Co-production method of chicken bone oil, chondroitin sulfate and bone collagen

Abstract

The invention relates to the technical field of animal cartilage processing and extraction, and particularly discloses a co-production method of chicken bone oil, chondroitin sulfate and bone collagen. A co-production method of chicken bone oil, chondroitin sulfate and bone collagen comprises the following specific steps: (1) preparing raw materials; (2) performing primary high-pressure steam direct steaming: performing primary high-pressure steam direct steaming on pretreated chicken bone residues, and quickly deoiling to obtain chicken bone oil and deoiled chicken bone residues; (3) secondary interlayer cooking: carrying out secondary interlayer cooking on the deoiled chicken bone residues, firstly boiling with big fire, and then slowly cooking with slow fire at 60-80 DEG C to obtain a mixed solution of chondroitin sulfate and chicken bone collagen; and (4) separating and purifying to obtain bone collagen and chondroitin sulfate. According to the method, the chicken bone oil, the chondroitin sulfate and the bone collagen are effectively separated and extracted through the combination of all the processes, and meanwhile the chondroitin sulfate and the bone collagen have good purity.

Description

Technical Field

[0001] The present application relates to the technical field of animal cartilage processing and extraction, and in particular to a method for co-producing chicken bone oil, chondroitin sulfate and bone collagen. Background Art

[0002] Chicken bone residue is a byproduct obtained after the separation of chicken skeleton from bone and meat. It is an important byproduct produced during the slaughter and cutting process of broiler chickens. The collagen content in chicken bones is ≥20%, and the bone oil content is ≥15%. It also contains many functional ingredients, such as chondroitin sulfate and other mucopolysaccharides, which are nutritional and health care ingredients required by the human body and are an important source of animal protein. At present, there are two main ways to process chicken bone residue. One is to simply dry and crush it into bone meal, which is used for livestock and poultry feed, aquaculture, etc.; the other is to extract it into chicken bone essence, which is used in seasonings, bone soup, salty food flavors and other channels.

[0003] Although chicken bone residue is currently being utilized, its value has not been maximized. Due to the current processing method, the bone oil cannot be completely separated, resulting in the inability to produce collagen powder with a protein content of more than 95% and chondroitin sulfate products with a protein content of ≥ 90%. At the same time, the chicken bone oil cannot be completely separated, which also leads to a short shelf life of the product. Summary of the invention

[0004] In order to improve the problem of incomplete bone oil separation in the existing chicken bone residue processing and increase the content of bone collagen and chondroitin sulfate, the present application provides a method for co-producing chicken bone oil, chondroitin sulfate and bone collagen.

[0005] The present application provides a method for co-producing chicken bone oil, chondroitin sulfate and collagen, which adopts the following technical scheme: A method for co-producing chicken bone oil, chondroitin sulfate and collagen comprises the following specific steps: (1) Raw material preparation: washing, crushing and drying the chicken bone residue; (2) direct steaming with high pressure steam: direct steaming the pretreated chicken bone residue with high pressure steam to quickly remove oil, thereby obtaining chicken bone oil and deoiled chicken bone residue; (3) Secondary sandwich cooking: The de-oiled chicken bone residue is subjected to secondary sandwich cooking, firstly boiled over high heat, and then slowly cooked over low heat at 60-80° C. to obtain a mixture of chondroitin sulfate and chicken bone collagen; (4) Separation and purification: The mixed solution of chondroitin sulfate and chicken collagen is subjected to enzymatic hydrolysis and filtration to obtain a concentrated solution, the concentrated solution is adsorbed on a resin column to obtain an extract, the extract is subjected to ultrafiltration and concentration, spray drying to obtain collagen, the resin column is eluted to obtain an eluate, the eluate is subjected to ultrafiltration and concentration, alcohol precipitation, dehydration, and drying to obtain chondroitin sulfate.

[0006] By adopting the above technical scheme, through the combination of primary high-pressure steam direct steaming and secondary interlayer cooking, separation and purification process steps, chicken bone oil, chondroitin sulfate and collagen can be efficiently separated and extracted from chicken bone residue, so that the three extract products have a longer shelf life.

[0007] High-pressure steam can quickly penetrate the chicken bone residue, heat the internal fat, and promote the rapid separation of fat due to temperature difference, which helps to efficiently extract fat from chicken bones and improve the extraction rate of chicken bone oil. At the same time, high-pressure steam can also soften the bone, which is conducive to the subsequent separation and extraction of chondroitin sulfate and collagen.

[0008] In the secondary layer cooking, first boil over high heat to quickly dissolve the outer layer, then simmer over low heat to maintain the structural integrity of chondroitin sulfate at a lower temperature, reduce thermal degradation, and facilitate the retention and extraction of the active ingredients of chondroitin sulfate. At the same time, simmering over low heat can help hydrolyze collagen and convert it into a more water-soluble state, thereby increasing the solubility and extraction of collagen.

[0009] Preferably, the enzyme used in the enzymolysis process is one of papain and neutral protease, and the enzyme dosage is 2-10 g / kg.

[0010] By adopting the above technical solution, papain and neutral protease are proteases with specific cleavage sites, which can specifically break the peptide chain of the protein without destroying chondroitin sulfate, which is conducive to separating and purifying collagen from the mixed solution and improving the extraction efficiency of chondroitin sulfate and collagen. At the same time, during the enzymatic hydrolysis process, papain and neutral protease can reduce protein impurities, retain more biological activity of the product, and improve the purity of chondroitin sulfate and collagen.

[0011] Preferably, the enzymolysis temperature is 35-50°C.

[0012] Preferably, the enzymatic filtration uses an ultrafiltration membrane with a molecular weight cutoff of 9-10 kDa, and the ultrafiltration process uses an ultrafiltration membrane with a molecular weight cutoff of 1-3 kDa.

[0013] By adopting the above technical solution, the ultrafiltration membrane with a molecular weight cutoff of 9-10kDa is used to ultrafilter and concentrate the extract, which can remove small molecule impurities such as small molecule peptides and inorganic salts, and improve the purity of collagen. The ultrafiltration membrane with a molecular weight cutoff of 1-3kDa is used to ultrafilter and concentrate the extract and eluate, which can remove small molecule impurities while concentrating the target macromolecular substances, maintain the activity of the target biological molecules, and improve the purity of chondroitin sulfate and collagen.

[0014] Preferably, the resin column is eluted with a sodium chloride solution, and the concentration of the sodium chloride is 1-5 mol / L.

[0015] By adopting the above technical solution, using sodium chloride solution for elution can promote the desorption of biological macromolecules such as proteins on the resin column, making the proteins easier to elute, while also stabilizing the protein structure and improving the purity and extraction efficiency of chondroitin sulfate.

[0016] Preferably, the resin used for the resin column adsorption is an anion exchange resin.

[0017] By adopting the above technical solution, compared with other resins, the use of anion exchange resin can have a higher affinity for molecules with negative charges such as chondroitin sulfate, which makes chondroitin sulfate easy to be adsorbed by the anion exchange resin, effectively separates chondroitin sulfate from complex mixtures, and improves the extraction efficiency of chondroitin sulfate. At the same time, the anion exchange resin can adsorb and retain chondroitin sulfate during the adsorption process, while the charge characteristics of collagen are lower than chondroitin sulfate, and the degree of adsorption by the resin is lower, thereby separating chondroitin sulfate from collagen, and improving the purity of chondroitin sulfate and collagen extraction products respectively.

[0018] Preferably, the anion exchange resin comprises the following raw materials in parts by weight: 40-60 parts of p-ethylstyrene, 20-30 parts of divinylbenzene, 0.5-1 parts of initiator, 80-100 parts of porogen, and 1-3 parts of dispersant.

[0019] By adopting the above technical scheme, divinylbenzene and p-ethylstyrene are cross-linked, so that the prepared anion exchange resin has higher strength and structural stability, and can maintain good structural integrity of the anion exchange resin during repeated adsorption and elution processes, reduce resin breakage and decreased adsorption performance, and improve the adsorption effect and adsorption stability of the anion exchange resin.

[0020] Preferably, the dispersant is a mixture of polyvinyl alcohol and sodium polyphosphate, and the porogen is a mixture of butanol and dodecanol.

[0021] By adopting the above technical scheme, polyvinyl alcohol and sodium polyphosphate are compounded as dispersants, which can reduce the surface tension of deionized water, make divinylbenzene and p-ethylstyrene more easily form small liquid droplets in water, and adsorb on the surface of the small liquid droplets to form a protective layer, reduce the merging or adhesion between the small liquid droplets, and improve the dispersibility of the anion exchange resin system. Butanol and dodecanol are compounded as porogens. Dodecanol can maintain the mesopore ratio of divinylbenzene-p-ethylstyrene copolymers and increase the adsorption rate of anion exchange resins. Butanol can increase the small pore ratio of divinylbenzene-p-ethylstyrene copolymers and increase the specific surface area and adsorption capacity of anion exchange resins. Butanol and dodecanol compound can promote the anion exchange resin to have a better adsorption effect.

[0022] Preferably, the preparation method of the anion exchange resin comprises the following specific steps: mixing p-ethylstyrene, divinylbenzene, an initiator and water, heating to 40-60°C, adding a dispersant, stirring evenly to form suspended droplets, solidifying into balls, then heating to 70-90°C for constant temperature reaction for 3-6 hours, cooling and washing with water, filtering and drying to obtain poly (p-ethylstyrene-divinylbenzene) copolymer crosslinked microspheres, and then sequentially chloromethylating and aminated with trimethylamine to obtain the anion exchange resin.

[0023] By adopting the above technical solution, the chloromethyl group is connected to the resin polymer chain, which acts as a bridge for subsequent amination, prompting the chloromethyl group on the resin to be converted into a quaternary ammonium salt, which can then exchange and adsorb negatively charged ions, thereby improving the adsorption effect of the effective components of chondroitin sulfate, enhancing the adsorption effect and adsorption capacity of the anion exchange resin, and improving the yield and purity of chondroitin sulfate.

[0024] Preferably, the chicken bone oil has an acid value of ≤2.5 mg / kg and a peroxide value of ≤0.2 g / 100 g; the protein content in the collagen is ≥75%, the fat content in the collagen is ≤1%, and the water content in the collagen is ≤5%; and the chondroitin sulfate content in the chondroitin sulfate product is ≥90%.

[0025] In summary, this application has the following beneficial effects: 1. Since the high-pressure steam of the present application quickly separates the fat from the chicken bones, the influence of the fat on the subsequent process is reduced, and the extract product has a longer shelf life. The use of sandwich cooking, first boiling over high heat and then simmering over low heat, is conducive to the separation of collagen, while maintaining the structural integrity of chondroitin sulfate, thereby improving the extraction of chondroitin sulfate and collagen.

[0026] 2. In this application, anion exchange resin chondroitin sulfate is used to effectively adsorb and separate from a complex mixture, and the structural strength and integrity of the anion exchange resin are enhanced by cross-linking divinylbenzene with p-ethylstyrene. Polyvinyl alcohol and sodium polyphosphate are used as dispersants to improve the dispersibility of the anion exchange resin system, and butanol and dodecanol are used to maintain the mesopore ratio of the anion exchange resin, thereby increasing the specific surface area and adsorption capacity of the anion exchange resin. DETAILED DESCRIPTION

[0027] The present application is further described in detail below in conjunction with embodiments.

[0028] All raw materials in the examples are commercially available.

[0029] The molecular weight of polyvinyl alcohol is 120,000.

[0030] Sodium polyphosphate is sodium tripolyphosphate.

[0031] Preparation Example of Anion Exchange Resin Preparation Example 1 The anion exchange resin comprises the following raw materials in parts by weight: 50 kg of p-ethylstyrene, 25 kg of divinylbenzene, 0.8 kg of initiator, 90 kg of porogen, and 2 kg of dispersant, wherein the initiator is benzoyl peroxide, the porogen is butanol, and the dispersant is polyvinyl alcohol.

[0032] The preparation method of anion exchange resin comprises the following specific steps: Para-ethylstyrene, divinylbenzene and an initiator are formed into a mixture, which is then added into water in a mass ratio of 3:1. After heating to 50°C, a dispersant is added, stirred evenly to form suspended droplets, and then the temperature is raised to 70°C for constant temperature reaction for 3 hours, and then the temperature is further raised to 90°C for insulation reaction for 3 hours, and then the temperature is lowered to 40°C, washed with water, filtered, and dried to obtain poly (para-ethylstyrene-divinylbenzene) copolymer crosslinked microspheres, and then the poly (para-ethylstyrene-divinylbenzene) copolymer crosslinked microspheres are chloromethylated to obtain chloromethylated copolymer crosslinked microspheres, and then aminated with trimethylamine to obtain anion exchange resin.

[0033] Preparation Example 2 The difference between Preparation Example 2 and Preparation Example 1 is that the amount of p-ethylstyrene used in the anion exchange resin raw material is 40 kg, the amount of divinylbenzene used is 20 kg, the amount of initiator used is 0.5 kg, the amount of porogen used is 80 kg, and the amount of dispersant used is 1 kg.

[0034] Preparation Example 3 The difference between Preparation Example 3 and Preparation Example 1 is that the amount of p-ethylstyrene used in the anion exchange resin raw material is 60 kg, the amount of divinylbenzene used is 30 kg, the amount of initiator used is 1 kg, the amount of porogen used is 100 kg, and the amount of dispersant used is 3 kg.

[0035] Preparation Example 4 The difference between Preparation Example 4 and Preparation Example 1 is that the dispersant in the anion exchange resin raw material is sodium polyphosphate.

[0036] Preparation Example 5 The difference between Preparation Example 5 and Preparation Example 1 is that the dispersant in the anion exchange resin raw material is a mixture of sodium polyphosphate and polyvinyl alcohol, and the mass ratio of sodium polyphosphate to polyvinyl alcohol is 1:1.

[0037] Preparation Example 6 The difference between Preparation Example 6 and Preparation Example 1 is that the porogen in the anion exchange resin raw material is a mixture of butanol and dodecanol, and the mass ratio of butanol to dodecanol is 1:1. Example

[0038] Example 1 This embodiment provides a method for co-producing chicken bone oil, chondroitin sulfate and collagen, comprising the following specific steps: (1) Wash the chicken bone residue with water, crush it into chicken bone residue powder with an average particle size of 0.1-1 mm, dry it and set it aside.

[0039] (2) The pretreated chicken bone residue is subjected to high-pressure steam direct steaming at a pressure of 12 MPa and a temperature of 110° C. for 3 h to quickly remove oil, thereby obtaining chicken bone oil and deoiled chicken bone residue.

[0040] (3) The de-oiled chicken bone residue is subjected to secondary interlayer cooking, firstly boiled over high heat for 1 h, and then slowly cooked at 70° C. for 2 h to obtain a mixture of chondroitin sulfate and chicken bone collagen.

[0041] (4) Add papain to the mixed solution of chondroitin sulfate and chicken collagen for enzymolysis, wherein the papain is selected from Guangxi Nanning Pangbo Bioengineering Co., Ltd., and the amount of the enzyme used is 6 g / kg of the mixed solution. The enzymolysis is carried out at 45° C. for 3 h to obtain an enzymolysis solution, and the enzymolysis solution is filtered through an ultrafiltration membrane with a molecular weight cutoff of 9 kDa to obtain a concentrated solution, and the concentrated solution is adsorbed on a resin column to obtain an extract, wherein the resin used in the resin column adsorption process is D001 styrene-based macroporous cation exchange resin, and the extract is ultrafiltered and concentrated through an ultrafiltration membrane with a molecular weight cutoff of 3 kDa, and then spray-dried to obtain collagen.

[0042] (5) The resin column is eluted twice with a sodium chloride aqueous solution having a concentration of 3 mol / L to obtain an eluate, the eluate is concentrated by ultrafiltration through an ultrafiltration membrane having a molecular weight cutoff of 1 kDa, and the filtered eluate is precipitated with ethanol, the precipitate is collected, dehydrated and dried to obtain a chondroitin sulfate product.

[0043] Example 2 The difference between Example 2 and Example 1 is that the enzyme used in the enzymatic hydrolysis in the co-production method of chicken bone oil, chondroitin sulfate and collagen is a neutral protease, and the neutral protease is selected from Guangxi Nanning Pangbo Bioengineering Co., Ltd.

[0044] Example 3 The difference between Example 3 and Example 1 is that in step (4) of the method for co-production of chicken bone oil, chondroitin sulfate and collagen, the extract is concentrated by ultrafiltration through an ultrafiltration membrane with a molecular weight cutoff of 9 kDa.

[0045] Example 4 The difference between Example 4 and Example 1 is that the resin used for resin column adsorption in the method for co-producing chicken bone oil, chondroitin sulfate and collagen comes from Preparation Example 1.

[0046] Example 5 The difference between Example 5 and Example 1 is that the resin used for resin column adsorption in the co-production method of chicken bone oil, chondroitin sulfate and collagen comes from Preparation Example 2.

[0047] Example 6 The difference between Example 6 and Example 1 is that the resin used for resin column adsorption in the co-production method of chicken bone oil, chondroitin sulfate and collagen comes from Preparation Example 3.

[0048] Example 7 The difference between Example 7 and Example 1 is that the resin used for resin column adsorption in the co-production method of chicken bone oil, chondroitin sulfate and collagen comes from Preparation Example 4.

[0049] Example 8 The difference between Example 8 and Example 1 is that the resin used for resin column adsorption in the co-production method of chicken bone oil, chondroitin sulfate and collagen comes from Preparation Example 5.

[0050] Example 9 The difference between Example 9 and Example 1 is that the resin used for resin column adsorption in the co-production method of chicken bone oil, chondroitin sulfate and collagen comes from Preparation Example 6.

[0051] Comparative Example Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that in step (2) of the method for co-producing chicken bone oil, chondroitin sulfate and collagen, high heat is directly used for boiling for 3 hours.

[0052] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that in step (2) of the method for co-producing chicken bone oil, chondroitin sulfate and bone collagen, the mixed solution of chondroitin sulfate and chicken bone collagen is not filtered after enzymatic hydrolysis.

[0053] Performance testing According to the co-production method of chicken bone oil, chondroitin sulfate and collagen provided in Examples 1-9 and Comparative Examples 1-2 of the present application, the prepared chicken bone oil, collagen and chondroitin sulfate were subjected to the following performance tests, and the specific test results are shown in Table 1.

[0054] Detection Methods 1. Collagen product testing The moisture and fat content of the collagen prepared in this application was detected by referring to the inspection method of the physical and chemical indicators of GB31645-2018 "National Food Safety Standard Collagen Peptide". The protein content in the collagen prepared in this application was detected by the Lowry method.

[0055] 2. Quantitative Analysis of Chondroitin Sulfate Products The commercially available chondroitin sulfate standard was used as a control, and the peak time was basically consistent with that of the standard. The content of chondroitin sulfate in the chondroitin sulfate product (%) = the mass of chondroitin sulfate in the product / the mass of the product (dry product) × 100, to detect the purity of the chondroitin sulfate product prepared in this application.

[0056] 3. Chicken bone oil product testing The acid value of chicken bone oil samples was determined by the cold solvent indicator titration method in accordance with GB5009.229-2016 "National Food Safety Standard for Determination of Acid Value in Foods". The peroxide value of chicken bone oil samples was determined by the titration method in accordance with GB 5009.227-2016 "National Food Safety Standard for Determination of Peroxide Value in Foods".

[0057] Table 1: Performance test results data table It can be seen from the performance test results that the present application can effectively separate and extract chicken bone oil, chondroitin sulfate and collagen from chicken bone residue through the combination of various processes. At the same time, the extracted chicken bone oil has a lower acid value and peroxide value, which enables the extract product to have a longer shelf life.

[0058] By comparing Example 3 with Example 1, it can be seen that in the co-production method of chicken bone oil, chondroitin sulfate and collagen, the extract is ultrafiltered and concentrated using ultrafiltration membranes of different sizes. From the performance test results, it can be seen that the ultrafiltration membrane used in Example 1 is more suitable. The ultrafiltration membrane with a smaller molecular weight cutoff used in Example 1 can more effectively remove small molecule impurities, while increasing the concentration of the target molecular substance and improving the product purity.

[0059] By comparing Example 1 with Examples 4-6, it can be seen that Examples 4-6 use anion exchange resin, and from the performance test results, it can be seen that the purity of chondroitin sulfate and collagen in Example 4 is significantly improved, and the impurity content in the collagen product is reduced. It further illustrates that the anion exchange resin can effectively adsorb and retain chondroitin sulfate, while reducing the adsorption degree of collagen, effectively separating chondroitin sulfate and collagen, and improving the purity of chondroitin sulfate and collagen products.

[0060] By comparing Examples 7-8 with Example 4, it can be seen that different types of dispersants in anion exchange resins will also affect the purity of chondroitin sulfate and collagen products, among which the purity of chondroitin sulfate and collagen products in Example 8 is higher. It further illustrates that the compounding of sodium polyphosphate and polyvinyl alcohol can successfully disperse the resin microspheres, reduce the adhesion of the resin balls, and thus improve the adsorption capacity and adsorption effect of the anion exchange resin.

[0061] By comparing Example 9 with Example 1, it can be seen that Example 9 uses a different porogen. From the performance test results, it can be seen that the porogen in Example 9 uses a compound of butanol and dodecanol, which can maintain the ratio of pore sizes in the anion exchange resin, thereby improving the adsorption effect of the anion exchange resin, and improving the extraction effect and product purity of chondroitin sulfate and collagen.

[0062] By comparing Comparative Examples 1-2 with Example 1, it can be seen that in Comparative Example 1, long-term high-fire boiling is used instead of the process of first high-fire boiling and then slow-cooking. From the performance test results, it can be seen that the impurity content in chondroitin sulfate and collagen products is significantly increased, and the purity of chondroitin sulfate is reduced, further indicating that the structure of chondroitin sulfate is destroyed during long-term high-fire boiling, and chondroitin sulfate and collagen cannot be effectively separated. In Comparative Example 2, the enzymatic hydrolyzate is not filtered, resulting in an increase in the impurity content in the collagen, and small molecule impurities cannot be effectively removed, reducing the purity of the product.

[0063] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for co-producing chicken bone oil, chondroitin sulfate and collagen, characterized in that: The specific steps include: (1) Raw material preparation: Wash, crush and dry the chicken bone residue; (2) One-time high-pressure steam direct steaming: The pretreated chicken bone residue is subjected to one-time high-pressure steam direct steaming to quickly remove oil, thereby obtaining chicken bone oil and de-oiled chicken bone residue; (3) Secondary sandwich cooking: The de-oiled chicken bone residue is subjected to secondary sandwich cooking, firstly boiled over high heat, and then slowly cooked over low heat at 60-80°C to obtain a mixture of chondroitin sulfate and chicken bone collagen; (4) Separation and purification: The mixed solution of chondroitin sulfate and chicken collagen is subjected to enzymatic hydrolysis and filtration to obtain a concentrated solution, the concentrated solution is adsorbed on a resin column to obtain an extract, the extract is subjected to ultrafiltration and concentration, spray drying to obtain collagen, the resin column is eluted to obtain an eluate, the eluate is subjected to ultrafiltration and concentration, alcohol precipitation, dehydration, and drying to obtain chondroitin sulfate.

2. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 1, characterized in that: The enzyme used in the enzymolysis process is one of papain and neutral protease, and the enzyme dosage is 2-10g / kg.

3. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 2, characterized in that: The enzymolysis temperature is 35-50°C.

4. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 1, characterized in that: The enzymatic filtration uses an ultrafiltration membrane with a molecular weight cutoff of 9-10 kDa, and the ultrafiltration process uses an ultrafiltration membrane with a molecular weight cutoff of 1-3 kDa.

5. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 1, characterized in that: The resin column is eluted with a sodium chloride solution, and the concentration of the sodium chloride is 1-5 mol / L.

6. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 1, characterized in that: The resin used in the resin column adsorption is an anion exchange resin.

7. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 6, characterized in that: The anion exchange resin comprises the following raw materials in parts by weight: 40-60 parts of p-ethylstyrene, 20-30 parts of divinylbenzene, 0.5-1 parts of initiator, 80-100 parts of porogen and 1-3 parts of dispersant.

8. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 7, characterized in that: The dispersant is a mixture of polyvinyl alcohol and sodium polyphosphate, and the porogen is a mixture of butanol and dodecanol.

9. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 7, characterized in that: The preparation method of the anion exchange resin comprises the following specific steps: mixing p-ethylstyrene, divinylbenzene, an initiator and water, heating to 40-60°C, adding a dispersant, stirring evenly to form suspended droplets, solidifying into balls, then heating to 70-90°C for constant temperature reaction for 3-6 hours, cooling down, washing with water, filtering and drying to obtain poly(p-ethylstyrene-divinylbenzene) copolymer crosslinked microspheres, and then sequentially chloromethylating and aminating with trimethylamine to obtain the anion exchange resin.

10. The method for co-producing chicken bone oil, chondroitin sulfate and collagen according to claim 1, characterized in that: The chicken bone oil has an acid value of ≤2.5mg / kg and a peroxide value of ≤0.2g / 100g; the protein content in the collagen is ≥75%, the fat content in the collagen is ≤1%, and the water content in the collagen is ≤5%; and the chondroitin sulfate content in the chondroitin sulfate product is ≥90%.

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