Soil conditioner for improving stability of organic matters in brick red soil and application of soil conditioner

Abstract

The invention relates to a soil conditioner for improving stability of organic matters in brick red soil and application of the soil conditioner. The soil conditioner comprises microalgae, humic acid or derivatives thereof. The microalgae comprise scenedesmus and / or chlorella. According to the soil conditioner developed by the invention, scenedesmus and / or chlorella are / is taken as a main raw material, humic acid or a derivative thereof is taken as an auxiliary material, and the scenedesmus and the chlorella are matched together, so that the soil conditioner has excellent effects of promoting formation of soil microaggregates and promoting formation of large aggregates, and further has an excellent effect of improving the stability of brick red soil organic matters.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture and relates to a soil conditioner for improving the stability of organic matter in brick red soil and application thereof. Background Art

[0002] Brick red soil is a deep red soil developed by strong iron-aluminization and biological enrichment. It is named after the color of the soil, which is similar to burned red bricks. The distribution area of ​​brick red soil has excellent water and heat conditions and has always been an important crop production area in southern my country. However, the organic matter content of most brick red soils is about 1%, and the low organic matter content limits the development and utilization of brick red soil.

[0003] Organic matter is the core component of soil, and is of great significance for regulating soil fertility, improving soil structure, and regulating soil carbon sinks. Organic matter is centered on organic carbon, and improving the stability of organic matter focuses on improving the stability of soil organic carbon. Excellent soil aggregate structure can play a good role in protecting and stabilizing soil organic carbon. However, brick red soil is a soil with strong iron-aluminum-rich characteristics and poor soil aggregate structure. It is necessary to use technical means to promote the formation of brick red soil aggregates (including small, large, and super-large aggregates, etc.). Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a soil conditioner for improving the stability of organic matter in brick red soil and its application, and specifically to provide a soil conditioner for improving the stability of organic matter in brick red soil and its application in improving the aggregate structure of brick red soil and / or improving the stability of organic matter in brick red soil.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a soil conditioner for improving the stability of organic matter in lateosoil, wherein the soil conditioner comprises microalgae, humic acid or its derivatives; and the microalgae comprises Scenedesmus and / or Chlorella.

[0007] The soil conditioner developed by the present invention uses Scenedesmus and / or Chlorella as main raw materials and humic acid or its derivatives as auxiliary materials. The two are combined to have excellent effects of promoting the formation of soil microaggregates and promoting the formation of macroaggregates, and further has an excellent effect of improving the stability of organic matter in brick red soil.

[0008] Preferably, the humic acid or its derivatives include any one of potassium humate, potassium nitrohumate or potassium fulvic acid, or a combination of at least two thereof, preferably potassium humate.

[0009] Compared with other types of humic acid or its derivatives, potassium humate combined with microalgae has a better effect in promoting soil aggregate formation and stabilizing organic matter.

[0010] Preferably, the Scenedesmus is a Scenedesmus with a deposit number of GDMCC No. 63703, which is named Scenedesmus sp. CJ and is deposited in Guangdong Microbial Culture Collection Center.

[0011] Preferably, the Chlorella is the Chlorella with a preservation number of GDMCC No. 63704, which is named Chlorella sp. ZJ and is preserved in Guangdong Provincial Microbial Culture Collection Center.

[0012] Preferably, the microalgae are in the form of microalgae liquid or microalgae powder.

[0013] Preferably, the amount of microalgae used is per gram of humic acid or its derivatives, the number of microalgae cells is (1×10 5 )-(1×10 7 ) pieces, for example (1×10 5 )pcs, (5×10 5 )、(8×10 5 ) pieces, (1×10 6 )pcs, (5×10 6 )、(8×10 6 ) pieces, (1×10 7 ) etc. Other specific point values ​​within this numerical range can be selected and will not be described in detail here.

[0014] Preferably, the microalgae liquid is prepared by a method comprising:

[0015] The microalgae are inoculated into a culture medium and cultured under light.

[0016] Preferably, the culture temperature is 25-35°C, for example, 25°C, 26°C, 27°C, 28°C, 29°C, 30°C, 31°C, 32°C, 33°C, 34°C, 35°C, etc.

[0017] Preferably, the intensity of the illumination is 2000-12000 Lux, for example, 2000 Lux, 4000 Lux, 5000 Lux, 6000 Lux, 7000 Lux, 8000 Lux, 10000 Lux, 12000 Lux, etc.

[0018] Preferably, the culture medium is BG-11 medium.

[0019] The formula of the BG-11 culture medium includes: NaNO3 0.5-1.5g / L, K2HPO4 0.02-0.06g / L, MgSO4·7H2O 0.05-0.1g / L, CaCl2·2H2O 0.01-0.05g / L, citric acid 0.002-0.01g / L, ammonium ferric citrate 0.002-0.01g / L, EDTA 0.0005-0.001g / L, Na2CO3 0.01-0.03g / L, H3BO4 0.001-0.005g / L, MnCl2·H2O 0.001-0.003g / L, ZnSO4·7H2O 0.0001-0.0005g / L, CuSO4·5H2O 0.00005-0.0001g / L, Na2MoO4·2H2O 0.0001-0.0005g / L, Co(NO3)2·6H2O 0.00002-0.00008g / L; the solvent is deionized water.

[0020] Preferably, the culturing is performed until the microalgae cell concentration reaches (1×10 7 )-(5×10 7 ) / mL were collected, for example (1×10 7 ) / mL, (2×10 7 ) / mL, (3×10 7 ) / mL, (4×10 7 ) / mL, (5×10 7 )pcs / mL, etc.

[0021] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0022] Preferably, the mass ratio of the microalgae liquid to humic acid or its derivatives is (700-900):(30-100).

[0023] The specific point values ​​in (700-900) can be selected from 700, 720, 750, 780, 800, 820, 850, 900, etc.; the specific point values ​​in (30-100) can be selected from 30, 40, 50, 60, 70, 80, 90, 100, etc.; other specific point values ​​within this numerical range can be selected, and they will not be listed here one by one.

[0024] In a second aspect, the present invention provides use of the soil conditioner according to the first aspect in improving the aggregate structure of red soil.

[0025] Preferably, in the above application, the soil conditioner is used by diluting the soil conditioner with water and then spraying it on the soil surface.

[0026] In a third aspect, the present invention provides use of the soil conditioner according to the first aspect in improving the stability of organic matter in red soil.

[0027] Preferably, in the above application, the soil conditioner is used by diluting the soil conditioner with water and then spraying it on the soil surface.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The soil conditioner developed by the present invention uses Scenedesmus and / or Chlorella as main raw materials and humic acid or its derivatives as auxiliary materials. The two are combined to have excellent effects of promoting the formation of soil microaggregates and promoting the formation of macroaggregates, and further has an excellent effect of improving the stability of organic matter in brick red soil. DETAILED DESCRIPTION

[0030] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0031] The Scenedesmus involved in the following content is the Scenedesmus with a preservation number of GDMCC No.63703, named Scenedesmus sp.CJ, and preserved in Guangdong Provincial Microbiological Culture Collection Center. The preservation date is August 1, 2023, and the preservation address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0032] The Chlorella involved in the following content is the Chlorella with the preservation number GDMCC No.63704, named Chlorella sp.ZJ, preserved in Guangdong Provincial Microbiological Culture Collection Center, the preservation time is August 1, 2023, and the preservation address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0033] Example 1

[0034] This embodiment provides a soil conditioner, and the preparation method thereof is as follows:

[0035] (1) Preparation of microalgae solution: Scenedesmus was collected from the microalgae culture greenhouse of Zhanjiang Biomass Power Plant of Guangdong Energy Group, inoculated into BG-11 liquid culture medium at 5%, and cultured at 30°C and 4000 Lux with flue gas from the power plant as the carbon source. The absorbance of the culture solution was measured by a spectrophotometer, and the absorbance of the culture solution was used to reflect the biomass of the microalgae. When the concentration of microalgae cells reached 2×10 7 When the concentration of microalgae was 0.1447 / mL, the microalgae liquid was harvested.

[0036] The composition of BG-11 liquid culture medium is as follows: NaNO3 1.00 g / L, K2HPO4 0.04 g / L, MgSO4·7H2O0.075 g / L, CaCl2·2H2O 0.036 g / L, citric acid 0.006 g / L, ammonium ferric citrate 0.006 g / L, EDTA 0.001 g / L, Na2CO3 0.02 g / L, H3BO4 0.00286 g / L, MnCl2·H2O0.00181 g / L, ZnSO4·7H2O 0.000222 g / L, CuSO4·5H2O 0.000079 g / L, Na2MoO4·2H2O 0.00039 g / L, Co(NO3)2·6H2O 0.000049g / L; the solvent is deionized water.

[0037] (2) Preparation of soil conditioner: Add potassium humate to the microalgae liquid and stir thoroughly. The mass ratio of potassium humate to microalgae liquid is 800:70. The soil conditioner is obtained after all the humate is dissolved.

[0038] Example 2

[0039] This embodiment provides a soil conditioner, and the preparation method thereof is as follows:

[0040] (1) Preparation of microalgae solution: Chlorella vulgaris was collected from the microalgae culture greenhouse of the Zhanjiang Biomass Power Plant of Guangdong Energy Group, inoculated into BG-11 liquid culture medium at 5%, and cultured at 35°C and 8000 Lux with flue gas from the power plant as the carbon source. The absorbance of the culture solution was measured by a spectrophotometer, and the absorbance of the culture solution was used to reflect the biomass of the microalgae. When the concentration of microalgae cells reached 3×10 7 When the concentration of microalgae was 0.1447 / mL, the microalgae liquid was harvested.

[0041] The composition of BG-11 liquid culture medium is as follows: NaNO3 1.00 g / L, K2HPO4 0.04 g / L, MgSO4·7H2O0.075 g / L, CaCl2·2H2O 0.036 g / L, citric acid 0.006 g / L, ammonium ferric citrate 0.006 g / L, EDTA 0.001 g / L, Na2CO3 0.02 g / L, H3BO4 0.00286 g / L, MnCl2·H2O0.00181 g / L, ZnSO4·7H2O 0.000222 g / L, CuSO4·5H2O 0.000079 g / L, Na2MoO4·2H2O 0.00039 g / L, Co(NO3)2·6H2O 0.000049g / L; the solvent is deionized water.

[0042] (2) Preparation of soil conditioner: Add potassium humate to the above microalgae liquid and stir thoroughly. The mass ratio of potassium humate to microalgae liquid is 900:100. The soil conditioner is obtained after all the humate is dissolved.

[0043] Example 3

[0044] This embodiment provides a soil conditioner, and the preparation method thereof is as follows:

[0045] (1) Preparation of microalgae solution: Chlorella vulgaris was collected from the microalgae culture greenhouse of Zhanjiang Biomass Power Plant of Guangdong Energy Group, inoculated into BG-11 liquid culture medium at 5%, and cultured at 25°C and 2000 Lux with flue gas from the power plant as the carbon source. The absorbance of the culture solution was measured by a spectrophotometer, and the absorbance of the culture solution was used to reflect the biomass of the microalgae. When the concentration of microalgae cells reached 1×10 7 When the concentration of microalgae was 0.1447 / mL, the microalgae liquid was harvested.

[0046] The composition of BG-11 liquid culture medium is as follows: NaNO3 1.00 g / L, K2HPO4 0.04 g / L, MgSO4·7H2O0.075 g / L, CaCl2·2H2O 0.036 g / L, citric acid 0.006 g / L, ammonium ferric citrate 0.006 g / L, EDTA 0.001 g / L, Na2CO3 0.02 g / L, H3BO4 0.00286 g / L, MnCl2·H2O0.00181 g / L, ZnSO4·7H2O 0.000222 g / L, CuSO4·5H2O 0.000079 g / L, Na2MoO4·2H2O 0.00039 g / L, Co(NO3)2·6H2O 0.000049g / L; the solvent is deionized water.

[0047] (2) Preparation of soil conditioner: Add potassium humate to the microalgae liquid and stir thoroughly. The mass ratio of potassium humate to microalgae liquid is 700:50. The soil conditioner is obtained after all the humate is dissolved.

[0048] Example 4

[0049] This embodiment provides a soil conditioner, and the preparation method thereof is different from that of Embodiment 1 only in that potassium humate is replaced with potassium fulvic acid, and other conditions remain unchanged.

[0050] Example 5

[0051] This embodiment provides a soil conditioner, and the preparation method thereof is different from that of Embodiment 1 only in that potassium humate is replaced with potassium nitrohumate, and other conditions remain unchanged.

[0052] Example 6

[0053] This embodiment provides a soil conditioner, and the preparation method thereof is different from that of embodiment 1 only in that the preparation method of the microalgae solution in step (1) is different: Scenedesmus and Chlorella are collected from the microalgae culture light greenhouse of Zhanjiang Biomass Power Plant of Guangdong Energy Group, and Scenedesmus and Chlorella are inoculated into BG-11 liquid culture medium at 3% and 3%, respectively, and the flue gas of the power plant is used as a carbon source. The culture is carried out at 30°C and a light intensity of 4000 Lux, and the absorbance value of the culture solution is measured by a spectrophotometer. The absorbance value of the culture solution reflects the biomass of the microalgae. When the concentration of the microalgae cells reaches 2×10 7 When the concentration of microalgae was 0.1447 / mL, the microalgae solution was harvested, and other conditions remained unchanged.

[0054] Example 7

[0055] This embodiment provides a soil conditioner, and the preparation method thereof is different from that of Embodiment 1 only in that Scenedesmus is replaced by Chlorella, and other conditions remain unchanged.

[0056] Comparative Example 1

[0057] The microalgae liquid prepared in step (1) of Example 1 is used as the product of Comparative Example 1.

[0058] Comparative Example 2

[0059] Potassium humate alone was used as the product of Comparative Example 2.

[0060] Test Example 1

[0061] (1) Soil sample collection: Soil samples (typical brick red soil) were collected from the field plots of the experimental base of the Zhanjiang Research Center of the South China Seed Research Institute of the Guangdong Academy of Sciences. After removing stones and plant roots, the soil samples were air-dried and passed through a 0.25 mm sieve for later use.

[0062] (2) Grouping: 2.1) blank control (only water added), 2.2) treatment with the conditioning agent prepared in Examples 1 and 4-7 (0.5 g / kg soil), 2.3) treatment with the microalgae solution of Comparative Example 1 only (0.46 g / kg soil), 2.4) treatment with potassium humate of Comparative Example 2 only (0.04 g / kg soil).

[0063] (3) Experimental method: Prepare several culture pots, take 1 kg of the soil sample prepared in step (1) in each pot, and add the conditioner, microalgae solution, and potassium humate diluted with clean water in proportion according to the treatment settings of (2) above, and add only clean water to the blank control. The amount of liquid added to each pot is controlled at 50 mL. Each treatment is repeated 3 times. The soil of each treatment is placed in a closed culture tank for 30 days. Deionized water is used to control the soil moisture at 70% of the field water holding capacity during the entire experiment.

[0064] On the 3rd, 5th, 10th, 15th and 30th days after the start of the experiment, the gas at the top of the culture tank was collected, and the CO2 content in the gas was determined by titration, and the cumulative soil respiration (g C / kg dry soil) was calculated. The cumulative soil respiration was used to characterize the stability of soil organic matter. Destructive sampling was carried out after 30 days: the content of super-large aggregates (>2mm), large aggregates (1-2mm) and small aggregates (0.25-1mm) was determined by wet sieving; the soil samples were ultrasonically treated and passed through a 53μm sieve. The content of particulate organic matter components (>53μm) and mineral-bound organic matter components (<53μm) were determined by H2SO4-K2Cr2O7 redox method, and each indicator was measured 5 times in parallel. The results are shown in Table 1.

[0065] Table 1

[0066]

[0067] As can be seen from the data results in Table 1, compared with the treatment method of microalgae liquid alone or potassium humate alone, the soil conditioner involved in the present invention has a significantly excellent effect of promoting soil aggregate formation and improving the stability of brick red soil organic matter. By comparing Example 1 with Examples 4-5, it is shown that compared with other types of humic acid or its derivatives, potassium humate and microalgae have a better effect of promoting soil aggregate formation and stabilizing organic matter. By comparing Example 1 with Examples 6-7, it is shown that the effect of using Scenedesmus and Chlorella together is better than using Scenedesmus alone or Chlorella alone.

[0068] Test Example 2

[0069] (1) Soil sample collection: Soil samples (typical brick red soil) were collected from the Zhanjiang experimental field of the South China Seed Breeding Center. After removing stones and plant roots, the soil samples were air-dried and passed through a 0.25 mm sieve for later use.

[0070] (2) Grouping: 2.1) blank control (only water added), 2.2) treatment with the conditioner prepared in Example 2 (0.5 g / kg soil), 2.3) treatment with the conditioner prepared in Example 3 (0.25 g / kg soil), 2.4) treatment with the conditioner prepared in Example 3 (0.5 g / kg soil), 2.5) treatment with the conditioner prepared in Example 3 (0.75 g / kg soil).

[0071] (3) Experimental method: prepare several culture pots, take 1 kg of the soil sample prepared in step (1) in each pot, and dilute the conditioners with clean water in proportion according to the treatment settings of (2) above, and add only clean water to the blank control. The amount of liquid added to each pot is controlled at 50 mL. Each treatment is repeated 3 times. The soil of each treatment is placed in a culture tank for closed culture for 30 days. During the entire experiment, deionized water is used to control the soil moisture at 70% of the field water holding capacity. On the 3rd, 5th, 10th, 15th, and 30th days after the start of the experiment, the gas at the top of the culture tank is collected, and the CO2 content in the gas is determined by titration, and the cumulative soil respiration (g C / kg dry soil) is calculated. The cumulative soil respiration is used to characterize the stability of soil organic matter. Destructive sampling was performed after 30 days: the wet sieving method was used to determine the content of super-large aggregates (>2 mm), large aggregates (1-2 mm) and small aggregates (0.25-1 mm); the soil samples were ultrasonically treated and passed through a 53 μm sieve, and the content of particulate organic matter components (>53 μm) and mineral-bound organic matter components (<53 μm) were determined by the H2SO4-K2Cr2O7 redox method, and each indicator was measured 5 times in parallel. The results are shown in Table 2.

[0072] Table 2

[0073]

[0074] It can be seen from the data results in Table 2 that the soil conditioner involved in the present invention has excellent effects of promoting soil aggregate formation and improving the stability of brick red soil organic matter. Comparing the results of the soil conditioner in Example 3 with different application amounts, it is found that the results of the 0.5g / kg treatment group and the 0.75g / kg treatment group are better than the results of the 0.25g / kg treatment group, and the results of the 0.5g / kg treatment group and the 0.75g / kg treatment group are close.

[0075] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0076] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0077] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A soil conditioner for improving the stability of organic matter in red soil, characterized in that: The soil conditioner comprises microalgae, humic acid or derivatives thereof; the microalgae comprises Scenedesmus and / or Chlorella.

2. The soil conditioner for improving the organic matter stability of lateosoil according to claim 1, characterized in that: The humic acid or its derivatives include any one of potassium humate, potassium nitrohumate or potassium fulvic acid or a combination of at least two thereof, preferably potassium humate.

3. The soil conditioner for improving the organic matter stability of lateosoil according to claim 1 or 2, characterized in that: The Scenedesmus sp. is a Scenedesmus sp. with a preservation number of GDMCC No. 63703, named as Scenedesmus sp. CJ, and is preserved in Guangdong Microbial Culture Collection Center.

4. The soil conditioner for improving the stability of organic matter in lateosol according to any one of claims 1 to 3, characterized in that: The chlorella is a chlorella with a preservation number of GDMCC No. 63704, which is named Chlorella sp. ZJ and is preserved in Guangdong Provincial Microbial Culture Collection Center.

5. The soil conditioner for improving the stability of organic matter in lateosol according to any one of claims 1 to 4, characterized in that: The microalgae are in the form of microalgae liquid or microalgae powder; Preferably, the amount of microalgae used is per gram of humic acid or its derivatives, the number of microalgae cells is (1×10 5 )-(1×10 7 )indivual.

6. The soil conditioner for improving the stability of organic matter in lateosol according to claim 5, characterized in that: The microalgae liquid is prepared by the following method: The microalgae are inoculated into a culture medium and cultured under light; Preferably, the culture temperature is 25-35°C, and the light intensity is 2000-12000 Lux; Preferably, the culture medium is BG-11 medium; Preferably, the culturing is performed until the microalgae cell concentration reaches (1×10 7 )-(5×10 7 ) / mL were harvested.

7. The soil conditioner for improving the stability of organic matter in lateosol according to claim 5, characterized in that: The mass ratio of the microalgae liquid to humic acid or its derivatives is (700-900):(30-100).

8. Use of the soil conditioner according to any one of claims 1 to 7 in improving the aggregate structure of red soil.

9. Use of the soil conditioner according to any one of claims 1 to 7 in improving the stability of organic matter in red soil.

10. The use according to claim 8 or 9, characterized in that: The method for using the soil conditioner is: dilute the soil conditioner with water and then spray it on the soil surface.

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