Culture device and method for tremella strain breeding
By designing an automated Tremella seed culture device, automatic bagging and quantitative unloading are achieved using lifting mechanisms and bag support mechanisms, the problem of inefficient manual bagging in the prior art is solved and the production efficiency of Tremella seeds is improved.
Patent Information
- Application Number
- CN202411644220.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-18
- Publication Date
- 2025-01-03
AI Technical Summary
In the existing Tremella seed culture methods, the bagging process requires manual operation, resulting in high labor costs and low efficiency, limiting the large-scale production of Tremella.
A culture device for selecting and breeding for Tremella seeds that are automatically discharged and bagged with automatic bagging is designed. The lifting mechanism and bag support mechanism are used to carry out quantitative unloading of the medium, which is improved by using the lifting mechanism and the unloading mechanism. Productivity.
It realizes automatic bagging and quantitative unloading of Tremella species, saves manpower, improves production efficiency, and solves the problem of inefficient bagging of manual bagging.
Smart Images
Figure CN119234623A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of bacterial strain cultivation, and in particular relates to a cultivation device and method for breeding tremella bacterial strains. Background Art
[0002] Tremella, also known as white fungus, is a nutritious edible fungus with medicinal value. Tremella is rich in nutrition, rich in gelatin, multiple vitamins, 17 kinds of amino acids and glycogen, and has the effects of nourishing yin and moistening the lungs, clearing away heat and moistening dryness, tonifying the spleen and appetizing, and replenishing qi and clearing the intestines. It is known as the "king of fungi". The selection and breeding of Tremella strains is the key link to ensure its excellent traits, increase yield and improve quality.
[0003] The method for cultivating Tremella fuciformis strains includes ingredients preparation, bagging, sterilization, inoculation, mycelium cultivation, ear management, etc. Among them, in the bagging process, manual bagging is required, which requires a lot of manpower, has the disadvantages of high labor cost and low efficiency, and is not conducive to the batch and large-scale production of Tremella fuciformis. Summary of the invention
[0004] The technical problem to be solved by the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a culturing device and method for Tremella fuciformis strain selection and breeding with automatic quantitative unloading, automatic bagging, good cultivation effect and high production efficiency.
[0005] The technical solution adopted to solve the above technical problems is: a plurality of lower support columns are arranged on the inner bottom plate of the incubator, each of the lower support columns is fixedly connected to one end of the upper support column through a third fixed plate, and the other ends of the plurality of upper support columns are fixedly connected to the inner top plate of the incubator respectively, a first support frame is arranged on the top of the plurality of upper support columns, a sleeve is arranged on the first support frame, a feed barrel for storing culture medium is slidably connected in the sleeve, an upper sliding plate is slidably connected to the plurality of upper support columns in a vertical direction, the upper sliding plate is fixedly connected to the feed barrel, a discharging mechanism for discharging the culture medium in the feed barrel is arranged on the upper sliding plate, a plurality of lower support columns are slidably connected to the lower sliding plate in a vertical direction, and the lower sliding plate The plate is located below the upper sliding plate, and a plurality of clamping plates are evenly arranged on the lower sliding plate in the circumferential direction. A culture dish located below the feed tube is arranged inside the plurality of clamping plates, and a cultivation bag for bagging the culture medium is placed inside the culture dish. A lower support plate located above the lower sliding plate is rotatably mounted on the third fixed plate, and a plurality of bag-holding mechanisms for holding open the cultivation bag are arranged on the lower support plate. A first interference block is arranged in the circumferential direction at the bottom of the lower support plate, and a plurality of second interference wheels that interfere with the first interference block are rotatably mounted on the lower sliding plate. An upper support plate located above the lower support plate is fixedly mounted on the third fixed plate, and the upper support plate is located below the upper sliding plate. A second interference block that interferes with the unloading mechanism is arranged on the third fixed plate.
[0006] Furthermore, door panels are provided on the left and right sides of the incubator, and second tracks are provided on both sides of the front opening of the incubator. The two second tracks are slidably connected with baffles in the vertical direction, and the baffles are fixedly connected to the top of the incubator by bolts.
[0007] Furthermore, a plurality of second support frames are arranged on the inner bottom plate of the incubator, each second support frame is respectively provided with a water spray pipe for spraying water, and a heater for heating the culture dish is arranged at the bottom of the lower sliding plate.
[0008] Furthermore, the second contact block is processed with a first inclined surface that contacts the discharge mechanism, and the bottom of the first contact block is processed with a second inclined surface that contacts the second contact wheel.
[0009] Furthermore, the incubator is provided with a lifting mechanism inside for driving an upper sliding plate to move in a vertical direction on a plurality of upper support columns and a lower sliding plate to move in a vertical direction on a plurality of lower support columns, the sliding directions of the upper sliding plate and the lower sliding plate are opposite, and the lifting mechanism is as follows: the upper sliding plate is fixedly connected to the second connecting plate, the inner bottom plate of the incubator is provided with an electric cylinder, the output end of the electric cylinder is fixedly connected to the second connecting plate, the second connecting plate is provided with a second rack meshing with a gear, the gear is rotatably connected to the inner side wall of the incubator through a connecting shaft, the lower sliding plate is fixedly connected to the first connecting plate, the first connecting plate is provided with a first rack meshing with a gear, and the movement direction of the second connecting plate in the vertical direction is opposite to the movement direction of the first connecting plate in the vertical direction.
[0010] The cam is connected with the second sliding plate by the second sliding hole, and the second sliding plate is connected with the second sliding plate by the second sliding hole. The cam is connected to the second sliding block by the spring, and the spring has two ends respectively connected to the first sliding block and the second sliding block having the first and second sliding holes respectively.
[0011] Furthermore, the bag-holding mechanism is as follows: the lower support plate is rotatably connected to one end of the third connecting rod, the other end of the third connecting rod is rotatably connected to the hook, multiple hooks hold open the opening of the cultivation bag, the hook is fixedly connected to the fourth slider, the upper support plate is evenly processed with fifth sliding holes in the circumferential direction, the fourth slider is provided with a third slider slidably connected to the fifth sliding hole, the third fixed plate is provided with a second fixed rod slidably connected to the fourth slider in the horizontal direction, the second fixed rod is provided with a second spring, one end of the second spring is fixedly connected to the third fixed plate, and the other end of the second spring is fixedly connected to the fourth slider.
[0012] A cultivation method using a cultivation device for Tremella fuciformis strain selection and breeding comprises the following steps:
[0013] S1, putting Tremella culture medium into a feed barrel: weigh 25-35 parts of Ganoderma lucidum extraction residue, 25-35 parts of lotus seed shell, 10-20 parts of cottonseed shell, 15-25 parts of bran, and 0.5-1.5 parts of gypsum by mass percentage, stir evenly to obtain a culture medium with a water content of 55-60%, put the culture medium into the feed barrel, and the culture medium in the feed barrel falls onto two lower discharge plates;
[0014] S2, automatically bagging the culture medium in the feed barrel into the cultivation bag: the lifting mechanism drives the feed barrel on the upper sliding plate to move downward in the vertical direction on the multiple upper support columns, and the lifting mechanism drives the culture dish on the lower sliding plate to move upward in the vertical direction on the multiple lower support columns. The movement direction of the upper sliding plate is opposite to that of the lower sliding plate. When the lower sliding plate drives the culture dish and the cultivation bag to move upward, the bag-supporting mechanism opens the opening of the cultivation bag, and the outlet end of the feed barrel corresponds to the opening end of the cultivation bag. The unloading mechanism on the upper sliding plate opens the opening end of the connecting barrel, and the culture medium in the feed barrel enters the cultivation bag. After bagging, the cultivation bag is manually tied;
[0015] S3, sterilization, inoculation, mycelium cultivation: the cultivation bag containing the culture medium is taken out and placed in a high-pressure steam sterilizer, the temperature is raised to 121°C and maintained for 1.5 hours, the sterilized culture medium is cooled to below 30°C, inoculated in a sterile inoculation room, the cultivation bag containing the culture medium is continued to be put back into the culture dish, the humidity is controlled to 60-70% by spraying water through a water spray pipe, the temperature is controlled to 20-25°C by a heater, and cultured for 45 days. After the culture medium in the cultivation bag is penetrated with mycelium and ear buds are formed, the bag mouth of the cultivation bag 21 is opened, the temperature is controlled to 20-23°C, the relative humidity is maintained at 80-85%, and when the ear pieces are stretched, the relative humidity is 85-90%;
[0016] S4, breeding of Tremella fuciformis strains: breeding of Tremella fuciformis strains by manually observing the Tremella fuciformis in the incubator.
[0017] Furthermore, in the step S2, the lifting mechanism drives the feed barrel on the upper sliding plate to move downward in the vertical direction on multiple upper support columns, and the lifting mechanism drives the culture dish on the lower sliding plate to move upward in the vertical direction on multiple lower support columns. The method is: the output end of the electric cylinder drives the second connecting plate to move in the vertical direction, the second connecting plate drives the upper sliding plate to move downward in the vertical direction on multiple upper support columns, the second rack on the second connecting plate drives the gear to rotate by meshing with the gear, the gear drives the first connecting plate to move in the vertical direction by meshing with the first rack on the first connecting plate, and the first connecting plate drives the lower sliding plate to move upward in the vertical direction on multiple lower support columns.
[0018] Furthermore, the method for the bag-supporting mechanism in step S2 to open the opening of the cultivation bag is as follows: during the upward movement of the lower sliding plate in the vertical direction, the second interference wheel on the lower sliding plate interferes with the second inclined surface of the first interference block at the bottom of the lower support plate, the first interference block drives the lower support plate to rotate in the third fixed plate, the lower support plate drives the hook to move through the third connecting rod, the hook drives the fourth slider to slide in the horizontal direction on the second fixed rod, the third slider on the fourth slider slides in the fifth sliding hole of the upper support plate, and the hook supports the opening of the cultivation bag;
[0019] The camming mechanism of the two wheels is actuated by the second camming wheel, and the second camming wheel is adapted to move upwardly from the first track to the second track, the two wheels being adapted to move upwardly from the first track to the second track.
[0020] The beneficial effects of the present invention are as follows: (1) The present invention adopts a lower sliding plate to drive the culture dish and the cultivation bag to move upward, and the bag-supporting mechanism opens the opening of the cultivation bag, the outlet end of the feed tube corresponds to the opening end of the cultivation bag, and the unloading mechanism on the upper sliding plate opens the opening end of the connecting tube, and the culture medium of the feed tube enters the cultivation bag, which can be automatically unloaded and automatically bagged, which is convenient for the selection and breeding of Tremella fuciformis strains. The present invention solves the problem of low efficiency of manual bagging, saves manpower, and improves production efficiency.
[0021] (2) The present invention adopts a method in which the upper sliding plate moves downward in the vertical direction, and the first contact wheels on both sides of the connecting frame respectively contact with the first inclined surface of the second contact block, each connecting frame respectively drives the lower unloading plate to move, and the two lower unloading plates are separated. The third sliding rod of each lower unloading plate respectively drives the second connecting rod to rotate, and each second sliding rod respectively drives the upper unloading plate to slide in the horizontal direction in the first fixed plate. The two upper unloading plates are combined to block the internal opening end of the feed barrel, so that the culture medium can be quantitatively unloaded. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1It is a structural schematic diagram of an embodiment of a culture device for selecting and breeding Tremella fuciformis strains of the present invention.
[0023] Figure 2 yes Figure 1 Schematic diagram of the structure from another angle.
[0024] Figure 3 It is a schematic diagram of the structure in which the culture medium in the feed cylinder is bagged in the cultivation bag.
[0025] Figure 4 It is a schematic diagram of the structure in which the feed barrel and the cultivation bag are separated.
[0026] Figure 5 It is a structural diagram of the lifting mechanism.
[0027] Figure 6 yes Figure 5 Schematic diagram of the structure from another angle.
[0028] Figure 7 yes Figure 3 Schematic diagram of the structure of the upper sliding plate, upper support plate, lower support plate and lower sliding plate.
[0029] Figure 8 It is a schematic diagram of the structure of the lower sliding plate, the culture dish and the second friction wheel.
[0030] Fig. 9 It is a schematic diagram of the bottom structure of the lower sliding plate.
[0031] Fig.10 It is a structural schematic diagram of the second abutment block on the upper support plate and the first abutment block on the lower support plate.
[0032] Fig.11 It is a structural schematic diagram of the lower support plate.
[0033] Fig.12 It is a structural diagram of the unloading mechanism.
[0034] Fig.13 It is a schematic diagram of the parts structure on the upper unloading plate and the lower unloading plate.
[0035] Fig.14 It is a structural schematic diagram of the first fixing plate, the second fixing plate and the connecting tube.
[0036] Fig.15 It is a schematic diagram of the parts structure on the upper support plate and the lower support plate.
[0037] Fig.16 It is a structural diagram of the bag-supporting mechanism.
[0038] Fig.17 It is a structural schematic diagram of a third connecting rod, a hook, a fourth sliding block and a second fixing rod.
[0039] 1. Feeding barrel; 2. Lifting mechanism; 201. First rack; 202. First connecting plate; 203. Gear; 204. Second connecting plate; 205. Electric cylinder; 206. Connecting shaft; 207. Second rack; 3. Discharging mechanism; 301. Upper discharging plate; 302. First fixed plate; 303. First sliding hole; 304. First abutting wheel; 305. Connecting frame; 306. First connecting rod; 307. First track; 308. First sliding block; 309. Second fixed plate; 310. First sliding bar; 311. Second sliding bar; 312. Second sliding hole; 313. Second connecting rod; 314. Third sliding hole; 315. Third sliding bar; 316. Lower discharging plate; 317. First fixed rod; 318. First spring; 319. Connecting ring; 320 , second slider; 321, fourth slide hole; 322, connecting tube; 4, bag support mechanism; 401, third slider; 402, fifth slide hole; 403, third connecting rod; 404, hook; 405, fourth slider; 406, second fixing rod; 407, second spring; 5, door panel; 6, incubator; 7, second track; 8, shielding plate; 9, bolt; 10, first support frame; 11, water spray pipe; 12, second support frame; 13, upper support column; 14, upper sliding plate; 15, upper support plate; 16, lower support plate; 17, lower sliding plate; 18, culture dish; 19, clamping plate; 20, second contact wheel; 21, cultivation bag; 22, heater; 23, first contact block; 24, second contact block; 25, sleeve; 26, third fixing plate; 27, lower support column. DETAILED DESCRIPTION
[0040] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0041] like Figures 1 to 4 , Figures 7 to 11 As shown, the culture device for Tremella fuciformis strain selection and breeding of the present embodiment is composed of a feed barrel 1, a lifting mechanism 2, a unloading mechanism 3, a bag-supporting mechanism 4, a door panel 5, a culture box 6, a second track 7, a baffle plate 8, a bolt 9, a first support frame 10, a water spray pipe 11, a second support frame 12, an upper support column 13, an upper sliding plate 14, an upper support plate 15, a lower support plate 16, a lower sliding plate 17, a culture dish 18, a clamping plate 19, a second contact wheel 20, a cultivation bag 21, a heater 22, a first contact block 23, a second contact block 24, a sleeve 25, a third fixed plate 26, and a lower support column 27.
[0042] Door panels 5 are provided on the left and right sides of the incubator 6, and second rails 7 are provided on both sides of the front opening of the incubator 6. The two second rails 7 are slidably connected with shielding plates 8 in the vertical direction, and the shielding plates 8 are fixedly connected to the top of the incubator 6 by bolts 9. A plurality of lower support columns 27 are provided on the inner bottom plate of the incubator 6, and each lower support column 27 is fixedly connected to one end of the upper support column 13 through a third fixing plate 26, and the other ends of the plurality of upper support columns 13 are fixedly connected to the inner top plate of the incubator 6, respectively. A first support frame 10 is provided on the top of the plurality of upper support columns 13, and a sleeve 25 is provided on the first support frame 10, and a feed barrel 1 for storing culture medium is slidably connected in the sleeve 25, and an upper sliding plate 14 is slidably connected to the plurality of upper support columns 13 in the vertical direction, and the upper sliding plate 14 is fixedly connected to the feed barrel 1, and a discharge mechanism 3 for discharging the culture medium in the feed barrel 1 is provided on the upper sliding plate 14, and a plurality of lower support columns 27 are slidably connected with lower sliding plates in the vertical direction. Plate 17, the lower sliding plate 17 is located below the upper sliding plate 14, a plurality of card plates 19 are evenly arranged on the circumferential direction of the lower sliding plate 17, a culture dish 18 located below the feed tube 1 is arranged inside the plurality of card plates 19, a cultivation bag 21 for bagging the culture medium is placed inside the culture dish 18, a lower support plate 16 located above the lower sliding plate 17 is rotatably mounted on the third fixed plate 26, a plurality of bag-holding mechanisms 4 for holding open the cultivation bag 21 are arranged on the lower support plate 16, a first interference block 23 is arranged on the circumferential direction of the bottom of the lower support plate 16, a plurality of second interference wheels 20 that interfere with the first interference block 23 are rotatably mounted on the lower sliding plate 17, and a second inclined surface that interferes with the second interference wheel 20 is processed at the bottom of the first interference block 23. The upper support plate 15 located above the lower support plate 16 is fixedly mounted on the third fixed plate 26, and the upper support plate 15 is located below the upper sliding plate 14. The third fixed plate 26 is provided with a second abutment block 24 abutting against the discharge mechanism 3, and the second abutment block 24 is processed with a first inclined surface abutting against the discharge mechanism 3. A plurality of second support frames 12 are provided on the inner bottom plate of the incubator 6, and each second support frame 12 is provided with a water spraying pipe 11 for spraying water, and a heater 22 for heating the culture dish 18 is provided at the bottom of the lower sliding plate 17.
[0043] like Figure 5 to Figure 6 As shown, a lifting mechanism 2 is provided inside the incubator 6 for driving the upper sliding plate 14 to move in a vertical direction on a plurality of upper support columns 13 and the lower sliding plate 17 to move in a vertical direction on a plurality of lower support columns 27. The sliding directions of the upper sliding plate 14 and the lower sliding plate 17 are opposite. The lifting mechanism 2 is composed of a first rack 201, a first connecting plate 202, a gear 203, a second connecting plate 204, an electric cylinder 205, a connecting shaft 206, and a second rack 207.
[0044] The lifting mechanism 2 is as follows: the upper sliding plate 14 is fixedly connected to the second connecting plate 204, the inner bottom plate of the incubator 6 is provided with an electric cylinder 205, the output end of the electric cylinder 205 is fixedly connected to the second connecting plate 204, the second connecting plate 204 is provided with a second rack 207 meshing with the gear 203, the gear 203 is fixedly connected to the connecting shaft 206, the connecting shaft 206 is rotatably connected to the inner wall of the incubator 6, the lower sliding plate 17 is fixedly connected to the first connecting plate 202, the first connecting plate 202 is provided with a first rack 201 meshing with the gear 203, and the movement direction of the second connecting plate 204 along the vertical direction is opposite to the movement direction of the first connecting plate 202 along the vertical direction.
[0045] like Figure 12 to Figure 14 As shown, the unloading mechanism 3 is composed of an upper unloading plate 301, a first fixed plate 302, a first sliding hole 303, a first contact wheel 304, a connecting frame 305, a first connecting rod 306, a first track 307, a first slider 308, a second fixed plate 309, a first sliding rod 310, a second sliding rod 311, a second sliding hole 312, a second connecting rod 313, a third sliding hole 314, a third sliding rod 315, a lower unloading plate 316, a first fixed rod 317, a first spring 318, a connecting ring 319, a second slider 320, a fourth sliding hole 321, and a connecting tube 322.
[0046] The unloading mechanism 3 is as follows: a second fixed plate 309 is provided on the upper sliding plate 14, and the second fixed plate 309 is fixedly connected to the first fixed plate 302 through a connecting tube 322. The first fixed plate 302 is located above the second fixed plate 309, and the second fixed plate 309 has connecting frames 305 on both sides thereof slidingly connected in the horizontal direction, each connecting frame 305 is respectively provided with a first contact wheel 304 that contacts with the first inclined surface of the second contact block 24, and each connecting frame 305 is respectively fixedly connected to a lower unloading plate 316, and the two lower unloading plates 316 are located inside the connecting tube 322. Fourth sliding holes 321 are respectively processed on both sides of the second fixed plate 309, and each lower unloading plate 316 is respectively provided with a first contact wheel 304 that contacts with the first inclined surface of the second contact block 24. A second slider 320 is slidably connected, and both sides of each connecting frame 305 are rotatably connected to one end of the first connecting rod 306. First tracks 307 are respectively arranged on both sides of the upper sliding plate 14. Each first track 307 is respectively slidably connected to the first slider 308 in the horizontal direction. The other end of each first connecting rod 306 is respectively rotatably connected to the first slider 308. First fixed rods 317 slidably connected to the first slider 308 in the horizontal direction are respectively arranged on both sides of the second fixed plate 309. Each first fixed rod 317 is respectively arranged with a first spring 318. One end of each first spring 318 is respectively fixedly connected to the second fixed plate 309, and the other end of each first spring 318 is respectively fixedly connected to the first slider 308.
[0047] A connecting ring 319 is provided on the connecting cylinder 322, and both sides of the connecting ring 319 are rotatably connected to the second connecting rod 313 respectively. A third sliding hole 314 is processed at the bottom of each second connecting rod 313, and a second sliding hole 312 is processed at the top of each second connecting rod 313. A third sliding rod 315 slidably connected to the third sliding hole 314 is provided on each second sliding block 320. First sliding holes 303 are processed at both sides of the first fixed plate 302. Upper and lower discharge plates 301 are slidably connected to the first fixed plate 302 on both sides along the horizontal direction. The two upper and lower discharge plates 301 are located inside the feeding cylinder 1, and the two upper and lower discharge plates 301 are located above the two lower and lower discharge plates 316. A first sliding rod 310 slidably connected to the first sliding hole 303 along the horizontal direction is processed on each upper and lower discharge plate 301, and a second sliding rod 311 slidably connected to the second sliding hole 312 is provided at the bottom of each upper and lower discharge plate 301.
[0048] like Figures 15 to 17 As shown, the bag-supporting mechanism 4 is composed of a third slider 401 , a fifth slide hole 402 , a third connecting rod 403 , a hook 404 , a fourth slider 405 , a second fixing rod 406 , and a second spring 407 .
[0049] The bag-supporting mechanism 4 comprises: a lower support plate 16 is rotatably connected to one end of a third connecting rod 403, the other end of the third connecting rod 403 is rotatably connected to a hook 404, a plurality of hooks 404 prop open the opening of the cultivation bag 21, the hook 404 is fixedly connected to a fourth slider 405, the upper support plate 15 is uniformly processed with fifth sliding holes 402 in the circumferential direction, the fourth slider 405 is provided with a third slider 401 slidably connected to the fifth sliding hole 402, the third fixed plate 26 is provided with a second fixed rod 406 slidably connected to the fourth slider 405 in a horizontal direction, the second fixed rod 406 is provided with a second spring 407, one end of the second spring 407 is fixedly connected to the third fixed plate 26, and the other end of the second spring 407 is fixedly connected to the fourth slider 405.
[0050] Example 1
[0051] The culturing method of a tremella fungus strain selection and breeding culturing device of the present embodiment comprises the following steps:
[0052] S1, put the Tremella culture medium into the feed barrel 1: weigh 32 parts of Ganoderma lucidum extraction residue, 32 parts of lotus seed shells, 15 parts of cottonseed shells, 20 parts of bran, and 1 part of gypsum by mass percentage, stir evenly to obtain a culture medium with a water content of 55-60%, put the culture medium into the feed barrel 1, and the culture medium in the feed barrel 1 falls onto the two lower discharge plates 316.
[0053] S2, the culture medium in the feed barrel 1 is automatically bagged in the cultivation bag 21: the lifting mechanism 2 drives the feed barrel 1 on the upper sliding plate 14 to move downward in the vertical direction on the multiple upper support columns 13, and the lifting mechanism 2 drives the culture dish 18 on the lower sliding plate 17 to move upward in the vertical direction on the multiple lower support columns 27. The movement direction of the upper sliding plate 14 is opposite to the movement direction of the lower sliding plate 17. During the upward movement of the culture dish 18 and the cultivation bag 21 driven by the lower sliding plate 17, the bag-supporting mechanism 4 opens the opening of the cultivation bag 21, and the outlet end of the feed barrel 1 corresponds to the opening end of the cultivation bag 21. The unloading mechanism 3 on the upper sliding plate 14 opens the opening end of the connecting tube 322, and the culture medium of the feed barrel 1 enters the cultivation bag 21. After bagging, the cultivation bag 21 is manually tied.
[0054] Among them, the lifting mechanism 2 drives the feeding barrel 1 on the upper sliding plate 14 to move downward in the vertical direction on multiple upper support columns 13, and the lifting mechanism 2 drives the culture dish 18 on the lower sliding plate 17 to move upward in the vertical direction on multiple lower support columns 27. The method is: the output end of the electric cylinder 205 drives the second connecting plate 204 to move in the vertical direction, the second connecting plate 204 drives the upper sliding plate 14 to move downward in the vertical direction on multiple upper support columns 13, the second rack 207 on the second connecting plate 204 drives the gear 203 to rotate by meshing with the gear 203, the gear 203 drives the first connecting plate 202 to move in the vertical direction by meshing with the first rack 201 on the first connecting plate 202, and the first connecting plate 202 drives the lower sliding plate 17 to move upward in the vertical direction on multiple lower support columns 27.
[0055] Among them, the method for the bag-holding mechanism 4 to hold open the opening of the cultivation bag 21 is as follows: when the lower sliding plate 17 moves upward in the vertical direction, the second contact wheel 20 on the lower sliding plate 17 contacts the second inclined surface of the first contact block 23 at the bottom of the lower support plate 16, and the first contact block 23 drives the lower support plate 16 to rotate in the third fixed plate 26, and the lower support plate 16 drives the hook 404 to move through the third connecting rod 403, and the hook 404 drives the fourth slider 405 to slide on the second fixed rod 406 in the horizontal direction, and the third slider 401 on the fourth slider 405 slides in the fifth sliding hole 402 of the upper support plate 15, and the hook 404 holds open the opening of the cultivation bag 21.
[0056] The method by which the discharge mechanism 3 on the upper sliding plate 14 opens the open end of the feed barrel 1 is as follows: during the downward movement of the upper sliding plate 14 in the vertical direction, the first abutting wheels 304 on both sides of the connecting frame 305 abut against the first inclined surfaces of the second abutting blocks 24 respectively, and each connecting frame 305 slides in the horizontal direction in the second fixed plate 309 respectively, and each connecting frame 305 drives the lower discharge plate 316 to move respectively, and each connecting frame 305 drives the first slider 308 to move in the horizontal direction on the first rail 307 and the first fixed rod 317 respectively through the first connecting rod 306, and the two lower discharge plates 316 are separated, and the second slider 32 on each lower discharge plate 316 0 respectively slide in the fourth slide hole 321 of the second fixed plate 309, the third slide bar 315 of each lower unloading plate 316 respectively slides in the third slide hole 314 of the second connecting rod 313 and drives the second connecting rod 313 to rotate, the second slide hole 312 on each second connecting rod 313 slides with the second slide bar 311 at the bottom of the upper unloading plate 301 respectively, each second slide bar 311 respectively drives the upper unloading plate 301 to slide in the first fixed plate 302 along the horizontal direction, the first slide bar 310 of each upper unloading plate 301 respectively slides in the first slide hole 303 of the first fixed plate 302, and the two upper unloading plates 301 are combined to block the inner opening end of the feeding barrel 1.
[0057] S3, sterilization, inoculation, and mycelium cultivation: the cultivation bag 21 containing the culture medium is taken out and placed in a high-pressure steam sterilizer, the temperature is raised to 121° C. and maintained for 1.5 hours, the sterilized culture medium is cooled to below 30° C., and inoculated in a sterile inoculation room, the cultivation bag 21 containing the culture medium is continued to be placed back into the culture dish 18, the humidity is controlled to 60-70% by spraying water through the water spray pipe 11, and the temperature is controlled to 20-25° C. by the heater 22. After 45 days of cultivation, after the culture medium in the cultivation bag 21 has grown mycelium and ear buds have formed, the bag mouth of the cultivation bag 21 is opened, the temperature is controlled to 20-23° C., and the relative humidity is maintained at 80-85%. When the ear pieces begin to stretch, the relative humidity is 85-90%.
[0058] S4, breeding Tremella fuciformis strains: breeding Tremella fuciformis strains by manually observing the Tremella fuciformis in the incubator 6 .
[0059] Embodiment 2:
[0060] Raw material composition: 64 parts of cottonseed hulls, 20 parts of ganoderma lucidum extraction residues, 15 parts of bran, and 1 part of gypsum are taken by mass percentage. The other steps are the same as those in Example 1.
[0061] Embodiment 3:
[0062] Raw material composition: 64 parts of cottonseed hulls, 20 parts of lotus seed hulls, 15 parts of bran, and 1 part of gypsum are taken by mass percentage, and the other steps are the same as those in Example 1.
[0063] Comparative Example:
[0064] Raw material composition: 84 parts of cottonseed hulls, 15 parts of bran, and 1 part of gypsum are taken by mass percentage, and the other steps are the same as those in Example 1.
[0065] Determination of active ingredient content in Tremella fuciformis fruiting body.
[0066] Determination method: The polysaccharide content was determined according to the method for crude polysaccharide content in edible fungi (NY / T1676-2008); the triterpene content was determined according to the revised version of the standard for total triterpene content (NY / SJ339-2001); the polyphenol content was determined according to the internationally commonly used method, the Folin-phenol method, and the polyphenol content was expressed in mg gallic acid / Flammulina velutipes powder.
[0067] The content of active ingredients in Tremella fuciformis fruiting bodies cultivated in different culture media is shown in the following table.
[0068]
[0069] Active substances such as polysaccharides, triterpenes, and polyphenols are key factors in the edible and medicinal value of Tremella fuciformis. According to the results of the above table, the contents of polysaccharides, triterpenes, and polyphenols in Tremella fuciformis fruiting bodies are higher than those in Example 2, Example 3, and the comparative example, wherein the polysaccharide content is 56.22% higher than that in the comparative example, and the triterpenoid content is 27.08% higher than that in the comparative example.
[0070] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.
Claims
1. A culture device for Tremella fuciformis strain selection, characterized in that: A plurality of lower support columns (27) are arranged on the inner bottom plate of the incubator (6), each of the lower support columns (27) is fixedly connected to one end of the upper support column (13) via a third fixing plate (26), the other ends of the plurality of upper support columns (13) are fixedly connected to the inner top plate of the incubator (6), a first support frame (10) is arranged on the top of the plurality of upper support columns (13), a sleeve (25) is arranged on the first support frame (10), a storage device (25) is slidably connected to the sleeve (25) A feed barrel (1) for storing culture medium, a plurality of upper support columns (13) are slidably connected to an upper sliding plate (14) in a vertical direction, the upper sliding plate (14) is fixedly connected to the feed barrel (1), a discharge mechanism (3) for discharging the culture medium in the feed barrel (1) is provided on the upper sliding plate (14), a plurality of lower support columns (27) are slidably connected to a lower sliding plate (17) in a vertical direction, the lower sliding plate (17) is located below the upper sliding plate (14), and the lower sliding plate (17) A plurality of clamping plates (19) are evenly arranged in the circumferential direction of the upper plate; a culture dish (18) located below the feed cylinder (1) is arranged inside the plurality of clamping plates (19); a cultivation bag (21) for bagging the culture medium is placed inside the culture dish (18); a lower support plate (16) located above the lower sliding plate (17) is rotatably mounted on the third fixed plate (26); a plurality of bag-opening mechanisms (4) for opening the cultivation bag (21) are arranged on the lower support plate (16); A first abutment block (23) is arranged in the circumferential direction of the bottom of the support plate (16); a plurality of second abutment wheels (20) abutting against the first abutment block (23) are rotatably mounted on the lower sliding plate (17); an upper support plate (15) located above the lower support plate (16) is fixedly mounted on the third fixed plate (26); the upper support plate (15) is located below the upper sliding plate (14); and a second abutment block (24) abutting against the discharge mechanism (3) is arranged on the third fixed plate (26).
2. The tremella fuciformis strain breeding culture device according to claim 1, characterized in that: Door panels (5) are respectively arranged on the left and right sides of the incubator (6); second rails (7) are respectively arranged on both sides of the front opening of the incubator (6); the two second rails (7) are slidably connected with shielding plates (8) in a vertical direction; and the shielding plates (8) are fixedly connected to the top of the incubator (6) by bolts (9).
3. The culturing device for Tremella fuciformis strain selection and breeding according to claim 1, characterized in that: A plurality of second support frames (12) are arranged on the inner bottom plate of the culture box (6), each second support frame (12) is respectively provided with a water spray pipe (11) for spraying water, and a heater (22) for heating the culture dish (18) is arranged at the bottom of the lower sliding plate (17).
4. The culturing device for Tremella fuciformis strain selection and breeding according to claim 1, characterized in that: The second contact block (24) is machined with a first inclined surface for contacting with the discharge mechanism (3), and the bottom of the first contact block (23) is machined with a second inclined surface for contacting with the second contact wheel (20).
5. The culturing device for Tremella fuciformis strain selection and breeding according to claim 1, characterized in that: The incubator (6) is provided with a lifting mechanism (2) for driving an upper sliding plate (14) to move vertically on a plurality of upper support columns (13) and a lower sliding plate (17) to move vertically on a plurality of lower support columns (27). The sliding directions of the upper sliding plate (14) and the lower sliding plate (17) are opposite. The lifting mechanism (2) comprises: the upper sliding plate (14) is fixedly connected to a second connecting plate (204). The inner bottom plate of the incubator (6) is provided with an electric cylinder (205). The output end of the electric cylinder (205) is connected to the second connecting plate (204). 04), the second connecting plate (204) is provided with a second rack (207) meshing with the gear (203), the gear (203) is rotatably connected to the inner wall of the incubator (6) through a connecting shaft (206), the lower sliding plate (17) is fixedly connected to the first connecting plate (202), the first connecting plate (202) is provided with a first rack (201) meshing with the gear (203), and the movement direction of the second connecting plate (204) along the vertical direction is opposite to the movement direction of the first connecting plate (202) along the vertical direction.
6. The culturing device for Tremella fuciformis strain selection and breeding according to claim 1, characterized in that: The unloading mechanism (3) is as follows: a second fixed plate (309) is arranged on the upper sliding plate (14), the second fixed plate (309) is fixedly connected to the first fixed plate (302) through a connecting tube (322), the first fixed plate (302) is located above the second fixed plate (309), and connecting frames (305) are respectively connected to the second fixed plate (309) on both sides thereof in a horizontally sliding manner, each connecting frame (305) is respectively provided with a first contact wheel (304) contacting with the first inclined surface of the second contact block (24), each connecting frame (305) is respectively fixedly connected to the lower unloading plate (316), the two lower unloading plates (316) are located inside the connecting tube (322), fourth sliding holes (321) are respectively processed on both sides of the second fixed plate (309), and each lower unloading plate (316) is respectively provided with a first contact wheel (304) contacting with the first inclined surface of the second contact block (24). The second slider (320) is slidably connected to the first slider (321), each connecting frame (305) is rotatably connected to one end of the first connecting rod (306) on both sides, first rails (307) are respectively arranged on both sides of the upper sliding plate (14), each first rail (307) is respectively slidably connected to the first slider (308) in the horizontal direction, the other end of each first connecting rod (306) is respectively rotatably connected to the first slider (308), first fixing rods (317) are respectively arranged on both sides of the second fixing plate (309) and are slidably connected to the first slider (308) in the horizontal direction, each first fixing rod (317) is respectively arranged on the first spring (318), one end of each first spring (318) is respectively fixedly connected to the second fixing plate (309), and the other end of each first spring (318) is respectively fixedly connected to the first slider (308); A connecting ring (319) is provided on the connecting cylinder (322), and both sides of the connecting ring (319) are rotatably connected to the second connecting rod (313), and each second connecting rod (313) is respectively processed with a third sliding hole (314) at the bottom, and each second connecting rod (313) is respectively processed with a second sliding hole (312) at the top, and each second sliding block (320) is respectively provided with a third sliding rod (315) slidably connected to the third sliding hole (314), and both sides of the first fixing plate (302) are respectively processed with the first sliding hole (303) ), upper unloading plates (301) are slidably connected to the two sides of the first fixed plate (302) in the horizontal direction, the two upper unloading plates (301) are located inside the feed barrel (1), and the two upper unloading plates (301) are located above the two lower unloading plates (316), each upper unloading plate (301) is respectively processed with a first sliding rod (310) slidably connected to the first sliding hole (303) in the horizontal direction, and a second sliding rod (311) slidably connected to the second sliding hole (312) is respectively arranged at the bottom of each upper unloading plate (301).
7. The culturing device for Tremella fuciformis strain selection and breeding according to claim 1, characterized in that: The bag-supporting mechanism (4) comprises: a lower support plate (16) being rotatably connected to one end of a third connecting rod (403); the other end of the third connecting rod (403) being rotatably connected to a hook (404); a plurality of hooks (404) supporting the opening of a cultivation bag (21); the hook (404) being fixedly connected to a fourth slider (405); a fifth sliding hole (402) being uniformly processed in a circumferential direction of the upper support plate (15); a third slider (401) being slidably connected to the fifth sliding hole (402) being arranged on the fourth slider (405); a second fixing rod (406) being slidably connected to the fourth slider (405) in a horizontal direction being arranged on the third fixing plate (26); a second spring (407) being arranged on the second fixing rod (406); one end of the second spring (407) being fixedly connected to the third fixing plate (26); and the other end of the second spring (407) being fixedly connected to the fourth slider (405).
8. A cultivation method using the cultivation device for Tremella fuciformis strain selection and breeding according to claim 1, characterized in that: The following steps are involved: S1, placing Tremella culture medium into a feed barrel (1): weighing 25-35 parts of Ganoderma lucidum extraction residue, 25-35 parts of lotus seed shells, 10-20 parts of cottonseed shells, 15-25 parts of bran, and 0.5-1.5 parts of gypsum according to mass percentage, stirring evenly to obtain a culture medium with a water content of 55-60%, placing the culture medium into the feed barrel (1), and the culture medium in the feed barrel (1) falls onto two lower discharge plates (316); S2, the culture medium in the feed barrel (1) is automatically bagged in the cultivation bag (21): the lifting mechanism (2) drives the feed barrel (1) on the upper sliding plate (14) to move downward in the vertical direction on the multiple upper support columns (13), and the lifting mechanism (2) drives the culture dish (18) on the lower sliding plate (17) to move upward in the vertical direction on the multiple lower support columns (27). The movement direction of the upper sliding plate (14) is opposite to the movement direction of the lower sliding plate (17). During the upward movement of the culture dish (18) and the cultivation bag (21) driven by the lower sliding plate (17), the bag-holding mechanism (4) holds open the opening of the cultivation bag (21), the outlet end of the feed barrel (1) corresponds to the opening end of the cultivation bag (21), the unloading mechanism (3) on the upper sliding plate (14) opens the opening end of the connecting barrel (322), and the culture medium in the feed barrel (1) enters the cultivation bag (21). After bagging is completed, the cultivation bag (21) is manually tied; S3, sterilization, inoculation, and mycelium cultivation: the cultivation bag (21) containing the culture medium is taken out and placed in a high-pressure steam sterilizer, the temperature is raised to 121° C. and maintained for 1.5 hours, the sterilized culture medium is cooled to below 30° C., and inoculated in a sterile inoculation room, the cultivation bag (21) containing the culture medium is put back into the culture dish (18), the humidity is controlled to 60-70% by spraying water through the water spray pipe (11), and the temperature is controlled to 20-25° C. by the heater (22), and the culture is cultured for 45 days. After the culture medium in the cultivation bag (21) has mycelium and ear buds are formed, the bag mouth of the cultivation bag 21 is opened, the temperature is controlled to 20-23° C., and the relative humidity is maintained at 80-85%. When the ear pieces begin to stretch, the relative humidity is 85-90%; S4, breeding Tremella fuciformis strains: breeding Tremella fuciformis strains by manually observing the Tremella fuciformis in the incubator (6).
9. The culturing method of the tremella fuciformis strain breeding culturing device according to claim 8, characterized in that: In the step S2, the lifting mechanism (2) drives the feed tube (1) on the upper sliding plate (14) to move downward along the vertical direction on the multiple upper support columns (13), and the lifting mechanism (2) drives the culture dish (18) on the lower sliding plate (17) to move upward along the vertical direction on the multiple lower support columns (27) in the following method: the output end of the electric cylinder (205) drives the second connecting plate (204) to move in the vertical direction, the second connecting plate (204) drives the upper sliding plate (14) to move downward along the vertical direction on the multiple upper support columns (13), the second rack (207) on the second connecting plate (204) drives the gear (203) to rotate by meshing with the gear (203), the gear (203) drives the first connecting plate (202) to move in the vertical direction by meshing with the first rack (201) on the first connecting plate (202), and the first connecting plate (202) drives the lower sliding plate (17) to move upward along the vertical direction on the multiple lower support columns (27).
10. The culturing method of the tremella fuciformis strain selection and breeding device according to claim 8, characterized in that: The method for the bag-supporting mechanism (4) to support the opening of the cultivation bag (21) in step S2 is as follows: during the upward movement of the lower sliding plate (17) in the vertical direction, the second contact wheel (20) on the lower sliding plate (17) contacts the second inclined surface of the first contact block (23) at the bottom of the lower support plate (16), the first contact block (23) drives the lower support plate (16) to rotate in the third fixed plate (26), the lower support plate (16) drives the hook (404) to move through the third connecting rod (403), the hook (404) drives the fourth slider (405) to slide in the horizontal direction on the second fixed rod (406), the third slider (401) on the fourth slider (405) slides in the fifth sliding hole (402) of the upper support plate (15), and the hook (404) supports the opening of the cultivation bag (21); The method for the discharge mechanism (3) on the upper sliding plate (14) in step S2 to open the open end of the feed barrel (1) is as follows: during the downward movement of the upper sliding plate (14) in the vertical direction, the first abutment wheels (304) on both sides of the connecting frame (305) respectively abut against the first inclined surface of the second abutment block (24), each connecting frame (305) respectively slides in the horizontal direction in the second fixed plate (309), each connecting frame (305) respectively drives the lower discharge plate (316) to move, each connecting frame (305) respectively drives the first slider (308) to move in the horizontal direction on the first track (307) and the first fixed rod (317) through the first connecting rod (306), the two lower discharge plates (316) are separated, and the second slider (308) on each lower discharge plate (316) is moved. 20) slide in the fourth slide hole (321) of the second fixed plate (309), the third slide bar (315) of each lower unloading plate (316) slides in the third slide hole (314) of the second connecting rod (313) and drives the second connecting rod (313) to rotate, the second slide hole (312) on each second connecting rod (313) slides with the second slide bar (311) at the bottom of the upper unloading plate (301), each second slide bar (311) drives the upper unloading plate (301) to slide in the first fixed plate (302) along the horizontal direction, the first slide bar (310) of each upper unloading plate (301) slides in the first slide hole (303) of the first fixed plate (302), and the two upper unloading plates (301) are combined to block the inner opening end of the feed barrel (1).
Citation Information
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