Probiotic modified milk powder and preparation method thereof

Abstract

The invention relates to the technical field of food processing, in particular to probiotic modified milk powder and a preparation method thereof. The probiotic modified milk powder is prepared from the following raw materials in parts by weight: 200 to 300 parts of skimmed milk powder, 100 to 200 parts of whole milk powder, 8 to 20 parts of pomegranate extract, 2 to 5 parts of beta-galactosidase, 3 to 5 parts of probiotic powder, 20 to 50 parts of concentrated whey protein powder and 0.5 to 1 part of vitamin. The pomegranate extract is extracted by taking pomegranate seeds as a raw material and water as a solvent. The probiotic modified milk powder provided by the invention can keep the activity of the probiotics for a long time, so that the probiotic modified milk powder can regulate intestinal flora of people with lactose intolerance and promote intestinal absorption. And the probiotics in the probiotic modified milk powder provided by the invention are uniformly mixed, so that the stability of the milk powder is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of food processing, and in particular to a probiotics modulated milk powder and a preparation method thereof. Background Art

[0002] Lactose is mainly found in milk and its products, and needs to be broken down by lactase before it can be absorbed. Lactase deficiency can cause lactose digestion and absorption disorders, and some patients will experience a series of clinical symptoms, mainly abdominal distension, abdominal pain, and diarrhea, which is called lactose intolerance. Lactose intolerance not only affects the absorption of lactose in milk and its products, causing discomfort, but also reduces the absorption and utilization of nutrients such as protein, calcium, and iron in milk and other foods, causing malnutrition.

[0003] Lactase can break down the excess lactose in the human body into glucose and galactose. Lactase can also generate oligosaccharides through transglycosidation in the human body. These oligosaccharides are a kind of low molecular weight, non-viscous water-soluble dietary fiber. They are only used by bifidobacteria in the intestine as a proliferation factor, but not by spoilage bacteria. This can greatly reduce the production of harmful toxins in the intestine and play an important role in preventing constipation and diarrhea. Adding lactase to milk powder can change the problem of lactose intolerance to a certain extent.

[0004] Probiotics are a type of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestines and reproductive system and can produce definite health effects, thereby improving the host's microecological balance and playing a beneficial role. Adding probiotics to milk powder can improve and regulate the flora in the intestines, promote intestinal peristalsis and absorption, and improve lactose intolerance. However, in existing probiotic milk powders, probiotics remain active for a short time and are not suitable for promotion and use. Summary of the invention

[0005] In order to solve the defects of the existing probiotic milk powder in the prior art that the probiotics maintain activity for a short time and are not suitable for popularization and use, the present invention provides a probiotic formula milk powder and a preparation method thereof.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions.

[0007] The invention provides a probiotics modulated milk powder, which is composed of the following raw materials in parts by weight: 200-300 parts of skim milk powder, 100-200 parts of whole milk powder, 8-20 parts of pomegranate extract, 2-5 parts of beta-galactosidase, 3-5 parts of probiotic powder, 20-50 parts of concentrated whey protein powder, 0.5-1 part of beta-carotene, and 0.5-1 part of vitamins;

[0008] Pomegranate extract is obtained by extracting pomegranate seeds as raw material and using water as solvent.

[0009] In some embodiments of the present invention, the pomegranate extract is prepared by mixing pomegranate seeds with water, extracting at 35-70° C. for 60-90 min, collecting the filtrate, and vacuum freeze-drying the filtrate to obtain the pomegranate extract.

[0010] In some embodiments of the present invention, the mass volume ratio of the pomegranate seeds to water is 1-5 g:20-80 mL.

[0011] In some embodiments of the present invention, the number of extractions is 1 to 3 times.

[0012] In some embodiments of the present invention, the pomegranate seeds are crushed and then mixed with water.

[0013] In some embodiments of the present invention, the probiotic powder is two or more of Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus mucosa fermentation, Bifidobacterium breve, and Lactobacillus bulgaricus.

[0014] In some embodiments of the present invention, in the probiotic powder, the mass percentage of animal Bifidobacterium is 2% to 5%, the mass percentage of Bifidobacterium lactis is 4% to 6%, the mass percentage of Lactobacillus rhamnosus is 2% to 3%, the mass percentage of Lactobacillus reuteri is 1% to 2%, the mass percentage of fermented mucus Lactobacillus is 2% to 3%, the mass percentage of Bifidobacterium breve is 1.5 to 2%, and the mass percentage of Lactobacillus bulgaricus is 2% to 8%.

[0015] The number of live bacteria in the bacterial powders of animal bifidobacterium, lactis bifidobacterium, rhamnosus lactobacillus, reuteri lactobacillus, fermented mucus lactobacillus, breve bifidobacterium and bulgaricus lactobacillus is not less than 3.0×10 10 cfu / g.

[0016] In some embodiments of the present invention, the vitamins include one or more of vitamin A, vitamin D, vitamin E, vitamin B1, vitamin B6, folic acid, niacin and pantothenic acid.

[0017] The present invention also provides a method for preparing the probiotics modulated milk powder, comprising the following steps:

[0018] The skimmed milk powder, whole milk powder, pomegranate extract, β-galactosidase, probiotic powder, concentrated whey protein powder, β-carotene and vitamins are dry-mixed uniformly according to proportions, and the mixture is sieved to obtain the probiotics-modulated milk powder.

[0019] In some embodiments of the present invention, the rotation speed of the dry mixing is 1500-2500 r / min, and the dry mixing time is 20-35 min.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The probiotic formula milk powder provided by the present invention is added with pomegranate extract. With the combination of pomegranate extract and multiple ingredients, the probiotics can maintain activity for a long time, inhibit the reproduction of harmful bacteria in the intestine, reduce toxins, promote intestinal peristalsis, thereby improving intestinal function and defecation conditions, which is beneficial to maintaining the nutritional components of the milk powder and effectively improving the problem of lactose intolerance.

[0022] The probiotics-modulated milk powder provided by the present invention contains pomegranate seed extract, which can well maintain the lactase activity in the milk powder. In addition, the pomegranate seed extract can provide the milk powder with a unique taste and smell, making it more popular with consumers. DETAILED DESCRIPTION

[0023] The present invention is described in detail below in conjunction with specific examples, but it should not be construed as limiting the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0024] 1. Experimental materials:

[0025] β-Galactosidase was purchased from Shandong Yiwei Bioengineering Co., Ltd.

[0026] Bifidobacterium animalis, Bifidobacterium lactis, and Lactobacillus bulgaricus were purchased from Inner Mongolia Puze Biological Products Co., Ltd.; Lactobacillus rhamnosus strain was purchased from Inner Mongolia Puze Biological Products Co., Ltd., strain number: CICC 6137; Lactobacillus reuteri, strain number: CICC 6119, Lactobacillus fermentum, strain number: CICC 6233, and Bifidobacterium breve were purchased from China Industrial Microbiological Culture Collection Center, strain number: CICC 6181.

[0027] Concentrated whey protein powder was purchased from Valio (Shanghai) Food Trading Co., Ltd.; β-carotene was purchased from Shandong Yunzhiran Biotechnology Co., Ltd.

[0028] Lactose is mainly found in milk and its products, and needs to be broken down by lactase before it can be absorbed. Lactase deficiency can cause lactose digestion and absorption disorders. Some patients will experience a series of clinical symptoms, mainly abdominal distension, abdominal pain, and diarrhea, which is called lactose intolerance. Lactase can break down excess lactose in the human body into glucose and galactose. Lactase can also generate oligosaccharides in the human body through transglycosidation. These oligosaccharides are a low molecular weight, non-viscous water-soluble dietary fiber. It is only used by bifidobacteria in the intestine as a proliferation factor, but not by spoilage bacteria. This can greatly reduce the production of harmful toxins in the intestine and plays an important role in preventing constipation and diarrhea. Adding lactase to milk powder can change the problem of lactose intolerance to a certain extent. Probiotics are a type of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize in the human intestine and reproductive system, can produce definite health effects, thereby improving the host's microecological balance and playing a beneficial role. Adding probiotics to milk powder can improve and regulate the flora in the intestine, promote intestinal peristalsis and absorption, and improve the problem of lactose intolerance. However, the probiotics in existing probiotic milk powders remain active for a short time and are not suitable for promotion and use.

[0029] In order to solve the defect that the probiotics in the existing probiotic milk powder in the prior art have a short active time and are not suitable for promotion and use, the present invention provides a probiotics modulated milk powder, which is composed of the following raw materials in parts by weight: 200-300 parts of skim milk powder, 100-200 parts of whole milk powder, 8-20 parts of pomegranate extract, 2-5 parts of β-galactosidase, 3-5 parts of probiotic powder, 20-50 parts of concentrated whey protein powder, 0.5-1 part of β-carotene, and 0.5-1 part of vitamins; pomegranate extract is obtained by extracting pomegranate seeds as raw materials and water as solvent. The probiotics modulated milk powder provided by the present invention is added with pomegranate extract. With the cooperation of pomegranate extract and multiple ingredients, the probiotics can maintain the activity of production time, inhibit the reproduction of harmful bacteria in the intestine, reduce toxins, promote intestinal peristalsis, thereby improving intestinal function, improving defecation conditions, and is conducive to maintaining the nutritional components of milk powder and effectively improving the problem of lactose intolerance. In addition, pomegranate seed extract can well maintain the lactase activity in milk powder. And pomegranate seed extract can provide milk powder with a unique taste and smell, making it more popular with consumers.

[0030] Example 1: A probiotics formula milk powder

[0031] A probiotics modulated milk powder is composed of the following raw materials in parts by weight: 200g skim milk powder, 100g whole milk powder, 8g pomegranate extract, 2g beta-galactosidase, 3g probiotic powder, 20g concentrated whey protein powder, 0.5g beta-carotene and 0.5g vitamins.

[0032] In the probiotic powder, the mass percentage of animal bifidobacterium is 2%, the mass percentage of lactobacillus is 4%, the mass percentage of rhamnosus lactobacillus is 2%, the mass percentage of Lactobacillus reuteri is 1%, the mass percentage of fermented mucus lactobacillus is 2%, the mass percentage of Bifidobacterium breve is 1.5%, and the mass percentage of Lactobacillus bulgaricus is 2%. In this embodiment, the number of viable cells contained in the bacterial powders of animal bifidobacterium, bifidobacterium lactis, rhamnosus lactobacillus, Lactobacillus reuteri, fermented mucus lactobacillus, Bifidobacterium breve, and Lactobacillus bulgaricus is not less than 3.0×10 10 cfu / g.

[0033] Preparation method of pomegranate extract: After crushing the pomegranate seeds, mix them with water at a mass volume ratio of 1g:20mL, then extract at 60°C for 70min, extract twice, collect the filtrates twice, combine the filtrates and perform vacuum freeze-drying to obtain a solid that is the pomegranate extract.

[0034] The preparation method of probiotics modulated milk powder is as follows: dry-mix the skim milk powder, whole milk powder, pomegranate extract, β-galactosidase, probiotic powder, concentrated whey protein powder, β-carotene and vitamins in proportion at 1500r / min for 30min, and obtain the probiotics modulated milk powder after sieving.

[0035] Embodiment 2:

[0036] A probiotics-modulated milk powder is composed of the following raw materials in parts by weight: 300 g of skim milk powder, 200 g of whole milk powder, 20 g of pomegranate extract, 5 g of beta-galactosidase, 5 g of probiotic powder, 50 g of concentrated whey protein powder, 1 g of beta-carotene and 1 g of vitamins.

[0037] In the probiotic powder, the mass percentage of animal bifidobacterium is 5%, the mass percentage of lactobacillus is 6%, the mass percentage of rhamnosus lactobacillus is 3%, the mass percentage of Lactobacillus reuteri is 2%, the mass percentage of fermented mucus lactobacillus is 3%, the mass percentage of Bifidobacterium breve is 2%, and the mass percentage of Lactobacillus bulgaricus is 8%. In this embodiment, the number of viable cells contained in the bacterial powders of animal bifidobacterium, bifidobacterium lactis, rhamnosus lactobacillus, Lactobacillus reuteri, fermented mucus lactobacillus, Bifidobacterium breve, and Lactobacillus bulgaricus is not less than 3.0×10 10 cfu / g.

[0038] Preparation method of pomegranate extract: After crushing the pomegranate seeds, mix them with water at a mass volume ratio of 5g:800mL, then extract at 40°C for 90min, extract twice, collect the filtrates twice, combine the filtrates and perform vacuum freeze-drying to obtain a solid that is the pomegranate extract.

[0039] The preparation method of probiotics modulated milk powder is as follows: dry-mix the skim milk powder, whole milk powder, pomegranate extract, β-galactosidase, probiotic powder, concentrated whey protein powder, β-carotene and vitamins in proportion at 2000 r / min for 30 minutes, and obtain the probiotics modulated milk powder after sieving.

[0040] Embodiment 3:

[0041] A probiotics modulated milk powder is composed of the following raw materials in parts by weight: 250g skim milk powder, 150g whole milk powder, 15g pomegranate extract, 3g beta-galactosidase, 4g probiotic powder, 25g concentrated whey protein powder, 0.5g beta-carotene and 0.5g vitamins.

[0042] In the probiotic powder, the mass percentage of animal bifidobacterium is 4%, the mass percentage of lactobacillus is 5%, the mass percentage of rhamnosus lactobacillus is 3%, the mass percentage of Lactobacillus reuteri is 2%, the mass percentage of fermented mucus lactobacillus is 2%, the mass percentage of Bifidobacterium breve is 2%, and the mass percentage of Lactobacillus bulgaricus is 6%. In this embodiment, the number of viable cells contained in the bacterial powders of animal bifidobacterium, bifidobacterium lactis, rhamnosus lactobacillus, Lactobacillus reuteri, fermented mucus lactobacillus, Bifidobacterium breve, and Lactobacillus bulgaricus is not less than 3.0×10 10 cfu / g.

[0043] Preparation method of pomegranate extract: After crushing the pomegranate seeds, mix them with water at a mass volume ratio of 3g:40mL, then extract at 70°C for 90min, extract twice, collect the filtrates twice, combine the filtrates and perform vacuum freeze-drying to obtain a solid that is the pomegranate extract.

[0044] The preparation method of probiotics modulated milk powder is as follows: dry-mix the skim milk powder, whole milk powder, pomegranate extract, β-galactosidase, probiotic powder, concentrated whey protein powder, β-carotene and vitamins in proportion at 2500r / min for 30min, and obtain the probiotics modulated milk powder after sieving.

[0045] Comparative Example 1:

[0046] Compared with the embodiment, the probiotics formulated milk powder does not contain pomegranate extract.

[0047] A probiotics modulated milk powder is composed of the following raw materials in parts by weight: 200g skim milk powder, 100g whole milk powder, 2g beta-galactosidase, 3g probiotic powder, 20g concentrated whey protein powder, 0.5g beta-carotene and 0.5g vitamins.

[0048] In the probiotic powder, the mass percentage of animal bifidobacterium is 2%, the mass percentage of lactobacillus is 4%, the mass percentage of rhamnosus lactobacillus is 2%, the mass percentage of Lactobacillus reuteri is 1%, the mass percentage of fermented mucus lactobacillus is 2%, the mass percentage of Bifidobacterium breve is 1.5%, and the mass percentage of Lactobacillus bulgaricus is 2%. In this embodiment, the number of viable cells contained in the bacterial powders of animal bifidobacterium, bifidobacterium lactis, rhamnosus lactobacillus, Lactobacillus reuteri, fermented mucus lactobacillus, Bifidobacterium breve, and Lactobacillus bulgaricus is not less than 3.0×10 10 cfu / g.

[0049] The preparation method of probiotics modulated milk powder is as follows: dry-mix the skim milk powder, whole milk powder, pomegranate extract, β-galactosidase, probiotic powder, concentrated whey protein powder, β-carotene and vitamins in proportion at 1500r / min for 30min, and obtain the probiotics modulated milk powder after sieving.

[0050] Probiotic activity detection: The number of viable probiotic bacteria in the probiotic modulated milk powder provided by Examples 1 to 3 and Comparative Example 1 was measured after 0 days, 10 days and 25 days respectively. Storage conditions: 10 to 25° C., protected from light.

[0051] The measurement results are shown in Table 1. The probiotics in the probiotic formula milk powder provided by Examples 1 to 3 of the present invention can still maintain a high activity after being stored for 25 days.

[0052] Table 1 Probiotic viable count (CFU / g)

[0053] Group 0d 10d 25d Example 1 <![CDATA[7.9×10 9 ]]> <![CDATA[6.8×10 9 ]]> <![CDATA[5.3×10 9 ]]> Example 2 <![CDATA[7.7×10 9 ]]> <![CDATA[6.6×10 9 ]]> <![CDATA[5.1×10 9 ]]> Example 3 <![CDATA[7.8×10 9 ]]> <![CDATA[6.1×10 9 ]]> <![CDATA[5.2×10 9 <!-- 4 -->]]> Comparative Example 1 <![CDATA[7.7×10 9 ]]> <![CDATA[5.3×10 9 ]]> <![CDATA[4.0×10 9 ]]>

[0054] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0055] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A probiotics formulated milk powder, characterized in that: The invention is composed of the following raw materials in parts by weight: 200-300 parts of skim milk powder, 100-200 parts of whole milk powder, 8-20 parts of pomegranate extract, 2-5 parts of beta-galactosidase, 3-5 parts of probiotic powder, 20-50 parts of concentrated whey protein powder, 0.5-1 part of beta-carotene and 0.5-1 part of vitamins; Pomegranate extract is obtained by extracting pomegranate seeds as raw material and using water as solvent.

2. The probiotics formula milk powder according to claim 1, characterized in that: The preparation method of pomegranate extract is as follows: pomegranate seeds are mixed with water, extracted at 35-70° C. for 60-90 minutes, filtrate is collected, and the filtrate is vacuum freeze-dried to obtain pomegranate extract.

3. The probiotics formula milk powder according to claim 2, characterized in that: The mass volume ratio of the pomegranate seeds to water is 1-5 g:20-80 mL.

4. The probiotics formula milk powder according to claim 2, characterized in that: The number of extractions is 1 to 3 times.

5. The probiotics formulated milk powder according to claim 2, characterized in that: The pomegranate seeds are crushed and then mixed with water.

6. The probiotics formula milk powder according to claim 1, characterized in that: The probiotic powder is two or more of Bifidobacterium animalis, Bifidobacterium lactis, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus mucosa fermentation, Bifidobacterium breve and Lactobacillus bulgaricus.

7. The probiotics formulated milk powder according to claim 6, characterized in that: In the probiotic powder, the mass percentage of animal bifidobacterium is 2% to 5%, the mass percentage of lactic acid bifidobacterium is 4% to 6%, the mass percentage of rhamnosus lactobacillus is 2% to 3%, the mass percentage of reuteri lactobacillus is 1% to 2%, the mass percentage of fermented mucus lactobacillus is 2% to 3%, the mass percentage of breve bifidobacterium is 1.5 to 2%, and the mass percentage of bulgaricus lactobacillus is 2% to 8%.

8. The probiotics formulated milk powder according to claim 1, characterized in that: The vitamins include one or more of vitamin A, vitamin D, vitamin E, vitamin B1, vitamin B6, folic acid, niacin and pantothenic acid.

9. The method for preparing the probiotics modulated milk powder according to claim 1, characterized in that: The following steps are involved: The skimmed milk powder, whole milk powder, pomegranate extract, β-galactosidase, probiotic powder, concentrated whey protein powder, β-carotene and vitamins are dry-mixed uniformly according to proportions, and the mixture is sieved to obtain the probiotics-modulated milk powder.

10. The preparation method according to claim 9, characterized in that: The rotation speed of the dry mixing is 1500-2500 r / min, and the dry mixing time is 20-35 min.