Method for obtaining sugarcane variety with high smut resistance by using Clerodendron simsii polymer

Abstract

The invention provides a method for obtaining a sugarcane variety with high smut resistance by using a Clerodendron simsii polymer, and relates to the technical field of sugarcane breeding. The method comprises the following steps: hybridizing Yunnan 82-1 (smut resistance) serving as a female parent and Cipmi Yunnan 2017-22 (smut resistance) serving as a male parent to obtain a high-smut-resistance Cipmi hybrid A, hybridizing a high-smut-resistance sugarcane variety CP72-3709 serving as a female parent with A to obtain a hybrid B, hybridizing a high-smut-resistance sugarcane variety CP89-2143 serving as a female parent with the hybrid B to obtain a hybrid C, and hybridizing the hybrid C with Cipmi Yunnan 2017-22 (smut resistance) serving as a female parent with Cipmi Yunnan 2017-22 (smut resistance) serving as a male parent to obtain the high-smut-resistance Cipmi hybrid. And after three times of hybridization, the offspring hybrid C is a high-resistance parent containing two cleft-hand dense consanguinity. According to the method, the hybrid C is hybridized with other high-smut-resistance sugarcane varieties, the high-sugar and high-smut-resistance sugarcane varieties can be obtained, the obtained high-sugar and high-smut-resistance sugarcane varieties are stable in character, and the sugar content and smut resistance of filial generations are remarkably enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of sugarcane breeding, and in particular to a method for obtaining a sugarcane variety with high resistance to smut by using a cutting hand dense polymer. Background Art

[0002] Sugarcane smut is known as the "cancer" of sugarcane, which poses a serious threat to the growth and yield of sugarcane. Sporisorium scitamineum is the pathogen of sugarcane smut, which belongs to the Basidiomycetes. The spores of these fungi can be spread by air currents, rain, irrigation water and insects, fall on sugarcane buds and hide between scales, germinate in water to form infectious hyphae and invade sugarcane buds. The hyphae lurking in the sugarcane buds and the thick-walled spores that fall into the soil become the primary infection source of the next season's sugarcane.

[0003] Existing sugarcane varieties are mainly derived from the hybridization of cultivated original species (tropical species) and wild species (cut-hand dense species, large-stemmed wild species). After years of repeated hybridization, the smut disease was not identified when the varieties were used, and they were directly used, resulting in that almost all existing sugarcane varieties are susceptible to smut disease. Smut disease can cause a reduction in the yield of perennial sugarcane, and even a complete failure in severe cases. Although some methods for preventing and controlling sugarcane smut disease exist, the effect is not ideal, and there are problems such as high cost and complicated operation.

[0004] We have identified more than 300 sugarcane varieties with smut from France, Brazil, the Philippines, the United States, India, Australia, etc. and found that only 11.90% of them are highly resistant, with an average incidence of 12.36-28.26%. Through preliminary experimental research, we found that the incidence of CP72-3709 is 0.00% and the incidence of CP89-2143 is 0.00%. Therefore, if we want to fundamentally solve the problem of smut, we must cultivate sugarcane varieties with high resistance to smut, and obtaining smut-resistant sugarcane varieties must start from the original varieties. Therefore, creating smut-resistant varieties and using disease-resistant varieties to breed high-quality smut-resistant sugarcane varieties is the most effective method, which is of extremely important strategic significance for the prevention and control of sugarcane smut. Summary of the invention

[0005] The object of the present invention is to provide a method for obtaining a sugarcane variety with high resistance to smut by using a cutting hand dense polymer, and the obtained new sugarcane variety has stable high sugar and high resistance to smut traits.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for obtaining a sugarcane variety with high resistance to smut by using a cutting hand dense polymer, characterized in that it comprises the following steps:

[0008] (1) hybridizing a wild smut-resistant variety, Yunnan 82-1, as the female parent and a wild smut-resistant variety, Yunnan 2017-22, as the male parent, cultivating and screening to obtain a highly smut-resistant hybrid variety A;

[0009] (2) using the highly smut-resistant sugarcane variety CP72-3709 as the female parent and hybridizing it with hybrid A, cultivating and screening, and obtaining hybrid B;

[0010] (3) using the highly resistant sugarcane variety CP89-2143 as the female parent to cross with the hybrid B, cultivating and screening to obtain a highly resistant hybrid C containing two parental lines of smut;

[0011] (4) By hybridizing hybrid C with other sugarcane varieties with high resistance to smut, high-sugar and high-smut resistance sugarcane varieties can be obtained.

[0012] Furthermore, the screening criteria in steps (1) to (3) are:

[0013] Resistance Incidence (%) HR (High Resistance) 0.00-6.00 R1(Anti) 6.01-11.00 R2(Anti) 11.01-20.00 MR (Medium Resistance) 20.01-30.00 MS (moderate feeling) 30.01-40.00 S1 (sense) 30.01-40.00 S2(Sense) 40.01-60.00 HS1(High Sensitivity) 60.01-80.00 HS2 (High Sensitivity) 80.01-100.00

[0014] Furthermore, the hybridization steps in steps (1) to (3) are:

[0015] Cultivate parents in barrels, induce flowering of parents under photoperiod conditions, perform combined hybridization of parents, and harvest hybrid seeds;

[0016] Furthermore, the screening method in steps (1) to (3) is:

[0017] The seedling medium is divided into seedling trays, the seeds are scattered with fine sand, sown, watered, and covered with film. After the seedlings grow 3-5 true leaves, they are transplanted and cultured to obtain seedlings. After the seedlings are inoculated, they are transplanted to the field for cultivation.

[0018] Furthermore, the seedling culture medium is prepared according to the ratio of nutrient soil: organic matter: perlite = 3:1.5:0.5, and the culture time is 1 to 2 months.

[0019] Further, the steps of seedling inoculation are:

[0020] A smut spore suspension was prepared according to the germination rate of smut pathogen spores, the roots of the seedlings were immersed in the spore suspension, and after being taken out, they were moisturized with a plastic bag.

[0021] Further, the concentration of the smut spore suspension is 5×10 6 spores / mL, the soaking time is 5 to 20 minutes, the temperature of the plastic bag moisturizing treatment is 25°C to 28°C, and the time is 22 to 26 hours.

[0022] The beneficial effects of the present invention compared with the prior art are:

[0023] The present invention uses wild variety Cut Hand Mi Yunnan 82-1 (smut resistance) as female parent and Cut Hand Mi Yunnan 2017-22 (smut resistance) as male parent to create a high smut resistance Cut Hand Mi hybrid A, then uses high smut resistance sugarcane variety CP72-3709 as female parent to cross with A, obtains hybrid B, then uses high smut resistance sugarcane variety CP89-2143 as female parent to cross with hybrid B, obtains hybrid C, and the offspring hybrid C after three crosses is a high-resistance parent containing two Cut Hand Mi bloodlines. The present invention crosses hybrid C with other high smut resistance and high sugar sugarcane varieties to obtain high sugar and high smut resistance sugarcane varieties, and the obtained high sugar and high smut resistance sugarcane varieties have stable traits, and the sugar content and smut resistance of the hybrid offspring are significantly enhanced. DETAILED DESCRIPTION

[0024] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0025] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0026] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0027] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0028] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0029] The present invention provides a method for obtaining a sugarcane variety with high resistance to smut by using a cutting hand dense polymer, characterized in that it comprises the following steps:

[0030] (1) hybridizing Yunnan 82-1, a variety resistant to smut, as the female parent and Yunnan 2017-22, a variety resistant to smut, as the male parent, cultivating and screening to obtain a hybrid variety A with high resistance to smut;

[0031] (2) using the highly smut-resistant sugarcane variety CP72-3709 as the female parent and hybridizing it with hybrid A, cultivating and screening, and obtaining hybrid B;

[0032] (3) using the highly resistant sugarcane variety CP89-2143 as the female parent to cross with the hybrid B, cultivating and screening to obtain a highly resistant hybrid C containing two parental lines of smut;

[0033] (4) By hybridizing hybrid C with other sugarcane varieties with high resistance to smut, high-sugar and high-smut resistance sugarcane varieties can be obtained.

[0034] The sources and morbidity (natural morbidity) of the materials used in the present invention are shown in Table 1:

[0035] Table 1 Sources of different germplasm resources

[0036] Germplasm source Incidence (%) Yunnan 82-1 National Sugarcane Germplasm Resource Garden 0.00 Yunnan 2017-22 National Sugarcane Germplasm Resource Garden 0.00 CP72-3709 National Sugarcane Germplasm Resource Garden 0.00 CP89-2143 National Sugarcane Germplasm Resource Garden 0.00 Cloud Sugarcane 0551 National Sugarcane Germplasm Resource Garden 2％ Cloud Sugarcane 081608 National Sugarcane Germplasm Resource Garden 25％

[0037] Note: The above-mentioned sugarcane varieties are all from the National Sugarcane Germplasm Resource Garden (Kaiyuan) of the Sugarcane Research Institute of Yunnan Academy of Agricultural Sciences in Yunnan Province. Technical personnel in this field can apply for the above-mentioned sugarcane varieties through the Sugarcane Research Institute of Yunnan Academy of Agricultural Sciences.

[0038] Example 1

[0039] Example 1 of the present invention provides a method for obtaining a sugarcane variety with high resistance to smut by using a cutting hand dense polymer, and the specific steps are as follows:

[0040] (1) The wild smut-resistant variety Yunnan 82-1 (resistant to smut) was used as the female parent and Yunnan 2017-22 (resistant to smut) was used as the male parent. The parents were cultivated by barrel cultivation. Photoperiod flowering induction was started from mid-May to early July. The parents were hybridized and hybrid seeds were harvested. Then, seeding and seedling raising were carried out: the seedling medium was first divided into seedling trays and leveled. The seeds were then scattered with fine sand. The seeds were sown and covered with 1-2 cm of vermiculite. The seeds were watered and covered with film until the seedlings grew 3-5 true leaves and then transplanted. After 2 months of watering and fertilization, the transplanted seedlings were obtained.

[0041] The seedling medium is mixed according to the ratio of nutrient soil: organic matter: perlite = 3:1.5:0.5, and sterilized with a broad-spectrum fungicide at a rate of 15-20g / 50L.

[0042] The transplanted seedlings were inoculated. Before inoculation, the germination rate of smut fungus spores was tested. The smut spore concentration was 5×10 6 Spore suspension of spores / ml. The source of the bacteria is collected in the previous year, dried, sealed in plastic bags, and stored in a low-temperature refrigerator. The roots of the seedlings are immersed in the prepared spore suspension for 10 minutes. After taking out, they are kept moist with plastic bags and placed in a greenhouse at about 25°C for 24 hours to promote spore germination and invasion of root buds. After inoculation, the rows are planted with a row spacing of 1.2 meters and a plant spacing of 50 cm. The row spacing is 6 meters long, and 300 plants are planted with conventional management.

[0043] The survey was repeated three times in different locations. After the seedlings grew up, the basic number of seedlings was investigated. After the tillering was basically completed, the total number of stems was investigated. After the first smut whip grew out, the number of newly grown whips was investigated every 14 days until the end of October. The percentage of the total number of whips to the total number of stem tillers was calculated, and the disease resistance of the test materials was determined by comparing with the grading standards. The hybrid A with high resistance to smut was obtained, and the incidence rate of hybrid A was 3.00%.

[0044] (2) Using CP72-3709 with strong growth vigor, high sugar content and high resistance to smut as the female parent and the hybrid A obtained in step (1) as the male parent, hybridization and high resistance to smut are performed according to the steps of step (1) to obtain hybrid B.

[0045] (3) Using the highly resistant black smut CP89-2143 as the female parent and the hybrid B obtained in step (2) as the male parent, hybridization and high smut resistance screening were performed according to the steps in step (1) to obtain hybrid C (Yunzhe 22-10).

[0046] (4) Using Yunzhe 0551 as the female parent and the hybrid C obtained in step (3) as the male parent, hybridization and screening for high resistance to smut were performed according to the steps in step (1) to obtain hybrid D (Yunzhe 23-1).

[0047] The percentage of the total number of whips of Yunzhe 23-1 to the total number of stem tillers was calculated. The results are shown in the following table:

[0048] Table 2 Survey results of high resistance combination Yunzhe 0551×Yunzhe 22-10

[0049]

[0050] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for obtaining a sugarcane variety with high resistance to smut using a cutter dense polymer, characterized in that: The steps include: (1) hybridizing Yunnan 82-1, a variety resistant to smut, as the female parent and Yunnan 2017-22, a variety resistant to smut, as the male parent, cultivating and screening to obtain a hybrid variety A with high resistance to smut; (2) using the highly smut-resistant sugarcane variety CP72-3709 as the female parent and hybridizing it with hybrid A, cultivating and screening, and obtaining hybrid B; (3) using the highly resistant sugarcane variety CP89-2143 as the female parent to cross with the hybrid B, cultivating and screening to obtain a highly resistant hybrid C containing two parental lines of smut; (4) By hybridizing hybrid C with other sugarcane varieties with high resistance to smut, high-sugar and high-smut resistance sugarcane varieties can be obtained.

2. The method for obtaining a sugarcane variety with high resistance to smut using a cutting hand dense polymer according to claim 1, characterized in that: The screening criteria in steps (1) to (3) are:

3. The method for obtaining a sugarcane variety with high resistance to smut using a cutting hand dense polymer according to claim 1, characterized in that: The hybridization steps in steps (1) to (3) are: The parents are cultivated in barrels, photoperiodic flowering induction is performed on the parents, the parents are combined and hybridized, and hybrid seeds are harvested.

4. The method for obtaining a sugarcane variety with high resistance to smut using a cutting hand dense polymer according to claim 1, characterized in that: The screening method in steps (1) to (3) is: The seedling medium is divided into seedling trays, the seeds are scattered with fine sand, sown, watered, and covered with film. After the seedlings grow 3-5 true leaves, they are transplanted and cultured to obtain seedlings. After the seedlings are inoculated, they are transplanted to the field for cultivation.

5. According to the method for obtaining a sugarcane variety with high resistance to smut using the cut hand dense polymer according to claim 4, the seedling culture medium is prepared according to the ratio of nutrient soil: organic matter: perlite = 3:1.5:0.5, and the culture time is 1 to 2 months.

6. The method for obtaining a sugarcane variety with high resistance to smut using a cutting hand dense polymer according to claim 4, characterized in that: The steps of seedling inoculation are: A smut spore suspension was prepared according to the germination rate of smut pathogen spores, the roots of the seedlings were immersed in the spore suspension, and after being taken out, they were moisturized with a plastic bag.

7. The method for obtaining a sugarcane variety with high resistance to smut using a cutting hand dense polymer according to claim 6, characterized in that: The concentration of the smut spore suspension is 5×10 6 spores / mL, the soaking time is 5 to 20 minutes, the temperature of the plastic bag moisturizing treatment is 25°C to 28°C, and the time is 22 to 26 hours.

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