Auxiliary tool for measuring content of recombinant collagen

Abstract

The present invention discloses a recombinant collagen content determination auxiliary tool, and relates to the technical field of recombinant collagen content determination, the recombinant collagen content determination auxiliary tool comprises a housing, the lower end surface of the housing is fixedly connected with an anti-skid pad, the surface of the housing is fixedly connected with a water inlet, the upper end surface of the housing is further provided with a start button, and the surface of the housing is fixedly connected with a stabilizing assembly; a heating assembly is further arranged in the shell, the switching assembly is arranged in the shell and used for switching the movement mode of the auxiliary measuring tool, the mixing assembly is arranged in the shell and used for mixing a to-be-detected liquid and a color developing reagent so that the to-be-detected liquid and the color developing reagent can be fully mixed, and the circulating assembly is arranged in the shell and used for circulating the to-be-detected liquid and the color developing reagent. The circulating assembly is used for circularly heating the detection liquid and the liquid of the developing reagent, so that the reaction of the detection liquid and the liquid of the developing reagent is accelerated, and the problems that the detected result obtained by taking out a small amount of to-be-detected solution is possibly inconsistent with the actual result, and when a large amount of to-be-detected solution is taken out, detection personnel cannot well mix the to-be-detected solution and the developing reagent for reaction are solved.

Description

Technical Field

[0001] The invention relates to the technical field of recombinant collagen content determination, in particular to a recombinant collagen content determination auxiliary tool. Background Art

[0002] Collagen is widely distributed in the skin and mucosal tissues of the human body and is firmly bound to epithelial cells to support their biological functions.

[0003] It plays a key role in regulating the function of biological tissues and the interaction between cells and extracellular matrix. Collagen is widely used in the fields of biology, beauty, food and health care, and has great application prospects in the field of medical materials. At present, studies have begun to use collagen as a scaffold material for organ construction. Currently, there are two major sources of collagen, namely animal sources and microbial fermentation. Microbial fermentation is the fastest-growing method for producing collagen. Through genetic engineering technology, various host cells can be selected to produce collagen. The recombinant collagen produced by this method not only has higher hydrophilicity and safety, but also avoids the existence of viral risks, immune rejection and other problems.

[0004] Nowadays, when determining the content of recombinant collagen, a sampling test is usually used. In this test method, a small amount of the test solution needs to be taken out of the bottle and added to the test tube, and then the color reagent is dropped into the test tube, and then the reagent is shaken to mix the two evenly, and then the content of recombinant collagen is judged by the depth of the color change of the test solution in the test tube;

[0005] Although the sampling detection method is representative to a certain extent, due to the precipitation of recombinant collagen when stored in the bottle, the content of recombinant collagen in different positions in the bottle is different when sampling. The result of taking out a small amount of the solution to be tested may not be consistent with the actual situation. When a large amount of the solution to be tested is taken out, the test personnel cannot mix the solution to be tested and the color developing reagent well, which will also affect the measurement result. Therefore, a recombinant collagen content determination auxiliary tool is needed to assist in mixing the solution to be tested and the color developing reagent, so as to determine the recombinant collagen content. Summary of the invention

[0006] The purpose of the present invention is to provide an auxiliary tool for determining the content of recombinant collagen, which solves the problems raised in the above-mentioned background technology.

[0007] To achieve the above object, the present invention provides the following technical solution: a recombinant collagen content determination auxiliary tool, comprising a shell, a non-slip pad is fixedly connected to the lower end surface of the shell, a water inlet is fixedly connected to the surface of the shell, a start button is also provided on the upper end surface of the shell, a stabilizing component is fixedly connected to the surface of the shell, and a heating component is also provided inside the shell. The recombinant collagen content determination auxiliary tool also includes:

[0008] A switching component, the switching component is arranged in the housing, and the switching component is used to switch the movement mode of the measurement auxiliary tool;

[0009] A mixing component, which is disposed in the housing and is used to mix the liquid to be detected and the color developing reagent so that the two are fully mixed;

[0010] A circulation component is arranged in the shell and is used for circulating and heating the liquid of the detection liquid and the color developing reagent, so as to accelerate the reaction between the two.

[0011] Optionally, the stabilizing component includes:

[0012] A fixing ring, the upper end surface of the shell is provided with a through slot, the slot wall of the slot is fixedly connected to the outer surface of the fixing ring, the upper end surface of the fixing ring is rotatably connected to a clamping ring, the upper end surface of the clamping ring is fixedly connected to a beaker rack, and the upper end surface of the clamping ring is eccentrically fixedly connected to the lower end surface of the beaker rack, and the inner wall of the beaker rack is set with a soft rubber material.

[0013] Optionally, the switching component includes:

[0014] The bottom plate, the lower end surface of the bottom plate is fixedly connected to the upper end surface of the anti-slip pad, the upper end surface of the bottom plate is fixedly connected to a limiting sleeve, the inner wall of the limiting sleeve is slidably connected to a switching plate, the lower end surface of the switching plate is fixedly connected to a spring 1, the lower end of the spring 1 is fixedly connected to the upper end surface of the bottom plate, the upper end surface of the limiting sleeve is also fixedly connected to the limiting plate, the lower end surface of the limiting plate abuts against the upper end surface of the switching plate, the upper end surface of the limiting plate is fixedly connected to a fixing frame, the upper end surface side surface of the fixing frame is fixedly connected to a sleeve, a trigger switch is arranged below the switching plate, the lower end surface of the trigger switch is fixedly connected to a spring 2, and the lower end of the spring 2 is fixedly connected to the upper end surface of the bottom plate.

[0015] Optionally, the switching component further includes:

[0016] A trigger tube, the outer surface of the trigger tube is fixedly connected to the inner wall of the sleeve, the lower end surface of the trigger tube is fixedly connected to a spring three, the lower end of the spring three is rotatably connected to a sliding column, the lower end surface of the sliding column is rotatably connected to the upper end surface of the switching plate, and a vertical groove is provided on the surface of the sleeve, the groove wall of the vertical groove is slidably connected to a switching column, a pressure column is provided at the upper end of the switching column, the end surface of the pressure column is fixedly connected to the outer surface of the trigger tube, the lower end side surface of the switching column is fixedly connected to the lower end surface side surface of the sliding column, and the lower end surface of the sleeve is provided with an oblique groove.

[0017] Optionally, the mixing assembly comprises:

[0018] A rotating rod 1, the lower end surface of the rotating rod 1 is rotatably connected to the upper end surface of the trigger tube, the surface of the trigger tube is also fixedly connected to a mounting bracket, the lower end surface of the mounting bracket is fixedly connected to a spring 6, the lower end of the spring 6 is fixedly connected to the upper end surface of the base plate, a motor is provided on the mounting bracket, the output end of the motor is belt-driven with the surface of the rotating rod 1, the surface of the rotating rod 1 is also rotatably connected to a fixed block 1, the outer surface of the fixed block 1 is fixedly connected to a connecting bracket, the upper end of the connecting bracket is fixedly connected to a fixed block 2, and the surface of the connecting bracket is also slidably connected to a return pipe.

[0019] Optionally, the mixing assembly further comprises:

[0020] A one-way disk one, the lower end surface of the one-way disk one is fixedly connected to the upper end surface of the rotating rod one, the tooth surface of the upper end of the one-way disk one is meshingly connected to the one-way disk two, the upper end surface of the one-way disk two is fixedly connected to a spline rod, the surface of the spline rod is slidably connected to a sleeve two, the outer surface of the sleeve two rotates through the lower end surface of the fixed block two, the lower end surface of the fixed block two is fixedly connected to a spring five, the lower end of the spring five is rotatably connected to the upper end surface of the one-way disk two, the upper end surface of the spline rod is fixedly connected to the lower end surface of the beaker rack, and the center of the spline rod is concentric with the center of the clamping ring.

[0021] Optionally, the heating component includes:

[0022] A water tank, wherein the lower end surface of the water tank is fixedly connected to the upper end surface of the inner wall of the shell, a heating rod is fixedly connected to the inner upper end surface of the water tank, an auxiliary water tank is fixedly connected to the side of the water tank, the upper end surface of the auxiliary water tank is fixedly connected to the lower end of the return pipe, and a rotating ring is fixedly connected to the upper end surface of the return pipe, a water trough is provided inside the beaker rack, a notch of the same size as the rotating ring is provided on the lower end surface of the water trough, and the groove wall of the notch is rotatably connected to the surface of the rotating ring.

[0023] Optionally, the circulation component includes:

[0024] A cam, an end face of the cam is provided with a through slot, a slot wall of the slot is fixedly connected to the side face of the lower end of the rotating rod one, a side face of the water tank is fixedly connected to a container, a surface of the container is fixedly connected to a limiting block, an end face of the limiting block is provided with a through slot, a slot wall of the slot is slidably connected to a limiting rod, an end of the limiting rod away from the container is fixedly connected to a pressing plate, the pressing plate is fixedly connected to a spring four toward the end face of the container, an end of the spring four away from the pressing plate is fixedly connected to the end face of the limiting block, the pressing plate is also fixedly connected to a piston toward the end face of the container, the rod end side face of the piston slides through the end face of the container, and the sheet end surface of the piston is fixedly connected to the inner wall of the container;

[0025] One-way valve 1, wherein the inlet end of the one-way valve 1 is fixedly connected to the surface of the container, the outlet end of the one-way valve 1 is fixedly connected to a hose, and the end of the hose away from the one-way valve 1 is fixedly connected to the lower end surface of the rotating ring;

[0026] One-way valve 2, the outlet end of the one-way valve 2 is fixedly connected to the surface of the container, the inlet end of the one-way valve 2 is fixedly connected to a fixed pipe, and one end of the fixed pipe away from the one-way valve 2 is fixedly connected to the side of the water tank.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] 1. The present invention controls whether the beaker rack is shaking or rotating by switching the rotation direction of the motor, and further enables the detection device to adjust the shaking mode of the beaker under different reaction states of the solvent to be detected and the color developing reagent, thereby better promoting the mixing reaction of the solvent to be detected and the color developing reagent, and improving the mixing effect of the determination auxiliary tool on the solvent to be detected and the color developing reagent during the detection process.

[0029] 2. The present invention can control the shaking and rotation of the beaker stand by adjusting the switching component, so that the measurement auxiliary tool can be adjusted according to the states of the solvent to be detected and the color developing reagent during the detection process. When rapid mixing is required, vibration mixing is used, and at this time, the hand is used to press the beaker downward, which can not only make the solvent to be detected and the color developing reagent in the beaker mix faster, but also further ensure the stability of the beaker during mixing. When rapid mixing is not required, the beaker is placed on the beaker stand and then rotated to mix the solutions in the beaker. During rotation, since the bubbles are generated more evenly, the influence of the bubbles on the solvent to be detected and the color developing reagent can be reduced.

[0030] 3. The present invention can further accelerate the reaction speed of the test solution and the color-developing solvent by heating the beaker with hot water, and can improve the uniformity of heating by heating in a water bath manner, thereby ensuring the uniformity of the reaction in the beaker. Then, the continuous heating of the beaker is ensured by the setting of the circulation component, and the heating of the water is also set in the water tank, thereby avoiding the scale generated by long-term heating of water to block the pipeline and affect the circulation effect, and it is also easier to clean the scale. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a front view of the structure of the present invention;

[0032] Figure 2 It is a front view of the internal structure of the housing in the present invention;

[0033] Figure 3 It is a cross-sectional view of the internal structure of the auxiliary water tank in the present invention;

[0034] Figure 4 It is an enlarged view of the structure of the mixing assembly in the present invention;

[0035] Figure 5 It is a front view of the circulation assembly assembly in the present invention;

[0036] Figure 6 It is an enlarged view of the switching component structure in the present invention;

[0037] Figure 7 It is a cross-sectional view of the internal structure of the limiting sleeve in the present invention;

[0038] Figure 8 It is a cross-sectional view of the internal structure of the water tank in the present invention;

[0039] Fig. 9 Find the cross-sectional view of the internal structure of that container for the present invention.

[0040] In the figure: 1. Shell; 2. Beaker holder; 3. Start button; 4. Water inlet; 5. Anti-skid pad; 6. Water tank; 7. Snap ring; 8. Rotating rod 1; 9. Cam; 10. Trigger cylinder; 11. Motor; 12. Mounting frame; 13. Spring 6; 14. Bottom plate; 15. Limit sleeve; 16. Spring 2; 17. Trigger switch; 18. Switching plate; 19. Sink; 20. Fixing ring; 21. Reflux pipe; 22. Auxiliary water tank; 23. Heating rod; 24. Spline rod; 25. Connecting frame; 26. Fixing Block 2; 27, spring 5; 28, fixed block 1; 29, one-way disc 1; 30, one-way disc 2; 31, sleeve 2; 32, hose; 33, container; 34, limit block; 35, spring 4; 36, pressure plate; 37, piston; 38, sleeve; 39, sliding column; 40, switching column; 41, pressure column; 42, limit plate; 43, fixed frame; 44, spring 3; 45, spring 1; 46, rotating ring; 47, one-way valve 1; 48, limit rod; 49, one-way valve 2; 50, fixed pipe. DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] For example, see Figures 1 to 9 The present embodiment provides a recombinant collagen content determination auxiliary tool, including a shell 1, a non-slip pad 5 is fixedly connected to the lower end surface of the shell 1, a water inlet 4 is fixedly connected to the surface of the shell 1, and a start button 3 is also provided on the upper end surface of the shell 1. The switching component is arranged in the shell 1, and the switching component includes:

[0043] The bottom plate 14, the lower end surface of the bottom plate 14 is fixedly connected to the upper end surface of the anti-slip pad 5, the upper end surface of the bottom plate 14 is fixedly connected to the limiting sleeve 15, the inner wall of the limiting sleeve 15 is slidably connected with the switching plate 18, the lower end surface of the switching plate 18 is fixedly connected with a spring 1 45, the lower end of the spring 1 45 is fixedly connected to the upper end surface of the bottom plate 14, the upper end surface of the limiting sleeve 15 is also fixedly connected to the limiting plate 42, the lower end surface of the limiting plate 42 abuts against the upper end surface of the switching plate 18, the upper end surface of the limiting plate 42 is fixedly connected with a fixing frame 43, the upper end surface side surface of the fixing frame 43 is fixedly connected with a sleeve 38, a trigger switch 17 is arranged below the switching plate 18, the lower end surface of the trigger switch 17 is fixedly connected with a spring 2 16, the lower end of the spring 2 16 is fixedly connected to the upper end surface of the bottom plate 14;

[0044] The trigger tube 10, the outer surface of the trigger tube 10 is fixedly connected to the inner wall of the sleeve 38, the lower end surface of the trigger tube 10 is fixedly connected with a spring three 44, the lower end of the spring three 44 is rotatably connected with a sliding column 39, the lower end surface of the sliding column 39 is rotatably connected to the upper end surface of the switching plate 18, and a vertical groove is provided on the surface of the sleeve 38, the groove wall of the vertical groove is slidably connected with a switching column 40, and a pressure column 41 is provided at the upper end of the switching column 40, the end surface of the pressure column 41 is fixedly connected to the outer surface of the trigger tube 10, the lower end side surface of the switching column 40 is fixedly connected to the lower end surface side surface of the sliding column 39, and the lower end surface of the sleeve 38 is provided with an oblique groove.

[0045] In this embodiment, when using the measurement auxiliary tool, after adding the solvent to be detected and the color developing reagent into a beaker in a certain ratio, the beaker is mounted on the beaker rack 2, and the beaker rack 2 is moved downward by pressing the beaker, so that the measurement auxiliary tool has the following two motion states:

[0046] First, when the beaker is pressed to make the beaker rack 2 move downward, the trigger cylinder 10 is also driven to move downward. The trigger cylinder 10 moves downward and moves downward through the pressing column 41, thereby driving the sliding column 39 to move downward. In the process of the sliding column 39 moving downward, the switching plate 18 is pressed downward, so that the switching plate 18 moves downward. The extrusion spring 145 has elastic properties. After the pressing column 41 moves downward to squeeze the switching column 40 to move downward to the inclined groove provided on the lower end surface of the sleeve 38, it will move upward under the elasticity of the spring 145, so that the upper end of the switching column 40 slides on the inclined groove, and the switching column 40 is stuck on the inclined surface of the lower end of the sleeve 38, fixing the switching plate 18 on the lower end surface of the limit sleeve 15, and making the switching plate 18 contact with the trigger switch 17, so that the motor 11 rotates positively, further driving the beaker rack 2 to shake.

[0047] Second, pressing the beaker rack 2 again will drive the sliding column 39 to move downward again through the trigger cylinder 10, so that the switching column 40 will be separated from the inclined surface of the trigger cylinder 10 and rotate again to reach the vertical groove on the lower end surface of the sleeve 38, and then the switching plate 18 will be restored to its original state under the elastic performance of the spring 1 45. At this time, when the measurement auxiliary tool is started again, the motor 11 will rotate in the opposite direction, thereby driving the beaker rack 2 to rotate, and then the solvent to be detected and the color developing reagent in the beaker are mixed;

[0048] The setting of the switching component controls whether the beaker rack 2 is shaking or rotating by switching the rotation direction of the motor 11, and further enables the detection device to adjust the shaking mode of the beaker under different reaction states of the solvent to be detected and the color developing reagent, thereby better promoting the mixing reaction of the solvent to be detected and the color developing reagent, and improving the mixing effect of the detection auxiliary tool on the solvent to be detected and the color developing reagent during the detection process.

[0049] It is worth noting that the present embodiment also includes a mixing component, which is disposed in the housing 1, and the stabilizing component includes:

[0050] The fixing ring 20 has a notch extending therethrough on the upper end surface of the housing 1, the groove wall of the notch is fixedly connected to the outer surface of the fixing ring 20, the upper end surface of the fixing ring 20 is rotatably connected to a clamping ring 7, the upper end surface of the clamping ring 7 is fixedly connected to a beaker rack 2, and the upper end surface of the clamping ring 7 is eccentrically fixedly connected to the lower end surface of the beaker rack 2, and the inner wall of the beaker rack 2 is made of a soft rubber material;

[0051] A rotating rod 8, the lower end surface of the rotating rod 8 is rotatably connected to the upper end surface of the trigger tube 10, the surface of the trigger tube 10 is also fixedly connected to a mounting frame 12, the lower end surface of the mounting frame 12 is fixedly connected to a spring 6 13, the lower end of the spring 6 13 is fixedly connected to the upper end surface of the bottom plate 14, a motor 11 is arranged on the mounting frame 12, the output end of the motor 11 is belt-driven with the surface of the rotating rod 8, the surface of the rotating rod 8 is also rotatably connected to a fixed block 1 28, the outer surface of the fixed block 1 28 is fixedly connected to a connecting frame 25, the upper end of the connecting frame 25 is fixedly connected to a fixed block 2 26, and the surface of the connecting frame 25 is also slidably connected to a return pipe 21;

[0052] One-way disk 1 29, the lower end surface of one-way disk 1 29 is fixedly connected to the upper end surface of rotating rod 1 8, the tooth surface of the upper end of one-way disk 1 29 is meshingly connected with one-way disk 2 30, the upper end surface of one-way disk 2 30 is fixedly connected with spline rod 24, the surface of spline rod 24 is slidably connected with sleeve 2 31, the outer surface of sleeve 2 31 rotates through the lower end surface of fixed block 26, the lower end surface of fixed block 26 is fixedly connected with spring 5 27, the lower end of spring 5 27 is rotatably connected to the upper end surface of one-way disk 2 30, the upper end surface of spline rod 24 is fixedly connected to the lower end surface of beaker rack 2, and the center of the spline rod 24 is concentric with the center of the clamping ring 7.

[0053] In this embodiment, when the motor 11 drives the rotating rod 8 to rotate in different directions, the beaker rack 2 will further have the effect of having different rotation directions:

[0054] First, when the motor 11 drives the rotating rod 1 8 to rotate clockwise, it will further drive the one-way disk 1 29 to rotate clockwise. Since the one-way disk 1 29 cannot mesh with the tooth surface of the one-way disk 2 30 when rotating clockwise, it will push the spline rod 24 to move upward and squeeze the spring 5 27, so that the spline rod 24 reciprocates and drives the beaker rack 2 to move up and down;

[0055] Second, when the motor 11 drives the rotating rod 1 8 to rotate counterclockwise, it will further drive the one-way disc 1 29 to rotate counterclockwise. Since the one-way disc 1 29 will mesh with the tooth surface of the one-way disc 2 30 when rotating counterclockwise, it will drive the spline rod 24 to rotate, and further drive the beaker rack 2 to rotate;

[0056] The setting of the mixing component can control the beaker rack 2 to shake and rotate by adjusting the switching component, so that the measurement auxiliary tool can be adjusted according to the states of the solvent to be detected and the color developing reagent during the detection process. When rapid mixing is required, vibration mixing is used, and at this time, the hand is used to press the beaker downward, which not only allows the solvent to be detected and the color developing reagent in the beaker to be mixed faster, but also can further ensure the stability of the beaker during mixing. When rapid mixing is not required, the beaker is placed on the beaker rack 2, and then the beaker is rotated to mix the solutions in the beaker. During rotation, since the bubbles are generated more evenly, the influence of the bubbles on the solvent to be detected and the color developing reagent can be reduced.

[0057] Embodiment 2, based on the above embodiment:

[0058] See also Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 8 and Fig. 9 In this embodiment, a heating component and a circulation component are further provided inside the shell 1, and the heating component includes a water tank 6, the lower end surface of the water tank 6 is fixedly connected to the upper end surface of the inner wall of the shell 1, and a heating rod 23 is fixedly connected to the inner upper end surface of the water tank 6, and a secondary water tank 22 is fixedly connected to the side of the water tank 6, and the upper end surface of the secondary water tank 22 is fixedly connected to the lower end of the return pipe 21, and a rotating ring 46 is fixedly connected to the upper end surface of the return pipe 21, and a water tank 19 is provided inside the beaker rack 2, and a notch of the same size as the rotating ring 46 is provided on the lower end surface of the water tank 19, and the groove wall of the notch is rotatably connected to the surface of the rotating ring 46;

[0059] The loop components include:

[0060] Cam 9, the end face of cam 9 is provided with a through slot, the slot wall of the slot is fixedly connected to the lower end side face of rotating rod 1, the side face of water tank 6 is fixedly connected to container 33, the surface of container 33 is fixedly connected to limit block 34, the end face of limit block 34 is provided with a through slot, the slot wall of the slot is slidably connected to limit rod 48, the end of limit rod 48 away from container 33 is fixedly connected to pressing plate 36, the pressing plate 36 is fixedly connected to the end face of container 33 with spring four 35, the end of spring four 35 away from pressing plate 36 is fixedly connected to the end face of limit block 34, the pressing plate 36 is also fixedly connected to the end face of container 33 with piston 37, the rod end side face of piston 37 slides through the end face of container 33, and the sheet end surface of piston 37 is fixedly connected to the inner wall of container 33;

[0061] A one-way valve 47, the inlet end of the one-way valve 47 is fixedly connected to the surface of the container 33, the outlet end of the one-way valve 47 is fixedly connected to the hose 32, and the end of the hose 32 away from the one-way valve 47 is fixedly connected to the lower end surface of the rotating ring 46;

[0062] One-way valve 49, the outlet end of one-way valve 49 is fixedly connected to the surface of container 33, the inlet end of one-way valve 49 is fixedly connected to a fixed pipe 50, and the end of fixed pipe 50 away from one-way valve 49 is fixedly connected to the side of water tank 6.

[0063] In this embodiment, the liquid in the water tank 6 is heated by the heating rod 23, and then the motor 11 drives the cam 9 to rotate during the process of driving the rotating rod 8 to rotate. The cam 9 rotates to press the pressing plate 36 and squeeze the spring 4 35, so that the piston 37 can move back and forth in the container 33. At the same time, the one-way flow of the one-way valve 1 47 and the one-way valve 2 49 is cooperated to transport the hot water in the water tank 6 to the water tank 19 for heating the beaker, and a reflux pipe 21 is also provided, so that the water tank 19 and the water tank 6 form a cycle, and further the liquid in the water tank 19 can be exchanged with the liquid in the water tank 6.

[0064] The arrangement of the heating component and the circulation component can further accelerate the reaction speed of the test solution and the color-developing solvent by heating the beaker with hot water, and can improve the uniformity of heating by heating in a water bath manner, thereby ensuring the uniformity of the reaction in the beaker. Then, the continuous heating of the beaker is ensured by the arrangement of the circulation component, and the heating of water is also arranged in the water tank 6, thereby avoiding the scale generated by long-term heating of water to block the pipeline and affect the circulation effect, and it is also easier to clean the scale.

[0065] Working principle: When using the auxiliary tool for determining the content of recombinant collagen, the following steps are involved:

[0066] S1: After adding the solvent to be detected and the color developing reagent into a beaker in a certain proportion, the beaker is mounted on the beaker stand 2;

[0067] S2: Pressing the beaker to move the beaker rack 2, and then determining the movement mode of the beaker rack 2, and then pressing the start button 3 to start the operation of the measurement auxiliary device;

[0068] S3: During operation, when the motor 11 rotates clockwise, it drives the beaker rack 2 to move up and down reciprocatingly, and when the motor 11 rotates counterclockwise, it drives the beaker rack 2 to rotate, thereby utilizing different mixing methods to promote the reaction between the solvent to be detected and the color developing reagent in the beaker;

[0069] S4: During the mixing process, the state of the solvent to be detected and the color developing reagent in the beaker is observed in real time to adjust the movement mode of the beaker rack 2;

[0070] S5: During the mixing process, the heating component and the circulation component will also be driven to continuously transport hot water to the periphery of the beaker to promote the reaction between the solvent to be detected and the color developing reagent in the beaker. After the reaction is complete, the content of the recombinant collagen can be determined by the color of the solution.

[0071] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A recombinant collagen content determination auxiliary tool, comprising a housing (1), characterized in that: The surface of the housing (1) is fixedly connected to a water inlet (4), the upper end surface of the housing (1) is also provided with a start button (3), the surface of the housing (1) is fixedly connected to a stabilizing component, the interior of the housing (1) is also provided with a heating component, and the measurement auxiliary tool further comprises: A switching component, the switching component is arranged in the housing (1) and is used to switch the movement mode of the measurement auxiliary tool; A mixing component, which is arranged in the housing (1) and is used to mix the liquid to be detected and the color developing reagent so that the two are fully mixed; A circulation component is arranged in the housing (1) and is used for circulating and heating the detection liquid and the color developing reagent liquid to accelerate the reaction between the two.

2. The auxiliary tool for determining the content of recombinant collagen according to claim 1, characterized in that: The stabilizing component comprises: A fixing ring (20), the upper end surface of the housing (1) is provided with a through notch, the groove wall of the notch is fixedly connected to the outer surface of the fixing ring (20), the upper end surface of the fixing ring (20) is rotatably connected to a clamping ring (7), the upper end surface of the clamping ring (7) is fixedly connected to a beaker rack (2), and the upper end surface of the clamping ring (7) is eccentrically fixedly connected to the lower end surface of the beaker rack (2), and the inner wall of the beaker rack (2) is made of soft rubber material.

3. The auxiliary tool for determining the content of recombinant collagen according to claim 2, characterized in that: The switching component comprises: The bottom plate (14) is fixedly connected to the lower end surface of the housing (1) with an anti-skid pad (5), the lower end surface of the bottom plate (14) is fixedly connected to the upper end surface of the anti-skid pad (5), the upper end surface of the bottom plate (14) is fixedly connected to a limiting sleeve (15), the inner wall of the limiting sleeve (15) is slidably connected to a switching plate (18), the lower end surface of the switching plate (18) is fixedly connected to a spring (45), the lower end of the spring (45) is fixedly connected to the upper end surface of the bottom plate (14), and the upper end of the limiting sleeve (15) is fixedly connected to the upper end surface of the bottom plate (14). The surface is also fixedly connected to a limit plate (42), the lower end surface of the limit plate (42) is in contact with the upper end surface of the switching plate (18), the upper end surface of the limit plate (42) is fixedly connected to a fixing frame (43), the upper end surface side surface of the fixing frame (43) is fixedly connected to a sleeve (38), a trigger switch (17) is arranged below the switching plate (18), the lower end surface of the trigger switch (17) is fixedly connected to a spring 2 (16), and the lower end of the spring 2 (16) is fixedly connected to the upper end surface of the bottom plate (14).

4. The auxiliary tool for determining the content of recombinant collagen according to claim 3, characterized in that: The switching component also includes: A trigger tube (10), the outer surface of the trigger tube (10) is fixedly connected to the inner wall of the sleeve (38); the lower end surface of the trigger tube (10) is fixedly connected to a spring three (44); the lower end of the spring three (44) is rotatably connected to a sliding column (39); the lower end surface of the sliding column (39) is rotatably connected to the upper end surface of the switching plate (18); a vertical groove is provided on the surface of the sleeve (38); a switching column (40) is slidably connected to the groove wall of the vertical groove; a pressing column (41) is provided at the upper end of the switching column (40); the end surface of the pressing column (41) is fixedly connected to the outer surface of the trigger tube (10); the lower end side surface of the switching column (40) is fixedly connected to the lower end side surface of the sliding column (39); and the lower end surface of the sleeve (38) is provided with an oblique notch.

5. The auxiliary tool for determining the content of recombinant collagen according to claim 4, characterized in that: The mixing assembly comprises: A rotating rod (8), the lower end surface of the rotating rod (8) is rotatably connected to the upper end surface of the trigger tube (10), the surface of the trigger tube (10) is also fixedly connected to a mounting frame (12), the lower end surface of the mounting frame (12) is fixedly connected to a spring (13), the lower end of the spring (13) is fixedly connected to the upper end surface of the bottom plate (14), a motor (11) is arranged on the mounting frame (12), the output end of the motor (11) is belt-driven with the surface of the rotating rod (8), the surface of the rotating rod (8) is also rotatably connected to a fixed block (28), the outer surface of the fixed block (28) is fixedly connected to a connecting frame (25), the upper end of the connecting frame (25) is fixedly connected to a fixed block (26), and the surface of the connecting frame (25) is also slidably connected to a return pipe (21).

6. The auxiliary tool for determining the content of recombinant collagen according to claim 5, characterized in that: The mixing assembly further comprises: A one-way disc (29), the lower end surface of the one-way disc (29) is fixedly connected to the upper end surface of the rotating rod (8), the tooth surface of the upper end of the one-way disc (29) is meshingly connected to the one-way disc (30), the upper end surface of the one-way disc (30) is fixedly connected to a spline rod (24), the surface of the spline rod (24) is slidably connected to a sleeve (31), the outer surface of the sleeve (31) rotatably passes through the lower end surface of the fixed block (26), the lower end surface of the fixed block (26) is fixedly connected to a spring (27), the lower end of the spring (27) is rotatably connected to the upper end surface of the one-way disc (30), the upper end surface of the spline rod (24) is fixedly connected to the lower end surface of the beaker rack (2), and the center of the spline rod (24) is concentric with the center of the clamping ring (7).

7. The auxiliary tool for determining the content of recombinant collagen according to claim 5, characterized in that: The heating assembly comprises: A water tank (6), the lower end surface of the water tank (6) is fixedly connected to the upper end surface of the inner wall of the shell (1), a heating rod (23) is fixedly connected to the inner upper end surface of the water tank (6), a side surface of the water tank (6) is fixedly connected to an auxiliary water tank (22), the upper end surface of the auxiliary water tank (22) is fixedly connected to the lower end of the return pipe (21), and the upper end surface of the return pipe (21) is fixedly connected to a rotating ring (46), a water tank (19) is provided inside the beaker rack (2), a notch of the same size as the rotating ring (46) is provided on the lower end surface of the water tank (19), and the groove wall of the notch is rotatably connected to the surface of the rotating ring (46).

8. The auxiliary tool for determining the content of recombinant collagen according to claim 7, characterized in that: The loop components include: A cam (9), wherein the end surface of the cam (9) is provided with a through slot, the slot wall of the slot is fixedly connected to the lower end side surface of the rotating rod, the side surface of the water tank (6) is fixedly connected to a container (33), the surface of the container (33) is fixedly connected to a limiting block (34), the end surface of the limiting block (34) is provided with a through slot, the slot wall of the slot is slidably connected to a limiting rod (48), and the end of the limiting rod (48) away from the container (33) is fixedly connected to a pressing plate (36), The pressing plate (36) is fixedly connected to a spring four (35) on the end surface facing the container (33); one end of the spring four (35) away from the pressing plate (36) is fixedly connected to the end surface of the limit block (34); the pressing plate (36) is also fixedly connected to a piston (37) on the end surface facing the container (33); the rod end side surface of the piston (37) slides through the end surface of the container (33), and the sheet end surface of the piston (37) is fixedly connected to the inner wall of the container (33); a one-way valve (47), wherein the inlet end of the one-way valve (47) is fixedly connected to the surface of the container (33), the outlet end of the one-way valve (47) is fixedly connected to a hose (32), and the end of the hose (32) away from the one-way valve (47) is fixedly connected to the lower end surface of the rotating ring (46); A second one-way valve (49), the outlet end of the second one-way valve (49) is fixedly connected to the surface of the container (33), the inlet end of the second one-way valve (49) is fixedly connected to a fixed pipe (50), and the end of the fixed pipe (50) away from the second one-way valve (49) is fixedly connected to the side of the water tank (6).

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