Grouting device and grouting method for reinforcing rock fractures through microorganisms

Abstract

The invention discloses a grouting device and method for reinforcing rock fractures through microorganisms, and belongs to the technical field of reinforcing rock fractures through microorganisms. The problems that an existing grouting mold is single in size and inconvenient to use are solved. The device specifically comprises a grouting system, a grouting mold and a recycling mold, grouting liquid is contained in the grouting system; the grouting system is connected with the grouting mold; a rock sample is fixed in the grouting mold; and the rock sample is accommodated in the recovery mold. The grouting system transports slurry into rock sample cracks, and the rock sample cracks are filled and solidified with the slurry; waste liquid flowing out of rock sample cracks after solidification flows into a recovery mold to be collected; according to the grouting method, a bacterial solution and reaction solution separated grouting mode is adopted, and the situation that calcium carbonate deposits in advance, and consequently grouting holes are blocked is avoided; a filter screen is arranged on the grouting outlet plate, so that the retention time of slurry in rock sample cracks is prolonged, and the curing effect is improved; waste liquid after each time of grouting is re-injected into rock sample cracks, so that the utilization rate of the grouting solution is improved.

Description

Technical Field

[0001] The invention relates to the technical field of microbial reinforcement of rock fissures, and in particular to a grouting device and a grouting method for microbial reinforcement of rock fissures. Background Art

[0002] Microbial induced calcium carbonate precipitation (MICP) is a new type of rock fissure reinforcement method, which has the advantages of low carbon, environmental protection, strong environmental adaptability and durability. Its basic principle is to use the metabolic activities of microorganisms to produce urease, which decomposes urea to produce carbonate ions and ammonium ions. The carbonate ions combine with calcium ions in the environment to form stable calcium carbonate crystal precipitation. These calcium carbonates can be filled into rock cracks to form effective cementation, enhancing the mechanical properties and integrity of the rock. The use of traditional cement-based materials for grouting rock cracks usually has the disadvantages of long reinforcement period and poor reinforcement effect of small cracks, while the use of chemical slurries for rock crack grouting is usually costly. Compared with the above methods, MICP technology has stronger fluidity and shorter construction period, and can be quickly injected into the deep cracks for reinforcement. Some researchers have explored the practicality of MICP technology in rock crack reinforcement through indoor experiments.

[0003] However, there are few grouting molds used for the microbial induced calcium carbonate precipitation technology to reinforce rock fissures, and the applicable size is single. Usually, a rock sample of one specification model requires a grouting mold of one size, which is inconvenient to use; and the bacterial liquid and the reaction liquid are mixed in advance, which blocks the grouting hole 211, resulting in uneven reinforcement by the grouting reinforcement method. These shortcomings will lead to artificial disturbances in the transportation and demoulding process, resulting in the occurrence of undesirable phenomena such as rock sample damage, which hinders the development of the application of microbial induced calcium carbonate precipitation technology in rock fissure reinforcement. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a grouting device and a grouting method for reinforcing rock fissures with microorganisms, which solves the problem that the existing grouting molds have a single size and are inconvenient to use.

[0005] In the first aspect, in order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is: A grouting device for reinforcing rock fissures with microorganisms comprises a grouting system, a grouting mould and a recovery mould; the grouting system contains grouting liquid; the grouting system is connected to the grouting mould; a rock sample is fixed in the grouting mould; and the rock sample is accommodated in the recovery mould.

[0006] In this scheme, the grouting system is used to transport the slurry into the rock sample cracks, and the slurry fills and solidifies in the rock sample cracks; after solidification, the waste liquid flowing out of the rock sample cracks flows into the recovery mold for collection. If the bacterial activity in the waste liquid is high, it can be re-injected into the rock sample cracks for further solidification, thereby increasing the utilization rate of the bacterial solution.

[0007] Furthermore, the grouting mold includes a grouting inlet plate, which is provided with a plurality of grouting holes; two fixed plates are respectively provided on both sides of the grouting inlet plate, and the threaded rods at both ends of the grouting inlet plate respectively penetrate the strip holes on the two fixed plates and are connected to nuts; a grouting outlet plate is also provided between the two fixed plates, and a filter screen is provided in the middle of the grouting outlet plate; the threaded rods at both ends of the grouting outlet plate respectively penetrate the strip holes on the two fixed plates and are connected to nuts; the rock sample is clamped between the grouting inlet plate and the grouting outlet plate.

[0008] In this solution, strip holes are provided on the fixed plates on both sides, and the threaded rods at the ends of the grouting inlet plate and the grouting outlet plate can move in the strip holes, which is convenient for adjusting the distance between the grouting inlet plate and the grouting outlet plate and the center of the mold to adapt to rock samples of different sizes. It has strong applicability and a wide range of applications. A filter screen is set on the grouting outlet plate to increase the residence time of the slurry in the cracks and improve the utilization efficiency of the slurry.

[0009] Furthermore, the recycling mold includes a waste box, two support rods are fixed inside the waste box, and two fixed plates are supported on the two support rods; two limit rods are also provided in the waste box, and the two ends of the two limit rods respectively pass through two strip holes on both sides of the waste box and are connected to nuts; the rock sample is confined between the two limit rods.

[0010] In this scheme, the bacterial solution flowing out of the rock sample cracks is recovered into the waste box, and the waste liquid in the waste box can be re-injected into the rock sample cracks for secondary reinforcement, thereby improving the utilization rate of the slurry.

[0011] Furthermore, two limiting grooves are provided on one side of the fixing plate in contact with the two supporting rods, and the two supporting rods are respectively embedded in the two limiting grooves.

[0012] In this solution, the support rods are supported in the limiting grooves, which ensures the stability of the grouting mold and the rock sample during the reinforcement process, improves the reinforcement success rate, and reduces the disturbance to the rock sample.

[0013] Furthermore, a sealing gasket is provided on the inner side of the two fixing plates, one end of the sealing gasket is fixed to the threaded rod at the end of the grouting inlet plate, and the other end of the sealing gasket is fixed to the threaded rod at the end of the grouting outlet plate.

[0014] In this solution, the blocking gasket is used to block the strip holes on the fixed plate to prevent the slurry from flowing out from the side.

[0015] Furthermore, the grouting system includes a bacteria container and a slurry container; the bacteria container and the slurry container are connected to the grouting holes of the grouting inlet plate through a first pipeline and a second pipeline respectively; a first peristaltic pump is provided on the first pipeline, and a second peristaltic pump is provided on the second pipeline.

[0016] In this solution, the bacterial solution and the reaction solution are injected into the rock sample fissures through the first pipeline and the second pipeline respectively, ensuring that the two are mixed and reacted after entering the rock sample fissures.

[0017] Furthermore, one end of the first pipeline connected to the grouting hole is connected to multiple first branch pipes, one end of the second pipeline connected to the grouting hole is connected to multiple second branch pipes, and the multiple first branch pipes and the multiple second branch pipes are alternately connected to several grouting holes.

[0018] In this scheme, the first branch pipe and the second branch pipe are alternately connected to multiple grouting holes, which can maximize the mixing degree of the bacterial solution and the reaction liquid in the rock sample cracks and ensure that the two are fully reacted; the bacterial solution and the reaction liquid are mixed after entering the rock sample cracks to prevent the two from reacting prematurely in the grouting holes and causing blockage of the grouting holes.

[0019] In a second aspect, the present invention provides a grouting method for reinforcing rock fissures with microorganisms based on a grouting device for reinforcing rock fissures with microorganisms provided in the first aspect, comprising the following steps: S1: preparing a bacterial solution and a reaction solution, and transferring the bacterial solution and the reaction solution into a bacterial container and a slurry container respectively; S2: Adjust the width of the rock sample crack and fix the rock sample in the grouting mold; S3: adjusting the pH of the bacterial solution to 6; starting the first peristaltic pump and the second peristaltic pump, injecting the bacterial solution and the reaction solution into the grouting hole through the first pipeline and the second pipeline respectively, to reinforce the cracks in the rock sample; S4: After grouting for 4 hours, the first peristaltic pump and the second peristaltic pump are turned off to detect the bacterial activity in the waste liquid in the waste tank; if the bacterial activity in the waste liquid is higher than 1 mM urea / min, the waste liquid is re-injected into the grouting hole for reinforcement again; if the bacterial activity is lower than 1 mM urea / min, enter S5; S5: Repeat S3-S4, continue to inject the bacterial solution and reaction solution into the cracks of the rock sample until slurry emerges from the grouting hole, and the reinforcement is completed; enter S6; S6: Take the rock sample, soak it in deionized water for 24 hours, and then bake it in an oven at 55°C for 10 hours for curing.

[0020] In this scheme, the bacterial solution and the reaction liquid are injected separately through two pipes to avoid premature mixing and reaction of the two during the grouting process; the pH of the bacterial solution is adjusted before grouting, and the sensitivity of bacteria to the environmental pH can be used to delay the mineralization time of calcium carbonate, making the reinforcement more uniform; after each grouting is completed, the waste liquid is injected into the internal rock sample cracks again according to the bacterial activity in the waste liquid, making full use of the bacterial solution.

[0021] Furthermore, the bacterial solution in S1 was obtained by activating and expanding Bacillus pasteurianus, and the urease activity of the bacterial solution ranged from 9 to 12 mM urea / min, and the concentration range of the bacterial solution was OD 600 =1.2-1.5; the reaction solution is composed of a mixture of equal volumes of 1.5M calcium chloride and 1.5M urea.

[0022] In this scheme, the bacteria has the advantages of high urea hydrolysis efficiency and strong environmental adaptability.

[0023] Furthermore, S2 includes: S201: inserting a prefabricated width limiting sheet through the grouting hole into the crack of the rock sample, and controlling the crack width of the rock sample by the thickness of the limiting sheet; S202: Rotate the nuts at both ends of the grouting inlet plate and the nuts at both ends of the grouting outlet plate so that the rock sample is fixed between the two fixing plates; S303: Finally, pull out the limiting sheet.

[0024] The beneficial effects of the present invention are: In the grouting device for reinforcing rock fissures with microorganisms provided by the present invention, the threaded rods at the ends of the grouting inlet plate and the grouting outlet plate can move along the fixed plates on the left and right sides, and the distance between the front and rear clamps can be controlled by the movement of the threaded rods. It can be used for reinforcing rock samples of various sizes and shapes, meeting the reinforcement of rock samples of different sizes, shapes and crack widths, and has a wide range of applications. In addition, during the demolding process, the design only needs to move the grouting inlet plate and the grouting outlet plate to the side away from the rock sample. This demolding method causes little disturbance to the rock sample, and can avoid damage to the rock sample.

[0025] The grouting method for reinforcing rock fissures with microorganisms provided by the present invention adopts a MICP reinforcement mode of grouting in which a bacterial solution and a reaction solution are separated, thereby avoiding the problem of clogging of grouting holes due to premature deposition of calcium carbonate; a filter screen is arranged on a grouting outlet plate to increase the residence time of the slurry inside the rock sample fissure and improve the curing effect; the waste liquid after each grouting is re-injected into the rock sample fissure, thereby increasing the utilization rate of the grouting solution and reducing the reinforcement cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of a grouting device for reinforcing rock fissures with microorganisms according to the present invention; Figure 2 It is a schematic diagram of the structure of the grouting mold of the present invention; Figure 3 This is a schematic diagram of the recovery mold structure of the present invention; Figure 4 This is a schematic diagram of the assembly structure of the rock sample, grouting mold and recovery mold of the present invention; Figure 5 This is a schematic diagram of the structure of the plugging gasket of the present invention; Figure 6 This is a schematic diagram of the structure of using the second pipeline to recover waste liquid in Example 2.

[0027] Reference numerals: 1. Grouting system; 11. Bacteria container; 12. Slurry container; 13. First pipeline; 14. Second pipeline; 2. Grouting mold; 21. Grouting inlet plate; 211. Grouting hole; 22. Fixing plate; 221. Limiting groove; 23. Grouting outlet plate; 231. Filter screen; 24. Blocking gasket; 3. Recovery mold; 31. Waste box; 32. Support rod; 33. Limiting rod; 4. Rock sample; DETAILED DESCRIPTION The present invention is further described below in conjunction with the accompanying drawings and specific embodiments. The specific implementation of the present invention is described below to facilitate the understanding of the present invention by those skilled in the art, but it should be clear that the present invention is not limited to the scope of the specific implementation. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention defined and determined by the attached claims, these changes are obvious, and all inventions and creations using the concept of the present invention are protected.

[0028] Example 1 like Figure 1 As shown, this embodiment provides a grouting device for reinforcing rock fissures with microorganisms. The grouting mold has a wide range of applications and can be used to reinforce rock samples of various sizes and shapes. It is easy to remove the mold, and the rock sample is slightly disturbed during the demolding process, thereby avoiding damage to the rock sample. It specifically includes: A grouting system 1, a grouting mold 2 and a recovery mold 3; the grouting system 1 is filled with grouting liquid; the grouting system 1 is connected to the grouting mold 2, and a rock sample 4 is fixed in the grouting mold 2; the grouting system 1 is used to transport the slurry to the cracks of the rock sample 4, and the slurry reacts inside the cracks of the rock sample 4 to reinforce the cracks; the rock sample 4 is accommodated in the recovery mold 3, and the reinforced waste liquid flows into the recovery mold 3.

[0029] like Figure 2As shown, the grouting mold 2 includes a grouting inlet plate 21, a grouting outlet plate 23 and two fixed plates 22; a plurality of grouting holes 211 are opened on the grouting inlet plate 21; two fixed plates 22 are respectively arranged on both sides of the grouting inlet plate 21, and the threaded rods at both ends of the grouting inlet plate respectively penetrate the strip holes on the two fixed plates 22 and are connected with nuts; a grouting outlet plate 23 is also arranged between the two fixed plates 22, and a filter screen 231 is arranged in the middle of the grouting outlet plate 23, and the filter screen 231 can increase the residence time of the slurry in the crack and improve the utilization efficiency of the slurry; the threaded rods at both ends of the grouting outlet plate 23 respectively penetrate the strip holes on the two fixed plates 22 and are connected with nuts; the rock sample 4 is clamped between the grouting inlet plate 21 and the grouting outlet plate 23; the threaded rods at the ends of the grouting inlet plate 21 and the grouting outlet plate 23 can move in the strip holes, so as to facilitate the adjustment of the distance between the grouting inlet plate 21 and the grouting outlet plate 23 and the center of the mold, so as to be suitable for rock samples 4 of different sizes.

[0030] In this embodiment, the nuts at both ends of the grouting inlet plate 21 and the grouting outlet plate 23 are preferably butterfly nuts, which are convenient to screw manually.

[0031] like Figure 3 and Figure 4 As shown, the recovery mold 3 includes a waste box 31, a support rod 32 and a limit rod 33; two support rods 32 are fixed inside the waste box 31, and two fixing plates 22 are supported on the two support rods 32; two limit rods 33 are also arranged in the waste box 31, and the two ends of the two limit rods 33 respectively pass through the two strip holes on both sides of the waste box 31 and are connected with nuts; the rock sample 4 is confined between the two limit rods 33; the bacterial solution flowing out of the cracks of the rock sample 4 is recovered into the waste box 31, and the waste liquid in the waste box 31 can be re-injected into the cracks of the rock sample 4 for secondary reinforcement, thereby improving the utilization rate of the slurry.

[0032] Two limiting grooves 221 are provided on one side of the fixing plate 22 that contacts the two supporting rods 32, and the two supporting rods 32 are respectively embedded in the two limiting grooves 221; the supporting rods 32 are supported in the limiting grooves 221, thereby ensuring the stability of the grouting mold 2 and the rock sample 4 during the reinforcement process, improving the reinforcement success rate, and reducing the disturbance to the rock sample 4.

[0033] The inner sides of the two fixing plates 22 are also provided with blocking gaskets 24, and the structure of the blocking gaskets 24 is as follows: Figure 5 As shown; one end of the sealing gasket 24 is fixed to the threaded rod at the end of the grouting inlet plate 21, and the other end of the sealing gasket 24 is fixed to the threaded rod at the end of the grouting outlet plate 23; the sealing gasket 24 is used to seal the strip holes on the fixed plate 22 to prevent the slurry from flowing out from the side.

[0034] The grouting system 1 includes a bacteria container 11, a slurry container 12, a first pipeline 13 and a second pipeline 14; the bacteria container 11 and the slurry container 12 are connected to the grouting hole 211 of the grouting inlet plate 21 through the first pipeline 13 and the second pipeline 14 respectively; a first peristaltic pump is provided on the first pipeline 13, and a second peristaltic pump is provided on the second pipeline 14; the bacterial solution and the reaction liquid are injected into the cracks of the rock sample 4 through the first pipeline 13 and the second pipeline 14 respectively.

[0035] One end of the first pipeline 13 connected to the grouting hole 211 is connected to multiple first branch pipes, and one end of the second pipeline 14 connected to the grouting hole 211 is connected to multiple second branch pipes. The multiple first branch pipes and the multiple second branch pipes are alternately connected to a plurality of grouting holes 211, which can maximize the mixing degree of the bacterial solution and the reaction liquid in the cracks of the rock sample 4 and ensure that the two are fully reacted; the bacterial solution and the reaction liquid are mixed after entering the cracks of the rock sample 4, and the two can also be prevented from reacting prematurely in the grouting hole 211 and causing the grouting hole to be blocked.

[0036] In this embodiment, the first pipeline 13 and the second pipeline 14 are preferably silicone hoses.

[0037] Example 2 like Figure 1-5 As shown, this embodiment provides a grouting method for reinforcing rock fissures with microorganisms based on a grouting device for reinforcing rock fissures provided in Example 1, comprising the following steps: S1: preparing a bacterial solution and a reaction solution, and transferring the bacterial solution and the reaction solution into a bacterial container 11 and a slurry container 12 respectively; The bacterial solution is obtained by activating and expanding Sporosarcina pasteurii, which has the advantages of high urea hydrolysis efficiency and strong environmental adaptability. The urease activity range of the bacterial solution is 9-12mM urea / min, and the concentration range of the bacterial solution is OD600=1.2-1.5. The reaction solution is composed of a mixture of equal volumes of 1.5M calcium chloride and 1.5M urea.

[0038] S2: Adjust the width of the crack of the rock sample 4 and fix the rock sample 4 in the grouting mold 2; specifically including: S201: Pass a prefabricated width limiting sheet through the grouting hole 211 into the crack of the rock sample 4, and control the crack width of the rock sample 4 by the thickness of the limiting sheet; the limiting sheet can be a metal sheet.

[0039] S202: Rotate the nuts at both ends of the grouting inlet plate 21 and the nuts at both ends of the grouting outlet plate 23 so that the rock sample 4 is fixed between the two fixing plates 22; S203: Finally, pull out the limiting sheet.

[0040] S3: Use dilute hydrochloric acid solution to adjust the pH of the bacterial solution to 6; start the first peristaltic pump and the second peristaltic pump to inject the bacterial solution and the reaction solution into the grouting hole 211 through the first pipeline 13 and the second pipeline 14 respectively, so as to reinforce the cracks of the rock sample 4; S4: After grouting for 4 hours, the first peristaltic pump and the second peristaltic pump are turned off to detect the bacterial activity in the waste liquid in the waste tank 31; if the bacterial activity in the waste liquid is higher than 1 mM urea / min, the waste liquid is re-injected into the grouting hole 211 for reinforcement again; if the bacterial activity is lower than 1 mM urea / min, enter S5; When the waste liquid is re-injected into the grouting hole 211, the end of the first pipeline 13 or the second pipeline 14 away from the rock sample 4 can be directly inserted into the waste liquid tank, and then the corresponding peristaltic pump on the pipeline is started to pump the waste liquid into the grouting hole 211, thus saving redundant pipelines. Figure 6 As shown, Figure 6 The end of the second pipeline 14 away from the rock sample 4 is directly inserted into the waste liquid tank.

[0041] S5: Repeat S3-S4, continue to inject the bacterial solution and the reaction solution into the cracks of the rock sample 4, until slurry emerges from the grouting hole 211, and the reinforcement is completed; enter S6; S6: Take rock sample 4, soak it in deionized water for 24 hours, and then bake it in an oven at 55°C for 10 hours for curing; after the curing is completed, carry out subsequent tests according to the test plan.

[0042] In this embodiment, the bacterial solution and the reaction liquid are injected respectively through two pipelines, which can avoid premature mixing and reaction of the two during the grouting process; the pH of the bacterial solution is adjusted before grouting, and the sensitivity of bacteria to the environmental pH can be used to delay the mineralization time of calcium carbonate, making the reinforcement more uniform; after each grouting is completed, the waste liquid is injected into the internal rock sample 4 cracks again according to the bacterial activity in the waste liquid, so as to make full use of the bacterial solution.

[0043] Those skilled in the art will appreciate that the embodiments herein are intended to help readers understand the principles of the present invention, and should be understood that the protection scope of the present invention is not limited to such specific statements and embodiments. Those skilled in the art can make various other specific variations and combinations that do not deviate from the essence of the present invention based on the technical revelations disclosed by the present invention, and these variations and combinations are still within the protection scope of the invention.

Claims

1. A grouting device for reinforcing rock fissures with microorganisms, characterized in that: The invention comprises a grouting system (1), a grouting mould (2) and a recovery mould (3); the grouting system (1) contains grouting liquid; the grouting system (1) is connected to the grouting mould (2); a rock sample (4) is fixed in the grouting mould (2); and the rock sample (4) is accommodated in the recovery mould (3).

2. The grouting device for reinforcing rock fissures by microorganisms according to claim 1, characterized in that: The grouting mold (2) comprises a grouting inlet plate (21), and a plurality of grouting holes (211) are formed on the grouting inlet plate (21); two fixing plates (22) are respectively provided on both sides of the grouting inlet plate (21), and threaded rods at both ends of the grouting inlet plate (21) respectively penetrate the strip holes on the two fixing plates (22) and are connected to nuts; a grouting outlet plate (23) is also provided between the two fixing plates (22), and a filter screen (231) is provided in the middle of the grouting outlet plate (23); threaded rods at both ends of the grouting outlet plate (23) respectively penetrate the strip holes on the two fixing plates (22) and are connected to nuts; The rock sample (4) is clamped between the grouting inlet plate (21) and the grouting outlet plate (23).

3. The grouting device for reinforcing rock fissures by microorganisms according to claim 2 is characterized in that: The recycling mold (3) comprises a waste box (31), two support rods (32) are fixed inside the waste box (31), and the two fixing plates (22) are supported on the two support rods (32); two limiting rods (33) are also arranged inside the waste box (31), and the two ends of the two limiting rods (33) respectively pass through two strip holes on both sides of the waste box (31) and are connected to nuts; the rock sample (4) is limited between the two limiting rods (33).

4. The grouting device for reinforcing rock fissures by microorganisms according to claim 3 is characterized in that: Two limiting grooves (221) are provided on one side of the fixing plate (22) that contacts the two support rods (32), and the two support rods (32) are respectively embedded in the two limiting grooves (221).

5. The grouting device for reinforcing rock fissures by microorganisms according to claim 3, characterized in that: A sealing gasket (24) is also provided on the inner side of the two fixing plates (22), one end of the sealing gasket (24) is fixed to the threaded rod at the end of the grouting inlet plate (21), and the other end of the sealing gasket (24) is fixed to the threaded rod at the end of the grouting outlet plate (23).

6. The grouting device for reinforcing rock fissures by microorganisms according to claim 2, characterized in that: The grouting system (1) comprises a bacteria container (11) and a slurry container (12); the bacteria container (11) and the slurry container (12) are connected to the grouting hole (211) of the grouting inlet plate (21) via a first pipeline (13) and a second pipeline (14), respectively; a first peristaltic pump is provided on the first pipeline (13), and a second peristaltic pump is provided on the second pipeline (14).

7. The grouting device for reinforcing rock fissures by microorganisms according to claim 6, characterized in that: One end of the first pipeline (13) connected to the grouting hole (211) is connected to a plurality of first branch pipes, and one end of the second pipeline (14) connected to the grouting hole (211) is connected to a plurality of second branch pipes, and the plurality of first branch pipes and the plurality of second branch pipes are alternately connected to a plurality of the grouting holes (211).

8. A grouting method using the grouting device for reinforcing rock fissures with microorganisms according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: preparing a bacterial solution and a reaction solution, and transferring the bacterial solution and the reaction solution into a bacterial container (11) and a slurry container (12), respectively; S2: adjusting the width of the crack of the rock sample (4) and fixing the rock sample (4) in the grouting mold (2); S3: adjusting the pH of the bacterial solution to 6; starting the first peristaltic pump and the second peristaltic pump to inject the bacterial solution and the reaction solution into the grouting hole (211) through the first pipeline (13) and the second pipeline (14), respectively, to reinforce the cracks in the rock sample (4); S4: After grouting for 4 hours, the first peristaltic pump and the second peristaltic pump are turned off, and the bacterial activity in the waste liquid in the waste tank (31) is detected; if the bacterial activity in the waste liquid is higher than 1 mM urea / min, the waste liquid is re-injected into the grouting hole (211) for reinforcement again; if the bacterial activity is lower than 1 mM urea / min, the process proceeds to S5; S5: Repeat S3-S4 to continue injecting the bacterial solution and the reaction solution into the cracks of the rock sample (4) until grout emerges from the grouting hole (211), and the reinforcement is completed; Enter S6; S6: Take the rock sample (4), soak it in deionized water for 24 hours, and then bake it in an oven at 55° C. for 10 hours for curing.

9. The grouting method of the grouting device for reinforcing rock fissures with microorganisms according to claim 8, characterized in that: The bacterial solution in S1 is obtained by activating and expanding Bacillus pasteurianus. The urease activity of the bacterial solution is in the range of 9-12 mM urea / min. The concentration range of the bacterial solution is OD 600 =1.2-1.5; the reaction solution is composed of a mixture of equal volumes of 1.5M calcium chloride and 1.5M urea.

10. The grouting method of the grouting device for reinforcing rock fissures with microorganisms according to claim 8, characterized in that: The S2 includes: S201: inserting a prefabricated width limiting sheet through a grouting hole (211) into the crack of the rock sample (4), and controlling the width of the crack of the rock sample (4) by the thickness of the limiting sheet; S202: Rotate the nuts at both ends of the grouting inlet plate (21) and the nuts at both ends of the grouting outlet plate (23) so that the rock sample (4) is fixed between the two fixing plates (22); S303: Finally, pull out the limiting sheet.

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