Method for promoting growth of root system of anoectochilus formosanus

Abstract

The invention provides a method for promoting growth of an anoectochilus formosanus root system. According to the method for promoting the growth of the anoectochilus formosanus root system, the anoectochilus formosanus root system growth promoting solution is adopted to carry out root irrigation operation on the anoectochilus formosanus plant, so that the anoectochilus formosanus root system growth promoting solution infiltrates the root of the anoectochilus formosanus Wherein the anoectochilus formosanus root system growth promoting solution is an endophytic fungus culture solution of orange yellow phoenix flower. According to the method for promoting the growth of the anoectochilus formosanus root system, the growth of the anoectochilus formosanus root system can be effectively promoted, so that the rapid growth of an anoectochilus formosanus plant is promoted.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, in particular to a method for promoting the growth of the root system of roxburghii. Background Art

[0002] The whole plant of Anoectochilus roxburghii can be used as medicine. It has a mild and sweet taste and can clear away heat and cool blood, dispel wind and dampness, detoxify, relieve pain, and relieve cough. It is mainly used to treat difficult diseases such as hemoptysis, bronchitis, nephritis, cystitis, diabetes, hematuria, rheumatoid arthritis, and tumors. As a result, the demand in the domestic and foreign markets continues to rise, leading to a sharp reduction in wild resources. In order to ease the tense situation of wild Anoectochilus roxburghii resources, people have also begun to pay attention to and study the ecological habits, growth conditions, and artificial breeding techniques of Anoectochilus roxburghii, in order to protect and breed Anoectochilus roxburghii through scientific methods.

[0003] At present, the artificial cultivation of golden thread vine has achieved initial success. The promotion of this method can not only protect wild golden thread vine from the threat of over-exploitation, but also provide people with a sustainable and stable source of golden thread vine. For example, the Chinese invention patent application with application number 201210442593.8 and the Chinese invention patent application with application number 201510488134.7 both use tissue culture to achieve rapid seedling cultivation of golden thread vine. However, due to the underdeveloped root system of golden thread vine, the golden thread vine plants grow slowly, and it is still difficult to meet market demand. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a method for promoting the root growth of roxburghii roxburghii, which can effectively promote the root growth of roxburghii roxburghii and thus promote the rapid growth of roxburghii roxburghii plants.

[0005] The objective of the present invention is achieved through the following technical solutions:

[0006] A method for promoting root growth of roxburghii roxburghii, comprising: performing root irrigation operation on roxburghii roxburghii plants using roxburghii roxburghii root growth-promoting liquid, so that the roxburghii roxburghii root growth-promoting liquid infiltrates the roots of the roxburghii roxburghii plants;

[0007] Wherein, the anoectochilus root growth promoting liquid is the endophytic fungus culture liquid of the orange-yellow jade flower.

[0008] In one embodiment, the root irrigation operation of the roxburghii roxburghii plant is performed using the roxburghii roxburghii root growth promoting liquid, comprising the following steps:

[0009] Obtaining the roxburghii roxburghii plant;

[0010] Performing a cultivation treatment on the roxburghii roxburghii plant so that the roxburghii roxburghii plant is planted in a cultivation medium;

[0011] Punching the cultivation substrate to form a plurality of holes on the cultivation substrate, each of the holes being arranged adjacent to the root system of the roxburghii plant;

[0012] The borehole is irrigated with the roxburghii root growth-promoting liquid so that the roxburghii root growth-promoting liquid can flow back into the borehole.

[0013] In one embodiment, after the step of irrigating the borehole with the roxburghii root growth-promoting liquid, the method for promoting the root growth of roxburghii further comprises the following steps:

[0014] The drill hole is filled and leveled so that the cultivation substrate can level the drill hole.

[0015] In one embodiment, the cultivation medium is peat soil.

[0016] In one embodiment, the steps of obtaining the golden thread lily root growth promoting liquid are as follows:

[0017] Pick wild orange-yellow hibiscus flowers;

[0018] Performing rhizome treatment on the orange-yellow jade flower to clean the surface of the rhizome of the orange-yellow jade flower and obtain new nutrient roots that form mycorrhizae;

[0019] Scraping the bacterial mass from the newly-born nutrient root to absorb the bacterial mass on the newly-born nutrient root to obtain the orange-yellow jade flower bacterial community;

[0020] The acanthocephalum fungus community is subjected to a proliferation and cultivation operation to obtain the roxburghii root system growth-promoting liquid.

[0021] In one embodiment, the rhizome treatment of the orange-yellow hibiscus flower specifically comprises the following steps:

[0022] The rhizome of the orange-yellow hibiscus flower is washed and cut to remove particles on the surface of the rhizome of the orange-yellow hibiscus flower, thereby obtaining the rhizome segments of the orange-yellow hibiscus flower;

[0023] Deeply cleaning the rhizome segments of the orange-yellow jasmine to remove bacteria and harmful substances on the surface of the rhizome segments of the orange-yellow jasmine;

[0024] The orange-yellow Jade Flower rhizome segment after deep cleaning is cleaned to remove liquid on the surface of the orange-yellow Jade Flower rhizome segment to obtain new nutrient roots.

[0025] In one embodiment, the rhizome of the orange-yellow jasmine flower is washed and cut, and the specific operation is as follows:

[0026] The rhizome of the orange-yellow jasmine flower is rinsed under running water, and then the rhizome of the orange-yellow jasmine flower is cut into segments to obtain the rhizome segments of the orange-yellow jasmine flower.

[0027] In one embodiment, the length of the rhizome segment of the orange-yellow jasmine flower is 1 cm to 2 cm.

[0028] In one embodiment, the rhizome segment of the orange-yellow jasmine flower is subjected to a deep cleaning treatment, and the specific operation is as follows:

[0029] The rhizome segments of the orange-yellow jasmine flower are immersed in a sodium hypochlorite solution, and then taken out and immersed in an ethanol solution.

[0030] In one embodiment, the orange-yellow jasmine rhizome segment after deep cleaning is cleaned, and the specific operation is as follows:

[0031] The rhizome segment of the orange-yellow jasmine flower is rinsed with sterile water for at least three times, and then the liquid on the surface of the rhizome segment of the orange-yellow jasmine flower is absorbed with sterile absorbent paper.

[0032] Compared with the prior art, the present invention has at least the following advantages:

[0033] The method for promoting root growth of golden thread vine of the present invention comprises the following steps: using the endophytic fungus culture solution of orange-yellow jade flower as the golden thread vine root growth-promoting solution, and irrigating the root of the golden thread vine plant with the golden thread vine root growth-promoting solution, so that the root of the golden thread vine is infiltrated by the golden thread vine root growth-promoting solution, that is, the golden thread vine root growth-promoting solution directly acts on the root, promoting the endophytic fungus of orange-yellow jade flower and the root of the golden thread vine to coexist, thereby effectively promoting the root growth of the golden thread vine, namely, increasing the number of the root of the golden thread vine, and enhancing the growth intensity of the root of the golden thread vine, thereby facilitating the intake of nutrients of the golden thread vine plant, and better realizing the rapid growth of the golden thread vine plant. DETAILED DESCRIPTION

[0034] In order to facilitate understanding of the present invention, the present invention will be described more fully below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly understood.

[0035] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0037] The present application provides a method for promoting the root growth of Anoectochilus roxburghii. In order to better understand the method for promoting the root growth of Anoectochilus roxburghii of the present application, the method for promoting the root growth of Anoectochilus roxburghii of the present application is further explained below:

[0038] A method for promoting root growth of Anoectochilus roxburghii in one embodiment comprises performing root irrigation operation on Anoectochilus roxburghii plants using Anoectochilus roxburghii root growth-promoting liquid, so that the Anoectochilus roxburghii root growth-promoting liquid infiltrates the roots of the Anoectochilus roxburghii plants; wherein the Anoectochilus roxburghii root growth-promoting liquid is a culture liquid of endophytic fungi of the orange-yellow Jade Ornament.

[0039] The above-mentioned method for promoting the root growth of golden thread vine uses the endophytic fungus culture solution of orange-yellow jade flower as the golden thread vine root growth-promoting solution, and irrigates the roots of the golden thread vine plant with the golden thread vine root growth-promoting solution, so that the roots of the golden thread vine are infiltrated by the golden thread vine root growth-promoting solution, that is, the golden thread vine root growth-promoting solution directly acts on the roots, promotes the symbiosis of the endophytic fungi of the orange-yellow jade flower and the roots of the golden thread vine, and then effectively promotes the root growth of the golden thread vine, that is, increases the number of roots of the golden thread vine, and enhances the growth intensity of the roots of the golden thread vine, which is beneficial to the intake of nutrients by the golden thread vine plant, and better realizes the rapid growth of the golden thread vine plant.

[0040] In one embodiment, the orange-yellow jade flower is collected from Xiangtoushan Nature Reserve. Further, the orange-yellow jade flower is collected from a location at 114° east longitude, 23° north latitude, and 460m above sea level.

[0041] In one embodiment, the root irrigation operation of the roxburghii roxburghii plant is performed using the roxburghii roxburghii root growth promoting liquid, comprising the following steps:

[0042] S110, obtaining a roxburghii roxburghii plant. Further, the roxburghii roxburghii plant is a tissue culture seedling of a wild roxburghii roxburghii native to Longmen and provided by Longmen County Hujinyuan Ecological Agriculture Development Co., Ltd.

[0043] S120, performing a cultivation process on the roxburghii roxburghii plants so that the roxburghii roxburghii plants are planted in a cultivation medium.

[0044] S130, drilling the cultivation substrate to form a plurality of holes on the cultivation substrate, each hole being disposed adjacent to a root system of the roxburghii plant.

[0045] S140, the borehole is irrigated with a growth-promoting liquid for the root system of the golden thread vine, so that the growth-promoting liquid for the root system of the golden thread vine can flow back into the borehole.

[0046] It can be understood that the obtained golden thread vine plants are planted in the cultivation matrix, and the golden thread vine root growth-promoting liquid is poured into the drilled holes in cooperation with the drilling of the cultivation matrix, so that the golden thread vine root growth-promoting liquid can effectively contact and infiltrate the root system of the golden thread vine plant, thereby facilitating the endophytic fungi of the orange-yellow jade flower and the root system of the golden thread vine plant to form a symbiotic state. It can also be understood that if the golden thread vine plant is sprayed or watered directly, it is easy to cause the golden thread vine root growth-promoting liquid to exist only in the soil, and the soil is not suitable for the survival and growth of all fungi, and then the golden thread vine root growth-promoting liquid will be lost more in the soil, affecting the promotion of the golden thread vine root growth. If the amount of golden thread vine root growth-promoting liquid used is increased, the content of endophytic fungi of the orange-yellow jade flower in the soil will be greatly increased, which will easily cause the pathogenic bacteria of golden thread vine to have an advantage, but affect the growth of golden thread vine plants.

[0047] In one embodiment, after the step of filling the drill hole with the golden thread vine root growth promoting liquid, the method for promoting the root growth of the golden thread vine further includes the following step: filling the drill hole so that the cultivation matrix fills the drill hole, further reducing the loss of the golden thread vine root growth promoting liquid.

[0048] In one embodiment, the cultivation medium is peat soil, which is conducive to the growth of the golden thread vine plant.

[0049] In one embodiment, the amount of the root growth-promoting liquid of the golden thread vine is 5ml to 15ml. It can be understood that when the root system of the golden thread vine plant is fully infiltrated with the root system growth-promoting liquid of the golden thread vine, the effect of promoting the growth of the root system of the golden thread vine plant is better when the amount of the root growth-promoting liquid of the golden thread vine is 5ml to 15ml. If the amount used is small, the advantage of the endophytic fungi of the orange-yellow jade flower is poor, and it is difficult to form a symbiotic state with the root system of the golden thread vine, and it is difficult to promote the growth of the root system of the golden thread vine plant; if the amount used is large, the advantage of the endophytic fungi of the orange-yellow jade flower is strong, which will affect the advantage of the native fungi of the root system of the golden thread vine plant, and then affect the growth of the root system of the golden thread vine plant.

[0050] In one embodiment, the steps of obtaining the golden thread vine root growth promoting liquid are as follows:

[0051] S210, picking wild orange-yellow hibiscus flowers;

[0052] S220, treating the rhizomes of the orange-yellow jade flower to clean the surface of the rhizomes of the orange-yellow jade flower to obtain new nutrient roots that form mycorrhizae;

[0053] S230, performing a bacterial mass scraping operation on the newly-born nutrient root to absorb the bacterial mass on the newly-born nutrient root to obtain the orange-yellow jade flower bacterial population;

[0054] S240, performing proliferation and culture operations on the bacteria community of the orange-yellow jade flower to obtain a growth-promoting liquid for the root system of the golden thread vine.

[0055] It can be understood that the endophytic fungi of the orange-yellow jade flower are cultured together to form a root growth-promoting liquid for golden thread vine, among which the presence of at least one endophytic fungus of the orange-yellow jade flower can effectively promote the growth of the root system of the golden thread vine plant. The present application does not purify and separate the specific types of endophytic fungi, but only protects the endophytic fungi of the orange-yellow jade flower to form a root growth-promoting liquid for golden thread vine after proliferation and culture, which has a good effect of promoting the growth of the roots of the golden thread vine plant when used. It is worth mentioning that although there are multiple endophytic fungi in the root growth-promoting liquid for golden thread vine, it will not affect the growth of the root system of the golden thread vine plant when the usage is controlled.

[0056] In one embodiment, the rhizome of the orange-yellow hibiscus is processed, which specifically comprises the following steps:

[0057] S310, washing and cutting the rhizome of the orange-yellow jasmine to remove particles on the surface of the rhizome of the orange-yellow jasmine to obtain rhizome segments of the orange-yellow jasmine. Further, there are a plurality of rhizome segments of the orange-yellow jasmine.

[0058] S320, performing deep cleaning on the rhizome segments of the orange-yellow jade flower to remove bacteria and harmful substances on the surface of the rhizome segments of the orange-yellow jade flower;

[0059] S330, cleaning the rhizome segments of the orange-yellow Jade Flower that have been deeply cleaned to remove liquid on the surface of the rhizome segments of the orange-yellow Jade Flower to obtain new nutrient roots.

[0060] It can be understood that by cleaning the particles, bacteria and harmful substances on the surface of the rhizome of the orange-yellow jade flower separately, it is easier to obtain the endophytic fungal flora of the orange-yellow jade flower with higher purity at the newly formed nutrient roots, which is beneficial to reduce the influence of miscellaneous bacteria or other substances on the growth of the flora at the newly formed nutrient roots during the proliferation culture process.

[0061] In one of the embodiments, the rhizomes of the orange-yellow jade flower are rinsed and cut, and the specific operation is: the rhizomes of the orange-yellow jade flower are rinsed under tap water, and then the rhizomes of the orange-yellow jade flower are cut into segments to obtain orange-yellow jade flower rhizome segments, which better ensures the effective removal of particulate matter on the surface of the rhizomes of the orange-yellow jade flower, and the formation of the rhizome segments of the orange-yellow jade flower is conducive to the scraping of the bacterial mass of the newly formed nutrient roots in the later stage.

[0062] In one embodiment, the length of the rhizome segment of the orange-yellow Jade Phoenix flower is 1 cm to 2 cm, which is conducive to scraping the bacterial mass of the newly formed nutrient roots in the later stage.

[0063] In one of the embodiments, the rhizome segments of the orange-yellow jasmine flower are subjected to a deep cleaning treatment, and the specific operation is: the rhizome segments of the orange-yellow jasmine flower are soaked in a sodium hypochlorite solution, and then taken out and soaked in an ethanol solution, thereby effectively removing bacteria and harmful substances on the surface of the rhizome segments of the orange-yellow jasmine flower.

[0064] In one embodiment, the mass percentage of the sodium hypochlorite solution is 2.5%. Further, the rhizome segments of the orange-yellow jasmine flower are immersed in the sodium hypochlorite solution for 0.5 to 1 minute, which further effectively removes bacteria and harmful substances on the surface of the rhizome segments of the orange-yellow jasmine flower.

[0065] In one embodiment, the mass percentage of the ethanol solution is 70%. Further, the rhizome segments of the orange-yellow jasmine flower are immersed in the ethanol solution for 0.8 min to 1 min, which further effectively removes bacteria and harmful substances on the surface of the rhizome segments of the orange-yellow jasmine flower.

[0066] In one of the embodiments, the rhizome segments of the orange-yellow jade flower after deep cleaning are cleaned, and the specific operation is: the rhizome segments of the orange-yellow jade flower are rinsed with sterile water at least three times, and then the liquid on the surface of the rhizome segments of the orange-yellow jade flower is absorbed with sterile absorbent paper, thereby further reducing the residues of various reagents or miscellaneous bacteria on the surface of the orange-yellow jade flower.

[0067] In one of the embodiments, a mycelium scraping operation is performed on the newly-born nutrient roots. Specifically, the newly-born nutrient roots are placed in a culture dish containing sterile water, and a sterilized inoculation needle is used to scrape a single mycelium mass of the newly-born nutrient roots, thereby effectively obtaining the endophytic fungi on the newly-born nutrient roots.

[0068] In one embodiment, before the step of performing proliferation and cultivation operations on the orange-yellow jade flower bacterial community and after the step of performing bacterial mass scraping operations on the newly formed nutrient roots, the golden thread vine root growth promoting liquid also includes the following acquisition step: selectively culturing the orange-yellow jade flower bacterial community to inhibit the excessive growth of miscellaneous bacteria in the orange-yellow jade flower bacterial community.

[0069] In one of the embodiments, the flora of the orange-yellow jade flower is selectively cultured, and the specific operation is: caspofungin is added to the PDB medium. Further, the amount of caspofungin used is 0.05g / L to 0.3g / L. It can be understood that since there are many types of endophytic fungi in the orange-yellow jade flower, and at least one of the endophytic fungi of the orange-yellow jade flower has a promoting effect on the growth of the root system of the golden thread lily, therefore, considering that in order to better achieve the effective growth of the endophytic fungi of the orange-yellow jade flower that has a promoting effect on the growth of the root system of the golden thread lily, caspofungin is used to selectively proliferate and culture the endophytic fungi of the orange-yellow jade flower, so that the golden thread lily root system growth-promoting liquid obtained has a better promoting effect on the growth of the root system of the golden thread lily plant.

[0070] In one embodiment, the preparation of PDB culture medium is specifically performed as follows: 200 g of peeled potatoes are boiled in boiling water for 30 min, filtered through gauze, 20 g of glucose is added, water is added to make the volume to 1 L, and sterilized at 121° C. for 30 min.

[0071] In one of the embodiments, the orange-yellow jade flower fungus community is subjected to a proliferation and cultivation operation, and the specific operation is: the orange-yellow jade flower fungus community is inoculated into a PDB medium, and then the PDB medium is placed in a shaker at a temperature of 25°C to 28°C for cultivation. Further, the rotation speed of the shaker is 110r / min to 120r / min. Further, the specification of the PDB medium is 100ml to 150ml. Further, the PDB medium is placed in a 25°C to 28°C shaker for at least 3 days, which effectively realizes the rapid proliferation of the orange-yellow jade flower fungus community, reduces the mutual interference of the orange-yellow jade flower fungus community, and thus ensures that the golden thread vine root growth-promoting liquid formed after the proliferation and cultivation of the orange-yellow jade flower fungus community has a promoting effect on the growth of the root system of the golden thread vine plant. It can be understood that if the orange-yellow jade flower flora grows excessively, it will affect the mutual interference of the orange-yellow jade flower flora, and affect the effect of the formed golden thread vine root growth-promoting liquid on the growth of the roots of the golden thread vine plants; if the orange-yellow jade flower flora does not grow fully, the concentration of endophytic fungi of the orange-yellow jade flower in the golden thread vine root growth-promoting liquid will be low, which will make it difficult to meet the needs of promoting the root growth of the golden thread vine plants.

[0072] In one embodiment, the PDB culture medium is placed in a shaker at a temperature of 25°C to 28°C for culture, and then the PDB culture medium is broken and mixed so that the clumping hyphae in the PDB culture medium are evenly dispersed in the PDB culture medium to obtain a golden thread vine root growth promoting liquid.

[0073] Compared with the prior art, the present invention has at least the following advantages:

[0074] The method for promoting root growth of golden thread vine of the present invention comprises the following steps: using the endophytic fungus culture solution of orange-yellow jade flower as the golden thread vine root growth-promoting solution, and irrigating the root of the golden thread vine plant with the golden thread vine root growth-promoting solution, so that the root of the golden thread vine is infiltrated by the golden thread vine root growth-promoting solution, that is, the golden thread vine root growth-promoting solution directly acts on the root, promoting the endophytic fungus of orange-yellow jade flower and the root of the golden thread vine to coexist, thereby effectively promoting the root growth of the golden thread vine, namely, increasing the number of the root of the golden thread vine, and enhancing the growth intensity of the root of the golden thread vine, thereby facilitating the intake of nutrients of the golden thread vine plant, and better realizing the rapid growth of the golden thread vine plant.

[0075] Some specific examples are listed below, and if % is mentioned, it means percentage by weight. It should be noted that the following examples do not exhaust all possible situations, and the materials used in the following examples can be obtained from commercial sources unless otherwise specified.

[0076] Example 1

[0077] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0078] Preparation of PDB medium: Boil 200g of potatoes (peeled) in boiling water for 30min, filter with gauze, add 20g of glucose, add water to make up to 1L, put into electric pressure steam sterilizer at 121℃ for 30min, divide into conical flasks and set aside for later use. The capacity of the conical flask is 100ml.

[0079] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 0.5 min, and then each sample plant was immersed in a 70% ethanol solution for 0.8 min, washed with sterile water (rinsed 3 times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water 5 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0080] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0081] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 25°C and 110r / min for 3 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0082] Example 2

[0083] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0084] Preparation of PDB medium: Boil 200g of potatoes (peeled) in boiling water for 30min, filter with gauze, add 20g of glucose, add water to make up to 1L, put into electric pressure steam sterilizer at 121℃ for 30min, divide into conical flasks for later use, the capacity of the conical flask is 150ml;

[0085] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 0.8 min, and then each sample plant was immersed in a 70% ethanol solution for 0.9 min, washed with sterile water (rinsed three times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water for 6 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0086] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0087] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 26°C and 116 r / min for 4 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0088] Example 3

[0089] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0090] Preparation of PDB medium: Boil 200g of potatoes (peeled) in boiling water for 30min, filter with gauze, add 20g of glucose, add water to make up to 1L, put into electric pressure steam sterilizer at 121℃ for 30min, divide into conical flasks for later use, the capacity of the conical flask is 150ml;

[0091] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 1 minute, and then each sample plant was immersed in a 70% ethanol solution for 1 minute, washed with sterile water (rinsed three times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water for 6 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0092] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0093] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 28°C and 120r / min for 5 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0094] Example 4

[0095] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0096] Preparation of PDB medium: Boil 200 g of potatoes (peeled) in boiling water for 30 min, filter with gauze, add 20 g of glucose and 0.05 g / L of caspofungin, add water to make up to 1 L, put it in an electric pressure steam sterilizer and sterilize it at 121°C for 30 min, divide it into conical flasks and set aside for later use. The capacity of the conical flask is 150 ml.

[0097] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 1 minute, and then each sample plant was immersed in a 70% ethanol solution for 1 minute, washed with sterile water (rinsed three times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water for 6 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0098] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0099] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 28°C and 120r / min for 5 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0100] Example 5

[0101] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0102] Preparation of PDB medium: Boil 200 g of potatoes (peeled) in boiling water for 30 min, filter with gauze, add 20 g of glucose and 0.1 g / L of caspofungin, add water to make up to 1 L, put into an electric pressure steam sterilizer and sterilize at 121°C for 30 min, divide into conical flasks and set aside for later use. The capacity of the conical flask is 150 ml.

[0103] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 1 minute, and then each sample plant was immersed in a 70% ethanol solution for 1 minute, washed with sterile water (rinsed three times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water for 6 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0104] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0105] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 28°C and 120r / min for 5 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0106] Example 6

[0107] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0108] Preparation of PDB medium: Boil 200 g of potatoes (peeled) in boiling water for 30 min, filter with gauze, add 20 g of glucose and 0.2 g / L of caspofungin, add water to make up to 1 L, put it in an electric pressure steam sterilizer and sterilize it at 121°C for 30 min, divide it into conical flasks and set aside for later use. The capacity of the conical flask is 150 ml.

[0109] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 1 minute, and then each sample plant was immersed in a 70% ethanol solution for 1 minute, washed with sterile water (rinsed three times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water for 6 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0110] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0111] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 28°C and 120r / min for 5 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0112] Example 7

[0113] Sampling: Orange-yellow Jade Phoenix, collected from Xiangtoushan Nature Reserve (114°E, 23°N, 460m above sea level);

[0114] Preparation of PDB medium: Boil 200 g of potatoes (peeled) in boiling water for 30 min, filter with gauze, add 20 g of glucose and 0.3 g / L of caspofungin, add water to make up to 1 L, put it in an electric pressure steam sterilizer at 121°C for 30 min, divide it into conical flasks for later use, and the capacity of the conical flask is 150 ml;

[0115] The collected roots of the orange-yellow Jade were rinsed with running water to clean the sand and other particles on the surface of the rhizomes of the orange-yellow Jade. The rhizomes of the orange-yellow Jade were selected and cut with scissors and placed in a fixed cup, and then moved to a clean bench. The rhizomes of the orange-yellow Jade were cut into small sections of 1.5±0.5 cm each, and then immersed in a 2.5% sodium hypochlorite solution for 1 minute, and then each sample plant was immersed in a 70% ethanol solution for 1 minute, washed with sterile water (rinsed three times), and the surface of the rhizomes was gently scraped clean with a sterilized inoculation needle, rinsed with sterile water for 6 times, and placed on sterile absorbent paper to dry, to obtain new nutrient roots;

[0116] Place the newly grown nutrient roots in a culture dish filled with sterile water, and use a sterilized inoculation needle to scrape out a single hyphae mass from the newly grown nutrient roots;

[0117] On the clean bench, use a sterilized pipette and bamboo stick to pick up the single mycelium mass of the newly formed nutrient root into the PDB medium, and culture it on a constant temperature shaker at 28°C and 120r / min for 5 days. Use sterilized pliers and scissors to cut the mycelium ball in the PDB medium into pieces, and use a vortex oscillator to evenly mix the mycelium and the bacterial solution to make a golden thread vine root growth-promoting solution.

[0118] The roxburghii root growth promoting liquid obtained in Examples 1 to 7 was used for the cultivation of roxburghii plants.

[0119] 1. Materials:

[0120] 1.1 The plants of Anoectochilus roxburghii with the same growth potential are derived from the tissue culture seedlings of wild Anoectochilus roxburghii in Longmen provided by Longmen County Hujinyuan Ecological Agriculture Development Co., Ltd.;

[0121] 1.2 Cultivation medium: peat soil;

[0122] 2. Culture conditions:

[0123] The cultivation experiment of Anoectochilus roxburghii was carried out in the shaded greenhouse of Hujinyuan cultivation base in Longmen County, Huizhou City, which is located in the tropical monsoon climate zone. Because it is located in a low-latitude area and close to the South China Sea, the climate is affected by the ocean and shows typical marine characteristics. The annual average temperature is stable at 23°C, and there is almost no frost all year round, for more than 350 days. The annual rainfall is about 1,770 mm, concentrated in the period from April to September;

[0124] 3. Test methods:

[0125] 3.1 A completely randomized design was adopted, with 10 roxburghii seedlings as one group, and 3 groups of roxburghii seedlings as one group, which were numbered for a total of 8 groups, with the 8th group being the blank control group;

[0126] 3.2 The usage of the golden thread lotus root growth promoting liquid is as follows:

[0127] The roxburghii tissue culture seedlings of groups 1 to 7 were treated with the roxburghii root growth promoting liquid of Examples 1 to 7 respectively;

[0128] For each of the 1st to 7th groups: the amount of the golden thread vine root growth promoting liquid used in the golden thread vine tissue culture seedlings of the first group is 5 ml; the amount of the golden thread vine root growth promoting liquid used in the golden thread vine tissue culture seedlings of the second group is 10 ml; the amount of the golden thread vine root growth promoting liquid used in the golden thread vine tissue culture seedlings of the third group is 15 ml;

[0129] Group 8: Each group of A. roxburghii tissue culture seedlings uses the same amount of sterile water as each group of A. roxburghii tissue culture seedlings in other groups;

[0130] 3.3 Three holes were made on the culture medium of each roxburghii seedling, all of which were located around the root system of the roxburghii seedling. The corresponding amount of roxburghii root growth-promoting liquid for each roxburghii seedling was poured into the corresponding hole, and then the hole was filled and leveled. The spider lilies were collected after they grew 150 meters.

[0131] 4. Measurement method:

[0132] The root length of each group of A. roxburghii tissue culture seedlings was measured and recorded; and the biomass of A. roxburghii plants in each group was measured and recorded: the drying method was used, and the temperature was 105°C for 2 hours to constant weight. After drying, the dry weight of the A. roxburghii spider was weighed, and the biomass and water content of the A. roxburghii plant were calculated using the formula.

[0133] 5. Measurement results:

[0134] 5.1 Table 1: Root length of Anoectochilus roxburghii tissue culture seedlings (unit: cm)

[0135]

[0136]

[0137] 5.2 Table 2: Water content of the root system of the tissue culture seedlings of Anoectochilus roxburghii (unit: %)

[0138]

[0139]

[0140] 5.3 Table 3: Content of roxburghii glycoside in tissue culture seedlings of Anoectochilus roxburghii (unit: %; average value)

[0141]

[0142]

[0143] As can be seen from Table 1, the root length of the golden thread lily tissue culture seedlings treated with the golden thread lily root growth-promoting liquid is significantly longer than that of the golden thread lily tissue culture seedlings not treated with the golden thread lily root growth-promoting liquid, indicating that the golden thread lily root growth-promoting liquid has a positive promoting effect on the growth of the root system of the golden thread lily tissue culture seedlings; as can be seen from Table 2, the water content of the roots of the golden thread lily tissue culture seedlings treated with the golden thread lily root growth-promoting liquid is not significantly different from that of the roots of the golden thread lily tissue culture seedlings not treated with the golden thread lily root growth-promoting liquid, indicating that the water absorption of the roots of the golden thread lily tissue culture seedlings treated with the golden thread lily root growth-promoting liquid is not significantly different from that of the roots of the golden thread lily tissue culture seedlings No effect, and thus no effect on the overall growth of the Anoectochilus roxburghii tissue culture seedlings; as can be seen from Table 3, the anoectochiloside content of the Anoectochilus roxburghii tissue culture seedlings treated with the Anoectochilus roxburghii root growth-promoting liquid was significantly increased compared with the anoectochiloside content of the Anoectochilus roxburghii tissue culture seedlings not treated with the Anoectochilus roxburghii root growth-promoting liquid, indicating that the Anoectochilus roxburghii root growth-promoting liquid has a positive promoting effect on the anoectochiloside content of the Anoectochilus roxburghii tissue culture seedlings; further, as can be seen from Tables 1 and 2, the Anoectochilus roxburghii root growth-promoting liquid of Examples 4 to 7 has a more obvious positive promoting effect on the growth of the root system and the increase in the anoectochiloside content.

[0144] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for promoting root growth of Anoectochilus roxburghii, characterized in that: Using a root growth-promoting liquid for roxburghii to perform root irrigation on roxburghii plants, so that the root growth-promoting liquid for roxburghii can infiltrate the roots of the roxburghii plants; Wherein, the anoectochilus root growth promoting liquid is the endophytic fungus culture liquid of the orange-yellow jade flower.

2. The method for promoting root growth of Anoectochilus roxburghii according to claim 1, characterized in that: The root irrigation operation of the golden thread lily plant is performed using the golden thread lily root growth promoting liquid, which includes the following steps: Obtaining the roxburghii roxburghii plant; Performing a cultivation treatment on the roxburghii roxburghii plant so that the roxburghii roxburghii plant is planted in a cultivation medium; Punching the cultivation substrate to form a plurality of holes on the cultivation substrate, each of the holes being arranged adjacent to the root system of the roxburghii plant; The borehole is irrigated with the roxburghii root growth-promoting liquid so that the roxburghii root growth-promoting liquid can flow back into the borehole.

3. The method for promoting root growth of Anoectochilus roxburghii according to claim 2, characterized in that: After the step of using the roxburghii root growth promoting liquid to fill the borehole, the method for promoting the root growth of roxburghii also includes the following steps: The drill hole is filled and leveled so that the cultivation substrate can level the drill hole.

4. The method for promoting root growth of Anoectochilus roxburghii according to claim 2, characterized in that: The cultivation medium is peat soil.

5. The method for promoting root growth of Anoectochilus roxburghii according to claim 1, characterized in that: The steps of obtaining the golden thread lily root system growth promoting liquid are as follows: Pick wild orange-yellow hibiscus flowers; Performing rhizome treatment on the orange-yellow jade flower to clean the surface of the rhizome of the orange-yellow jade flower and obtain new nutrient roots that form mycorrhizae; Scraping the bacterial mass from the newly-born nutrient root to absorb the bacterial mass on the newly-born nutrient root to obtain the orange-yellow jade flower bacterial community; The acanthocephalum fungus community is subjected to a proliferation and cultivation operation to obtain the roxburghii root system growth-promoting liquid.

6. The method for promoting root growth of Anoectochilus roxburghii according to claim 5, characterized in that: The rhizome treatment of the orange-yellow hibiscus flower specifically comprises the following steps: The rhizome of the orange-yellow hibiscus flower is washed and cut to remove particles on the surface of the rhizome of the orange-yellow hibiscus flower, thereby obtaining the rhizome segments of the orange-yellow hibiscus flower; Deeply cleaning the rhizome segments of the orange-yellow jasmine to remove bacteria and harmful substances on the surface of the rhizome segments of the orange-yellow jasmine; The orange-yellow Jade Flower rhizome segment after deep cleaning is cleaned to remove liquid on the surface of the orange-yellow Jade Flower rhizome segment to obtain new nutrient roots.

7. The method for promoting root growth of Anoectochilus roxburghii according to claim 6, characterized in that: The rhizome of the orange-yellow jasmine flower is washed and cut, and the specific operation is as follows: The rhizome of the orange-yellow jasmine flower is rinsed under running water, and then the rhizome of the orange-yellow jasmine flower is cut into segments to obtain the rhizome segments of the orange-yellow jasmine flower.

8. The method for promoting root growth of Anoectochilus roxburghii according to claim 6 or 7, characterized in that: The length of the rhizome section of the orange-yellow Jade Phoenix flower is 1 cm to 2 cm.

9. The method for promoting root growth of Anoectochilus roxburghii according to claim 6, characterized in that: The rhizome segment of the orange-yellow jasmine flower is subjected to a deep cleaning treatment, and the specific operation is as follows: The rhizome segments of the orange-yellow jasmine flower are immersed in a sodium hypochlorite solution, and then taken out and immersed in an ethanol solution.

10. The method for promoting root growth of Anoectochilus roxburghii according to claim 6, characterized in that: The orange-yellow Jade Phoenix rhizome segment after deep cleaning is cleaned, and the specific operation is as follows: The rhizome segment of the orange-yellow jasmine flower is rinsed with sterile water for at least three times, and then the liquid on the surface of the rhizome segment of the orange-yellow jasmine flower is absorbed with sterile absorbent paper.

Images