Sample culture device for pathogen diagnosis

Abstract

The invention discloses a sample culture device for pathogen diagnosis, and aims to solve the problems that when a current sample culture device for pathogen diagnosis is used for culturing a pathogen sample, the heating is not uniform, and the pathogen sample culture is not uniform. The sample culture device for pathogen diagnosis structurally comprises a culture box, a culture chamber and a culture tube treatment chamber are formed in the culture box, a separation plate is mounted in the culture chamber, and a plurality of culture tubes are arranged in the separation plate. A plurality of ultrasonic transducers are installed at the bottom of the isolation plate, temperature sensors are installed on the two sides of the isolation plate, and a heating plate is fixedly connected to the middle of the isolation plate. According to the pathogen sample culture device disclosed by the invention, the treated culture tube body is dried, so that the convenience of the culture device during treatment after culture of a pathogen sample is improved, and the labor intensity of an operator during treatment of the pathogen sample culture device is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of sample culture devices, in particular to a sample culture device for pathogen diagnosis. Background Art

[0002] Microorganisms such as bacteria, viruses, parasites, etc., can be life-threatening infectious diseases when detected in a patient. Often, the ability to save lives depends on the immediate identification of the pathogen so that appropriate antimicrobial therapy can be initiated. Certain infectious diseases can be detected and identified directly from analysis of biological specimens, while others require culturing the specimen to increase the number of microorganisms to detectable levels. As for the latter, incubators have proven to be invaluable tools. In short, the biological specimen is placed in an incubator and allowed to proliferate under growth-promoting conditions. After a certain period of time, the microorganisms (if present) become detectable.

[0003] In the process of realizing the invention, the inventors found that there are at least the following problems in the prior art that have not been solved: during use, the traditional pathogen diagnosis sample culture device is prone to uneven heating and uneven pathogen sample culture when culturing pathogen samples, and the culture tubes on the pathogen diagnosis sample culture device cannot be automatically cleaned and dried after use. Therefore, it is necessary to design a new technical solution to solve this problem. Summary of the invention

[0004] The purpose of the present invention is to provide a sample culture device for pathogen diagnosis to solve the technical problems that the current sample culture device for pathogen diagnosis is prone to uneven heating and uneven culture of pathogen samples when culturing pathogen samples, and the culture tubes on the sample culture device for pathogen diagnosis cannot be automatically cleaned and dried after use.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a sample culture device for pathogen diagnosis, comprising an incubator, wherein a culture chamber and a culture tube processing chamber are respectively provided inside the incubator, an isolation plate is installed inside the incubator, a plurality of ultrasonic transducers are installed at the bottom of the isolation plate, temperature sensors are installed on both sides of the isolation plate, a heating plate is fixedly connected to the middle of the isolation plate, a plurality of ultrasonic transducers are connected to an ultrasonic generator via wires, and limiting strips are fixedly connected to the four corners inside the incubator, and a culture tube processing chamber is installed at the top of the limiting strip. A culture tube placement rack is provided with a plurality of jacks, the interiors of the plurality of jacks are connected to culture tube bodies via rubber rings, a liquid guide tube is installed at the bottom of the culture tube processing chamber, one end of the liquid guide tube is sealedly connected to a nozzle, the other end of the liquid guide tube is connected to a pipe joint via a connecting pipe, the other end of the pipe joint is connected to an air outlet pipe and a liquid outlet pipe via a first control valve and a second control valve, one end of the air outlet pipe is connected to a hot air blower, the other end of the liquid outlet pipe is connected to a liquid storage tank via a liquid suction pump, and four limit blocks are installed on the four walls inside the culture tube processing chamber.

[0006] As a preferred embodiment of the present invention, two sealing cover plates are sealedly connected to the top of the incubator, and a controller is fixedly connected to the outer surface of the incubator.

[0007] As a preferred embodiment of the present invention, the detection end of the temperature sensor is located on the upper surface of the isolation plate, the four sides of the isolation plate are sealed to the inner wall of the culture chamber, and the ultrasonic generator is fixedly connected to one end outside the incubator.

[0008] As a preferred embodiment of the present invention, the outer ring of the rubber ring is fixedly connected to the inner wall of the insertion hole, and the outer part of the culture tube body is plugged into the inner ring of the rubber ring.

[0009] As a preferred embodiment of the present invention, one end of the liquid guide tube is sealed connected to one end of the connecting tube, and the other end of the connecting tube is sealed connected to one end of the pipe joint, and the socket position of the nozzle on the culture tube placement rack corresponds.

[0010] As a preferred embodiment of the present invention, one end of the first control valve is sealed and connected to one side of the pipe joint, and the other end of the first control valve is sealed and connected to one end of the air outlet pipe.

[0011] As a preferred embodiment of the present invention, the other end of the air outlet pipe is sealed and connected to one end of the hot air blower, and the other end of the hot air blower is fixedly connected to one side of the liquid storage tank.

[0012] As a preferred embodiment of the present invention, one end of the second control valve is sealed connected to the other end of the pipeline joint, and the other end of the second control valve is sealed connected to one end of the liquid outlet pipe.

[0013] As a preferred embodiment of the present invention, the other end of the liquid outlet pipe is sealed connected to one end of the liquid pump, the other end of the liquid pump is sealed connected to one end of the liquid storage tank, and the liquid storage tank is fixedly connected to the outside of the incubator.

[0014] As a preferred embodiment of the present invention, the bottom end of the limit block is located above the nozzle and is fixedly connected to the four walls inside the culture tube processing chamber.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] When the pathogen sample is cultured, the pathogen sample is first placed inside the culture tube body, and then the top of the culture tube body is sealed and inserted into the jack of the culture tube placement rack. At this time, the rubber ring will generate a certain squeezing force and friction force on the culture tube body, thereby preventing the culture tube body from shaking or automatically falling off from the culture tube placement rack, thereby improving the stability of the pathogen sample during culture and cleaning.

[0017] After the culture tube is installed, the culture tube rack is placed on the limit bar, and then the sealing cover is covered, and then the ultrasonic generator generates ultrasonic waves to the water in the culture chamber through the ultrasonic transducer, so that the water temperature in the culture chamber can be evenly mixed, and the pathogen sample in the culture tube body can be shaken evenly. At the same time, the heating plate will heat the water to heat the pathogen sample. When the water temperature reaches the detection range of the temperature sensor, the controller will control the heating plate to stop heating, so as to prevent the pathogen sample from being scalded due to excessive water temperature;

[0018] When the culture tube body needs to be cleaned, the culture tube rack is directly taken out of the culture chamber, and after the culture tube body is inverted, the culture tube rack is placed on the limit block in the culture tube processing chamber, and then the second control valve is opened, the first control valve is closed, and the liquid pump is used to extract the disinfectant in the liquid storage tank through the liquid outlet pipe, and transport it to the liquid guide pipe through the connecting pipe, and then sprayed out by the nozzle. At this time, the disinfectant will rinse the inside of the culture tube body. After the culture tube body is processed, the second control valve is closed and the first control valve is opened. At this time, the hot air machine generates hot air, and then the air outlet pipe transports it to the liquid guide pipe through the connecting pipe, and then the hot air is sprayed out by the nozzle, so as to achieve the drying of the processed culture tube body, thereby improving the convenience of the culture device for post-culture processing of pathogen samples and reducing the labor intensity of the operator when processing the pathogen sample culture device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0020] Figure 1 It is a structural schematic diagram of the present invention;

[0021] Figure 2 It is a schematic diagram of the structure of the liquid storage tank of the present invention;

[0022] Figure 3 is a cross-sectional view of the present invention;

[0023] Figure 4 A top view of the present invention;

[0024] In the figure: 1. incubator; 11. sealing cover; 12. controller;

[0025] 2. Cultivation chamber; 21. Isolation plate; 22. Ultrasonic transducer; 23. Temperature sensor; 24. Heating plate; 25. Ultrasonic generator; 26. Limiting strip;

[0026] 3. Culture tube processing room; 31. Liquid guide tube; 32. Nozzle; 33. Connecting pipe; 34. Pipe joint; 35. First control valve; 36. Second control valve; 37. Air outlet pipe; 38. Liquid outlet pipe; 39. Hot air blower;

[0027] 40. Liquid pump; 41. Liquid storage tank; 42. Limit block;

[0028] 5. Culture tube placement rack; 51. Socket; 52. Rubber ring; 53. Culture tube body. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0030] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0031] Example 1: A sample culture device for pathogen diagnosis, see Figures 1 to 4, comprising an incubator 1, wherein a culture chamber 2 and a culture tube processing chamber 3 are respectively provided inside the incubator 1, an isolation plate 21 is installed inside the incubator 2, a plurality of ultrasonic transducers 22 are installed on the bottom of the isolation plate 21, temperature sensors 23 are installed on both sides of the isolation plate 21, a heating plate 24 is fixedly connected to the middle of the isolation plate 21, a plurality of ultrasonic transducers 22 are connected to an ultrasonic generator 25 through a wire, a detection end of the temperature sensor 23 is located on the upper surface of the isolation plate 21, the surrounding of the isolation plate 21 is sealed with the inner wall of the culture chamber 2, the ultrasonic generator 25 is fixedly connected to one end outside the incubator 1, and the temperature sensor 23 is connected to the inner wall of the culture chamber 2. After the culture tube is installed, the culture tube rack 5 is placed on the limit strip 26, and then the sealing cover plate 11 is covered, and then the ultrasonic generator 25 generates ultrasonic waves to the water in the culture chamber 2 through the ultrasonic transducer 22, so that not only the water temperature in the culture chamber 2 can be mixed evenly, but also the pathogen sample in the culture tube body 53 can be shaken evenly. At the same time, the heating plate 24 will heat the water to heat the pathogen sample. When the water temperature reaches the detection range of the temperature sensor 23, the controller 12 will control the heating plate 24 to stop heating, so as to prevent the pathogen sample from being scalded by excessive water temperature;

[0032] The four corners inside the culture chamber 2 are fixedly connected to the limiting strips 26, and a culture tube rack 5 is installed at the top of the limiting strips 26. The culture tube rack 5 is provided with a plurality of plug holes 51, and the interior of the plurality of plug holes 51 is connected to a culture tube body 53 through a rubber ring 52. The outer ring of the rubber ring 52 is fixedly connected to the inner wall of the plug hole 51, and the exterior of the culture tube body 53 is plugged into the inner ring of the rubber ring 52. When the pathogen sample is cultured, the pathogen sample is first placed inside the culture tube body 53, and then the top of the culture tube body 53 is sealed and inserted into the plug hole 51 of the culture tube rack 5. At this time, the rubber ring 52 will generate a certain squeezing force and friction force on the culture tube body 53, thereby preventing the culture tube body 53 from shaking or automatically falling off from the culture tube rack 5, thereby improving the stability of the pathogen sample during culture and cleaning.

[0033] A liquid guide tube 31 is installed at the bottom of the culture tube processing chamber 3, one end of the liquid guide tube 31 is sealed and connected to a nozzle 32, the other end of the liquid guide tube 31 is connected to a pipe joint 34 through a connecting pipe 33, the other end of the pipe joint 34 is connected to an air outlet pipe 37 and a liquid outlet pipe 38 through a first control valve 35 and a second control valve 36, one end of the air outlet pipe 37 is connected to a hot air blower 39, the other end of the liquid outlet pipe 38 is connected to a liquid storage tank 41 through a liquid pump 40, four limit blocks 42 are installed on the four walls inside the culture tube processing chamber 3, one end of the liquid guide tube 31 is sealed and connected to one end of the connecting pipe 33, and the other end of the connecting pipe 33 is sealed and connected to At one end of the pipe joint 34, the position of the socket 51 of the nozzle 32 on the culture tube placement rack 5 corresponds, one end of the first control valve 35 is sealed and connected to one side of the pipe joint 34, the other end of the first control valve 35 is sealed and connected to one end of the air outlet pipe 37, the other end of the air outlet pipe 37 is sealed and connected to one end of the hot air blower 39, the other end of the hot air blower 39 is fixedly connected to one side of the liquid storage tank 41, one end of the second control valve 36 is sealed and connected to the other end of the pipe joint 34, the other end of the second control valve 36 is sealed and connected to one end of the liquid outlet pipe 38, the other end of the liquid outlet pipe 38 is sealed and connected to one end of the liquid pump 40, The other end of the liquid pump 40 is sealed and connected to one end of the liquid storage tank 41, and the liquid storage tank 41 is fixedly connected to the outside of the incubator 1. The bottom end of the limit block 42 is located above the nozzle 32 and is fixedly connected to the four walls inside the culture tube processing chamber 3. When the culture tube body 53 needs to be cleaned, the culture tube placement rack 5 is directly taken out from the culture chamber 2, and after the culture tube body 53 is inverted, the culture tube placement rack 5 is placed on the limit block 42 in the culture tube processing chamber 3, and then the second control valve 36 is opened, the first control valve 35 is closed, and then the liquid pump 40 is used to extract the disinfectant in the liquid storage tank 41 through the liquid outlet pipe 38, and then the disinfectant is removed from the liquid storage tank 41. The disinfectant is then transported to the liquid guide tube 31 through the connecting tube 33 and then sprayed out by the nozzle 32. At this time, the disinfectant will rinse the inside of the culture tube body 53. After the culture tube body 53 is treated, the second control valve 36 is closed and the first control valve 35 is opened. At this time, the hot air blower 39 will generate hot air, and the air outlet pipe 37 transports the hot air to the liquid guide tube 31 through the connecting tube 33, and then the hot air is sprayed out by the nozzle 32, so that the treated culture tube body 53 is dried, thereby improving the convenience of the culture device for post-culture treatment of pathogen samples and reducing the labor intensity of the operator when handling the pathogen sample culture device.

[0034] For details, see Figure 1 The top of the incubator 1 is sealed with two sealing cover plates 11 , and the outer surface of the incubator 1 is fixedly connected with a controller 12 .

[0035] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0036] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A sample culture device for pathogen diagnosis, comprising an incubator (1), characterized in that: The culture box (1) is provided with a culture chamber (2) and a culture tube processing chamber (3) respectively inside. An isolation plate (21) is installed inside the culture chamber (2). A plurality of ultrasonic transducers (22) are installed on the bottom of the isolation plate (21). Temperature sensors (23) are installed on both sides of the isolation plate (21). A heating plate (24) is fixedly connected to the middle of the isolation plate (21). The plurality of ultrasonic transducers (22) are connected to an ultrasonic generator (25) via wires. Limiting strips (26) are fixedly connected to the four corners inside the culture chamber (2). A culture tube placing rack (5) is installed on the top of the limiting strip (26). The culture tube placing rack (5) is provided with a plurality of jacks (51). The plurality of jacks (52) are connected to the culture tube placing rack (5). The interior of the culture tube treatment chamber (3) is connected to a culture tube body (53) via a rubber ring (52); a liquid guide tube (31) is installed at the bottom of the culture tube treatment chamber (3); one end of the liquid guide tube (31) is sealedly connected to a nozzle (32); the other end of the liquid guide tube (31) is connected to a pipe joint (34) via a connecting pipe (33); the other end of the pipe joint (34) is connected to an air outlet pipe (37) and a liquid outlet pipe (38) via a first control valve (35) and a second control valve (36); one end of the air outlet pipe (37) is connected to a hot air blower (39); the other end of the liquid outlet pipe (38) is connected to a liquid storage tank (41) via a liquid pump (40); and four limit blocks (42) are installed on the four walls inside the culture tube treatment chamber (3).

2. A sample culture device for pathogen diagnosis according to claim 1, characterized in that: Two sealing cover plates (11) are sealedly connected to the top of the incubator (1), and a controller (12) is fixedly connected to the outer surface of the incubator (1).

3. A sample culture device for pathogen diagnosis according to claim 1, characterized in that: The detection end of the temperature sensor (23) is located on the upper surface of the isolation plate (21), the four sides of the isolation plate (21) are sealed and connected to the inner wall of the culture chamber (2), and the ultrasonic generator (25) is fixedly connected to one end outside the culture box (1).

4. A sample culture device for pathogen diagnosis according to claim 1, characterized in that: The outer ring of the rubber ring (52) is fixedly connected to the inner wall of the insertion hole (51), and the outside of the culture tube body (53) is plugged into the inner ring of the rubber ring (52).

5. The sample culture device for pathogen diagnosis according to claim 1, characterized in that: One end of the liquid guide tube (31) is sealedly connected to one end of the connecting tube (33), and the other end of the connecting tube (33) is sealedly connected to one end of the pipe joint (34), and the position of the socket (51) of the nozzle (32) located on the culture tube placement rack (5) corresponds.

6. A sample culture device for pathogen diagnosis according to claim 1, characterized in that: One end of the first control valve (35) is sealed and connected to one side of the pipe joint (34), and the other end of the first control valve (35) is sealed and connected to one end of the air outlet pipe (37).

7. The sample culture device for pathogen diagnosis according to claim 1, characterized in that: The other end of the air outlet pipe (37) is sealedly connected to one end of the hot air blower (39), and the other end of the hot air blower (39) is fixedly connected to one side of the liquid storage tank (41).

8. The sample culture device for pathogen diagnosis according to claim 1, characterized in that: One end of the second control valve (36) is sealed and connected to the other end of the pipeline joint (34), and the other end of the second control valve (36) is sealed and connected to one end of the liquid outlet pipe (38).

9. The sample culture device for pathogen diagnosis according to claim 1, characterized in that: The other end of the liquid outlet pipe (38) is sealedly connected to one end of the liquid pump (40), and the other end of the liquid pump (40) is sealedly connected to one end of the liquid storage tank (41), and the liquid storage tank (41) is fixedly connected to the outside of the incubator (1).

10. The sample culture device for pathogen diagnosis according to claim 1, characterized in that: The bottom end of the limiting block (42) is located above the nozzle (32) and is fixedly connected to the four walls inside the culture tube processing chamber (3).

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