Sample pretreatment system and treatment method

Abstract

The invention discloses a sample pretreatment system and method, and belongs to the field of sample pretreatment, the sample pretreatment system comprises a transfer module, and a sample bin, a solid phase extraction module, an oscillation module, a centrifugation module, a parallel concentration module and a nitrogen blowing concentration module distributed around the transfer module, the transfer module, the sample bin, the solid-phase extraction module, the oscillation module, the centrifugation module, the parallel concentration module and the nitrogen blowing concentration module are independently arranged. According to the invention, the whole pretreatment is split into module combinations, so that the space limitation of the equipment is relieved, and the equipment can be suitable for being arranged and placed in various laboratories; operation logic for freely editing an experiment process can be established, and the problem of equipment operation efficiency is solved; all the modules can be independent and can perform processing actions simultaneously; the degree of freedom is high, efficiency is high, and unmanned operation can be achieved.

Description

Technical Field

[0001] The invention belongs to the field of sample pre-processing, and in particular relates to a sample pre-processing system and a processing method. Background Art

[0002] Sample pretreatment, also known as sample pretreatment, is a crucial step in the sample detection and analysis process. It involves processes such as sample decomposition, extraction, purification and concentration, aiming to convert the sample into a form suitable for analysis.

[0003] As laboratories develop, small laboratories are closed or merged, and commercial laboratories will definitely develop in the direction of large-scale and comprehensive. At the same time, as society pays more attention to the health field (food safety, environmental monitoring, life sciences), it will strengthen investment and monitoring in this area, so the number of samples will also increase. However, in current laboratories, the stability of personnel has always been the biggest factor affecting the scale of laboratories.

[0004] Therefore, the demand for automation has always been the focus of the laboratory. The current laboratory automation can only solve a single process or a few combined processes, which can reduce the laboratory's dependence on inspectors. However, its several combined processes cannot run independently, cannot perform processing actions at the same time, and are affected by space limitations, and the operating efficiency needs to be improved. Summary of the invention

[0005] The purpose of the present invention is to provide a sample pre-processing system and a processing method to overcome at least one of the above-mentioned defects in the prior art.

[0006] To achieve this object, the present invention adopts the following technical solutions: The present invention provides a sample pretreatment system, comprising a transport module, and a sample bin, a solid phase extraction module, an oscillation module, a centrifugal module, a parallel concentration module, and a nitrogen blowing concentration module distributed around the transport module. The transport module, the sample bin, the solid phase extraction module, the oscillation module, the centrifugal module, the parallel concentration module, and the nitrogen blowing concentration module are independently arranged. The oscillation module has a mixing device, and the mixing device includes a bottom plate, a first guide column, a first guide sleeve, a first spring, a first mounting frame, a pusher, a transmission member, a stirring member, a linear module, and a second mounting frame. A first guide column is fixed on both sides of the left and right sides of the top wall of the base plate, a first guide sleeve is fixed on both sides of the left and right sides of the top wall of the first mounting frame, the first guide column passes through the top wall of the first mounting frame and the first guide sleeve, a first spring is fixed between the base plate and the first mounting frame, the first spring is arranged around the first guide column, the linear module is fixed to the top wall of the base plate, the sliding end of the linear module is fixed to the second mounting frame, the pushing member is fixed to the lower part of the second mounting frame, the stirring member is fixed to the upper part of the second mounting frame, the pushing member is connected to the stirring member through a transmission member, and the pushing member is used to push the first mounting frame to move downward.

[0007] Preferably, the first mounting frame includes a first horizontal plate, a vertical rod, and a second horizontal plate. Vertical rods are fixed on both sides of the left and right sides of the bottom wall of the first horizontal plate, the second horizontal plate is fixed at the bottom end of the vertical rod, the pushing member is located above the second horizontal plate, and the first guide sleeve is fixed to the top wall of the first horizontal plate.

[0008] Preferably, the pushing member includes a motor, a first rotating shaft, and a cam. The first rotating shaft is fixed to the front end of the motor. The cam is fixed to the front end of the first rotating shaft. The cam is located above the second transverse plate.

[0009] Preferably, the second mounting frame includes a third horizontal plate and a vertical plate. The third horizontal plate is fixed to the bottom end of the sliding end of the linear module, and the vertical plate is fixed to the bottom wall of the third horizontal plate. Agitators are fixed on both sides of the bottom wall of the third horizontal plate, and the motor is fixed to the front side wall of the lower part of the vertical plate.

[0010] Preferably, the stirring member includes a third mounting frame, a first bearing seat, a stirring shaft, and a stirring blade. The third mounting frame is fixed to the bottom wall of the third horizontal plate, the first bearing seat is fixed to the middle part of the inner bottom wall of the third mounting frame, the bottom end of the stirring shaft passes through the first bearing seat and the bottom wall of the third mounting frame, and extends to the bottom of the third mounting frame where a stirring blade is fixed.

[0011] Preferably, it also includes a second guide sleeve, a second guide column, a second spring, and a baffle. The second guide sleeve is fixed on both sides of the left and right sides of the inner bottom wall of the third mounting frame. The bottom end of the second guide column passes through the second guide sleeve and the bottom wall of the third mounting frame and is fixed with a baffle. A second spring is fixed between the baffle and the third mounting frame. The second spring is arranged around the second guide column. The stirring shaft passes through the baffle, and the stirring blade is located below the baffle.

[0012] The transmission member includes a first transmission wheel, a first transmission belt, a second transmission wheel, a second rotating shaft, a second bearing seat, a first bevel gear, a second bevel gear, a third rotating shaft, a third bearing seat, a double-groove transmission wheel, a third transmission wheel, and a second transmission belt, the first rotating shaft is fixed with the first transmission wheel, the first transmission wheel is located on the rear side of the cam, the front side wall of the upper part of the vertical plate is fixed with the second bearing seat, the rear end of the second rotating shaft is inserted into the second bearing seat, the second transmission wheel and the first bevel gear are both fixed to the second rotating shaft, the second transmission wheel is located on the front side of the first bevel gear, the second transmission wheel is connected to the first transmission wheel through the first transmission belt, the bottom wall of the third horizontal plate is fixed with the third bearing seat, the top end of the third rotating shaft is inserted into the third bearing seat, the second bevel gear is fixed to the bottom end of the third rotating shaft, the second bevel gear is meshed with the first bevel gear, the middle part of the third rotating shaft is fixed with a double-groove transmission wheel, the upper part of each agitator shaft is fixed with the third transmission wheel, and the third transmission wheel and the double-groove transmission wheel are connected through the second transmission belt.

[0013] Preferably, the sample chamber, solid phase extraction module, oscillation module, centrifugation module, parallel concentration module, and nitrogen blowing concentration module all have positioning pins, and the positioning pins of the mixing device are arranged on the left and right sides of the top wall of the first horizontal plate.

[0014] Preferably, a sample rack is further included, and the bottom wall of the sample rack has a positioning hole that cooperates with the positioning pin.

[0015] The present invention also provides a sample pretreatment method, which adopts the above-mentioned sample pretreatment system for treatment, and comprises the following steps: performing initial sampling in a sample bin, after the initial sampling is completed, transporting the sample rack to an oscillation module for mixed oscillation treatment through a transfer module, after the mixed oscillation treatment is completed, transporting the sample rack to a centrifugal module for centrifugal treatment through a transfer module, after the centrifugal treatment is completed, transporting the sample rack to a nitrogen blowing concentration module for nitrogen blowing concentration treatment through a transfer module, after the nitrogen blowing concentration treatment is completed, transporting the sample rack to a parallel concentration module for parallel concentration treatment through a transfer module, after the parallel concentration treatment is completed, transporting the sample rack to a solid phase extraction module for solid phase extraction treatment through a transfer module, after the solid phase extraction treatment is completed, transporting the sample rack back to the sample bin through a transfer module, and completing the sample pretreatment.

[0016] The beneficial effects of the present invention are: 1. Through each independently set module, each module is arranged around the transfer module, and the sample rack is transferred between modules through the transfer module without manual processing; by splitting the entire pre-treatment into a module combination, the space limitation of the equipment is removed, and the equipment can be adapted to various laboratory layouts; the operation logic of the experimental process can be freely edited to solve the problem of equipment operation efficiency; each module can be independent and can perform processing actions at the same time; it not only has a high degree of freedom, but also is efficient and can realize unmanned operation.

[0017] 2. While stirring and mixing, the relative position of the sample tube and the stirring element is changed, that is, the stirring position is changed, which effectively breaks the sample laminar flow phenomenon, makes the mixing more complete and uniform, helps to accelerate the mixing, improve the mixing efficiency, and makes the sample form a more complex flow pattern in the sample tube, further improving the mixing effect.

[0018] 3. While stirring and mixing, the sample tube is driven to move up and down to form a three-dimensional mixture, which greatly improves the mixing uniformity, reduces the mixing dead angle, accelerates the mixing process, and improves the mixing efficiency.

[0019] 4. Through the setting of the transmission member, only one driving source is needed to realize the pushing operation of the pushing member and the stirring operation of the stirring member. The design is ingenious and reduces costs.

[0020] 5. The use of nonlinear shaking can break the fixed flow pattern produced by linear shaking, allowing the sample in the sample tube to move more complexly in three-dimensional space, which helps to reduce mixing dead angles and allows the sample to be fully mixed in all directions and levels; the shear force and impact force generated by nonlinear shaking are usually stronger than linear shaking, which helps to accelerate the sample mixing process; nonlinear shaking has higher flexibility and adaptability. It can achieve the best mixing effect by adjusting the frequency, amplitude and other parameters of shaking according to the characteristics and mixing requirements of different samples. Nonlinear shaking can achieve full mixing of samples in a shorter time, thereby shortening the mixing time. Nonlinear shaking can also reduce energy consumption and improve stirring efficiency by optimizing shaking parameters. Nonlinear effects may help break the interfacial tension between materials and promote interaction and exchange between materials.

[0021] 6. The driving member and the stirring member are linked by the transmission member. The specific structural design of the transmission member changes the rotation axis, and can simultaneously drive the stirring members on the left and right sides to perform synchronous rotation and stirring operations, thereby improving the mixing efficiency.

[0022] 7. The baffle is set to block the opening of the sample tube to prevent the sample from spilling out during the stirring, mixing and up and down shaking process. The second spring is set to ensure that the baffle always blocks the opening of the sample tube during the up and down movement of the sample tube.

[0023] 8. The positioning pins and the positioning holes can be arranged to realize the rapid positioning of the sample rack, realize the rapid docking of the sample rack in each module, and ensure the stability of the sample rack after placement, especially in the mixing device. The positioning pins and the positioning holes can be arranged to prevent the sample rack from being separated from the first mounting rack during the up and down reciprocating motion of the first mounting rack.

[0024] 9. The second spring and the baffle can also ensure that the sample tube and the sample rack will not separate during the up and down reciprocating movement of the first mounting frame. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a three-dimensional structural schematic diagram of the present invention.

[0026] Figure 2 It is a schematic diagram of the top structure of the present invention.

[0027] Figure 3 It is a schematic diagram of the main structure of the mixing device of the present invention (first state).

[0028] Figure 4 It is a schematic diagram of the main structure of the first mounting frame, the first guide sleeve, the first spring, the base plate, and the positioning pin of the present invention.

[0029] Figure 5It is a schematic diagram of the structure of the sample rack of the present invention when viewed from above.

[0030] Figure 6 It is a schematic diagram of the front view structure of the second mounting frame, the pushing member, and part of the transmission member of the present invention.

[0031] Figure 7 It is a schematic diagram of the main structure of the stirring member, the second guide sleeve, the second guide column, the second spring, and the baffle of the present invention.

[0032] Figure 8 It is a partial front structural schematic diagram of the transmission part of the present invention.

[0033] Fig. 9 It is a schematic diagram of the main structure of the mixing device of the present invention (second state).

[0034] The markings in the accompanying drawings are: 100-transport module, 200-sample chamber, 300-solid phase extraction module, 400-oscillation module, 500-centrifugation module, 600-parallel concentration module, 700-nitrogen blowing concentration module, 401-mixing device, 1-bottom plate, 2-first guide column, 3-first guide sleeve, 4-first spring, 5-first mounting frame, 6-pushing member, 7-transmission member, 8-stirring member, 9-second mounting frame, 10-linear module, 51-first horizontal plate, 52-vertical rod, 53-second horizontal plate, 61-motor, 62-first rotating shaft, 63-cam, 91-third Horizontal plate, 92-vertical plate, 81-third mounting frame, 82-first bearing seat, 83-stirring shaft, 84-stirring blade, 11-second guide sleeve, 12-second guide column, 13-second spring, 14-baffle, 71-first transmission wheel, 72-first transmission belt, 73-second transmission wheel, 74-second rotating shaft, 75-second bearing seat, 76-first bevel gear, 77-second bevel gear, 78-third rotating shaft, 79-third bearing seat, 710-double-groove transmission wheel, 711-third transmission wheel, 712-second transmission belt, 15-positioning pin, 16-sample rack, 17-positioning hole. DETAILED DESCRIPTION

[0035] The present invention will now be further described with reference to the accompanying drawings and specific implementation methods.

[0036] Contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field. In the description of the present invention, it should be understood that the orientation or position relationship indicated by the terms "center", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. are based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0037] like Figures 1 to 9 As shown, a sample pretreatment system provided in this embodiment includes a transport module 100, and a sample bin 200, a solid phase extraction module 300, an oscillation module 400, a centrifugal module 500, a parallel concentration module 600, and a nitrogen blowing concentration module 700 distributed around the transport module 100. The transport module 100, the sample bin 200, the solid phase extraction module 300, the oscillation module 400, the centrifugal module 500, the parallel concentration module 600, and the nitrogen blowing concentration module 700 are independently arranged, and the oscillation module 400 has a mixing device 401, and the mixing device 401 includes a bottom plate 1, a first guide column 2, a first guide sleeve 3, a first spring 4, a first mounting frame 5, a pusher 6, and a transmission member 7. , a stirring member 8, a linear module 10, and a second mounting frame 9. The first guide columns 2 are fixed on the left and right sides of the top wall of the base plate 1, the first guide sleeves 3 are fixed on the left and right sides of the top wall of the first mounting frame 5, the first guide column 2 passes through the top wall of the first mounting frame 5 and the first guide sleeve 3, a first spring 4 is fixed between the base plate 1 and the first mounting frame 5, and the first spring 4 is arranged around the first guide column 2. The linear module 10 is fixed to the top wall of the base plate 1, and the second mounting frame 9 is fixed to the sliding end of the linear module 10. The pushing member 6 is fixed to the lower part of the second mounting frame 9, the stirring member 8 is fixed to the upper part of the second mounting frame 9, the pushing member 6 is connected to the stirring member 8 through the transmission member 7, and the pushing member 6 is used to push the first mounting frame 5 to move downward.

[0038] This embodiment also provides a sample pretreatment method, which uses the above-mentioned sample pretreatment system for processing, including the following steps: Initial sampling is performed in the sample bin 200. After the initial sampling is completed, the sample rack 16 is transported to the oscillation module 400 for mixed oscillation treatment through the transfer module 100. After the mixed oscillation treatment is completed, the sample rack 16 is transported to the centrifugation module 500 for centrifugation treatment through the transfer module 100. After the centrifugation treatment is completed, the sample rack 16 is transported to the nitrogen blowing concentration module 700 for nitrogen blowing concentration treatment through the transfer module 100. After the nitrogen blowing concentration treatment is completed, the sample rack 16 is transported to the parallel concentration module 600 for parallel concentration treatment through the transfer module 100. After the parallel concentration treatment is completed, the sample rack 16 is transported to the solid phase extraction module 300 for solid phase extraction treatment through the transfer module 100. After the solid phase extraction treatment is completed, the sample rack 16 is transported back to the sample bin 200 through the transfer module 100 to complete the sample pre-treatment.

[0039] Through each independently set module, each module is arranged around the transfer module 100, and the sample rack 16 is transferred between each module through the transfer module 100 without manual processing. By splitting the entire pre-treatment into a module combination, the space limitation of the equipment is removed, and the equipment can be placed in various laboratory layouts; the operation logic of the free editable experimental process can be established to solve the problem of equipment operation efficiency; each module can be independent and can perform processing actions at the same time. Not only is the degree of freedom high, but it is also efficient and can realize unmanned operation.

[0040] In the oscillation module 400, in addition to the basic oscillation mixing process, the present invention also has a separate mixing device 401, which greatly improves the mixing efficiency. Specifically, the sample is stirred and mixed by the stirring member 8. While stirring and mixing, the first mounting frame 5 is intermittently pushed downward by the pushing member 6. Combined with the setting of the first spring 4, the first mounting frame 5 reciprocates up and down, driving the sample rack 16 to reciprocate up and down, and then driving the sample tube to reciprocate up and down, while the stirring position of the stirring member 8 remains unchanged, thereby changing the relative position of the sample tube and the stirring member 8, that is, changing the stirring position, effectively breaking the sample laminar flow phenomenon, mixing more fully and evenly, helping to accelerate mixing, improve mixing efficiency, and allowing the sample to form a more complex flow pattern in the sample tube, further improving the mixing effect. In addition to changing the relative position of the sample tube and the stirring member 8, the up and down reciprocating motion of the first mounting frame 5 also drives the sample tube to reciprocate up and down to form a three-dimensional mixing, greatly improving the mixing uniformity, reducing the mixing dead angle, accelerating the mixing process, and improving the mixing efficiency. During the mixing process, the sample rack 16 is grabbed by the manipulator of the oscillating module 400 and placed on the first mounting rack 5. The linear module 10 drives the second mounting rack 9 and its upper structure to move downward, so that the stirring end of the stirring member 8 extends into the sample tube, and at the same time drives the pushing member 6 to move downward. The pushing member 6 works to push the first mounting rack 5 downward, and drives the sample rack 16 to move downward. Fig. 9As shown, the first spring 4 contracts. When the pusher removes the force pushing the first mounting frame 5 downward, the first spring 4 stretches and resets, driving the first mounting frame 5 to move upward and reset, so that the sample rack 16 moves upward and resets. In this way, the sample tube can be driven to reciprocate up and down. The first guide sleeve 3 and the first guide column 2 cooperate to ensure the smooth up and down movement of the first mounting frame 5. Through the setting of the transmission member 7, only one driving source is needed to realize the pushing operation of the pusher 6 and the stirring operation of the stirring member 8. The design is ingenious and reduces costs.

[0041] The first mounting frame 5 includes a first transverse plate 51, a vertical rod 52, and a second transverse plate 53. The vertical rods 52 are fixed to the left and right sides of the bottom wall of the first transverse plate 51. The second transverse plate 53 is fixed to the bottom end of the vertical rod 52. The pusher 6 is located above the second transverse plate 53. The first guide sleeve 3 is fixed to the top wall of the first transverse plate 51. The pusher 6 includes a motor 61, a first rotating shaft 62, and a cam 63. The front end of the motor 61 is fixed with the first rotating shaft 62. The front end of the first rotating shaft 62 is fixed with the cam 63. The cam 63 is located above the second transverse plate 53. During the pushing operation, the motor 61 rotates, driving the first rotating shaft 62 to rotate, so that the cam 63 rotates. When the distal end of the cam 63 contacts the second transverse plate 53, as the motor 61 continues to rotate, the cam 63 pushes the second transverse plate 53 to move downward, so that the vertical rod 52 moves downward, driving the first transverse plate 51 to move downward, and then the sample rack 16 moves downward. When the distal end of the cam 63 leaves the second transverse plate 53, the first spring 4 drives the first transverse plate 51 to move upward, so that the vertical rod 52 moves upward, and drives the second transverse plate 53 to move upward. In this way, the motor 61 rotates continuously in one direction to realize the up-and-down reciprocating motion of the first mounting frame 5 and change the stirring position. Since the process of the first spring 4 driving the first mounting frame 5 to move upward and reset is nonlinear, the process of the cam 63 pushing the first mounting frame 5 to move downward is also nonlinear, that is, the nonlinear shaking of the sample tube is realized, which can break the fixed flow pattern generated by the linear shaking, so that the sample in the sample tube can move more complexly in three-dimensional space. This complex movement pattern helps to reduce the mixing dead angle, so that the sample can be fully mixed in all directions and levels; the shear force and impact force generated by nonlinear shaking are usually stronger than those of linear shaking, and this strong stirring effect helps to accelerate the sample mixing process; nonlinear shaking has higher flexibility and adaptability, and it can achieve the best mixing effect by adjusting the frequency, amplitude and other parameters of shaking according to the characteristics and mixing requirements of different samples, which is specifically changed by changing the speed of the motor 61. Nonlinear shaking can achieve full mixing of samples in a shorter time, thus shortening the mixing time. This is especially important for samples that need to be mixed quickly. In addition, nonlinear shaking can also reduce energy consumption and improve stirring efficiency by optimizing shaking parameters. The nonlinear effect may help break the interfacial tension between materials and promote interaction and exchange between materials. This effect is particularly important when processing certain special samples.

[0042] The second mounting frame 9 includes a third horizontal plate 91 and a vertical plate 92. The third horizontal plate 91 is fixed to the bottom end of the sliding end of the linear module 10. The vertical plate 92 is fixed to the bottom wall of the third horizontal plate 91. The stirring member 8 is fixed to the left and right sides of the bottom wall of the third horizontal plate 91. The motor 61 is fixed to the front side wall of the lower part of the vertical plate 92. The stirring member 8 includes a third mounting frame 81, a first bearing seat 82, a stirring shaft 83, and a stirring blade 84. The third mounting frame 81 is fixed to the bottom wall of the third horizontal plate 91. The first bearing seat 82 is fixed to the middle of the inner bottom wall of the third mounting frame 81. The bottom end of the stirring shaft 83 passes through the first bearing seat 82 and the bottom wall of the third mounting frame 81, and extends to the bottom of the third mounting frame 81 where the stirring blade 84 is fixed. The transmission member 7 includes a first transmission wheel 71, a first transmission belt 72, a second transmission wheel 73, a second rotating shaft 74, a second bearing seat 75, a first bevel gear 76, a second bevel gear 77, a third rotating shaft 78, a third bearing seat 79, a double-groove transmission wheel 710, a third transmission wheel 711, and a second transmission belt 712. The first rotating shaft 62 is fixed with the first transmission wheel, and the first transmission wheel is located at the rear side of the cam 63. The front side wall of the upper part of the vertical plate 92 is fixed with the second bearing seat 75. The rear end of the second rotating shaft 74 is inserted into the second bearing seat 75. The second transmission wheel 73 and the first bevel gear 76 are both fixed to the second rotating shaft 74. The second transmission wheel 73 is located at the front side of the first bevel gear 76, and the second transmission wheel 73 is connected to the first transmission wheel 71 through the first transmission belt 72. A third bearing seat 79 is fixed to the bottom wall of the third cross plate 91, and the top of the third rotating shaft 78 is inserted into the third bearing seat 79. The bottom end of the third rotating shaft 78 is fixed with a second bevel gear 77, and the second bevel gear 77 is meshed with the first bevel gear 76. A double-groove transmission wheel 710 is fixed to the middle of the third rotating shaft 78, and a third transmission wheel 711 is fixed to the upper part of each stirring shaft 83. The third transmission wheel 711 and the double-groove transmission wheel 710 are connected through a second transmission belt 712.

[0043] During the stirring and mixing operation, the stirring shaft 83 rotates, driving the stirring blade 84 to rotate for stirring and mixing. Specifically, the motor 61 rotates, driving the first rotating shaft 62 to rotate, so that the first transmission belt 72 rotates, and the second transmission belt 712 rotates through the first transmission belt 72, so that the second rotating shaft 74 rotates, driving the first bevel gear 76 to rotate, and then driving the second bevel gear 77 to rotate, so that the third rotating shaft 78 rotates, driving the double-groove transmission wheel 710 to rotate, and driving the third transmission wheels 711 on the left and right sides to rotate through the second transmission belt 712, so that the stirring shafts 83 on the left and right sides rotate synchronously. The driving member 6 and the stirring member 8 are linked by the transmission member 7. Through the specific structural design of the transmission member 7, the rotation axis is changed, and the stirring members 8 on the left and right sides can be driven to perform synchronous rotation and stirring operations at the same time, thereby improving the mixing efficiency.

[0044] The second guide sleeve 11, the second guide column 12, the second spring 13, and the baffle 14 are also included. The second guide sleeve 11 is fixed to the left and right sides of the inner bottom wall of the third mounting frame 81. The bottom end of the second guide column 12 passes through the second guide sleeve 11 and the bottom wall of the third mounting frame 81, and the baffle 14 is fixed. The second spring 13 is fixed between the baffle 14 and the third mounting frame 81. The second spring 13 is arranged around the second guide column 12. The stirring shaft 83 passes through the baffle 14, and the stirring blade 84 is located below the baffle 14. The baffle 14 is arranged to block the opening of the sample tube to prevent the sample from spilling out during the stirring, mixing and shaking up and down. The second spring 13 is arranged to ensure that the baffle 14 always blocks the opening of the sample tube during the up and down movement of the sample tube. Specifically, before the stirring operation, the linear module 10 drives the second mounting frame 9 and its upper structure to move downward. After the stirring blade 84 extends into the sample tube, the baffle 14 moves downward to resist the top of the sample tube. The linear module 10 continues to drive the second mounting frame 9 to move downward. At this time, the second spring 13 is in a contracted state until the stirring blade 84 moves into place. Under the action of the pusher 6, the sample tube moves downward. At this time, the second spring 13 extends, driving the baffle 14 to move downward to always block the opening of the sample tube. The second guide sleeve 11 and the second guide rod are provided to ensure the smooth up and down movement of the baffle 14.

[0045] The sample rack 16 is also included. The bottom wall of the sample rack 16 has a positioning hole 17 that cooperates with the positioning pin 15. The sample chamber 200, the solid phase extraction module 300, the oscillation module 400, the centrifugal module 500, the parallel concentration module 600, and the nitrogen blowing concentration module 700 all have positioning pins 15. The positioning pins 15 of the mixing device 401 are arranged on the left and right sides of the top wall of the first horizontal plate 51. Through the arrangement of the positioning pins 15 and the positioning holes 17, the sample rack 16 can be quickly positioned, the sample rack 16 can be quickly docked in each module, and the stability of the sample rack 16 after placement can be ensured. In particular, in the mixing device 401, the sample rack 16 is prevented from being separated from the first mounting frame 5 during the up and down reciprocating motion of the first mounting frame 5 by the arrangement of the positioning pins 15 and the positioning holes 17. And through the arrangement of the second spring 13 and the baffle 14, it can also be ensured that the sample tube and the sample rack 16 will not be separated during the up and down reciprocating motion of the first mounting frame 5.

[0046] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, it should be understood by those skilled in the art that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features thereof may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Sample pretreatment system, characterized by: It includes a transport module, and a sample compartment, a solid phase extraction module, an oscillation module, a centrifugation module, a parallel concentration module, and a nitrogen blowing concentration module distributed around the transport module; The transport module, sample chamber, solid phase extraction module, oscillation module, centrifugation module, parallel concentration module, and nitrogen blowing concentration module are independently arranged; The oscillation module has a mixing device, which includes a bottom plate, a first guide column, a first guide sleeve, a first spring, a first mounting frame, a pusher, a transmission member, a stirring member, a linear module, and a second mounting frame; First guide posts are fixed on both left and right sides of the top wall of the bottom plate, first guide sleeves are fixed on both left and right sides of the top wall of the first mounting frame, the first guide posts pass through the top wall and the first guide sleeve of the first mounting frame, a first spring is fixed between the bottom plate and the first mounting frame, and the first spring is arranged around the first guide posts; The linear module is fixed to the top wall of the base plate, and a second mounting frame is fixed to the sliding end of the linear module; The pushing member is fixed to the lower part of the second mounting frame, the stirring member is fixed to the upper part of the second mounting frame, and the pushing member is transmission-connected to the stirring member through a transmission member; The pushing member is used to push the first mounting frame to move downward.

2. The sample pretreatment system according to claim 1, characterized in that: The first mounting frame includes a first transverse plate, a vertical rod, and a second transverse plate; Vertical rods are fixed to the left and right sides of the bottom wall of the first horizontal plate, and the bottom ends of the vertical rods are fixed to the second horizontal plate; The pushing member is located above the second horizontal plate; The first guide sleeve is fixed to the top wall of the first transverse plate.

3. The sample pretreatment system according to claim 2, characterized in that: The pushing member includes a motor, a first rotating shaft, and a cam; A first rotating shaft is fixed to the front end of the motor, a cam is fixed to the front end of the first rotating shaft, and the cam is located above the second transverse plate.

4. The sample pretreatment system according to claim 3, characterized in that: The second mounting frame includes a third horizontal plate and a vertical plate; The third horizontal plate is fixed to the bottom end of the sliding end of the linear module, and the vertical plate is fixed to the bottom wall of the third horizontal plate; A stirring member is fixed on both the left and right sides of the bottom wall of the third horizontal plate; The motor is fixed to the front side wall at the lower part of the vertical plate.

5. The sample pretreatment system according to claim 4, characterized in that: The stirring member includes a third mounting frame, a first bearing seat, a stirring shaft, and a stirring blade; The third mounting frame is fixed to the bottom wall of the third horizontal plate; A first bearing seat is fixed in the middle of the inner bottom wall of the third mounting frame, and the bottom end of the stirring shaft passes through the first bearing seat and the bottom wall of the third mounting frame and extends to the bottom of the third mounting frame where a stirring blade is fixed.

6. The sample pretreatment system according to claim 5, characterized in that: Also includes a second guide sleeve, a second guide post, a second spring, and a baffle; Second guide sleeves are fixed to both left and right sides of the inner bottom wall of the third mounting frame, and the bottom end of the second guide column passes through the second guide sleeve and the bottom wall of the third mounting frame, and is fixed with a baffle; A second spring is fixed between the baffle and the third mounting frame, and the second spring is arranged around the second guide column; The stirring shaft passes through the baffle, and the stirring blade is located below the baffle.

7. The sample pretreatment system according to claim 5, characterized in that: The transmission member includes a first transmission wheel, a first transmission belt, a second transmission wheel, a second rotating shaft, a second bearing seat, a first bevel gear, a second bevel gear, a third rotating shaft, a third bearing seat, a double-groove transmission wheel, a third transmission wheel, and a second transmission belt; The first rotating shaft is fixed with a first transmission wheel, and the first transmission wheel is located at the rear side of the cam; A second bearing seat is fixed to the front side wall of the upper part of the vertical plate, and the rear end of the second rotating shaft is inserted into the second bearing seat; The second transmission wheel and the first bevel gear are both fixed to the second rotating shaft, the second transmission wheel is located at the front side of the first bevel gear, and the second transmission wheel is connected to the first transmission wheel through a first transmission belt; A third bearing seat is fixed to the bottom wall of the third horizontal plate, the top end of the third rotating shaft is inserted into the third bearing seat, a second bevel gear is fixed to the bottom end of the third rotating shaft, and the second bevel gear is meshed with the first bevel gear; A double-groove transmission wheel is fixed to the middle of the third rotating shaft, a third transmission wheel is fixed to the upper part of each stirring shaft, and the third transmission wheel is connected to the double-groove transmission wheel through a second transmission belt.

8. The sample pretreatment system according to claim 2, characterized in that: The sample chamber, solid phase extraction module, oscillation module, centrifugal module, parallel concentration module, and nitrogen blowing concentration module all have positioning pins; The positioning pins of the mixing device are arranged on the left and right sides of the top wall of the first transverse plate.

9. The sample pretreatment system according to claim 8, characterized in that: Also included is a sample rack, wherein the bottom wall of the sample rack has a positioning hole matched with the positioning pin.

10. A sample pretreatment method, characterized in that: The sample pretreatment system according to any one of claims 1 to 9 is used for processing, comprising the following steps: Initial sampling is performed in the sample bin. After the initial sampling is completed, the sample rack is transported to the oscillation module through the transfer module for mixed oscillation treatment. After the mixed oscillation treatment is completed, the sample rack is transported to the centrifugation module through the transfer module for centrifugation treatment. After the centrifugation treatment is completed, the sample rack is transported to the nitrogen blowing concentration module through the transfer module for nitrogen blowing concentration treatment. After the nitrogen blowing concentration treatment is completed, the sample rack is transported to the parallel concentration module through the transfer module for parallel concentration treatment. After the parallel concentration treatment is completed, the sample rack is transported to the solid phase extraction module through the transfer module for solid phase extraction treatment. After the solid phase extraction treatment is completed, the sample rack is transported back to the sample bin through the transfer module to complete the sample pre-treatment.

Images