer chromatograph for detection, the existing technology usually uses the method of changing the material of the thin layer plate to improve the above problems, but it is unable to perform dynamic regulation according to different situations. Summary of the invention

[0004] The invention provides a curve image compensation method for a thin layer chromatography scanner to solve the existing problems.

[0005] A curve image compensation method for a thin layer chromatography scanner of the present invention adopts the following technical scheme:

[0006] An embodiment of the present invention provides a curve image compensation method for a thin layer chromatography scanner, the method comprising the following steps:

[0007] Obtaining initial chromatographic curves of several thin layer plates, wherein the initial chromatographic curves include a response signal corresponding to each acquisition time within a preset time, and obtaining several peaks of each initial chromatographic curve;

[0008] According to the difference in the profiles of the peaks between the initial chromatographic curves of the thin layer plates, the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate is obtained; according to the difference in the number of peaks on the initial chromatographic curves of any thin layer plate and other thin layer plates, and the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curves of any thin layer plate and other thin layer plates, the degree to which the flow rate of the mobile phase needs to be adjusted for the thin layer plate is obtained;

[0009] The mobile phase flow rate is adjusted for all thin layer plates according to the degree to which the mobile phase flow rate needs to be adjusted for the thin layer plates, and the chromatographic curve of any thin layer plate after the mobile phase flow rate adjustment is used as the compensation curve of the initial chromatographic curve of the thin layer plate.

[0010] Furthermore, the specific method of obtaining the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate according to the difference in peak profiles between the initial chromatographic curves of the thin layer plate includes:

[0011] Obtaining the abnormality degree of any peak in the initial chromatographic curve of any thin layer plate;

[0012] The method for obtaining the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate is as follows:

[0013]

[0014] in, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks; Indicates The initial chromatographic curve of the thin layer plate The area of ​​the peak, Indicates The initial chromatographic curve of the thin layer plate The abnormality of each peak, Indicates that except The initial chromatographic curves of all thin layer plates The mean of the areas of the peaks; Indicates The initial chromatographic curve of the thin layer plate The peak width of the peak, Indicates that except The initial chromatographic curves of all thin layer plates The mean of the peak widths of the peaks; represents the absolute value function.

[0015] Furthermore, the specific method for obtaining the abnormality degree of any peak in the initial chromatographic curve of any thin layer plate is:

[0016] For any peak in the initial chromatographic curve of any thin layer plate, the difference between the maximum response signal of the peak and the corresponding peak in the initial chromatographic curves of all other thin layer plates is calculated and the absolute value is taken, and the absolute values ​​of all the differences are summed up and recorded as the abnormality degree of any peak in the initial chromatographic curve of the thin layer plate;

[0017] The corresponding peaks are peaks with the same serial numbers on the initial chromatographic curves of different thin layer plates.

[0018] Furthermore, the degree to which the flow rate of the mobile phase needs to be regulated on the thin layer plate is obtained according to the difference in the number of peaks on the initial chromatographic curve between any thin layer plate and the remaining thin layer plates, and the difference in the degree of abnormality of the separation effect of all peaks on the initial chromatographic curve between any thin layer plate and the remaining thin layer plates, including the specific method of:

[0019] According to the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curve of any thin layer plate and the remaining thin layer plates, the fluctuation degree of the abnormal degree of separation effect of the peaks on the initial chromatographic curve of the thin layer plate is obtained;

[0020] The method to obtain the degree of control of mobile phase flow rate for any thin layer plate is:

[0021]

[0022] in, Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates that except The average number of peaks on the initial chromatographic curves of all thin layer plates; Indicates The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curve of the thin layer plate, Indicates that except The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curves of all thin layer plates of a thin layer plate; Indicates The degree of fluctuation of the abnormality of the peak separation effect on the initial chromatographic curve of each thin layer plate; Represents the normalization function.

[0023] Furthermore, the method of obtaining the fluctuation degree of the abnormal degree of separation effect of the peaks on the initial chromatographic curve of the thin layer plate according to the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curve of any thin layer plate and the remaining thin layer plates includes the following specific methods:

[0024] The degree of abnormality in separation effect of each peak on the initial chromatographic curve of any thin layer plate is subtracted from the mean value of the degree of abnormality in separation effect of all peaks on the initial chromatographic curve of the thin layer plate and the absolute value is taken. The absolute value of each difference is summed and recorded as the degree of fluctuation of the degree of abnormality in separation effect of the peaks on the initial chromatographic curve of the thin layer plate.

[0025] Furthermore, the mobile phase flow rate is regulated for all thin layer plates according to the degree to which the mobile phase flow rate needs to be regulated for the thin layer plates, and the specific method includes:

[0026] The degree to which the mobile phase flow rate needs to be regulated for any thin layer plate is added to the preset first parameter, and the obtained value is multiplied by the mobile phase flow rate of the thin layer plate to obtain the regulated mobile phase flow rate of the thin layer plate.

[0027] Further, after obtaining the compensation curve of the initial chromatographic curve of the thin layer plate, the abnormal thin layer plate is screened according to the initial chromatographic curve of the thin layer plate and the compensation curve. The specific method includes:

[0028] The difference in the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the mobile phase flow rate is adjusted, and the difference in the abnormal degree of separation effect of all corresponding peaks on the initial chromatographic curve and compensation curve of the thin layer plate before and after the mobile phase flow rate are combined to obtain the effective degree of regulation of any thin layer plate; the difference in the effective degree of regulation between any thin layer plate and all thin layer plates, and the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the regulation are combined to determine the degree to which the thin layer plate needs maintenance, and abnormal thin layer plates are screened according to the degree to which the thin layer plates need maintenance.

[0029] Furthermore, the difference in the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated before and after the mobile phase flow rate is regulated, and the difference in the degree of abnormal separation effect of all corresponding peaks on the initial chromatographic curve and the compensation curve of the thin layer plate before and after the mobile phase flow rate is fused to obtain the regulation effectiveness of any thin layer plate, including the specific method of:

[0030] Obtain the degree of change in the degree to which the mobile phase flow rate needs to be adjusted for any thin layer plate before and after adjustment;

[0031] The method for obtaining the control effectiveness of any thin layer plate is:

[0032]

[0033] in, Indicates The effectiveness of the regulation of each thin layer plate; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate; Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks, Indicates The compensation curve of the initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks; Indicates The average value of the abnormal degree of separation effect of all peaks on the compensation curve of the initial chromatographic curve of the thin layer plate; represents the absolute value function.

[0034] Furthermore, the specific method for obtaining the degree of change in the degree to which the mobile phase flow rate needs to be regulated for any thin layer plate before and after regulation is:

[0035] Obtain the abnormal degree of separation effect of any peak on the compensation curve of the initial chromatographic curve of any thin layer plate, and the degree to which the mobile phase flow rate needs to be adjusted after the thin layer plate is adjusted;

[0036] The difference between the degree to which the mobile phase flow rate needs to be regulated for any thin layer plate and the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate after the regulation is calculated and the absolute value is taken. The absolute value of the difference is compared with the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate after the regulation, and is recorded as the degree of change in the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate.

[0037] Furthermore, the difference in the effective degree of regulation between any thin layer plate and all thin layer plates, and the degree to which the thin layer plate needs to regulate the flow rate of the mobile phase before and after regulation are integrated to determine the degree to which the thin layer plate needs maintenance, and the abnormal thin layer plate is screened according to the degree to which the thin layer plate needs maintenance, including the specific method of:

[0038] The degree of maintenance required for any thin layer plate can be obtained by:

[0039]

[0040] in, Indicates The degree to which the laminate requires maintenance; Indicates The effectiveness of the regulation of the thin layer plate, It represents the mean value of the regulation effectiveness of all thin layer plates; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates that after regulation The degree to which the mobile phase flow rate needs to be controlled for each thin layer plate; represents the absolute value function, represents the normalization function;

[0041] A thin layer plate whose maintenance requirement is greater than a preset second parameter is recorded as an abnormal thin layer plate.

[0042] The beneficial effect of the technical solution of the present invention is as follows: this embodiment obtains peaks that may be abnormal in the separation effect of the sample to be tested by comparing the difference in response signals of multiple thin layer plates processing the same sample to be tested under the same conditions, and adjusts the flow rate of the mobile phase according to the degree of abnormality, thereby improving the situation where the sample to be tested is not well separated due to the uneven surface of the corresponding thin layer plate, reducing the situation where the peak shape in the corresponding curve is abnormal, and using the chromatographic curve after adjustment as a compensation curve to achieve effective compensation for the chromatographic curve. At the same time, this embodiment also screens out abnormal thin layer plates by analyzing the effectiveness of the adjustment, which is conducive to reducing the analysis and use of abnormal thin layer plates, and improving the efficiency of the experiment and the accuracy of the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0044] Figure 1 The present invention is a flow chart of the steps of a curve image compensation method for a thin layer chromatography scanner. DETAILED DESCRIPTION

[0045] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following is a detailed description of a curve image compensation method for a thin layer chromatography scanner proposed by the present invention, its specific implementation, structure, features and effects, in conjunction with the accompanying drawings and preferred embodiments. In the following description, different "one embodiment" or "another embodiment" does not necessarily refer to the same embodiment. In addition, specific features, structures or characteristics in one or more embodiments may be combined in any suitable form.

[0046] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0047] The following is a detailed description of a specific scheme of a curve image compensation method for a thin layer chromatography scanner provided by the present invention in conjunction with the accompanying drawings.

[0048] See also Figure 1 , which shows a flow chart of a curve image compensation method for a thin layer chromatography scanner provided by an embodiment of the present invention, the method comprising the following steps:

[0049] Step S001: obtaining initial chromatographic curves of several thin layer plates, and obtaining several peaks on each initial chromatographic curve.

[0050] It should be noted that this embodiment takes the thin layer plate chromatography curve image obtained by a radioactive thin layer chromatograph as an example.

[0051] It should be noted that in the actual working process, the thickness of multiple different thin layer plates of the same material may be different, the coating of the stationary phase may be uneven, etc., which may affect the performance of different thin layer plates in separating the samples to be tested. As a result, when processing the same sample to be tested, there are large differences in the response signals obtained from multiple thin layer plates of the same material at the same time. Therefore, when a radioactive thin layer chromatography scanner is used to obtain a group of samples to be tested that process the same sample to be tested, the changes in the response signals of multiple thin layer plates of the same size and material are obtained.

[0052] Specifically, in order to implement the curve image compensation method for a thin layer chromatography scanner proposed in this embodiment, it is first necessary to collect the initial chromatographic curve of the thin layer plate. The specific process is as follows:

[0053] The sample groups to be tested are coated on thin layer plates of the same size and material respectively; the samples to be tested on the thin layer plates are developed at the same mobile phase flow rate; and the initial chromatographic curve corresponding to each thin layer plate is obtained by a radioactive thin layer chromatograph.

[0054] The initial chromatographic curve includes a response signal corresponding to each acquisition time within a preset time, and the portion between any two consecutive troughs on the initial chromatographic curve is recorded as a peak.

[0055] It should be noted that the mobile phase refers to the solvent that transports the sample to be tested from the injection port to the separation column, and the mobile phase flow rate refers to the flow rate of the mobile phase in the liquid chromatography instrument. The mobile phase flow rate directly affects the separation effect and separation time of the sample to be tested on the thin layer plate. If the flow rate is too fast, some samples cannot be separated. If the flow rate is too slow, the separation time will be longer.

[0056] It should be noted that the separation effect is manifested by the initial chromatographic curve corresponding to the thin layer plate of the sample to be tested. Therefore, it is necessary to obtain the sample to be tested with abnormal development by analyzing the abnormal performance of the initial chromatographic curve, and to adjust the flow rate of the mobile phase of the sample to be tested with abnormal development, so as to obtain the compensation curve of the initial chromatographic curve and complete the curve image compensation.

[0057] It should be noted that the preset time is set to 15 minutes based on experience and can be adjusted according to actual conditions, and this embodiment does not specifically limit it.

[0058] It should be noted that the unit corresponding to the acquisition time is min, and the unit corresponding to the response signal is mAu.

[0059] So far, the initial chromatographic curves of several thin layer plates have been obtained by the above method.

[0060] Step S002: according to the difference in the profiles of the peaks between the initial chromatographic curves of the thin layer plates, obtain the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate; according to the difference in the number of peaks on the initial chromatographic curves between any thin layer plate and the remaining thin layer plates, and the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curves between any thin layer plate and the remaining thin layer plates, obtain the degree to which the flow rate of the mobile phase needs to be adjusted for the thin layer plate.

[0061] It should be noted that the present embodiment aims to regulate the thin layer plate with abnormal separation effect, and therefore it is necessary to first obtain the thin layer plate with abnormal separation effect, and the abnormal separation effect is mainly manifested in the abnormal initial chromatographic curve of the thin layer plate, and the abnormal initial chromatographic curve is manifested in the number, area, and peak width characteristics of the peaks compared with other thin layer plates, and therefore the degree of abnormal separation effect of any peak can be obtained through the characteristic abnormalities of the peaks of the thin layer plate, and then the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate can be obtained according to the difference in the degree of abnormal separation effect of the corresponding peaks on the initial chromatographic curves between the thin layer plates, and finally the mobile phase flow rate is regulated.

[0062] Specifically, in step 2.1, according to the difference in peak profiles between the initial chromatographic curves of the thin layer plates, the degree of abnormality of the separation effect of any peak on the initial chromatographic curve of any thin layer plate is obtained.

[0063] It should be noted that during the thin layer chromatography detection process, the thin layer plate is required to have uniform thickness and a flat surface. However, in the actual working process, due to the uneven coating of the stationary phase, the stationary phase is unevenly distributed at different positions of the thin layer plate, resulting in different adsorption forces for the components to be tested at different positions, and the support plate coated with the stationary phase itself may have an uneven surface, which makes the retention capacity of the sample at different positions different, resulting in incomplete separation of substances, and differences in the peak shape of the obtained chromatogram, that is, there may be multiple overlapping unseparated peaks, and the chromatographic peak width is also increased to a certain extent compared to normal.

[0064] It should be noted that, in order to analyze whether a certain thin layer plate has a non-uniform surface, this embodiment analyzes the abnormality of the separation effect of each peak by comparing the initial chromatographic curves corresponding to multiple thin layer plates.

[0065] It should be noted that the degree of abnormality in the separation effect of any peak on the initial chromatographic curve corresponding to the thin layer plate is manifested in: the difference in area, peak width and peak value between the peak and the corresponding peaks of other thin layer plates. The more special the area, peak width and peak value of the peak, and the greater the difference between the area, peak width and peak value of the peak and the corresponding peaks of other thin layer plates, the greater the degree of abnormality in the separation effect of the peak.

[0066] It should be noted that the corresponding peak refers to the peak with the same sequence number as the peak on other thin layer plates.

[0067] First, the abnormality degree of any peak in the initial chromatographic curve of any thin layer plate is obtained according to the sum of the differences between the maximum values ​​of the response signals in any corresponding numbered peaks on the initial chromatographic curve of any thin layer plate and the initial chromatographic curves of other thin layer plates.

[0068] As an embodiment, the method for obtaining the abnormality degree of any peak in the initial chromatographic curve of any thin layer plate is:

[0069]

[0070] in, Indicates The initial chromatographic curve of the thin layer plate The abnormality of each peak, represents the number of thin-layer plates, Indicates The initial chromatographic curve of the thin layer plate The maximum value of the response signal in the peak, Indicates The initial chromatographic curve of the thin layer plate The maximum value of the response signal in the peak, represents the absolute value function.

[0071] It should be noted that Indicates The sum of the differences between the initial chromatographic curve of the first thin layer plate and the maximum values ​​of the response signals in any corresponding peaks on the initial chromatographic curves of the remaining thin layer plates represents the The greater the sum of the differences, the greater the abnormality of the peak in the initial chromatographic curve of each thin layer plate.

[0072] Secondly, the degree of abnormality of the separation effect of any peak on the initial chromatographic curve of any thin layer plate is obtained based on the degree of abnormality of any peak in the initial chromatographic curve of any thin layer plate, the area comparison of the peak with the corresponding peaks on other thin layer plates, and the peak width comparison.

[0073] As an embodiment, the method for obtaining the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate is:

[0074]

[0075] in, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks; Indicates The initial chromatographic curve of the thin layer plate The area of ​​the peak, Indicates The initial chromatographic curve of the thin layer plate The abnormality of each peak, Indicates that except The initial chromatographic curves of all thin layer plates The mean of the areas of the peaks; Indicates The initial chromatographic curve of the thin layer plate The peak width of the peak, Indicates that except The initial chromatographic curves of all thin layer plates The mean of the peak widths of the peaks; represents the absolute value function.

[0076] It should be noted that Indicates The area ratio of any corresponding peak of the initial chromatographic curve of a thin layer plate to the initial chromatographic curve of the other thin layer plates. Due to poor separation effect, peak overlap may occur, resulting in the area of ​​abnormal peaks being larger than that of normal peaks. Therefore, the larger the ratio, the greater the degree of abnormal separation effect. Indicates The difference in peak width between the initial chromatographic curve of a thin layer plate and the initial chromatographic curves of the remaining thin layer plates is the difference in peak width between any corresponding peaks. Since the abnormal separation degree increases the peak width, the greater the difference, the greater the abnormal separation effect.

[0077] Step 2.2, according to the difference in the number of peaks on the initial chromatographic curve between any thin layer plate and the remaining thin layer plates, and the difference in the degree of abnormality of the separation effect of all peaks on the initial chromatographic curve between any thin layer plate and the remaining thin layer plates, the degree to which the mobile phase flow rate needs to be adjusted for the thin layer plate is obtained.

[0078] It should be noted that the uneven surface of the thin layer plate will lead to differences in the adsorption capacity and retention capacity of the sample to be tested at different positions of the thin layer plate, resulting in incomplete separation of the sample in the sample to be tested, resulting in abnormal peak shape in the initial chromatographic curve of the response. At this time, the current abnormality can be improved by adjusting the flow rate of the mobile phase to achieve complete sample separation. The mobile phase carries the sample to be tested forward, and the components are separated from each other in the process of the mobile phase flowing through the stationary phase. Reducing the flow rate of the mobile phase can prolong the contact time between the sample and the stationary phase surface, so that the chromatographic effect of the sample is enhanced, thereby improving the separation degree of the sample and realizing the regulation of the response signal-time curve.

[0079] It should be noted that, in this embodiment, the initial chromatographic curves of several thin layer plates are compared to analyze the degree to which the flow rate of the mobile phase needs to be regulated for any thin layer plate.

[0080] It should be noted that the greater the abnormality of any initial chromatographic curve (the greater the possibility that the separation effect is abnormal due to the presence of peaks on any initial chromatographic curve, and the smaller the difference in the possibility of abnormal separation effects due to the presence of peaks corresponding to different initial chromatographic curves), the greater the possibility that the uniformity of the thin layer plate corresponding to the curve is poor, the worse the separation effect for the sample to be tested, and the more it is necessary to adjust the flow rate of the mobile phase.

[0081] First, according to the difference between the possibility of abnormal separation effect between all peaks and the whole on the initial chromatographic curve of any thin layer plate, the fluctuation degree of abnormal separation effect of the peaks on the initial chromatographic curve of any thin layer plate is obtained.

[0082] As an embodiment, a method for obtaining the fluctuation degree of the abnormal degree of the separation effect of the peak on the initial chromatographic curve of any thin layer plate is as follows:

[0083]

[0084] in, Indicates The degree of fluctuation of the abnormal separation effect of the peaks on the initial chromatographic curve of each thin layer plate, Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curve of the thin layer plate, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks, represents the absolute value function.

[0085] It should be noted that Indicates The sum of the differences between all peaks on the initial chromatographic curve of the thin layer plate and the overall probability of abnormal separation effect is expressed as The fluctuation degree of abnormal separation effect of the peaks on the initial chromatographic curve of the thin layer plate. The smaller the value, the more abnormal the peaks are. The more concentrated the possibility of separation anomalies among the peaks in the initial chromatographic curve of a thin layer plate, the more it is necessary to adjust the flow rate of the mobile phase.

[0086] Secondly, the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate is obtained based on the degree of fluctuation of the abnormal degree of separation effect of the peaks on the initial chromatographic curve of any thin layer plate, the difference in the number of peaks on the initial chromatographic curve between thin layer plates, and the difference in the abnormal degree of separation effect of the peaks.

[0087] As an embodiment, the method for obtaining the degree to which the mobile phase flow rate needs to be regulated for any thin layer plate is as follows:

[0088]

[0089] in, Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates that except The average number of peaks on the initial chromatographic curves of all thin layer plates; Indicates The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curve of the thin layer plate, Indicates that except The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curves of all thin layer plates of a thin layer plate; Indicates The degree of fluctuation of the abnormality of the peak separation effect on the initial chromatographic curve of each thin layer plate; Represents the normalization function.

[0090] It should be noted that Indicates The inverse of the ratio of the number of peaks on the initial chromatographic curve of the first thin layer plate to that of the remaining thin layer plates indicates the The normal number of peaks on the initial chromatographic curve of a thin layer plate. The larger the value, the higher the peak value. The fewer the number of peaks on the initial chromatographic curve of the first thin layer plate, the more serious the peak overlap phenomenon is. The greater the degree to which the mobile phase flow rate needs to be controlled for each thin layer plate; Indicates The ratio of the initial chromatographic curve of the first thin layer plate to the abnormal separation effect of the peaks on the remaining thin layer plates shows that The greater the ratio, the more prominent the peak separation effect is on the initial chromatographic curve of the first thin layer plate. The greater the degree to which the mobile phase flow rate needs to be controlled for each thin layer plate.

[0091] Step S003, adjusting the mobile phase flow rate of all thin layer plates according to the degree to which the mobile phase flow rate needs to be adjusted for the thin layer plates, and using the chromatographic curve of any thin layer plate after the mobile phase flow rate adjustment as the compensation curve of the initial chromatographic curve of the thin layer plate.

[0092] It should be noted that the greater the degree to which the thin layer plate needs to control the mobile phase flow rate, the greater the portion of the mobile phase flow rate of the thin layer plate that should be controlled on the original basis. Therefore, the controlled mobile phase flow rate of any thin layer plate can be obtained based on the degree to which the mobile phase flow rate of the thin layer plate needs to be controlled and the mobile phase flow rate of the thin layer plate.

[0093] As an embodiment, the method for obtaining the adjusted mobile phase flow rate of any thin layer plate is as follows:

[0094]

[0095] in, Indicates After adjusting the flow rate of the mobile phase of each thin layer plate, Indicates The mobile phase flow rate of the thin layer plate is To preset the first parameter, Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate.

[0096] The sample to be tested on the thin layer plate is redeveloped using the adjusted flow rate of the mobile phase to obtain a corresponding chromatographic curve, which is recorded as a compensation curve of the initial chromatographic curve of the thin layer plate.

[0097] It should be noted that the first parameter is preset based on experience. It is set to 1 and can be adjusted according to actual conditions. This embodiment does not make any specific limitation.

[0098] As an embodiment, after obtaining the compensation curve of the initial chromatographic curve of the thin layer plate, the abnormal thin layer plate is screened according to the initial chromatographic curve of the thin layer plate and the compensation curve. The specific acquisition method is:

[0099] The difference in the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the mobile phase flow rate is adjusted, and the difference in the abnormal degree of separation effect of all corresponding peaks on the initial chromatographic curve and compensation curve of the thin layer plate before and after the mobile phase flow rate are combined to obtain the effective degree of regulation of any thin layer plate; the difference in the effective degree of regulation between any thin layer plate and all thin layer plates, and the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the regulation are combined to determine the degree to which the thin layer plate needs maintenance, and abnormal thin layer plates are screened according to the degree to which the thin layer plates need maintenance.

[0100] It should be noted that the mobile phase flow rate is regulated in step S002, but there is still a situation where the regulation effect is not good. In this case, it means that the defects of the thin layer plate are serious and the abnormal thin layer plates need to be screened. Therefore, it is also necessary to compare the abnormal degree of separation effect of any peak on the initial chromatographic curve and compensation curve of any thin layer plate and the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated, so as to obtain the effective degree of regulation of any thin layer plate, further determine the degree to which the thin layer plate needs maintenance, and thus screen out abnormal thin layer plates.

[0101] Specifically, in step 3.1, the difference in the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the mobile phase flow rate is adjusted, and the difference in the degree of abnormality of the separation effect of all corresponding peaks on the initial chromatographic curve and compensation curve of the thin layer plate before and after the mobile phase flow rate are combined to obtain the effective degree of regulation of any thin layer plate.

[0102] It should be noted that, after the flow rate of the mobile phase is adjusted, the poor separation effect of some thin layer plates caused by the unevenness of the thin layer plates is significantly improved, and even the samples can be completely separated. That is, the abnormality can be improved by prolonging the contact time between the samples and the stationary phase. However, there are also cases where the separation effect does not change much or is not significantly improved after adjusting the flow rate of the mobile phase. At this time, the current defect cannot be improved by adjusting the contact time between the samples and the stationary phase, which means that the corresponding thin layer plates are more likely to be severely uneven and human intervention is required (timely maintenance or replacement of the corresponding thin layer plates).

[0103] It should be noted that, in this embodiment, the thin layer plate with poor regulation effect is determined by analyzing the difference in separation effect on the test sample before and after regulating the mobile phase flow rate of the thin layer plate. If the difference in separation effect before and after regulation is small, and the separation effect after regulation is poor, the possibility that the corresponding thin layer plate is a thin layer plate with poor regulation effect is greater.

[0104] First, obtain the The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate.

[0105] As an embodiment, a method for obtaining the degree of change of the degree to which the flow rate of the mobile phase needs to be regulated for any thin layer plate is as follows:

[0106]

[0107] in, Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates that after regulation The degree to which the mobile phase flow rate needs to be controlled for each thin layer plate; represents the absolute value function.

[0108] It should be noted that Indicates the regulation before and after The degree of change in the degree to which the flow rate of the mobile phase needs to be regulated for each thin layer plate, the greater the degree of change, the better the regulation effect, and the greater the regulation effectiveness.

[0109] Secondly, the regulation effectiveness of the thin layer plate is obtained based on the degree of change in the degree to which the mobile phase flow rate needs to be regulated for any thin layer plate and the difference in the degree of abnormality of the separation effect of each corresponding peak on the initial chromatographic curve and the compensation curve of any thin layer plate.

[0110] As an embodiment, the method for obtaining the control effectiveness of any thin layer plate is:

[0111]

[0112] in, Indicates The effectiveness of the regulation of each thin layer plate; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate; Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks, Indicates The compensation curve of the initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks; Indicates The average value of the abnormal degree of separation effect of all peaks on the compensation curve of the initial chromatographic curve of the thin layer plate; represents the absolute value function.

[0113] It should be noted that Indicates The sum of the differences in the abnormal degree of separation effect of each corresponding peak on the initial chromatographic curve and the compensation curve of each thin layer plate, It represents the ratio of the sum of the differences before and after regulation to the abnormal degree of separation effect of all peaks after regulation. The larger the ratio is, the smaller the abnormal degree of separation effect of all peaks on the compensation curve of the initial chromatographic curve is, and therefore the better the regulation effect is.

[0114] Step 3.2, the difference in the effective degree of regulation between any thin layer plate and all thin layer plates, and the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated before and after regulation are integrated to determine the degree to which the thin layer plate needs maintenance, and abnormal thin layer plates are screened according to the degree to which the thin layer plate needs maintenance.

[0115] It should be noted that for any thin layer plate, the worse the regulation effectiveness of the thin layer plate, the greater the degree of maintenance required for the thin layer plate; the greater the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated before and after regulation, the greater the degree of maintenance required for the thin layer plate.

[0116] As an example, the method for obtaining the degree to which any thin layer plate requires maintenance is as follows:

[0117]

[0118] in, Indicates The degree to which the laminate requires maintenance; Indicates The effectiveness of the regulation of the thin layer plate, It represents the mean value of the regulation effectiveness of all thin layer plates; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates that after regulation The degree to which the mobile phase flow rate needs to be controlled for each thin layer plate; represents the absolute value function, Represents the normalization function.

[0119] It should be noted that Indicates The absolute value of the difference between the control effectiveness of a thin layer plate and the mean of all thin layer plates, Indicates The difference between the regulation effectiveness of the first thin layer plate and all thin layer plates is The ratio of the control effectiveness of the thin layer plates, the larger the ratio, the greater the The less effective the regulation of a thin layer of board is, the greater the degree of maintenance required.

[0120] A thin layer plate whose maintenance requirement is greater than a preset second parameter is recorded as an abnormal thin layer plate.

[0121] It should be noted that the preset second parameter is set to 0.5 based on experience, and can be adjusted according to actual conditions, and is not specifically limited in this embodiment.

[0122] As another embodiment, the method for obtaining the abnormal thin layer plate may also be:

[0123] The difference between the Pearson correlation coefficient of the initial chromatographic curve and the compensation curve of the thin layer plate is taken, and the absolute value is taken as the difference between the Pearson correlation coefficient of the initial chromatographic curve and the compensation curve of the thin layer plate. The thin layer plate with a difference greater than the preset third parameter is recorded as an abnormal thin layer plate.

[0124] It should be noted that the preset third parameter is set to 0.1 based on experience, and can be adjusted according to actual conditions, and is not specifically limited in this embodiment.

[0125] At this point, an abnormally thin layer plate is obtained through the above method.

[0126] As an embodiment, after obtaining the abnormal thin layer plate, an alarm message is set according to the abnormal thin layer plate.

[0127] It should be noted that the above steps optimize the curve image of the thin layer chromatography scanner by adjusting the mobile phase flow rate, that is, after adjusting the mobile phase flow rate according to the initial chromatographic curve of the original thin layer, the adjusted thin layer plate is scanned with a thin layer chromatography scanner, and a compensation curve of the initial chromatographic curve of the adjusted thin layer plate is drawn through the imaging system to achieve curve compensation, and the abnormal thin layer plate is obtained by analyzing the compensation effect, and an alarm operation is also required for the abnormal thin layer plate.

[0128] Specifically, an alarm is set to sound when an abnormal thin layer plate exists, and information such as the degree of maintenance required and the effectiveness of the regulation of the thin layer plate is output to the staff through the display device, so that the equipment can be replaced or maintained in time; no alarm is given for non-abnormal thin layer plates, and information such as the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated before regulation and the effectiveness of the regulation of the thin layer plate are displayed, so that the staff can understand the specific development of the sample to be tested.

[0129] At this point, this embodiment is completed.

[0130] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A curve image compensation method for a thin layer chromatography scanner, characterized in that: The method comprises the following steps: Obtaining initial chromatographic curves of several thin layer plates, wherein the initial chromatographic curves include a response signal corresponding to each acquisition time within a preset time, and obtaining several peaks of each initial chromatographic curve; According to the difference in the profiles of the peaks between the initial chromatographic curves of the thin layer plates, the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate is obtained; according to the difference in the number of peaks on the initial chromatographic curves of any thin layer plate and other thin layer plates, and the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curves of any thin layer plate and other thin layer plates, the degree to which the flow rate of the mobile phase needs to be adjusted for the thin layer plate is obtained; Regulating the mobile phase flow rate of all thin layer plates according to the degree to which the mobile phase flow rate of the thin layer plates needs to be regulated, and using the chromatographic curve of any thin layer plate after the mobile phase flow rate is regulated as a compensation curve of the initial chromatographic curve of the thin layer plate; The specific method of obtaining the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate according to the difference in peak profiles between the initial chromatographic curves of the thin layer plate includes: Obtaining the abnormality degree of any peak in the initial chromatographic curve of any thin layer plate; The method for obtaining the abnormal degree of separation effect of any peak on the initial chromatographic curve of any thin layer plate is as follows: in, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks; Indicates The initial chromatographic curve of the thin layer plate The area of ​​the peak, Indicates The initial chromatographic curve of the thin layer plate The abnormality of each peak, Indicates that except The initial chromatographic curves of all thin layer plates The mean of the areas of the peaks; Indicates The initial chromatographic curve of the thin layer plate The peak width of the peak, Indicates that except The initial chromatographic curves of all thin layer plates The mean of the peak widths of the peaks; represents the absolute value function.

2. A curve image compensation method for a thin layer chromatography scanner according to claim 1, characterized in that: The specific method for obtaining the abnormality degree of any peak in the initial chromatographic curve of any thin layer plate is: For any peak in the initial chromatographic curve of any thin layer plate, the difference between the maximum response signal of the peak and the corresponding peak in the initial chromatographic curves of all other thin layer plates is calculated and the absolute value is taken, and the absolute values ​​of all the differences are summed up and recorded as the abnormality degree of any peak in the initial chromatographic curve of the thin layer plate; The corresponding peaks are peaks with the same serial numbers on the initial chromatographic curves of different thin layer plates.

3. A curve image compensation method for a thin layer chromatography scanner according to claim 1, characterized in that: The method of obtaining the degree to which the mobile phase flow rate needs to be regulated on the thin layer plate according to the difference in the number of peaks on the initial chromatographic curve between any thin layer plate and the remaining thin layer plates and the difference in the degree of abnormal separation effect of all peaks on the initial chromatographic curve between any thin layer plate and the remaining thin layer plates includes the following specific methods: According to the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curve of any thin layer plate and the remaining thin layer plates, the fluctuation degree of the abnormal degree of separation effect of the peaks on the initial chromatographic curve of the thin layer plate is obtained; The method to obtain the degree of control of mobile phase flow rate for any thin layer plate is: in, Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates that except The average number of peaks on the initial chromatographic curves of all thin layer plates; Indicates The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curve of the thin layer plate, Indicates that except The average value of the abnormal degree of separation effect of all peaks on the initial chromatographic curves of all thin layer plates of a thin layer plate; Indicates The degree of fluctuation of the abnormality of the peak separation effect on the initial chromatographic curve of each thin layer plate; Represents the normalization function.

4. A curve image compensation method for a thin layer chromatography scanner according to claim 3, characterized in that: The specific method for obtaining the fluctuation degree of the abnormal degree of separation effect of the peaks on the initial chromatographic curve of the thin layer plate according to the difference in the abnormal degree of separation effect of all peaks on the initial chromatographic curve of any thin layer plate and the remaining thin layer plates is: The degree of abnormality in separation effect of each peak on the initial chromatographic curve of any thin layer plate is subtracted from the mean value of the degree of abnormality in separation effect of all peaks on the initial chromatographic curve of the thin layer plate and the absolute value is taken. The absolute value of each difference is summed and recorded as the degree of fluctuation of the degree of abnormality in separation effect of the peaks on the initial chromatographic curve of the thin layer plate.

5. A curve image compensation method for a thin layer chromatography scanner according to claim 1, characterized in that: The method of regulating the mobile phase flow rate for all thin layer plates according to the degree to which the mobile phase flow rate needs to be regulated for the thin layer plates includes: The degree to which the mobile phase flow rate needs to be regulated for any thin layer plate is added to the preset first parameter, and the obtained value is multiplied by the mobile phase flow rate of the thin layer plate to obtain the regulated mobile phase flow rate of the thin layer plate.

6. A curve image compensation method for a thin layer chromatography scanner according to claim 1, characterized in that: After obtaining the compensation curve of the initial chromatographic curve of the thin layer plate, the abnormal thin layer plate is screened according to the initial chromatographic curve of the thin layer plate and the compensation curve. The specific method includes: The difference in the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the mobile phase flow rate is adjusted, and the difference in the abnormal degree of separation effect of all corresponding peaks on the initial chromatographic curve and compensation curve of the thin layer plate before and after the mobile phase flow rate are combined to obtain the effective degree of regulation of any thin layer plate; the difference in the effective degree of regulation between any thin layer plate and all thin layer plates, and the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the regulation are combined to determine the degree to which the thin layer plate needs maintenance, and abnormal thin layer plates are screened according to the degree to which the thin layer plates need maintenance.

7. A curve image compensation method for a thin layer chromatography scanner according to claim 6, characterized in that: The difference in the degree to which the mobile phase flow rate of the thin layer plate needs to be adjusted before and after the mobile phase flow rate is adjusted, and the difference in the degree of abnormal separation effect of all corresponding peaks on the initial chromatographic curve and the compensation curve of the thin layer plate before and after the mobile phase flow rate are combined to obtain the effective degree of regulation of any thin layer plate, including the specific method of: Obtain the degree of change in the degree to which the mobile phase flow rate needs to be adjusted for any thin layer plate before and after adjustment; The method for obtaining the control effectiveness of any thin layer plate is: in, Indicates The effectiveness of the regulation of the thin layer plate; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate; Indicates The number of peaks on the initial chromatographic curve of a thin layer plate, Indicates The initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks, Indicates The compensation curve of the initial chromatographic curve of the thin layer plate The degree of abnormal separation of the peaks; Indicates The average value of the abnormal degree of separation effect of all peaks on the compensation curve of the initial chromatographic curve of the thin layer plate; represents the absolute value function.

8. A curve image compensation method for a thin layer chromatography scanner according to claim 7, characterized in that: The specific method for obtaining the degree of change of the degree to which the mobile phase flow rate needs to be regulated by any thin layer plate before and after regulation is: Obtain the abnormal degree of separation effect of any peak on the compensation curve of the initial chromatographic curve of any thin layer plate, and the degree to which the mobile phase flow rate needs to be adjusted after the thin layer plate is adjusted; The difference between the degree to which the mobile phase flow rate needs to be regulated for any thin layer plate and the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate after the regulation is calculated and the absolute value is taken. The absolute value of the difference is compared with the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate after the regulation, and is recorded as the degree of change in the degree to which the mobile phase flow rate needs to be regulated for the thin layer plate.

9. A curve image compensation method for a thin layer chromatography scanner according to claim 6, characterized in that: The method of combining the difference in the effective degree of regulation between any thin layer plate and all thin layer plates and the degree to which the mobile phase flow rate of the thin layer plate needs to be regulated before and after regulation to determine the degree to which the thin layer plate needs maintenance, and screening out abnormal thin layer plates according to the degree to which the thin layer plate needs maintenance, includes the following specific methods: The degree of maintenance required for any thin layer plate can be obtained by: in, Indicates The degree to which the laminate requires maintenance; Indicates The effectiveness of the regulation of the thin layer plate, It represents the mean value of the regulation effectiveness of all thin layer plates; Indicates The degree to which the flow rate of the mobile phase needs to be controlled for each thin layer plate. Indicates that after regulation The degree to which the mobile phase flow rate needs to be controlled for each thin layer plate; represents the absolute value function, represents the normalization function; A thin layer plate whose maintenance requirement is greater than a preset second parameter is recorded as an abnormal thin layer plate.

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