Root box device for researching fungus-mediated photosynthetic carbon and nutrient underground transaction between plants

Abstract

The invention discloses a root box device for fungus-mediated photosynthetic carbon and soil nitrogen and phosphorus underground transaction between plants. The device is a box body device with an open top, and is formed by combining a cuboid three-dimensional frame and a PVC (Polyvinyl Chloride) baffle plate adhered with a nylon net, and water-permeable and air-permeable holes are formed in a back panel and a bottom plate of the three-dimensional frame. The PVC baffle plates have two dimensions, and the middle parts of the PVC baffle plates are hollow. The PVC baffle is hollowed out and pasted with a 35 [mu] m nylon net to form a blocking unit, so that hyphae can pass through, and root systems cannot pass through. The PVC baffles and the cuboid frame are seamlessly pasted together, and the cuboid frame is divided by the PVC baffles to form three root chambers, four air compartments and two marking chambers. Materials used by the device are environment-friendly, low in cost, durable, simple in using method, good in air permeability and water permeability and suitable for related scientific research.

Description

Technical Field

[0001] The invention belongs to the category of ecological plant interactions and relates to a root box device for studying underground transactions of photosynthetic carbon and nutrients between plants mediated by fungi. Background Art

[0002] Dioecious plants are an important part of terrestrial ecosystems. Due to reproductive costs and selection pressures, they have obvious sexual dimorphism in resource absorption, allocation and utilization. Environmental degradation caused by strong interference from human activities seriously affects the survival of dioecious plants. Plant-microbe interactions can alleviate the recovery of plants after adverse stress. At the same time, beneficial bacteria and fungi in the rhizosphere and root system can improve plant water transport, root growth, plant nutrient absorption and photosynthetic carbon assimilation. Arbuscular mycorrhizal fungi can form hyphal networks underground, mediate the synergistic exchange of plant aboveground carbon and soil nitrogen and phosphorus, and are beneficial to plant nutrient absorption. Therefore, soil plant-microbe underground nutrient transactions are the difficulty and hotspot in studying the sex-differentiated nutrient acquisition strategies of dioecious plants.

[0003] Currently reported root boxes are rarely able to distinguish the laws and characteristics of fungus-mediated photosynthetic carbon transfer between plants, and it is difficult to study the characteristics of underground nitrogen and phosphorus transactions between male and female plants. Therefore, the present invention can provide a method for studying the transfer and utilization laws of plant photosynthetic carbon and soil nutrients between male and female plants of the same sex and opposite sex mediated by arbuscular fungi hyphae. Summary of the invention

[0004] The purpose of the present invention is to address the inadequacies of existing root boxes and solve the problems mentioned in the above background technology, and to provide a root box device for studying the transfer, distribution and exchange of photosynthetic carbon and soil nutrients mediated by fungal hyphae between plants of the same sex or plants of the opposite sex.

[0005] A rhizobox device for studying fungal-mediated underground transactions of photosynthetic carbon and nutrients between plants, including: The first root chamber, the first root chamber, the third root chamber; the first air compartment, the second air compartment, the third air compartment, the fourth air compartment; A first marking room and a second marking room; The first root chamber corresponds to the first marking chamber, and the two are separated by a third air compartment to form a first unit; The third root chamber corresponds to the second marking chamber, and the two are separated by a fourth air compartment to form a second unit; A first air compartment is provided between the first unit and the second root chamber; a second air compartment is provided between the second unit and the second root chamber; Each of the air compartments is provided with a barrier unit, one of which allows hyphae to pass through but not roots; and the other barrier unit allows both hyphae and roots to pass through.

[0006] Beneficial effects of the present invention: 1. There are two air compartments between the three root chambers of the device. The PVC baffle of the air compartment of the root box device is pasted with a 35μm nylon mesh. The mycelium can pass through the air compartment, but the roots and root secretions cannot pass through, which can distinguish the contribution of fungi to the underground nutrient transaction of plants.

[0007] 2. There are two marking chambers and air compartments in front of the root chamber of the device. The PVC baffle of the air compartment is pasted with a 35 μm nylon mesh. The air compartment allows mycelium to pass through, but the roots and root secretions cannot pass through. The two marking chambers can be used as nitrogen and phosphorus marking chambers, and the marking chambers 15 N Double-labeled ammonium nitrate was used as the labeled nitrogen source. 33 P-orthophosphate is used as a labeled phosphorus source. Through isotope tracing, it can be used to study the cooperative exchange of photosynthetic carbon transfer between plants and soil nitrogen and phosphorus mediated by fungal hyphae.

[0008] 3. This device is different from the previous two-chamber root box. The three-chamber root box can be well used to study the relationship between different sexes of dioecious plants. Plants will respond to neighboring plants of the same sex and opposite sex at the same time under stress, and better explore the transfer and utilization rules between the same sex and opposite sex of male and female plants. Different root chambers are not only used to grow dioecious plants, but also to grow different plants and explore the mechanism of interaction between different plants.

[0009] 4. Since the device has 5 rows of 6 holes and 1 row of 6 holes of water-permeable and air-permeable holes on the back panel and the bottom panel, it solves the problem of most root boxes being closed and airtight and impermeable. It is beneficial for oxygen to enter the soil pores and the roots to carry out aerobic respiration, and it is also beneficial for the drainage of the root box. There will be no water accumulation and root hypoxia, which inhibits the denitrification of denitrifying bacteria and prevents the loss of soil nitrogen fertilizer.

[0010] 5. The device is easy to disassemble. The PVC can be separated by sticking different nylon meshes, 35 μm nylon mesh (mycelium can pass through, but roots cannot) or 500 μm nylon mesh (mycelium and roots can pass through) to form air compartments with various functions, such as bacteria only, fungi, bacteria and fungi, bacteria, fungi and roots, etc., which can be better used to explore the relationship between soil microorganisms and plants.

[0011] 6. The device is made of environmentally friendly materials, easy to make, simple to use, and has improved water permeability and air permeability. It has low cost, long service life, and can be reused repeatedly. It is suitable for research and teaching of relevant scientific research content in colleges and universities and scientific research institutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the three-dimensional structure of the device of the embodiment of the present application; Figure 2 It is a schematic diagram of a rectangular parallelepiped frame of an embodiment of the present application; Figure 3 It is a schematic diagram of a PVC baffle of the device of the embodiment of the present application with a specification size of 300 mm × 300 mm; Figure 4 It is a schematic diagram of the PVC baffle of the device of the embodiment of the present application with a specification size of 200 mm × 300 mm. DETAILED DESCRIPTION

[0013] The specific embodiments of the present invention are further described in detail with reference to the accompanying drawings.

[0014] like Figure 1 The device of the embodiment of the present application is composed of a rectangular three-dimensional frame 1 with an opening on the top and 8 PVC baffles (baffles 8, 9, 10, 11, 12, 13, 13, 14, 15), the material of which is mainly PVC opaque material. The PVC baffles separate three root chambers, namely root chamber A (first root chamber), root chamber B (second root chamber), and root chamber C (third root chamber), and four air compartments, namely air compartment 2 (first air compartment), air compartment 3 (second air compartment), air compartment 4 (third air compartment), and air compartment 5 (fourth air compartment), and two marking chambers, namely marking chamber 6 (first marking chamber) and marking chamber 7 (second marking chamber), wherein root chamber A (first root chamber) and air compartment 4 (third air compartment) constitute the first unit, and root chamber C and air compartment 5 constitute the second unit.

[0015] Further, the root box device length L1 = 640 mm, the device width L2 = 300 mm, the device height L3 = 300 mm, the rectangular frame thickness L4 = 5 mm, the widths of the four air compartments are all L5 = 20 mm, the widths of the two marking chambers are all L6 = 50 mm, and the aperture of the drainage hole 16 is 4 mm.

[0016] like Figure 2 As shown, in a certain example, the rectangular three-dimensional frame 1 is made of opaque PVC sheet material, the rectangular three-dimensional frame length L1 = 640 mm, the rectangular frame width L2 = 300 mm, the rectangular frame height L3 = 300 mm, the rectangular frame thickness L4 = 5 mm, a front panel, a back panel, two side panels, and a bottom panel.

[0017] Furthermore, the front panel has a size of 640 mm × 300 mm and a thickness of 5 mm; the back panel has a size of 640 mm × 300 mm and a thickness of 5 mm. The back panel has 5 rows of 6 drainage holes in each row, with a hole diameter of 4 mm. The side panels have a size of 300 mm × 300 mm and a thickness of 5 mm, without water-permeable and air-permeable holes. The bottom panel has a size of 640 mm × 300 mm and a thickness of 5 mm. The bottom panel has 2 rows of 3 drainage holes in each row.

[0018] Figure 3 and Figure 4 As shown, in a certain example, there are two sizes of PVC baffles. One size of the PVC baffle is 300 mm × 300 mm, with a hollow in the middle; the other size of the PVC baffle is 200 mm × 300 mm, with a hollow in the middle.

[0019] Figure 3 As shown, the PVC baffle has a size of 300 mm × 300 mm, a thickness of 5 mm, a length of L1 of 300 mm, a length of L2 of 300 mm, a length of L3 of 20 mm, a length of L4 of 70 mm, a length of L5 of 70 mm, a length of L6 of 20 mm, a length of L7 of 120 mm, and a length of L8 of 20 mm.

[0020] Figure 4 As shown, the PVC baffle has a size of 200 mm × 300 mm, a thickness of 5 mm, a length of L1 of 200 mm, a length of L2 of 300 mm, a length of L3 of 20 mm, a length of L4 of 90 mm, a length of L5 of 90 mm, and a length of L6 of 2 mm.

[0021] The design and assembly process of the device of this application is as follows: Four baffles with a size of 300 mm × 300 mm are selected as baffle 8, baffle 9, baffle 10, and baffle 11. The middle hollow of the baffle can be cut into a nylon mesh of suitable size, and a 35 μm nylon mesh (mycelium can pass through, but roots cannot) is pasted on it. The nylon mesh is tightly and seamlessly pasted to the PVC file using super glue.

[0022] After the nylon mesh is pasted, the baffle 8 is tightly pasted to the rectangular three-dimensional frame by heat welding with an electric hot-melt glue stick at 200 mm on the left side of the rectangular three-dimensional frame; the baffle 9 is tightly pasted to the rectangular three-dimensional frame by heat welding with an electric hot-melt glue stick at 200 mm on the right side of the rectangular three-dimensional frame; the baffle 11 is tightly pasted to the rectangular three-dimensional frame by heat welding with an electric hot-melt glue stick at 220 mm on the left side of the rectangular three-dimensional frame; the baffle 10 is tightly pasted to the rectangular three-dimensional frame by heat welding with an electric hot-melt glue stick at 220 mm on the right side of the rectangular three-dimensional frame.

[0023] Four baffles with a size of 200 mm × 300 mm are selected as baffle 12, baffle 13, baffle 14, and baffle 15. The middle hollow of the baffle can be cut into a nylon mesh of suitable size, and a 35 μm nylon mesh (mycelium can pass through, but roots cannot) is pasted on it. The nylon mesh is tightly and seamlessly pasted to the PVC file using super glue.

[0024] The baffle 12 with the nylon mesh pasted on it is located at 70 mm away from the front panel in the A root chamber and pasted on the L4 = 70 mm position of the baffle 8, and is tightly adhered to the rectangular three-dimensional frame by heat welding using an electric hot-melt glue stick. The baffle 14 with the nylon mesh pasted on it is located at 70 mm away from the front panel in the C root chamber and pasted on the L4 = 70 mm position of the baffle 11. The baffle 15 is tightly adhered to the rectangular three-dimensional frame by heat welding using an electric hot-melt glue stick at a distance of 20 mm from the baffle 14.

[0025] After the rectangular frame is pasted, four baffles with a size of 300 mm × 300 mm are formed, namely baffle 8, baffle 9, baffle 10, baffle 11, and four baffles with a size of 200 mm × 300 mm are formed, namely baffle 12, baffle 13, baffle 14, baffle 15. The PVC baffles separate the three root chambers, namely root chamber A, root chamber B, and root chamber C, as well as four air compartments, namely air compartment 2, air compartment 3, air compartment 4, and air compartment 5, and two marking rooms, namely marking chamber 6 and marking chamber 7.

[0026] The use process of the device in this application is as follows: After pasting, the root box can be placed for three days before use. First, fill the root chamber A, root chamber B, root chamber C, and marking chamber 6 and marking chamber 7 with substrate (soil: quartz sand: vermiculite 1:1:1). Plant dioecious plants in root chamber A, root chamber B, and root chamber C to form different planting patterns. After planting for a period of time, the marking chamber 6 15 N can be labeled with double-labeled ammonium nitrate as the nitrogen source, and the 33P-orthophosphate is used as a labeled phosphorus source labeling chamber. Dioecious plants under different planting patterns grow in the root box test device of the present invention, and finally the roots, stems, leaves and rhizosphere soil of the plants are sampled in time, and the role of soil plant-microorganism underground nutrient transaction is systematically analyzed by means of physiology, biochemistry, isotope ecology and microbial informatics, so as to promote the normal development of research related to the transfer and utilization rules of photosynthetic carbon and soil nutrients mediated by fungal hyphae between the same sex and opposite sex of male and female plants, the function and contribution of fungal hyphae.

[0027] The above specifically describes the basic principles, main features and advantages of the present invention. The present invention is not limited by the above embodiments. For practitioners in this industry, changes and improvements of the present invention are considered to be within the scope of protection of the present invention without departing from the technical principles of the present invention.

Claims

1. A root box device for studying fungi-mediated underground transactions of photosynthetic carbon and nutrients between plants, characterized in that: include: The first root chamber, the first root chamber, the third root chamber; the first air compartment, the second air compartment, the third air compartment, the fourth air compartment; A first marking room and a second marking room; The first root chamber corresponds to the first marking chamber, and the two are separated by a third air compartment to form a first unit; The third root chamber corresponds to the second marking chamber, and the two are separated by a fourth air compartment to form a second unit; A first air compartment is provided between the first unit and the second root chamber; a second air compartment is provided between the second unit and the second root chamber; Each of the air compartments is provided with a barrier unit, one of which allows hyphae to pass through but not roots; and the other barrier unit allows both hyphae and roots to pass through.

2. The root box device for studying fungus-mediated underground transactions of photosynthetic carbon and nutrients between plants according to claim 1, characterized in that: The barrier unit is formed by a hollow PVC baffle plate, and a nylon net is arranged in the hollow.

3. The root box device for studying fungus-mediated underground transactions of photosynthetic carbon and nutrients between plants according to claim 1 or 2, characterized in that: Two labeling chambers are used as nitrogen and phosphorus labeling chambers. 15 N Double-labeled ammonium nitrate was used as the labeled nitrogen source. 33 P-orthophosphate was used as the labeled phosphorus source.

4. The root box device for studying fungus-mediated underground transactions of photosynthetic carbon and nutrients between plants according to claim 3, characterized in that: Isotope tracing is used to study the cooperative exchange of photosynthetically transferred carbon between plants and soil nitrogen and phosphorus mediated by fungal hyphae.

5. The root box device for studying fungus-mediated underground transactions of photosynthetic carbon and nutrients between plants according to claim 1, characterized in that: The bottoms and backs of the first root chamber, the second root chamber and the third root chamber are all provided with water-permeable and air-permeable holes.

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