Anti-CD28 nano antibody as well as preparation method and application thereof

Through the combination of phage display technology and high-throughput mammalian cell expression system, high-sensitivity and specific anti-CD28 nano-antibodies were screened, solving the problems of high cost and low efficiency of CD28 antibodies in the prior art, and achieving the following: Efficient application in tumor treatment and detection.

CN119285779AActive Publication Date: 2025-01-10BIOINTRON BIOLOGICAL INC
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Patent Information

Application Number
CN202411576074.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-11-20
Publication Date
2025-01-10
Estimated Expiration
2043-11-20

AI Technical Summary

Technical Problem

The existing CD28 antibodies have high development and production costs and low efficiency in tumor treatment and detection, and it is difficult to effectively bind to target cells, affecting the therapeutic effect.

Method used

Through phage display technology combined with high-throughput mammalian cell expression system, anti-CD28 nano-antibody with high sensitivity and specificity is screened and obtained. Cell panning flow sorting technology is used to reduce non-specific binding, reduce production costs and improve efficiency .

Benefits of technology

It has achieved efficient and low-cost development and production of anti-CD28 nanoantibodies, which has improved the specificity and sensitivity of the antibodies and enhanced its application value in tumor treatment and detection.

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to an anti-CD28 nano antibody as well as a preparation method and application thereof. The preparation process of the anti-CD28 nano antibody provided by the invention comprises the following steps: constructing a CD28 antigen to immunize alpaca, performing phage library construction on the immunized alpaca PBMC, screening the phage library, and selecting a correct sequence to construct, express and purify; and finally obtaining the anti-CD28 nano antibody through cell function detection and screening. The nano antibody provided by the invention has specific recognition and binding capacity to CD28, and has the advantages of strong specificity, high sensitivity and the like; meanwhile, the development and production cost of the CD28 antibody is effectively reduced, and the antibody expression time is shortened.
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Citation Information

Patent Citations

  • Anti-CD28 nano antibody, coding gene and application

    CN114605540A

  • Anti-CD28 antibody and application thereof

    CN116514974A

  • Anti-human CD28 engineering antibody and application

    CN116813783A

Cited By

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    CN121554587A

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