Artificial moss crust seed source and preparation method and application thereof

Abstract

The invention relates to the technical field of environmental engineering, and particularly discloses an artificial moss crust seed source and a preparation method and application thereof.The preparation method comprises the following steps that soil of a moss crust same-area non-crust land parcel is collected and sterilized to serve as culture medium soil, and the water content of the culture medium soil is regulated and controlled to be 20%-22%; uniformly scattering a wild moss crust seed source on the surface of culture medium soil to complete inoculation, and culturing for 30-40 days under the conditions that the temperature is 15-35 DEG C and the air humidity is 30-100%; and after culture is finished, collecting the artificial moss crust, drying at 20-30 DEG C, and crushing to obtain the artificial moss crust seed source. When the artificial moss crust seed source prepared by the method is used for culturing moss crust in the field, the plant density and the increase rate of coverage are improved.

Description

Technical Field

[0001] The invention relates to the technical field of environmental engineering, and in particular to an artificial moss crust provenance and a preparation method and application thereof. Background Art

[0002] Biological crusts are an important component of healthy ecosystems in arid and semi-arid areas. Moss crusts are an advanced stage of biological crust succession and play an important role in improving soil physical and chemical properties, regulating soil water infiltration and surface runoff, and preventing soil erosion and wind erosion and desertification. Secondly, moss crusts can also affect the growth and health of vascular plants by affecting seed germination and seedling colonization. Therefore, maintaining a certain coverage of moss crusts plays a vital role in the health and sustainable development of ecosystems in arid and semi-arid areas. However, due to human activities and global climate change, the coverage of biological crusts has decreased, and its ecological functions such as carbon fixation, nitrogen fixation, soil and water conservation, and wind and sand fixation have degraded or disappeared, seriously threatening the safety and sustainable development of ecosystems in arid areas. Generally, the natural recovery process of degraded moss crusts is extremely slow and difficult to meet the needs of maintaining ecosystem health. Studies have shown that artificial inoculation and certain cultivation measures can significantly promote the recovery of moss crust coverage and its ecological functions, but the preparation of high-vitality provenance is the key to artificial restoration of moss crusts.

[0003] In the prior art, the soil layer of about 1 cm of wild moss crust is directly collected, and then placed in a natural cool environment to dry, but the different ambient temperatures can cause different physiological reactions in the mosses themselves to adapt to the drying process itself, so that the material accumulated in the mosses is transformed, and then its vegetative propagation ability is affected. In the prior art, the wild moss crust is directly pulverized with a pulverizer after being collected and dried, but the pulverization process temperature is too high, and the size of the stem and leaf fragments can affect the vegetative propagation ability of the provenance. Existing methods are difficult to maintain the vitality of the moss crust provenance, and the vitality of the obtained provenance is unstable, and the post-inoculation culture effect is poor, and even the phenomenon of no moss crust development occurs. Therefore, it is urgent to provide a method for preparing a moss crust provenance with a stable vitality and good post-inoculation culture effect. Summary of the invention

[0004] In order to develop a method for preparing a moss crust provenance with stable vitality and good culture effect after inoculation, the present invention provides an artificial moss crust provenance and its preparation method and application. The artificial moss crust provenance prepared by the present invention increases the growth rate of plant density and coverage when cultivating moss crust in the wild.

[0005] The present invention provides a method for preparing an artificial moss crust provenance, comprising the following steps:

[0006] Soil from a non-crusted plot in the same area as the wild moss crust was collected and sterilized as the culture medium soil, and the water content of the culture medium soil was adjusted to 20% to 22%;

[0007] The wild moss crust seed was inoculated on the surface of the culture medium soil at an inoculation rate of 500 g / m 2 ~700g / m 2 After inoculation, the artificial moss crust is obtained by culturing for 30 to 40 days at a day / night temperature of 20°C to 30°C / 10°C to 15°C and an air humidity of 30% to 100% and then harvesting the cultured moss. The artificial moss crust is obtained by drying the cultured moss at 20°C to 30°C.

[0008] The dried artificial moss crust is crushed into stem and leaf fragments with a length of 1 mm to 2 mm. The temperature is set at 10° C. to 25° C. during the crushing process. After crushing, the artificial moss crust is stirred evenly to obtain the artificial moss crust seed source, which is then stored.

[0009] The process of preparing wild moss crust seed source is as follows: collect wild moss crust, dry it naturally, remove impurities, and then crush it into stem and leaf fragments with a length of 1mm to 2mm as the wild moss crust seed source.

[0010] The artificial moss crust provenance prepared by the present invention is beneficial to maintaining the vitality of the moss crust provenance. On average, each moss plant can sprout 27.53 new branches, which prolongs the storage time. In addition, cultivating moss crust in the wild increases the growth rate of plant density and coverage.

[0011] Furthermore, the temperature during the crushing process after inoculation, cultivation and harvesting was set at 25°C.

[0012] Furthermore, the culture conditions after inoculation are: day / night temperature 20°C to 30°C / 10°C to 15°C, and air humidity 50% to 90%.

[0013] Furthermore, water management is performed after inoculation: the soil moisture content is maintained at 20% to 22%.

[0014] Furthermore, the inoculum amount of wild moss crust is 700g / m 2 .

[0015] Furthermore, the culture medium soil preparation process is as follows: when collecting, dig out the soil from the surface layer of 0 cm to 20 cm, grind and sieve it, and then sterilize it at high temperature to obtain the culture medium soil.

[0016] Furthermore, the storage time is 0 to 120 days.

[0017] The present invention also provides the artificial moss crust provenance prepared by the preparation method of the artificial moss crust provenance.

[0018] The invention also provides application of the artificial moss crust source in cultivating moss crust.

[0019] Furthermore, the artificial moss crust provenance is used to increase the growth rate of plant density and coverage during the process of cultivating moss crust in the wild.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention clarifies the harvesting conditions for maintaining the optimal vegetative reproduction capacity of artificially cultivated moss crusts. The present invention rapidly dries the moss crusts at a temperature of 20°C to 30°C to maintain the optimal vegetative reproduction capacity of the moss crusts, so that the moss crusts have a higher germination rate and the number of new shoots when inoculated in the later stage.

[0022] 2. The present invention provides a method for crushing artificial moss crust seed sources to maintain their vitality. The crushing is carried out at a temperature of 10°C to 25°C. The moss stem and leaf fragments after crushing should be distributed between 1mm and 2mm, so that the seed sources maintain a high vegetative reproduction capacity and are prepared to a size that is conducive to their germination.

[0023] 3. The present invention clarifies the longest storage time for maintaining a certain vegetative reproduction capacity of moss crust provenance. After the artificially cultivated moss crust is prepared as a provenance, the longest storage time should not exceed 120 days to maintain a certain vegetative reproduction capacity of the moss crust provenance, solve the problem of seed preservation when the artificial moss crust provenance is harvested and inoculated at different times, and ensure the reliability of the application of the prepared moss crust provenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 The effects of drying moss crust provenance at different temperatures on germination rate and number of new branches;

[0026] In the figure, A is the effect of 5-30℃ dry moss crust provenance on germination rate;

[0027] B is the effect of 5-30℃ dry moss crust provenance on the number of new branches.

[0028] Figure 2 The effect of different storage time on the germination rate and number of new branches of moss crust provenance at the end of culture.

[0029] In the figure, the effect of storage for 0-200 days on the germination rate of moss crust provenance at the end of culture;

[0030] Effect of storage for 0-200 days on the number of new branches at the end of moss crust provenance culture.

[0031] Figure 3The density and coverage of moss crust plants obtained after the moss crust seed source prepared by the method of the present invention is inoculated and cultured in the field;

[0032] In the figure, A is the density of moss crust strains obtained by inoculating moss crust provenance in the field and culturing for 5-30 days;

[0033] B is the coverage of moss crust obtained by inoculating moss crust sources in the field and culturing them for 5-30 days. DETAILED DESCRIPTION

[0034] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0035] The present invention provides a method for preparing an artificial moss crust provenance, comprising the following steps:

[0036] 1. Collection of wild moss crusts and preparation of wild moss crust provenance

[0037] The moss crust used in the experiment was collected from the Sibu Tree Experimental Field of Shenmu Erosion and Environment National Field Scientific Observation and Research Station in Shenmu City, Shaanxi Province (38°49′39″N, 110°18′38″E). The area has a temperate semi-arid climate with rainy and hot seasons. Rainfall is concentrated in June-September, accounting for about 80.9% of the annual rainfall, and the average annual rainfall is 408.5 mm; the average land surface evaporation is 326.0 mm; the average temperature is 8.9°C, the annual extreme maximum temperature is 38.9°C, the annual extreme minimum temperature is -28.1°C, and the annual average ground temperature is 11.4°C; the average sunshine is 2875.9 hours. The main vegetation in the experimental area includes Artemisia ordosica Krasch., Incarvillea sinensis Lam., Aster altaicus (Willd) Novopokr., Setaria viridis (Linn.) P.Beauv., and Agriophyllum squarrosum (Linn.) Moq.

[0038] When collecting, choose naturally grown and well-developed wild moss crusts, use a small shovel to scoop up a soil layer of about 1 cm, transport it back to the laboratory, and place it in a cool place to dry naturally until constant weight, remove plant residues, soil blocks, stones, etc. that can be seen with the naked eye, and then crush the moss crust into stem and leaf fragments with a length of 1mm to 2mm. After stirring and mixing, obtain the wild moss crust seed source.

[0039] It has been identified that the main moss species of wild moss crusts include Didymodon vinealis, Didymodon tectorum, and Bryum argenteum.

[0040] 2. Artificial propagation of wild moss crust provenance

[0041] Collection of culture medium soil: Collect soil from non-crust plots in the same area as wild moss crust. When collecting, remove surface weeds and dead materials, dig out the surface soil of 0cm to 20cm, transport the soil back to the laboratory and air-dry it naturally, grind it through a 2mm sieve, and sterilize it in a 105℃ oven for 6h, and use it as culture medium soil.

[0042] Inoculation propagation: Use a plastic dish with an inner diameter of 27.5 cm × 18 cm × 7.5 cm as a culture dish, fill each plastic dish with 1 kg of culture medium soil as the culture matrix, smooth the surface of the culture matrix with a small shovel, add distilled water until the water content of the culture matrix is ​​20%, and after the water has completely infiltrated, evenly spread the wild moss crust provenance on the surface of the culture matrix, and inoculate each plastic dish with 35 g of wild moss crust provenance (inoculation amount is 700 g / m 2 ), and cultured in a greenhouse at a temperature of 20°C to 30°C during the day, 10°C to 15°C at night, and 50% to 90% humidity.

[0043] Management: Water management was performed by weighing every two days after inoculation to maintain the soil moisture content at 20%. Weed seedlings growing on the surface were removed with tweezers every 5 days during the cultivation period.

[0044] 3. Harvesting of artificial moss crust

[0045] After one month of cultivation, the plant density and coverage were investigated every 5 days until the density of moss crust plants reached 70 plants / cm -2 When the coverage reaches 60%, it is harvested. When harvesting, use a small shovel to scoop up a soil layer of about 1 cm, remove the excess soil under the crust, until the part where the moss rhizoids and soil particles are bonded to each other is exposed, place the moss crust in a plastic tray, and quickly dry it at 20℃ to 30℃ to constant weight to obtain artificial moss crust for later use.

[0046] 4. Preparation of artificial moss crust seed sources

[0047] The dried artificial moss crust was crushed into stem and leaf fragments with a length of 1 mm to 2 mm using a multifunctional crusher. The temperature was set at 10° C. to 25° C. during the crushing process. After the crushing, the artificial moss crust was stirred evenly to obtain the artificial moss crust source.

[0048] 5. Preservation of artificial moss crust provenance

[0049] The prepared artificial moss crust seed source is put into a sealed bag, and the sealed bag is sealed after removing excess air, and stored in the dark at a low temperature of 5°C to 10°C for 120 days.

[0050] The artificial moss crust provenance prepared by the present invention can maintain the best vegetative reproduction ability of the moss crust provenance, so that it has a higher germination rate and the number of sprouted new branches during later inoculation, and after being inoculated and cultivated in the field, a good moss crust can be formed.

[0051] Example 1: A method for preparing an artificial moss crust provenance.

[0052] 1. Experimental Methods

[0053] 1. Collection of wild moss crusts and preparation of wild moss crust provenance

[0054] The moss crusts used in the experiment were collected at the Sibu Tree Experimental Field of the National Field Scientific Observation and Research Station for Erosion and Environment in Shenmu, Shaanxi Province (38°49′39″N, 110°18′38″E). When collecting, naturally grown and well-developed moss crusts were selected, and a 1 cm soil layer was scooped with a small shovel. After being transported back to the laboratory, they were placed in a cool place to dry naturally to constant weight, and plant residues, soil blocks, stones, etc. that were visible to the naked eye were removed. The dried moss crusts were then crushed into stem and leaf fragments with a length of 1 mm to 2 mm using a multi-functional crusher (BJ-200), and the wild moss crust provenance was obtained after stirring and mixing.

[0055] 2. Artificial propagation of wild moss crust provenance

[0056] Collection of culture medium soil: Collect soil from non-crust plots in the same area as wild moss crusts. When collecting, remove surface weeds and dead materials, dig 20 cm of soil from the surface, transport the soil back to the laboratory and air-dry it naturally. After grinding through a 2 mm sieve, sterilize it in an oven at 105°C for 6 hours and use it as culture medium soil.

[0057] Inoculation propagation: Use a plastic dish with an inner diameter of 27.5 cm × 18 cm × 7.5 cm as a culture dish. Sterilize, wash and dry the culture dish. Fill each plastic dish with 1 kg of culture soil as the culture matrix. Smooth the surface of the culture matrix with a small shovel. Add 200 mL of distilled water to make the water content of the culture soil 20%. After the water has completely infiltrated, evenly spread the wild moss crust provenance on the surface of the culture matrix. Inoculate each plastic dish with 35 g of wild moss crust provenance (inoculation amount is 700 g / m 2 ), cultured in a greenhouse with two layers of shade nets for shading, the culture temperature was set at 20℃ / 12℃ during the day / night, and the air humidity was 70%.

[0058] Management: After inoculation, water management was performed by weighing every two days to maintain the soil moisture content at 20%. Weed seedlings growing on the surface were removed with tweezers every 5 days during the cultivation period. The total cultivation period was 35 days.

[0059] 3. Harvesting of artificial moss crust

[0060] After one month of cultivation, the plant density and coverage were investigated every 5 days. When the density of moss crust plants reached 70 plants cm -2 When the coverage reaches 60%, the culture is stopped and the moss crust is harvested. When harvesting, a small shovel is used to scoop up a soil layer of about 1 cm, and the excess soil under the crust is removed until the part where the moss rhizoids and soil particles are bonded to each other is exposed. The crust is placed in a plastic tray, placed in an incubator, and the drying temperature is set to 30°C. Dry to constant weight to obtain an artificial moss crust.

[0061] Plant density: Place five 2-cm square plots at the four corners and in the middle of each culture dish, count the number of moss plants in each plot, and calculate the moss plant density in units of plants / cm -2 .

[0062] Coverage: A self-made square plot with a side length of 5 cm was evenly divided into 25 small squares with a side length of 1 cm. The plot was randomly placed in a culture dish 5 times, and the number of moss plants at a fixed point in the small square was counted. The percentage of the total number of points was taken as the moss crust coverage, and the average value was taken in %.

[0063] 4. Preparation of artificial moss crust seed sources

[0064] The dried artificial moss crust was crushed into stem and leaf fragments with a length of 1 mm to 2 mm using a multifunctional crusher. The temperature was set to 25°C during the crushing process. After crushing, the artificial moss crust was stirred evenly to obtain the artificial moss crust seed source, which was stored at 6°C for 120 days.

[0065] Example 2: A method for preparing an artificial moss crust provenance.

[0066] The specific steps are the same as those in Example 1, except that:

[0067] The drying temperature during the harvesting process of the artificial moss crust was set at 25°C.

[0068] Example 3: A method for preparing an artificial moss crust provenance.

[0069] The specific steps are the same as those in Example 1, except that:

[0070] The drying temperature during the harvesting process of the artificial moss crust was set at 20°C.

[0071] Example 4: A method for preparing an artificial moss crust provenance.

[0072] The specific steps are the same as those in Example 1, except that:

[0073] The artificial moss crust seed source was prepared and stored for 40 days.

[0074] Example 5: A method for preparing an artificial moss crust provenance.

[0075] The specific steps are the same as those in Example 1, except that:

[0076] The artificial moss crust seed source was prepared and stored for 80 days.

[0077] Comparative Example 1: A method for preparing an artificial moss crust seed source.

[0078] The specific steps are the same as those in Example 1, except that:

[0079] The drying temperature during the harvesting process of the artificial moss crust was set at 5°C.

[0080] Comparative Example 2: A method for preparing an artificial moss crust seed source.

[0081] The specific steps are the same as those in Example 1, except that:

[0082] The drying temperature during the harvesting process of the artificial moss crust was set at 10°C.

[0083] Comparative Example 3: A method for preparing an artificial moss crust seed source.

[0084] The specific steps are the same as those in Example 1, except that:

[0085] The drying temperature during the harvesting process of the artificial moss crust was set at 15°C.

[0086] Comparative Example 4: A method for preparing an artificial moss crust seed source.

[0087] The specific steps are the same as those in Example 1, except that:

[0088] The artificial moss crust seed source was prepared and stored for 160 days.

[0089] Comparative Example 5: A method for preparing an artificial moss crust seed source.

[0090] The specific steps are the same as those in Example 1, except that:

[0091] The artificial moss crust seed source was prepared and stored for 200 days.

[0092] 1. The artificial moss crust provenances prepared in the above Examples 1-3 and Comparative Examples 1-3 were inoculated and cultured to study the effects of artificial moss crust provenances at different drying temperatures and different storage times on the vegetative propagation capacity.

[0093] a. Experimental design:

[0094] (1) Experimental design of the effect of different drying temperatures on vegetative propagation capacity

[0095] The artificial moss crust provenance prepared in Example 1-Example 3 and Comparative Example 1-Comparative Example 3 was used as the provenance for inoculation and culture: a six-well plate with a cover (each hole has a diameter of 35 mm and a depth of 12 mm) was disinfected, cleaned and dried, 12 g of soil matrix was filled in each well, and 2.4 mL of distilled water was added to make the soil mass moisture content of the culture medium 20%. Then, 2 mm of the stem tip of the moss plant in the artificial moss crust provenance was selected and inoculated in the six-well plate, 5 plants were inoculated in each well, 5 six-well plates were used for each treatment as a repeat, and a total of 150 moss inocula were covered with a transparent cover after inoculation.

[0096] (2) Experimental design on the effect of different storage times on vegetative propagation capacity

[0097] The artificial moss crust provenance prepared in Example 1, Example 4-Example 5 and Comparative Example 4-Comparative Example 5 were used as provenance for inoculation and culture: a six-well plate with a cover (each hole has a diameter of 35 mm and a depth of 12 mm) was disinfected, cleaned and dried, 12 g of soil matrix was filled in each hole, and 2.4 mL of distilled water was added to make the soil mass moisture content of the culture medium 20%. Then, 2 mm of the stem tip of the moss plant in the artificial moss crust provenance was selected and inoculated in the six-well plate, 5 plants were inoculated in each hole, and 5 six-well plates were used for each treatment as a repeat, with a total of 150 moss inocula, and a transparent cover was covered after inoculation.

[0098] b. Cultivation process

[0099] The six-well plate inoculated with (1)-(2) above was placed in an incubator for cultivation. The incubator was set with a photoperiod of 14h / 10h, a light intensity of 4400Lux, a temperature of 22℃ / 12℃, and a humidity of 80%. Water management was performed by weighing method every 5 days to maintain the soil moisture content at 20%. The germination rate and the number of new branches were observed and recorded every 5 days. The experiment was terminated when the number of new branches tended to be stable. The cultivation time was 45 days.

[0100] c. Observation indicators and methods

[0101] Germination rate: observe the number of moss plants that sprout new branches, divide it by the total number of moss plants in each treatment and take the average value as the germination rate, in %.

[0102] Number of new branches: Count the number of new branches sprouted by each moss strain and take the average value, unit: piece.

[0103] 2. Experimental Results

[0104] 1. Effects of drying moss crust provenance at different temperatures on germination rate and number of new branches

[0105] Figure 1 The germination rate and number of new branches of dry moss crust provenance at different temperatures. Figure 1 It can be seen that the artificially cultivated moss crusts were rapidly dried in the temperature range of 5℃~30℃, and the germination rate of mosses generally showed an upward trend as the temperature rose. The germination rate of rapid drying at 30℃ was the highest, reaching 97.96%, followed by rapid drying at 20℃. The rapid drying germination rates at 20℃ and 30℃ were significantly higher than those at 5℃ and 15℃. The trend of the number of new branches with different drying temperatures was similar to that of rapid drying of germination rate. Within the experimental temperature range, the number of new branches showed an upward trend as the temperature increased. Rapid drying at 30℃ had the largest number of new branches, with an average of 27.53 new branches per moss, which was significantly higher than that at 5℃~15℃. The above test results show that rapid drying at 30℃ is beneficial to maintaining the vitality of the moss crust provenance.

[0106] 2. Effects of different storage times on the germination rate and number of new branches at the end of moss crust provenance culture

[0107] Figure 2 The germination rate and number of new branches at the end of the culture of moss crust provenance after different storage times. Figure 2 It can be seen that the germination rate of mosses showed a downward trend with the extension of storage time. When the storage time was 0 to 40 days, the germination rate of mosses was higher than 95%. When the storage time was 120 days, the germination rate of mosses decreased to 90.67%. When the storage time was further extended, the germination rate was lower than 90%. When the storage time reached 160 to 200 days, the germination rates of mosses were 85.35% and 85.33%, respectively, which was about 10% lower than before storage, significantly lower than before storage. During the experimental storage time, the number of new branches and the germination rate had similar trends. Before storage, the number of new branches of mosses was the largest, which was 27.53. There was no significant difference in the number of new branches within the storage time of 0 to 120 days. When the storage time reached 160 days, the number of new branches decreased to 22.03, which was significantly lower than before storage. The above test results show that when the storage time reaches more than 120 days, the nutritional reproduction capacity of moss crust provenance will be significantly reduced.

[0108] Application Example 1: Application of the artificial moss crust provenance prepared in Example 1 in the cultivation of moss crust in the wild.

[0109] 1. Experimental Materials and Methods

[0110] 1. Test materials

[0111] In Example 1, the moss crust provenance was artificially cultivated.

[0112] Sample site selection: The experiment took the Sibu Tree Experimental Field of the National Field Scientific Observation and Research Station for Erosion and Environment in Shenmu, Shaanxi as the research area. In the experimental area, flat and undisturbed sample sites were selected, and square plots with a side length of 1m were built, and the four corners of the plots were marked with bamboo poles.

[0113] 2. Test steps

[0114] 1) Inoculation of artificial moss crust seed sources

[0115] Before inoculation, the physical crust on the ground was broken, and 2 mm of water was poured. After the water completely infiltrated, 700 g of the artificial moss crust seed source prepared in Example 1 was evenly spread on the soil surface in each plot, and then 3 mm of water was poured. A total of 4 plots were set as replicates.

[0116] 2) Moss culture process

[0117] Water management was performed at 8:00 am every day, with 3 mm of water applied to keep the soil moist, and watering was stopped on rainy days. During the cultivation period, two layers of 1.2 m × 1.2 m shade nets were used for shading, and plant density and coverage were observed and recorded every 5 days.

[0118] 3) Observation indicators and methods

[0119] Plant density: Place five 5-cm square plots at the four corners and in the middle of each plot, count the number of moss plants in each plot, and calculate the moss plant density in units of plants / cm -2 .

[0120] Coverage: A self-made square plot with a side length of 25 cm was evenly divided into 25 small squares with a side length of 5 cm. The plot was randomly placed in the community 5 times, and the number of moss plants at a fixed point in the small square was counted. The percentage of the total number of points was the moss crust coverage, and the average value was taken in %.

[0121] 2. Results and Analysis

[0122] Figure 3 The density and coverage of the artificial moss crusts prepared in Example 1 are shown in Figure 1. Figure 3 It can be seen that the strain density increased rapidly during the culture period. On the fifth day of inoculation, the strain density was only 1.01 strains cm -2On the 30th day of cultivation, the plant density increased rapidly to 23.84 plants cm -2 , which is 23.60 times that of the 5th day of inoculation. The coverage of moss crusts increased rapidly in the first 20 days of inoculation, reaching 26.67%, and gradually stabilized after 20 days. The coverage was 30.33% on the 30th day of inoculation, which was 3.37 times that of the 5th day of inoculation. The test results show that the artificial moss crusts prepared by the method of the present invention can form good moss crusts after field inoculation and auxiliary cultivation.

[0123] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0124] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing an artificial moss crust provenance, characterized in that: The steps include: Soil from a non-crusted plot in the same area as the wild moss crust was collected and sterilized as the culture medium soil, and the water content of the culture medium soil was adjusted to 20% to 22%; The wild moss crust seed was inoculated on the surface of the culture medium soil at an inoculation rate of 500 g / m 2 -700 g / m 2 After inoculation, the artificial moss crust is obtained by culturing for 30 to 40 days at a day / night temperature of 20°C to 30°C / 10°C to 15°C and an air humidity of 30% to 100% and then harvesting the cultured moss. The artificial moss crust is obtained by drying the cultured moss at 20°C to 30°C. The dried artificial moss crust is crushed into stem and leaf fragments with a length of 1 mm to 2 mm. The temperature is set at 10° C. to 25° C. during the crushing process. After crushing, the artificial moss crust is stirred evenly to obtain the artificial moss crust seed source, which is then stored.

2. The method for preparing the artificial moss crust provenance according to claim 1, wherein The temperature was set at 25°C during the crushing process after inoculation and harvesting.

3. The preparation method of artificial moss crust provenance according to claim 1, characterized in that, The culture conditions after inoculation are: day / night temperature 20°C ~ 30°C / 10°C ~ 15°C, air humidity 50% ~ 90%.

4. The preparation method of artificial moss crust provenance according to claim 1, characterized in that, Carry out water management after inoculation: maintain soil moisture content at 20% to 22%.

5. The preparation method of artificial moss crust provenance according to claim 1, characterized in that, The process of preparing the culture medium soil is as follows: when collecting, dig the soil from the surface layer of 0 cm to 20 cm, grind and sieve it, and then sterilize it at high temperature to obtain the culture medium soil.

6. The preparation method of artificial moss crust provenance according to claim 1, characterized in that, The inoculation amount of wild moss crust is 700g / m 2 .

7. The preparation method of artificial moss crust provenance according to claim 1, characterized in that, The storage time is 0 to 120 days.

8. An artificial moss crust provenance obtained by the method for preparing an artificial moss crust provenance according to any one of claims 1 to 7.

9. Use of the artificial moss crust provenance according to claim 8 in cultivating moss crust.

10. Use of the artificial moss crust provenance according to claim 9 in cultivating moss crusts, characterized in that: The artificial moss crust seed source is used to increase the growth rate of plant density and coverage during the process of cultivating moss crust in the wild.

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