Scenedesmus F1, application of scenedesmus F1, scenedesmus solution, culture method and application of scenedesmus solution, and treatment method of nitrogen-containing wastewater

Abstract

The invention belongs to the technical field of biological wastewater treatment, and particularly relates to scenedesmus F1 and application thereof, scenedesmus liquid and a culture method and application thereof, and a treatment method of nitrogen-containing wastewater. The invention provides a scenedesmus sp. F1, and the preservation number of the scenedesmus sp. F1 is CCTCC (China Center For Type Culture Collection) NO: M20242540. The scenedesmus F1 provided by the invention can digest and remove nitrogen in the nitrogen-containing wastewater, and has the advantages of rapid growth and relatively strong adaptability under the condition of relatively high nutrient concentration, so that efficient removal of nitrogen in the nitrogen-containing wastewater is realized; in addition, the scenedesmus F1 also has the advantage of rapid sedimentation, can reduce the energy consumption in the biomass collection process, reduces the overall treatment cost, and has wide application prospects in industrial wastewater treatment.

Description

Technical Field

[0001] The present invention relates to the technical field of biological wastewater treatment, and in particular to a Scenedesmus F1 and an application thereof, a Scenedesmus algae liquid and a cultivation method and an application thereof, and a method for treating nitrogen-containing wastewater. Background Art

[0002] The application of microalgae in wastewater treatment has attracted increasing attention, especially some algae strains that can effectively remove nutrients from wastewater. In the treatment of high-nitrogen wastewater, traditional physical and chemical methods have the problems of high cost and low efficiency, and biological treatment using specific algae strains has become a viable alternative. Summary of the invention

[0003] In view of this, the purpose of the present invention is to provide a Scenedesmus F1 and its application, Scenedesmus algae liquid and its cultivation method and application, and a method for treating nitrogen-containing wastewater. The Scenedesmus F1 provided by the present invention can efficiently remove nitrogen from nitrogen-containing wastewater, making the treatment cost of nitrogen-containing wastewater low.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The invention provides a Desmodesmus sp. F1, and the preservation number of the Desmodesmus sp. F1 is CCTCCNO: M20242540.

[0006] The present invention also provides a Scenedesmus algae liquid, wherein the Scenedesmus algae liquid includes the Scenedesmus F1 described in the above technical solution.

[0007] The present invention also provides a method for culturing the Scenedesmus algae liquid described in the above technical solution, comprising the following steps:

[0008] The Scenedesmus F1 described in the above technical solution is placed on BG11 culture medium and cultured under light conditions.

[0009] Preferably, the intensity of the illumination is 50 to 200 μmolphotons·m - 2 s - 1.

[0010] Preferably, the culture temperature is 20-30°C.

[0011] The present invention also provides the use of the Scenedesmus F1 described in the above technical solution or the Scenedesmus liquid described in the above technical solution in the treatment of nitrogen-containing wastewater.

[0012] The present invention also provides a method for treating nitrogen-containing wastewater, comprising the following steps:

[0013] Scenedesmus F1 was placed in nitrogen-containing wastewater for water treatment;

[0014] The Scenedesmus F1 comes from the Scenedesmus F1 described in the above technical solution or the Scenedesmus liquid described in the above technical solution.

[0015] Preferably, the OD of the Scenedesmus F1 in nitrogen-containing wastewater is 680 It is 0.1~0.5.

[0016] Preferably, the total nitrogen content in the nitrogen-containing wastewater is 20 to 60 mg / mL.

[0017] Preferably, the pH value of the nitrogen-containing wastewater is 7 to 7.5.

[0018] The invention provides a Desmodesmus sp. F1, and the preservation number of the Desmodesmus sp. F1 is CCTCCNO: M20242540.

[0019] The Scenedesmus F1 provided by the present invention can digest and remove nitrogen in nitrogen-containing wastewater, and has the advantages of rapid growth and strong adaptability under conditions of high nutrient concentration, thereby achieving efficient removal of nitrogen in nitrogen-containing wastewater; in addition, Scenedesmus F1 also has the advantage of rapid sedimentation, which can reduce energy consumption in the biomass collection process and reduce the overall treatment cost, so that its application in industrial wastewater treatment has broad prospects.

[0020] Biological Deposit Description

[0021] The F1 strain of Desmodesmus, with the Latin name Desmodesmus sp., was deposited in the China Center for Type Culture Collection (CCTCC) on November 12, 2024. The deposit address is No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, China Center for Type Culture Collection, Wuhan University, and the deposit number is CCTCC NO: M20242540. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 is the growth curve of Scenedesmus F1 in synthetic municipal wastewater;

[0023] Figure 2 is the removal efficiency of total nitrogen (TN) by Scenedesmus F1 in wastewater treatment;

[0024] Figure 3 is the removal efficiency of chemical oxygen demand (COD) by Scenedesmus F1 in wastewater treatment;

[0025] Figure 4 This is a graph of the sedimentation behavior of Scenedesmus F1. DETAILED DESCRIPTION

[0026] The invention provides a Desmodesmus sp. F1, and the preservation number of the Desmodesmus sp. F1 is CCTCCNO: M20242540.

[0027] In the present invention, the Scenedesmus F1 was isolated from a fecal water sample of a pond in Hubei.

[0028] The present invention also provides a Scenedesmus algae liquid, wherein the Scenedesmus algae liquid includes the Scenedesmus F1 described in the above technical solution.

[0029] The present invention also provides a method for culturing the Scenedesmus algae liquid described in the above technical solution, comprising the following steps:

[0030] The Scenedesmus F1 described in the above technical solution is placed on BG11 culture medium and cultured under light conditions.

[0031] In the present invention, the BG11 culture medium is in liquid form.

[0032] In the present invention, the intensity of the light is preferably 50 to 200 μmolphotons·m - 2 s - 1, more preferably 100 to 150 μmolphotons·m - 2 s - 1.

[0033] In the present invention, the culture temperature is preferably 20 to 30°C, more preferably 25°C.

[0034] The present invention also provides the use of the Scenedesmus F1 described in the above technical solution or the Scenedesmus liquid described in the above technical solution in the treatment of nitrogen-containing wastewater.

[0035] The present invention also provides a method for treating nitrogen-containing wastewater, comprising the following steps:

[0036] Scenedesmus F1 was placed in nitrogen-containing wastewater for water treatment;

[0037] The Scenedesmus F1 comes from the Scenedesmus F1 described in the above technical solution or the Scenedesmus liquid described in the above technical solution.

[0038] In the present invention, the OD of the Scenedesmus F1 in nitrogen-containing wastewater is 680 It is preferably 0.1 to 0.5, more preferably 0.22, and specifically may be 0.1, 0.15, 0.2, 0.22, 0.25, 0.3, 0.35, 0.4, 0.45 or 0.5.

[0039] In the present invention, the total nitrogen content in the nitrogen-containing wastewater is preferably 20 to 60 mg / mL.

[0040] In the present invention, the pH value of the nitrogen-containing wastewater is preferably 7 to 7.5, and more preferably 7.2.

[0041] The following detailed description is made of the Scenedesmus F1 and its application, Scenedesmus liquid and its culture method and application, and the method for treating nitrogen-containing wastewater provided by the present invention in conjunction with the embodiments. However, they should not be construed as limiting the scope of protection of the present invention.

[0042] Example 1 Screening and cultivation of algae strain F1

[0043] The samples were collected and separated from a fecal water sample from a pond in Hubei, China. The separation process used the dilution plate method, which was as follows: the samples were diluted 5, 10, and 20 times, and then streaked on BG11 solid medium (agar content of 1.5%) plates, and then placed in a light incubator for culture (light intensity of 50 μmol photons·m - 2 s - 1, temperature 25°C), after a single algae colony grows on the solid plate, pick a single algae colony for streaking separation and culture again, and repeat streaking 3 times to obtain a pure algae strain.

[0044] The purified algae were inoculated in BG11 liquid medium and cultured under a light intensity of 100 μmol photons·m - 2 s - 1. Culture in a light incubator at 25°C.

[0045] Example 2 Identification method of algae strain F1

[0046] The algae strain was identified by 18S rRNA gene sequencing. Genomic DNA was extracted, and the 18S rRNA gene was amplified using 18S forward primer 18SF (5′-CCAACCTGGTTGATCCTGCCAGTA-3′, SEQ ID NO.1) and 18S reverse primer 18SR (5′-CCTTGTTAACGACTTCACCTTCCTCT-3′, SEQ ID NO.2). The amplified product was purified and sequenced by Shanghai Sangon Biotechnology Co., Ltd. The nucleotide sequence of the 18S rRNA gene of the algae strain F1 is shown in SEQID NO.3, as follows:

[0047]

[0048] Then, BLASTn was used to compare the sequence homology with the NCBI database. The results showed that the similarity between algae strain F1 and Desmodesmus sp. was 99.73%, the highest homology, so the algae strain F1 was judged to be Desmodesmus sp.. Desmodesmus F1 was deposited in the China Center for Type Culture Collection of Wuhan University, with the deposit number CCTCC NO: M20242540.

[0049] Example 3 Application of algae strain F1 in treatment of nitrogen-containing wastewater

[0050] The synthetic municipal wastewater was prepared, and its composition was as follows: 200.0 mg / L glucose, 200.0 mg / L sucrose, 68.3 mg / L peptone, 66.7 mg / L ammonium sulfate, 10.9 mg / L ammonium chloride, 4.44 mg / L potassium dihydrogen phosphate, 9.0 mg / L potassium hydrogen phosphate, 21.0 mg / L magnesium sulfate heptahydrate, 2.7 mg / L manganese (II) sulfate heptahydrate, 30.00 mg / L sodium bicarbonate, 19.74 mg / L calcium chloride hexahydrate, and 0.14 mg / L iron (III) chloride hexahydrate. The initial pH of the synthetic municipal wastewater was 7.2, and the initial OD of Scenedesmus F1 in the synthetic municipal wastewater was 2.3. 680 The OD of Scenedesmus F1 was 0.22 after it was treated in synthetic municipal wastewater for different time periods. 680 , the results are as follows Figure 1 As shown, Figure 1 is the growth curve of Scenedesmus F1 in synthetic municipal wastewater. Figure 1 It can be seen that Scenedesmus F1 grows rapidly in wastewater. Compared with the initial inoculation density, the cell density has increased by more than 3 times in 24 hours, and the cell density has reached more than 7 times in 60 hours, and has reached a stable growth period.

[0051] After the treatment of Scenedesmus F1 in synthetic municipal wastewater for different time periods (12, 24, 48 and 72 h), the treated synthetic municipal wastewater was centrifuged at 8000 rpm for 10 min and filtered through a 0.45 μm nylon filter membrane. The concentrations of total nitrogen (TN) and chemical oxygen demand (COD) in the treated synthetic municipal wastewater were measured. The determination method is shown in the national standard scheme: "Water and Wastewater Monitoring and Analysis Methods", Fourth Edition, State Environmental Protection Administration, 2002. The results are as follows Figure 2 and Figure 3 As shown, Figure 2 is the removal efficiency of total nitrogen (TN) by Scenedesmus F1 in wastewater treatment, Figure 3 is the removal efficiency of chemical oxygen demand (COD) by Scenedesmus F1 in wastewater treatment. Figure 2 and Figure 3It can be seen that Scenedesmus F1 can remove 60% of the total nitrogen (TN) in 12 hours, more than 90% of the total nitrogen (TN) after 24 hours, and most of the total nitrogen (TN) within 72 hours; Scenedesmus F1 has a significant removal ability for chemical oxygen demand (COD), removing more than 90% of the chemical oxygen demand (COD) after 72 hours, indicating its high efficiency in treating organic pollutants.

[0052] After Scenedesmus F1 was treated in the synthetic municipal wastewater for 72 hours, 10 mL of the treated synthetic municipal wastewater was gently mixed and transferred to a 10 mL cylindrical glass tube. The mixture was allowed to stand at room temperature for 3 hours, and photographs were taken every hour. The results are shown in Figure 2. Figure 4 As shown, from Figure 4 It can be seen that after standing for 3 hours, most of the algal cells of Scenedesmus F1 have settled to the bottom of the glass tube, and its sedimentation efficiency is more than 60%, indicating its efficient sedimentation performance, which can reduce the energy consumption in the biomass collection process and reduce the overall processing cost.

[0053] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Desmodesmus sp. F1, characterized in that: The deposit number of the Scenedesmus F1 is CCTCC NO: M20242540.

2. A Scenedesmus algae liquid, characterized in that: The Scenedesmus algae liquid includes the Scenedesmus F1 described in claim 1.

3. The method for culturing Scenedesmus algae liquid according to claim 2, characterized in that: The following steps are involved: The Scenedesmus F1 of claim 1 is placed on a BG11 culture medium and cultured under light conditions.

4. The culture method according to claim 3, characterized in that The intensity of the illumination is 50 to 200 μmolphotons·m - 2 s - 1.

5. The culture method according to claim 3, characterized in that: The culture temperature is 20-30°C.

6. Use of the Scenedesmus F1 described in claim 1 or the Scenedesmus liquid described in claim 2 in the treatment of nitrogen-containing wastewater.

7. A method for treating nitrogen-containing wastewater, characterized in that: The following steps are involved: Scenedesmus F1 was placed in nitrogen-containing wastewater for water treatment; The Scenedesmus F1 is derived from the Scenedesmus F1 described in claim 1 or the Scenedesmus liquid described in claim 2.

8. The processing method according to claim 7, characterized in that: The OD of Scenedesmus F1 in nitrogen-containing wastewater 680 It is 0.1~0.

5.

9. The processing method according to claim 7, characterized in that: The total nitrogen content in the nitrogen-containing wastewater is 20 to 60 mg / mL.

10. The processing method according to claim 7 or 9, characterized in that: The pH value of the nitrogen-containing wastewater is 7 to 7.5.

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