Chlorella fermented feed based on fish culture and preparation method thereof

Abstract

The present invention relates to the technical field of chlorella fermented feeds, and discloses a fish culture-based chlorella fermented feed, which comprises the following raw materials by weight: 60-70% of chlorella dry powder, 20-25% of soybean meal, 10-15% of fish meal, 5-10% of a yeast extract, 1-3% of a trace element premix, 2-4% of a vitamin premix, and 0.5-1% of a leavening agent. According to the chlorella fermented feed based on fish culture and the preparation method thereof, microorganisms in the leavening agent can decompose cell walls of chlorella, the step is the key for improving the digestion and absorption rate, and decomposition of the cell walls releases internal nutritional ingredients such as protein and essential amino acid, so that the chlorella fermented feed is easier to digest and absorb by fishes; in addition, microbial metabolites, such as organic acid and enzyme, generated in the fermentation process can further promote absorption of the fish to the nutritional ingredients, and by means of the technical scheme, the bioavailability of the chlorella is remarkably improved, so that the utilization efficiency of the fish to the nutritional ingredients in the feed is improved.

Description

Technical Field

[0001] The invention relates to the technical field of chlorella fermented feed, and in particular to a chlorella fermented feed based on fish farming and a preparation method thereof. Background Art

[0002] In traditional fish farming, the use of feed has always been a key factor restricting the development of the industry. These feeds often face multiple problems such as high cost, scarce resources and burden on the environment. They not only increase the financial burden of the aquaculture industry, but also cause concerns in terms of resource sustainability and ecological protection.

[0003] However, the emergence of Chlorella offers an innovative solution to this challenge. This single-cell green algae is valued for its high protein content. Its rapid growth ability means that large amounts of biomass can be produced in a short period of time, providing a renewable resource for feed production. Chlorella is rich in protein and contains all the essential amino acids required for fish growth, making it an ideal feed ingredient in theory.

[0004] However, there is a major obstacle when using Chlorella directly: its digestion and absorption rate is relatively low, which is mainly due to the toughness of the Chlorella cell wall and the low bioavailability of its internal nutrients. In order to overcome this limitation, a Chlorella fermented feed based on fish farming and a preparation method are proposed. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the shortcomings of the prior art, the present invention provides a Chlorella fermented feed based on fish farming and a preparation method, which has the advantages of high nutritional value, easy digestion, environmental protection and significant economic benefits, and solves a major obstacle when Chlorella is used directly: its digestion and absorption rate is relatively low, which is mainly due to the toughness of the Chlorella cell wall and the low bioavailability of its internal nutrients.

[0007] (II) Technical solution

[0008] In order to achieve the above-mentioned purposes of high nutritional value, easy digestion, environmental protection and significant economic benefits, the present invention provides the following technical scheme: a chlorella fermented feed based on fish farming, comprising the following raw materials in weight proportions: 60-70% chlorella dry powder, 20-25% soybean meal, 10-15% fish meal, 5-10% yeast extract, 1-3% trace element premix, 2-4% vitamin premix and 0.5-1% starter.

[0009] A method for preparing a chlorella fermented feed based on fish farming, comprising the chlorella fermented feed based on fish farming, and further comprising the following steps:

[0010] S1. Raw material preparation and proportioning: all raw materials are accurately weighed according to the specified percentages, including 60-70% chlorella dry powder, 20-25% soybean meal, 10-15% fish meal, 5-10% yeast extract, 1-3% trace element premix, 2-4% vitamin premix and 0.5-1% starter culture;

[0011] S2. Mixing and fermentation: Mix 60-70% of chlorella dry powder, 20-25% of soybean meal, 10-15% of fish meal and 5-10% of yeast extract in a double-screw conical mixer to ensure uniform mixing. After the mixing is completed, pour the mixture into a sealed fermentation tank, and add 0.5-1% of fermentation agent into the sealed fermentation tank;

[0012] S3. Addition of minerals and vitamins: After fermentation is completed, the mixture is first stabilized, and then 1-3% of trace element premix and 2-4% of vitamin premix are added to the mixture;

[0013] S4, drying and granulation: the mixture is secondary dried using a spray dryer, and then made into feed pellets with a diameter of 3 mm using a ring die granulator;

[0014] S5. Quality testing: Sample feed pellets and conduct quality testing to ensure that the product meets the predetermined quality standards.

[0015] Preferably, the preparation process of the Chlorella dry powder comprises the following steps:

[0016] H1. Cultivating Chlorella vulgaris in a closed photobioreactor, the closed photobioreactor is equipped with an LED light source, the light intensity is set to 5500 l x, the light cycle is set to 12 hours light / 12 hours dark, and the water temperature is maintained at 25°C, and the pH value of the water is maintained within the range of 7.0 by adding one of CO2 and NaHCO3;

[0017] H2. The chlorella is collected by a high-speed centrifuge, the speed of the high-speed centrifuge is set to 3500 rpm, the time is set to 12 minutes, and then the chlorella is dried by a flash dryer, the inlet temperature of the flash dryer is set to 180° C., the outlet temperature is set to 80° C., and the moisture content of the dried chlorella powder is less than 8%.

[0018] Preferably, the trace element premix includes iron, copper, zinc, manganese, iodine, selenium, cobalt and chromium, and the vitamin premix is ​​prepared by mixing vitamin A, vitamin D3, vitamin E, vitamin K and vitamin B group.

[0019] Preferably, the mixing time of the double-helix cone mixer is 40-60 minutes, the fermentation temperature of the sealed fermenter is set to 37° C., the humidity is set to 75%, and the fermentation time is not less than 48 hours.

[0020] Preferably, the stabilization treatment of the mixture refers to adding a stabilizer to the mixture, wherein the stabilizer is sodium ascorbate, and the added amount is 0.15% of the total weight of the main raw material.

[0021] Preferably, the air inlet temperature of the spray dryer is set to 180° C., the air outlet temperature is set to 75° C., and the moisture content of the feed particles after drying is ensured to be less than 6%.

[0022] Preferably, the quality inspection process of feed pellets includes:

[0023] S501, Sample collection: Randomly collect samples on the production line to ensure that the samples can represent the quality of the entire batch of products;

[0024] S502, physical property testing: measure the diameter, uniformity and moisture content of feed particles to ensure that the particle size meets the standard and the moisture content is less than 6%;

[0025] S503. Nutritional component analysis: Determine the content of protein, fat, fiber, ash, trace elements and vitamins in feed by chemical analysis methods;

[0026] S504, Microbiological safety assessment: Conduct microbiological tests on samples, including the activity of probiotics and the number of harmful microorganisms, to ensure product safety;

[0027] S505, Stability and Nutritional Value Testing: Conduct accelerated stability testing and nutritional value assessment, including digestibility and bioavailability of nutrients;

[0028] S506, Quality Audit and Feedback: Compare the test results with the predetermined standards, conduct quality audits, and feedback the results to the production team so that necessary adjustments and improvements can be made.

[0029] (III) Beneficial effects

[0030] Compared with the prior art, the present invention provides a chlorella fermented feed based on fish farming and a preparation method thereof, which has the following beneficial effects:

[0031] 1. The chlorella fermented feed based on fish farming and the preparation method significantly improve the digestion and absorption rate of chlorella through the fermentation process. In the preparation method, the chlorella dry powder is first mixed with other raw materials and evenly mixed in a double-helix conical mixer, and then the mixture is poured into a sealed fermentation tank, and a fermentation agent is added for fermentation. The microorganisms in the fermentation agent can effectively decompose the cell wall of chlorella. This step is the key to improving the digestion and absorption rate. The decomposition of the cell wall releases the internal nutrients, such as protein and essential amino acids, making it easier for fish to digest and absorb. In addition, the microbial metabolites produced during the fermentation process, such as organic acids and enzymes, can further promote the absorption of nutrients by fish. Through this technical solution, the bioavailability of chlorella is significantly improved, thereby improving the utilization efficiency of nutrients in feed by fish.

[0032] 2. The chlorella fermented feed based on fish farming and the preparation method thereof, through fermentation and stabilization treatment, the chlorella fermented feed not only improves the nutritional value, but also reduces the dependence on traditional feed raw materials, thereby reducing the production cost. In the stabilization treatment step, sodium ascorbate is added to the mixture as a stabilizer, which helps to maintain the stability of the nutrients after fermentation and prevent the loss of nutrients in the subsequent treatment process. In addition, the entire production process can be carried out in a closed system, reducing the impact on the environment, meeting the requirements of green farming and sustainable development. This environmentally friendly production method, combined with the improvement of economic benefits, makes the present invention of great significance in promoting the sustainable development of the fish farming industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The schematic diagram of the preparation process of the fermented Chlorella feed based on fish farming of the present invention;

[0034] Figure 2 The schematic diagram of the preparation process of the Chlorella dry powder of the present invention;

[0035] Figure 3 The figure is a schematic diagram of the quality detection process of feed pellets of the present invention. DETAILED DESCRIPTION

[0036] The following will be combined with the embodiments of the present invention and the accompanying drawings to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] Embodiment 1:

[0038] A chlorella fermented feed based on fish farming comprises the following raw materials in proportion by weight: 60% chlorella dry powder, 20% soybean meal, 10% fish meal, 5% yeast extract, 1% trace element premix, 2% vitamin premix and 0.5% starter.

[0039] A method for preparing a chlorella fermented feed based on fish farming, comprising the chlorella fermented feed based on fish farming, and further comprising the following steps:

[0040] S1. Raw material preparation and proportion: all raw materials are accurately weighed according to the specified percentages, including 60% chlorella powder, 20% soybean meal, 10% fish meal, 5% yeast extract, 1% trace element premix, 2% vitamin premix and 0.5% starter;

[0041] S2, mixing and fermentation: mixing 60% of chlorella dry powder, 20% of soybean meal, 10% of fish meal and 5% of yeast extract in a double-screw conical mixer to ensure uniform mixing. After the mixing is completed, pour the mixture into a sealed fermentation tank, and add 0.5% of the fermentation agent into the sealed fermentation tank;

[0042] S3. Addition of minerals and vitamins: After fermentation is completed, the mixture is first stabilized, and then 1% of trace element premix and 2% of vitamin premix are added to the mixture;

[0043] S4, drying and granulation: the mixture is secondary dried using a spray dryer, and then made into feed pellets with a diameter of 3 mm using a ring die granulator;

[0044] S5. Quality testing: Sample feed pellets and conduct quality testing to ensure that the product meets the predetermined quality standards.

[0045] Specifically, the preparation process of Chlorella dry powder includes the following steps:

[0046] H1. Cultivating Chlorella vulgaris in a closed photobioreactor, the closed photobioreactor is equipped with an LED light source, the light intensity is set to 5500 l x, the light cycle is set to 12 hours light / 12 hours dark, and the water temperature is maintained at 25°C, and the pH value of the water is maintained within the range of 7.0 by adding one of CO2 and NaHCO3;

[0047] H2. The chlorella is collected by a high-speed centrifuge, the speed of the high-speed centrifuge is set to 3500 rpm, the time is set to 12 minutes, and then the chlorella is dried by a flash dryer, the inlet temperature of the flash dryer is set to 180° C., the outlet temperature is set to 80° C., and the moisture content of the dried chlorella powder is less than 8%.

[0048] Specifically, the trace element premix includes iron, copper, zinc, manganese, iodine, selenium, cobalt and chromium, and the vitamin premix is ​​prepared by mixing vitamin A, vitamin D3, vitamin E, vitamin K and vitamin B group.

[0049] Specifically, the mixing time of the double-helix conical mixer is 40-60 minutes, the fermentation temperature of the sealed fermentation tank is set to 37° C., the humidity is set to 75%, and the fermentation time is not less than 48 hours.

[0050] Specifically, the stabilization treatment of the mixture refers to adding a stabilizer to the mixture, wherein the stabilizer is sodium ascorbate, and the added amount is 0.15% of the total weight of the main raw material.

[0051] Specifically, the air inlet temperature of the spray dryer is set to 180° C., the air outlet temperature is set to 75° C., and the moisture content of the dried feed particles is ensured to be less than 6%.

[0052] Specifically, the quality inspection process of feed pellets includes:

[0053] S501, Sample collection: Randomly collect samples on the production line to ensure that the samples can represent the quality of the entire batch of products;

[0054] S502, physical property testing: measure the diameter, uniformity and moisture content of feed particles to ensure that the particle size meets the standard and the moisture content is less than 6%;

[0055] S503. Nutritional component analysis: Determine the content of protein, fat, fiber, ash, trace elements and vitamins in feed by chemical analysis methods;

[0056] S504, Microbiological safety assessment: Conduct microbiological tests on samples, including the activity of probiotics and the number of harmful microorganisms, to ensure product safety;

[0057] S505, Stability and Nutritional Value Testing: Conduct accelerated stability testing and nutritional value assessment, including digestibility and bioavailability of nutrients;

[0058] S506, Quality Audit and Feedback: Compare the test results with the predetermined standards, conduct quality audits, and feedback the results to the production team so that necessary adjustments and improvements can be made.

[0059] In this embodiment, the fish farming-based Chlorella fermented feed prepared according to the above formula and process has a small formula component and a low proportion of Chlorella dry powder, which helps to reduce production costs while maintaining necessary nutrients. It is suitable for markets or application scenarios that are more sensitive to costs. The lower proportion of starter agent means that less microorganisms are used in the fermentation process, which helps to control fermentation costs.

[0060] Embodiment 2:

[0061] A chlorella fermented feed based on fish farming comprises the following raw materials in proportion by weight: 65% chlorella dry powder, 22% soybean meal, 12% fish meal, 7% yeast extract, 2% trace element premix, 3% vitamin premix and 0.7% starter.

[0062] A method for preparing a chlorella fermented feed based on fish farming, comprising the chlorella fermented feed based on fish farming, and further comprising the following steps:

[0063] S1. Raw material preparation and proportion: all raw materials are accurately weighed according to the specified percentages, including 65% chlorella powder, 22% soybean meal, 12% fish meal, 7% yeast extract, 2% trace element premix, 3% vitamin premix and 0.7% starter culture;

[0064] S2. Mixing and fermentation: Mix 65% of chlorella powder, 22% of soybean meal, 12% of fish meal and 7% of yeast extract in a double-screw conical mixer to ensure uniform mixing. After the mixing is completed, pour the mixture into a sealed fermentation tank, and add 0.7% of the fermentation agent into the sealed fermentation tank;

[0065] S3. Addition of minerals and vitamins: After fermentation is completed, the mixture is first stabilized, and then 2% of trace element premix and 3% of vitamin premix are added to the mixture;

[0066] S4, drying and granulation: the mixture is secondary dried using a spray dryer, and then made into feed pellets with a diameter of 3 mm using a ring die granulator;

[0067] S5. Quality testing: Sample feed pellets and conduct quality testing to ensure that the product meets the predetermined quality standards.

[0068] Specifically, the preparation process of Chlorella dry powder includes the following steps:

[0069] H1. Cultivating Chlorella vulgaris in a closed photobioreactor, the closed photobioreactor is equipped with an LED light source, the light intensity is set to 5500 l x, the light cycle is set to 12 hours light / 12 hours dark, and the water temperature is maintained at 25°C, and the pH value of the water is maintained within the range of 7.0 by adding one of CO2 and NaHCO3;

[0070] H2. The chlorella is collected by a high-speed centrifuge, the speed of the high-speed centrifuge is set to 3500 rpm, the time is set to 12 minutes, and then the chlorella is dried by a flash dryer, the inlet temperature of the flash dryer is set to 180° C., the outlet temperature is set to 80° C., and the moisture content of the dried chlorella powder is less than 8%.

[0071] Specifically, the trace element premix includes iron, copper, zinc, manganese, iodine, selenium, cobalt and chromium, and the vitamin premix is ​​prepared by mixing vitamin A, vitamin D3, vitamin E, vitamin K and vitamin B group.

[0072] Specifically, the mixing time of the double-helix conical mixer is 40-60 minutes, the fermentation temperature of the sealed fermentation tank is set to 37° C., the humidity is set to 75%, and the fermentation time is not less than 48 hours.

[0073] Specifically, the stabilization treatment of the mixture refers to adding a stabilizer to the mixture, wherein the stabilizer is sodium ascorbate, and the added amount is 0.15% of the total weight of the main raw material.

[0074] Specifically, the air inlet temperature of the spray dryer is set to 180° C., the air outlet temperature is set to 75° C., and the moisture content of the dried feed particles is ensured to be less than 6%.

[0075] Specifically, the quality inspection process of feed pellets includes:

[0076] S501, Sample collection: Randomly collect samples on the production line to ensure that the samples can represent the quality of the entire batch of products;

[0077] S502, physical property testing: measure the diameter, uniformity and moisture content of feed particles to ensure that the particle size meets the standard and the moisture content is less than 6%;

[0078] S503. Nutritional component analysis: Determine the content of protein, fat, fiber, ash, trace elements and vitamins in feed by chemical analysis methods;

[0079] S504, Microbiological safety assessment: Conduct microbiological tests on samples, including the activity of probiotics and the number of harmful microorganisms, to ensure product safety;

[0080] S505, Stability and Nutritional Value Testing: Conduct accelerated stability testing and nutritional value assessment, including digestibility and bioavailability of nutrients;

[0081] S506, Quality Audit and Feedback: Compare the test results with the predetermined standards, conduct quality audits, and feedback the results to the production team so that necessary adjustments and improvements can be made.

[0082] In this embodiment, the fermented Chlorella feed for fish farming prepared according to the above formula and process has moderate formula components and a moderate ratio of raw materials, which provides a better nutritional balance and helps to improve the digestion and absorption rate of fish to the feed, thereby improving the growth performance of fish. The moderate ratio of trace elements and vitamin premixes helps to ensure the comprehensiveness and balance of nutrients in the feed, meet the growth and development needs of fish, and the moderate increase in the proportion of the starter helps to more effectively decompose the cell wall of Chlorella and improve the bioavailability of nutrients.

[0083] Embodiment three:

[0084] A chlorella fermented feed based on fish farming comprises the following raw materials in proportion by weight: 70% chlorella dry powder, 25% soybean meal, 15% fish meal, 10% yeast extract, 3% trace element premix, 4% vitamin premix and 1% starter.

[0085] A method for preparing a chlorella fermented feed based on fish farming, comprising the chlorella fermented feed based on fish farming, and further comprising the following steps:

[0086] S1. Raw material preparation and proportion: all raw materials are accurately weighed according to the specified percentages, including 70% chlorella powder, 25% soybean meal, 15% fish meal, 10% yeast extract, 3% trace element premix, 4% vitamin premix and 1% starter;

[0087] S2. Mixing and fermentation: Mix 70% of chlorella powder, 25% of soybean meal, 15% of fish meal and 10% of yeast extract in a double-screw conical mixer to ensure uniform mixing. After the mixing is completed, pour the mixture into a sealed fermentation tank, and add 1% of the fermentation agent into the sealed fermentation tank;

[0088] S3. Addition of minerals and vitamins: After fermentation is completed, the mixture is first stabilized, and then 3% of trace element premix and 4% of vitamin premix are added to the mixture;

[0089] S4, drying and granulation: the mixture is secondary dried using a spray dryer, and then made into feed pellets with a diameter of 3 mm using a ring die granulator;

[0090] S5. Quality testing: Sample feed pellets and conduct quality testing to ensure that the product meets the predetermined quality standards.

[0091] Specifically, the preparation process of Chlorella dry powder includes the following steps:

[0092] H1. Cultivating Chlorella vulgaris in a closed photobioreactor, the closed photobioreactor is equipped with an LED light source, the light intensity is set to 5500 l x, the light cycle is set to 12 hours light / 12 hours dark, and the water temperature is maintained at 25°C, and the pH value of the water is maintained within the range of 7.0 by adding one of CO2 and NaHCO3;

[0093] H2. The chlorella is collected by a high-speed centrifuge, the speed of the high-speed centrifuge is set to 3500 rpm, the time is set to 12 minutes, and then the chlorella is dried by a flash dryer, the inlet temperature of the flash dryer is set to 180° C., the outlet temperature is set to 80° C., and the moisture content of the dried chlorella powder is less than 8%.

[0094] Specifically, the trace element premix includes iron, copper, zinc, manganese, iodine, selenium, cobalt and chromium, and the vitamin premix is ​​prepared by mixing vitamin A, vitamin D3, vitamin E, vitamin K and vitamin B group.

[0095] Specifically, the mixing time of the double-helix conical mixer is 40-60 minutes, the fermentation temperature of the sealed fermentation tank is set to 37° C., the humidity is set to 75%, and the fermentation time is not less than 48 hours.

[0096] Specifically, the stabilization treatment of the mixture refers to adding a stabilizer to the mixture, wherein the stabilizer is sodium ascorbate, and the added amount is 0.15% of the total weight of the main raw material.

[0097] Specifically, the air inlet temperature of the spray dryer is set to 180° C., the air outlet temperature is set to 75° C., and the moisture content of the dried feed particles is ensured to be less than 6%.

[0098] Specifically, the quality inspection process of feed pellets includes:

[0099] S501, Sample collection: Randomly collect samples on the production line to ensure that the samples can represent the quality of the entire batch of products;

[0100] S502, physical property testing: measure the diameter, uniformity and moisture content of feed particles to ensure that the particle size meets the standard and the moisture content is less than 6%;

[0101] S503. Nutritional component analysis: Determine the content of protein, fat, fiber, ash, trace elements and vitamins in feed by chemical analysis methods;

[0102] S504, Microbiological safety assessment: Conduct microbiological tests on samples, including the activity of probiotics and the number of harmful microorganisms, to ensure product safety;

[0103] S505, Stability and Nutritional Value Testing: Conduct accelerated stability testing and nutritional value assessment, including digestibility and bioavailability of nutrients;

[0104] S506, Quality Audit and Feedback: Compare the test results with the predetermined standards, conduct quality audits, and feedback the results to the production team so that necessary adjustments and improvements can be made.

[0105] In this embodiment, the fermented chlorella feed based on fish farming prepared according to the above formula and process has a large formula component, and a high proportion of chlorella dry powder provides more abundant protein and other nutrients, which helps to improve the growth rate and health level of fish. The higher ratio of soybean meal and fish meal increases the nutritional value of the feed, especially in the content of essential amino acids, which has a positive effect on the muscle development of fish. The increased proportion of trace elements and vitamin premixes helps to provide more comprehensive trace nutrients to support the immune system and overall health of fish.

[0106] In summary, the fermented Chlorella feed based on fish farming and the preparation method significantly improve the digestion and absorption rate of Chlorella through the fermentation process. In the preparation method, the Chlorella dry powder is first mixed with other raw materials and evenly mixed in a double-helix conical mixer, and then the mixture is poured into a sealed fermentation tank, and a fermentation agent is added for fermentation. The microorganisms in the fermentation agent can effectively decompose the cell wall of Chlorella. This step is the key to improving the digestion and absorption rate. The decomposition of the cell wall releases the internal nutrients, such as protein and essential amino acids, making it easier for fish to digest and absorb. In addition, the microbial metabolites produced during the fermentation process, such as organic acids and enzymes, can further promote the absorption of nutrients by fish. Through this technical solution, the bioavailability of Chlorella is significantly improved, thereby improving the utilization efficiency of nutrients in feed by fish.

[0107] Moreover, the chlorella fermented feed based on fish farming and the preparation method thereof, through fermentation and stabilization treatment, the chlorella fermented feed not only improves the nutritional value, but also reduces the dependence on traditional feed raw materials, thereby reducing the production cost. In the stabilization treatment step, sodium ascorbate is added to the mixture as a stabilizer, which helps to maintain the stability of the nutrients after fermentation and prevent the loss of nutrients in the subsequent treatment process. In addition, the entire production process can be carried out in a closed system, reducing the impact on the environment, meeting the requirements of green farming and sustainable development. This environmentally friendly production method, combined with the improvement of economic benefits, makes the present invention of great significance in promoting the sustainable development of the fish farming industry, and solves a major obstacle of chlorella when it is used directly: its digestion and absorption rate is relatively low, which is mainly due to the toughness of the chlorella cell wall and the low bioavailability of its internal nutrients.

[0108] The relevant modules involved in this system are all hardware system modules or functional modules that combine computer software programs or protocols with hardware in the prior art. The computer software programs or protocols involved in the functional modules are themselves technologies that are well known to those skilled in the art and are not improvements of this system. The improvements of this system are the interaction or connection relationships between the modules, that is, improvements to the overall structure of the system to solve the corresponding technical problems to be solved by this system.

[0109] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A chlorella fermented feed based on fish farming, characterized in that: The invention comprises the following raw materials in proportion by weight: 60-70% of chlorella dry powder, 20-25% of soybean meal, 10-15% of fish meal, 5-10% of yeast extract, 1-3% of trace element premix, 2-4% of vitamin premix and 0.5-1% of fermentation agent.

2. A method for preparing a fermented Chlorella feed based on fish farming, characterized in that: The fish farming-based Chlorella fermented feed according to claim 1 further comprises the following steps: S1. Raw material preparation and proportioning: all raw materials are accurately weighed according to the specified percentages, including 60-70% chlorella dry powder, 20-25% soybean meal, 10-15% fish meal, 5-10% yeast extract, 1-3% trace element premix, 2-4% vitamin premix and 0.5-1% starter culture; S2. Mixing and fermentation: Mix 60-70% of chlorella dry powder, 20-25% of soybean meal, 10-15% of fish meal and 5-10% of yeast extract in a double-screw conical mixer to ensure uniform mixing. After the mixing is completed, pour the mixture into a sealed fermentation tank, and add 0.5-1% of fermentation agent into the sealed fermentation tank; S3. Addition of minerals and vitamins: After fermentation is completed, the mixture is first stabilized, and then 1-3% of trace element premix and 2-4% of vitamin premix are added to the mixture; S4, drying and granulation: the mixture is secondary dried using a spray dryer, and then made into feed pellets with a diameter of 3 mm using a ring die granulator; S5. Quality testing: Sample feed pellets and conduct quality testing to ensure that the product meets the predetermined quality standards.

3. The chlorella fermented feed based on fish farming according to claim 1, characterized in that: The preparation process of the chlorella dry powder comprises the following steps: H1. Cultivating Chlorella in a closed photobioreactor, the closed photobioreactor is equipped with an LED light source, the light intensity is set to 5500 lx, the light cycle is set to 12 hours light / 12 hours dark, and the water temperature is maintained at 25°C, and the water pH is maintained within the range of 7.0 by adding one of CO2 and NaHCO3; H2. The chlorella is collected by a high-speed centrifuge, the speed of the high-speed centrifuge is set to 3500 rpm, the time is set to 12 minutes, and then the chlorella is dried by a flash dryer, the inlet temperature of the flash dryer is set to 180° C., the outlet temperature is set to 80° C., and the moisture content of the dried chlorella powder is less than 8%.

4. The fermented Chlorella feed based on fish farming according to claim 1, characterized in that: The trace element premix comprises iron, copper, zinc, manganese, iodine, selenium, cobalt and chromium, and the vitamin premix is ​​prepared by mixing vitamin A, vitamin D3, vitamin E, vitamin K and vitamin B group.

5. The method for preparing a fermented Chlorella feed based on fish farming according to claim 1, characterized in that: The mixing time of the S2 double-helix conical mixer is 40-60 minutes, the fermentation temperature of the sealed fermentation tank is set to 37° C., the humidity is set to 75%, and the fermentation time is not less than 48 hours.

6. The method for preparing a fermented Chlorella feed based on fish farming according to claim 1, characterized in that: The stabilization treatment of the mixture in S3 refers to adding a stabilizer to the mixture, wherein the stabilizer is sodium ascorbate, and the added amount is 0.15% of the total weight of the main raw material.

7. The method for preparing a fermented Chlorella feed based on fish farming according to claim 1, characterized in that: In the step S4, the air inlet temperature of the spray dryer is set to 180° C., the air outlet temperature is set to 75° C., and the moisture content of the dried feed particles is ensured to be less than 6%.

8. The method for preparing a fermented Chlorella feed based on fish farming according to claim 1, characterized in that: S5, the quality inspection process of feed pellets includes: S501, Sample collection: Randomly collect samples on the production line to ensure that the samples can represent the quality of the entire batch of products; S502, physical property testing: measure the diameter, uniformity and moisture content of feed particles to ensure that the particle size meets the standard and the moisture content is less than 6%; S503. Nutritional component analysis: Determine the content of protein, fat, fiber, ash, trace elements and vitamins in feed by chemical analysis methods; S504, Microbiological safety assessment: Conduct microbiological tests on samples, including the activity of probiotics and the number of harmful microorganisms, to ensure product safety; S505, Stability and Nutritional Value Testing: Conduct accelerated stability testing and nutritional value assessment, including digestibility and bioavailability of nutrients; S506, Quality Audit and Feedback: Compare the test results with the predetermined standards, conduct quality audits, and feedback the results to the production team so that necessary adjustments and improvements can be made.

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