Siamese bacillus pesticide preparation containing mineral source

Abstract

The invention relates to the technical field of Siamese bacillus pesticide preparations, and discloses a mineral source-containing Siamese bacillus pesticide preparation, which comprises the following ingredients: a main active component, a mineral source component, a synergist, a carrier material, a stabilizer, an antioxidant, a nutritional supplement, a wetting agent, a dispersing agent, an adhesive, a film-forming agent, a pH regulator, a filler and water. According to the siamensis siamensis pesticide preparation containing the mineral source, by using the pH buffering agent and the stabilizing agent, it is ensured that the preparation keeps microbial activity within the wide range of pH 5-9, so that the preparation can adapt to more types of soil including acidic, neutral and alkaline soil, the application range of the preparation is expanded, the preparation is prepared through the freeze-drying technology, and the application range of the preparation is widened. The preparation can effectively protect the activity of microorganisms and reduce damage to cells in the drying process, so that the high survival rate of siamensis amensis in the preparation is ensured, and compared with a traditional preparation, a higher biological control effect is provided.

Description

Technical Field

[0001] The invention relates to the technical field of Siamese bacillus pesticide preparations, in particular to a Siamese bacillus pesticide preparation containing a mineral source. Background Art

[0002] Siamese bacillus pesticide preparation is a biological pesticide that uses Siamese bacillus as an active ingredient. Siamese bacillus is a Gram-positive bacterium that can produce crystalline proteins that are toxic to specific pests. These proteins are highly toxic to many lepidoptera, diptera and coleoptera pests, but are harmless to humans and animals and are environmentally friendly. However, existing Siamese bacillus pesticide preparations have problems with the stability and survival rate of the active ingredients, as well as the dispersion and wettability of the preparations, which lead to the loss of active ingredients during storage and transportation of the preparations, thereby reducing the control effect of the pesticide. When applied, the active ingredients may not reach the expected concentration, affecting the control effect on the target pests. In addition, during the spraying process, the agent cannot be evenly covered on the crop surface, resulting in excessive or low local agent concentrations, which not only affects the control effect, but may also cause drug damage to the crops or cause pests to develop drug resistance. Summary of the invention

[0003] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A Siamese bacillus pesticide preparation containing mineral sources, the ingredients of which include main active ingredients, mineral source ingredients, synergists, carrier materials, stabilizers and antioxidants, nutritional supplements, wetting agents and dispersants, adhesives and film-forming agents, pH regulators, fillers and water.

[0006] As a further solution of the present invention: the ingredients of the Siamese bacillus pesticide preparation are specifically:

[0007] Main active ingredient: Siamese bacillus powder 15%, as an active ingredient for biological control;

[0008] Mineral source ingredients: peat 10%, used to provide necessary mineral elements to support the growth and stability of Siamese bacteria;

[0009] Synergist: 5% glycerin (humectant), helps the preparation stay moist in dry conditions and enhances the survival rate of microorganisms;

[0010] Carrier material: 20% lignite as an organic carrier to help protect the bacteria and provide nutrition;

[0011] Stabilizer and antioxidant: montmorillonite powder 10%, antioxidant (sodium ascorbate) 0.5%, used to protect Siamese bacillus from external environmental influences and increase the storage stability of the preparation.

[0012] As a further solution of the present invention: the Siamese bacillus pesticide formulation ingredients also include:

[0013] Nutritional supplement: Fish meal 5%, providing additional nutrition to promote the growth of Siamese bacillus;

[0014] Wetting agent and dispersant: 10% sorbitol liquid and 5% diatomaceous earth, which help the preparation to disperse better in water and facilitate application;

[0015] Adhesive and film former: 5% xanthan gum, used to improve the adhesion of the preparation and form a protective film on the plant surface;

[0016] pH regulator: 1% sodium carbonate, used to adjust the pH value of the product and improve microbial activity;

[0017] Filler: talc 10%, to ensure the overall volume and weight of the preparation meet the standards;

[0018] Water: Add 100%.

[0019] As a further embodiment of the present invention: the synergist further includes calcium nitrate (1%), the wetting agent and dispersant further include polyethylene glycol (2%), the adhesive and film-forming agent further include an emulsifier such as polyoxyethylene ricinoleate (0.5%), and the filler further includes corn starch (4%), which, as a natural filler, helps to improve the overall texture of the preparation, and is easily degraded under wet conditions to reduce the environmental burden.

[0020] As a further solution of the present invention: the Siamese Bacillus pesticide preparation is prepared by freeze-drying technology, and the process includes bacterial culture, pretreatment, freezing, freeze-drying and packaging.

[0021] As a further solution of the present invention: the freeze-drying technology preparation steps are specifically as follows:

[0022] Bacteria culture: First, under sterile conditions, inoculate Siamese bacteria into a liquid culture medium containing appropriate nutrients and perform fermentation culture until the number of bacteria reaches the required concentration;

[0023] Pretreatment: The cultured bacterial liquid is centrifuged or filtered to separate the bacteria, and then washed to remove residual nutrients and metabolites;

[0024] Freezing: The washed bacteria are suspended in a protective agent (glycerol or trehalose), and then rapidly cooled to a low temperature below -40°C through rapid freezing technology (liquid nitrogen freezing) to form tiny ice crystals and reduce damage to the cell structure;

[0025] Freeze drying: The frozen bacteria are placed in a freeze dryer and sublimated in a vacuum environment. The process converts ice crystals directly into water vapor, thereby removing moisture while maintaining the structure and activity of the bacteria.

[0026] Packaging: The freeze-dried bacterial powder is packaged under sterile conditions, usually in well-sealed aluminum foil bags or glass bottles to prevent moisture and oxidation.

[0027] As a further solution of the present invention: the Rosebacillus pesticide preparation uses a pH buffer and a stabilizer to ensure that the microbial activity is maintained in a wide range of pH 5 to 9.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. By using pH buffers and stabilizers, it is ensured that the preparation maintains microbial activity in a wide range of pH 5 to 9, which makes the preparation adaptable to more types of soils, including acidic, neutral and alkaline soils, thereby expanding its application range;

[0030] 2. Prepared by freeze-drying technology, the preparation of the present invention can effectively protect the activity of microorganisms and reduce damage to cells during the drying process, thereby ensuring a high survival rate of Siamese bacillus in the preparation, and providing a higher biological control effect compared with traditional preparations;

[0031] 3. The preparation of the present invention not only contains the main active ingredients, but also combines a variety of auxiliary ingredients, such as mineral source ingredients, synergists, carrier materials, stabilizers and antioxidants, etc. These ingredients work together to improve the overall performance and stability of the preparation. At the same time, the ingredients used in the preparation of the present invention are mostly natural or easily degradable materials, such as peat soil, lignite, fish meal and corn starch, etc. The ingredients help to reduce the impact on the environment and meet the requirements of sustainable agricultural development. The stabilizers and antioxidants contained in the preparation help to protect Siamese bacillus from the external environment, increase the storage stability of the preparation, and extend the shelf life of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 A comparison chart of application techniques for a Siamese bacillus pesticide preparation containing mineral sources in the prevention and treatment of tomato late blight;

[0033] Figure 2 This is a comparison chart of application techniques for a Siamese bacillus pesticide formulation containing mineral sources in the control of cucumber downy mildew. DETAILED DESCRIPTION

[0034] The present invention will be further described below in conjunction with specific implementation methods, wherein the accompanying drawings are only used for exemplary descriptions and represent only schematic diagrams rather than actual drawings, and should not be understood as limiting the present invention. In order to better illustrate the specific implementation methods of the present invention, some parts of the accompanying drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product.

[0035] Example 1

[0036] See also Figure 1 , which is the first embodiment of the present invention, provides the application of Siamese Bacillus pesticide preparation in the prevention and control of tomato late blight, as follows:

[0037] Pharmaceutical composition:

[0038] Siamese bacillus suspension, 30% content;

[0039] Auxiliary ingredients:

[0040] Gel: 1.5% (sodium carboxymethylcellulose);

[0041] Dispersant: 2.0% (sodium dodecyl sulfate);

[0042] Thickener: 1.0% (xanthan gum);

[0043] Emulsifier: 1.5% (polyoxyethylene sorbitan monooleate);

[0044] Defoaming agent: 0.5% (silicone oil);

[0045] Antifreeze: 1.0% (ethylene glycol);

[0046] Application technique:

[0047] Crop: Tomato;

[0048] Diseases: late blight;

[0049] Dosage: 8-12 ml / mu;

[0050] Application method: spray;

[0051] Timing of application: early stage of disease;

[0052] Application times: Spray 3 times during the growth cycle, with an interval of 7 days between each application;

[0053] Safety interval: 14 days;

[0054] Mass concentration: 300 g / L;

[0055] Specific implementation steps:

[0056] Prepare the medicine:

[0057] Weighing Siamese Bacillus suspension: Using an accurate electronic scale, weigh 300 g Siamese Bacillus suspension;

[0058] Weigh the auxiliary ingredients: Use an accurate electronic scale to weigh the following auxiliary ingredients separately:

[0059] Gel: 15 g (sodium carboxymethyl cellulose);

[0060] Dispersant: 20 g (sodium lauryl sulfate);

[0061] Thickener: 10g (xanthan gum);

[0062] Emulsifier: 15 g (polyoxyethylene sorbitan monooleate);

[0063] Defoaming agent: 5 g (silicone oil);

[0064] Antifreeze: 10 g (ethylene glycol);

[0065] Mix auxiliary ingredients: Place all auxiliary ingredients in a clean mixing container and mix thoroughly using a stirring rod until smooth;

[0066] Add Siamese Bacillus suspension: slowly add the weighed Siamese Bacillus suspension into the mixed auxiliary ingredients, continue stirring to ensure that all ingredients are evenly mixed;

[0067] Add water: slowly add 900 ml of purified water, stirring while adding, until a uniform 300 g / L suspension is formed;

[0068] Field application:

[0069] Calculate the dosage: Calculate the total amount of medicine required according to the field area. If the field area is 1 mu (666.67 square meters), the required dosage is 8-12 ml.

[0070] Filling: Pour the calculated amount of medicine into the medicine box of the sprayer;

[0071] Adjust the sprayer: According to the instructions for use of the sprayer, adjust the spray pressure and nozzle to ensure that the spray is even and fine;

[0072] Application: In the tomato field, spray in an "S" or "Z" shape to ensure that the front and back of each leaf are evenly covered with the pesticide;

[0073] Record the time of application: record the specific date and time of each application, as well as the weather conditions at that time;

[0074] Observation Notes:

[0075] Disease index record: Before applying the pesticide, conduct a disease survey on the tomato plants and record the initial disease index;

[0076] Daily observation: After applying the pesticide, observe the growth of tomato plants at a fixed time every day, including leaf color, plant height, number and size of lesions;

[0077] Disease index update: Update the disease index every day based on observation results and record the development of the lesions;

[0078] Calculation of control effect: After a certain period of time (7 days, 14 days) after application of the pesticide, compare the disease index before and after application and calculate the control effect percentage;

[0079] Data collation: All observation records and calculation results are organized into tables for easy analysis and summary;

[0080] The experimental data are as follows:

[0081]

[0082]

[0083] Calculation formula for prevention effect:

[0084] Conclusion: After the experimental group applied the Siamese bacillus pesticide preparation, the disease index was significantly reduced, and the prevention effect gradually increased with the passage of time. By the 14th day, the prevention effect reached 100%, indicating that the preparation has an excellent prevention and control effect on tomato late blight. Because it is a biological preparation, the Siamese bacillus pesticide preparation has low toxicity to humans and non-target organisms, is safe to use, and does not pose a threat to the health of operators and consumers. By effectively controlling the disease, the preparation helps to increase the yield and quality of tomatoes and increase the economic benefits of farmers.

[0085] Example 2

[0086] See also Figure 2 , which is the second embodiment of the present invention, provides the application of Siamese bacillus pesticide preparation in the prevention and control of cucumber downy mildew, as follows:

[0087] Pharmaceutical composition:

[0088] Siamese bacillus suspension, 30% content;

[0089] Auxiliary ingredients:

[0090] Gel: 1.5% (sodium carboxymethylcellulose);

[0091] Dispersant: 2.0% (sodium dodecyl sulfate);

[0092] Thickener: 1.0% (xanthan gum);

[0093] Emulsifier: 1.5% (polyoxyethylene sorbitan monooleate);

[0094] Defoaming agent: 0.5% (silicone oil);

[0095] Antifreeze: 1.0% (ethylene glycol);

[0096] Application technique:

[0097] Crop: Cucumber;

[0098] Diseases: Downy mildew;

[0099] Dosage: 10-15 ml / mu;

[0100] Application method: spray;

[0101] Timing of application: early stage of disease;

[0102] Application times: Spray twice during the growth cycle, with an interval of 10 days between each application;

[0103] Safety interval: 21 days;

[0104] Mass concentration: 300 g / L;

[0105] Implementation steps:

[0106] Preparation of the agent: The preparation method is the same as that described in Example 1;

[0107] Field application: the method is the same as that described in Example 1;

[0108] Observation record: The method is the same as that described in Example 1;

[0109] The data record table is as follows:

[0110]

[0111] The formula for calculating the protective effect is the same as that described in Example 1;

[0112] in conclusion:

[0113] After the experimental group applied the Siamese bacillus pesticide preparation, the disease index was significantly reduced, and the prevention effect gradually increased with the passage of time. On the 14th day, the prevention effect reached 100%, indicating that the preparation has an excellent prevention and control effect on cucumber downy mildew.

[0114] In summary, by using pH buffers and stabilizers, it is ensured that the preparation maintains microbial activity in a wide range of pH 5 to 9, which enables the preparation to adapt to more types of soils, including acidic, neutral and alkaline soils, thereby expanding its application range. The preparation of the present invention is prepared by freeze-drying technology and can effectively protect the activity of microorganisms and reduce damage to cells during the drying process, thereby ensuring a high survival rate of Siamese bacillus in the preparation, and providing a higher biological control effect compared with traditional preparations. The preparation of the present invention not only contains the main active ingredients, but also combines a variety of auxiliary ingredients, such as mineral source ingredients, synergists, carrier materials, stabilizers and antioxidants, etc. These ingredients work together to improve the overall performance and stability of the preparation. At the same time, the ingredients used in the preparation of the present invention are mostly natural or easily degradable materials, such as peat soil, lignite, fish meal and corn starch, etc. The ingredients help to reduce the impact on the environment and meet the requirements of sustainable agricultural development. The stabilizer and antioxidant contained in the preparation help protect Siamese bacillus from the external environment, increase the storage stability of the preparation, and extend the shelf life of the product.

[0115] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A Siamese bacillus pesticide preparation containing mineral sources, characterized in that: The ingredients include main active ingredients, mineral source ingredients, synergists, carrier materials, stabilizers and antioxidants, nutritional supplements, wetting agents and dispersants, adhesives and film-forming agents, pH regulators, fillers and water.

2. The Siamese Bacillus pesticide preparation containing mineral sources according to claim 1, characterized in that: The ingredients of the Siamese bacillus pesticide preparation are specifically: Main active ingredient: Siamese bacillus powder 15%, as an active ingredient for biological control; Mineral source ingredients: peat 10%, used to provide necessary mineral elements to support the growth and stability of Siamese bacteria; Synergist: 5% glycerin (humectant), helps the preparation stay moist in dry conditions and enhances the survival rate of microorganisms; Carrier material: 20% lignite as an organic carrier to help protect the bacteria and provide nutrition; Stabilizer and antioxidant: montmorillonite powder 10%, antioxidant (sodium ascorbate) 0.5%, used to protect Siamese bacillus from external environmental influences and increase the storage stability of the preparation.

3. The Siamese Bacillus pesticide preparation containing mineral sources according to claim 1, characterized in that: The Siamese bacillus pesticide formulation also includes: Nutritional supplement: Fish meal 5%, providing additional nutrition to promote the growth of Siamese bacillus; Wetting agent and dispersant: 10% sorbitol liquid and 5% diatomaceous earth, which help the preparation to disperse better in water and facilitate application; Adhesive and film former: 5% xanthan gum, used to improve the adhesion of the preparation and form a protective film on the plant surface; pH regulator: 1% sodium carbonate, used to adjust the pH value of the product and improve microbial activity; Filler: talc 10%, to ensure the overall volume and weight of the preparation meet the standards; Water: Add 100%.

4. The Siamese Bacillus pesticide preparation containing mineral sources according to claim 2, characterized in that: The synergist further includes calcium nitrate (1%), the wetting agent and dispersant further includes polyethylene glycol (2%), the binder and film-forming agent further includes an emulsifier such as polyoxyethylene ricinoleate (0.5%), and the filler further includes corn starch (4%), which serves as a natural filler to help improve the overall texture of the preparation and is easily degraded under wet conditions to reduce environmental burden.

5. The Siamese Bacillus pesticide preparation containing mineral sources according to claim 1, characterized in that: The Siamese bacillus pesticide preparation is prepared by freeze-drying technology, and the process includes bacterial culture, pretreatment, freezing, freeze-drying and packaging.

6. The Siamese Bacillus pesticide preparation containing mineral sources according to claim 5, characterized in that: The freeze-drying technology preparation steps are specifically as follows: Bacteria culture: First, under sterile conditions, inoculate Siamese bacteria into a liquid culture medium containing appropriate nutrients and perform fermentation culture until the number of bacteria reaches the required concentration; Pretreatment: The cultured bacterial liquid is centrifuged or filtered to separate the bacteria, and then washed to remove residual nutrients and metabolites; Freezing: The washed bacteria are suspended in a protective agent (glycerol or trehalose), and then rapidly cooled to a low temperature below -40°C through rapid freezing technology (liquid nitrogen freezing) to form tiny ice crystals and reduce damage to the cell structure; Freeze drying: The frozen bacteria are placed in a freeze dryer and sublimated in a vacuum environment. The process converts ice crystals directly into water vapor, thereby removing moisture while maintaining the structure and activity of the bacteria. Packaging: The freeze-dried bacterial powder is packaged under sterile conditions, usually in well-sealed aluminum foil bags or glass bottles to prevent moisture and oxidation.

7. The mineral-source-containing Siamese Bacillus pesticide preparation according to claim 1, characterized in that: The Rosebacillus pesticide formulation uses a pH buffer and a stabilizer to ensure that the microbial activity is maintained in a wide range of pH 5 to 9.

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