Anti-depression gel candy and preparation method thereof

Abstract

The invention discloses an anti-depression gel candy and a preparation method thereof, and the anti-depression gel candy comprises the following components in parts by weight: 10-40 parts of edible gum, 40-60 parts of a natural sweetening agent, 0.2-0.8 part of citric acid and 5-30 parts of a day lily extract, wherein the hemerocallis citrina extract is obtained by performing alcohol extraction on hemerocallis citrina. The hemerocallis citrina baroni gel candy which is lasting in anti-depression effect and small in side effect is prepared aiming at the problems that an existing anti-depression drug is short in drug effect lasting time, easy to repeat and particularly serious in side effect, and the total flavone content is increased by optimizing a hemerocallis citrina baroni extraction process; the effective dose and the anti-depression effect are determined through in-vitro anti-depression verification of the day lily extract, then the taste of the day lily gel candy is adjusted according to a certain proportion, only natural sugar alcohol is added into the day lily gel candy, the acceptance degree of people is expanded, and the day lily gel candy has a certain nutritional value.

Description

Technical Field

[0001] The present application belongs to the technical field of food preparation, and in particular relates to an antidepressant gel candy and a preparation method thereof. Background Art

[0002] As the pace of society accelerates, people are under increasing mental pressure, and the incidence of depression is increasing. At present, most of the drugs used in clinical treatment of depression are drugs that regulate monoamine neurotransmitters. Although they can have good therapeutic effects, they are difficult to get rid of the dilemma of short duration of drug effects, easy recurrence and serious side effects. Traditional Chinese medicine has the advantages of delaying the course of the disease, improving the efficacy of Western medicine, and reducing side effects in the prevention and treatment of depression. Traditional Chinese medicine believes that depression belongs to the category of "depression syndrome". With the in-depth research of traditional Chinese medicine in the prevention, treatment, prevention and treatment of depression, daylily has been proven to have a good effect in improving depression.

[0003] As a special vegetable that is both edible and medicinal, daylily is rich in nutrition and contains carbohydrates, proteins, vitamins, inorganic salts and a variety of essential amino acids for the human body. Many studies have shown that daylily and its extracts have good effects in treating depression, among which flavonoids have the effects of improving sleep and anti-depression. The present invention has prepared the daylily, which is rich in nutrition and can improve depression, into a daylily extract gel candy with excellent taste, good tolerance and less side effects when eaten in moderation. Summary of the invention

[0004] The technical problem to be solved by the present application is to overcome the defects of the existing antidepressant drugs in the prior art, such as the short duration of drug effect, easy recurrence, and especially serious side effects, and to provide an antidepressant gel candy and a preparation method thereof; the gel candy prepared by the present application is an elastic and chewy product made of edible gum (or starch), white sugar and starch syrup (or other edible sugar) as the main ingredients. The product contains sugar, and the applicable population is limited. In order to expand the suitable edible population, the daylily extract gel candy prepared by the present invention preferably has natural sugar alcohols in its formula, and no additional sucrose is added, so it can also be eaten in moderation by people with three highs.

[0005] This application adopts the following technical solutions to solve the above technical problems:

[0006] The present application provides an antidepressant gel candy, which comprises the following components by weight: 10-40 parts of edible gum, 40-60 parts of natural sweetener, 0.2-0.8 parts of citric acid, and 9-18 parts of daylily extract;

[0007] Wherein, the daylily extract is obtained by alcohol extraction of daylily.

[0008] In some embodiments, the edible gum includes at least one of gelatin, pectin, carrageenan, and xanthan gum;

[0009] Wherein, when the edible gum includes the gelatin and the pectin, the mass ratio of the gelatin to the pectin is 4:1.

[0010] In some embodiments,

[0011] Preferably, the sugar alcohol includes at least one of sorbitol, xylitol, lactitol, mannitol and maltitol;

[0012] Wherein, when the sugar alcohol includes the sorbitol and the xylitol, the mass ratio of the sorbitol to the xylitol is 3:2.

[0013] In some embodiments, the method for preparing the daylily extract comprises: crushing the daylily and adding ethanol for extraction;

[0014] Wherein, the particle size of the daylily powder is 200 mesh;

[0015] Wherein, the mass fraction of the ethanol is 70%;

[0016] The extraction time is 0.5 to 2 hours, and the extraction times are 2 to 4 times.

[0017] The present application also provides a method for preparing an antidepressant gel candy, comprising the following preparation steps:

[0018] (1) adding the edible gum into water, soaking and constant temperature water bathing to obtain a swollen edible gum, and setting aside;

[0019] (2) adding the natural sweetener to the water, dissolving it and then heating it to obtain a sugar solution, which is then cooled and set aside;

[0020] (3) dissolving the citric acid and the daylily extract in water to obtain a mixture A for later use;

[0021] (4) After the edible gum swollen product, the sugar solution and the mixture A are stirred, demoulding is performed.

[0022] In some embodiments, the method for preparing the antidepressant gel candy satisfies at least one of the following conditions:

[0023] In step (1), the mass ratio of the edible gum to the water is 1:(3-6);

[0024] In step (1), the soaking is done at room temperature;

[0025] In step (1), the soaking time is 0.5 to 1.5 hours;

[0026] In step (1), the temperature of the constant temperature water bath is 70° C.

[0027] In step (1), the constant temperature water bath is kept for 30 minutes.

[0028] In some embodiments, the method for preparing the antidepressant gel candy satisfies at least one of the following conditions:

[0029] In step (2), the mass ratio of the natural sweetener to the water is 1:(1-3);

[0030] In step (2), the heating temperature is 90 to 105° C.

[0031] In step (2), when the sugar solution shows a stringy state during the heating process, the heating is stopped;

[0032] In step (2), the cooling to 75°C is sufficient.

[0033] In some embodiments, in step (3), the mass ratio of the citric acid and the daylily extract to the water is 1:(1-3).

[0034] In some embodiments, the method for preparing the antidepressant gel candy satisfies at least one of the following conditions:

[0035] In step (4), the stirring time is 0.1-0.5h;

[0036] In step (4), the stirring speed is 30-60 rpm.

[0037] In some embodiments, step (4) also includes cooling and demolding operations.

[0038] On the basis of conforming to the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present application.

[0039] The reagents and raw materials used in this application are commercially available.

[0040] The positive progress of the present application is that: in view of the short duration of the efficacy of existing antidepressant drugs, easy recurrence, and especially serious side effects, the present application prepares a daylily gel candy with long-lasting antidepressant effect and small side effects, and increases the total flavonoid content by optimizing the daylily extraction process; the effective dose and antidepressant effect of the daylily extract are verified in vitro for antidepressant effect, and the taste of the daylily gel candy is adjusted according to a certain ratio. Only natural sugar alcohols are added to the daylily gel candy, which expands the acceptance of the population and has certain nutritional value. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The present application can be better understood by referring to the description given below in conjunction with the accompanying drawings. The accompanying drawings, together with the following detailed description, are included in this specification and form a part of this specification, and are used to further illustrate the preferred embodiments of the present application and explain the principles and advantages of the present application.

[0042] in:

[0043] Figure 1 For different manufacturers;

[0044] Figure 2 Cell viability of daylily extracts from different manufacturers;

[0045] Figure 3 This is a comparison chart of ELISA test results. DETAILED DESCRIPTION

[0046] The present application is further described below by way of examples, but the present application is not limited to the scope of the examples. The experimental methods without specific conditions in the following examples are selected according to conventional methods and conditions, or according to the product instructions.

[0047] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0048] The raw materials in the following examples are all commercially available.

[0049] Example 1

[0050] A method for preparing an antidepressant gel candy, comprising the following components by weight:

[0051] 9 parts of daylily extract, 16 parts of gelatin, 4 parts of pectin, 24 parts of sorbitol, 16 parts of xylitol, and 0.4 parts of citric acid.

[0052] The preparation method of the daylily extract comprises the following steps:

[0053] The ratio of daylily powder to liquid was 1:10, 70% ethanol, ultrasonic extraction, each extraction was 1 hour, and extraction was performed twice.

[0054] The preparation method of the above candy is as follows:

[0055] (1) Take gelatin: pectin = 8:2, wherein gelatin is added with 3 times the amount of purified water and pectin is added with 10 times the amount of purified water, soak at room temperature for 1 hour, then bathe in a 70°C constant temperature water bath for 30 minutes to fully swell, and obtain an edible gum swellable product for later use;

[0056] (2) Add an appropriate amount of purified water according to the ratio of sorbitol to xylitol = 6:4 to fully dissolve the mixture and heat the mixture. The temperature is controlled within the range of 100-105°C. When the sugar solution appears to be in a stringy state, stop heating and cool the mixture to 75°C for later use.

[0057] (3) Weigh 0.4% citric acid and 9% daylily extract, dissolve them in twice the mass of pure water, and dissolve to obtain a mixture A for later use;

[0058] (4) Mixing the edible gum swelling material, the sugar solution, and the mixture A, stirring them thoroughly and evenly to remove all bubbles, pouring them into a mold, letting them stand and cool, demolding, and bagging them to obtain the daylily extract gel candy.

[0059] Example 2

[0060] A method for preparing an antidepressant gel candy, comprising the following components by weight:

[0061] 9 parts of daylily extract, 12 parts of gelatin, 8 parts of pectin, 20 parts of sorbitol, 20 parts of xylitol, and 0.4 parts of citric acid.

[0062] The preparation method of the daylily extract comprises the following steps:

[0063] The ratio of daylily powder to liquid was 1:10, 70% ethanol, ultrasonic extraction, each extraction was 1 hour, and extraction was performed twice.

[0064] The preparation method of the above candy is as follows:

[0065] (1) Take gelatin: pectin = 6:4, wherein gelatin is added with 3 times the amount of purified water and pectin is added with 10 times the amount of purified water, soak at room temperature for 1 hour, then bathe in a 70°C constant temperature water bath for 30 minutes to fully swell, and obtain an edible gum swellable product, which is set aside;

[0066] (2) Add an appropriate amount of purified water according to the ratio of sorbitol to xylitol = 5:5 to fully dissolve the mixture and heat the mixture. The temperature is controlled within the range of 100-105°C. When the sugar solution appears to be in a stringy state, stop heating, cool the mixture to 75°C at room temperature, and set aside.

[0067] (3) Weigh 0.4% citric acid and 9% daylily extract, dissolve them in twice the mass of pure water, and dissolve to obtain a mixture A for later use;

[0068] (4) Mixing the edible gum swelling material, the sugar solution, and the mixture A, stirring them thoroughly and evenly to remove all bubbles, pouring them into a mold, letting them stand and cool, demolding, and bagging them to obtain the daylily extract gel candy.

[0069] Example 3

[0070] A method for preparing an antidepressant gel candy, comprising the following components by weight:

[0071] 9 parts of daylily extract, 4 parts of gelatin, 16 parts of pectin, 16 parts of sorbitol, 24 parts of xylitol, and 0.4 parts of citric acid. The preparation method of the daylily extract comprises the following steps:

[0072] The ratio of daylily powder to liquid was 1:10, 70% ethanol, ultrasonic extraction, each extraction was 1 hour, and extraction was performed twice.

[0073] The preparation method of the above candy is as follows:

[0074] (1) Take gelatin: pectin = 2:8, wherein gelatin is added with 3 times the amount of purified water and pectin is added with 10 times the amount of purified water, soak at room temperature for 1 hour, then bathe in a 70°C constant temperature water bath for 30 minutes to fully swell, and obtain an edible gum swellable product, which is set aside;

[0075] (2) Add an appropriate amount of purified water according to the ratio of sorbitol to xylitol = 4:6 to fully dissolve the mixture and heat the mixture. The temperature is controlled within the range of 100-105°C. When the sugar solution appears to be in a stringy state, stop heating and cool the mixture to 75°C for later use.

[0076] (3) Weigh 0.4% citric acid and 9% daylily extract, dissolve them in twice the mass of pure water, and dissolve to obtain a mixture A for later use;

[0077] (4) Mixing the edible gum swelling material, the sugar solution, and the mixture A, stirring them thoroughly and evenly to remove all bubbles, pouring them into a mold, letting them stand and cool, demolding, and bagging them to obtain the daylily extract gel candy.

[0078] Effect Example 1

[0079] The total flavonoid content of Example 1 and each manufacturer was tested by ultraviolet spectrophotometry.

[0080] 1. Establishment of total flavonoid standard curve

[0081] Use rutin as the standard to make a standard curve. Accurately weigh 20.0 mg of rutin standard and dilute it to a 100 mL volumetric flask with 60% ethanol to obtain a 0.2 mg / mL rutin standard reference solution; accurately pipette 4.0, 4.5, 5.0, 5.5, 6.0, and 6.5 mL of rutin standard solution into 6 25 mL volumetric flasks, and add 12 mL of 60% ethanol, 0.7 mL of 5% NaNO, and 12 mL of 60% ethanol, 0.7 mL of 5% NaNO, and 1 mL of 5% NaNO. 2 The solution was shaken and allowed to stand for 6 min before adding 0.7 mL of 10% Al(NO 3 ) 3Solution, shake well, let stand for 6 minutes, add 5mL 10% NaOH solution, dilute to 25mL with 60% ethanol, shake well, let stand for 15 minutes, use the solution without rutin reference as blank reference, measure the absorbance at 245nm. Draw a standard curve with solution concentration (C) as the horizontal axis and absorbance (A) as the vertical axis.

[0082] 2. Determination of total flavonoid content in samples

[0083] Take 1 mL of the supernatant and place it in a 25 mL volumetric flask. Add 12 mL of 60% ethanol and 0.7 mL of 5% NaNO 2 The solution was shaken and allowed to stand for 6 min before adding 0.7 mL of 10% Al(NO 3 ) 3 Solution, shake well, let stand for 6 minutes, add 5mL 10% NaOH solution, dilute to 25mL with 60% ethanol, shake well, let stand for 15 minutes, use the solution without rutin reference as blank reference, and measure the absorbance at 245nm. Calculate the total flavonoids in the daylily extract according to the standard curve equation and the distillation volume.

[0084] The results of total flavonoid content test are as follows

[0085] in, Figure 1 Sample 1 is from Shanxi Shuohang Biotechnology Co., Ltd.; Sample 2 is from Shaanxi Shengheng Biological Plant Extract; Sample 3 is from Shaanxi Snow Biological Co., Ltd.; Sample 4 is from Shanxi Nanba Biotechnology Co., Ltd.

[0086] Table 1

[0087]

[0088]

[0089] Depend on Figure 1 It can be seen that the total flavonoids content of the daylily extract in the self-extraction (Example 1) is significantly higher than the total flavonoids content in samples 1-3.

[0090] Effect Example 2

[0091] The cell viability of Example 1 and each manufacturer was tested by CCK-8 method:

[0092] PC12 cells in the logarithmic growth phase were seeded in a 96-well plate at a density of 5000 cells per well, 100 μL of cell suspension was added, and the plate was placed in a CO 2Culture in a culture box; after the cells adhere to the wall, discard the old culture medium, add different concentrations of daylily extract for pretreatment for 1 hour, and then add appropriate concentrations of corticosterone to continue culturing for 24 hours; add an equal amount of CCK-8 reagent to each well, put it into a cell culture box and continue culturing for 1-4 hours, use an enzyme marker at a wavelength of 450nm to measure the OD value, and calculate the cell survival rate.

[0093] Cell survival rate (%) = (OD experimental group - OD zero adjustment group) / (OD control group - OD zero adjustment group) × 100%

[0094] Figure 2 Among them, sample 1 is from Shanxi Shuohang Biotechnology Co., Ltd.; sample 2 is from Shaanxi Shengheng Biological Plant Extract; sample 3 is from Shaanxi Snow Biological Co., Ltd.; sample 4 is from Shanxi Nanba Biotechnology Co., Ltd.

[0095] Model group: cultured with high concentration of corticosterone; Blank group: cells were cultured normally without any treatment factors; Fluoxetine group: fluoxetine was added after cells attached to the wall, and then corticosterone of appropriate concentration was added after pretreatment for 1 hour and cultured for 24 hours;

[0096] The self-extraction group (Example 1) was as follows: After the daylily extract cells in Example 1 adhered to the wall, high, medium and low doses of daylily extract were first added, and after pretreatment for 1 hour, appropriate concentrations of corticosterone were added and cultured for 24 hours.

[0097] Table 2

[0098]

[0099]

[0100] From Table 2 and Figure 2 It can be seen that the cell activity of the daylily extract in 0.50 mg / mL self-extraction (Example 1) is closest to that of the normal group, and the cell activity of the self-extraction group (Example 1) is higher than that of sample groups 1 to 4.

[0101] Effect Example 3

[0102] The in vitro antidepressant efficacy of Example 1 was verified by administering the daylily extract at different concentrations to perform antidepressant and anti-inflammatory activity experiments to determine its effective antidepressant dose (results are shown in Tables 1 and Figure 3 ,) The specific operations are:

[0103] (1) Cell culture

[0104] PC12 cells were cultured in high-glucose DMEM medium containing 10% fetal bovine serum (FBS), penicillin and streptomycin in an incubator at 37°C and 5% CO. 2 .

[0105] (2) Establishment of CORT injury model

[0106] PC12 cells in the logarithmic growth phase were inoculated into 96-well plates and placed in a CO 2 Cultured in an incubator; after the cells adhered to the wall, different concentrations of corticosterone (50, 100, 200, 300, 400, 500 μmol / L) were added to each well. After culturing for 24 h, the CCK-8 method was used to determine the cell survival rate and to determine the optimal concentration of corticosterone that damaged PC12 cells.

[0107] (3) Effects of different concentrations of daylily extract on the survival rate of cells damaged by corticosterone

[0108] PC12 cells in the logarithmic growth phase were inoculated into 96-well plates according to the following groups: ① Zero adjustment group: add equal amount of culture medium. ② Blank control group: cells were cultured normally without any treatment factors. ③ Corticosterone model group: after cells adhered to the wall, appropriate concentration of corticosterone was added and cultured for 24h. ④ Positive drug fluoxetine group; ⑤-⑦ Different doses of daylily extract + corticosterone group: after cells adhered to the wall, different doses of daylily gel candy were added first, pretreated for 1h, and then appropriate concentrations of corticosterone were added and cultured for 24h.

[0109] PC12 cells in the logarithmic growth phase were seeded in a 96-well plate at a density of 5000 cells per well, 100 μL of cell suspension was added, and the plate was placed in a CO 2 Culture in a culture box; after the cells adhere to the wall, discard the old culture medium, add different concentrations of daylily extract for pretreatment for 1 hour, and then add appropriate concentrations of corticosterone to continue culturing for 24 hours; add an equal amount of CCK-8 reagent to each well, place in a cell culture box and continue culturing for 1-4 hours, and measure the OD value with an enzyme marker at a wavelength of 450nm.

[0110] (4) Enzyme-linked immunosorbent assay (ELISA)

[0111] The 5-HT secretion level was detected by ELISA.

[0112] Table 3

[0113] Cell survival rate (%) 5-HT (ng / L) Example 1 93.24％ 64.01 Example 2 94.38％ 64.14 Example 3 93.67％ 62.98

[0114] From Table 3 and Figure 3The results show that compared with the blank group, the 5-HT content in the model group was significantly reduced; compared with the model group, the four drug-administered groups all increased to varying degrees, among which the HHC medium-dose group had the most significant effect and was better than the positive drug fluoxetine group. Therefore, it is speculated that the daylily extract can regulate monoamine neurotransmitters to achieve the effect of alleviating depression. After corticosterone was administered to damage PC12 cells in Examples 1-3, the cell survival rate increased and approached the normal value; the 5-HT content was significantly higher than that in the model group, indicating that the 5-HT content can be increased after administration. It is speculated that the daylily extract can regulate monoamine neurotransmitters to achieve the purpose of improving depression.

[0115] Finally, it should be noted that in this application, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article or apparatus.

[0116] Although the present application has been disclosed above through the description of the specific embodiments of the present application, it should be understood that those skilled in the art can design various modifications, improvements or equivalents to the present application within the spirit and scope of the attached scheme. These modifications, improvements or equivalents should also be considered to be included in the scope of protection claimed in the present application.

Claims

1. An antidepressant gel candy, characterized in that: The composition comprises the following components by weight: 10-40 parts of edible gum, 40-60 parts of natural sweetener, 0.2-0.8 parts of citric acid, and 5-30 parts of daylily extract; Wherein, the daylily extract is obtained by alcohol extraction of daylily.

2. The antidepressant gel candy according to claim 1, characterized in that: The edible gum includes at least one of gelatin, pectin, carrageenan and xanthan gum; Wherein, when the edible gum includes the gelatin and the pectin, the mass ratio of the gelatin to the pectin is 4:

1.

3. The antidepressant gel candy according to claim 1, characterized in that: The natural sweetener is a sugar alcohol; preferably, the sugar alcohol includes at least one of sorbitol, xylitol, lactitol, mannitol, and maltitol; Wherein, when the sugar alcohol includes the sorbitol and the xylitol, the mass ratio of the sorbitol to the xylitol is 3:

2.

4. The antidepressant gel candy according to claim 1, characterized in that: The preparation method of the daylily extract comprises: crushing the daylily and adding ethanol to extract; Wherein, the particle size of the daylily powder is 200 mesh; Wherein, the mass fraction of the ethanol is 70%; The extraction time is 0.5 to 2 hours, and the extraction times are 2 to 4 times.

5. A method for preparing the antidepressant gel candy as claimed in claim 1, characterized in that: The method comprises the following preparation steps: (1) adding the edible gum into water, soaking and constant temperature water bathing to obtain a swollen edible gum, and setting aside; (2) adding the natural sweetener to the water, dissolving it and then heating it to obtain a sugar solution, which is then cooled and set aside; (3) dissolving the citric acid and the daylily extract in water to obtain a mixture A for later use; (4) After the edible gum swollen product, the sugar solution and the mixture A are stirred, demoulding is performed.

6. The method for preparing the antidepressant gel candy according to claim 5, characterized in that: The preparation method of the antidepressant gel candy meets at least one of the following conditions: In step (1), the mass ratio of the edible gum to the water is 1:(3-6); In step (1), the soaking is done at room temperature; In step (1), the soaking time is 0.5 to 1.5 hours; In step (1), the temperature of the constant temperature water bath is 70° C. In step (1), the constant temperature water bath is kept for 30 minutes.

7. The method for preparing the antidepressant gel candy according to claim 5, characterized in that: The preparation method of the antidepressant gel candy meets at least one of the following conditions: In step (2), the mass ratio of the natural sweetener to the water is 1:(1-3); In step (2), the heating temperature is 90 to 105° C. In step (2), when the sugar solution shows a stringy state during the heating process, the heating is stopped; In step (2), the cooling to 75°C is sufficient.

8. The method for preparing the antidepressant gel candy according to claim 5, characterized in that: In step (3), the mass ratio of the citric acid and the daylily extract to the water is 1:(1-3).

9. The method for preparing the antidepressant gel candy according to claim 5, characterized in that: The preparation method of the antidepressant gel candy meets at least one of the following conditions: In step (4), the stirring time is 0.1-0.5h; In step (4), the stirring speed is 30-60 rpm.

10. The method for preparing the antidepressant gel candy according to claim 5 or 9, characterized in that: Step (4) also includes cooling and demoulding operations.

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