Chip direct insertion type nucleic acid amplification detection portable instrument and method

Abstract

The invention discloses a chip direct insertion type portable nucleic acid amplification detection instrument and method. The main control module is electrically connected with the heating module and the rotating module, the heating module is arranged in the chip direct insertion table, the chip direct insertion table is installed on the rotating module, and the heating module comprises a heat conduction material, a heating element and a temperature detection module; the micro-fluidic chip is inserted into the chip direct insertion table, the main control module controls the rotating module to drive the micro-fluidic chip direct insertion table to be located on the horizontal plane, when nucleic acid amplification heating is conducted on the micro-fluidic chip, the main control module controls the heating module to heat a heat conduction material and transfer the heat conduction material to the micro-fluidic chip, and the temperature detection module monitors the temperature in real time; and finally, rotating 90 degrees to enable the liquid to flow into a detection chamber with a test strip under gravity. According to the invention, integration of nucleic acid amplification and nucleic acid detection processes is realized, chip placement, temperature control and rotation are integrated in one system, and the system is suitable for rapid field detection, controllable in temperature, convenient and rapid in treatment, labor-saving and material-saving, small in size and convenient to carry.

Description

Technical Field

[0001] The present invention relates to a nucleic acid amplification detection instrument in the field of biological detection technology, and in particular to a portable nucleic acid amplification detection instrument and method that can be directly inserted into a microfluidic chip and automatically rotate, which is applicable to a variety of nucleic acid amplification technologies and is combined with microcomputer control to form an automated detection system and method. Background Art

[0002] Polymerase Chain Reaction (PCR) is a molecular biology technique used to replicate and amplify DNA molecules in vitro. PCR was invented by American biologist Kary Mullis in the early 1980s and is well-known for its wide application in molecular biology, genetics, and biomedical research. The basic principle of PCR is to continuously replicate a small piece of DNA and amplify it into millions or even billions of copies, so as to obtain enough DNA for further analysis and research. The key to PCR technology lies in the change of temperature, and the temperature cycle of three states is the core of the PCR process.

[0003] The isothermal nucleic acid amplification technology that has emerged in recent years has gotten rid of the requirement for temperature cyclic changes and has broad application prospects in the field of rapid nucleic acid amplification detection. For most portable instruments, it is a more popular choice. However, at present, isothermal nucleic acid amplification technology and PCR technology only have their own advantages and disadvantages. In many practical application scenarios, it is necessary to consider which amplification technology is more in line with the actual situation. Most portable detection instruments can only support isothermal amplification reactions. Users have little choice and it is difficult to cover many application scenarios.

[0004] Microfluidics is a commonly used control method in portable nucleic acid amplification instruments. This technology combines nucleic acid amplification technology and microfluidics, and supports nucleic acid amplification by controlling liquid flow on a microscale through structures such as valves or flow channels. This technology usually needs to be implemented through microfluidic chips or microfluidic devices, which can achieve efficient, fast and automated nucleic acid amplification. At present, microcomputer control technology is very mature, and its application in various automation devices is also common. The actuator can be adjusted and controlled by sending different duty cycle signals by the microcomputer. The rotating mechanism device is a device that can receive an external PWM signal and then rotate at a corresponding angle. It can be used in scenarios where rotation needs to be controlled. However, considering the overall mechanical structure, how to conveniently install and place the chip, and the need for portable on-site operation, the relevant structural form still needs to be implemented.

[0005] For microfluidic devices, the core of controlling the flow state of liquid lies in the driving force of the liquid. There are centrifugal force drive, pump drive, gravity drive, etc. for driving liquid. Among them, gravity drive does not require additional driving equipment and is more suitable for miniaturized and portable detection environments. However, gravity-driven microfluidic chips often need to be rotated to control the flow of liquid. This process mostly requires manual operation. Since the reaction heating time of the nucleic acid amplification process is relatively long, if manpower is required to wait for the reaction process to end before rotating, it will take a lot of time. In addition, when using the chip, it is necessary to ensure that it can be fixed during rotation, and to ensure that it is easy to place and plug in. In these aspects, the current portable nucleic acid detection instruments still have shortcomings. Summary of the invention

[0006] The purpose of the present invention is to address the deficiencies in the prior art and provide a device system and method for nucleic acid sample detection based on nucleic acid amplification technology, a microfluidic chip, which is rotatable and temperature-controllable, and combines mechanical and electronic technology.

[0007] To achieve the above object, the technical solution adopted by the present invention includes:

[0008] 1. A portable chip-based nucleic acid amplification detection instrument:

[0009] The instrument comprises a main control module, a heating module, a chip plug-in platform and a rotating module; the main control module is electrically connected to the heating module and the rotating module respectively, the heating module is arranged in the chip plug-in platform, and the chip plug-in platform is installed on the rotating module; the heating module comprises a thermal conductive material, a heating element and a temperature detection module, the thermal conductive material is arranged in the chip plug-in platform, the heating element is located on the side of the thermal conductive material installation groove of the chip plug-in platform, the temperature detection module is installed in the temperature detection module installation hole of the thermal conductive material, and the heating element, the temperature detection module and the main control module are electrically connected.

[0010] The main control module includes a main control chip, a display module, a keyboard module, a power module and a relay module; the main control chip is electrically connected to the display module, the keyboard module and the relay module respectively, the display module is electrically connected to the keyboard module, the power module is electrically connected to the main control chip, the display module, the keyboard module and the relay module, and the relay module is electrically connected to the heating element.

[0011] The chip plug-in platform is provided with a chip plug-in slot and a heat-conducting material installation slot; a chip plug-in slot is provided on one side of the bottom, a microfluidic chip is inserted in the chip plug-in slot, a heat-conducting material installation slot is provided in the middle position of the bottom, the heat-conducting material installation slot is arranged inside the heat-conducting material installation slot with heat-conducting material of the heating module, and heat-insulating material is provided around the heat-conducting material installation slot, the chip plug-in slot is connected with the heat-conducting material installation slot, the front side of the bottom of the microfluidic chip extends into the heat-conducting material installation slot and is in contact with and connected to the heat-conducting material in the heat-conducting material installation slot; a heating element is provided on the side of the heat-conducting material installation slot, and a plug-in platform fixing hole for fixing the heat-conducting material and a plug-in platform installation hole for installing the temperature detection module in the heating module are provided on the front side of the heat-conducting material installation slot; the outer wall of the chip plug-in platform is provided with a rotating mechanism fixing row of holes for connecting with the rotating mechanism.

[0012] The thermal conductive material is formed into a U shape and placed in a thermal conductive material installation groove. The opening of the U shape of the thermal conductive material serves as a chip wrapping groove. The front side of the bottom of the microfluidic chip is inserted into the chip wrapping groove and is in contact with the thermal conductive material. Sensor mounting holes and thermal conductive material fixing holes are respectively provided on both sides of the U shape of the thermal conductive material. The sensor mounting hole is used to install the temperature detection module of the heating module, and the thermal conductive material fixing hole is a screw hole for installing bolts to fix the thermal conductive material in the thermal conductive material installation groove.

[0013] The rotating module comprises a rotating mechanism and a carrier frame; the rotating mechanism is mounted on the carrier frame, the rotating mechanism is electrically connected to the main control module, and the rotating part of the rotating mechanism is fixedly connected to the chip plug-in platform through screws.

[0014] A rotating mechanism mounting groove is provided on the carrier frame, and the rotating mechanism is installed in the rotating mechanism mounting groove. A strip groove is provided at the bottom of the rotating mechanism mounting groove, and a carrier frame mounting hole for mounting the rotating mechanism is provided on the groove wall of the strip groove; the rotating mechanism includes a steering gear module, and a swivel arm is fixedly connected to the output shaft of the steering gear module, and a strip protrusion is provided at the bottom of the steering gear module, and a rotating mechanism mounting hole for mounting the steering gear module on the carrier frame is provided with the strip protrusion; the steering gear module is installed in the rotating mechanism mounting groove, and the strip protrusion is inserted into the strip groove, and the steering gear module is fixedly installed on the carrier frame by passing bolts through the rotating mechanism mounting hole and the carrier frame mounting hole.

[0015] 2. A chip-based direct-insertion nucleic acid amplification detection method:

[0016] Insert the microfluidic chip loaded with reagents into the chip slot of the chip insert station and the chip wrapping slot of the thermal conductive material. The main control module controls the rotating module to drive the microfluidic chip insert station to a horizontal plane.

[0017] When the front part of the microfluidic chip needs to be heated for nucleic acid amplification, the main control module controls the heating element in the heating module to heat the heat-conducting material, and the heat-conducting material transfers the heat to the front part of the microfluidic chip. During the heating process, the main control module controls the temperature detection module in the heating module to monitor the temperature of the heat-conducting material and the environment in real time.

[0018] After a period of time, the main control module controls the rotating module to drive the microfluidic chip plug-in table to rotate 90 degrees, so that the amplified liquid in the microfluidic chip flows into the detection chamber containing the test strip inside the chip by gravity for test strip detection.

[0019] The beneficial effects of the present invention are:

[0020] The present invention realizes the integration of nucleic acid amplification and nucleic acid detection processes, integrates a chip placement device, a temperature control device and a rotation device into one system, and is suitable for rapid on-site detection based on a microfluidic chip.

[0021] The present invention supports direct insertion of chips, and the structure is stable during the rotation process, which is convenient for chip placement and repeated experiments.

[0022] The present invention supports two nucleic acid amplification temperature control strategies, namely, constant temperature amplification and variable temperature amplification, so that users can freely adjust and select appropriate nucleic acid amplification means that meet the actual situation in a portable environment.

[0023] The present invention supports the automatic rotation of the gravity-driven microfluidic chip after sample amplification is completed to perform detection, thereby saving manpower and material resources.

[0024] Thermal insulation materials are attached around the thermal conductive material slot to reduce instrument power consumption and increase heating rate; it also supports multiple mode switching and supports independent detection and heating.

[0025] In summary, the present invention is directed to a gravity-driven microfluidic chip based on nucleic acid amplification technology, supports temperature control, realizes automation and integration of nucleic acid amplification and detection, has a small system size and is easy to carry, and has a fast detection speed, which meets the requirements of rapid on-site nucleic acid detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention.

[0027] Figure 2 It is a schematic diagram of an exploded view of a chip plug-in platform structure in an embodiment of the present invention.

[0028] Figure 3 Schematic diagram of the heat conducting material structure in an embodiment of the present invention.

[0029] Figure 4It is a schematic diagram of the structure of the rotating mechanism carrier frame in an embodiment of the present invention.

[0030] Figure 5 It is a schematic diagram of the structure of the rotating mechanism in an embodiment of the present invention.

[0031] Figure 6 It is a framework diagram of the present invention.

[0032] Figure 7 It is a framework diagram of the main control module of the present invention.

[0033] The reference numerals are described as follows:

[0034] 1. Chip direct insertion, 11. Chip direct insertion slot, 12. Thermal conductive material installation slot, 13. Temperature detection module installation hole, 14. Thermal conductive material fixing hole, 15. Rotating mechanism fixing hole row, 16. Thermal insulation material, 17. Heating element;

[0035] 2. Thermal conductive material, 21. Chip wrapping groove (thermal conductive material part), 22. Temperature detection module mounting hole (thermal conductive material part), 23. Thermal conductive material fixing hole (thermal conductive material part);

[0036] 3. Carrier frame, 31. Rotating mechanism mounting slot, 32. Carrier frame mounting hole;

[0037] 4. Rotating mechanism, 41. Rotating arm 42. Rotating mechanism mounting hole (rotating mechanism part). DETAILED DESCRIPTION

[0038] The following is a further description of the specific implementation of the present invention in conjunction with the accompanying drawings. The following implementation is only used to illustrate the present invention, but not to limit the present invention.

[0039] like Figure 6 As shown, the present invention includes a main control module, a heating module, a chip plug-in platform and a rotating module; the main control module is electrically connected to the heating module and the rotating module respectively, the heating module is arranged in the chip plug-in platform, and the chip plug-in platform is installed on the rotating module; in a specific implementation, in the main control module, the main control chip uses a single-chip microcomputer module, and the display part uses a 3-digit digital tube; the heating module uses a U-shaped aluminum block as a heat-conducting material; the rotating mechanism in the rotating module uses a servo module.

[0040] The heating module includes a thermal conductive material 2, a heating element 17 and a temperature detection module. The thermal conductive material 2 is arranged in the chip insertion platform 1. The heating element 17 and the temperature detection module are respectively arranged on the side of the thermal conductive material and in the installation hole of the temperature detection module. The heating element 17 and the temperature detection module are electrically connected to the main control module.

[0041] The heat conducting material 16 is used for heat transfer, and any heat conducting material may be selected, but is not limited thereto.

[0042] The temperature detection module supports real-time temperature detection, display and feedback.

[0043] like Figure 7 As shown, the main control module includes a main control chip, a display module, a keyboard module, a power module and a relay module; the main control chip is electrically connected to the display module, the keyboard module and the relay module respectively, the display module is electrically connected to the keyboard module, the power module is electrically connected to the main control chip, the display module, the keyboard module and the relay module, and the relay module is electrically connected to the heating element 17.

[0044] The main control chip is a single chip microcomputer, but not limited to this;

[0045] The display module can use a 3-digit digital tube, or an OLED screen, etc.

[0046] The keyboard module includes multiple buttons that can control functions such as heating switch, temperature control, mode switching, and chip rotation. It can control the instrument to work in multiple modes as needed, and can support various modules to work together or independently according to actual conditions;

[0047] The power module includes a transformer, a switch, an indicator light, a power supply port, etc.;

[0048] The relay module is connected to the heating element 17 under the control of the main control chip to control the on and off of the heating element circuit.

[0049] like Figure 2 As shown, the chip insertion platform 1 is provided with supports on the side and bottom, which ensure that the chip has mechanical support in both horizontal and vertical states; a chip insertion slot 11 for inserting the microfluidic chip and a heat-conducting material installation slot 12 for loading the heat-conducting material in the heating module are provided;

[0050] A chip slot 11 is provided on one side of the bottom, a microfluidic chip is inserted in the chip slot 11, a heat conductive material installation slot 12 is provided in the middle of the bottom, a heat conductive material of a heating module is arranged inside the heat conductive material installation slot 12, a heat insulating material 16 is provided around the heat conductive material installation slot 12, one side of the bottom of the chip slot 11 is connected with the heat conductive material installation slot 12, the front side of the bottom of the microfluidic chip extends into the heat conductive material installation slot 12 and is in full contact and connection with the heat conductive material in the heat conductive material installation slot 12;

[0051] The front side of the heat-conducting material installation groove 12 is provided with a straight-insert platform fixing hole 14 for fixing the heat-conducting material, and the heat-conducting material is provided with a mounting hole 23 for fixing the heat-conducting material and a temperature detection module mounting hole 22;

[0052] The outer side wall of the chip insertion platform 1 is provided with a rotation mechanism fixing row of holes 15 for connecting with the rotation mechanism 4 .

[0053] The outer side wall of the bottom of the chip insertion platform 1 has a row of screw holes for mechanically connecting with the rotating arm 41 of the steering gear module in the rotating mechanism 4 .

[0054] That is, the rotating mechanism fixing row holes 15 are screw holes, and the screws pass through the through holes on the rotating arm 41 and are threadedly connected to the screw holes of the rotating mechanism fixing row holes 15 .

[0055] The specific chip plug-in platform structure is as follows Figure 1 As shown, slot 11 is used for direct plug-in fixing of the chip, the thermal conductive material is placed in slot 12, and the thermal insulating material 16 is wrapped around it. The thermal conductive material is fixed through screw holes 14; a probe-type NTC thermistor is selected to form a temperature detection module, which is placed in hole 13 for fixing, and the entire direct plug-in platform is mechanically connected to the rotating structure through a row of holes 15. The specific structure of the selected thermal conductive material is as follows Figure 3 As shown, the groove 21 is used to embed the chip and perform double-sided heating.

[0056] like Figure 3 As shown, the thermal conductive material 2 is made into a U shape and placed in the thermal conductive material installation groove 12, and is wrapped with a thermal insulation material 16 on the outside. The U-shaped opening of the thermal conductive material 2 serves as a chip wrapping groove 21, and the chip wrapping groove 21 is connected and docked with the chip straight slot 11. The front side of the bottom of the microfluidic chip is inserted into the chip wrapping groove 21 and is in contact with the thermal conductive material 2. Sensor mounting holes 22 and thermal conductive material fixing holes 23 are respectively provided on both sides of the U shape of the thermal conductive material 2. The sensor mounting hole 22 is used to install the temperature detection module of the heating module, and the thermal conductive material fixing hole 23 is a screw hole for installing bolts to fix the thermal conductive material 2 in the thermal conductive material installation groove 12.

[0057] In a specific implementation, the bolts / screws pass through the plug-in platform fixing holes 14 and the heat-conducting material fixing holes 23 , thereby fixing the U-shaped heat-conducting material 2 in the heat-conducting material installation groove 12 .

[0058] The temperature detection module passes through the plug-in platform installation hole 13 and the temperature detection module installation hole 22 , and is then embedded and loaded inside the thermal conductive material 2 .

[0059] The heating element 17 is placed in the chip packaging groove 21 and attached to the side of the thermal conductive material 2 .

[0060] The rotating module includes a rotating mechanism 4 and a carrier frame 3; the rotating mechanism 4 is mounted on the carrier frame 3, the rotating mechanism 4 is electrically connected to the main control module, and the rotating part of the rotating mechanism 4 is fixedly connected to the chip insertion platform 1 by screws.

[0061] The support frame 3 is used to support the rotating mechanism 4, and the two are also fixed by screws.

[0062] The rotating mechanism supports manual rotation, timed rotation and other forms, and can realize automatic operation.

[0063] like Figure 4 As shown, a rotating mechanism installation groove 31 is provided on the carrier 3, and a rotating mechanism 4 is installed in the rotating mechanism installation groove 31. A strip groove is provided at the bottom of the rotating mechanism installation groove 31, and a carrier installation hole 32 which is connected to the outside and used for installing the rotating mechanism 4 is provided on the groove wall of the strip groove;

[0064] like Figure 5 As shown, the rotating mechanism 4 includes a steering gear module, the output shaft of the steering gear module is fixedly connected to a rotating arm 41, a strip-shaped protrusion is provided at the bottom of the steering gear module, and the strip-shaped protrusion is provided with a rotating mechanism mounting hole 42 for mounting the steering gear module on the carrier 3;

[0065] The steering gear module is installed in the rotating mechanism installation groove 31, and the strip protrusion is inserted into the strip groove. The steering gear module is fixedly installed on the supporting frame 3 by passing the bolts through the rotating mechanism installation hole 42 and the supporting frame installation hole 32.

[0066] The specific rotation module structure is as follows: Figure 4 , Figure 5 As shown, Figure 4 In the figure, the rotating mechanism is placed in the groove 31, fixed by the screw hole 32, and the whole is placed on the flat table surface; Figure 5 It is a structural schematic diagram of the selected rotating mechanism, which is mechanically connected to the plug-in table through the rotating arm 41 and performs rotating work.

[0067] The implementation process of the present invention is as follows:

[0068] Insert the microfluidic chip loaded with reagents into the chip insertion slot 11 of the chip insertion platform 1 and the chip wrapping slot 21 of the thermal conductive material 2, and control the rotating module through the main control module to drive the microfluidic chip insertion platform 1 to be on a flat horizontal surface.

[0069] When the front part of the microfluidic chip needs to be heated for nucleic acid amplification, the main control module controls the heating element 17 in the heating module to heat the heat-conducting material 2, and the heat-conducting material 2 transfers the heat to the front part of the microfluidic chip. During the heating process, the main control module controls the temperature detection module in the heating module to monitor the temperature of the heat-conducting material 2 and the environment in real time.

[0070] After a period of time, the main control module controls the rotating module to drive the microfluidic chip plug-in platform 1 to rotate 90 degrees, so that the amplified liquid in the microfluidic chip flows into the detection chamber containing the test strip inside the chip by gravity for test strip detection.

[0071] During the whole process, the main control module can control the temperature detection module in the heating module to perform real-time temperature detection on the heat-conducting material 2 and the environment.

[0072] The implementation process of the present invention is as follows:

[0073] Figure 2 In the structure, firstly insert the microfluidic chip loaded with reagents into the chip insertion slot 11 and the chip wrapping slot 21 of the chip insertion station, place the entire device on a flat horizontal surface, and then connect the device to a power source.

[0074] Turn on the sliding switch on the main control module panel, and the device starts to power on. First, the rotating mechanism 4 will be initialized, and the chip plug-in table 1 will rotate to a horizontal position. At this time, the device does not heat by default, but the temperature detection module in the heating module will work. The temperature detection module is composed of a detection circuit and an NTC thermistor. When the temperature changes, the resistance of the thermistor will change accordingly. After filtering by the detection circuit and the detection algorithm, the detected voltage value is transmitted to the main control chip, and through its internal AD conversion function, the output of the Celsius temperature value is realized through the corresponding program, so that the digital tube display screen displays the temperature detection data.

[0075] If the heating button of the keyboard module is pressed, the main control module will adjust the algorithm and output high and low level signals to the relay module. The relay module will be energized to drive the heating module to start working. If the stop button of the keyboard module is pressed while the heating module is working, the heating module will stop working.

[0076] The initial target temperature of the device is 65℃ by default. If you need to change the target temperature, press the mode button of the keyboard module to enter the temperature adjustment mode. You can set the target temperature by pressing the heating button and cooling button of the keyboard module. After setting, press the mode button again to switch back to the heating mode.

[0077] When the temperature reaches the target temperature, the temperature detection module transmits data to the main control chip, and the main control chip will compare and adjust through the algorithm to disconnect the relay module, stop the heating module from working, and reduce the temperature; the reflow temperature defaults to 1°C, that is, when the temperature drops to the target temperature minus 1°C, the relay module re-engages and continues to heat, so that the temperature is maintained near the target temperature. For example, when the target temperature is set to 65°C, when the heating module is turned off and the temperature drops to 64°C, the relay module re-engages and continues to heat.

[0078] Figure 5In the structure, the main control board is electrically connected to the rotating mechanism module. When the rotating mechanism 4 needs to rotate, the main control chip will output a PWM signal to the rotating mechanism 4 to control the rotation of the rotating mechanism and the rotation angle. After the heating starts, the rotating part of the rotating mechanism module starts to rotate 90 degrees by default after 30 minutes, so that the liquid amplified in the gravity-driven microfluidic chip flows into the detection chamber containing the test strip for test strip detection. In addition, if the user believes that the preset heating time is not necessary and the reaction is sufficient during the heating process, as shown in the figure, the rotation button of the keyboard module on the main control board can be pressed to rotate the chip in advance and perform the test. After the overall detection is completed, if the chip plug-in station 1 is still in the rotated state at this time, the chip plug-in station 1 can also be reset to a horizontal state by pressing the rotation button of the keyboard module.

[0079] All default values ​​in the present invention can be personalized according to the actual needs of the user by changing the parameters in the burning program.

Claims

1. A portable chip-insertion nucleic acid amplification detection instrument, characterized in that: It includes a main control module, a heating module, a chip plug-in platform and a rotating module; the main control module is electrically connected to the heating module and the rotating module respectively, the heating module is arranged in the chip plug-in platform, and the chip plug-in platform is installed on the rotating module; The heating module comprises a heat-conducting material (2), a heating element (17) and a temperature detection module; the heat-conducting material (2) is arranged in the chip plug-in platform (1); the heating element (17) is located on the side of the chip plug-in platform (1); the temperature detection module is installed in the heat-conducting material (2); and the heating element (17) and the temperature detection module are electrically connected to a main control module.

2. A portable chip-insertion nucleic acid amplification detection instrument according to claim 1, characterized in that: The main control module comprises a main control chip, a display module, a keyboard module, a power module and a relay module; the main control chip is electrically connected to the display module, the keyboard module and the relay module respectively, the display module is electrically connected to the keyboard module, the power module is electrically connected to the main control chip, the display module, the keyboard module and the relay module, and the relay module is electrically connected to the heating element (17).

3. A portable chip-insertion nucleic acid amplification detection instrument according to claim 1, characterized in that: The chip insertion platform (1) is provided with a chip insertion slot (11) and a heat-conducting material installation slot (12); A chip slot (11) is provided at one side of the bottom, a microfluidic chip is inserted in the chip slot (11), a heat conductive material installation groove (12) is provided at the middle position of the bottom, a heat conductive material (2) of a heating module is arranged inside the heat conductive material installation groove (12), a heat insulating material (16) is provided around the slot of the heat conductive material installation groove (12), the chip slot (11) and the heat conductive material installation groove (12) are connected, the front side of the bottom of the microfluidic chip extends into the heat conductive material installation groove (12) and is in contact with and connected to the heat conductive material (2) in the heat conductive material installation groove (12); a heating element (17) is provided on the side of the heat conductive material installation groove (12), and a slot fixing hole (14) for fixing the heat conductive material (2) and a slot fixing hole (13) for installing a temperature detection module in the heating module are provided on the front side of the heat conductive material installation groove (12); and a rotating mechanism fixing row of holes (15) for connecting to the rotating mechanism (4) are provided on the outer side wall of the chip slot (1).

4. A portable chip-insertion nucleic acid amplification detection instrument according to claim 1, characterized in that: The heat conductive material (2) is formed into a U shape and is placed in a heat conductive material installation groove (12); the opening of the U shape of the heat conductive material (2) serves as a chip wrapping groove (21); the front side of the bottom of the microfluidic chip is inserted into the chip wrapping groove (21) and is in contact with the heat conductive material (2); sensor installation holes (22) and heat conductive material fixing holes (23) are respectively provided on both sides of the U shape of the heat conductive material (2); the sensor installation hole (22) is used to install a temperature detection module of a heating module; the heat conductive material fixing hole (23) is a screw hole used to install a bolt to fix the heat conductive material (2) in the heat conductive material installation groove (12).

5. A portable chip-insertion nucleic acid amplification detection instrument according to claim 1, characterized in that: The rotating module comprises a rotating mechanism (4) and a carrier frame (3); the rotating mechanism (4) is mounted on the carrier frame (3), the rotating mechanism (4) is electrically connected to the main control module, and the rotating part of the rotating mechanism (4) is fixedly connected to the chip plug-in platform (1) via screws.

6. A portable chip-insertion nucleic acid amplification detection instrument according to claim 5, characterized in that: The carrier frame (3) is provided with a rotating mechanism installation groove (31), the rotating mechanism (4) is installed in the rotating mechanism installation groove (31), a strip groove is provided at the bottom of the rotating mechanism installation groove (31), and a carrier frame installation hole (32) for installing the rotating mechanism (4) on the groove wall of the strip groove is provided; The rotating mechanism (4) comprises a steering gear module, the output shaft of the steering gear module is fixedly connected to a rotating arm (41), a strip-shaped protrusion is provided at the bottom of the steering gear module, and the strip-shaped protrusion is provided with a rotating mechanism mounting hole (42) for mounting the steering gear module on the carrier (3); The steering gear module is installed in the rotating mechanism installation groove (31), the strip-shaped protrusion is inserted into the strip-shaped groove, and the steering gear module is fixedly installed on the supporting frame (3) by passing bolts through the rotating mechanism installation hole (42) and the supporting frame installation hole (32).

7. A chip-based direct-insertion nucleic acid amplification detection method according to any one of claims 1 to 6, characterized in that: The microfluidic chip loaded with reagents is inserted into the chip insertion slot (11) of the chip insertion platform (1) and the chip wrapping slot (21) of the heat conductive material (2), and the main control module controls the rotating module to drive the microfluidic chip insertion platform (1) to be on a horizontal plane. When the front part of the microfluidic chip needs to be heated for nucleic acid amplification, the main control module controls the heating element (17) in the heating module to heat the heat-conducting material (2), and the heat-conducting material (2) transfers heat to the front part of the microfluidic chip. During the heating process, the main control module controls the temperature detection module in the heating module to monitor the temperature of the heat-conducting material (2) and the environment in real time. After a period of time, the main control module controls the rotating module to drive the microfluidic chip insertion platform (1) to rotate 90 degrees, so that the amplified liquid in the microfluidic chip flows into the detection chamber containing the test strip inside the chip under gravity for test strip detection.

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