Symbiotic planting and breeding process of grapes and rana nigromaculata

Through the grape-black-spotted frog symbiosis planting technology, the planting and breeding technology is optimized, the problems of insufficient land resource utilization and increased pressure on the ecological environment in traditional agricultural methods are solved, and the maximum utilization of resources and the improvement of economic benefits are achieved. .

CN119302262APending Publication Date: 2025-01-14ZHEJIANG DANSHUI FISHERY RESEARCH INSTITUTE (ZHEJIANG DANSHUI FISHERY ENVIRONMENTAL MONITORING STATION)
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Patent Information

Application Number
CN202411760703.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-03
Publication Date
2025-01-14

AI Technical Summary

Technical Problem

Traditional single crop cultivation and single animal breeding methods have led to insufficient utilization of land resources, increased ecological environment pressure, and resource waste and pollution problems.

Method used

Using the grape-black-spotted frog symbiotic planting technology, through reasonable planning of infrastructure, optimization of planting and breeding technology, we can maximize the utilization of land resources, reduce the occurrence of diseases, and improve economic benefits. Specific measures include trench depth design, spray cooling devices, grape trellis shading rate control, tail water treatment and biofertilizer recycling, etc.

Benefits of technology

It achieves the maximum utilization of land resources, reduces the occurrence of diseases, increases the yield and quality of grapes and black-spotted frogs, reduces production costs, increases economic benefits, realizes waste recycling, and protects the ecological environment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to a symbiotic planting and breeding process of grapes and rana nigromaculata, which realizes resource sharing and environment optimization of grape planting and rana nigromaculata breeding by reasonably utilizing land resources and ecological resources, improves the economic benefits of the grapes and the rana nigromaculata, and realizes symbiotic co-utilization. The technology comprises three aspects of capital construction, cultivation and planting, comprehensively considers the planting requirements of grapes and the growth environment of rana nigromaculata, realizes the maximum utilization of land resources and ecological resources through reasonable technology design and optimization, reduces the incidence rate of diseases, and improves economic benefits. The symbiotic planting and breeding technology covers the content from capital construction facility design, breeding technology implementation, planting technology implementation, management and maintenance and the like, efficient resource utilization and environmental protection are achieved by integrating the ecological characteristics of the rana nigromaculata and the grape planting requirements, remarkable economic and ecological benefits are achieved, and the method is suitable for large-scale popularization and application. And the dual purposes of environmental friendliness and cost saving are achieved, and the method has high application value and popularization prospects.
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Description

Technical Field

[0001] The invention relates to agricultural planting and breeding technology, in particular to a grape-black-spotted frog symbiotic planting and breeding process. Background Art

[0002] Traditional agricultural planting and breeding methods are often single-crop planting or single-animal breeding, resulting in insufficient utilization of land resources and increased pressure on the ecological environment. Single-crop planting often requires a large amount of fertilizers and pesticides to increase production, which not only increases production costs but also burdens the environment. Single animal breeding may have negative effects due to resource waste and pollution. With the development of agricultural planting and breeding technology, traditional single-crop planting and breeding methods have gradually exposed problems such as low resource utilization and low economic benefits. In order to improve land utilization efficiency and economic benefits, exploring the symbiotic model of multiple planting and breeding has become a current research hotspot. A reasonable symbiotic model can increase production, reduce the use of fertilizers and pesticides, and thus protect the ecological environment. Therefore, it is of great practical significance to develop a symbiotic breeding process that can simultaneously improve resource utilization efficiency and reduce environmental burden.

[0003] In recent years, my country's grape industry has developed rapidly and has become the world's largest grape producer and consumer. The rapid growth of grape scale and output has also brought a number of problems, such as the coexistence of phased saturation and structural saturation of grape market supply, great pressure on fruit farmers to increase their income, and relatively idle land and practitioners in winter. It is an important issue facing the grape industry to tap the potential of vineyard planting and find ways to increase output and benefits per unit area. Vigorously exploring and promoting the three-dimensional composite planting and breeding model of vineyards is a key choice to crack the development and revitalization of the grape industry. The vineyard planting and breeding combination model can make full use of idle space and land under the vineyard and improve the utilization rate of land, air and light.

[0004] The black-spotted frog has tender meat, delicious taste, and excellent taste. It is rich in nutrients such as protein, carbohydrates, creatine, and carnosine. It is a top-notch tonic and is loved by consumers. In recent years, due to environmental pollution and artificial killing, the number of wild black-spotted frogs has been decreasing. Therefore, many places have begun to artificially breed black-spotted frogs and have achieved good results. The breeding potential of black-spotted frogs is huge. Rational use of existing resources and careful breeding can effectively increase agricultural income.

[0005] As a traditional agricultural activity, grape cultivation mainly requires sufficient sunlight, suitable temperature and humidity. As an eco-friendly animal, the black-spotted frog's environmental requirements mainly include a moist growth environment and suitable shade conditions. Grapes and black-spotted frogs have complementary ecological needs: grape racks provide shade, and black-spotted frog feces can be used as natural fertilizer. The shade provided by the grape racks provides good habitat conditions for black-spotted frogs. At the same time, the excrement of black-spotted frogs contains rich organic matter, and the tail water produced during its breeding process can also be used as fertilizer after treatment, which is extremely beneficial to the growth of grapes. The vineyard planting and breeding combination model can make full use of the idle space and land under the vineyard, and improve the utilization rate of land, air, and light. Therefore, combining grape planting with black-spotted frog breeding and optimizing resource utilization through a symbiotic model can not only increase yields, but also reduce the use of fertilizers and pesticides, protect the ecological environment, and has broad application prospects. Summary of the invention

[0006] The purpose of the present invention is to provide a symbiotic cultivation and breeding process of grapes and black-spotted frogs, which can maximize the utilization of land resources, reduce the occurrence of diseases, and improve economic benefits by rationally planning infrastructure and optimizing planting and breeding processes. The process comprehensively considers the planting needs of grapes and the growth environment of black-spotted frogs, utilizes the symbiotic relationship to improve the utilization efficiency of land and resources, reduce the occurrence of diseases, and achieve the goals of green agriculture and circular economy. Specifically, the present invention relates to the design of groove depth, cooling of spray devices, control of shading rate of grape racks, extension of growth period, heat preservation and rain protection measures on rainy days, and recycling of tail water treatment and biofertilizer.

[0007] The above technical objectives of the present invention are achieved through the following technical solutions:

[0008] A grape-black-spotted frog symbiotic breeding process, comprising the following aspects:

[0009] (1) Infrastructure: Use fences and sky nets to prevent black-spotted frogs from escaping and invasion by natural enemies, set up well-designed trenches and drainage systems, set up feeding tables and insect traps on both sides of the trenches, and configure spray cooling devices;

[0010] (2) Breeding: Design shade systems, heat preservation measures, rain protection devices and sprinkler devices, strictly control the release and daily management of black-spotted frogs, and ensure the stable growth of black-spotted frogs under different climatic conditions;

[0011] (3) Planting: By adjusting the shading rate of the grape racks, the biological nutrients produced by drying the feces and tail water of the black-spotted frog are used as organic fertilizers for grape planting. In addition, the predatory behavior of the black-spotted frog is used for pest and disease control to improve the growth environment of the grapes.

[0012] In terms of infrastructure, 1.5-meter-high cement pillars were buried around the breeding area, and 1.2-meter-high polyethylene fine mesh fences were placed to prevent black-spotted frogs from escaping. Skynets 2 meters above the ground were set up above all grape racks to prevent birds and other animals from invading. Rational grooves, feeding tables and spraying devices were designed to optimize the living environment of black-spotted frogs and the growth conditions of grapes, so as to support the growth and activities of black-spotted frogs and provide a suitable growth environment for grapes.

[0013] Preferably, the groove depth is 30-50 cm, the width is 50-70 cm, an impermeable ground film is laid at the bottom of the groove, and water inlet and drainage pipes are provided to keep the water quality in the groove clean, and the water level is controlled between 15-40 cm. The construction of the groove is the first step in the implementation of the whole process. A straight groove type groove is set under the grape trellis in the planting area as a frog pond, with a straight groove in the middle and land on both sides as a habitat feeding area, and the design of the groove is optimized according to the biological characteristics of the black-spotted frog. The black-spotted frog grows in water for a long time, and its own excrement is much, and the water quality is easily polluted, causing hypoxia in the water or increasing harmful gases in the water, causing frog disease or even death. Problems such as insufficient groove height, too deep water, and large temperature difference between morning and evening will affect the growth of the black-spotted frog. Therefore, the groove depth setting needs to be able to support the jumping activities of the black-spotted frog, while ensuring that it has enough space for activities, and also ensuring the circulation and drainage of the water body. The water level of the black-spotted frog breeding trench should be maintained at 15-40 cm. Its depth is related to the water temperature. The water should be changed frequently to ensure good water quality and to ensure that the dissolved oxygen content in the water is above 3.5 mg / L. The depth design of the trench is adjusted according to the site conditions and the activity range of the black-spotted frog. The depth of the trench is set to 30-50 cm. The width of the trench should be moderate, not only to avoid narrowness that restricts the activity of the black-spotted frog, but also to prevent excessive width from affecting the humidity of its habitat and the growth of grapes. The trench width is set to 50-70 cm. Such a design can meet the habitat needs of the black-spotted frog without affecting the growth of grapes. The bottom of the trench needs to be treated to improve the comfort of the black-spotted frog and facilitate cleaning. An impermeable ground film is laid at the bottom of the trench to reduce water loss and pollution and keep the humidity of the grape growth environment appropriate. In order to improve the comfort of the black-spotted frog, appropriate bedding is laid at the bottom of the trench, which can absorb the excrement of the black-spotted frog, keep the trench hygienic, reduce odor, and reduce the difficulty of cleaning. At the same time, the bedding needs to be replaced regularly to ensure the cleanliness of the trench environment. The trench design also includes the setting of the drainage system. Install appropriate drainage pipes to prevent the water level in the trench from being too high and facilitate water changes. The drainage system can effectively remove excess water and maintain a suitable water level in the trench, clean up frog feces and grape leaves and other impurities in the trench in time, and ensure the control and management of water quality and water temperature when the black-spotted frog is active. The layout of the drainage pipes can ensure that every corner of the trench can be drained smoothly. Design water inlet and drainage pipes before and after the trench to ensure smooth and convenient inlet and outlet during breeding.

[0014] Preferably, the bottom layer of the trench is paved with an impermeable ground membrane, a cushion is laid on top of the impermeable ground membrane, an inlet pipe is installed above the front end of the trench, and an outlet pipe is installed above the rear end of the trench, and the geothermal pipe is buried at the bottom of the trench and below the impermeable ground membrane and the cushion.

[0015] Preferably, the food table is arranged on both sides of the groove, and is made of easy-to-clean stainless steel material. The height of the food table is 3-8 cm, and an insect trap system is provided next to the food table. The design of the food table is convenient for the staff to operate when feeding the feed, and a device for saving feed is required at the same time. The food table should be installed on both sides of the groove to facilitate the feeding of the black-spotted frog. The height of the food table should be set to 3-8 cm according to the activity habits of the black-spotted frog to ensure that it can easily take food. The surface of the food table should be smooth and easy to clean. A durable and easy-to-clean stainless steel material should be selected. The stainless steel material has corrosion resistance, can resist the acid and alkali substances in the feed, and has strong tolerance to weather temperature changes. The stainless steel material is easy to clean and keeps the breeding environment hygienic. The black-spotted frog lives on insects and mainly feeds on active insects. In order to improve the feeding efficiency and the vitality of the black-spotted frog, it is possible to consider installing an insect trap system. Live insects can be added to the feed while feeding. On the grape racks next to the feeding table, a 7-watt energy-saving lamp can be hung every 3-5 meters on the power supply and light wiring. Insects such as moths can be attracted at night to supplement the food source for black-spotted frogs, ensure the healthy development of black-spotted frogs, and improve the breeding efficiency. Black-spotted frogs feed on insects, and their predatory behavior can effectively reduce the incidence of diseases and pests in grapes, thereby reducing the use of pesticides. By observing the predatory behavior of black-spotted frogs through their ecological role, we can further optimize the pest and disease prevention and control strategy, reduce the occurrence of diseases and pests, and improve the yield and quality of grapes.

[0016] Preferably, the spray device is installed above the groove and is equipped with an automatic control system to adjust the spray time and water volume, and the spray flow rate is controlled at 5 to 10 liters / minute. The main function of the spray device is to adjust the growth temperature of the black-spotted frog. The spray device not only helps to reduce the ambient temperature, but also increases the air humidity and improves the growth environment of the black-spotted frog. The spray device is installed above the groove so that the entire groove area can be sprayed. The device is evenly distributed to ensure that the humidity inside and outside the groove can be effectively controlled. The spray head of the spray device can adjust the spray angle and range to ensure that the spray covers the entire breeding area. The spray device is equipped with an automatic control system to automatically adjust the spray time and water volume according to the ambient temperature and humidity to ensure that the black-spotted frog can be cooled in time in hot weather, and the spray flow rate is controlled at 5 to 10 liters / minute. The automatic control system can automatically open or close according to external climate changes to maintain a suitable humidity in the groove. The water source comes from groundwater or tap water, and is designed to be low-water consumption to reduce the waste of water resources.

[0017] Preferably, the shading material of the shading system is a mesh with good light transmittance, and the shading rate is controlled at 20%-60%. The shading rate can be adjusted according to the season to regulate the growth environment of the black-spotted frog and the light requirements of the grapes.

[0018] By setting up a shading device to adjust the growth environment of the black-spotted frog, the growth period of the black-spotted frog can be extended. The shading design takes into account the ecological needs of the black-spotted frog and the light needs of the grapes to improve the breeding efficiency. The shading system is designed according to the actual situation of the grape rack. The shading material is a mesh with good light transmittance. The shading rate is controlled between 20%-60%, which can provide a suitable shading environment for the black-spotted frog without affecting the photosynthesis of the grapes. The shading design also takes into account the changes in light in different seasons to ensure that the black-spotted frog and grapes can obtain a suitable environment at all growth stages. The shading system has an adjustment function to adapt to the changes in light in different seasons. Using an adjustable mesh, the degree of shading can be adjusted according to the light intensity. The system has two modes of manual and automatic adjustment to facilitate adjustments by operators.

[0019] As a preferred embodiment, the insulation measures include geothermal pipes, heating lamps, insulation materials and greenhouse technology to ensure that black-spotted frogs and grapes grow normally under low temperature conditions. In the autumn cooling period, black-spotted frogs and grapes need appropriate insulation measures. Insulation facilities include heating lamps and insulation materials, geothermal pipes and greenhouse technology to insulate the breeding area to ensure that black-spotted frogs will not get sick or affect growth due to lower temperatures, while protecting the root health of grapes. Geothermal pipes are buried under the grooves to provide stable temperatures for black-spotted frogs and grapes through geothermal conduction. Heating lamps are set above the grooves to increase light while providing temperature protection. Insulation materials and greenhouse technology cover the grape racks to form a closed insulation environment. Insulation facilities adjust the heating intensity and the thickness of insulation materials according to actual climatic conditions to maintain a suitable temperature range. The insulation system should be regularly inspected and maintained to ensure its normal operation. The inspection content includes the sealing of the pipelines, the structural integrity of the greenhouse, etc. Problems found should be repaired in time to ensure the insulation effect.

[0020] As preferably, the rainproof device comprises a canopy and a drainage system, the canopy covers above the groove, and the drainage system comprises a combination of ground drainage and groove drainage. Rainy days will affect the normal feeding of black-spotted frogs, so it is necessary to set a rainproof device on the grape rack. The rainproof device comprises a canopy and a drainage system, the canopy covers above the groove, prevents rainwater from directly falling into the groove to pollute the water quality, and prevents rainwater from falling into the food table to wet the feed, affecting the normal feeding of black-spotted frogs, ensuring that black-spotted frogs can also eat normally on rainy days. The design of the rainproof device also takes into account the discharge problem of rainwater, so the drainage system comprises a combination of ground drainage and groove drainage, to ensure that rainwater can be discharged smoothly, to avoid the negative impact of stagnant water on the environment. The rainproof device is regularly inspected and cleaned to ensure its normal operation. The inspection content includes the integrity of the canopy, the patency of the drainage pipe, etc. Finding problems should be handled in time to ensure the rainproof effect.

[0021] Preferably, the stocking amount of the black-spotted frog is 10,000 to 30,000 per mu, and the seedlings are young frogs. The climate at this time is suitable for the growth of young frogs, which can make them adapt to the new environment faster. The stocking amount of black-spotted frogs is 10,000 to 30,000 per mu, 160 per catty. In late March, young frogs with moderate size, strong vitality and no obvious injuries are selected for stocking to ensure the quality of the seedlings; the container containing the young frogs is placed at the edge of the breeding area, and the young frogs are slowly placed in the groove after 30-60 minutes of adaptation to the environmental conditions to avoid stress reactions in the young frogs due to sudden changes in the environment. The feed is fed every day the next day after being released for breeding management; the feeding management of black-spotted frogs includes regular feeding, cleaning of feces and checking of health status, regular inspection of the growth of black-spotted frogs, prevention of diseases, and timely treatment of diseases.

[0022] Choose appropriate feed according to the growth stage of the young frogs, and adjust the feeding amount reasonably according to the number of young frogs and their growth conditions. In the early stage, feed 2-5% of the total body weight of the young frogs, and gradually increase the feeding amount as the young frogs grow. Feed 2-3 times a day at regular intervals, in the morning, evening and night (when the light is turned on in conjunction with the insect trap system), and spread the feed evenly on the feeding table to ensure that every young frog has the opportunity to get food. At the same time, the insects trapped by the insect trap system provide a natural food source for the young frogs to enhance their physical fitness.

[0023] Regularly test the water quality in the ditch, clean up the feces of black-spotted frogs, change the water in time according to the water quality, keep the water clean, and ensure sufficient dissolved oxygen in the water; increase the frequency of water changes appropriately during high temperatures in summer to prevent high water temperatures from affecting the growth of young frogs; pay attention to keeping the water temperature stable in autumn to avoid faster changes in water temperature that may cause stagnation in the growth of young frogs; clean up impurities such as feces, feed residues, and fallen grape leaves at the bottom of the ditch in time to prevent water quality deterioration and bacterial growth.

[0024] Observe the black-spotted frog's activity, feeding habits, body surface characteristics, etc. regularly every morning and evening. If any abnormal phenomena such as slow movement, loss of appetite, skin damage or lesions are found, isolate them in time and conduct detailed examinations.

[0025] Preferably, the shading rate of the grape rack is controlled at 40% to 60% in summer, and adjusted to 20% to 30% in autumn when the leaves fall. The design of the grape rack takes into account the adjustment of the shading rate. The shading rate of the grape rack is controlled at 40% to 60% in summer to reduce the impact of high temperature on the grapes; in autumn, it is adjusted to 20% to 30% to increase the sunlight exposure time, shorten the hibernation period of the black-spotted frog, and extend its growth period.

[0026] Preferably, the excrement treatment of the black-spotted frog in the organic fertilizer includes composting and decomposing, and the tail water treatment includes sedimentation, filtration and drying. The excrement of the black-spotted frog is rich in organic matter and is a natural fertilizer for grape growth. Using the excrement of the black-spotted frog as an organic fertilizer for grape cultivation helps to improve soil structure and increase soil fertility. The excrement should be properly treated to ensure that it does not contain harmful substances. The biological nutrient fertilizer after tail water treatment can also be directly used for grape cultivation, with good fertilizer efficiency and environmental friendliness. The excrement of the black-spotted frog is used for grape cultivation after being properly treated, and the processing steps include composting and decomposing of the excrement; controlling humidity and temperature during the composting process to promote the decomposition of organic matter; the decomposed excrement is screened to remove impurities to ensure its fertilizer efficiency and safety. The tailwater produced during the breeding process of black-spotted frogs needs to be treated before it can be used for grape planting. The treatment steps include sedimentation, filtration and drying. The sedimentation process can remove solid impurities in the tailwater, the filtration process can remove tiny particles, and the drying process can remove water. The tailwater treatment system includes a sedimentation tank and drying equipment. The sedimentation tank is used to precipitate suspended matter in the tailwater. The treated clean water can be reused or discharged. The sludge in the sedimentation tank is dried and the generated biological nutrient fertilizer is used for grape planting. Use the biological nutrient fertilizer generated from the drying of the tailwater treatment end to fertilize the grapes, and adjust the fertilizer application according to the growth stage of the grapes and the nutrient requirements of the soil. Check the pH value and fertility of the soil regularly to ensure the healthy growth of the grapes.

[0027] In summary, the present invention has the following beneficial effects:

[0028] 1. The present invention realizes the maximum utilization of land resources and reduces the environmental burden by optimizing the infrastructure, breeding and planting processes through the symbiotic breeding process of grapes and black-spotted frogs. The breeding of black-spotted frogs and the planting of grapes cooperate with each other, which improves the utilization efficiency of resources, improves the yield and quality of grapes and black-spotted frogs, reduces production costs, and increases economic benefits.

[0029] 2. The application of technologies such as shade design, spray cooling devices, winter heat preservation measures, and rain protection measures ensure the stable growth of black-spotted frogs and grapes under different climatic conditions, greatly improving the success rate and economic benefits of breeding and planting;

[0030] 3. The biological fertilizer after the treatment of the black-spotted frog's feces and tail water is used as organic fertilizer for grape planting, realizing the recycling of waste, reducing the cost of chemical fertilizer use, and protecting the environment;

[0031] 4. The presence of black-spotted frogs can effectively reduce the occurrence of pests and diseases, thereby reducing the use of pesticides and improving the yield and quality of grapes;

[0032] 5. The present invention designs a symbiotic breeding process by comprehensively considering the ecological needs of grapes and black-spotted frogs, making full use of the complementary relationship between the two, which not only improves the efficiency of breeding and planting, but also realizes the recycling of waste. It adopts eco-friendly breeding and planting methods, reduces the negative impact on the environment, achieves the goal of sustainable development, and has broad market application prospects and promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a schematic diagram of the grape-black-spotted frog symbiotic breeding process;

[0034] Figure 2 is a schematic diagram of the groove cross section;

[0035] In the figure: 1. Anti-seepage ground membrane; 2. Geothermal pipe; 3. Water inlet pipe; 4. Water outlet pipe; 5. Pad material. DETAILED DESCRIPTION

[0036] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

[0037] The specific implementation method of the present invention covers the entire process from the planning and design of infrastructure to the implementation of the symbiotic cultivation and breeding process of grapes and black-spotted frogs. The process achieves the symbiotic benefits of grape planting and black-spotted frog breeding by optimizing the utilization of land resources and ecological resources. The process mainly covers three aspects: infrastructure, breeding and planting, aiming to improve resource utilization efficiency, reduce the incidence of diseases, and increase economic benefits. Specifically including:

[0038] 1. Infrastructure design:

[0039] The infrastructure is the core of the symbiotic farming process. 1.5-meter-high cement pillars are buried around the breeding area, and a 1.2-meter-high polyethylene fine mesh fence is placed to prevent black-spotted frogs from escaping. A sky net 2 meters above the ground is set above all grape racks to prevent birds and other animals from invading.

[0040] The infrastructure design mainly includes the design of trenches, feeding tables and sprinklers. Reasonable trenches, feeding tables and sprinklers are designed to optimize the living environment of black-spotted frogs and the growth conditions of grapes to support the growth and activities of black-spotted frogs and provide a suitable growth environment for grapes. In the planting area, trenches of specific depth and width are optimized according to the biological characteristics of black-spotted frogs to support the jumping activities of black-spotted frogs. Feeding tables are set on both sides of the trenches to facilitate feeding and save feed. At the same time, sprinklers are installed and equipped with automatic control systems to adjust the temperature to provide a comfortable growth environment for black-spotted frogs.

[0041] 1. Groove design:

[0042] The construction of the trench is the first step in the implementation of the entire process. A straight trench is set under the grape rack in the planting area as a frog breeding pond, with a straight trench in the middle and land on both sides as a habitat and feeding area, that is, feeding tables are set on both sides of the trench for feeding black-spotted frogs.

[0043] The design of the trench is optimized according to the biological characteristics of the black-spotted frog. The black-spotted frog lives in water for a long time and has a lot of excrement. The water quality is easily polluted, causing hypoxia in the water or increasing harmful gases in the water, causing frog diseases or even death. Problems such as insufficient trench height, too deep water, and large temperature difference between morning and evening will affect the growth of the black-spotted frog. Therefore, the trench depth setting needs to be able to support the jumping activities of the black-spotted frog, while ensuring that it has enough space for activities, and also ensuring the flow and drainage of the water body. The trench depth design is adjusted according to the site conditions and the range of activities of the black-spotted frog. The width of the trench should be moderate, avoiding narrowness that restricts the activities of the black-spotted frog, and preventing it from being too wide to affect the humidity of its habitat and the growth of grapes. An impermeable ground film is laid at the bottom of the trench to reduce water loss and pollution and keep the humidity of the grape growth environment appropriate. In order to improve the comfort of the black-spotted frog, appropriate bedding is laid at the bottom of the trench to reduce the difficulty of cleaning. Water inlet and drainage pipes are designed before and after the trench to ensure smooth and convenient inlet and outlet during breeding.

[0044] (1) Groove depth and width

[0045] The depth and width of the grooves are designed according to the biological characteristics of the black-spotted frog and the setting of the grape rack. The depth of the grooves is 30-50 cm and the width is 50-70 cm. This design can meet the habitat needs of the black-spotted frog without affecting the growth of the grapes.

[0046] (2) Trench bottom treatment

[0047] The bottom of the trench needs to be treated to improve the comfort of the black-spotted frog and facilitate cleaning. An impermeable ground film is laid at the bottom of the trench, and a certain thickness of bedding, such as hay or sawdust, is laid on top. These beddings can absorb and deposit the excrement of the black-spotted frog, keep the trench clean, and reduce odor. At the same time, the bedding needs to be replaced regularly to ensure the cleanliness of the trench environment.

[0048] (3) Drainage system

[0049] The design of the trench should take into account the setting of the drainage system. Install appropriate drainage pipes to prevent the water level in the trench from being too high and facilitate water changes. The drainage system can effectively remove excess water and maintain a suitable water level in the trench, promptly clean up frog feces and impurities such as grape leaves in the trench, and ensure the control and management of water quality and water temperature when the black-spotted frog is active. The layout of the drainage pipes can ensure that every corner of the trench can drain smoothly. The water level of the black-spotted frog breeding trench should be maintained at 15-40 cm, and its depth is related to the water temperature. The water should be changed frequently to ensure good water quality and ensure that the dissolved oxygen content in the water is above 3.5 mg / L.

[0050] The bottom layer of the trench is paved with an impermeable ground membrane 1, and a cushion material 5 is laid on the impermeable ground membrane 1. An inlet pipe 3 is installed above the front end of the trench, and an outlet pipe 4 is installed above the rear end of the trench. The geothermal pipe 2 is buried at the bottom of the trench and is located below the impermeable ground membrane 1 and the cushion material 5.

[0051] In the grape-black-spotted frog symbiotic breeding process, the design of the groove plays a key role in meeting the growth needs of the black-spotted frog and maintaining the stability of the entire ecosystem.

[0052] The trench has a certain depth and width, and its bottom structure is particularly important. The bottom layer is paved with an impermeable ground film 1, which effectively prevents water loss and lays the foundation for maintaining a suitable humidity for the grape growth environment. Pads 5 are laid on top of the impermeable ground film 1. Pads 5 can not only absorb impurities such as black-spotted frog excrement and reduce water pollution, but also further improve the cleanliness of the trench environment. The two work together to ensure the stability of the humidity of the trench environment and overall cleanliness.

[0053] The space in the trench is used to store water, which is one of the important conditions for meeting the growth needs of black-spotted frogs. The water level is controlled by the water inlet pipe 3 at the front end and the water outlet pipe 4 at the rear end. The water inlet pipe 3 is located above the front end of the trench, and its main function is to introduce fresh water to replenish water for the trench; the water outlet pipe 4 is set above the rear end of the trench and is responsible for discharging sewage and excess water. The two work together to accurately maintain the stability of the water level in the trench, ensure that the water quality is always in a suitable state, meet the strict requirements of the black-spotted frog for water quality, and ensure that the dissolved oxygen content in the water is above 3.5mg / L, thereby creating a good living environment for the black-spotted frog.

[0054] At the same time, considering the important influence of temperature on the growth of black-spotted frogs and grapes, a geothermal pipe 2 is also buried under the trench. The geothermal pipe 2 plays a key role under low temperature conditions. It can provide heat for the trench and surrounding areas, effectively maintain a suitable temperature, and ensure that black-spotted frogs and grapes can grow normally under different climatic conditions. From the perspective of positional relationship, the water inlet pipe 3 and the water outlet pipe 4 are located at the two ends of the trench, one in and one out, forming a complete water flow circulation control system, which mainly regulates the water flow in the horizontal direction; while the geothermal pipe 2 is located below the bottom of the trench, which is in sharp contrast to the water inlet and outlet pipes in the vertical direction. It mainly plays a temperature regulation function underground, and cooperates with the horizontal water flow control function of the water inlet and outlet pipes to jointly build a stable environment suitable for biological growth. In addition, the geothermal pipe 2 focuses on heating under the bottom of the trench, and complements the anti-seepage ground film 1 and padding 5 also located at the bottom of the trench. The anti-seepage diaphragm 1, as the basic defense line of the bottom layer, plays a key role in anti-seepage, while the padding 5 covering it plays a positive role in absorbing impurities, etc. The three complement each other and jointly ensure the stable operation of the entire symbiotic breeding system.

[0055] 2. Dining table design:

[0056] The design of the feeding table should be convenient for the staff to operate when feeding, and it should also be equipped with a device to save feed. The feeding table should be installed on both sides of the groove to facilitate feeding and avoid feed waste. The material of the feeding table should be easy to clean to maintain the hygiene of the breeding environment. Black-spotted frogs feed on insects, mainly active insects. Live insects can be added to the feed to ensure the healthy development of black-spotted frogs and improve breeding efficiency.

[0057] (1) Food table location

[0058] The feeding table should be set on both sides of the groove to facilitate the black-spotted frog to eat. The height of the feeding table should be set to 3-8 cm according to the activity habits of the black-spotted frog to ensure that it can easily eat.

[0059] (2) Food table materials

[0060] The surface of the feeding table should be smooth and easy to clean. Choose durable and easy-to-clean stainless steel material. Stainless steel is corrosion-resistant, can resist acid and alkali substances in feed, and is more tolerant to changes in weather temperature.

[0061] (3) Insect trapping system

[0062] In order to improve feeding efficiency and the vitality of black-spotted frogs, you can consider installing an insect trap system. On the grape racks next to the feeding table, connect the power supply and install a light line to hang a 7-watt energy-saving lamp every 3-5 meters. At night, it can attract insects such as moths and supplement the food source for black-spotted frogs.

[0063] 3. Spray device design:

[0064] The main function of the spray device is to adjust the growth temperature of the black-spotted frog. The spray device is installed above the trench and is equipped with an automatic control system, which automatically turns on according to changes in ambient temperature. The automatic control system can adjust the amount of water to ensure that the black-spotted frog can be cooled in time in hot weather. The design of the spray device should also consider the efficiency of water utilization to avoid unnecessary waste of water resources. The spray device not only helps to reduce the ambient temperature, but also increases the air humidity and improves the growth environment of the black-spotted frog. The nozzle of the spray device can adjust the spray angle and range to ensure that the spray covers the entire breeding area.

[0065] (1) Location of the sprinkler

[0066] The sprinkler is installed above the trench to spray the entire trench area. The sprinkler is evenly distributed to ensure that the humidity inside and outside the trench can be effectively controlled.

[0067] (2) Automatic control system

[0068] The sprinkler is equipped with an automatic control system, which automatically adjusts the spraying time and water volume according to the ambient temperature and humidity, and the spray flow rate is controlled at 5 to 10 liters per minute. The automatic control system can automatically open or close according to external climate changes to maintain appropriate humidity in the trench and reduce water waste.

[0069] (3) Water resource utilization

[0070] The water source should be a stable and reliable supply system, such as groundwater or tap water. The use of water sources adheres to the principle of conservation, so the sprinkler device is designed to be low-water consumption to avoid unnecessary waste of water resources.

[0071] 2. Implementation of breeding technology:

[0072] The breeding technology of black-spotted frogs is closely related to the growth of grapes, mainly involving shading, heat preservation, rain protection, etc. Design shading systems, heat preservation measures, rain protection devices and sprinkler devices, strictly control the release and daily management of black-spotted frogs, and ensure the stable growth of black-spotted frogs under different climatic conditions.

[0073] By setting up a shading device to adjust the growth environment of the black-spotted frog, the growth period of the black-spotted frog can be extended. The shading design takes into account the ecological needs of the black-spotted frog and the light needs of the grapes at the same time, improving the breeding efficiency. Insulation measures are adopted during the autumn temperature change period. The insulation measures use geothermal pipes and greenhouse technology to prevent the rapid temperature changes from affecting the growth of the black-spotted frog and grapes; rain protection measures are taken on rainy days. The rain protection measures include awnings and drainage systems, which do not affect the feeding of feed and ensure the stable growth of the black-spotted frog and grapes. In addition, a spray device is used to cool the black-spotted frog to ensure its growth environment in high temperature weather.

[0074] 1. Shade design:

[0075] The shading design of the grape rack should take into account the ecological needs of the black-spotted frog. The shading material of the shading system is a mesh with good light transmittance, and the shading rate is controlled between 20% and 60%, which can provide a suitable shading environment for the black-spotted frog without affecting the photosynthesis of the grapes. The shading design also takes into account the changes in light in different seasons to ensure that the black-spotted frog and grapes can obtain a suitable environment at all growth stages. The design of the grape rack takes into account the adjustment of the shading rate. In summer, the shading rate of the grape rack is controlled at 40% to 60% to reduce the impact of high temperature on grapes; in autumn, it is adjusted to 20% to 30% to increase the sunlight exposure time, shorten the hibernation period of the black-spotted frog, and extend its growth period.

[0076] (1) Shade system

[0077] The shading system is designed according to the actual situation of the grape rack. The shading rate is generally controlled between 20% and 60% to ensure the growth needs of black-spotted frogs and grapes. The shading material is a mesh with good light transmittance to ensure that the grapes can get enough light and provide a suitable habitat for black-spotted frogs.

[0078] (2) Adjustment of shading system

[0079] The shading system has an adjustable function to adapt to the changes in light in different seasons. The adjustable mesh can be used to adjust the degree of shading according to the light intensity. The system has two modes of manual and automatic adjustment to facilitate the operator to make adjustments.

[0080] 2. Insulation measures:

[0081] In winter, both black-spotted frogs and grapes need appropriate insulation measures. In autumn, insulation facilities include heating lamps and insulation materials, geothermal pipes and greenhouse technology to insulate the breeding area, ensure that the black-spotted frogs will not be affected by the rapid drop in temperature, and protect the health of the grape root system. Insulation facilities should adjust the heating intensity and the thickness of insulation materials according to actual climatic conditions to maintain a suitable temperature range.

[0082] (1) Insulation system design

[0083] The winter insulation system uses geothermal pipes, heating lamps, insulation materials and greenhouse technology. The geothermal pipes are buried under the trenches to provide a stable temperature for the black-spotted frogs and grapes through geothermal conduction. The heating lamps are set above the trenches to increase light and provide temperature protection. Insulation materials and greenhouse technology are covered above the grape racks to form a closed insulation environment.

[0084] (2) Maintenance of insulation system

[0085] The insulation system should be regularly inspected and maintained to ensure its normal operation. The inspection content includes the tightness of the pipes and the structural integrity of the greenhouse. If any problems are found, they should be repaired in time to ensure the insulation effect. During the autumn temperature change period, both black-spotted frogs and grapes need appropriate insulation measures.

[0086] 3. Rain protection measures:

[0087] Rainy days will affect the normal feeding of black-spotted frogs, so it is necessary to set up rainproof devices on the grape racks. The rainproof device includes a canopy and a drainage system. The canopy covers the top of the ditch to prevent rainwater from falling directly into the ditch and the feeding table, ensuring that black-spotted frogs can eat normally even on rainy days. The design of the rainproof device should take into account the discharge of rainwater, so the drainage system includes a combination of ground drainage and ditch drainage to avoid the negative impact of accumulated water on the environment.

[0088] (1) Rain protection device

[0089] The rain protection device includes a canopy and a drainage system. The canopy covers the top of the ditch to prevent rainwater from falling directly into the ditch and polluting the water quality, and also prevents rainwater from falling into the feeding table and wetting the feed, affecting the normal feeding of black-spotted frogs. The drainage system is designed to combine ground drainage and drainage in the ditch to ensure that rainwater can be discharged smoothly and prevent water accumulation from causing negative impacts on the environment.

[0090] (2) Management of rain protection system

[0091] The rainproof device should be inspected and cleaned regularly to ensure its normal operation. The inspection content includes the integrity of the awning, the patency of the drainage pipes, etc. If any problems are found, they should be dealt with in time to ensure the rainproof effect.

[0092] 4. Spraying device

[0093] The main function of the spray device is to lower the ambient temperature, increase the air humidity, and adjust the growth temperature of the black-spotted frog. The spray device is installed above the groove and is equipped with an automatic control system. It automatically turns on according to the changes in ambient temperature to ensure that the black-spotted frog can be cooled in time in hot weather.

[0094] (1) Spraying device

[0095] The spray device is installed above the trench to spray the entire trench area. The device is evenly distributed to ensure that the humidity inside and outside the trench can be effectively controlled. The nozzle of the spray device can adjust the spray angle and range to ensure that the spray covers the entire breeding area.

[0096] (2) Management of sprinkler devices

[0097] The sprinkler is equipped with an automatic control system, which automatically adjusts the spraying time and water volume according to the ambient temperature and humidity, and the spray flow rate is controlled at 5 to 10 liters per minute. The automatic control system can automatically open or close according to external climate changes to maintain appropriate humidity in the trench and reduce water waste.

[0098] 5. Stocking and management of black-spotted frogs

[0099] (1) Stocking of black-spotted frogs

[0100] The stocking quantity of black-spotted frogs is 10,000 to 30,000 per mu, 160 per jin. In late March, the seedlings are young frogs; select young frogs of moderate size, strong vitality, and no obvious injuries to ensure the quality of the seedlings; place the container with young frogs at the edge of the breeding area, and slowly put the young frogs into the ditch after 30-60 minutes of adapting to the environmental conditions to avoid stress reactions caused by sudden changes in the environment. Feed the young frogs every day from the next day for breeding management.

[0101] (2) Breeding and management of black-spotted frogs

[0102] The breeding and management of black-spotted frogs includes regular feeding, cleaning of feces and checking of health status, regular inspection of the growth of black-spotted frogs, prevention of disease occurrence, and timely handling of any disease problems that arise.

[0103] 3. Implementation of planting technology:

[0104] In terms of planting, the growth environment of grapes has been improved by adjusting the shading rate of the grape racks and adopting the drying technology at the tail water treatment end. The shading of the grape racks is designed to be 50%-70%, ensuring that the black-spotted frogs have a suitable shading environment without affecting the photosynthesis of the grapes. The shading rate of the grape racks is designed to extend the growth period of the black-spotted frogs and improve the breeding efficiency.

[0105] The symbiotic area is comprehensively utilized, and the symbiotic process is combined with the cultivation of black-spotted frogs through the grape planting process. The feces of black-spotted frogs are used as organic fertilizers, and the biological nutrient fertilizers after tailwater treatment are also used for grape planting. The fertilizers are properly processed to ensure their fertilizer efficiency and safety, realize the recycling of waste, reduce the use of chemical fertilizers, and achieve the dual goals of environmental friendliness and cost savings. At the same time, pest control is also optimized in combination with the predation behavior of black-spotted frogs.

[0106] 1. Fertilizer utilization:

[0107] The feces of black-spotted frogs are rich in organic matter and are natural fertilizers for grape growth. Using black-spotted frog feces as organic fertilizer for grape cultivation can help improve soil structure and increase soil fertility. The feces should be properly treated to ensure that they do not contain harmful substances. The biological nutrient fertilizer after tail water treatment can also be directly used for grape cultivation. This process includes steps such as sedimentation, filtration and drying of tail water. The fertilizer produced has good fertilizer efficiency and environmental friendliness.

[0108] (1) Feces treatment

[0109] The dung of the black-spotted frog is properly processed before being used in grape cultivation. The processing steps include composting and decomposition of the dung. During the composting process, the humidity and temperature are controlled to promote the decomposition of organic matter. After decomposition, the dung is screened to remove impurities to ensure its fertilizer efficiency and safety.

[0110] (2) Tailwater treatment

[0111] The tailwater produced during the breeding process of black-spotted frogs needs to be treated before being used in grape planting. The treatment steps include sedimentation, filtration and drying. The sedimentation process can remove solid impurities in the tailwater, the filtration process can remove tiny particles, and the drying process can remove water. The resulting fertilizer has good fertilizer efficiency and environmental friendliness. The tailwater treatment system includes a sedimentation tank and a drying equipment. The sedimentation tank is used to precipitate suspended matter in the tailwater, and the treated clean water can be reused or discharged. The sludge in the sedimentation tank is dried, and the generated biological nutrient fertilizer is used for grape planting. Use the biological nutrient fertilizer produced by drying from the tailwater treatment end to fertilize the grapes, and adjust the fertilizer application according to the growth stage of the grapes and the nutrient requirements of the soil. Check the pH value and fertility of the soil regularly to ensure the healthy growth of the grapes.

[0112] 2. Pest and disease control:

[0113] The presence of black-spotted frogs has a certain control effect on field pests. Black-spotted frogs feed on insects, which can effectively reduce the incidence of grape diseases and pests, thereby reducing the use of pesticides. By observing the predation behavior of black-spotted frogs through their ecological role, we can further optimize the pest control strategy, reduce the occurrence of pests and diseases, and improve the yield and quality of grapes.

[0114] (1) Predatory behavior of black-spotted frogs

[0115] The black-spotted frog feeds on insects, and its predation behavior can effectively reduce pests and diseases in vineyards. By observing the predation behavior of the black-spotted frog, we can understand its predation effect on different pests and adjust the prevention and control strategies.

[0116] (2) Comprehensive prevention and control measures

[0117] Combined with the predatory behavior of black-spotted frogs, comprehensive prevention and control measures are formulated. The measures include regular inspection of pests and diseases, rational use of biological control methods, and adjustment of grape cultivation management. These measures can effectively reduce the occurrence of pests and diseases and improve the yield and quality of grapes.

[0118] IV. Management and Maintenance

[0119] 1. Daily management

[0120] The fence outside the breeding area is to prevent the black-spotted frog from escaping, so it is necessary to check the fence frequently for holes or small holes, and to repair and plug them in time. At the same time, the fence and sky net can also prevent snakes, hedgehogs, weasels, etc. from entering the planting area, and prevent egrets, ospreys, water birds, sparrows, etc. from preying on black-spotted frogs or pecking grapes, so it is necessary to strengthen inspections and check the damage of the fence and sky net.

[0121] (1) Management of Black-spotted Frog

[0122] The daily management of black-spotted frogs includes monitoring the growth status of black-spotted frogs, checking their habitat, adjusting feed feeding, etc. Check the health status of black-spotted frogs regularly to prevent the occurrence of diseases and deal with any disease problems in a timely manner; regularly clean up impurities such as feces, feed residues, and fallen leaves in the ditch to ensure the water quality of the black-spotted frog's living environment; adjust the amount of feed according to the growth needs of black-spotted frogs, feed them regularly, and ensure their nutritional balance.

[0123] (2) Grape management

[0124] Grape management includes regular watering, fertilization, pruning, and pest and disease control. Adjust management measures according to the growth stage of the grapes to ensure their healthy growth. Adjust watering according to soil moisture and weather conditions, and apply fertilizer scientifically based on soil test results.

[0125] 2. Regular inspection

[0126] (1) Infrastructure inspection

[0127] Infrastructure facilities should be inspected regularly, including trenches, dining tables and sprinklers. The inspection contents include the cleanliness of trenches, the normal function of dining tables, the working condition of sprinklers, etc. If problems are found, they should be repaired in time to ensure the normal operation of the facilities.

[0128] (2) Environmental monitoring

[0129] Environmental monitoring includes factors such as temperature, humidity and light. Through the data of the monitoring system, environmental conditions can be adjusted in time to maintain the optimal growth state of black-spotted frogs and grapes. Environmental monitoring data should be recorded and analyzed regularly to evaluate the impact of environmental changes on breeding results.

[0130] 3. Data recording and analysis

[0131] (1) Data Recording

[0132] Keep detailed records of the growth of black-spotted frogs, the growth of grapes, environmental monitoring data, etc. The records include time, data values, observation results, etc. Data records should be timely and accurate to facilitate subsequent analysis and decision-making.

[0133] (2) Data analysis

[0134] Analyze the recorded data, including growth trend analysis, environmental factor analysis, pest and disease occurrence analysis, etc. Through data analysis, it is possible to evaluate the breeding effect, optimize management measures, and improve production efficiency.

[0135] V. Economic Benefit Analysis

[0136] 1. Cost Analysis

[0137] (1) Initial investment

[0138] The initial investment includes the construction cost of infrastructure, the purchase cost of black-spotted frogs, the purchase cost of grape seedlings, etc. Make a budget based on the actual situation and control the cost.

[0139] (2) Operating costs

[0140] Operating costs include feed costs, management costs, maintenance costs, etc. Make a budget based on the production scale and actual conditions, and arrange expenditures reasonably.

[0141] 2. Benefit Analysis

[0142] (1) Grape production

[0143] By implementing symbiotic cultivation and breeding technology, the yield of grapes can be increased. Yield statistics are regularly collected and compared with traditional cultivation methods to evaluate the yield improvement effect.

[0144] (2) Product quality

[0145] The quality of grapes can be improved by reducing pests and diseases and optimizing the cultivation process. Regular quality inspections are carried out and compared with traditional cultivation methods to evaluate the quality improvement effect.

[0146] (3) Economic benefits

[0147] Calculate economic benefits through cost and benefit analysis. Improving economic benefits can be achieved by reducing production costs, increasing product prices, and increasing output.

[0148] Example 1

[0149] (1) Infrastructure: A fence and sky net are used on the 1.17-mu land to prevent the black-spotted frog from escaping and the invasion of natural enemies. A well-designed ditch and drainage system are set up. Feeding tables and insect traps are set up on both sides of the ditch, and a spray cooling device is installed;

[0150] (2) Breeding: Design shade systems, heat preservation measures, rain protection devices and sprinkler devices, strictly control the release and daily management of black-spotted frogs, and ensure the stable growth of black-spotted frogs under different climatic conditions; the release rate of black-spotted frogs is 30,000 per mu, and 160 per kilo;

[0151] (3) Planting: By adjusting the shading rate of the grape racks, the biological nutrients produced by drying the feces and tailwater of the black-spotted frog are used as organic fertilizers for grape planting. In combination with the predatory behavior of the black-spotted frog, pest and disease control is carried out to improve the growth environment of grapes. The grape variety planted is Black Emperor, with a planting density of 56 plants per mu.

[0152] Economic benefits: output of black-spotted frogs 45,000 yuan / mu, grapes 20,000 yuan / mu, and comprehensive benefits of more than 60,000 yuan / mu.

[0153] Example 2

[0154] (1) Infrastructure: 1.26 acres of land will be covered with fences and sky nets to prevent black-spotted frogs from escaping and natural enemies from invading. A well-designed ditch and drainage system will be set up. Feeding tables and insect traps will be set up on both sides of the ditch, and a spray cooling device will be installed.

[0155] (2) Breeding: Design shade systems, heat preservation measures, rain protection devices and sprinkler devices, strictly control the release and daily management of black-spotted frogs, and ensure the stable growth of black-spotted frogs under different climatic conditions; the release rate of black-spotted frogs is 20,000 per mu, and 160 per kilo;

[0156] (3) Planting: By adjusting the shading rate of the grape racks, the biological nutrients produced by drying the feces and tailwater of the black-spotted frog are used as organic fertilizers for grape planting. In combination with the predatory behavior of the black-spotted frog, pest and disease control is carried out to improve the growth environment of grapes. The grape variety planted is Black Emperor, with a planting density of 56 plants per mu.

[0157] Economic benefits: output of black-spotted frogs 30,000 yuan / mu, grapes 20,000 yuan / mu, and comprehensive benefits of more than 50,000 yuan / mu.

[0158] Example 3

[0159] (1) Infrastructure: 1.80 mu of land is covered with fences and sky nets to prevent black-spotted frogs from escaping and invasion by natural enemies. A well-designed ditch and drainage system is set up. Feeding tables and insect traps are set up on both sides of the ditch, and a spray cooling device is installed;

[0160] (2) Breeding: Design shade systems, heat preservation measures, rain protection devices and sprinkler devices, strictly control the release and daily management of black-spotted frogs, and ensure the stable growth of black-spotted frogs under different climatic conditions; the release rate of black-spotted frogs is 10,000 per mu, and 160 per kilo;

[0161] (3) Planting: By adjusting the shading rate of the grape racks, the biological nutrients produced by drying the feces and tailwater of the black-spotted frog are used as organic fertilizers for grape planting. In combination with the predatory behavior of the black-spotted frog, pest and disease control is carried out to improve the growth environment of grapes. The grape variety planted is Black Emperor, with a planting density of 56 plants per mu.

[0162] Economic benefits: output of black-spotted frogs 15,000 yuan / mu, grapes 20,000 yuan / mu, and comprehensive benefits of more than 35,000 yuan / mu.

[0163] Comparative Example 1

[0164] The Black Emperor grapes were planted using traditional planting methods on an area of ​​0.68 acres of land, with a planting density of 56 plants per acre; the black-spotted frogs were raised using traditional breeding methods on an area of ​​0.62 acres of land, with a stocking rate of 10,000 per acre and 160 per pound.

[0165] Economic benefits: output of black-spotted frogs is 9,000 yuan / mu, and output of grapes is 11,000 yuan / mu, with comprehensive benefits exceeding 10,000 yuan / mu.

[0166] Result analysis:

[0167] From the comparison results of the economic benefit data of grapes and black-spotted frogs in Examples 1-3 and Comparative Example 1, it can be seen that the economic benefits per mu in Examples 1-3 of the present invention are greater than that in Comparative Example 1, indicating that the grape-black-spotted frog symbiotic breeding process provided by the present invention has excellent economic value.

Claims

1. A grape-black-spotted frog symbiotic breeding process, characterized in that: These include: (1) Infrastructure: Use fences and sky nets to prevent black-spotted frogs from escaping and invasion by natural enemies, set up well-designed trenches and drainage systems, set up feeding tables and insect traps on both sides of the trenches, and configure spray cooling devices; (2) Breeding: Design shade systems, heat preservation measures, rain protection devices and sprinkler devices, strictly control the release and daily management of black-spotted frogs, and ensure the stable growth of black-spotted frogs under different climatic conditions; (3) Planting: By adjusting the shading rate of the grape racks, the biological nutrients produced by drying the feces and tail water of the black-spotted frog are used as organic fertilizers for grape planting. In addition, the predatory behavior of the black-spotted frog is used for pest and disease control to improve the growth environment of the grapes.

2. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The trench is 30-50 cm deep and 50-70 cm wide. An impermeable diaphragm is laid at the bottom of the trench and it is equipped with water inlet and drainage pipes to keep the water in the trench clean and the water level controlled between 15-40 cm.

3. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The bottom layer of the trench is paved with an impermeable ground film (1), a cushion material (5) is laid on the impermeable ground film (1), an inlet pipe (3) is installed above the front end of the trench, and an outlet pipe (4) is installed above the rear end of the trench, while the geothermal pipe (2) is buried at the bottom of the trench and is located below the impermeable ground film (1) and the cushion material (5).

4. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The feeding table is set on both sides of the groove and is made of easy-to-clean stainless steel material. The height of the feeding table is 3-8 cm, and an insect attraction system is installed next to the feeding table. The spraying device is installed above the groove and is equipped with an automatic control system to adjust the spraying time and water volume. The spray flow rate is controlled at 5 to 10 liters / minute.

5. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The shading material of the shading system is a mesh with good light transmittance, and the shading rate is controlled at 20%-60%. The shading rate can be adjusted according to the season to adjust the growth environment of the black-spotted frog and the light demand of the grapes.

6. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The insulation measures include geothermal pipes, heating lamps, insulation materials and greenhouse technology to ensure the normal growth of black-spotted frogs and grapes under low temperature conditions.

7. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The rain protection device comprises a rain shelter and a drainage system. The rain shelter covers the grooves, and the drainage system comprises a combination of ground drainage and groove drainage.

8. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The stocking amount of the black-spotted frog is 10,000 to 30,000 per mu, and the stocking is carried out in late March, and the seedlings are young frogs; young frogs with moderate size, strong vitality and no obvious injuries are selected for stocking to ensure the quality of the seedlings; the container with the young frogs is placed at the edge of the breeding area, and the young frogs are slowly placed in the ditch after acclimatizing to the environmental conditions for 30 to 60 minutes to avoid stress reactions of the young frogs due to sudden changes in the environment, and feed is fed every day from the next day after being released for breeding management; the feeding management of the black-spotted frog includes regular feeding, cleaning of feces and checking of health status, regular inspection of the growth of the black-spotted frog, prevention of disease occurrence, and timely treatment of disease problems.

9. A grape-black-spotted frog symbiotic breeding process according to claim 1 or 5, characterized in that: The shading rate of the grape rack is controlled at 40% to 60% in summer and adjusted to 20% to 30% in autumn.

10. The grape-black-spotted frog symbiotic breeding process according to claim 1, characterized in that: The treatment of the excrement of the black-spotted frog in the organic fertilizer includes composting and decomposing, and the treatment of the tail water includes sedimentation, filtration and drying.

Citation Information

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