Composite polypeptide tightening essence and preparation method thereof

Abstract

The invention discloses composite polypeptide firming essence and a preparation method thereof.The essence is prepared from, by weight, 3%-5% of humectant, 0%-1% of antioxidant, 0.3%-5% of skin conditioner and the balance purified water, the skin conditioner is a mixture of a skin conditioner a, a skin conditioner b and a skin conditioner c, and particularly, the skin conditioner a, the skin conditioner b and the skin conditioner c are mixed to form the composite polypeptide firming essence. And the skin conditioner c adopts an effective combination of an anti-aging composite 9 peptide composition and various firming and anti-wrinkle composite polypeptides, permeation of the composite polypeptides is promoted from multiple directions and multiple target points, multi-layer cell reconstruction and regeneration are given to the skin, the skin integrity is enhanced, a thickening agent and a preservative do not need to be added into the product, and the product has the advantages of being easy to absorb, free of side effects and high in activity. By controlling the preparation method, the antioxidant can be added or not added into the raw materials, and the obtained product has almost no difference in performance.

Description

Technical Field

[0001] The invention relates to a skin care product, in particular to an essence liquid for skin care and a preparation method thereof. Background Art

[0002] The continuous deepening of the exploration of the secrets of human aging provides the necessary nutrients for skin growth, builds the skin microecology to form a self-protection membrane, can effectively prevent foreign pathogens and harmful bacteria from entering the skin surface, and can also secrete excess oil to clean up the garbage on the skin surface. The biological aesthetic perspective will be sublimated from the macro to the micro level of cell and tissue regulation. The main basic functional unit cells of the human body are the basis for affecting the occurrence, development and aging of the skin. The health of skin cells depends on internal metabolism and external nutrition supply.

[0003] As people's living standards continue to improve, the market for cosmetics continues to improve, and people's requirements for skin care products are also constantly increasing. In order to meet market demand, companies also need to keep pace with the times through scientific research and development and process technology innovation to create more new generation skin care products to meet market demand; research and development pursues simplicity, process upgrading, and precise positioning. The purpose of this application's targeted effect is to develop a complex polypeptide firming essence that effectively solves skin aging problems such as dry and rough skin, fine lines, dry lines, and sagging. Summary of the invention

[0004] In order to overcome the shortcomings of the prior art in skin problems such as skin sagging, fine lines, dry lines, and aging, the present invention provides a composite polypeptide firming essence and a preparation method thereof.

[0005] The first technical solution adopted by the present invention to solve its technical problem is: A composite polypeptide firming essence containing an antioxidant is prepared from the following raw materials in percentage by weight: 3%-5% of a moisturizer, 0.1%-1% of an antioxidant, 0.3%-5% of a skin conditioner, and the remainder being purified water, wherein the skin conditioner is a mixture of three substances, skin conditioner a, skin conditioner b and skin conditioner c, in any proportion; the skin conditioner a is one or a mixture of two or more of dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-5 in any proportion; the skin conditioner b is one or a mixture of two or more of acetyl hexapeptide-8, acetyl hexapeptide-1 and acetyl hexapeptide-3 in any proportion; and the skin conditioner c is an anti-aging composite 9-peptide composition.

[0006] The anti-aging composite 9-peptide composition is made of the following raw materials in weight percentage: 10% acetyl hexapeptide-8, 10% dipeptide diaminobutyryl benzylamide diacetate, 20% carnosine, 10% glutathione, 10% oligopeptide-1, 10% dipeptide-2, 10% tripeptide-1 copper, 10% acetyl dipeptide-1 cetyl ester, and 10% tripeptide-32.

[0007] The moisturizing agent is one of glycerol, propylene glycol, sodium hyaluronate, and 1,2-hexanediol, or a mixture of two or more of them in any proportion.

[0008] The antioxidant is p-hydroxyacetophenone.

[0009] The steps of preparing the antioxidant-containing composite polypeptide firming essence are as follows: (1) Add the above amount of moisturizer and purified water into an emulsifier, emulsify and homogenize for 1-2 minutes, then stir and heat to 85°C, keep stirring for 20 minutes until completely dissolved.

[0010] (2) Add the above amount of antioxidant and stir for 5-10 minutes until completely dissolved.

[0011] (3) Cool down to 45°C, add the above amount of skin conditioner, and stir for 5-10 minutes until completely dissolved.

[0012] (4) After cooling to room temperature, take a sample for inspection. After passing the micro-inspection, take out the material and package it to obtain a complex polypeptide firming essence containing antioxidants.

[0013] The second technical solution adopted by the present invention to solve its technical problem is: A composite polypeptide firming essence without antioxidants is made of the following raw materials in percentage by weight: 3%-5% moisturizer, 0.3%-5% skin conditioner, and the rest is purified water, wherein the skin conditioner is a mixture of three substances, skin conditioner a, skin conditioner b and skin conditioner c, in any proportion. The skin conditioner a is one or a mixture of two or more of dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7, and acetyl tetrapeptide-5 in any proportion. The skin conditioner b is one or a mixture of two or more of acetyl hexapeptide-8, acetyl hexapeptide-1, and acetyl hexapeptide-3 in any proportion.

[0014] The skin conditioning agent C is an anti-aging composite 9-peptide composition, which is made of the following raw materials in weight percentage: acetyl hexapeptide-8 10%, dipeptide diaminobutyryl benzylamide diacetate 10%, carnosine 20%, glutathione 10%, oligopeptide-1 10%, dipeptide-2 10%, tripeptide-1 copper 10%, acetyl dipeptide-1 cetyl ester 10%, and tripeptide-32 10%.

[0015] The moisturizing agent is one of glycerol, propylene glycol, sodium hyaluronate, and 1,2-hexanediol, or a mixture of two or more of them in any proportion.

[0016] The steps of preparing the composite polypeptide firming essence without antioxidants are as follows: (1) Add the above amount of moisturizer and purified water into an emulsifying pot, heat to 85°C, and stir for 15 minutes until completely dissolved to obtain phase A.

[0017] (2) Cool down to 35-45℃, filter and sterilize, then take samples for inspection. After passing the micro-inspection, phase B can be obtained.

[0018] (3) Take an appropriate amount of phase B, mix phase B and skin conditioning agent in a reaction kettle, and then filter to obtain phase C.

[0019] (4) Add phase C to the remaining phase B, stir and mix evenly, and then aseptically package to obtain a complex polypeptide firming essence without antioxidants.

[0020] The beneficial effects of the present invention are as follows: the skin conditioner in the formula of the present invention is a combined formula, in particular, an anti-aging composite 9-peptide composition is adopted, and a plurality of firming and anti-wrinkle composite polypeptides are effectively combined, the composite polypeptides are promoted to penetrate from multiple directions and multiple targets, and the skin is endowed with multi-level cell reconstruction and regeneration, the skin integrity is enhanced, the skin firmness and elasticity are increased, the skin aging is effectively resisted, and diversified long-term moisturizing and skin firming are achieved, and no thickener and preservative need to be added to the product, so that the product has the advantages of easy absorption, no side effects and high activity, and the skin cell function is regulated through multiple targets to keep the skin in a healthy and vibrant youthful state.

[0021] By controlling the preparation method, the present invention can choose to add antioxidants to the raw materials or remove antioxidant components, and the obtained product performance is almost the same. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0023] Figure 1 It is a schematic diagram of the inhibition rate of the test substance of the present invention on the activity of elastase. DETAILED DESCRIPTION

[0024] The present invention can add antioxidants to the raw materials or remove antioxidant components through the control of the preparation method, and the obtained product performance is almost the same. The raw materials of the present invention can be purchased directly from the market, and the performance of raw materials from different manufacturers is not much different, and the overall performance of the product is not affected.

[0025] A composite polypeptide firming essence containing an antioxidant is prepared from the following raw materials in percentage by weight: 3%-5% of a moisturizer, 0.1%-1% of an antioxidant, 0.3%-5% of a skin conditioner, and the remainder being purified water (solvent), wherein the skin conditioner is a mixture of three substances, skin conditioner a, skin conditioner b and skin conditioner c, in any proportion; the skin conditioner a is one or a mixture of two or more of dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-5 in any proportion; the skin conditioner b is one or a mixture of two or more of acetyl hexapeptide-8, acetyl hexapeptide-1 and acetyl hexapeptide-3 in any proportion; and the skin conditioner c is an anti-aging composite 9-peptide composition.

[0026] In this embodiment, the antioxidant is p-hydroxyacetophenone. The anti-aging composite 9-peptide composition is made of the following raw materials in weight percentage: acetyl hexapeptide-8 10%, dipeptide diaminobutyryl benzylamide diacetate 10%, carnosine 20%, glutathione 10%, oligopeptide-1 10%, dipeptide-2 10%, tripeptide-1 copper 10%, acetyl dipeptide-1 cetyl ester 10%, tripeptide-32 10%. The moisturizer is one or a mixture of any proportion of glycerol, propylene glycol, sodium hyaluronate, and 1,2-hexanediol.

[0027] The steps of preparing the antioxidant-containing composite polypeptide firming essence are as follows: (1) Add the above amount of moisturizer and purified water into the emulsifier, emulsify and homogenize for 1-2 minutes (there is no strict requirement, this range can be satisfied), then stir and heat to 85℃, keep warm and stir for 20 minutes until completely dissolved. There is no strict requirement for the stirring speed, just operate according to the instructions of the equipment.

[0028] (2) Add the above amount of antioxidant, stir at warm temperature for 5-10 minutes (there is no strict requirement, this range can be satisfied) until completely dissolved.

[0029] (3) Cool down to 45°C, add the above amount of skin conditioner, and stir for 5-10 minutes (there is no strict requirement, this range can be satisfied) until it is completely dissolved.

[0030] (4) After cooling to room temperature, take a sample for inspection. After passing the micro-inspection, take out the material and package it to obtain a complex polypeptide firming essence containing antioxidants.

[0031] Four specific implementation methods are given below: Example 1 Components and weights: Humectant: Glycerin 3%.

[0032] Antioxidant: p-Hydroxyacetophenone 0.1%.

[0033] Skin Conditioners Skin conditioning agent a: Palmitoyl pentapeptide-4 0.1%.

[0034] Skin conditioning agent b: Acetyl hexapeptide-1 0.1%.

[0035] Skin conditioning agent c: Acetyl hexapeptide-8 0.01%, dipeptide diaminobutyroyl benzylamide diacetate 0.01%, carnosine 0.02%, glutathione 0.01%, oligopeptide-1 0.01%, dipeptide-2 0.01%, tripeptide-1 copper 0.01%, acetyl dipeptide-1 cetyl ester 0.01%, tripeptide-32 0.01%.

[0036] Example 2 Components and weights: Humectants: Glycerin 3%, Propylene Glycol 3%, Sodium Hyaluronate 1%, 1,2-Hexanediol 1%.

[0037] Antioxidant: p-Hydroxyacetophenone 0.5%.

[0038] Skin Conditioners Skin conditioning agent a: dipeptide diaminobutyroyl benzylamide diacetate 0.1%, palmitoyl tripeptide-1 0.1%, palmitoyl tripeptide-8 0.3%, palmitoyl tetrapeptide-7 0.5%.

[0039] Skin conditioning agent b: acetyl hexapeptide-8 0.2%, acetyl hexapeptide-1 0.2%, acetyl hexapeptide-3 0.2%.

[0040] Skin conditioning agent c: Acetyl hexapeptide-8 0.1%, dipeptide diaminobutyroyl benzylamide diacetate 0.1%, carnosine 0.2%, glutathione 0.1%, oligopeptide-1 0.1%, dipeptide-2 0.1%, tripeptide-1 copper 0.1%, acetyl dipeptide-1 cetyl ester 0.1%, tripeptide-32 0.1%.

[0041] Example 3 Components and weights: Humectants: Glycerin 6%, Propylene Glycol 6%, Sodium Hyaluronate 2%.

[0042] Antioxidant: p-Hydroxyacetophenone 0.8%.

[0043] Skin Conditioners Skin conditioning agent a: dipeptide diaminobutyroyl benzylamide diacetate 0.1%, palmitoyl pentapeptide-4 0.1%, palmitoyl tripeptide-1 0.2%, palmitoyl tripeptide-5 0.2%, palmitoyl tripeptide-8 0.3%, acetyl tetrapeptide-5 0.3%, palmitoyl tetrapeptide-7 0.3%, acetyl tetrapeptide-5 0.5%.

[0044] Skin conditioning agent b: acetyl hexapeptide-8 0.2%, acetyl hexapeptide-3 0.3%.

[0045] Skin conditioning agent c: Acetyl hexapeptide-8 0.1%, dipeptide diaminobutyroyl benzylamide diacetate 0.1%, carnosine 0.2%, glutathione 0.1%, oligopeptide-1 0.1%, dipeptide-2 0.1%, tripeptide-1 copper 0.1%, acetyl dipeptide-1 cetyl ester 0.1%, tripeptide-32 0.1%.

[0046] Example 4 Components and weights: Humectants: Glycerin 6%, Propylene Glycol 6%, Sodium Hyaluronate 3%, 1,2-Hexanediol 5%.

[0047] Antioxidant: p-Hydroxyacetophenone 1%.

[0048] Skin Conditioners Skin conditioning agent a: palmitoyl pentapeptide-4 0.1%, palmitoyl tripeptide-1 0.1%, palmitoyl tripeptide-5 0.2%, acetyl tetrapeptide-5 0.5%, acetyl tetrapeptide-5 0.1%.

[0049] Skin conditioning agent b: acetyl hexapeptide-8 0.3%, acetyl hexapeptide-1 0.2%, acetyl hexapeptide-3 0.5%.

[0050] Skin conditioning agent c: acetyl hexapeptide-8 0.3%, dipeptide diaminobutyroyl benzylamide diacetate 0.3%, carnosine 0.6%, glutathione 0.3%, oligopeptide-1 0.3%, dipeptide-2 0.3%, tripeptide-1 copper 0.3%, acetyl dipeptide-1 cetyl ester 0.3%, tripeptide-32 0.3%.

[0051] The antioxidant-containing composite polypeptide firming essence prepared in Examples 1-4 was tested, and the stability was observed after being stored at room temperature for 6 months. The antioxidant-containing composite polypeptide firming essence prepared in Examples 1-4 had no turbidity, precipitation, or sedimentation, indicating good stability.

[0052] Experimental test: The products of the above Examples 1-4 were mixed and tested as a sample group.

[0053] 1. Test Principle Elastin plays a key role in the youthfulness of the skin, and the lack of elastin is the main cause of skin aging. Inhibiting the activity of elastase can effectively slow down the decomposition of elastin and maintain the elasticity of the skin. Elastase can catalyze the hydrolysis of hydroxyl groups contained in the polypeptide bonds of various amino acids. Therefore, when it reacts with the substrate N-succinyl-alanine-alanine-alanine-p-nitroaniline, it will produce colored substances, and its absorbance can be measured at 410nm in a spectrophotometer. According to the change in absorbance, the inhibitory effect of the test substance on the activity of elastase is calculated, and whether the test substance has firming and anti-wrinkle effects can be evaluated.

[0054] 2. Materials and Methods 1. Instruments and Equipment One-tenth precision balance, microplate ELISA reader.

[0055] 2. Reagents and Materials Elastase, AAAPAN: N-succinyl-alanine-alanine-alanine-p-nitroanilide, positive control: EGCG, buffer: Tris-HCl pH 8.0.

[0056] 3. Operation process 3.1 Test operation Elastase and the chromogenic substrate N-succinyl-alanine-alanine-alanine-p-nitroaniline were prepared into working solutions using a buffer solution and prepared immediately before use.

[0057] The positive control EGCG was prepared with buffer and diluted to a series of concentrations to verify the test system. Test substance treatment: The test substance was diluted with buffer to the required test concentration, and no dilution was required if no requirement was met.

[0058] According to the sample liquid addition grouping list, the test was carried out using a 96-well microplate to set up a sample group (T), a sample background group (To), an enzyme reaction group (C) and a solvent background group (Co). The positive control group was treated the same as the sample group. Three parallels were set up for each group.

[0059]

[0060] Immediately use the microplate reader to detect, set the parameters: slow oscillation for 10 seconds, OD410nm absorbance measurement. Export the file after the instrument completes the reading.

[0061] 3.2 Result calculation Calculate the inhibition rate of elastase activity:

[0062] Where: T—absorbance value of the sample group, that is, the absorbance value of the solution after the sample affects the reaction between elastase and substrate AAAPAN.

[0063] To—absorbance value of sample background group.

[0064] C—Absorbance value of enzyme reaction group, that is, the absorbance value of the reaction between elastase and substrate AAAPAN when no sample is added.

[0065] Co—Solvent background group absorbance value.

[0066] 3.3 Results The experimental data are expressed as mean±SD, and statistical methods such as t-test can be used to analyze the data.

[0067] 3. Experimental Results Reference Figure 1 : Schematic diagram of the inhibition rate of the test substances on elastase activity.

[0068] The results of the inhibition rate of the test substance on elastase activity are as follows:

[0069] Compared with the blank group: *, p<0.05; **, p<0.01; ***, p<0.001, ****, p<0.0001.

[0070] Test conclusion: 100% (V / V) of the test substance inhibited the decomposition of AAAPAN catalyzed by elastase, with an inhibition rate of 20.58±6.89%, and the data had a significant difference (p=0.0454). This test result can be used as one of the evidences to support that the test substance achieves firming and anti-wrinkle effects by inhibiting the activity of elastase.

[0071] Skin Conditioning Test: Sample 1#: Humectant: Glycerin 3%.

[0072] Antioxidant: p-Hydroxyacetophenone 0.1%.

[0073] Skin Conditioners Skin conditioning agent a: Palmitoyl pentapeptide-4 0.1%.

[0074] Sample 2#: Humectant: Glycerin 3%.

[0075] Antioxidant: p-Hydroxyacetophenone 0.1%.

[0076] Skin Conditioners Skin conditioning agent b: Acetyl hexapeptide-1 0.1% Sample 3#: Humectant: Glycerin 3%.

[0077] Antioxidant: p-Hydroxyacetophenone 0.1%.

[0078] Skin Conditioners Skin conditioning agent a: Palmitoyl pentapeptide-4 0.1%.

[0079] Skin conditioning agent b: Acetyl hexapeptide-1 0.1% Skin conditioning agent c: Acetyl hexapeptide-8 0.01%, dipeptide diaminobutyroyl benzylamide diacetate 0.01%, carnosine 0.02%, glutathione 0.01%, oligopeptide-1 0.01%, dipeptide-2 0.01%, tripeptide-1 copper 0.01%, acetyl dipeptide-1 cetyl ester 0.01%, tripeptide-32 0.01%.

[0080] The above three groups of samples were experimentally compared and feedback was given on human skin. The test showed that sample 3# was superior to sample 1# and sample 2# in terms of firmness, skin feel and comfort.

[0081] A composite polypeptide firming essence without antioxidants is made of the following raw materials in percentage by weight: 3%-5% moisturizer, 0.3%-5% skin conditioner, and the rest is purified water, wherein the skin conditioner is a mixture of skin conditioner a, skin conditioner b and skin conditioner c in any proportion. The skin conditioner a is a mixture of one or more of dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7, and acetyl tetrapeptide-5 in any proportion. The skin conditioner b is a mixture of one or more of acetyl hexapeptide-8, acetyl hexapeptide-1, and acetyl hexapeptide-3 in any proportion; the skin conditioner c is an anti-aging composite 9-peptide composition.

[0082] The anti-aging composite 9-peptide composition is made of the following raw materials in weight percentage: acetyl hexapeptide-8 10%, dipeptide diaminobutyryl benzylamide diacetate 10%, carnosine 20%, glutathione 10%, oligopeptide-1 10%, dipeptide-2 10%, tripeptide-1 copper 10%, acetyl dipeptide-1 cetyl ester 10%, tripeptide-32 10%. The moisturizer is one or a mixture of any proportion of two or more of glycerol, propylene glycol, sodium hyaluronate, and 1,2-hexanediol. Four specific implementations are given below.

[0083] Example 5: Except for the absence of antioxidant, the other components and weights are the same as those of Example 1.

[0084] Example 6: Except for the absence of antioxidant, the other components and weights are the same as those of Example 2.

[0085] Example 7: Except for the absence of antioxidant, the other components and weights are the same as those of Example 3.

[0086] Example 8: Except for the absence of antioxidant, the other components and weights are the same as those of Example 4.

[0087] The steps of preparing the composite polypeptide firming essence without antioxidants are as follows: (1) Add the above amount of moisturizer and purified water into an emulsifying pot, heat to 85°C, and stir for 15 minutes until completely dissolved to obtain phase A. There is no strict requirement for the stirring speed, and it can be operated according to the instructions of the equipment.

[0088] (2) Cool down to 35-45℃ (no strict requirement, this range can be satisfied), filter and sterilize, take samples for inspection, and obtain phase B after passing the micro-inspection. There is no strict requirement for filter sterilization, and the operation can be carried out in accordance with the basic or relevant standards of the industry.

[0089] (3) Take an appropriate amount of phase B (the appropriate amount here means that the skin conditioner can be completely dissolved, and the weight of phase B is generally 3-5 times that of phase C), mix phase B and the skin conditioner in a reactor, and then filter to obtain phase C.

[0090] (4) Add phase C to the remaining phase B, stir and mix evenly, and then aseptically package to obtain a complex polypeptide firming essence without antioxidants.

[0091] In the above two preparation methods, unless otherwise specified, they are carried out at room temperature and pressure in an environment that meets the requirements for cosmetic production.

[0092] The products of Examples 5-8 were tested and the results were similar to those of Examples 1-4.

[0093] The skin conditioner in the formula of the present invention is a combined formula, in particular, an anti-aging composite 9-peptide composition is used, and a plurality of firming and anti-wrinkle composite polypeptides are effectively combined to promote the penetration of the composite polypeptides from multiple directions and multiple targets, thereby endowing the skin with multi-level cell reconstruction and regeneration, enhancing skin integrity, increasing skin firmness and elasticity, effectively resisting skin aging, and achieving diversified long-term moisturizing and firming of the skin. There is no need to add thickeners and preservatives to the product (especially preservatives that affect the activity of active polypeptides), so that the product has the advantages of easy absorption, no side effects, and high activity. The skin cell function is regulated through multiple targets to keep the skin in a healthy, vibrant and youthful state.

[0094] By controlling the preparation method, the present invention can choose to add antioxidants to the raw materials or remove antioxidant components, and the obtained product performance is almost the same.

[0095] The above implementation modes cannot limit the protection scope of the present invention. The equivalent modifications and changes made by those skilled in the art without departing from the overall concept of the present invention are still within the scope of the present invention.

Claims

1. A composite polypeptide firming essence containing antioxidants, characterized in that It is made of the following raw materials in percentage by weight: 3%-5% moisturizer, 0.1%-1% antioxidant, 0.3%-5% skin conditioner, and the rest is purified water; Wherein, the skin conditioning agent is a mixture of skin conditioning agent a, skin conditioning agent b and skin conditioning agent c in any proportion; The skin conditioning agent a is one or a mixture of any proportion of two or more of dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7, and acetyl tetrapeptide-5; The skin conditioning agent b is one or a mixture of any proportion of acetyl hexapeptide-8, acetyl hexapeptide-1, and acetyl hexapeptide-3; The skin conditioning agent C is an anti-aging composite 9-peptide composition.

2. The antioxidant-containing composite polypeptide firming essence according to claim 1, characterized in that The anti-aging composite 9-peptide composition is made of the following raw materials in weight percentage: 10% acetyl hexapeptide-8, 10% dipeptide diaminobutyryl benzylamide diacetate, 20% carnosine, 10% glutathione, 10% oligopeptide-1, 10% dipeptide-2, 10% tripeptide-1 copper, 10% acetyl dipeptide-1 cetyl ester, and 10% tripeptide-32.

3. The antioxidant-containing composite polypeptide firming essence according to claim 1, characterized in that The moisturizing agent is one of glycerol, propylene glycol, sodium hyaluronate, and 1,2-hexanediol, or a mixture of two or more of them in any proportion.

4. The antioxidant-containing composite polypeptide firming essence according to claim 1, characterized in that The antioxidant is p-hydroxyacetophenone.

5. A method for preparing the antioxidant-containing composite polypeptide firming essence according to any one of claims 1 to 4, characterized in that The preparation steps are as follows: (1) Add the above amount of moisturizer and purified water into an emulsifier, emulsify and homogenize for 1-2 minutes, then stir and heat to 85°C, keep warm and stir for 20 minutes until completely dissolved; (2) Add the above amount of antioxidant and stir for 5-10 minutes until completely dissolved; (3) Cool down to 45°C, add the above amount of skin conditioner, and stir for 5-10 minutes until completely dissolved; (4) After cooling to room temperature, take a sample for inspection. After passing the micro-inspection, take out the material and package it to obtain a complex polypeptide firming essence containing antioxidants.

6. A composite polypeptide firming essence without antioxidants, characterized in that Made of the following raw materials in percentage by weight: 3%-5% moisturizer, 0.3%-5% skin conditioner, and the rest is purified water; Wherein, the skin conditioning agent is a mixture of skin conditioning agent a, skin conditioning agent b and skin conditioning agent c in any proportion; The skin conditioning agent a is one or a mixture of any proportion of two or more of dipeptide diaminobutyryl benzylamide diacetate, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl tripeptide-8, acetyl tetrapeptide-5, palmitoyl tetrapeptide-7, and acetyl tetrapeptide-5; The skin conditioning agent b is one or a mixture of any proportion of acetyl hexapeptide-8, acetyl hexapeptide-1, and acetyl hexapeptide-3; The skin conditioning agent C is an anti-aging composite 9-peptide composition.

7. The antioxidant-free composite polypeptide firming essence according to claim 6, characterized in that The anti-aging composite 9-peptide composition is made of the following raw materials in weight percentage: 10% acetyl hexapeptide-8, 10% dipeptide diaminobutyryl benzylamide diacetate, 20% carnosine, 10% glutathione, 10% oligopeptide-1, 10% dipeptide-2, 10% tripeptide-1 copper, 10% acetyl dipeptide-1 cetyl ester, and 10% tripeptide-32.

8. The antioxidant-free composite polypeptide firming essence according to claim 6, characterized in that The moisturizing agent is one of glycerol, propylene glycol, sodium hyaluronate, and 1,2-hexanediol, or a mixture of two or more of them in any proportion.

9. A method for preparing the antioxidant-free composite polypeptide firming essence according to any one of claims 6 to 8, characterized in that The preparation steps are as follows: (1) Add the above-mentioned amount of moisturizer and purified water into an emulsifying pot, heat to 85°C, and stir for 15 minutes until completely dissolved to obtain phase A; (2) Cool down to 35-45°C, filter and sterilize, then take samples for inspection. After passing the micro-inspection, phase B is obtained; (3) Take an appropriate amount of phase B, mix phase B with the skin conditioning agent in a reaction kettle, and then filter to obtain phase C; (4) Add phase C to the remaining phase B, stir and mix evenly, and then aseptically package to obtain a complex polypeptide firming essence without antioxidants.

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