Tooth remineralization gel and preparation method thereof

Abstract

The invention belongs to the technical field of oral care gel, and discloses tooth remineralization gel and a preparation method thereof. The invention discloses a tooth remineralization gel. The tooth remineralization gel contains sodium fluoride, casein phosphopeptide-amorphous calcium phosphate and biological polyphenol. The cleaning agent specifically comprises the following components: sodium fluoride, casein phosphopeptide-amorphous calcium phosphate, triethanolamine, biological polyphenol, glycerol, carbomer and the balance of water. The sodium fluoride and the casein phosphopeptide-amorphous calcium phosphate are combined for use, so that the poisoning risk of the sodium fluoride is reduced, and the remineralization effect is improved.

Description

Technical Field

[0001] The invention belongs to the field of oral care gel, and in particular relates to a tooth remineralization gel and a preparation method thereof. Background Art

[0002] The prevalence of dental caries in the population is extremely high. According to foreign research data, the prevalence of dental caries is close to 72.9%. The results of the fourth national oral health epidemiological survey in my country show that the dental caries rates in the 5-year-old, 35-44-year-old, and 55-64-year-old age groups are 71.9%, 89.0%, and 95.6%, respectively. Children mainly like to eat sweets, do not brush their teeth enough, and have poor oral hygiene. They should pay attention to eating less sweets, brush their teeth carefully in the morning and evening under the guidance of their parents, and use remineralization products for prevention.

[0003] The general awakening of the public's awareness of beauty and the steady growth of national income have led to an increase in the public's demand for teeth whitening. However, whitening can damage teeth and cause tooth demineralization, so remineralization treatment is still necessary. Remineralization gel is relatively cheap and convenient compared to dental treatment. You can find a professional dentist to apply remineralization treatment, which is faster and more direct. You can also take it home and apply it according to the instructions. It is easy to use and has a quicker effect.

[0004] At present, the remineralization materials include: ① simple fluoride ② amelogenin ③ non-amelogenin ④ amelogenin peptide ⑤ casein phosphopeptide-amorphous calcium phosphate ⑥ calcium sodium phosphosilicate ⑦ tricalcium phosphate. At present, the most used is simple fluoride.

[0005] The use of simple fluoride for treatment has unstable content and excessive dosage can cause poisoning reactions; the cost of amelogenin series is high and the production process is complicated; the remineralization of casein phosphopeptide-amorphous calcium phosphate is difficult to achieve ideal results and needs to be used in combination with fluoridating agent laser; calcium sodium phosphosilicate and tricalcium phosphate have the risk of tartar formation. Summary of the invention

[0006] In view of the above-mentioned problems existing in the current remineralization gel, the present invention aims to provide a tooth remineralization gel and a preparation method thereof, which can reduce the risk of sodium fluoride poisoning and improve the remineralization effect.

[0007] A tooth remineralization gel, comprising sodium fluoride, casein phosphopeptide-amorphous calcium phosphate and biopolyphenols.

[0008] Furthermore, the invention specifically comprises the following components: 0.1-0.5wt.% sodium fluoride, 0.1-3wt.% casein phosphopeptide-amorphous calcium phosphate, 0.5-5wt.% triethanolamine, 1.8-2.3wt.% biopolyphenols, 8-20wt.% glycerol, 0.5-4wt.% carbomer, and the balance is made up of water.

[0009] Furthermore, the biological polyphenol is one of brown algae polyphenol and sea grape polyphenol, or a mixture of the two in any ratio.

[0010] A method for preparing a tooth remineralization gel, the method comprising the following steps:

[0011] (1) dissolving casein phosphopeptide in sodium phosphate buffer and stirring, adding CaCl2 solution dropwise to prepare casein phosphopeptide-amorphous calcium phosphate solution; then adding NaF solution thereto and mixing evenly, adding triethanolamine to obtain mixed solution A;

[0012] (2) adding biopolyphenols to the mixed solution A in batches and stirring to obtain a mixed solution B;

[0013] (3) Add glycerol, carbomer and purified water into mixed solution B and stir to obtain a tooth remineralization gel.

[0014] Furthermore, in step (1), the concentration of the CaCl2 solution is 0.5-4 mol / L, the pH of the sodium phosphate buffer is 6.8-7.2, the mass concentration of the obtained casein phosphopeptide-amorphous calcium phosphate solution is 1-5%, and the mass concentration of the NaF solution is 0.1-0.5%.

[0015] Furthermore, after adding triethanolamine in step (1), stirring is performed at 200-400 rpm for 2-6 hours.

[0016] Furthermore, the process of adding in batches in step (2) is as follows: by mass fraction, 20% of the amount of bio-polyphenols is added for the first time and stirred for 2 hours, 50% of the amount of bio-polyphenols is added for the second time and stirred for 8-16 hours, and 30% of the amount of bio-polyphenols is added for the third time and stirred for 2-3 hours. Adding in batches facilitates uniform mixing.

[0017] Furthermore, the stirring condition in step (3) is: stirring at 300-1000 rpm for 4-8 hours.

[0018] Compared with the prior art, the advantages of the present invention are:

[0019] The present invention adopts sodium fluoride and casein phosphopeptide-amorphous calcium phosphate in combination, which not only reduces the poisoning risk of sodium fluoride, but also has better remineralization effect than single sodium fluoride or casein phosphopeptide-amorphous calcium phosphate; the added biological polyphenols can inhibit oral bacteria and indirectly participate in the process of tooth remineralization; in addition, the present invention has low raw material cost, simple preparation and convenient use. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 These are pictures of the Example 1 group after demineralization, the first week of remineralization, and the second week of remineralization;

[0021] Figure 2 The pictures are of the gel group obtained after demineralization, after the first week of remineralization, and after the second week of remineralization, which are basically the same as those in Example 1 but omitting the biopolyphenol component;

[0022] Figure 3 These are pictures of the sodium fluoride aqueous solution group after demineralization, the first week of remineralization, and the second week of remineralization;

[0023] Figure 4 These are the pictures of the purified water group before demineralization, after demineralization, and after the first week of remineralization;

[0024] Figure 5 These are pictures of the untreated group before demineralization, after demineralization, and after the first week of remineralization. DETAILED DESCRIPTION

[0025] The following provides a specific embodiment of a tooth remineralization gel and a preparation method thereof of the present invention. It is necessary to point out that the following examples are only used to illustrate the present invention in more detail, rather than to narrow the scope of protection of the present invention. After reading the present invention, improvements and adjustments made by professionals in this technical field without departing from the concept of the present invention are within the scope of protection claimed by the present invention.

[0026] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0027] A tooth remineralization gel comprises the following components: 0.1-0.5wt.% sodium fluoride, 0.1-3wt.% casein phosphopeptide-amorphous calcium phosphate, 0.5-5wt.% triethanolamine, 1.8-2.3wt.% biopolyphenols, 8-20wt.% glycerol, 0.5-4wt.% carbomer, and the balance is made up of water.

[0028] A method for preparing a tooth remineralization gel, the method comprising the following steps:

[0029] (1) dissolving casein phosphopeptide in sodium phosphate buffer and stirring, adding CaCl2 solution dropwise to prepare casein phosphopeptide-amorphous calcium phosphate solution; then adding NaF solution thereto and mixing evenly, adding triethanolamine to obtain mixed solution A;

[0030] (2) adding biopolyphenols to the mixed solution A in batches and stirring to obtain a mixed solution B;

[0031] (3) Add glycerol, carbomer and purified water into mixed solution B and stir to obtain a tooth remineralization gel.

[0032] Furthermore, in step (1), the concentration of the CaCl2 solution is 0.5-4 mol / L, the pH of the sodium phosphate buffer is 6.8-7.2, the mass concentration of the obtained casein phosphopeptide-amorphous calcium phosphate solution is 1-5%, and the mass concentration of the NaF solution is 0.1-0.5%.

[0033] Furthermore, after adding triethanolamine in step (1), stirring is performed at 200-400 rpm for 2-6 hours.

[0034] Furthermore, the process of adding in batches in step (2) is as follows: by mass fraction, 20% of the amount of biopolyphenols is added for the first time and stirred for 2 hours, 50% of the amount of biopolyphenols is added for the second time and stirred for 8-16 hours, and 30% of the amount of biopolyphenols is added for the third time and stirred for 2-3 hours.

[0035] Furthermore, the stirring condition in step (3) is: stirring at 300-1000 rpm for 4-8 hours.

[0036] Example 1

[0037] A tooth remineralization gel comprises the following components: 0.2wt.% sodium fluoride, 2wt.% casein phosphopeptide-amorphous calcium phosphate, 2wt.% triethanolamine, 2wt.% biopolyphenol, 12wt.% glycerol, 3wt.% carbomer, and the balance is made up of water.

[0038] A method for preparing a tooth remineralization gel, the method comprising the following steps:

[0039] (1) dissolving casein phosphopeptide in sodium phosphate buffer and stirring, adding CaCl2 solution dropwise to prepare casein phosphopeptide-amorphous calcium phosphate solution; then adding NaF solution thereto and mixing evenly, adding triethanolamine to obtain mixed solution A;

[0040] (2) adding biopolyphenols to the mixed solution A in batches and stirring to obtain a mixed solution B;

[0041] (3) Add glycerol, carbomer and purified water into mixed solution B and stir to obtain a tooth remineralization gel.

[0042] Furthermore, in step (1), the concentration of the CaCl2 solution is 2 mol / L, the pH of the sodium phosphate buffer is 7, the mass concentration of the obtained casein phosphopeptide-amorphous calcium phosphate solution is 2%, and the mass concentration of the NaF solution is 0.2%.

[0043] Furthermore, after adding triethanolamine in step (1), stirring was carried out at 300 rpm for 4 hours.

[0044] Furthermore, the process of adding in batches in step (2) is as follows: by mass fraction, 20% of the amount of biopolyphenols is added for the first time and stirred for 2 hours, 50% of the amount of biopolyphenols is added for the second time and stirred for 10 hours, and 30% of the amount of biopolyphenols is added for the third time and stirred for 3 hours.

[0045] Furthermore, the stirring condition in step (3) is: stirring at 700 rpm for 6 hours.

[0046] The remineralization gel prepared in Example 1 was tested on teeth

[0047] ① Establishment of demineralization model

[0048] Prepare solutions of 0.1 mol / L lactic acid and 30 g / L carboxymethyl cellulose respectively, take 100 mL of each and mix them evenly to form a demineralizing solution.

[0049] Five groups of experiments were conducted: Example 1 group, gel group obtained by omitting the biopolyphenol component in Example 1, 2% sodium fluoride aqueous solution group, purified water group, and untreated group (after demineralization). The ex vivo human teeth of each group were simultaneously immersed in the demineralization solution for 96 hours.

[0050] ②Remineralization effect

[0051] After the demineralization soaking is completed, each group of human teeth is soaked or otherwise treated according to different requirements.

[0052] The test shows that: after two weeks of immersion remineralization treatment, the white spots on the tooth surface of the Example 1 group (remineralization gel group) disappeared, and the remineralization effect was obvious. After two weeks of immersion remineralization treatment in the omitted biopolyphenol group, there was a remineralization effect, but the effect was not good. After two weeks of immersion remineralization treatment in the sodium fluoride aqueous solution group, there was no obvious change in the white spots on the tooth surface, and there was no remineralization effect. After one week of immersion remineralization in the purified water group, there was no obvious change in the white spots on the tooth surface, and there was no remineralization effect. In the untreated group, after demineralization and standing, there was no obvious change in the white spots on the tooth surface, and there was no remineralization effect.

[0053] Note: The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention; therefore, although this specification has described the present invention in detail with reference to the above embodiments, a person skilled in the art should understand that the present invention can still be modified or replaced by equivalents; and all technical solutions and improvements thereof that do not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A tooth remineralization gel, characterized in that: Contains: sodium fluoride, casein phosphopeptide-amorphous calcium phosphate and biopolyphenols.

2. The tooth remineralization gel according to claim 1, characterized in that The invention comprises the following components: 0.1-0.5wt.% sodium fluoride, 0.1-3wt.% casein phosphopeptide-amorphous calcium phosphate, 0.5-5wt.% triethanolamine, 1.8-2.3wt.% biopolyphenol, 8-20wt.% glycerol, 0.5-4wt.% carbomer, and the balance is made up of water.

3. The tooth remineralization gel according to claim 1 or 2, characterized in that The biological polyphenol is one of brown algae polyphenol and sea grape polyphenol or a mixture of the two in any ratio.

4. A method for preparing a tooth remineralization gel according to any one of claims 1 to 3, characterized in that: The preparation method comprises the following steps: (1) dissolving casein phosphopeptide in sodium phosphate buffer and stirring, and adding CaCl2 solution dropwise to prepare casein phosphopeptide-amorphous calcium phosphate solution; Then, NaF solution is added thereto and mixed evenly, and then triethanolamine is added to obtain a mixed solution A; (2) adding biopolyphenols to the mixed solution A in batches and stirring to obtain a mixed solution B; (3) Add glycerol, carbomer and purified water into mixed solution B and stir to obtain a tooth remineralization gel.

5. The method for preparing the tooth remineralization gel according to claim 4, characterized in that: In the step (1), the concentration of the CaCl2 solution is 0.5-4 mol / L, the pH of the sodium phosphate buffer is 6.8-7.2, the mass concentration of the prepared casein phosphopeptide-amorphous calcium phosphate solution is 1-5%, and the mass concentration of the NaF solution is 0.1-0.5%.

6. The method for preparing the tooth remineralization gel according to claim 4, characterized in that: After adding triethanolamine in step (1), stirring is performed at 200-400 rpm for 2-6 hours.

7. The method for preparing the tooth remineralization gel according to claim 4, characterized in that: The process of adding in batches in step (2) is as follows: by mass fraction, 20% of the biopolyphenols is added for the first time and stirred for 2 hours, 50% of the biopolyphenols is added for the second time and stirred for 8-16 hours, and 30% of the biopolyphenols is added for the third time and stirred for 2-3 hours.

8. The method for preparing the tooth remineralization gel according to claim 4, characterized in that: The stirring condition in step (3) is: stirring at 300-1000 rpm for 4-8 hours.

Images