Additive for improving egg shell quality of raised chickens by using 25-OH-VD3 and preparation method of additive

Abstract

The invention relates to the technical field of laying hen breeding, and discloses an additive for improving the egg shell quality of bred chickens by using 25-OH-VD3 and a preparation method thereof, the additive mainly comprises independently researched and developed 25-OH-VD3, 14 full-nutrient vitamins, preferably multiple organic trace elements, a special synbiotics probiotic factor combination bag and the like. Aiming at the main reason of egg shell quality degradation in the production of stacked high-density caged laying hens, the additive of the laying hen feed is developed, and the bone calcium turnover and egg shell forming ability of the laying hens is enhanced; the requirements of high-quality formation of eggshells or substances for regulating and controlling key metabolism are met; the key health guarantee of the laying hens produced by high-quality eggshells is enhanced, and the laying hens can continuously produce the eggs with the high-quality eggshells through the unique nutrition balancing and efficient immunization effects of the additive.

Description

Technical Field

[0001] The invention relates to the technical field of laying hen breeding, and in particular to an additive for improving egg shell quality by utilizing 25-OH-VD3 and a preparation method thereof. Background Art

[0002] In recent years, laying hen farming in my country is undergoing a profound transformation and upgrading. Modern intensive farming, represented by large-scale, high-density, stacked three-dimensional cage farming, has developed rapidly and has gradually become the main model of laying hen farming in my country, greatly increasing the egg production of laying hens. However, the eggshell quality of laying hens raised using this model is generally poor in the later stages of laying, with an egg breakage rate of 7% to 10%.

[0003] The reasons for poor eggshell quality in laying hens in high-density farming models in the later stage of egg production are multi-source, and mainly include the following three aspects: First, the problem of eggshell formation ability, including bone calcium turnover and eggshell formation in the oviduct; second, the degree to which the materials required for eggshell formation are met, mainly including the level and quality of feed and body metabolism supply; third, the problem of laying hen health protection, mainly including the health of the intestines, liver, and reproductive systems related to eggshell formation, multi-source stress response in high-density farming, and antioxidant prevention of functional aging of chickens. These three major sources of "three" systemic causes work together to merge into "one", highlighting the "three-in-one" comprehensive negative effects of abnormal eggshell quality.

[0004] Currently, the existing related patented technologies, research and development and application technologies are not the foundation of systematic technical response solutions from the source, so their application effects have certain limitations. The main characteristics are: First, the use of normal eggshell quality improvement technology to deal with new problems under the new breeding model is ineffective, because the deterioration of eggshell quality is highly correlated with the new breeding model; Second, there are few initiatives that seek systematic breakthroughs based on the excavation of the "three-in-one" systemic causes, and they are piecemeal and have poor overall effects; Third, more attention is paid to production indicators, while less attention is paid to nutritional and immune measures for laying hens related to production. The health of laying hens is difficult to guarantee, and the health problems of laying hens cannot be solved, so it is difficult to improve the eggshell quality.

[0005] 25-OH-VD3 (25-OH-VD3) is vitamin D3 activated by the liver in animals, which can achieve all-round regulation of the animal immune system. Especially with the rapid expansion of intensive and high-density livestock and poultry farming in recent years, the metabolic pressure on livestock and poultry livers has become increasingly greater, and liver syndrome has become increasingly complex, resulting in vitamin D not being effectively activated through the liver. Its extensive metabolism and immune regulation are severely blocked, so the application of 25-OH-VD3 has received more and more attention from the industry.

[0006] Based on the above-mentioned unique and extensive comprehensive effects of 25-OH-VD3 in regulating metabolism, immunity, strengthening bones, etc., combined with the fact that modern laying hen production must use a systematic approach to solve the systematic problem of eggshell quality, in order to make up for the shortcomings of the piecemeal and non-systematic methods of the prior art, the present invention uses the high-quality 25-OH-VD3 independently developed by the unit as the main active substance, and the application of nutritional and immune feed additives as a breakthrough, to develop an efficient, systematic technology dedicated to improving the eggshell quality of modern large-scale high-density laying hen farming, and to innovate a new path for modern laying hen farming science and technology.

[0007] At present, the existing technology adopts normal eggshell quality improvement technology, which is difficult to cope with new problems under the new breeding model. It pays little attention to the nutritional immunity measures of laying hens, making it difficult to ensure the health of laying hens, which makes it difficult to effectively improve the eggshell quality. Therefore, it does not meet the existing needs. In this regard, we propose an additive that uses 25-OH-VD3 to improve the eggshell quality of farmed eggs and a preparation method thereof. Summary of the invention

[0008] The present invention provides an additive for improving egg shell quality by utilizing 25-OH-VD3 and a preparation method thereof, which has the beneficial effect of ensuring the health of laying hens as much as possible, thereby effectively improving the egg shell quality, and solves the problem mentioned in the above background technology that the prior art adopts normal egg shell quality improvement technology, is difficult to cope with new problems under the new breeding model, pays little attention to the nutritional immunity measures of laying hens, makes it difficult to ensure the health of laying hens, and thus leads to the problem that it is difficult to effectively improve the egg shell quality.

[0009] The present invention provides the following technical solution: a method for preparing an additive for improving the quality of chicken egg shells by using 25-OH-VD3, comprising the following specific steps:

[0010] S1. Prepare the following raw materials by weight: 0.10-0.40 parts of vitamin A; 0.10-0.40 parts of α-tocopheryl acetate; 2-6 parts of ascorbic acid; 0.03-0.09 parts of sodium bisulfite menadione; 0.01-0.04 parts of thiamine; 0.20-0.50 parts of niacin; 0.01-0.06 parts of biotin; 0.02-0.08 parts of cobalamin; 0.01-0.02 parts of folic acid; 0.05-0.13 parts of calcium pantothenate; 0.02-0.07 parts of pyridoxine; 0.10-0.20 parts of riboflavin; 0.01-0.03 parts of inositol; 25-OH-VD3 0.10-0.20 parts; choline chloride 1-4 parts; synbiotic prebiotic compound factor 9-19 parts, methionine copper 0.54-0.86 parts; glycine iron 2.49-3.81 parts; methionine zinc 2.46-3.77 parts; methionine manganese 4.03-6.14 parts; calcium hydrogen phosphate 20-45 parts; zeolite powder 8-12 parts; rice husk powder 7.12-43.20 parts;

[0011] S2. The above raw material components are stirred and mixed to prepare an additive;

[0012] The contents of the 9-19 parts of synbiotic prebiotic compound factors are: 1.5-3 parts of Bacillus licheniformis; 2-4 parts of Bacillus subtilis; 1-4 parts of oligofructose; 1-4 parts of chitosan oligosaccharide; 4-6 parts of lactic acid bacteria metabolites; and 1-3 parts of synergistic factor yucca extract.

[0013] The content of active substances is as follows: vitamin A is 500,000 IU / g; α-tocopheryl acetate is 50%; sodium menadione bisulfite is ≥50%; thiamine nitrate is ≥98.0%; niacin is ≥99.0%; biotin is ≥2.0%; 25-OH-VD3 is 0.05%; cyanocobalamin is ≥1.0%; folic acid is ≥95.0%; calcium pantothenate is ≥98.0%; pyridoxine hydrochloride is ≥98.0%; riboflavin is ≥80%; choline chloride is ≥60%; ascorbic acid is ≥99.0%; Bacillus licheniformis is ≥1.2×109cfu / g; Bacillus subtilis is ≥2.2×1010cfu / g; copper methionine is ≥17%; iron glycine is ≥17%; zinc methionine is ≥17.2%; manganese methionine is ≥14.5%.

[0014] The 25-OH-VD3 main effect substance is a unique innovative synthetic product of the inventor's company. The preparation method thereof is as follows: dissolving 25-hydroxy-7-dehydrocholesterol in a mixed solvent system comprising a polar solvent and a non-polar solvent, wherein the volume ratio of the polar solvent to the non-polar solvent is 9-4:1, adding an antioxidant, and reacting under light; monitoring the reaction process with high performance liquid chromatography, controlling the raw material conversion rate to be 32-39%, separating and recovering 25-hydroxy-7-dechlorocholesterol after the reaction, and then heating the remaining reactant after separating 25-hydroxy-7-dehydrocholesterol for isomerization, and then freezing for recrystallization to obtain the 25-OH-VD3 crystals, wherein the purity of the 25-OH-VD3 crystals is ≥94%.

[0015] As an optional solution of the method for preparing an additive for improving the quality of chicken egg shells by using 25-OH-VD3 according to the present invention, the method comprises the following specific steps:

[0016] S1. Prepare the following raw materials by weight: 0.03 parts of vitamin A; 0.02 parts of Dl-α-tocopheryl acetate; 4 parts of L-ascorbic acid; 0.05 parts of sodium bisulfite menadione; 0.03 parts of thiamine; 0.35 parts of niacin; 0.04 parts of biotin; 0.05 parts of cobalamin; 0.01 parts of folic acid; 0.08 parts of calcium pantothenate; 0.04 parts of pyridoxine; 0.15 parts of riboflavin; 0.02 parts of inositol; 0.15 parts of 25-hydroxy VD3; 2.5 parts of choline chloride; 18 parts of synbiotic prebiotic complex factors; 0.65 parts of copper methionine; 3.24 parts of glycine iron; 3.25 parts of methionine zinc; 5.25 parts of methionine manganese; 35 parts of calcium hydrogen phosphate; 12 parts of zeolite powder; 15.09 parts of rice husk powder;

[0017] S2. The above raw material components are stirred and mixed to prepare an additive;

[0018] The synbiotic prebiotic compound factor 18 parts contains: 2.5 parts of Bacillus licheniformis; 3 parts of Bacillus subtilis; 2.5 parts of oligofructose, 2.5 parts of chitosan oligosaccharide, 5 parts of lactic acid bacteria metabolites, and 2.5 parts of synergistic factor yucca extract.

[0019] The content of active substances is as follows: vitamin A is 500,000 IU / g; α-tocopheryl acetate is 50%; sodium menadione bisulfite is ≥50%; thiamine nitrate is ≥98.0%; niacin is ≥99.0%; biotin is ≥2.0%; 25-OH-VD3 is 0.05%; cyanocobalamin is ≥1.0%; folic acid is ≥95.0%; calcium pantothenate is ≥98.0%; pyridoxine hydrochloride is ≥98.0%; riboflavin is ≥80%; choline chloride is ≥60%; ascorbic acid is ≥99.0%; Bacillus licheniformis is ≥1.2×109cfu / g; Bacillus subtilis is ≥2.2×1010cfu / g; copper methionine is ≥17%; iron glycine is ≥17%; zinc methionine is ≥17.2%; manganese methionine is ≥14.5%.

[0020] The 25-OH-VD3 main effect substance is a unique innovative synthetic product of the inventor's company. The preparation method thereof is as follows: dissolving 25-hydroxy-7-dehydrocholesterol in a mixed solvent system comprising a polar solvent and a non-polar solvent, wherein the volume ratio of the polar solvent to the non-polar solvent is 9-4:1, adding an antioxidant, and reacting under light; monitoring the reaction process with high performance liquid chromatography, controlling the raw material conversion rate to be 32-39%, separating and recovering 25-hydroxy-7-dechlorocholesterol after the reaction, and then heating the remaining reactants after separating 25-hydroxy-7-dehydrocholesterol for isomerization, and then freezing for recrystallization to obtain the 25-OH-VD3 crystals, wherein the purity of the 25-OH-VD3 crystals is ≥94%.

[0021] An additive for improving the egg shell quality of chicken eggs by using 25-OH-VD3, wherein: 2 kg of the additive is added per ton of compound feed, and the additive can be increased by 10%-30% in the feed of laying hens over 70 weeks of age.

[0022] The present invention has the following beneficial effects:

[0023] 1. The additive and preparation method for improving the egg shell quality of laying hens using 25-OH-VD3, using independent domestic leading technology, creates a synthetic 25-hydroxyvitamin D3 product as the main effect product, and from the overall perspective of health and metabolism, selects and matches other synergistic active substances, and solves the production problem of deterioration of egg shell quality in modern laying hen farming from the overall concept of nutrition, immunity, health and production;

[0024] The innovation of 25-hydroxyvitamin D3 as the main nutritional immune active substance has been used in the industry to strengthen the improvement of the eggshell quality of laying hens. It has expanded the traditional use of vitamin D as the "golden partner" of calcium and phosphorus metabolism, and strengthened the practical concepts of "treating the root cause" and "treating the symptoms". Through 25-hydroxyvitamin D3, the metabolism of laying hens is adjusted, immunity is strengthened, anti-oxidation is promoted, and egg production is enhanced, thereby improving the health of laying hens and effectively improving the eggshell quality.

[0025] 2. The additive for improving the egg shell quality of laying hens using 25-OH-VD3 and its preparation method, through a unique combination of active substances, not only enhances the turnover of bone calcium in laying hens, but also improves the ability of the oviduct to form high-quality egg shells.

[0026] 3. The additive and preparation method for improving the eggshell quality of farmed eggs using 25-OH-VD3 can also meet the material needs for high-quality eggshell formation or regulation of key metabolism, thereby strengthening the important health guarantee for the formation of high-quality eggshells. The proportion of additive ingredients is continuously optimized through experiments, field observations and comparisons to form a feed additive with optimized composition and unique application effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a process flow chart of the present invention. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1: This example aims to promote the solution of the problem that the existing technology adopts normal eggshell quality improvement technology, which is difficult to cope with new problems under the new breeding model, pays little attention to the nutritional immunity measures of laying hens, making it difficult to ensure the health of laying hens, thereby making it difficult to effectively improve the eggshell quality. Please refer to Figure 1 , an additive for improving the quality of chicken egg shells using 25-OH-VD3 and a preparation method thereof, the additive comprising the following components by weight: 0.03 parts of vitamin A; 0.02 parts of α-tocopheryl acetate; 4 parts of ascorbic acid; 0.05 parts of sodium bisulfite menadione; 0.03 parts of thiamine; 0.35 parts of niacin; 0.04 parts of biotin; 0.05 parts of cobalamin; 0.01 parts of folic acid; 0.08 parts of calcium pantothenate; 0.04 parts of pyridoxine; 0.15 parts of riboflavin; 0.02 parts of inositol; 25-OH-VD3 0.15 parts; choline chloride 2.5 parts; synbiotic prebiotic compound factor 18 parts; methionine copper 0.65 parts; glycine iron 3.24 parts; methionine zinc 3.25 parts; methionine manganese 5.25 parts; calcium hydrogen phosphate 35 parts; zeolite powder 12 parts; rice husk powder 15.09 parts, the above components are mixed to prepare an additive, 2kg of the mixed additive per ton of compound feed.

[0030] The 18 parts of synbiotic prebiotic compound factors include 2.5 parts of Bacillus licheniformis, 3 parts of Bacillus subtilis, 2.5 parts of oligofructose, 2.5 parts of chitosan oligosaccharide, 5 parts of lactic acid bacteria metabolites, and 2.5 parts of synergistic factor yucca extract.

[0031] In this embodiment: using independent domestic leading technology, creating a synthetic 25-hydroxyvitamin D3 product as the main effect product, starting from the overall ideas of health and metabolism, optimizing and matching other synergistic active substances, solving the production problem of eggshell quality deterioration in modern laying hen farming from the overall concept of nutrition, immunity, health and production;

[0032] The innovation of 25-hydroxyvitamin D3 as the main nutritional immune active substance has been used in the industry to strengthen the improvement of the eggshell quality of laying hens. It has expanded the traditional use of vitamin D as the "golden partner" of calcium and phosphorus metabolism, and strengthened the practical concepts of "treating the root cause" and "treating the symptoms". Through 25-hydroxyvitamin D3, the metabolism of laying hens is adjusted, immunity is strengthened, anti-oxidation is enhanced, and egg production is enhanced, thereby improving the health of laying hens, thereby effectively improving the eggshell quality. It tries to solve the problem that the existing technology uses normal eggshell quality improvement technology, which is difficult to cope with new problems under the new breeding model, and the low attention to nutritional immunity measures for laying hens makes it difficult to ensure the health of laying hens, which leads to the problem of difficulty in effectively improving the eggshell quality.

[0033] Embodiment 2: This embodiment is another optional solution of Embodiment 1. For details, please refer to Figure 1The additives include the following components by weight: 0.02 parts of vitamin A; 0.15 parts of Dl-α-tocopheryl acetate; 3 parts of L-ascorbic acid; 0.03 parts of sodium bisulfite menadione; 0.02 parts of thiamine; 0.20 parts of niacin; 0.02 parts of biotin; 0.03 parts of cobalamin; 0.01 parts of folic acid; 0.05 parts of calcium pantothenate; 0.02 parts of pyridoxine; 0.20 parts of riboflavin; 0.01 parts of inositol; 25-OH-VD3 0.20 parts; 3 parts of choline chloride; 13 parts of synbiotic prebiotic compound factors, 0.54 parts of methionine copper; 2.49 parts of glycine iron; 2.46 parts of methionine zinc; 4.03 parts of methionine manganese; 25 parts of calcium hydrogen phosphate; 10 parts of zeolite powder; 35.52 parts of rice husk powder, mix the above components to prepare an additive, 2kg of the mixed additive per ton of compound feed.

[0034] The 13 parts of synbiotic prebiotic complex factors include 2 parts of Bacillus licheniformis, 2.5 parts of Bacillus subtilis, 1.5 parts of oligofructose, 1.5 parts of chitosan oligosaccharide, 4 parts of lactic acid bacteria metabolites, and 1.5 parts of synergistic factor yucca extract.

[0035] Embodiment 3: This embodiment is another optional solution of Embodiment 1. For details, please refer to Figure 1 The additive includes the following components by weight: 0.04 parts of vitamin A; 0.04 parts of Dl-α-tocopheryl acetate; 5 parts of L-ascorbic acid; 0.07 parts of sodium bisulfite menadione; 0.04 parts of thiamine; 0.45 parts of niacin; 0.05 parts of biotin; 0.04 parts of cobalamin; 0.02 parts of folic acid; 0.09 parts of calcium pantothenate; 0.05 parts of pyridoxine; 0.25 parts of riboflavin; 0.03 parts of inositol; 0.20 parts of 25-OH-VD3; 4 parts of choline chloride; 17 parts of synbiotic prebiotic complex factor, 0.80 parts of methionine copper; 3.51 parts of glycine iron; 3.27 parts of methionine zinc; 6.10 parts of methionine manganese; 30 parts of calcium hydrogen phosphate; 11 parts of zeolite powder; 17.95 parts of rice husk powder. The above components are mixed to prepare an additive, and the additive is mixed with 2 kg per ton of compound feed.

[0036] The 17 parts of synbiotic prebiotic compound factors include 2.6 parts of Bacillus licheniformis, 3.5 parts of Bacillus subtilis, 2 parts of oligofructose, 2 parts of chitosan oligosaccharide, 4 parts of lactic acid bacteria metabolites, and 2.9 parts of synergistic factor yucca extract.

[0037] Experiment on feeding effect of laying hens

[0038] Feeding experimental conditions and design

[0039] 3200 healthy, 56-week-old Hy-Line grey laying hens with basically the same egg-laying performance were selected. A single-factor completely randomized design was used and the experimental chickens were randomly divided into 4 treatments, including 1 control group and 3 experimental groups. Each treatment had 4 replicates, with 200 chickens in each replicate. The experimental period was 16 weeks, including a 1-week pre-test period (57-week-old laying hens) and a 15-week test period (58-72-week-old laying hens).

[0040] The experimental diet was designed with reference to the feeding standards of laying hens in my country and the nutritional level of the diet recommended by Hy-Line Company to design a corn-soybean meal type basic diet. The composition and nutritional level of the basic diet are shown in Table 1. At the beginning of the pre-trial period, the control group was fed with the basic diet in Table 1, and the three experimental groups were fed with the basic diet + the feed mixed with additives in the recommended amount of Examples 1-3.

[0041] Table 1 Composition and nutritional level of the experimental basal diet

[0042]

[0043] The experimental chicken house conditions were closed stacked small group cage technology. There were 8 chickens in each cage, with conventional feeding and management, free access to food and water, mechanical manure removal, 16 hours of light per day, and feeding 3 times a day (8:00, 11:00, 16:00).

[0044] (2) Contents of feeding experiment

[0045] During the experimental period, the number of eggs laid, egg weight, feed amount, number of chickens in stock, and number of broken eggs were recorded in replicates. The remaining feed was weighed once a week, and the egg production rate, average egg weight, average daily feed intake, feed-to-egg ratio, and egg breaking rate in the early (58-65 weeks of age) and late (66-72 weeks of age) stages were calculated.

[0046] During the test period, three normal eggs were randomly selected every weekend for two consecutive days each time, and the eggshell quality related indicators such as eggshell strength and eggshell thickness were measured, and the eggshell specific gravity and eggshell density were calculated.

[0047] Determination of serum biochemical indices: At the 7th week (65 weeks of age) and 15th week (72 weeks of age) of the experiment, the chickens were fasted, and 5 ml of blood was randomly drawn from the hearts of 3 chickens in each repetition. The syringe was placed at an angle, and the serum was separated after it precipitated naturally for detection.

[0048] Serum antioxidant enzyme system determination - Serum glutathione peroxidase (GSH-px), total superoxide dismutase (SOD), and total antioxidant enzyme (T-AOC) activities were determined using the peroxide colorimetric method with a kit (Nanjing Jiancheng Bioengineering Institute) and a 721 grating spectrophotometer (Shanghai Pudong Physical Optical Instrument Factory).

[0049] Determination of serum immunoglobulins - IgG and IgM levels were determined by enzyme-linked immunosorbent assay (ELISA) using a kit purchased from Shanghai Guduo Biotechnology Co., Ltd.

[0050] Determination of avian influenza and Newcastle disease antibody titers: At the end of the pre-test period, Newcastle disease Lasota strain vaccine (Qingdao Yibang Biotechnology Co., Ltd.) was administered through drinking water, and on the second day, a recombinant avian influenza virus H5 subtype bivalent inactivated vaccine (Nanjing Merial Animal Health Co., Ltd.) was injected intramuscularly. After vaccination, the titer of antibodies was determined by referring to the existing conventional hemagglutination inhibition test (HI), and the results are expressed as log2.

[0051] (3) Comparison of feeding experiment results

[0052] Table 2 Effects of feeding laying hens with patented additives on their production performance

[0053]

[0054] As can be seen from Table 2, compared with the conventional control group, adding the patented feed additive to the diet significantly improved the egg production rate of laying hens in the late laying period (P<0.05), reduced the egg breakage rate (P<0.01), reduced the mortality rate of laying hens (P>0.05), and had a trend of reducing the feed-egg ratio (P>0.05).

[0055] As can be seen from Table 3, compared with the conventional control group, the addition of the patented feed additive to the diet significantly improved the eggshell quality of laying hens in the late laying period. The eggshell thickness, eggshell strength and eggshell density were significantly increased (P<0.01); the eggshell specific gravity was significantly increased (P<0.01).

[0056] Table 3 Effects of feeding laying hens with patented additives on egg shell quality

[0057]

[0058] As shown in Table 4, compared with the conventional control group, the addition of the patented feed additive to the diet significantly increased the serum GSH-px, SOD and T-AOC levels of laying hens in the late laying period (P<0.05).

[0059] Table 4 Effect of patented additives on the antioxidant function of laying hens Unit (μmol / L)

[0060]

[0061]

[0062] Table 5 Effects of patented additives on Newcastle disease ND and H9 avian influenza antibody titers in laying hens

[0063]

[0064] As shown in Table 5, compared with the conventional control group, adding the patented feed additive to the diet significantly increased the Newcastle disease and H9 avian influenza antibody titers of laying hens in the late laying period, delayed the decline of avian influenza and Newcastle disease antibodies, and improved the immune function of laying hens.

[0065] (4) Conclusions of the present invention from experiments and production applications

[0066] The present invention takes the unique 25-OH-VD3 independently developed as an important feature, and develops an efficient eggshell quality improver specially used for large-scale high-density laying hen breeding. The feeding experiment is carried out according to the above-mentioned embodiments 1-3, or the product developed on this basis is promoted. 2kg is added to each ton of conventional feed to feed laying hens in the most common stacked high-density breeding mode, especially laying hens in the late egg-laying period. The experiment and industrial promotion production practice show that the unique balanced nutrition and efficient immune combination of the invention can achieve the improvement effect of the three major traits and performances of laying hens: on the one hand, the eggshell thickness, eggshell strength and eggshell density can be significantly improved; on the other hand, the egg-laying performance can be improved and the mortality rate of laying hens can be reduced; the antioxidant function of laying hens can also be strengthened, the decline of avian influenza and Newcastle disease antibodies can be delayed, and the immune function of laying hens can be improved. These technical effects fully reflect the innovation and scientificity of the present invention.

[0067] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0068] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing an additive for improving the quality of chicken egg shells using 25-OH-VD3, characterized in that: The specific steps include: S1. Prepare the following raw material components by weight: 0.10-0.40 parts of vitamin A; 0.10-0.40 parts of α-tocopheryl acetate; 2-6 parts of ascorbic acid; 0.03-0.09 parts of sodium bisulfite menadione; 0.01-0.04 parts of thiamine; 0.20-0.50 parts of niacin; 0.01-0.06 parts of biotin; 0.02-0.08 parts of cobalamin; 0.01-0.02 parts of folic acid; 0.05-0.13 parts of calcium pantothenate; 0.02-0.07 parts of pyridoxine; 0.10-0.20 parts of riboflavin; 0.01-0.03 parts of inositol; 0.10-0.20 parts of 25-OH-VD3; 1-4 parts of choline chloride; 9-19 parts of synbiotic prebiotic factors, 0.54-0.86 parts of copper methionine; 2.49-3.81 parts of iron glycine; 2.46-3.77 parts of zinc methionine; 4.03-6.14 parts of manganese methionine; 20-45 parts of calcium hydrogen phosphate; 8-12 parts of zeolite powder; 7.12-43.20 parts of rice husk powder; S2. The above raw material components are stirred and mixed to prepare an additive.

2. The method for preparing an additive for improving the quality of chicken egg shells using 25-OH-VD3 according to claim 1, characterized in that: The preparation method of the synbiotic prebiotic factor of 9-19 parts is as follows: 1.5-3 parts of Bacillus licheniformis; 2-4 parts of Bacillus subtilis; 1-4 parts of oligofructose; 1-4 parts of chitosan oligosaccharide; 4-6 parts of lactic acid bacteria metabolites; and 1-3 parts of synergistic factor yucca extract, and the mixture is stirred and evenly mixed.

3. The method for preparing an additive for improving the quality of chicken egg shells using 25-OH-VD3 according to claim 1, characterized in that: The specific steps include: S1. Prepare the following raw material components by weight: 0.03 parts of vitamin A; 0.02 parts of Dl-α-tocopheryl acetate; 4 parts of L-ascorbic acid; 0.05 parts of sodium bisulfite menadione; 0.03 parts of thiamine; 0.35 parts of niacin; 0.04 parts of biotin; 0.05 parts of cobalamin; 0.01 parts of folic acid; 0.08 parts of calcium pantothenate; 0.04 parts of pyridoxine; 0.15 parts of riboflavin; 0.02 parts of inositol; 0.15 parts of 25-OH-VD3; 2.5 parts of choline chloride; 18 parts of synbiotic prebiotic factors; 0.65 parts of copper methionine; 3.24 parts of glycine iron; 3.25 parts of methionine zinc; 5.25 parts of methionine manganese; 35 parts of calcium hydrogen phosphate; 12 parts of zeolite powder; 15.09 parts of rice husk powder; S2. The above raw material components are stirred and mixed to prepare an additive.

4. The method for preparing an additive for improving the quality of chicken egg shells using 25-OH-VD3 according to claim 3, characterized in that: The preparation method of 18 parts of the synbiotic prebiotic factor is as follows: 2.5 parts of Bacillus licheniformis; 3 parts of Bacillus subtilis; 2.5 parts of oligofructose, 2.5 parts of chitosan oligosaccharide, 5 parts of lactic acid bacteria metabolites, and 2.5 parts of synergistic factor yucca extract are stirred and mixed.

5. The method for preparing an additive for improving the quality of chicken egg shells using 25-OH-VD3 according to claim 1, characterized in that: The purity of the 25-OH-VD3 is ≥94%, and it is an activated vitamin D product.

6. An additive for improving the quality of chicken egg shells using 25-OH-VD3, a method for preparing an additive for improving the quality of chicken egg shells using 25-OH-VD3 according to claims 1-5, characterized in that: 2 kg of the additive is added per ton of compound feed, and the additive can be increased by 10%-30% in the feed of laying hens over 70 weeks of age.

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