Pesticide composition for preventing and treating strawberry bacterial wilt

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating strawberry bacterial wilt. The invention relates to a sterilization pesticide composition. The effective components of the sterilization pesticide composition are compounded by copper gluconate and aureonucleomycin or benziothiazolinone. The copper gluconate is compounded with the existing bactericide aureonucleomycin or benziothiazolinone according to a certain mass ratio, the effect of the compounded combination on strawberry bacterial wilt is measured through an indoor turbidity method, and the result shows that the co-toxicity coefficient of the compounded combination on strawberry bacterial wilt bacteria is larger than 120; therefore, the pesticide composition disclosed by the invention has a synergistic effect on strawberry bacterial wilt.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling strawberry bacterial wilt. Background Art

[0002] Pseudomonas solanacarum is a gram-negative bacterium and a soil-dwelling fungus that has invaded plants through wounds on the roots and stems, causing the plants to wilt and die. Plant diseases caused by Pseudomonas solanacarum are major bacterial diseases that can infect hundreds of plants from more than 40 families, including many plants with important economic value, such as ginger, potato, tomato, tobacco, eggplant, strawberry, pepper and banana. Among them, in the early stage of strawberry bacterial wilt, 1-2 leaves wilt and fall off, and the petioles droop like burns, which can recover at night; a few days after the onset of the disease, the whole plant wilts. When the roots are affected, the petioles of the aboveground leaves are purple-red and all die. When the root crown is cut, the vascular bundles in the cross section are browned, and milky bacterial fluid flows out.

[0003] At present, in the strawberry production process, the main prevention and control of strawberry wilt is still spraying chemical agents. However, due to the long-term irrational use of chemical agents, strawberry wilt has developed varying degrees of resistance to the various existing fungicides, resulting in reduced prevention and control effects. In addition, the intensive use of chemical agents has led to the emergence of problems such as pesticide residues and environmental pollution.

[0004] Compounding different fungicidal active ingredients is an effective and convenient way to prevent and treat resistant diseases. Screening for synergistic compound combinations can improve the control effect on the target, reduce the amount of pesticides used, and reduce the cost of prevention and treatment. In addition, it can also delay the development of drug resistance in pathogens, which is one of the important means of comprehensive prevention and treatment of harmful pathogens.

[0005] Copper gluconate is a bioavailable form of copper, which is highly soluble in water, has good systemic activity, excellent protection, treatment and eradication activities, and its antibacterial mechanism is mainly to prevent the initiation of protein translation, thereby affecting protein synthesis and causing bacterial death. Prior art CN114223664B discloses a composition containing fluopicolide and copper gluconate, and its preparation and use, which discloses that when fluopicolide and copper gluconate are compounded in a certain weight ratio, a synergistic bactericidal effect can occur, and can be used to prevent and control diseases such as cucumber bacterial angular leaf spot, wheat scab and mango powdery mildew.

[0006] So far, there are no reports on the combination of copper gluconate with aureomycin or thiophanate-methyl.

[0007] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention

[0008] The object of the present invention is to provide a pesticide composition for preventing and controlling strawberry bacterial wilt, which has a synergistic effect on strawberry bacterial wilt pathogens, helps to delay the development of drug resistance of strawberry bacterial wilt pathogens, and can effectively alleviate the problems caused by long-term irrational use of chemical agents.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A fungicide pesticide composition, wherein the active ingredients of the fungicide pesticide composition are compounded with copper gluconate and aureomycin or thiophanate-methyl.

[0011] Preferably, the mass ratio of copper gluconate to aureomycin is 1-19:9-1.

[0012] Preferably, the mass ratio of copper gluconate to thiophanate-methyl is 1-40:20-1.

[0013] The invention also provides use of the fungicidal pesticide composition in preventing and controlling crop diseases.

[0014] More specifically, the crop diseases include strawberry bacterial wilt, which is caused by infection of Pseudomonas solanacearum.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) The present invention compounds copper gluconate with existing fungicides aureomycin or thiophanate-methyl in a certain mass ratio, and measures the effect of the compound combination on strawberry bacterial wilt by indoor turbidity method. The results show that the co-toxicity coefficient of the compound combination on strawberry bacterial wilt pathogen is greater than 120, indicating that the pesticide composition of the present invention has a synergistic effect on strawberry bacterial wilt. Compared with a single component, the control effect on strawberry bacterial wilt can be improved, and the use amount of the agent and the control cost can be reduced.

[0017] (2) Compared with a single component, the pesticide composition of the present invention can reduce the risk of drug resistance, delay the generation and development of drug resistance of strawberry bacterial wilt pathogen, effectively alleviate the problems caused by the long-term irrational use of chemical agents, and provide support for the comprehensive prevention and control of strawberry bacterial wilt. DETAILED DESCRIPTION

[0018] Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0019] 1. Test culture medium

[0020] NA medium: 5 g peptone, 3 g beef extract, 5 g sucrose, 15 g agar powder, add distilled water to 1 L, heat to dissolve, adjust the pH to 7.0, package, and sterilize at 121°C high pressure wet heat for 23 min.

[0021] NB medium: 5 g peptone, 3 g beef extract, 5 g sucrose, add distilled water to 1 L, heat to dissolve, adjust the pH to 7.0, package, and sterilize at 121°C for 23 min.

[0022] 2. Test pathogens

[0023] Pseudomonas solanacarum, isolated from diseased strawberry samples.

[0024] 3. Test reagents

[0025] 98% copper gluconate technical, 94% aureomycin technical, 95% thiophanate-methyl technical, all of the above agents are commercially available.

[0026] 4. Test methods

[0027] The toxicity of copper gluconate, aureomycin, thiophanate-methyl and their combined combinations against the tested pathogens was determined by turbidity method.

[0028] Pick a single colony of the test pathogen and place it in 30 mL NB medium. Incubate the culture at 28°C, 200 rpm, and shake until the test pathogen reaches the logarithmic growth phase. Then adjust the concentration of the bacterial suspension to 1×10 7 cfu / mL. Add 100 μL of bacterial suspension to 200 mL of NB medium, shake and culture at 28°C, 200 rpm for 2 h, mix well, and dispense into 100 mL Erlenmeyer flasks, 30 mL per flask.

[0029] After the test agent is dissolved, it is diluted with 0.1% Tween-80 aqueous solution to form a single-dose mother solution. Multiple groups of ratios are set, and each single agent and each group of mixed agents are diluted with 0.1% Tween-80 aqueous solution to form 5 mass concentration gradients. 1mL of the prepared drug solution is added to the packed conical flask, and the treatment with 0.1% Tween-80 aqueous solution is set as the blank control, and each treatment is repeated 4 times. The initial turbidity of each treatment is measured, and then it is placed at 28°C and 200rpm to continue to be cultured. When the blank control treatment reaches the logarithmic growth phase, the final turbidity of each treatment is measured, and the antibacterial rate of each agent treatment on the test bacteria is calculated.

[0030]

[0031] 5. Data Analysis

[0032] The logarithmic value of the drug concentration is x, the probability value of the inhibition rate is y, and the toxicity regression equation of the inhibition probability value and the concentration of the test drug is calculated to obtain the EC of each treatment drug. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated using the Sun Yunpei method.

[0033] The synergistic effect of mixed drugs was evaluated according to the co-toxicity coefficient (CTC), that is, CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The experimental results are shown in Table 1-2.

[0034] Table 1 Indoor bioactivity determination of copper gluconate and aureomycin against Pseudomonas solanacearum

[0035] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Copper Gluconate 1.1874 100.0000 -- -- Aureomycin 6.5075 18.2466 -- -- Copper Gluconate 1:Aureomycin 9 3.5856 33.1158 26.4220 125.3343 Copper Gluconate 1:Aureomycin 6 2.7507 43.1672 29.9257 144.2479 Copper Gluconate 1:Aureomycin 3 2.3418 50.7046 38.6850 131.0705 Copper Gluconate 1:Aureomycin 1 1.6442 72.2175 59.1233 122.1472 Copper Gluconate 3:Aureomycin 1 0.9737 121.9472 79.5617 153.2738 Copper Gluconate 6:Aureomycin 1 0.7008 169.4349 88.3209 191.8400 Copper Gluconate 9:Aureomycin 1 0.9417 126.0911 91.8247 137.3173 Copper Gluconate 19:Aureomycin 1 1.0243 115.9231 95.9123 120.8636

[0036] It can be seen from Table 1 that when the mass ratio of copper gluconate to aureomycin is 1-19:9-1, the co-toxicity coefficient of the compound combination to the strawberry wilt pathogen Pseudomonas solanacearum is greater than 120, indicating that copper gluconate and aureomycin have a synergistic effect on the strawberry wilt pathogen Pseudomonas solanacearum when compounded in a mass ratio of 1-19:9-1.

[0037] Table 2 Indoor bioactivity determination of copper gluconate and thiophanate-methyl against Pseudomonas solanacearum

[0038] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Copper Gluconate 1.1874 100.0000 -- -- Thiamphenicol 9.6238 12.3382 -- -- Copper Gluconate 1: Thiamphenicol 20 5.6531 21.0044 16.5125 127.2028 Copper gluconate 1: Thiamphenicol 10 1.7261 68.7909 20.3074 338.7477 Copper Gluconate 1: Thiamphenicol 5 0.9626 123.3534 26.9485 457.7381 Copper Gluconate 1: Thiamphenicol 3 2.4844 47.7942 34.2536 139.5305 Copper Gluconate 1: Thiamphenicol 1 1.2240 97.0098 56.1691 172.7103 Copper Gluconate 3: Thiamphenicol 1 1.0813 109.8123 78.0845 140.6325 Copper Gluconate 5: Thiamphenicol 1 0.8455 140.4376 85.3897 164.4667 Copper Gluconate 10: Thiamphenicol 1 0.5341 222.3179 92.0307 241.5692 Copper Gluconate 20: Thiamphenicol 1 0.3492 340.0344 95.8256 354.8470 Copper Gluconate 30: Thiamphenicol 1 0.6786 174.9779 97.1722 180.0699 Copper Gluconate 40: Thiamphenicol 1 0.8993 132.0360 97.8619 134.9208

[0039] It can be seen from Table 2 that when the mass ratio of copper gluconate to thiophanate-methyl is 1-40:20-1, the co-toxicity coefficient of the compound combination to the strawberry wilt pathogen Pseudomonas solanacearum is greater than 120, indicating that copper gluconate and thiophanate-methyl have a synergistic effect on the strawberry wilt pathogen Pseudomonas solanacearum when compounded in a mass ratio of 1-40:20-1.

[0040] The invention compounds copper gluconate with existing fungicides aureomycin or thiophanate-methyl in a certain mass ratio, and measures the effect of the compound combination on strawberry bacterial wilt by an indoor turbidity method. The results show that the co-toxicity coefficient of the compound combination on strawberry bacterial wilt pathogen is greater than 120, indicating that the pesticide composition of the invention has a synergistic effect on strawberry bacterial wilt, and can improve the control effect on strawberry bacterial wilt compared with a single component, and reduce the use amount of the agent and the control cost.

Claims

1. A fungicidal pesticide composition, characterized in that: The active ingredients of the bactericidal pesticide composition are prepared by compounding copper gluconate with aureomycin or thiamethoxam.

2. The fungicidal pesticide composition according to claim 1, characterized in that: The mass ratio of copper gluconate to aureomycin is 1-19:9-1.

3. The fungicidal pesticide composition according to claim 1, characterized in that: The mass ratio of the copper gluconate to thiophanate-methyl is 1-40:20-1.

4. Use of the fungicidal pesticide composition according to any one of claims 1 to 3 in preventing and controlling crop diseases.

5. The use according to claim 4, characterized in that The crop diseases include strawberry bacterial wilt, which is caused by infection of Pseudomonas solanacearum.

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