Refined molasses as well as preparation method and application thereof

Abstract

The invention provides refined molasses as well as a preparation method and application thereof, and relates to the technical field of molasses refining. The preparation method of the sugar honey comprises the following steps: S1, dissolving cane molasses in water, and then adding a strain for fermentation to obtain fermented molasses; the strains comprise bacillus subtilis and saccharomyces cerevisiae; s2, adjusting the pH value of the fermented molasses to 8-9, standing, centrifuging, removing lower-layer precipitate, and retaining supernatant; s3, adjusting the pH value of the supernatant to 5.5-7, and performing ultrafiltration and concentration to obtain a concentrated solution; and S4, sterilizing and spray-drying the concentrated solution to obtain the refined molasses. The refined molasses prepared by the method is high in reducing sugar and crude protein content and low in salt and ash content.

Description

Technical Field

[0001] The invention belongs to the technical field of molasses refining, and specifically relates to refined molasses and a preparation method and application thereof. Background Art

[0002] Molasses, also known as syrup or treacle, is a viscous, dark brown, semi-fluid substance that contains a large amount of fermentable sugar (mainly sucrose). It is a good fermentation raw material and can be used as a substrate or base material for fermentation products such as yeast, monosodium glutamate, and organic acids. It can also be used as a raw material for certain foods and animal feed. Depending on the source, molasses can be divided into sugarcane molasses, beet molasses, citrus molasses, and corn molasses.

[0003] Molasses is rarely used in animal husbandry, mainly because its high viscosity makes it difficult to mix feed, and the polysaccharides it contains need to be broken down into reducing sugars during digestion before livestock and poultry can absorb them, which increases the digestive burden. In addition, the crude protein in molasses is mainly non-protein nitrogen, which has a low biological value, and it contains more minerals, especially potassium, magnesium, sodium, chlorine, etc., which may cause diarrhea in livestock and poultry. These factors have jointly limited the application of molasses in animal husbandry production. Summary of the invention

[0004] The present invention overcomes the above technical problems and provides a refined molasses and a preparation method and application thereof. The refined molasses prepared by the present invention has high contents of reducing sugar and crude protein and low salt content.

[0005] The present invention solves the above technical problems through the following technical solutions.

[0006] A method for preparing refined molasses comprises the following steps:

[0007] S1. dissolving sugarcane molasses in water, adding bacteria to ferment, and obtaining fermented molasses;

[0008] The bacterial species include Bacillus subtilis and Saccharomyces cerevisiae;

[0009] S2. The pH of the fermented molasses was adjusted to 8-9, and the mixture was centrifuged after standing, and the lower precipitate was discarded, and the upper liquid was retained;

[0010] S3. The upper liquid is adjusted to pH 5.5-7, and then ultrafiltered and concentrated to obtain a concentrated solution;

[0011] S4. The concentrated liquid is sterilized and spray-dried to obtain refined molasses.

[0012] In S1, the sugarcane molasses contains: 24-36wt% sucrose, 1.5-3wt% crude protein, 12-18% reducing sugar, and 8-10wt% salt; wherein the salt contains potassium ions, sodium ions and magnesium ions, and the reducing sugar includes glucose and fructose.

[0013] In S1, the ash content of the sugarcane molasses is 8.3-9.8%. The increase in ash content will increase the viscosity of the molasses; the greater the ash content in the sugarcane molasses, the greater the corresponding viscosity, and when the ash content decreases, the corresponding viscosity will also decrease.

[0014] In S1, the viscosity of the sugarcane molasses is 6 to 20 Pa·s.

[0015] In S1, the activity of Bacillus subtilis in the strain is > 5×10 7 cfu / mL, preferably 5.2×10 7 ~7.3×10 7 cfu / mL.

[0016] In S1, the activity of Saccharomyces cerevisiae in the strain is > 5×10 9 cfu / mL, preferably 5.8×10 9 ~6.2×10 9 cfu / mL.

[0017] In S1, the temperature of the water is 28-37°C.

[0018] In S1, the mass ratio of the sugarcane molasses to the bacteria is 100:8-10, preferably 100:9-10.

[0019] In S1, the mass ratio of the sugarcane molasses to water is 1:4-10, preferably 1:5-8.

[0020] In S1, the initial pH of the fermentation is controlled at 5.8-6.8, preferably 5.8-6.5.

[0021] In S1, the fermentation temperature is 25-37°C, preferably 28-35°C.

[0022] In S1, the fermentation time is 18 to 24 hours, preferably 20 to 22 hours.

[0023] In S1, the fermentation also requires air to be introduced from the bottom of the fermentation tank, and the flow rate of the fermentation air is 5 to 20 L / min, preferably 7 to 13 L / min.

[0024] In S2, the standing time is 3 to 12 hours.

[0025] In S2, the centrifugal rotation speed is 2000-2800 rpm.

[0026] In S2, the centrifugation time is 3 to 15 minutes.

[0027] In S3, the molecular weight cut-off of the ultrafiltration is 30-100 kDa, preferably 30-50 kDa.

[0028] In S3, the water content after concentration is 40-60%.

[0029] In S4, the spray drying inlet temperature is 140-180°C, and the outlet temperature is 80-90°C.

[0030] In S4, the refined molasses contains: 60-75wt% reducing sugar, 4.8-6.7wt% crude protein, 0.5-1.5wt% salt; preferably, it contains 62-74% reducing sugar, 5.2-6.7% crude protein, and 0.5-1.38wt% salt.

[0031] In S4, the ash content of the refined molasses is 1.76-2.08%.

[0032] In S4, the shelf life of the refined molasses is 6 to 9 months. The refining process of the present invention includes drying granulation and sterilization operations, which is more conducive to the preservation of the refined molasses.

[0033] In S4, the refined molasses is solid, preferably in the form of blocks, granules or powder.

[0034] The application of the refined molasses as mentioned above is in chicken feed, ruminant feed and pig feed;

[0035] Among them, the chicken feed includes breeding laying hen feed and fattening broiler feed; the ruminant feed includes cattle feed and sheep feed.

[0036] Further, the refined molasses is added to replace 2-5% of chicken feed;

[0037] Furthermore, the refined molasses is added to replace 3-8% of ruminant feed;

[0038] Furthermore, the refined molasses is added to replace 3-8% of pig feed;

[0039] Furthermore, the refined molasses is dissolved in water and directly provided to chickens, ruminants or pigs for drinking, wherein the added amount of the refined molasses is 2-5% of the mass of water.

[0040] On the basis of being in accordance with the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present invention.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] 1. The present invention degrades polysaccharides into reducing monosaccharides that are easy for animals to digest and absorb by fermenting bacteria. This fermentation process not only promotes the animal's nutrient absorption, but also effectively removes the salt in the sugarcane molasses by consuming the excess salt in the molasses. It can also avoid the problem of diarrhea in the animals caused by excessive sodium, magnesium and potassium ions in the sugarcane molasses.

[0043] 2. After fermentation and refining, the content of polysaccharides, metal ions and colloidal substances in molasses is significantly reduced, and these components are the main reasons for the viscosity of molasses. At the same time, this process can also effectively remove the non-protein nitrogen content in crude protein. In some preferred embodiments, the refined molasses contains 60-75wt% reducing sugars, 4.8-6.7wt% crude protein, 0.5-1.5wt% salt, and 1.76-2.08wt% ash; in some preferred embodiments, the refined molasses contains 62-74wt% reducing sugars, 5.2-6.7wt% crude protein, and 0.5-1.38wt% salt; the proportion adjustment of these components helps to improve the nutritional value and application effect of molasses.

[0044] 3. The refined molasses fermentation method of the present invention is capable of converting the originally viscous sugarcane molasses, which is characterized by high water content, high polysaccharide, salt and ash content, into a solid granular product. This conversion significantly simplifies the transportation and storage process of molasses and effectively solves the problem of molasses deterioration that may occur during these processes. By reducing the water content and lowering the polysaccharide, salt and ash content, the physical state of the molasses is improved, thereby improving its stability during storage and transportation.

[0045] 4. The refined molasses of the present invention can be used as a substitute for feed additives and added to the diet of animals due to its sweet smell and strong appetizing property. In addition, the feeding of refined molasses can also bring obvious health benefits to animals with intestinal dysplasia or animals that have not yet fully recovered from illness. By providing nutrients that are easily digestible and absorbable, refined molasses helps the intestinal health and recovery of these animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 Thin layer chromatograms of cane molasses and refined molasses. DETAILED DESCRIPTION

[0047] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and carefully in combination with preferred embodiments below, but the protection scope of the present invention is not limited to the following specific embodiments.

[0048] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0049] The "range" disclosed in the present invention is defined in the form of a lower limit and an upper limit, and a given range is defined by selecting a lower limit and an upper limit, and the selected lower limit and upper limit define the boundaries of a particular range. The range defined in this way can be inclusive or exclusive of end values, and can be combined arbitrarily, that is, any lower limit can be combined with any upper limit to form a range. For example, if a range of 60-120 and 80-110 is listed for a specific parameter, it is understood that the range of 60-110 and 80-120 is also expected. In addition, if the minimum range values ​​1 and 2 are listed, and if the maximum range values ​​3, 4 and 5 are listed, the following ranges can all be expected: 1-3, 1-4, 1-5, 2-3, 2-4 and 2-5. In the present invention, unless otherwise specified, the numerical range "ab" represents an abbreviation of any real number combination between a and b, wherein a and b are real numbers. For example, the numerical range "0-5" represents that all real numbers between "0-5" have been fully listed herein, and "0-5" is just an abbreviation of these numerical combinations. In addition, when a parameter is expressed as an integer ≥ 2, it is equivalent to disclosing that the parameter is, for example, an integer of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0050] If not otherwise specified, all embodiments and optional embodiments of the present invention can be combined with each other to form a new technical solution.

[0051] Unless otherwise specified, all technical features and optional technical features of the present invention can be combined with each other to form a new technical solution.

[0052] If not otherwise specified, all steps of the present invention may be performed sequentially or randomly, preferably sequentially. For example, the method includes steps (a) and (b), which means that the method may include steps (a) and (b) performed sequentially, or may include steps (b) and (a) performed sequentially. For example, the method may further include step (c), which means that step (c) may be added to the method in any order, for example, the method may include steps (a), (b) and (c), or may include steps (a), (c) and (b), or may include steps (c), (a) and (b), etc.

[0053] If there is no special explanation, the "include" and "comprising" mentioned in the present invention represent open-ended or closed-ended expressions. For example, the "include" and "comprising" may represent that other components not listed may also be included or only the listed components may be included or only the listed components may be included.

[0054] If not specifically stated, in the present invention, the term "or" is inclusive. For example, the phrase "A or B" means "A, B, or both A and B". More specifically, any of the following conditions satisfies the condition "A or B": A is true (or exists) and B is false (or does not exist); A is false (or does not exist) and B is true (or exists); or both A and B are true (or exist).

[0055] The sugarcane molasses used in the following examples or comparative examples contained 33% sucrose, 30% water, 1.8% crude protein, 14% reducing sugar and 9% salt; Bacillus subtilis was purchased from Leshengyuan Biotechnology (Nanjing) Co., Ltd., and Saccharomyces cerevisiae was purchased from Tianjin Yunlizhixing Biotechnology Co., Ltd.

[0056] Example 1

[0057] The preparation method of the refined molasses of the present embodiment comprises the following steps:

[0058] S1. 50 kg of sugarcane molasses was dissolved in 35 ° C water, mixed and sterilized, and then the bacteria were added. The initial pH of the fermentation was 6.4, and the fermentation temperature was controlled at 28-30 ° C. The fermentation also required air to be introduced from the bottom of the fermentation tank at an air flow rate of 7 L / min. After stirring and fermenting for 20 hours, the fermented molasses was obtained;

[0059] In this embodiment, the strain is a 0.5wt% bacterial solution, and the strain is prepared by mixing LB culture medium powder (purchased from Beijing Yita Biotechnology Co., Ltd.) and water at a mass ratio of 10:1 and sterilizing, and then fermenting and inoculating at 30°C with stirring (200rpm) for 24 hours to obtain the strain. Among them, the strain activity can be controlled by adjusting the fermentation inoculation time, and the inoculation fermentation time can be 12 to 48 hours.

[0060] In this example, the activity of Bacillus subtilis in the strain was 5.2×10 7 cfu / mL, the activity of Saccharomyces cerevisiae was 6.2×10 9 cfu / mL;

[0061] In this embodiment, the mass ratio of sugar cane molasses to bacterial strain is 100:9; the mass ratio of sugar cane molasses to water is 1:8.

[0062] S2. The pH of the fermented molasses was adjusted to 9, allowed to stand for 4 h, centrifuged at 2500 rpm for 10 min, the lower precipitate was discarded, and the upper liquid was retained;

[0063] S3. The upper layer was adjusted to pH 6.0, and then ultrafiltration with a molecular weight cutoff of 30 kDa was performed and concentrated to a concentrate with a water content of 43%;

[0064] S4. The concentrate is sterilized and spray dried. The spray drying air inlet temperature is 150°C and the air outlet temperature is 80°C to obtain refined molasses. The thin layer chromatograms of refined molasses and cane molasses are shown in Figure 1. Figure 1 ,from Figure 1 It can be seen that sugarcane molasses has a high sucrose content and a low reducing sugar content, while refined molasses has a high reducing sugar content and does not contain sucrose; in addition, the gray color at the bottom of the thin layer chromatography plate of sugarcane molasses is salt, while refined molasses has a low salt content.

[0065] Example 2

[0066] In this example, the activity of Bacillus subtilis in the strain was 6.2×10 7 cfu / mL, Saccharomyces cerevisiae activity 6.2×10 9 cfu / mL, and other steps and parameters were the same as in Example 1.

[0067] Example 3

[0068] In this embodiment, the mass ratio of sugarcane molasses to bacteria is 100:10, and other steps and parameters are the same as those in Example 1.

[0069] Example 4

[0070] In this example, the initial pH of the fermentation was 5.8, and the other steps and parameters were the same as those in Example 1.

[0071] Example 5

[0072] In this example, the initial pH of the fermentation was 6.0, and the other steps and parameters were the same as those in Example 1.

[0073] Example 6

[0074] In this example, the initial pH of the fermentation was 6.2, and the other steps and parameters were the same as those in Example 1.

[0075] Example 7

[0076] In this example, the fermentation temperature was controlled at 32-35° C., and the other steps and parameters were the same as those in Example 1.

[0077] Example 8

[0078] In this example, the fermentation time is 18 h, and the other steps and parameters are the same as those in Example 1.

[0079] Example 9

[0080] In this example, the fermentation time is 22 hours, and the other steps and parameters are the same as those in Example 1.

[0081] Example 10

[0082] In this example, the fermentation time is 24 hours, and the other steps and parameters are the same as those in Example 1.

[0083] Embodiment 11

[0084] In this example, the air flow rate is 8 L / min, and the other steps and parameters are the same as those in Example 1.

[0085] Example 12

[0086] In this example, the air flow rate is 9 L / min, and the other steps and parameters are the same as those in Example 1.

[0087] Example 13

[0088] In this example, 50 kg of sugarcane molasses was dissolved in 28°C water;

[0089] In this example, the initial pH of the fermentation was 5.2;

[0090] In this embodiment, the fermentation time is 22h;

[0091] In this embodiment, the air flow rate is 8L / min;

[0092] Other steps and parameters are the same as in Example 1.

[0093] Comparative Example 1

[0094] In this comparative example, the activity of Bacillus subtilis in the strain was 9.2×10 7 cfu / mL, Saccharomyces cerevisiae activity 8.0×10 9 cfu / mL, and other steps and parameters were the same as in Example 1.

[0095] Comparative Example 2

[0096] In this comparative example, the mass ratio of sugarcane molasses to bacteria is 100:7, and other steps and parameters are the same as those in Example 1.

[0097] Comparative Example 3

[0098] In this comparative example, the mass ratio of sugarcane molasses to bacteria was 100:11, and other steps and parameters were the same as those in Example 1.

[0099] Comparative Example 4

[0100] In this comparative example, the initial pH of the fermentation was 5.5, and the other steps and parameters were the same as those in Example 1.

[0101] Comparative Example 5

[0102] In this comparative example, the fermentation temperature was controlled at 23-24° C., and the other steps and parameters were the same as those in Example 1.

[0103] Comparative Example 6

[0104] In this example, the fermentation time is 26 hours, and the other steps and parameters are the same as those in Example 1.

[0105] Comparative Example 7

[0106] In this comparative example, the molecular weight cut-off is 10 kDa, and the other steps and parameters are the same as those in Example 1.

[0107] Test Example 1

[0108] The results of the test of the above examples and comparative examples are shown in Table 1.

[0109] Table 1

[0110] - Reducing sugar / % Crude protein / % Salt / % Ash / % Example 1 67 6.2 0.62 1.95 Example 2 61 6.1 0.83 1.82 Example 3 69 6.3 0.58 1.78 Example 4 62 6.2 1.28 1.85 Example 5 74 6.7 0.89 1.79 Example 6 60 6.4 1.38 1.93 Example 7 63 5.4 0.96 1.90 Example 8 68 5.2 0.51 1.81 Example 9 73 5.6 0.35 1.77 Example 10 70 4.9 0.63 1.99 Embodiment 11 71 5.1 0.82 1.85 Example 12 66 5.3 1.33 2.06 Example 13 69 6.5 0.79 2.03 Comparative Example 1 52 6.5 1.78 2.56 Comparative Example 2 45 4.8 1.64 5.72 Comparative Example 3 48 4.3 0.94 2.11 Comparative Example 4 58 5.1 1.66 2.47 Comparative Example 5 50 4.6 2.35 3.22 Comparative Example 6 54 4.2 0.49 2.67 Comparative Example 7 53 3.6 0.56 1.34

[0111] Crude protein test method: GB / T 6432.

[0112] Salt content test method: GB / T 13885.

[0113] Ash content test method: GB / T 6438.

[0114] The test method for reducing sugar (measured in glucose) is as follows:

[0115] (1) 1 mg / ml glucose standard solution: Accurately weigh 100 mg of dry constant weight glucose, add a small amount of distilled water to dissolve it, and then make up to 100 ml with distilled water. This means that the glucose content is 1.0 mg / ml.

[0116] (2) 3,5-Dinitrosalicylic acid reagent: Weigh 6.3 g of 3,5-dinitrosalicylic acid and 262 ml of 2 mol / L NaOH and add them to a hot solution of potassium sodium tartrate (182 g of potassium sodium tartrate is dissolved in 500 ml of water). Then add 5 g of crystalline phenol and 5 g of sodium bisulfite and dissolve them therein. Stir to dissolve. After cooling, adjust the volume to 1000 ml and store in a brown bottle.

[0117] (3) Equipment: spectrophotometer, balance, water bath, electric furnace, test tubes, etc.

[0118] (4) Draw a glucose standard curve

[0119] Take 8 15mm×180mm test tubes, add 1.0mg / ml glucose standard solution and distilled water according to Table 2, mix the solutions in each tube evenly, heat in boiling water for 5min, cool to room temperature with cold water immediately after taking out, then add 21.5ml distilled water to each tube and shake well. Measure the absorbance at λ=540nm, draw a standard curve with glucose content (mg) as the horizontal axis and light absorption value as the vertical axis.

[0120] Table 2

[0121]

[0122] (5) Sample preparation

[0123] Accurately weigh 0.5g of the refined molasses obtained in the above examples or comparative examples, put it in a 100ml beaker, first add a small amount of distilled water (about 2ml) to make a paste, then add 40ml of distilled water, mix well, keep warm in a 50℃ constant temperature water bath for 20min, stir from time to time to make the reducing sugar leached out of the mixture. Filter, collect all the filtrate in a 50ml volumetric flask, and dilute to the mark with distilled water to obtain the sample.

[0124] (6) Determination of reducing sugar in samples

[0125] Mix the solutions in each tube evenly, heat them in boiling water for 5 minutes, cool them to room temperature with cold water immediately after taking them out, then add 21.5 ml of distilled water to each tube and shake them well. Measure the absorbance at λ = 540 nm. After the reducing sugar in the sample solution is determined, take the light absorption value of the sample and find the corresponding reducing sugar content on the standard curve. Calculate the percentage of reducing sugar and total sugar in the sample according to the following formula: Reducing sugar (in terms of glucose) % = C × V / m × 1000C;

[0126] In the formula: the concentration of reducing sugar extract, mg / ml; V - the total volume of reducing sugar extract, ml; m - sample weight, g; 1000 - the coefficient for converting mg to g.

[0127] Application Examples

[0128] In the application experiment conducted in a large beef cattle farm, 60 beef cattle weighing 382.7±5.68kg were selected. The experimental design adopted a 10×3 block design, that is, the experimental group and the control group each had three replicates, and each replicate contained 10 beef cattle. The beef cattle in the control group were fed only the basic feed, while the beef cattle in the experimental group were fed with 5% refined molasses (prepared according to Example 13) in the basic feed to replace corn. The specific composition of the basic concentrate is shown in Table 3.

[0129] Table 3

[0130]

[0131]

[0132] The pre-feeding period of the experiment was 15 days and the experimental period was 45 days, and the daily feed intake data of each beef cattle was recorded. After the experiment, the average daily feed intake of the experimental group and the control group was calculated, and the specific data are shown in Table 4.

[0133] Table 4

[0134] - Experimental Group Control group Feed intake / (kg / day) 11.3 14.6

[0135] In livestock and poultry breeding, the use of refined molasses can also promote intestinal development, improve intestinal health, and increase feed digestibility. This not only helps the growth and development of animals, but also improves overall feeding efficiency.

[0136] In addition, using refined molasses as a feed substitute can also bring the following benefits: improve the feces condition of the animals and reduce the problem of loose stools; reduce the amount of feces and reduce the odor in the breeding house, thereby improving the breeding environment. For animals with poor intestinal development or those who have not fully recovered from illness, the use of refined molasses has a significant positive effect and can promote their recovery. At the same time, refined molasses can also increase the survival rate of small animals, bringing better economic benefits to breeders.

[0137] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods. The specific embodiments described above further describe the purpose, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing refined molasses, characterized in that: The following steps are involved: S1. dissolving sugarcane molasses in water, adding bacteria to ferment, and obtaining fermented molasses; The bacterial species include Bacillus subtilis and Saccharomyces cerevisiae; The initial pH of the fermentation is controlled at 5.8 to 6.8; S2. The pH of the fermented molasses was adjusted to 8-9, and the mixture was centrifuged after standing, and the lower precipitate was discarded, and the upper liquid was retained; S3. The upper liquid is adjusted to pH 5.5-7, and then ultrafiltered and concentrated to obtain a concentrated solution; S4. The concentrated liquid is sterilized and spray-dried to obtain refined molasses.

2. The method for preparing refined molasses according to claim 1, characterized in that: The sugarcane molasses comprises: 24-36 wt% sucrose, 1.5-3 wt% crude protein, 12-18% reducing sugar, and 8-10 wt% salt; wherein the salt comprises potassium ions, sodium ions and magnesium ions, and the reducing sugar comprises glucose and fructose; And / or, the ash content of the sugarcane molasses is 8.3-9.8%.

3. The method for preparing refined molasses according to claim 1, characterized in that: The activity of Bacillus subtilis in the strain is > 5×10 7 cfu / mL; And / or, the activity of Saccharomyces cerevisiae in the strain is greater than 5×10 9 cfu / mL.

4. The method for preparing refined molasses as claimed in claim 1, characterized in that The mass ratio of the sugarcane molasses to the bacterial strain is 100:8-10; And / or, the mass ratio of the sugarcane molasses to water is 1:4-10.

5. The method for preparing refined molasses according to claim 1, characterized in that: The fermentation temperature is 25-37°C; And / or, the fermentation time is 18 to 24 hours; And / or, the flow rate of the fermentation air is 5 to 20 L / min.

6. The method for preparing refined molasses according to claim 1, characterized in that: The ultrafiltration molecular weight cut-off is 30 to 100 kDa; And / or, the water content after concentration is 40-60%.

7. The method for preparing refined molasses according to claim 1, characterized in that: The spray drying inlet temperature is 140-180°C, and the outlet temperature is 80-90°C.

8. The refined molasses obtained by the method for preparing refined molasses according to any one of claims 1 to 7, characterized in that: The refined molasses contains: 60-75% reducing sugar, 4.8-6.7wt% crude protein, and 0.5-1.5wt% salt; And / or, the ash content of the refined molasses is 1.76-2.08%.

9. The use of refined molasses as claimed in claim 8, characterized in that: The application is application in chicken feed, ruminant feed and pig feed; Wherein, the chicken feed includes breeding type laying hen feed and fattening type broiler feed; The ruminant feed includes cattle feed and sheep feed.

10. The use of refined molasses according to claim 9, characterized in that: Satisfy at least one of the following conditions ① to ④: ① The refined molasses is added to replace 2-5% of chicken feed; ② The refined molasses is added to replace 3-8% of ruminant feed; ③ The refined molasses is added to replace 3-8% of pig feed; ④ The refined molasses is dissolved in water and directly provided to chickens, ruminants or pigs for drinking, wherein the added amount of the refined molasses is 2-5% of the mass of water.

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