Nutrient enrichment method for hatched fairy shrimp nauplii

Abstract

The invention provides a nutrient enrichment method for hatched fairy shrimp nauplii, which specifically comprises the following steps: (1) an initial hatching stage: 1-20 days after the fairy shrimp nauplii are hatched, adjusting the salinity of a culture pond to be 25-35% o, the dissolved oxygen to be 5-7 mg / L and the temperature to be 25-28 DEG C, alternately irradiating by adopting different illumination light A, and feeding nutritional feed A every day; and (2) a later stage: 20-40 days after incubation of fairy shrimp nauplii, adjusting the salinity of the culture pond to be 35-45% o, the dissolved oxygen to be 5-7 mg / L and the temperature to be 28-30 DEG C, injecting water and rolling over every 3-5 days, alternately irradiating by adopting different light B, feeding nutritional feed B every day, and harvesting strengthened fairy shrimps. According to the method, the initial incubation stage and the later stage are set, in each stage, a specific illumination irradiation mode is adopted to be matched with nutritional feed, rapid growth and nutrient enrichment of the incubated fairy shrimp nauplii are promoted, the body length of the enriched fairy shrimps is 10.5-12.7 mm, the crude protein content is 78.4-82.4%, the content of long-chain polyunsaturated fatty acids EPA and DHA is increased, and the yield of the fairy shrimps is increased. The feed is an excellent live bait for aquatic animals.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a method for enhancing the nutrition of hatched Artemia nauplii. Background Art

[0002] Artemia, also known as salt water Artemia, is an important biological bait. Its newly hatched nauplii have been widely used in the shrimp seedling cultivation process, and its post-larvae and adults are also palatable baits for many shrimp seedlings. Nutritional fortification is the process of increasing the nutritional value of Artemia and transferring it to the seedlings to meet the nutritional needs of aquatic seedlings. As a biological bait for aquatic animals, Artemia should be rich in protein, fat and other contents to provide aquatic animals with more balanced nutrients.

[0003] However, the nutrient uptake rate of the hatched Artemia nauplii is low, especially the lack of essential fatty acids (long-chain polyunsaturated fatty acids EPA and DHA). At the same time, the nauplii are weak in size and easily affected by environmental changes, and the reproductive capacity of the enhanced Artemia should be taken into account. Summary of the invention

[0004] In view of this, the present invention provides a method for enhancing nutrition of hatched Artemia nauplii.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A method for nutritional enhancement of hatched Artemia nauplii, comprising the following steps:

[0007] (1) Initial hatching stage: 1-20 days after the Artemia nauplii are hatched, the salinity of the culture pond is adjusted to 25-35‰, the dissolved oxygen is 5-7 mg / L, and the temperature is 25-28°C. During this period, different light A are used alternately, and nutritious feed A is fed every day;

[0008] The nutritional feed A comprises the following raw materials: composite algae powder, mixed polysaccharides, antimicrobial peptides, and composite fatty acids;

[0009] (2) Late stage: 20-40 days after the hatching of Artemia nauplii, the salinity of the culture pond is adjusted to 35-45‰, the dissolved oxygen is 5-7 mg / L, and the temperature is 28-30°C. The pond is filled with water and rolled every 3-5 days. During this period, different light intensity B is used alternately, and nutritious feed B is fed every day to harvest the fortified Artemia.

[0010] The nutritional feed B comprises the following raw materials: plant extracts, composite microbial agents, spirulina powder, and vegetable blending oil.

[0011] Furthermore, the nutritional feed A specifically includes, by weight: 5-10 parts of compound algae powder, 5-10 parts of mixed polysaccharides, 2-5 parts of antimicrobial peptides, and 1-3 parts of compound fatty acids.

[0012] Furthermore, the nutritional feed B specifically includes, by weight: 10-15 parts of plant extract, 4-6 parts of composite microbial agent, 3-5 parts of spirulina powder, and 2-4 parts of vegetable blending oil.

[0013] Furthermore, the mixed polysaccharide comprises β-glucan, astragalus polysaccharide, kudzu root polysaccharide and inulin in a mass ratio of (3-5):(2-4):(1-3):(1-3).

[0014] Furthermore, the composite algae powder is composed of Dunaliella powder, Nannochloropsis pseudochloropsis powder and Pinus spinulosa powder in a mass ratio of (3-7):1:(1-3).

[0015] Furthermore, the complex fatty acid is composed of ω-3 fatty acids and ω-6 fatty acids in a mass ratio of (1-2):(5-10).

[0016] Furthermore, the different light irradiation A is to irradiate with blue light of intensity 1000-1500 lux for 2-4 hours first, and then irradiate with red light of intensity 600-800 lux for 4-6 hours, alternating between the two.

[0017] Furthermore, the composite microbial agent includes Candida utilis, Bacillus subtilis powder, and Bacillus licheniformis powder mixed in a mass ratio of (3-5):(2-3):(1-3).

[0018] Furthermore, the plant extracts are Garcinia cambogia peel extract, Callicarpa nudiflora extract and Eclipta prostrata extract in a mass ratio of (3-5):(1-3):(2-4).

[0019] Furthermore, the different light irradiation B is first irradiating with 800-1000 lux green light for 4-8 hours, and then irradiating with 1500-2000 lux white light for 3-5 hours, alternating between the two.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The invention sets two stages, namely the initial hatching stage and the later stage, and adopts a specific light irradiation mode and the combination of nutritious feed in stages to promote the rapid growth and nutritional enhancement of the hatched Artemia nauplii. The Artemia nauplii after 40 days of cultivation are 10.5-12.7 mm in length, 78.4-82.4% in crude protein content, 13.57% in long-chain polyunsaturated fatty acid EPA content, and 1.94% in DHA content, and are excellent live bait for aquatic animals. In addition, the nutritionally enhanced Artemia nauplii of the invention have strong reproductive capacity, and each tail can lay up to 1020 eggs on average, indicating that the reproductive capacity of the nutritionally enhanced Artemia nauplii of the invention is enhanced, and the breeding cycle of the enhanced Artemia nauplii is shortened. DETAILED DESCRIPTION

[0022] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0023] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0024] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0025] Vegetable blended oil (ingredients: 10% peanut oil, 20% corn oil, 69% soybean oil and 1% sesame oil) was purchased from Shandong Shengfudi Oil Co., Ltd.

[0026] Garcinia cambogia peel extract was purchased from Shaanxi Huike Plant Development Co., Ltd.

[0027] Callicarpa nudiflora extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd.

[0028] Eclipta prostrata extract was purchased from Shaanxi New Horizon Biotechnology Co., Ltd.

[0029] Candida utilis was purchased from Wuhan Qiaofeng Chemical Technology Co., Ltd.

[0030] Bacillus subtilis powder was purchased from Nanjing Bermuda Biotechnology Co., Ltd.

[0031] Bacillus licheniformis powder was purchased from Cangzhou Zhongxin Biotechnology Co., Ltd.

[0032] Dunaliella powder was purchased from Wuhan Fengzhulin Chemical Technology Co., Ltd.

[0033] The spinulosa algae powder was purchased from Ningxia Vanilla Biotechnology Co., Ltd.

[0034] Nannochloropsis was purchased from Bolu (Xiamen) Biological Co., Ltd.

[0035] Example 1

[0036] A method for nutritional enhancement of hatched Artemia nauplii, comprising the following steps:

[0037] (1) Initial hatching stage: 1-20 days after the hatching of Artemia nauplii, the salinity of the culture pond was adjusted to 30‰, the dissolved oxygen to 6 mg / L, and the temperature to 27±1°C. During the initial hatching stage, the nauplii were irradiated with blue light of 1300 lux for 3 h and then with red light of 700 lux for 5 h, alternating irradiation. Nutrient feed A (8 parts of compound algae powder, 8 parts of mixed polysaccharides, 3 parts of antimicrobial peptides, and 2 parts of compound fatty acids) was fed every day.

[0038] The mixed polysaccharide is β-glucan, astragalus polysaccharide, kudzu root polysaccharide and inulin in a mass ratio of 4:3:2:2;

[0039] The composite algae powder is composed of Dunaliella powder, Nannochloropsis powder and Pinellia powder in a mass ratio of 5:1:2;

[0040] The complex fatty acids consist of ω-3 fatty acids and ω-6 fatty acids in a mass ratio of 1:8;

[0041] (2) Late stage: 20-40 days after the hatching of Artemia nauplii, the salinity of the culture pond was adjusted to 40‰, the dissolved oxygen to 6 mg / L, and the temperature to 29±1°C. The pond was filled with water and rolled every 4 days. In the late stage, the pond was irradiated with 900 lux green light for 6 h, followed by 1800 lux white light for 4 h, alternating irradiation. Nutrient feed B (13 parts of plant extract, 5 parts of compound microbial agent, 4 parts of spirulina powder, and 3 parts of plant blending oil) was fed every day to harvest the fortified Artemia.

[0042] The composite microbial agent includes Candida utilis, Bacillus subtilis powder and Bacillus licheniformis powder, which are mixed in a mass ratio of 4:2:1;

[0043] The plant extracts are garcinia cambogia peel extract, callicarpa nudiflora extract and eclipta prostrata extract in a mass ratio of 4:2:3.

[0044] Example 2

[0045] A method for nutritional enhancement of hatched Artemia nauplii, comprising the following steps:

[0046] (1) Initial hatching stage: 1-20 days after the hatching of Artemia nauplii, the salinity of the culture pond was adjusted to 2‰, the dissolved oxygen to 5 mg / L, and the temperature to 27±1°C. During the initial hatching stage, the nauplii were irradiated with blue light of 1000 lux for 2 h, and then with red light of 600 lux for 4 h, alternating irradiation. Nutrient feed A (5 parts of compound algae powder, 5 parts of mixed polysaccharides, 2 parts of antimicrobial peptides, and 1 part of compound fatty acids) was fed every day.

[0047] The mixed polysaccharide is β-glucan, astragalus polysaccharide, kudzu root polysaccharide and inulin in a mass ratio of 3:2:1:1;

[0048] The composite algae powder is composed of Dunaliella powder, Nannochloropsis powder and Pinellia powder in a mass ratio of 3:1:1;

[0049] The complex fatty acids consist of ω-3 fatty acids and ω-6 fatty acids in a mass ratio of 1:5;

[0050] (2) Late stage: 20-40 days after the hatching of Artemia nauplii, the salinity of the culture pond was adjusted to 35‰, the dissolved oxygen to 5 mg / L, and the temperature to 29±1°C. The pond was filled with water and rolled every 3 days. In the late stage, the pond was irradiated with 800 lux green light for 4 h, followed by 1500 lux white light for 3 h, alternating irradiation. Nutrient feed B (10 parts of plant extract, 4 parts of compound microbial agent, 3 parts of spirulina powder, and 2 parts of plant blending oil) was fed every day to harvest the fortified Artemia.

[0051] The composite microbial agent includes Candida utilis, Bacillus subtilis powder and Bacillus licheniformis powder, which are mixed in a mass ratio of 3:2:1;

[0052] The plant extracts are garcinia cambogia peel extract, callicarpa nudiflora extract and eclipta prostrata extract in a mass ratio of 3:1:2.

[0053] Example 3

[0054] A method for nutritional enhancement of hatched Artemia nauplii, comprising the following steps:

[0055] (1) Initial hatching stage: 1-20 days after the hatching of Artemia nauplii, the salinity of the culture pond was adjusted to 35‰, the dissolved oxygen to 7 mg / L, and the temperature to 27±1°C. During the initial hatching stage, the nauplii were irradiated with blue light of 1500 lux for 4 h and then with red light of 800 lux for 6 h, alternating irradiation. Nutrient feed A (10 parts of compound algae powder, 10 parts of mixed polysaccharides, 5 parts of antimicrobial peptides, and 3 parts of compound fatty acids) was fed every day.

[0056] The mixed polysaccharide is β-glucan, astragalus polysaccharide, kudzu root polysaccharide and inulin in a mass ratio of 5:4:3:3;

[0057] The composite algae powder is composed of Dunaliella powder, Nannochloropsis powder and Pinus spinulosa powder in a mass ratio of 7:1:3;

[0058] The complex fatty acids consist of ω-3 fatty acids and ω-6 fatty acids in a mass ratio of 1:10;

[0059] (2) Late stage: 20-40 days after the hatching of Artemia nauplii, the salinity of the culture pond was adjusted to 45‰, the dissolved oxygen to 7 mg / L, and the temperature to 29±1°C. The pond was filled with water and rolled every 5 days. In the late stage, the pond was first irradiated with 1000 lux green light for 8 h, followed by 2000 lux white light for 5 h, alternating irradiation. Nutrient feed B (15 parts of plant extract, 6 parts of compound microbial agent, 5 parts of spirulina powder, and 4 parts of plant blending oil) was fed every day to harvest the fortified Artemia.

[0060] The composite microbial agent includes Candida utilis, Bacillus subtilis powder and Bacillus licheniformis powder, which are mixed in a mass ratio of 5:3:3;

[0061] The plant extracts are garcinia cambogia peel extract, callicarpa nudiflora extract and eclipta prostrata extract in a mass ratio of 5:3:4.

[0062] Comparative Example 1

[0063] The difference from Example 1 is that the amount of raw materials of the nutritious feed A is different, and the rest is the same as Example 1.

[0064] The nutritional feed A of this comparative example specifically includes, by weight: 3 parts of compound algae powder, 4 parts of mixed polysaccharides, 6 parts of antimicrobial peptides, and 4 parts of compound fatty acids.

[0065] Comparative Example 2

[0066] The difference from Example 1 is that the mixed polysaccharides in the nutritional feed A are β-glucan, astragalus polysaccharide, kudzu root polysaccharide and inulin in a mass ratio of 1:2:1:4, and the rest is consistent with Example 1.

[0067] Comparative Example 3

[0068] The difference from Example 1 is that the mixed polysaccharides in the nutritional feed A lack Pueraria polysaccharide, and the missing raw materials are allocated to other components according to the ratio of β-glucan, astragalus polysaccharide and inulin; the rest is consistent with Example 1.

[0069] Comparative Example 4

[0070] The difference from Example 1 is that the composite algae powder in the nutritional feed A is Dunaliella powder, Nannochloropsis pseudochloropsis powder and Pinus spinulosa powder in a mass ratio of 1:1:1; the rest is consistent with Example 1.

[0071] Comparative Example 5

[0072] The difference from Example 1 is that the composite algae powder in the nutritional feed A lacks Nannochloropsis powder, and the missing raw materials are allocated to other components according to the ratio of Dunaliella powder and Pinus spinulosa powder; the rest is consistent with Example 1.

[0073] Comparative Example 6

[0074] The difference from Example 1 is that the complex fatty acid in the nutritional feed A is composed of ω-3 fatty acids and ω-6 fatty acids in a mass ratio of 2:1; the rest is consistent with Example 1.

[0075] Comparative Example 7

[0076] The difference from Example 1 is that the amount of raw materials of nutrient feed B is different, and the rest is the same as Example 1.

[0077] The nutritional feed A in this comparative example specifically comprises, by weight: 8 parts of plant extracts, 7 parts of composite microbial agents, 6 parts of spirulina powder, and 1 part of vegetable blending oil.

[0078] Comparative Example 8

[0079] The difference from Example 1 is that the composite microbial agent in the nutritional feed B is Candida utilis, Bacillus subtilis powder and Bacillus licheniformis powder in a mass ratio of 1:2:1, and the rest is consistent with Example 1.

[0080] Comparative Example 9

[0081] The difference from Example 1 is that the composite microbial agent in the nutritional feed B lacks Candida utilis, and the missing raw materials are allocated to other components according to the ratio of Bacillus subtilis powder and Bacillus licheniformis powder. The rest is consistent with Example 1.

[0082] Comparative Example 10

[0083] The difference from Example 1 is that the plant extracts in the nutritional feed B are Garcinia cambogia peel extract, Callicarpa nudiflora extract and Eclipta prostrata extract in a mass ratio of 1:4:5, and the rest is consistent with Example 1.

[0084] Comparative Example 11

[0085] The difference from Example 1 is that the plant extract in the nutritional feed B lacks Garcinia cambogia peel extract, and the missing raw material is allocated to other components according to the ratio of Callicarpa nudiflora extract and Eclipta prostrata extract. The rest is consistent with Example 1.

[0086] Comparative Example 12

[0087] The difference from Example 1 is that the different light irradiation A is to irradiate with blue light of intensity 700 lux for 3 hours first, and then irradiate with red light of intensity 1300 lux for 5 hours, alternating irradiation, and the rest is the same as Example 1.

[0088] Comparative Example 13

[0089] The difference from Example 1 is that different light irradiation B is to irradiate with 900 lux white light for 6 hours first, and then irradiate with 1800 lux green light for 4 hours, alternating irradiation, and the rest is the same as Example 1.

[0090] Test example

[0091] Test location: Fishing Bay Base, Fengjia Bay, Huiwen Town, Wenchang City, Hainan Province

[0092] Specific method: The culture ponds were divided into Example 1-3 groups and Comparative Example 1-13 groups, and the culture area of ​​each test group was 10×10m 2 , water depth 3m, hatched Artemia nauplii (density 50 / m 2 ), the shrimps were cultured according to the nutritional fortification methods of Examples 1-3 and Comparative Examples 1-13, and the fortified Artemia was harvested after 40 days. The same number of 20 fortified Artemia were randomly selected from the culture ponds of the Example 1-3 group and the Comparative Example 1-13 group, and the body length (body length is the length from the top of the head of the Artemia to the end of the tail) and crude protein content (the crude protein content of the experimental shrimps was determined using a Kjeldahl nitrogen analyzer (FOSS Kjeltec 8400)) of each tail were measured, and the average value was calculated.

[0093] In addition, 10 pairs of mature male and female adults were randomly selected from the breeding ponds of Example 1-3 and Comparative Example 1-13, and cultured in a disinfected breeding pond for 15 days. During this period, they continued to be fed with feed for the enhanced stage of Artemia, and the eggs of Artemia that sank to the bottom were collected by a straw for the determination of fertility, and the average number of eggs laid per fish was calculated.

[0094] The body length, crude protein content and average number of eggs per tail are shown in Table 1.

[0095] Table 1

[0096] sample Body length / mm Crude protein content / % Average number of eggs laid per tail Example 1 12.7 82.4 1020 Example 2 11.8 80.1 980 Example 3 10.5 78.4 967 Comparative Example 1 8.5 65.7 708 Comparative Example 2 7.7 55.4 644 Comparative Example 3 7.4 52.8 623 Comparative Example 4 6.7 47.3 615 Comparative Example 5 6.9 49.1 637 Comparative Example 6 7.0 51.6 655 Comparative Example 7 8.2 66.2 697 Comparative Example 8 6.8 49.5 614 Comparative Example 9 6.4 47.6 589 Comparative Example 10 6.5 49.7 609 Comparative Example 11 6.3 54.1 604 Comparative Example 12 8.4 67.8 687 Comparative Example 13 8.7 68.1 692

[0097] As can be seen from Table 1, compared with Comparative Examples 1-13, the Artemia obtained by the nutritional fortification method of Examples 1-3 of the present invention is set to two stages, namely the initial hatching stage and the late stage, and each stage adopts a specific lighting irradiation method and a combination of nutritious feed. The body length of the fortified Artemia can reach up to 12.7 mm, and the crude protein content is up to 82.4%. The fortified Artemia has strong reproductive capacity, and the average number of eggs laid per tail can reach up to 1020, indicating that the reproductive capacity of the nutritionally fortified Artemia of the present invention is enhanced.

[0098] In addition, Artemia was randomly selected from the culture ponds of Example 1-3 and Comparative Example 1-13, and samples were taken, freeze-dried, and fatty acid determination (EPA and DHA content) was performed. After extracting the total lipid of Artemia by the Bligh-Dyer method, saponification and methyl esterification were performed, and the gas chromatography was measured by Agilent 7890A gas chromatograph according to GB10219-88. The results are shown in Table 2.

[0099] Table 2

[0100] sample EPA Content DHA content Example 1 13.57％ 1.94％ Example 2 12.78％ 1.87％ Example 3 12.44％ 1.79％ Comparative Example 1 7.57％ 1.31％ Comparative Example 2 6.88％ 1.24％ Comparative Example 3 6.51％ 1.17％ Comparative Example 4 5.67％ 1.12％ Comparative Example 5 5.74％ 1.13％ Comparative Example 6 6.01％ 1.16％ Comparative Example 7 7.44％ 1.24％ Comparative Example 8 5.63％ 1.11％ Comparative Example 9 5.41％ 1.03％ Comparative Example 10 5.59％ 1.05％ Comparative Example 11 5.31％ 1.01％ Comparative Example 12 7.53％ 1.32％ Comparative Example 13 7.61％ 1.38％

[0101] As can be seen from Table 2, compared with Comparative Examples 1-13, the essential fatty acids (long-chain polyunsaturated fatty acids EPA and DHA) in Artemia obtained by the nutritional fortification method of Examples 1-3 of the present invention are increased, among which the long-chain polyunsaturated fatty acid EPA content of Example 1 is 13.57%, and the DHA content is 1.94%, which is an excellent live bait for aquatic animals.

[0102] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for nutritional enhancement of hatched Artemia nauplii, characterized in that: The following steps are involved: (1) Initial hatching stage: 1-20 days after the Artemia nauplii are hatched, the salinity of the culture pond is adjusted to 25-35‰, the dissolved oxygen is 5-7 mg / L, and the temperature is 25-28°C. During this period, different light A are used alternately, and nutritious feed A is fed every day; The nutritional feed A comprises the following raw materials: composite algae powder, mixed polysaccharides, antimicrobial peptides, and composite fatty acids; (2) Late stage: 20-40 days after the hatching of Artemia nauplii, the salinity of the culture pond is adjusted to 35-45‰, the dissolved oxygen is 5-7 mg / L, and the temperature is 28-30°C. The pond is filled with water and rolled every 3-5 days. During this period, different light intensity B is used alternately, and nutritious feed B is fed every day to harvest the fortified Artemia. The nutritional feed B comprises the following raw materials: plant extracts, composite microbial agents, spirulina powder, and vegetable blending oil.

2. The method for nutritional enhancement of hatched Artemia nauplii according to claim 1, characterized in that: The nutritional feed A specifically comprises, by weight: 5-10 parts of compound algae powder, 5-10 parts of mixed polysaccharides, 2-5 parts of antimicrobial peptides, and 1-3 parts of compound fatty acids.

3. The method for nutritional enhancement of hatched Artemia nauplii according to claim 1, characterized in that: The nutritional feed B specifically includes, by weight: 10-15 parts of plant extract, 4-6 parts of composite microbial agent, 3-5 parts of spirulina powder, and 2-4 parts of vegetable blending oil.

4. The method for nutritional enhancement of hatched Artemia nauplii according to claim 2, characterized in that: The mixed polysaccharide comprises beta-glucan, astragalus polysaccharide, kudzu root polysaccharide and inulin in a mass ratio of (3-5):(2-4):(1-3):(1-3).

5. The method for nutritional enhancement of hatched Artemia nauplii according to claim 2, characterized in that: The composite algae powder is composed of Dunaliella powder, Nannochloropsis pseudochloropsis powder and Pinellia solani powder in a mass ratio of (3-7):1:(1-3).

6. The method for nutritional enhancement of hatched Artemia nauplii according to claim 2, characterized in that: The complex fatty acid is composed of ω-3 fatty acid and ω-6 fatty acid in a mass ratio of (1-2):(5-10).

7. The method for nutritional enhancement of hatched Artemia nauplii according to claim 1, characterized in that: The different light irradiation A is to irradiate with blue light of intensity 1000-1500 lux for 2-4 hours first, and then irradiate with red light of intensity 600-800 lux for 4-6 hours, alternately.

8. The method for nutritional enhancement of hatched Artemia nauplii according to claim 3, characterized in that: The composite microbial agent comprises Candida utilis, Bacillus subtilis powder and Bacillus licheniformis powder, which are mixed in a mass ratio of (3-5):(2-3):(1-3).

9. The method for nutritional enhancement of hatched Artemia nauplii according to claim 3, characterized in that: The plant extracts are Garcinia cambogia peel extract, Callicarpa nudiflora extract and Eclipta prostrata extract in a mass ratio of (3-5):(1-3):(2-4).

10. The method for nutritional enhancement of hatched Artemia nauplii according to claim 1, characterized in that: The different light irradiation B is to irradiate with green light of 800-1000 lux for 4-8 hours, and then irradiate with white light of 1500-2000 lux for 3-5 hours, alternating between the two.