Dalbergia odorifera edgeworthia chrysantha liquid and method for promoting rapid formation of dalbergia odorifera heartwood

Abstract

The invention provides dalbergia odorifera edgeworthia chrysantha liquid and a method for promoting rapid formation of dalbergia odorifera heartwood. The dalbergia odorifera edgeworthia chrysantha liquid is prepared from the following raw materials in parts by weight: 3-5 parts of rice, 0.8-1.2 parts of olive peel, 1-2 parts of herba ecliptae and 1-2 parts of red-knees herb. The method for promoting rapid formation of the dalbergia odorifera heartwood comprises the following steps that a dalbergia odorifera tree is selected, transverse drilling is conducted on the tree trunk in the horizontal direction of the diameter of the cross section of the tree trunk through a drill bit, and the depth of transverse drilling is 60%-75% of the diameter of the tree trunk; the dalbergia odorifera and edgeworthia chrysantha liquid begins to be injected into the hole in a dripping mode within 15 min after drilling, and the injection amount of the dalbergia odorifera and edgeworthia chrysantha liquid ranges from 250 ml to 300 ml. After being treated by the method, the heartwood formed by the dalbergia odorifera is large in diameter, the content of ethanol extract, the yield of volatile oil and the content of 2-(2-phenethyl) chromone in the volatile oil are obviously improved, and the quality of the heartwood is effectively improved.

Description

Technical Field

[0001] The invention relates to the field of Dalbergia odorifera, and in particular to a Dalbergia odorifera fragrance-forming liquid and a method for promoting the rapid formation of Dalbergia odorifera heartwood. Background Art

[0002] Dalbergia odorifera T.Chen, also known as rosewood, sandalwood, fragrant redwood, palm, fragrant branch, rosewood, yellow rosewood, is a national second-level protected plant. Dalbergia odorifera is a semi-deciduous tree, a species of the genus Dalbergia in the Fabaceae family, with a tree height of 15 to 20 meters and a breast diameter of 0.8 meters; the bark is brown or light brown, rough, with longitudinal cracks, and becomes yellow-brown and smooth after peeling; the twigs are smooth and the old branches are rough. The existing method is slow to form the heartwood of Dalbergia odorifera, and it is of great significance to develop a new Dalbergia odorifera fragrance liquid and a method to promote the rapid formation of Dalbergia odorifera heartwood. Summary of the invention

[0003] In view of this, the present invention provides a Dalbergia odorifera fragrance-forming liquid and a method for promoting the rapid formation of Dalbergia odorifera heartwood to solve the above problems.

[0004] The technical solution of the present invention is achieved in this way:

[0005] The invention discloses a Dalbergia odorifera fragrance-forming liquid, which is prepared from the following raw materials in parts by weight: 3-5 parts of rice, 0.8-1.2 parts of Elemi bark, 1-2 parts of Eclipta prostrata, and 1-2 parts of Polygonum hydropiper.

[0006] Elemi is the bark of olive (Canarium album (Lour.) Rauesch.), a plant of the genus Olive, and contains elemenine, β-elemene, etc.

[0007] Eclipta prostrasta L. is the above-ground part of the Asteraceae plant, which contains nicotine, saponins, tannins, isoflavone glycosides, and wedelilactone.

[0008] Polygonum flaccidum Meissn. is the whole herb of Polygonum flaccidum Meissn., a plant of the Polygonaceae family. It contains hyperoside, rutin, quercetin, isorhamnetin, kaempferol, etc.

[0009] A further solution is that the Dalbergia odorifera fragrance liquid is prepared by fermenting rice, Elemi bark, Eclipta prostrata and Polygonum hydropiper.

[0010] A further solution is that the preparation method of Dalbergia odorifera fragrance liquid comprises the following steps: respectively weighing rice, Elemi bark, Eclipta prostrata, and Polygonum hydropiper by weight, mixing and crushing, adding water at a solid-liquid ratio of 1:1-3 (g / ml), placing in a sealed fermentation tank, sealing and fermenting for 35-45 days, filtering to obtain fermentation liquid, adding the composite strain, uniformly mixing, and sealing and fermenting for 7-10 days, filtering, and taking the filtrate to obtain the Dalbergia odorifera fragrance liquid.

[0011] In a further embodiment, the composite bacterial species is composed of Bacillus natto and Musicillium theobromae.

[0012] In a further embodiment, the ratio of the fermentation liquid to Bacillus natto and Musicillium theobromae is 1000 ml: 1-3 g: 1-3 g.

[0013] A further solution is that the fermentation temperature is 30-40°C.

[0014] A method for promoting the rapid formation of Dalbergia odorifera heartwood comprises the following steps: selecting a Dalbergia odorifera tree, and using a drill to drill a horizontal hole in the trunk along the horizontal direction of the cross-sectional diameter of the tree trunk; and within 15 minutes after drilling, drip-injecting the Dalbergia odorifera fragrance-forming liquid described in any one of the present invention into the hole in the form of an infusion.

[0015] A further solution is that the depth of the transverse borehole is 60%-75% of the trunk diameter.

[0016] A further solution is that the injection amount of the Dalbergia odorifera fragrance liquid is 250-300 ml.

[0017] A further solution is that the drip rate is 15 to 20 drops per minute.

[0018] A further solution is that the drilling position is 10-20 cm above the ground.

[0019] A further solution is that the drill bit diameter is 3.5-4.5 mm.

[0020] A further solution is to specifically select Dalbergia odorifera trees: Dalbergia odorifera trees with a trunk diameter of more than 15 cm.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] (1) The present invention uses rice, elemi, black mulberry, and hot pepper to ferment and prepare Dalbergia odorifera fragrance liquid, which can effectively accelerate the formation of Dalbergia odorifera heartwood, significantly increase the rate of heartwood formation, accumulate lipid substances in the wood, increase the content of 2-(2-phenylethyl) chromone in the heartwood, and greatly improve the quality of the heartwood. Moreover, the longer the age, the more heartwood is formed, and the better the quality.

[0023] (2) The rice, elemi, radix polygoni odorifera and polygonum hydropiper are fermented with a composite strain of Bacillus natto and Musicillium theobromae to prepare a Dalbergia odorifera fragrance liquid rich in active ingredients, which can better promote the accumulation of lipid substances in the xylem and accelerate the formation of Dalbergia odorifera heartwood.

[0024] (3) After being treated by the drilling method of the present invention, a certain amount of Dalbergia odorifera fragrance liquid is input, and the heartwood formed by Dalbergia odorifera has a large diameter, and the ethanol extract content of the heartwood, the yield of volatile oil, and the content of 2-(2-phenylethyl) chromone in the volatile oil are all significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the punching method of Dalbergia odorifera of the present invention.

[0026] In the figure, 1 is the trunk of Dalbergia odorifera, and 2 is the hole formed after drilling.

[0027] Figure 2 These are photos of the heartwood formation of Dalbergia odorifera with the same growth years. In the figure, the left side shows the naturally formed heartwood, and the right side shows the heartwood formed by the treatment of Example 4 of the present invention. DETAILED DESCRIPTION

[0028] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0029] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0030] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0031] Bacillus natto was purchased from China Center for Type Culture Collection with the collection number CCTCCCB 20081702.

[0032] Musicillium theobromae was purchased from Guangdong Provincial Culture Collection Center, with the collection number GDMCC NO:3.519.

[0033] Example 1

[0034] The formula and preparation method of Dalbergia odorifera fragrance liquid are as follows: by weight, 4 parts of rice, 1 part of Elemi peel, 1.5 parts of Polygonum hydropiper, and 1.5 parts of Polygonum hydropiper are taken, crushed together, water is added at a solid-liquid ratio of 1:2 (g / ml), and the mixture is placed in a sealed fermentation tank, and sealed and fermented at 35°C±2°C for about 40 days; the mixture is taken out and filtered, and the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is added with Bacillus natto (2g) and Verticillium Cocos (2g), and the mixture is evenly mixed, and the mixture is sealed and fermented at 35°C±2°C for 7 days, and the filtrate is taken to obtain Dalbergia odorifera fragrance liquid.

[0035] Example 2

[0036] The formula and preparation method of Dalbergia odorifera fragrance liquid are as follows: by weight, 3 parts of rice, 1 part of Elemi peel, 2 parts of Ecliptae, and 1 part of Polygonum hydropiper are taken, crushed together, water is added at a solid-liquid ratio of 1:2 (g / ml), placed in a sealed fermentation tank, and sealed and fermented at 35°C±2°C for about 40 days; taken out and filtered, the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is added with Bacillus natto (1g) and Verticillium Cocos (3g), evenly mixed, and sealed and fermented at 35°C±2°C for 7 days, and then the filtrate is taken to obtain Dalbergia odorifera fragrance liquid.

[0037] Example 3

[0038] The formula and preparation method of Dalbergia odorifera fragrance liquid are as follows: according to weight, 5 parts of rice, 1 part of Elemi peel, 1 part of Ecliptae, and 2 parts of Polygonum hydropiper are taken, crushed together, water is added according to a solid-liquid ratio of 1:2 (g / ml), placed in a sealed fermentation tank, and sealed and fermented at 35°C±2°C for about 40 days; taken out and filtered, and the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is added with Bacillus natto (3g) and Verticillium Cocos (1g), evenly mixed, and sealed and fermented at 35°C±2°C for 7 days, and then the filtrate is taken to obtain Dalbergia odorifera fragrance liquid.

[0039] Example 4

[0040] A method for promoting the rapid formation of heartwood of Dalbergia odorifera, comprising the following steps: selecting a Dalbergia odorifera tree with a trunk diameter ranging from 15 to 16 cm, and using a 4 mm drill bit to make horizontal holes in the trunk along the horizontal direction of its cross-sectional diameter at a height of 10 to 15 cm from the ground, with the hole depth being two-thirds of the trunk; within 15 minutes after drilling, begin dripping the Dalbergia odorifera fragrance-forming liquid of Example 1 into the hole in the form of an infusion, with a total amount of 280 ml of liquid injected and a drip rate of 15 to 20 drops per minute. Seven days after the solution is completely injected into the tree, regular fertilization and watering are carried out in a timely manner according to weather conditions to ensure that the plant has sufficient nutrients and water. The heartwood can be collected one year after the tree has been treated. The drilling method is as follows: Figure 1 shown.

[0041] like Figure 2 It can be seen that compared with the naturally formed heartwood, the rate of heartwood formation after one year of treatment using Example 4 of the present invention is significantly faster and has a better effect. The Dalbergia odorifera fragrance-forming liquid prepared by the present invention can effectively accelerate the formation of Dalbergia odorifera heartwood, accumulate lipid substances in the wood, and greatly improve the quality of the heartwood.

[0042] Example 5

[0043] A method for promoting the rapid formation of Dalbergia odorifera heartwood comprises the following steps: selecting a Dalbergia odorifera tree with a trunk diameter ranging from 15 to 16 cm, drilling a horizontal hole in the trunk along the horizontal direction of its cross-sectional diameter at a height of 10 to 15 cm from the ground using a 4 mm drill bit, with the hole depth being two-thirds of the trunk; within 15 minutes after drilling the hole, dripping the Dalbergia odorifera fragrance-forming liquid of Example 2 into the hole in the form of an infusion, with a total amount of 280 ml of liquid injected and a drip rate of 15 to 20 drops per minute; and sealing the hole with environmentally friendly glue after the infusion. Seven days after the solution is completely injected into the tree, regular fertilization and watering are carried out in time according to weather conditions to ensure that the plant has sufficient nutrients and water, and the heartwood can be collected one year after the tree is treated.

[0044] Example 6

[0045] A method for promoting the rapid formation of Dalbergia odorifera heartwood comprises the following steps: selecting a Dalbergia odorifera tree with a trunk diameter ranging from 15 to 16 cm, drilling a horizontal hole in the trunk along the horizontal direction of its cross-sectional diameter at a height of 10 to 15 cm from the ground using a 4 mm drill bit, with the hole depth being two-thirds of the trunk; within 15 minutes after drilling the hole, dripping the Dalbergia odorifera fragrance-forming liquid of Example 3 into the hole in the form of an infusion, with a total amount of 280 ml of liquid injected and a drip rate of 15 to 20 drops per minute. Seven days after the solution is completely injected into the tree, regular fertilization and watering are carried out in a timely manner according to weather conditions to ensure that the plant has sufficient nutrients and water, and the heartwood can be collected one year after the tree is treated.

[0046] Comparative Example 1

[0047] The main difference from Example 1 is that the formula of Dalbergia odorifera incense liquid is different, does not contain Elemi bark, and is prepared from the following raw materials by weight: 4 parts of rice, 2 parts of Polygonum hydropiper, and 2 parts of Polygonum hydropiper. Other treatments are the same as in Example 4.

[0048] Comparative Example 2

[0049] The main difference from Example 1 is that the formula of Dalbergia odorifera incense liquid is different, does not contain black mulberry, and is prepared from the following raw materials by weight: 4 parts of rice, 2 parts of Elemi peel, and 2 parts of Polygonum hydropiper. Other treatments are the same as in Example 4.

[0050] Comparative Example 3

[0051] The main difference from Example 1 is that the formula of Dalbergia odorifera incense liquid is different, does not contain Polygonum hydropiper, and is prepared from the following raw materials by weight: 4 parts of rice, 2 parts of Elemi peel, and 2 parts of Rhizoma Cibotii. Other treatments are the same as in Example 4.

[0052] Comparative Example 4

[0053] The main difference from Example 1 is that the proportion of the components of the Dalbergia odorifera fragrance liquid formula is different from that of Example 1, and the Dalbergia odorifera fragrance liquid is prepared by the following raw materials: 4 parts of rice, 1.5 parts of Elemi bark, 0.5 parts of Eclipta prostrata, and 2.5 parts of Polygonum hydropiper are weighed by weight. Other treatments are the same as those in Example 4.

[0054] Comparative Example 5

[0055] The main difference from Example 1 is that only Bacillus natto is added in the preparation process of Dalbergia odorifera fragrance-forming liquid, and Verticillium theobroma is not added.

[0056] The formula and preparation method of Dalbergia odorifera fragrance liquid are as follows: 4 parts of rice, 1 part of Elemi peel, 1.5 parts of Polygonum hydropiper, and 1.5 parts of Polygonum hydropiper are ground together, water is added according to a solid-liquid ratio of 1:2 (g / ml), and the mixture is placed in a sealed fermentation tank, and sealed and fermented at 35°C±2°C for about 40 days; the mixture is taken out and filtered, and the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is added with Bacillus natto (4 g), and the mixture is evenly mixed, and the mixture is sealed and fermented at 35°C±2°C for 7 days, and the filtrate is taken to obtain Dalbergia odorifera fragrance liquid.

[0057] The other treatments were the same as those in Example 4.

[0058] Comparative Example 6

[0059] The main difference from Example 1 is that only Verticillium Cocoa was added in the preparation process of Dalbergia odorifera fragrance-forming liquid, and Bacillus natto was not added.

[0060] The formula and preparation method of Dalbergia odorifera fragrance liquid are as follows: 4 parts of rice, 1 part of Elemi peel, 1.5 parts of Polygonum hydropiper, and 1.5 parts of Polygonum hydropiper are ground together, water is added according to a solid-liquid ratio of 1:2 (g / ml), and the mixture is placed in a sealed fermentation tank, and sealed and fermented at 35°C±2°C for about 40 days; the mixture is taken out and filtered, and the filtrate is placed in a sterile brown glass bottle or other light-proof container for storage to obtain a fermentation liquid; 1000 ml of the fermentation liquid is added with Verticillium cocoa (4 g), the mixture is evenly mixed, and the mixture is sealed and fermented at 35°C±2°C for 7 days, and the filtrate is taken to obtain the Dalbergia odorifera fragrance liquid.

[0061] The other treatments were the same as those in Example 4.

[0062] Comparison example

[0063] The main difference from Example 4 is that the Dalbergia odorifera fragrance liquid was not injected, but tap water of equal volume was used instead. Other treatments were consistent with Example 4.

[0064] The test was carried out according to the methods of Examples 4-6 and Comparative Examples 1-6, respectively. The trunk diameter of Dalbergia odorifera was in the range of 15-16 cm before the test. The heartwood was collected one year after the tree was treated, and the quality of the heartwood was tested. The test method was: the hot soaking method and the volatile oil determination method in the alcohol-soluble extract determination method specified in the Pharmacopoeia of the People's Republic of China (2020 edition) were used to detect the ethanol extract content and volatile oil yield of the heartwood, and the HPLC method was used to detect the 2-(2-phenylethyl) chromone content in the volatile oil. The results are shown in Table 1.

[0065] Table 1 Comparison of the quality of Dalbergia odorifera heartwood

[0066] Group Ethanol extract content Volatile oil yield 2-(2-phenylethyl)chromone content in volatile oil Example 4 24.93％ 2.70％ 92.91％ Example 5 22.86％ 2.52％ 90.74％ Example 6 23.16％ 2.56％ 91.72％ Comparative Example 1 18.05％ 1.94％ 82.48％ Comparative Example 2 17.76％ 2.07％ 85.03％ Comparative Example 3 17.98％ 2.02％ 84.91％ Comparative Example 4 18.21％ 2.16％ 86.16％ Comparative Example 5 14.46％ 1.90％ 87.56％ Comparative Example 6 17.43％ 1.84％ 87.07％ Comparison example 12.48％ 1.62％ 75.11％

[0067] As can be seen from Table 1, compared with the control example, the ethanol extract content, volatile oil yield and 2-(2-phenylethyl)chromone content in the volatile oil of the heartwood formed by Examples 4-6 are significantly improved, and are better than those of Comparative Examples 1-6.

[0068] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A Dalbergia odorifera fragrance liquid, characterized in that: The invention comprises the following raw materials in parts by weight: 3-5 parts of rice, 0.8-1.2 parts of elemi peels, 1-2 parts of eclipta prostrata and 1-2 parts of polygonum hydropiper.

2. The Dalbergia odorifera fragrance-forming liquid according to claim 1, characterized in that The Dalbergia odorifera fragrance liquid is prepared by fermenting rice, Elemi bark, Eclipta prostrata and Polygonum hydropiper.

3. The Dalbergia odorifera fragrance-forming liquid according to claim 1, characterized in that: The preparation method of the Dalbergia odorifera fragrance-forming liquid comprises the following steps: respectively weighing rice, Elemi bark, Eclipta prostrata, and Polygonum hydropiper by weight, mixing and crushing, adding water at a solid-liquid ratio of 1:1-3 (g / ml), placing in a sealed fermentation tank, sealing and fermenting for 35-45 days, filtering to obtain fermentation liquid, adding composite strains, uniformly mixing, sealing and fermenting for 7-10 days, filtering, and taking the filtrate to obtain the Dalbergia odorifera fragrance-forming liquid.

4. The Dalbergia odorifera fragrance-forming liquid according to claim 1, characterized in that: The composite bacteria are composed of Bacillus natto and Musicillium theobromae; the ratio of the fermentation liquid to Bacillus natto and Musicillium theobromae is 1000ml:1-3g:1-3g.

5. The Dalbergia odorifera fragrance-forming liquid according to claim 1, characterized in that: The fermentation temperature is 30-40°C.

6. A method for promoting the rapid formation of Dalbergia odorifera heartwood, characterized in that: The following steps are involved: A Dalbergia odorifera tree is selected, and a transverse hole is drilled in the trunk horizontally along the cross-sectional diameter thereof using a drill bit, wherein the depth of the transverse hole is 60%-75% of the trunk diameter; within 15 minutes after the drilling, the Dalbergia odorifera fragrance liquid according to any one of claims 1 to 5 is drip-injected into the hole in the form of an infusion, wherein the injection amount of the Dalbergia odorifera fragrance liquid is 250-300 ml.

7. The method for promoting the rapid formation of Dalbergia odorifera heartwood according to claim 6, characterized in that: The drip rate is 15 to 20 drops per minute.

8. The method for promoting the rapid formation of Dalbergia odorifera heartwood according to claim 6, characterized in that: The drilling position is 10-20cm above the ground.

9. The method for promoting the rapid formation of Dalbergia odorifera heartwood according to claim 6, characterized in that: The drill bit diameter is 3.5-4.5mm.

10. The method for promoting the rapid formation of Dalbergia odorifera heartwood according to any one of claims 6 to 9, characterized in that: The Dalbergia odorifera tree is a Dalbergia odorifera tree with a trunk diameter of more than 15 cm.

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