Anti-adherence micro-fluidic chip based on interface suspension method, system and use method

Abstract

The invention relates to an anti-adherence micro-fluidic chip based on an interface suspension method, and a system and a use method thereof. The micro-fluidic chip comprises an upper substrate, a lower substrate, an inner phase flow channel, a shear phase flow channel, a liquid drop transportation flow channel, a liquid drop suspension auxiliary flow channel and a stepped structure. The inner phase flow channel and the shear phase flow channel form a flow focusing structure, and a circle of flow protection layer is formed on the inner wall of the liquid drop conveying flow channel through the auxiliary phase solution, so that the liquid drops are still bound on an interface even under the interference of gravity or other forces and are kept in a suspension state, and the liquid drops are prevented from being wetted, attached to the wall and hung on the wall. Through structure adjustment and auxiliary phase fluid protection, liquid drop suspension type transportation is achieved, the surface wetting and wall hanging phenomena in the microsphere generation process are effectively prevented, and the device has the advantages of being easy to manufacture, low in cost, capable of being repeatedly used and the like and is suitable for being applied to microfluidics in the fields of biomedicine, chemical analysis and the like.

Description

Technical Field

[0001] The present invention belongs to the field of microfluidic chips, and in particular relates to an anti-adhesion microfluidic chip based on an interface suspension method, a system and a use method. Background Art

[0002] Microfluidics technology has important applications in biomedicine, chemical analysis, and environmental monitoring. Its core equipment, microfluidic chips, are manufactured on a micrometer scale through precision machining technology, and can achieve precise manipulation of liquids in tiny channels. Microsphere generation technology is an important application of microfluidic chips and is widely used in drug delivery, cell culture, sensor manufacturing, and other fields. However, when the flow channel placement direction forms a certain angle with the gravity direction, the effect of gravity will cause the microspheres to deviate toward the wall in the flow channel. If the microspheres contact the wall for too long, they will wet the wall and hang on the wall. During the microsphere generation process, surface wetting and hanging on the wall will cause the microspheres to adhere to the flow channel wall of the chip, thereby affecting the morphology, size uniformity, and collection efficiency of the microspheres. Therefore, the pretreatment of the chip flow channel wall has always been an important issue in the field of microfluidics. In order to deal with the surface wetting of droplets in the flow channel, a layer of hydrophobic microstructure similar to the surface of a lotus leaf is usually processed in the flow channel, or a hydrophobic material is attached to the flow channel surface. Although both methods can effectively improve droplet wetting, they have certain limitations. First, it is difficult to process the microstructure, and it is also very difficult to clean this type of chip after use. There may be residual solution in the microstructure, affecting subsequent use; secondly, the method of hydrophobic treatment of the flow channel surface is adopted. This method reduces the surface energy by coating a layer of hydrophobic material on the surface of the chip, thereby reducing the adhesion between the droplets and the flow channel wall. However, the adhesion effect of the hydrophobic coating in the flow channel is poor. This hydrophobic coating is easy to fall off during long-term use, resulting in a decrease in effect. Long-term scouring and scraping of droplets will damage the coating, and it is difficult to use for a long time. Summary of the invention

[0003] 1. Technical problems to be solved: How to pre-treat the chip channel wall to avoid droplet wetting and ensure long-term use.

[0004] 2. Technical solution: In order to solve the above problems, the present invention provides an anti-adherence microfluidic chip based on the interface suspension method, including a substrate, the substrate including an upper substrate and a lower substrate, the lower part of the upper substrate and the upper part of the lower substrate are both provided with concave shapes, and when the upper substrate and the lower substrate are combined, the two concave shapes form a flow channel, the flow channel includes an inner phase flow channel, a shear phase flow channel, a droplet suspension auxiliary phase flow channel, and a droplet transport flow channel, the inner phase flow channel, the shear phase flow channel, and the droplet suspension auxiliary phase flow channel are all provided with a solution inlet, and the droplet transport flow channel A flow channel outlet is provided, and the internal phase flow channel and the droplet transport flow channel are perpendicular to the droplet suspension auxiliary flow channel and are connected to each other. There are two shear phase flow channels, which are located on both sides of the internal phase flow channel and are symmetrical with respect to the internal phase flow channel. The shear phase flow channels on both sides converge at the internal phase flow channel. The solution passed into the internal phase flow channel, the solution in the shear phase flow channel and the solution in the droplet suspension auxiliary phase flow channel are insoluble in each other, and the interfacial tension between the solution passed into the droplet suspension auxiliary phase flow channel and the solution passed into the shear phase flow channel is greater than the gravity of the droplets.

[0005] A stepped structure is provided in the substrate, specifically: the flow channel height of the inner phase flow channel must be higher than the flow channel height of the shear phase flow channel, the flow channel height of the shear phase flow channel is higher than the flow channel height of the droplet suspension auxiliary phase flow channel, and the flow channel height of the droplet suspension auxiliary phase flow channel is higher than the flow channel height of the droplet transport flow channel.

[0006] The inner phase flow channel is fed with a sodium alginate solution, the shear phase flow channel is fed with liquid paraffin containing a spam, and the droplet suspension auxiliary phase flow channel is fed with glycerol.

[0007] Pure water is introduced into the inner phase flow channel, dimethyl silicone oil is introduced into the shear phase flow channel, and fluorinated oil is introduced into the droplet suspension auxiliary phase flow channel.

[0008] Pure water is introduced into the inner phase flow channel, peanut oil is introduced into the shear phase flow channel, and dimethyl silicone oil is introduced into the droplet suspension auxiliary phase flow channel.

[0009] The inner phase solution enters the inner phase flow channel through the inner phase flow channel inlet, the shear phase solution enters the shear phase flow channel through the shear phase flow channel inlet, a rectangular inlet of the auxiliary phase flow channel is provided at the intersection of the inner phase flow channel, the droplet transport flow channel and the droplet suspension auxiliary phase flow channel, the auxiliary phase solution enters the droplet suspension auxiliary phase flow channel through the rectangular inlet of the auxiliary phase flow channel and the droplet suspension auxiliary phase flow channel circular hole inlets provided at both ends of the droplet suspension auxiliary phase flow channel, the rectangular inlet of the auxiliary phase flow channel is connected to the inlet end of the droplet transport flow channel. The inner phase flow channel inlet, the shear phase flow channel inlet and the droplet suspension auxiliary phase flow channel circular hole inlet pass through the upper substrate, and the rectangular inlet of the auxiliary phase flow channel passes through the upper substrate and the lower substrate.

[0010] The flow cross-section shapes of the inner phase flow channel, the shear phase flow channel and the droplet suspension auxiliary phase flow channel may be circular or rectangular.

[0011] The upper substrate and the lower substrate are made of transparent material.

[0012] The present invention also provides an anti-adhesion microfluidic chip system based on the interface suspension method, wherein the inner phase flow channel inlet, the shear phase flow channel inlet, the auxiliary phase flow channel rectangular inlet and the droplet suspension auxiliary phase flow channel circular hole inlet are respectively connected to the syringe through a hose, the syringe is placed on a syringe pump with adjustable flow rate, the flow channel outlet is connected to a collection beaker through a hose, and a microscope imaging device is used to observe the droplet transport flow channel condition.

[0013] The present invention also provides a method for using an anti-adhesion microfluidic chip based on an interface suspension method, comprising the following steps: Step 1: A solution that is insoluble in the inner and shear phases and has a large interfacial tension with the shear phase solution is introduced into the circular hole inlet of the droplet suspension auxiliary phase flow channel and the rectangular inlet of the auxiliary phase flow channel to fill the droplet transport flow channel in advance; Step 2: injecting shear phase solution into the inlet of the shear phase flow channel, adjusting the flow rate ratio of the shear phase and the auxiliary phase, forming a stable interface channel, and forming a protective layer on the inner wall of the droplet transport flow channel; Step 3: Introduce the inner phase solution into the inner phase flow channel, adjust the flow rate, so that the inner phase solution is sheared into balls at the intersection of the inner phase flow channel and the shear phase flow channel, and flows out along the direction of the droplet transport flow channel. Under the influence of gravity, the droplets sink and are finally bound to the interface by the interfacial tension with the auxiliary phase, and are collected after flowing out together with the shear phase and auxiliary phase liquids.

[0014] 3. Beneficial effects: The present invention prevents droplets from adhering to the wall and wetting by adjusting the chip structure. The production is simple and the production cost, maintenance cost and process requirements are greatly reduced. The step structure in the present invention indirectly adjusts the spatial position of droplet generation, and even if the droplets are deformed, it is difficult for them to contact the inner wall of the flow channel and wet. The present invention adds an auxiliary phase flow channel for droplet suspension. When the droplets are moved to the surrounding space by gravity or other interferences, the droplets will float on the flow interface formed by the shear phase and the auxiliary phase for transportation, achieving the effect of suspension, and it is difficult to break through the auxiliary phase solution and contact the inner wall to wet the flow channel. The chip can be reused, reducing operating costs.

[0015] The present invention is also applicable to the preparation of double-emulsion microspheres of micrometer size, and the suspension flow of microparticles of different sizes can be achieved by changing the flow channel size and the flow rate ratio of the injection pump. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a structural diagram of an anti-adhesion microfluidic chip based on an interface suspension method according to an embodiment of the present invention.

[0017] Figure 2 yes Figure 1An oblique view of the upper substrate in the structure shown.

[0018] Figure 3 yes Figure 1 Side view of the chip showing the relationship of shear droplet positions.

[0019] Figure 4 It is a cross-sectional schematic diagram of the droplet transport channel of the microfluidic chip transporting droplets.

[0020] Figure 5 It is a schematic diagram of how the microdroplets gradually deviate toward the wall after being affected by gravity, and finally suspend at the interface between the shear phase and the auxiliary phase for continued transportation.

[0021] Figure 6 It is a schematic diagram of the observation area of ​​an optical microscope.

[0022] Figure 7 Schematic diagram of the anti-adhesion microfluidic system based on the interfacial suspension method.

[0023] Explanation of the reference numerals: 1. Bonded substrate; 2. Internal phase flow channel; 3. Shear phase flow channel; 4. Auxiliary flow channel for droplet suspension; 5. Rectangular inlet of auxiliary phase flow channel; 6. Step structure; 7. Droplet transport flow channel; 8. Flow channel outlet; 9. Internal phase flow channel inlet; 10. Shear phase flow channel inlet; 11. Circular hole inlet of auxiliary phase flow channel for droplet suspension; 12. Microfluidic chip; 13. Injection pump; 14. Syringe; 15. Beaker; 16 Microscope imaging device. DETAILED DESCRIPTION

[0024] The present invention is described in detail below with reference to the accompanying drawings and embodiments.

[0025] like Figure 1 As shown, an anti-adhesion microfluidic chip based on the interface suspension method includes a substrate 1, wherein the substrate 1 includes an upper substrate and a lower substrate, and concave shapes are provided below the upper substrate and above the lower substrate. When the upper substrate and the lower substrate are closed, the two concave shapes form a flow channel.

[0026] In one embodiment, the microfluidic chip 12 can be engraved on a plate by an engraving machine and then bonded together; or a mold can be made using 3D printing technology and then cast with PDMS; or etching can be performed.

[0027] The flow channel includes an inner phase flow channel 2, a shear phase flow channel 3, a droplet suspension auxiliary phase flow channel 4, and a droplet transport flow channel 7. The function of the droplet suspension auxiliary phase flow channel 4 is that when the droplets move to the surrounding space due to gravity or other interference, the droplets will float on the flow interface formed by the shear phase and the auxiliary phase for transportation, thereby achieving a suspension effect.

[0028] The inner phase flow channel 2, the shear phase flow channel 3, and the droplet suspension auxiliary phase flow channel 4 are all provided with a solution inlet, and the droplet transport flow channel 7 is provided with a flow channel outlet 8. The inner phase flow channel 2 and the droplet transport flow channel 7 are perpendicular to the droplet suspension auxiliary flow channel 4 and communicate with each other. There are two shear phase flow channels 3, which are located on both sides of the inner phase flow channel 2 and are symmetrical relative to the inner phase flow channel 2. The shear phase flow channels 3 on both sides intersect at the inner phase flow channel 2 to form a flow channel focusing structure. At the same time, the shear phase solution shears the inner phase solution to form droplets, such as Figure 3 shown.

[0029] The solution passed into the inner phase flow channel 2, the solution passed into the shear phase flow channel 3 and the solution passed into the droplet suspension auxiliary phase flow channel 4 are not soluble in each other. The interfacial tension between the solution passed into the droplet suspension auxiliary phase flow channel 4 and the solution passed into the shear phase flow channel 3 is greater than the gravity of the droplets, and a protective layer with adjustable thickness will be formed in the liquid transport flow channel, so that the droplets flow out of the flow channel in a suspended manner, which greatly reduces the possibility of the droplets contacting the inner wall, thereby improving the stability of the droplet preparation process and the reliability of long-term operation.

[0030] like Figure 4 As shown in FIG. 1 , the auxiliary phase solution forms a 360° all-round protection in the flow channel; Figure 5 As shown in the figure, under the action of interference forces such as gravity and buoyancy, the microspheres gradually shift to the interface layer. Due to the interfacial tension between the two phases, they are bound to the interface, preventing the droplets from penetrating the interface, so that the microspheres are suspended above the interface for transportation.

[0031] In one embodiment, a sodium alginate solution with a mass fraction of 1% is passed into the inner phase flow channel 2, liquid paraffin containing 3% Span 80 is passed into the shear phase flow channel 3, and glycerol is passed into the droplet suspension auxiliary phase flow channel 4.

[0032] Liquid paraffin shears sodium alginate at the intersection of the flow channels and breaks it into droplets. Under the action of surface tension, the droplets become round. The size of the droplets mainly depends on the flow channel size, the two-phase flow rate and the properties of the two-phase liquid.

[0033] The auxiliary phase is introduced into glycerol through the circular hole inlet 11 of the droplet suspension auxiliary phase flow channel to cover the four walls of the droplet transport flow channel. The flow rates of the shear phase and the auxiliary phase are adjusted to control the thickness of the protective layer.

[0034] There is a large interfacial tension between glycerol and liquid paraffin, and sodium alginate solution is insoluble in glycerol and liquid paraffin. A protective layer with adjustable thickness will be formed in the liquid transport channel, so that the droplets flow out of the channel in a suspended manner, greatly reducing the possibility of the droplets contacting the inner wall, thereby improving the stability of the droplet preparation process and the reliability of long-term work.

[0035] In one embodiment, pure water is introduced into the inner phase flow channel 2 , dimethyl silicone oil is introduced into the shear phase flow channel 3 , and fluorinated oil is introduced into the droplet suspension auxiliary phase flow channel 4 .

[0036] In one embodiment, pure water is introduced into the inner phase flow channel 2, peanut oil is introduced into the shear phase flow channel 3, and dimethyl silicone oil is introduced into the droplet suspension auxiliary phase flow channel 4.

[0037] In one embodiment, a stepped structure 6 is provided in the substrate 1, specifically: the flow channel height of the inner phase flow channel 2 needs to be higher than the flow channel height of the shear phase flow channel 3, the flow channel height of the shear phase flow channel 3 is higher than the flow channel height of the droplet suspension auxiliary phase flow channel 4, and the flow channel height of the droplet suspension auxiliary phase flow channel 4 is higher than the flow channel height of the droplet transport flow channel 7.

[0038] The step structure 6 can adjust the spatial position of droplet generation. This situation often occurs in microfluidic chips. When the flow rate ratio is at a critical value or is disturbed, the internal phase is difficult to break into liquid, the head liquid is extremely unstable, and it will hydrophilically contact the wall to flow. The existence of the step structure can preset a layer of auxiliary phase liquid on the upper and lower walls where the droplets are generated, forming a dead angle at the step corner, which greatly reduces the phenomenon of wall-adhering flow when unstable.

[0039] In one embodiment, the flow cross-section shapes of the inner phase flow channel 2, the shear phase flow channel 3 and the droplet suspension auxiliary phase flow channel 4 can be circular, rectangular, etc., and their sizes are not limited.

[0040] In one embodiment, the inner phase solution enters the inner phase flow channel 2 through the inner phase flow channel inlet 9, the shear phase solution enters the shear phase flow channel 3 through the shear phase flow channel inlet 10, and the auxiliary phase solution enters the droplet suspension auxiliary phase flow channel 4 through the droplet suspension auxiliary phase flow channel circular hole inlet 11 and the auxiliary phase flow channel rectangular inlet 5 provided at both ends of the droplet suspension auxiliary phase flow channel 4. Figure 2 Shown A rectangular inlet 5 for the auxiliary phase flow channel is provided at the intersection of the inner phase flow channel 2, the droplet transport flow channel 7 and the droplet suspension auxiliary phase flow channel 4, and the rectangular inlet 5 for the auxiliary phase flow channel is connected to the inlet end of the droplet transport flow channel 7. The inner phase flow channel inlet 9, the shear phase flow channel inlet 10 and the droplet suspension auxiliary phase flow channel circular hole inlet 11 pass through the upper substrate, and the rectangular inlet 5 for the auxiliary phase flow channel passes through the upper substrate and the lower substrate, that is, the droplet suspension auxiliary phase flow channel has four inlets, two droplet suspension auxiliary phase flow channel circular hole inlets 11 and two auxiliary phase flow channel rectangular inlets 5.

[0041] The present invention also provides an anti-adhesion microfluidic chip system based on the interface suspension method, such as Figure 7As shown, the inner phase flow channel inlet 9, the shear phase flow channel inlet 10, the auxiliary phase flow channel rectangular inlet 5 and the droplet suspension auxiliary phase flow channel circular hole inlet 11 in the anti-adhesion microfluidic chip 12 based on the interface suspension method are respectively connected to the syringe 14 through Teflon hoses; the syringe 14 is placed on the injection pump 13 with adjustable flow rate, and the flow channel outlet 8 has a round bottom groove, which can be directly connected to one end of the round hose after bonding, and the other end of the round tube is connected to the collection beaker 15, and the droplet transport flow channel condition is observed by a microscope imaging device 16, as shown in FIG. Figure 6 shown.

[0042] according to Figure 7 The main structure of the anti-adherence microfluidic system based on the interface suspension method includes: a microfluidic chip 12, a syringe pump 13, a syringe 14, a beaker 15, and a microscope imaging device 16. The microfluidic chip 12 in the anti-adherence microfluidic system based on the interface suspension method is the above-mentioned anti-adherence microfluidic chip based on the interface suspension method; the syringe pump 13 pushes the syringe 14 to introduce phase liquid into each phase inlet, adjusts the flow rate to reach a stable state, observes the droplet generation and collects it.

[0043] The droplets sink under the action of gravity, and the interface formed by the auxiliary phase and the shear phase will restrain the sinking droplets, achieving the effect of droplet suspension, preventing them from adhering to the wall to form a flow, and finally entering the beaker 15 in a suspended state.

[0044] In one embodiment, the geometric shape of the anti-adhesion microfluidic chip based on the interfacial suspension method is not limited, and can be a thin cuboid, a thin cylinder, etc., and its manufacturing material can be a non-metallic material with good light transmittance, such as organic glass, glass, PDMS, etc., or an opaque material. Transparent materials are used for easy observation.

[0045] The present invention also provides a method for using an anti-adhesion microfluidic chip based on an interface suspension method. First, a solution that is insoluble in the inner and shear phases and has a large interface tension with the shear phase solution is introduced into the circular hole inlet 11 of the auxiliary phase flow channel for droplet suspension and the rectangular inlet 5 of the auxiliary phase flow channel to fill the flow channel in advance.

[0046] The auxiliary phase solution enters from four inlets, and the flow rate of each inlet must be kept consistent.

[0047] Then, the shear phase solution is injected into the inlet of the shear phase flow channel, and the flow rate ratio of the shear phase and the auxiliary phase is adjusted to form a stable interface channel and a protective layer on the inner wall of the transport flow channel.

[0048] Finally, the inner phase solution is introduced into the inner phase flow channel, and the flow rate is adjusted so that the inner phase solution is sheared into balls at the step and flows out along the transport flow channel. Under the influence of gravity, the droplets sink and are eventually bound to the interface by the interfacial tension with the auxiliary phase. They flow out together with the shear phase and auxiliary phase liquid and are collected in a beaker.

Claims

1. An anti-adhesion microfluidic chip based on an interfacial suspension method, comprising a substrate (1), characterized in that: The substrate (1) comprises an upper substrate and a lower substrate. A concave shape is provided below the upper substrate and above the lower substrate. When the upper substrate and the lower substrate are closed, the two concave shapes form a flow channel. The flow channel comprises an internal phase flow channel (2), a shear phase flow channel (3), a droplet suspension auxiliary phase flow channel (4), and a droplet transport flow channel (7). The internal phase flow channel (2), the shear phase flow channel (3), and the droplet suspension auxiliary phase flow channel (4) are all provided with a solution inlet. The droplet transport flow channel (7) is provided with a flow channel outlet (8). The internal phase flow channel (2), the droplet transport flow channel ( 7) are perpendicular to the droplet suspension auxiliary flow channel (4) and are connected to each other. There are two shear phase flow channels (3), which are located on both sides of the internal phase flow channel (2) and are symmetrical with respect to the internal phase flow channel (2). The shear phase flow channels (3) on both sides converge at the internal phase flow channel (2). The solution passed into the internal phase flow channel (2), the solution passed into the shear phase flow channel (3) and the solution passed into the droplet suspension auxiliary phase flow channel (4) are insoluble in each other. The interfacial tension between the solution passed into the droplet suspension auxiliary phase flow channel (4) and the solution passed into the shear phase flow channel (3) is greater than the gravity of the droplets.

2. The anti-adhesion microfluidic chip based on the interfacial suspension method according to claim 1, characterized in that: The substrate (1) is provided with a stepped structure (6), specifically: the flow channel height of the inner phase flow channel (2) needs to be higher than the flow channel height of the shear phase flow channel (3), the flow channel height of the shear phase flow channel (3) is higher than the flow channel height of the droplet suspension auxiliary phase flow channel (4), and the flow channel height of the droplet suspension auxiliary phase flow channel (4) is higher than the flow channel height of the droplet transport flow channel (7).

3. The anti-adhesion microfluidic chip based on the interfacial suspension method according to claim 1, characterized in that: The inner phase flow channel (2) is fed with a sodium alginate solution, the shear phase flow channel (3) is fed with liquid paraffin containing Span 80, and the droplet suspension auxiliary phase flow channel (4) is fed with glycerol.

4. The anti-adhesion microfluidic chip based on the interfacial suspension method according to claim 1, characterized in that: Pure water is introduced into the inner phase flow channel (2), dimethyl silicone oil is introduced into the shear phase flow channel (3), and fluorinated oil is introduced into the droplet suspension auxiliary phase flow channel (4).

5. The anti-adhesion microfluidic chip based on the interfacial suspension method according to claim 1, characterized in that: Pure water is introduced into the inner phase flow channel (2), peanut oil is introduced into the shear phase flow channel (3), and dimethyl silicone oil is introduced into the droplet suspension auxiliary phase flow channel (4).

6. The anti-adhesion microfluidic chip based on the interfacial suspension method according to any one of claims 1 to 5, characterized in that: The inner phase solution enters the inner phase flow channel (2) through the inner phase flow channel inlet (9), the shear phase solution enters the shear phase flow channel (3) through the shear phase flow channel inlet (10), an auxiliary phase flow channel rectangular inlet (5) is provided at the intersection of the droplet transport flow channel (7) and the droplet suspension auxiliary phase flow channel (4), the auxiliary phase solution enters the droplet suspension auxiliary phase flow channel (4) through the auxiliary phase flow channel rectangular inlet (5) and the droplet suspension auxiliary phase flow channel circular hole inlets (11) provided at both ends of the droplet suspension auxiliary phase flow channel (4), the inner phase flow channel (2), the auxiliary phase flow channel rectangular inlet (5) and the inlet end of the droplet transport flow channel (7) are connected, the inner phase flow channel inlet (9), the shear phase flow channel inlet (10) and the droplet suspension auxiliary phase flow channel circular hole inlet (11) pass through the upper substrate, and the auxiliary phase flow channel rectangular inlet (5) passes through the upper substrate and the lower substrate.

7. The anti-adhesion microfluidic chip based on the interface suspension method according to any one of claims 1 to 5, characterized in that: The flow cross-section shapes of the inner phase flow channel (2), the shear phase flow channel (3) and the droplet suspension auxiliary phase flow channel (4) are circular or rectangular.

8. The anti-adhesion microfluidic chip based on the interface suspension method according to any one of claims 1 to 5, characterized in that: The upper substrate and the lower substrate are made of transparent material.

9. An anti-adhesion microfluidic chip system based on the interface suspension method according to any one of claims 1 to 8, characterized in that: The inner phase flow channel inlet (9), the shear phase flow channel inlet (10), the droplet suspension auxiliary phase flow channel circular hole inlet (11) and the auxiliary phase flow channel rectangular inlet (5) are respectively connected to a syringe (14) through a hose. The syringe (14) is placed on a syringe pump (13) with adjustable flow rate. The flow channel outlet (8) is connected to a collection beaker (15) through a hose. A microscope imaging device (16) is used to observe the droplet transport flow channel conditions.

10. A method for using the anti-adhesion microfluidic chip based on the interface suspension method according to any one of claims 1 to 8, comprising the following steps: Step 1: introducing a solution that is insoluble in the inner and shear phases and has a large interfacial tension with the shear phase solution into the circular hole inlet (11) of the droplet suspension auxiliary phase flow channel and the rectangular inlet (5) of the auxiliary phase flow channel to fill the droplet transport flow channel (7) in advance; Step 2: injecting a shear phase solution into the inlet of the shear phase flow channel (10), adjusting the flow rate ratio of the shear phase and the auxiliary phase, forming a stable interface channel, and forming a protective layer on the inner wall of the droplet transport flow channel (7); Step 3: Introduce the inner phase solution into the inner phase flow channel (2), adjust the flow rate, and make the inner phase solution be sheared into balls at the intersection of the inner phase flow channel (2) and the shear phase flow channel (3), and flow out along the direction of the droplet transport flow channel (7). Under the influence of gravity, the droplets sink and are finally bound to the interface by the interfacial tension with the auxiliary phase, and are collected after flowing out together with the shear phase and auxiliary phase liquids.

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