Composition with effects of resisting obesity, reducing blood fat and building muscles and preparation method thereof

Abstract

The invention belongs to the technical field of medicines, and discloses a composition with the effects of resisting obesity, reducing blood fat and building muscles as well as a preparation method and application of the composition. The composition provided by the invention comprises the following components: bupleurum smithii, tobacco aureocauda, physochlaina, ganoderma lucidum sporocarp, ganoderma lucidum spore powder and ganoderma lucidum spore powder wall shells. The ganoderma lucidum spore powder wall shell is innovatively used as one of the components of the composition, and full utilization of traditional Chinese medicine waste is achieved. The composition has a synergistic effect, can significantly reduce the weight, blood fat (triglyceride and total cholesterol) level and fat (epididymal fat and groin fat) weight of obese mice, and increases the total weight of muscles (flatfish muscle, gastrocnemius muscle, tibia anterior muscle, biceps brachii muscle and triceps brachii muscle).

Description

Technical Field

[0001] The present invention belongs to the field of medical technology, and in particular relates to a composition with anti-obesity, blood lipid-lowering and muscle-building effects, and a preparation method and application thereof. Background Art

[0002] Changes in lifestyle and dietary structure have become the main driving factors for the occurrence and development of cardiovascular diseases such as coronary heart disease, atherosclerosis, and hypertension. Normally, hyperlipidemia is the pathological basis for cardiovascular diseases, which is manifested as increased levels of plasma (serum) total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), and decreased levels of high-density lipoprotein cholesterol (HDL-C). Overweight and obesity are usually the external manifestations of hyperlipidemia. Studies have shown that strengthening muscles is beneficial to the prevention of cardiovascular and cerebrovascular diseases and reducing the mortality rate related to cardiovascular and cerebrovascular diseases. Therefore, intervening in key indicators such as TC, TG, and muscle production is an effective measure to prevent and treat cardiovascular and cerebrovascular diseases, including hyperlipidemia.

[0003] In recent years, with the vigorous development of traditional Chinese medicine, great progress has been made in the exploration of the efficacy of traditional Chinese medicine. Studies have shown that compared with simple Western medicine, traditional Chinese medicine has the characteristics of multiple components, multiple targets, multiple functions, treating both the symptoms and the root causes, and few toxic and side effects, and can play a synergistic role through reasonable compatibility. However, the effects of traditional Chinese medicine are complex, and unreasonable compatibility not only fails to play a synergistic role, but may even produce toxic reactions.

[0004] For example, Chinese patent CN112057546A discloses a propolis and ganoderma spore powder composition and its preparation method and application. The composition is made of the following raw materials in parts by weight: 10-20 parts of propolis, 10-20 parts of ganoderma spore powder, and 15-25 parts of traditional Chinese medicine composition. The propolis and ganoderma spore powder composition described in the invention can improve human immunity and effectively reduce blood lipids; by using propolis, ganoderma spore powder, and traditional Chinese medicine composition in combination, they can enhance each other and significantly improve human immunity and reduce blood lipids. However, the traditional Chinese medicine composition in the invention has complex ingredients, and its effects on fighting obesity and muscle gain are unknown.

[0005] Another example is Chinese patent CN116850243A, which discloses a drug preparation for treating fat deposition and obesity. The drug preparation is composed of black wolfberry, rhubarb, angelica, hawthorn, safflower, salvia miltiorrhiza, ganoderma lucidum spore powder, atractylodes, tuckahoe, coix seed, oriental rhizome, lily, tangerine peel, citron, polygonum multiflorum, senna leaf, liquorice, bupleurum, wormwood, motherwort, tangerine peel, cassia, white grass root, gynostemma pentaphyllum, dandelion, radix rehmanniae, plantain, gardenia, etc. The drug is prepared into capsules or pills. The drug preparation has high clinical efficacy, quick effect, low rebound rate, is safe and reliable, and has no obvious adverse reactions. However, the invention also has the problem of complex ingredients and uncertain effects on lowering blood lipids and increasing muscle.

[0006] Therefore, screening a Chinese medicine composition that is effective against obesity, hyperlipidemia, and muscle loss, and achieving a synergistic effect of "1+1>2" through multi-target and multi-component combinations is a technical bottleneck that urgently needs to be solved in the development and utilization of Chinese medicine. Summary of the invention

[0007] Based on the problems existing in the prior art, the present invention provides a traditional Chinese medicine composition containing Ganoderma lucidum fruiting bodies, Ganoderma lucidum spore powder, Ganoderma lucidum spore powder shell, Bupleurum chinense extract, Nicotiana canina extract, and Aspergillus niger extract, and a preparation method thereof, and clarifies the anti-obesity, blood lipid lowering and muscle building effects of the component through in vivo pharmacological experiments.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0009] Firstly, the present invention provides a composition with the effects of resisting obesity, lowering blood lipids and increasing muscle mass, characterized in that it comprises the following raw materials: 10-50 parts of small-leaf black bupleurum, 10-50 parts of yellow tobacco, and 20-50 parts of vesicularia scabra.

[0010] Preferably, the composition with anti-obesity, blood lipid lowering and muscle building effects comprises the following raw materials in parts by mass: 20-40 parts of small-leaf black bupleurum, 20-30 parts of yellow tobacco, and 20-50 parts of vesicularia scabra.

[0011] Preferably, the composition with anti-obesity, blood lipid lowering and muscle building effects comprises the following raw materials in parts by mass: 30-40 parts of small-leaf black bupleurum, 20-30 parts of yellow tobacco, and 30-50 parts of vesicularia scabra.

[0012] Preferably, the composition with anti-obesity, blood lipid lowering and muscle building effects comprises the following raw materials in parts by mass: 30 parts of small-leaf black bupleurum, 20 parts of yellow tobacco, and 50 parts of vesicularia scabra.

[0013] As some preferred embodiments, the composition further comprises 50-200 parts by weight of Ganoderma lucidum fruiting bodies.

[0014] Preferably, the composition further comprises 75-150 parts by weight of Ganoderma lucidum fruiting bodies.

[0015] More preferably, the composition further comprises 100-150 parts by weight of Ganoderma lucidum fruiting bodies.

[0016] Further preferably, the composition further comprises 125-150 parts by weight of Ganoderma lucidum fruiting bodies.

[0017] More preferably, the composition further comprises 150 parts of Ganoderma lucidum fruiting body by weight.

[0018] Wherein, the Ganoderma lucidum fruiting body includes red Ganoderma lucidum and / or purple Ganoderma lucidum.

[0019] Preferably, the Ganoderma lucidum fruiting body is Ganoderma lucidum fruiting body.

[0020] As other preferred embodiments, the composition further comprises 100-150 parts by weight of Ganoderma lucidum spore powder.

[0021] Preferably, the composition further comprises 120-150 parts by weight of Ganoderma lucidum spore powder.

[0022] More preferably, the composition further comprises 150 parts of Ganoderma lucidum spore powder by weight.

[0023] Wherein, the ganoderma lucidum spore powder includes wall-broken ganoderma lucidum spore powder and / or wall-removed ganoderma lucidum spore powder.

[0024] Preferably, the Ganoderma lucidum spore powder is wall-removed Ganoderma lucidum spore powder.

[0025] Further preferably, the composition with anti-obesity, lipid-lowering and muscle-building effects comprises the following raw materials in parts by mass: 50-200 parts of red ganoderma lucidum fruiting bodies, 100-150 parts of de-walled ganoderma lucidum spore powder, 10-40 parts of ganoderma lucidum spore powder wall shells, 10-50 parts of small-leaf bupleurum chinense, 10-50 parts of tobacco yellow flower, and 20-50 parts of vesicularia sphaerocephala.

[0026] Secondly, the present invention provides a method for preparing the above-mentioned anti-obesity, blood lipid lowering and muscle building efficacy composition, comprising the following steps:

[0027] (1) taking Ganoderma lucidum fruiting bodies, Bupleurum chinense, Nicotiana tabacum, and Phyllanthus truncatula, mixing, crushing, and sieving to obtain a mixture powder 1;

[0028] (2) taking Ganoderma lucidum spore powder, mixing it with the mixture powder 1 obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution;

[0029] (3) passing the concentrated solution obtained in step (2) through a macroporous resin, eluting, concentrating under reduced pressure, and drying to obtain a mixture powder 2;

[0030] (4) Taking the wall shell of the spore powder of Ganoderma lucidum and uniformly mixing it with the mixture powder 2 obtained in step (3) to obtain a composition powder with anti-obesity, blood lipid lowering and muscle building effects.

[0031] Preferably, the mesh size of the sieving in step (1) is 10-20 meshes.

[0032] Preferably, the amount of water added for the water extraction in step (2) is 5-20 times the total weight of the raw medicinal material, the water extraction temperature is 35-75° C., and the water extraction time is 1-5 h.

[0033] Preferably, in step (3), the loading flow rate of the concentrated solution through the macroporous resin is 1-3 BV / h, and the elution comprises first eluting with ethanol, discarding the ethanol eluate, then eluting with water, and collecting the water eluate.

[0034] Preferably, the volume fraction of the ethanol is 50-75%, the amount of ethanol used is 4-6 times the volume of the column, and the flow rate of ethanol elution is 1.5-2.5 BV / h.

[0035] Preferably, the amount of water used is 3-5 times the column volume, and the flow rate of water elution is 1-2 BV / h.

[0036] Preferably, the temperature of the reduced pressure concentration is 40-60°C, and the reduced pressure concentration is performed to a solid content of 60-80%. Preferably, the drying time is 48-72 hours, and the drying is freeze drying.

[0037] The present invention also provides the use of the above composition in preparing products with anti-obesity, blood lipid lowering and muscle building effects.

[0038] The dosage form of the product is powder, granule, capsule, tablet, oral solution or pill.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] (1) The Chinese medicine composition of the present invention has the advantages of easy availability of raw materials, simple operation, low cost, high safety, etc., is suitable for industrial production, and has broad application prospects.

[0041] (2) The present invention uses the spore powder wall shell of Ganoderma lucidum as one of the components of the composition, thereby achieving full utilization of traditional Chinese medicine waste.

[0042] (3) The Chinese medicine composition described in the present invention has the characteristics of synergistic effect.

[0043] (4) The Chinese medicine composition of the present invention has the effects of anti-obesity, lowering blood lipids and increasing muscle mass.

[0044] The raw material effects described in this application are:

[0045] Lingzhi: A fungus with medicinal value, often used in traditional Chinese medicine. Lingzhi is believed to have a variety of health benefits, such as enhancing immunity, anti-oxidation, and anti-tumor. Lingzhi spores are extremely tiny oval reproductive cells ejected from the gills of Lingzhi during its growth and maturity. Lingzhi spore powder has a variety of pharmacological activities, such as enhancing nonspecific immune function, protecting the liver, anti-cancer, lowering blood lipids, lowering blood pressure, treating neurasthenia, and improving pancreatic blood circulation.

[0046] Small-leafed black bupleurum: The root part of small-leafed black bupleurum has antipyretic, anti-inflammatory, analgesic, liver-protective, immune-enhancing and gastric cancer cell apoptosis-inducing effects. Its anti-inflammatory and liver-protective effects are better than those of northern bupleurum, and its immune-enhancing effect is significant. The flavonoids in the stems and leaves have a good protective effect on animal cholestatic, chemical, and immune liver damage. Its mechanism is related to the flavonoids' ability to scavenge free radicals and increase Na + , K + -ATPase and SOD activities, promote bile acid secretion, increase bile excretion, and thus reduce serum liver enzymes, bilirubin and liver tissue MDA levels.

[0047] Yellow tobacco: Yellow tobacco is a plant of the Solanaceae family and the genus Nicotiana. It has the functions of clearing away heat and detoxifying, sweating, calming, inducing vomiting, clearing phlegm, helping digestion, and opening the diaphragm and lowering qi. Yellow tobacco can be used as a raw material for the tobacco industry; the whole plant can also be used as a pesticide.

[0048] Phallus cystis: the whole plant of Phallus cystis, a plant of the Solanaceae family. It has the effect of clearing away heat and detoxifying. It is mainly used to treat carbuncle, sore throat, nasal sinusitis, ear infection, etc. It can be used in combination with other Chinese medicines to treat acute gastroenteritis. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 Effects of the compositions prepared in Comparative Examples 1-3 and Examples 1-4 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet;

[0050] Figure 2 Effects of the compositions prepared in Examples 5-10 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight in mice fed a high-fat diet;

[0051] Figure 3 The effects of Examples 9 and 11 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet;

[0052] Figure 4The effects of Examples 9, 12, and 13 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet;

[0053] Figure 5 The effects of Examples 9, 13 and 14 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet;

[0054] Figure 6 The effects of Examples 14-18 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet;

[0055] Figure 7 The effects of comparative examples 4-7 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet are shown.

[0056] Figure 8 The effects of Comparative Example 8 and Example 18 on body weight, serum TC and TG levels, epididymal fat and inguinal fat content, and total muscle weight of mice fed a high-fat diet are shown. DETAILED DESCRIPTION

[0057] The technical scheme of the present invention is clearly and completely described below with specific implementation methods. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention. The materials, reagents, etc. used, if not otherwise specified, are reagents and materials available from commercial sources.

[0058] Example 1 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0059] The steps include:

[0060] (1) Mix 50 parts of Bupleurum chinense, 10 parts of Nicotiana tabacum, and 20 parts of Phyllostachys chinensis, crush, and sieve to obtain a mixture powder; extract with water, and concentrate to obtain a concentrated solution;

[0061] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 1 having anti-obesity, blood lipid-lowering and muscle-building effects.

[0062] Example 2 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0063] The steps include:

[0064] (1) Mix 30 parts of Bupleurum chinense, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, crush them, and sieve them to obtain a mixture powder; extract with water, and concentrate them to obtain a concentrated solution;

[0065] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 2 having anti-obesity, blood lipid lowering and muscle building effects.

[0066] Example 3 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0067] The steps include:

[0068] (1) Mix 20 parts of Bupleurum chinense, 30 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, crush them, and sieve them to obtain a mixture powder; extract with water, and concentrate them to obtain a concentrated solution;

[0069] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 3 having anti-obesity, blood lipid lowering and muscle building effects.

[0070] Example 4 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0071] The steps include:

[0072] (1) taking 10 parts of Bupleurum chinense, 50 parts of Nicotiana tabacum, and 40 parts of Phyllostachys chinensis, mixing, crushing, and sieving to obtain a mixture powder; extracting with water, and concentrating to obtain a concentrated solution;

[0073] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 4 having anti-obesity, blood lipid lowering and muscle building effects.

[0074] Example 5 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0075] The steps include:

[0076] (1) 50 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis are mixed, crushed, and sieved to obtain a mixture powder; and water extraction and concentration are performed to obtain a concentrated solution.

[0077] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 5 having anti-obesity, blood lipid-lowering and muscle-building effects.

[0078] Example 6 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0079] The steps include:

[0080] (1) 75 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis are mixed, crushed, and sieved to obtain a mixture powder; and water extraction and concentration are performed to obtain a concentrated solution.

[0081] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 6 having anti-obesity, blood lipid lowering and muscle building effects.

[0082] Example 7 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0083] The steps include:

[0084] (1) taking 100 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of yellow tobacco, and 50 parts of vesicularia scabra, mixing, crushing, and sieving to obtain a mixture powder; extracting with water, and concentrating to obtain a concentrated solution;

[0085] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 7 having anti-obesity, blood lipid lowering and muscle building effects.

[0086] Example 8 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0087] The steps include:

[0088] (1) taking 125 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, mixing, crushing, and sieving to obtain a mixture powder; extracting with water, and concentrating to obtain a concentrated solution;

[0089] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 8 having anti-obesity, blood lipid lowering and muscle building effects.

[0090] Example 9 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0091] The steps include:

[0092] (1) 150 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of vesicularia scabra, are mixed, crushed, and sieved to obtain a mixture powder; water extraction is performed, and concentrated to obtain a concentrated solution;

[0093] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 9 having anti-obesity, blood lipid-lowering and muscle-building effects.

[0094] Example 10 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0095] The steps include:

[0096] (1) taking 200 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, mixing, crushing, and sieving to obtain a mixture powder; extracting with water, and concentrating to obtain a concentrated solution;

[0097] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 10 having anti-obesity, blood lipid-lowering and muscle-building effects.

[0098] Example 11 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0099] The steps include:

[0100] (1) taking 150 parts of Ganoderma lucidum fruiting bodies, 30 parts of Bupleurum chinense, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, mixing, crushing, and sieving to obtain a mixture powder; extracting with water, and concentrating to obtain a concentrated solution;

[0101] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 11 having anti-obesity, blood lipid-lowering and muscle-building effects.

[0102] Example 12 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0103] The steps include:

[0104] (1) Mix 30 parts of Bupleurum chinense, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, crush them, and sieve them to obtain a mixture powder;

[0105] (2) taking 100 parts of broken spore powder of Ganoderma lucidum, mixing it with the mixture powder obtained in step (1), extracting with water, and concentrating it to obtain a concentrated solution;

[0106] (3) The concentrated solution obtained in step (2) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a composition powder 12 having anti-obesity, blood lipid lowering and muscle building effects.

[0107] Example 13 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0108] The steps include:

[0109] (1) Mix 30 parts of Bupleurum chinense, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis, crush them, and sieve them to obtain a mixture powder;

[0110] (2) taking 100 parts of the spore powder of Ganoderma lucidum, mixing it with the mixture powder obtained in step (1), extracting with water, and concentrating it to obtain a concentrated solution;

[0111] (3) The concentrated solution obtained in step (2) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a mixture powder 13.

[0112] Example 14 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0113] The steps include:

[0114] (1) 150 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of vesicularia scabra, are mixed, crushed, and sieved to obtain a mixture powder;

[0115] (2) taking 150 parts of spore powder of Ganoderma lucidum, mixing it with the mixture powder obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution;

[0116] (3) The concentrated solution obtained in step (2) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a mixture powder 14.

[0117] Example 15 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0118] The steps include:

[0119] (1) 150 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis were mixed, crushed, and sieved to obtain a mixture powder 1;

[0120] (2) taking 150 parts of spore powder of Ganoderma lucidum, mixing it with the mixture powder 1 obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution;

[0121] (3) passing the concentrated solution obtained in step (2) through a macroporous resin, eluting, concentrating under reduced pressure, and drying to obtain a mixture powder 2;

[0122] (4) Take 10 parts of the spore powder and shell of Ganoderma lucidum, and mix them evenly with the mixture powder obtained in step (3) to obtain a composition powder 15 with anti-obesity, blood lipid lowering and muscle building effects.

[0123] Example 16 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0124] The steps include:

[0125] (1) 150 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis were mixed, crushed, and sieved to obtain a mixture powder 1;

[0126] (2) taking 150 parts of spore powder of Ganoderma lucidum, mixing it with the mixture powder 1 obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution;

[0127] (3) passing the concentrated solution obtained in step (2) through a macroporous resin, eluting, concentrating under reduced pressure, and drying to obtain a mixture powder 2;

[0128] (4) Take 20 parts of the spore powder and shell of Ganoderma lucidum, and mix them evenly with the mixture powder 2 obtained in step (3) to obtain a composition powder 16 with anti-obesity, blood lipid lowering and muscle building effects.

[0129] Example 17 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0130] The steps include:

[0131] (1) 150 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis were mixed, crushed, and sieved to obtain a mixture powder 1;

[0132] (2) taking 150 parts of spore powder of Ganoderma lucidum, mixing it with the mixture powder 1 obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution;

[0133] (3) passing the concentrated solution obtained in step (2) through a macroporous resin, eluting, concentrating under reduced pressure, and drying to obtain a mixture powder 2;

[0134] (4) Take 30 parts of the spore powder and shell of Ganoderma lucidum, and mix them evenly with the mixture powder 2 obtained in step (3) to obtain a composition powder 17 with anti-obesity, blood lipid lowering and muscle building effects.

[0135] Example 18 A composition having anti-obesity, blood lipid lowering and muscle building effects and its preparation method

[0136] The steps include:

[0137] (1) 150 parts of red ganoderma lucidum fruiting bodies, 30 parts of small-leaved black bupleurum, 20 parts of Nicotiana tabacum, and 50 parts of Phyllostachys chinensis were mixed, crushed, and sieved to obtain a mixture powder 1;

[0138] (2) taking 150 parts of spore powder of Ganoderma lucidum, mixing it with the mixture powder 1 obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution;

[0139] (3) passing the concentrated solution obtained in step (2) through a macroporous resin, eluting, concentrating under reduced pressure, and drying to obtain a mixture powder 2;

[0140] (4) Take 40 parts of the spore powder and shell of Ganoderma lucidum, and mix them evenly with the mixture powder 2 obtained in step (3) to obtain a composition powder 18 with anti-obesity, blood lipid lowering and muscle building effects.

[0141] Comparative Example 1-5 A composition having anti-obesity, blood lipid lowering and muscle building effects and a preparation method thereof

[0142] The steps include:

[0143] (1) taking 100 parts of each of radix bupleuri, nicotiana canadensis, vesicularia sutchuenensis, red ganoderma lucidum fruiting body, and broken wall ganoderma spore powder, crushing and sieving; extracting with water, and concentrating to obtain red ganoderma lucidum fruiting body concentrate, broken wall ganoderma spore powder concentrate, radix bupleuri concentrate, nicotiana canadensis concentrate, and vesicularia sutchuenensis concentrate;

[0144] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain sample powders of red ganoderma lucidum fruiting bodies, wall-removed ganoderma lucidum spore powder, small-leaved black bupleurum, yellow flowered tobacco, and vesicularia sphaerocephala.

[0145] Bupleurum chinense Nicotiana tabacum Vesicularia Ganoderma lucidum fruiting body Broken wall Ganoderma lucidum spore powder Comparative Example 1 100 0 0 0 0 Comparative Example 2 0 100 0 0 0 Comparative Example 3 0 0 100 0 0 Comparative Example 4 0 0 0 100 0 Comparative Example 5 0 0 0 0 100

[0146] Comparative Example 6 A composition having anti-obesity, blood lipid lowering and muscle building effects and a preparation method thereof

[0147] The steps include:

[0148] (1) taking 100 parts of red ganoderma lucidum fruiting bodies, crushing and sieving; extracting with water and concentrating to obtain red ganoderma lucidum fruiting body concentrate;

[0149] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a red ganoderma lucidum fruiting body sample powder.

[0150] (3) Take 10 parts of the spore powder and shell of Ganoderma lucidum, and mix them evenly with the red Ganoderma lucidum fruiting body sample powder obtained in step (2) to obtain a composition powder 19 with anti-obesity, lipid-lowering and muscle-building effects.

[0151] Comparative Example 7 A composition having anti-obesity, blood lipid lowering and muscle building effects and a preparation method thereof

[0152] The steps include:

[0153] (1) taking 100 parts of broken-wall Ganoderma lucidum spore powder, crushing, sieving; extracting with water, and concentrating to obtain a broken-wall Ganoderma lucidum spore powder concentrate;

[0154] (2) The concentrated solution obtained in step (1) is passed through a macroporous resin, eluted, concentrated under reduced pressure, and dried to obtain a broken-wall Ganoderma lucidum spore powder sample powder.

[0155] (3) Taking 10 parts of the wall shell of the Ganoderma lucidum spore powder, and uniformly mixing it with the powder of the broken wall Ganoderma lucidum spore powder sample obtained in step (2), to obtain a composition powder 20 with anti-obesity, blood lipid lowering and muscle building effects.

[0156] Comparative Example 8 A composition was prepared according to the components and preparation method described in Example 4 in the comparative document CN112057546A. The specific preparation method is:

[0157] (1) Add 20 parts of Ophiopogon japonicus, 20 parts of Eclipta prostrata, 18 parts of Astragalus membranaceus, 15 parts of Bupleurum chinense, 15 parts of Cassia seed, 12 parts of Paeonia lactiflora, 10 parts of Glycyrrhiza uralensis, 10 parts of Lycopodiella ciliata, 10 parts of Schizonepeta tenuifolia, and 8 parts of Angelica sinensis to 1104 parts of deionized water and soak for 0.5-2 h, heat and reflux for extraction 3 times, each time for 1 h, combine the filtrates, concentrate under reduced pressure to , vacuum dry, and crush to 80 mesh to obtain a Chinese medicine extract.

[0158] (2) 12 parts of propolis, 18 parts of broken wall Ganoderma lucidum spore powder, and 20 parts of the traditional Chinese medicine composition are mixed evenly to obtain a composition powder 21.

[0159] Effect Experiment

[0160] 1. Animal Experiments

[0161] (1) Experimental Materials

[0162] Mice: Male C57BL / 6 mice were purchased from the Experimental Animal Center of Zhejiang Academy of Medical Sciences, animal license: SYXK2018-0011, weighing 18-22 g, and were raised in the SPF-grade experimental animal environment of Zhejiang Institute of Traditional Chinese Medicine Co., Ltd. The optimal temperature was 20-26 °C, the relative humidity was 50%-60%, and the mice were free to eat.

[0163] Sample: The sample powders prepared in Examples 1-18 and Comparative Examples 1-8 were prepared into 10 mg / mL sample solutions with sterile water for later use.

[0164] (3) Experimental methods

[0165] Grouping: C57BL / 6 mice were adaptively raised for 7 days and fed with a normal diet (4% fat, 20% carbohydrates and 20% protein). The mice were randomly divided into a control group, a model group and a sample group according to their body weight, with 10 mice in each group.

[0166] Feed replacement: The mice in the control group continued to eat ordinary feed, while the mice in the model group and sample group were replaced with a high-fat feed (60% fat, 20% carbohydrates and 20% protein).

[0167] Sample administration: The sample group was given sample solution by oral gavage at a dose of 400 mg / kg, and the sample was given by oral gavage for 8 weeks, once a day. The control group and the model group were given an equal amount of sterile water, once a day. The mouse feed was withdrawn 24 hours after the last oral gavage treatment, and the mice were fasted for 16 hours. The mice were weighed and then killed; blood was collected from the abdominal aorta, and the serum was separated to detect the concentration of TG and TC; the soleus muscle, gastrocnemius muscle, tibialis anterior muscle, biceps brachii, and triceps brachii were stripped, and the total muscle weight was weighed.

[0168] (4) Experimental results

[0169] Depend on Figure 1 The results show that compared with the control group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the model group mice were significantly increased, while the total muscle weight was significantly decreased; compared with the model group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the mice in each combination powder sample group were all reduced, and the effect of combination powder 2 was the best. However, the total muscle weight of each sample group did not increase significantly.

[0170] Depend on Figure 2 The results show that compared with the control group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the model group mice were significantly increased, while the total muscle weight was significantly decreased; compared with the model group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the combination powder group 2 mice were all reduced, but the total muscle weight did not increase significantly. Compared with the combination powder group 2 mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the combination powder 5-10 groups of mice were further reduced, while the total muscle weight was significantly increased, and compared with the combination powder 9, the effect of the combination powder 10 was not significantly improved.

[0171] Depend on Figure 3 From the results, it can be seen that compared with the control group mice, the body weight, serum TC and TG contents, epididymal fat and inguinal fat weights of the model group mice were significantly increased, while the total muscle weight was significantly decreased; compared with the model group mice, the body weight, serum TC and TG contents, epididymal fat and inguinal fat weights of the composition powder 9 and 11 groups of mice were reduced, while the total muscle weight was increased, and the effect of composition powder 9 was better than that of composition powder 11, indicating that the activity of the composition containing Ganoderma lucidum is better than that of the composition containing Ganoderma lucidum.

[0172] Depend on Figure 4It can be seen from the results that compared with the control group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the model group mice were significantly increased, while the total muscle weight was significantly decreased; compared with the model group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the combination powder sample 9, 12 and 13 groups of mice were all reduced, while the total muscle weight was increased, and the effect of the combination powder sample 13 was the best, indicating that the activity of the composition containing de-walled Ganoderma lucidum spore powder is better than the composition containing broken wall Ganoderma lucidum spore powder or Ganoderma lucidum fruiting body.

[0173] Depend on Figure 5 It can be seen from the results that compared with the control group mice, the body weight, serum TC and TG contents, epididymal fat and inguinal fat weights of the model group mice were significantly increased, while the total muscle weight was significantly decreased; compared with the model group mice, the body weight, serum TC and TG contents, epididymal fat and inguinal fat weights of the combination powder sample 9, 13 and 14 groups of mice were all reduced, while the total muscle weight was increased, and the effect of the combination powder sample 14 was the best, indicating that the lipid-lowering effect and muscle-increasing effect of the wall-removed Ganoderma lucidum spore powder and Ganoderma lucidum fruiting body have synergistic effects.

[0174] Depend on Figure 6 It can be seen from the results that compared with the control group mice, the body weight, serum TC and TG contents, epididymal fat and inguinal fat weights of the model group mice were significantly increased, while the total muscle weight was significantly decreased; compared with the model group mice, the body weight, serum TC and TG contents, epididymal fat and inguinal fat weights of the combination powder sample 14-18 groups of mice were all reduced, while the total muscle weight was increased, and the effect of combination powder 16 was the best.

[0175] Depend on Figure 7 The results show that compared with the control group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the model group mice were significantly increased, while the total muscle weight was significantly reduced; compared with the model group mice, the body weight, serum TC and TG content, epididymal fat and inguinal fat weight of the mice in each combination powder sample group were reduced, and the effect of combination powder 19 and combination powder 20 was the best, indicating that the wall shell of Ganoderma lucidum spore powder has a promoting effect on the efficacy of Ganoderma lucidum fruiting body and broken wall Ganoderma lucidum spore powder. In addition, combination powder 19 can significantly increase the total muscle weight.

[0176] Depend on Figure 8 It can be seen from the results that the effect of combination powder 21 on reducing the body weight, blood lipid level and fat weight of obese mice is significantly inferior to combination powder 18, and the effect on increasing the total muscle weight is also not significant, which is significantly different from combination powder 18.

[0177] The above results indicate that the Chinese medicine composition with anti-obesity, blood lipid lowering and muscle building effects formulated in a specific proportion of the present invention can significantly reduce the body weight, blood lipid (triglyceride and total cholesterol) levels and fat (epididymal fat, inguinal fat) weight of obese mice, and increase the total weight of muscles (soleus, gastrocnemius, tibialis anterior, biceps, triceps).

[0178] The above description is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several modifications or equivalent substitutions can be made to the technical solution without departing from the principle of the present invention. These modifications or equivalent substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A composition having anti-obesity, blood lipid lowering and muscle building effects, characterized in that: The invention comprises the following components by weight: 10-50 parts of small-leaf black bupleurum, 10-50 parts of yellow tobacco and 20-50 parts of vesicularia scabra.

2. The composition according to claim 1, characterized in that: The composition further comprises 50-200 parts by weight of ganoderma lucidum fruiting bodies, wherein the ganoderma lucidum fruiting bodies include red ganoderma and / or purple ganoderma.

3. The composition according to claim 1, characterized in that: The composition further comprises 100-150 parts of ganoderma lucidum spore powder by weight, wherein the ganoderma lucidum spore powder comprises wall-broken ganoderma lucidum spore powder and / or wall-removed ganoderma lucidum spore powder.

4. The composition according to claim 1, characterized in that: The composition further comprises 10-40 parts by weight of ganoderma lucidum spore powder wall shell.

5. The composition according to any one of claims 1 to 4, characterized in that: The raw materials include the following by weight: 50-200 parts of red ganoderma lucidum fruiting bodies, 100-150 parts of wall-removed ganoderma lucidum spore powder, 10-40 parts of ganoderma lucidum spore powder wall shells, 10-50 parts of small-leaf black bupleurum, 10-50 parts of yellow tobacco, and 20-50 parts of vesicular herb.

6. A method for preparing the composition according to any one of claims 1 to 5, characterized in that: The steps include: (1) taking Ganoderma lucidum fruiting bodies, Bupleurum chinense, Nicotiana tabacum, and Phyllanthus truncatula, mixing, crushing, and sieving to obtain a mixture powder 1; (2) taking Ganoderma lucidum spore powder, mixing it with the mixture powder 1 obtained in step (1), extracting with water, and concentrating to obtain a concentrated solution; (3) passing the concentrated solution obtained in step (2) through a macroporous resin, eluting, concentrating under reduced pressure, and drying to obtain a mixture powder 2; (4) Taking the spore powder wall shell of Ganoderma lucidum and uniformly mixing it with the mixture powder 2 obtained in step (3) to obtain the composition.

7. The preparation method according to claim 6, characterized in that: The mesh size of the sieving in step (1) is 10-20 mesh; The water extraction temperature in step (2) is 35-75° C., the water extraction time is 1-5 h, and the amount of water added for water extraction is 5-20 times the total weight of the raw medicinal materials; The concentrated solution in step (3) is loaded onto the macroporous resin at a flow rate of 1-3 BV / h; the elution comprises first eluting with ethanol, then discarding the ethanol eluate, then eluting with water, and collecting the eluate; The temperature of the reduced pressure concentration in step (3) is 40-60°C; the reduced pressure concentration is performed to a solid content of 60-80%; the drying time is 48-72h; and the drying is freeze drying.

8. The preparation method according to claim 6, characterized in that: The volume fraction of ethanol in step (3) is 50-75%, the amount of ethanol used is 4-6 times the column volume, and the flow rate of ethanol elution is 1.5-2.5BV / h; the flow rate of water elution is 1-2BV / h, and the amount of water used is 3-5 times the column volume.

9. Use of the composition according to any one of claims 1 to 5 in preparing a product having anti-obesity, blood lipid lowering and muscle building effects.

10. A product with anti-obesity, blood lipid lowering and muscle building effects, characterized in that: The composition comprises the composition according to any one of claims 1 to 5, wherein the dosage form of the product is powder, granule, capsule, tablet, oral solution or pill.

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