Composition capable of benefiting qi, nourishing blood, maintaining beauty and keeping young as well as preparation method and application of composition

Abstract

The invention discloses a composition for benefiting qi, nourishing blood, maintaining beauty and keeping young as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The composition disclosed by the invention comprises donkey-hide gelatin, longan aril, hawthorn, Chinese yam, astragalus membranaceus, rose, mulberry, codonopsis pilosula, poria cocos, raspberry, liquorice, Chinese date, red ginseng and ginger. The composition disclosed by the invention is proper in compatibility, the raw medicinal materials are comprehensive in effect, experiments prove that the composition has various effects of benefiting qi and nourishing blood, invigorating spleen and tonifying lung, calming heart and relieving restlessness, nourishing yin and moistening dryness and the like, is suitable for various diseases such as deficiency of qi and blood, oxidative aging and the like caused by deficiency of qi and blood, and has the curative effect equivalent to that of compound donkey-hide gelatin pulp in the aspects of benefiting qi and nourishing blood; in the aspect of maintaining beauty and keeping young, the curative effect is equivalent to that of eight-component kidney-tonifying capsules.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and specifically relates to a composition for invigorating qi and blood, beautifying and nourishing the skin, and a preparation method and application thereof. Background Art

[0002] Qi and blood deficiency is the name of the syndrome, also known as qi and blood deficiency, which refers to the syndrome of insufficient qi and blood. It is mostly caused by long-term illness and damage to qi and blood. Blood deficiency can be caused by qi deficiency and inability to produce blood, and qi deficiency can also be caused by blood deficiency. Clinical manifestations Qi deficiency and blood deficiency exist at the same time, with symptoms such as shortness of breath, laziness, fatigue, spontaneous sweating, dizziness, palpitations and insomnia, pale or sallow complexion, etc. As people's life pressure increases and the pace of life accelerates, more and more people suffer from qi and blood deficiency and premature skin aging due to various unhealthy lifestyles such as excessive fatigue, improper diet, and staying up late. If qi and blood are not replenished in time, people will be tired, sallow, and have low immunity, which will affect people's normal life. At present, the clinical treatment methods of invigorating the spleen and stomach, replenishing qi and producing blood, replenishing the kidney and producing blood, removing blood stasis and producing blood, and detoxifying and producing blood are mostly used.

[0003] Compound donkey-hide gelatin syrup generally has the effects of replenishing qi and blood, moistening the lungs and relieving cough, nourishing yin and tonifying the kidneys, etc. It can be used to improve qi and blood deficiency, dry lung cough, insomnia, dreaminess, irritability, etc. Qi-replenishing and blood-nourishing compound donkey-hide gelatin syrup is a Chinese patent medicine, which contains Chinese medicinal ingredients such as donkey-hide gelatin, red dates, longan pulp, donkey-hide gelatin, hawthorn, etc. It has the effect of replenishing qi and blood, and can be used to improve fatigue, pale complexion, dizziness, palpitations, insomnia, etc. caused by qi and blood deficiency, but the effect of delaying skin aging is not obvious.

[0004] Ba Zi Bushen Capsule is based on Wu Zi Yan Zong Wan, a famous doctor in Ming Dynasty, Zhang Shiche, but without Plantago seed, and with Cnidium monnieri, Allium chinense seed, Deer antler and seahorse. It can nourish kidney essence and warm kidney yang at the same time. It can replenish the innate essence and strengthen the innate ability. It is an excellent product for nourishing kidney essence. Because kidney is the innate foundation, it is responsible for bone and marrow production, storage of essence and qi, and reproduction and growth. It is the fundamental driving force of all life activities of the human body. Without kidney essence as a prerequisite, all life activities will no longer exist. The main manifestation of insufficient kidney essence is premature aging. Therefore, traditional Chinese medicine believes that replenishing kidney essence is an effective way to resist aging, premature aging, and beauty. Many experiments and clinical studies have confirmed that Ba Zi Bushen Capsule has the unique advantages of preventing and treating overall aging, systemic aging and aging-related diseases, and is expected to become a representative Chinese patent medicine for anti-aging in traditional Chinese medicine, but the effect of replenishing qi and nourishing blood is not obvious.

[0005] In view of this, in order to solve the deficiencies of the existing Chinese patent medicine technology, the present invention provides a composition for replenishing qi and nourishing blood, beautifying and nourishing the skin, and its preparation method and application. The composition disclosed in the present invention can not only replenish qi and nourish blood, but also beautify and nourish the skin, and has a good research and development prospect. Summary of the invention

[0006] Terms and Claims of the Present Invention:

[0007] 1. As used herein, the articles "a", "an" and "the" include plural referents unless expressly limited to one or more referents otherwise.

[0008] 2. As used herein, numerical range: Unless otherwise expressly indicated, all ranges or ratios disclosed herein will be understood to include any and all sub-ranges or sub-ratios contained therein. For example, a range or ratio of 1 to 30 stated should be considered to be included between a minimum value of 1 and a maximum value of 30, and any sub-range or sub-ratio, integer, decimal, or sub-range or sub-ratio consisting of integers or decimals including the end points.

[0009] 3. As used herein, the terms "comprises," "including," "have," "have," "may," "contain" and variations thereof mean open-ended conjunctions or terms that do not exclude the possibility of additional compositions or structures.

[0010] Ejiao is a solid animal glue that is concentrated from boiled donkey skin.

[0011] Longan pulp is the aril of longan, a plant of the Sapindaceae family.

[0012] Hawthorn is the dried mature fruit of Crataegus pinnatifida Bge.var.major NEBr. or Crataegus pinnatifida Bge., a plant of the Rosaceae family.

[0013] Yam is the dried rhizome of Dioscorea opposita Thunb., a plant of the Dioscoreaceae family.

[0014] Astragalus is the dried root of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge. of the Leguminosae family.

[0015] Rose is the dried flower buds of Rosa rugosa Thunb., a plant of the Rosaceae family.

[0016] Mulberry is the dried fruit cluster of Morus alba L., a plant of the Moraceae family.

[0017] Codonopsis pilosula is the dried root of Codonopsis pilosula (Franch.) Nannf., Codonopsis pilosula Nannf.var.modesta (Nannf.) LTShen or Codonopsis tangshen Oliv. of the Campanulaceae family.

[0018] Poria cocos is the dried sclerotium of the Polyporaceae fungus Poria cocos (Schw.) Wolf.

[0019] Raspberry is the dried fruit of Rubus chingii Hu, a plant of the genus Rubus in the Rosaceae family.

[0020] Licorice is the dried root of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the Leguminosae family.

[0021] Jujube is the dried mature fruit of the jujube plant of the Rhamnaceae family.

[0022] Red ginseng is the steamed dried root of the cultivated product of Panax ginseng CAMey. (commonly known as round ginseng), a plant of the Araliaceae family.

[0023] Ginger is the fresh rhizome of the ginger plant of the ginger family.

[0024] Compound donkey-hide gelatin slurry was purchased from Dong-E-E-Jiao Co., Ltd., approval number: National Medicine Standard Z37021371; specification: 20 mL / tube.

[0025] Bazi Bushen Capsules were purchased from Shijiazhuang Yiling Pharmaceutical Co., Ltd., approval number: National Medicine Standard B20020585, specification: 0.4 grams per capsule.

[0026] In view of this, the purpose of the present invention is to provide a composition for replenishing qi and nourishing blood, and beautifying and nourishing the skin, as well as a preparation method and application thereof. Compared with the existing Chinese patent medicine technology, the composition disclosed in the present invention has dual functions, which can not only replenish qi and nourish blood, but also beautify and nourish the skin.

[0027] To achieve the above-mentioned object of the invention, the technical solution of the present invention is as follows:

[0028] On the one hand, the present invention provides a composition for replenishing qi and blood and beautifying the skin, the composition comprising: donkey-hide gelatin, longan pulp, hawthorn, yam, astragalus, rose, mulberry, codonopsis, poria, raspberry, licorice, jujube, red ginseng and ginger.

[0029] The prescription of the present invention is explained as follows: Astragalus is sweet and warm, and can nourish the spleen and replenish qi; Longan meat is sweet and flat, and can both nourish the spleen and nourish the heart and blood, and they are all monarch drugs. Codonopsis and Chinese yam are both important drugs for nourishing the spleen and replenishing qi. When combined with Astragalus, the effect of nourishing the spleen and replenishing qi is more prominent; Ejiao nourishes blood and nourishes yin, red ginseng nourishes qi and absorbs blood, and Poria strengthens the spleen and calms the mind. The three drugs are combined with longan meat, and the power of nourishing heart blood and calming the mind is stronger, and they are all minister drugs. Rose, which can promote qi and relieve depression, hawthorn, which can promote qi and digestion, mulberry, which can nourish blood and nourish yin, and raspberry, which can benefit the liver and nourish the kidney, are used to enhance the efficacy of nourishing qi and blood and make it nourish without stagnation; ginger and jujube are used to harmonize the spleen and stomach to provide the source of transformation; the above drugs are all adjuvant drugs. Roasted licorice nourishes the qi of the heart and spleen, and harmonizes the drugs, and is used as a guiding drug. When the drugs are compatible, the heart and spleen are nourished, the qi and blood are nourished, and the symptoms are eliminated.

[0030] Preferably, the composition comprises, by weight: 0.5-6 parts of donkey-hide gelatin, 0.5-9 parts of longan pulp, 2-12 parts of hawthorn, 22-55 parts of yam, 45-72 parts of astragalus, 2-12 parts of rose, 1-10 parts of mulberry, 14-32 parts of codonopsis, 27-46 parts of poria, 0.5-6 parts of raspberry, 14-28 parts of licorice, 2-12 parts of jujube, 0.5-6 parts of red ginseng and 28-42 parts of ginger.

[0031] Further preferably, the composition comprises, by weight: 1-5 parts of donkey-hide gelatin, 1-8 parts of longan pulp, 4-10 parts of hawthorn, 30-50 parts of yam, 50-70 parts of astragalus, 4-10 parts of rose, 2-8 parts of mulberry, 15-30 parts of codonopsis, 30-44 parts of poria, 1-5 parts of raspberry, 15-25 parts of licorice, 4-10 parts of jujube, 1-5 parts of red ginseng and 30-40 parts of ginger.

[0032] Finally, preferably, the composition includes, by weight: 3 parts of donkey-hide gelatin, 4 parts of longan pulp, 8 parts of hawthorn, 40 parts of yam, 60 parts of astragalus, 6 parts of rose, 4 parts of mulberry, 24 parts of codonopsis, 40 parts of poria, 3 parts of raspberry, 18 parts of licorice, 8 parts of jujube, 2 parts of red ginseng and 36 parts of ginger.

[0033] On the other hand, the present invention provides a method for preparing the above-mentioned Qi-replenishing, blood-nourishing, beautifying and skin-nourishing composition, comprising the following steps: extracting a formulated amount of medicinal materials with water to obtain an extract.

[0034] Preferably, the method comprises the following steps: adding water, heating and refluxing for extraction 2-4 times, adding 5-15 times the weight of the medicinal material of water each time, and extracting for 1-3 hours each time to obtain an extract.

[0035] More preferably, the method comprises the following steps: adding water, heating and reflux extraction twice, adding 12 times the weight of water of the medicinal material for the first time, and extracting for 80 minutes; adding 10 times the weight of water of the medicinal material for the second time, and extracting for 40 minutes to obtain an extract.

[0036] More preferably, the preparation method further comprises the following steps: concentrating the extract under reduced pressure to form a thick paste, drying under reduced pressure, and crushing into powder to obtain an extract.

[0037] More preferably, the preparation method further comprises the following steps: concentrating the extract under reduced pressure to a thick paste with a relative density of 1.2-1.3 (thermal measurement at 50-60°C), drying under reduced pressure at 60-70°C, and crushing into powder to obtain an extract.

[0038] In another aspect, the present invention provides a medicine, the active ingredient of which includes the above composition, or the extract prepared according to the above preparation method.

[0039] The drug can be prepared into dosage forms such as pills, capsules, granules, oral liquids, powders, tablets, lozenges, ointments, etc., and suitable drug carriers in the art can be selected for different dosage forms.

[0040] The pharmaceutical carrier used can be solid, liquid or gas. Examples of solid carriers include lactose, kaolin, sucrose, talc, gelatin, agar, pectin, gum arabic, magnesium stearate and stearic acid. Examples of liquid carriers include syrup, peanut oil, olive oil and water. Examples of gaseous carriers include carbon dioxide and nitrogen.

[0041] When preparing the composition for oral dosage form, any convenient pharmaceutical medium can be used. For example, water, ethanol, oil, alcohol, flavoring agent, preservative, coloring agent, etc. can be used to form oral liquid preparations, such as suspensions and solutions; while carriers, such as starch, sugars, microcrystalline cellulose, diluents, granulating agents, emulsifiers, lubricants, binders, disintegrants can be used to form oral solid preparations, such as powders, capsules and tablets. Tablets and capsules are the preferred oral dosage units using solid pharmaceutical carriers due to their ease of administration. Tablets can be coated using standard aqueous or non-aqueous techniques.

[0042] Tablets containing the composition of the present invention can be prepared by tableting or molding, and one or more auxiliary ingredients or adjuvants can be used optionally. The active ingredient can be tableted in a free-flowing form (such as powder or granules) in a suitable machine, and can be optionally mixed with a binder, lubricant, inert diluent, surfactant or dispersant to prepare a tablet. Molded tablets can be molded in a suitable machine, that is, a powdered compound mixture moistened with an inert liquid diluent. Each tablet preferably contains about 0.05 mg to about 5 g of active ingredient, and each sachet or capsule preferably contains about 0.05 mg to about 5 g of active ingredient. For example, a preparation intended for oral administration to humans may contain about 0.5 mg to about 5 g of active drug, mixed with an appropriate and convenient carrier material, which may account for about 5% to 95% of the total composition. The unit dosage form usually contains about 1 mg to about 2 g of active ingredient, usually 25 mg, 50 mg, 100 mg, 200 mg, 300 mg, 400 mg, 500 mg, 600 mg, 800 mg or 1000 mg.

[0043] The pharmaceutical composition suitable for parenteral administration of the present invention can be prepared as an aqueous solution or suspension of the active compound. Suitable surfactants such as hydroxypropylcellulose can be included. Dispersions can also be prepared in glycerol, liquid polyethylene glycol and oil mixtures thereof. In addition, preservatives can be added to prevent the harmful growth of microorganisms.

[0044] The medicine of the present invention can be in a form suitable for topical use, such as an aerosol, cream, ointment, lotion, powder or the like. In addition, the composition can be in a suitable form for transdermal administration devices. These prescriptions can be prepared using the composition of the present invention by conventional processing methods. For example, a cream or ointment with a desired consistency can be prepared by mixing a hydrophilic material and water, and about 5wt% to about 10wt% of the compound.

[0045] The medicine of the present invention can be in a form suitable for rectal administration, wherein the carrier is a solid. Preferably, the mixture is made into a unit dose suppository. Suitable carriers include cocoa butter and other materials commonly used in the art. Suppositories can be prepared by first forming a composition containing a softened or melted carrier, followed by cooling and shaping in a mold.

[0046] In addition to the above-mentioned carrier components, the above-mentioned pharmaceutical preparations may include (if applicable) one or more additional carrier components, such as diluents, buffers, flavoring agents, adhesives, surfactants, thickeners, lubricants, preservatives (including antioxidants), etc. In addition, other excipients, such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, etc., colorants and flavoring agents, etc., may be added. The preparation is made isotonic with the blood of the intended recipient. The components containing the composition of the present invention can also be prepared in the form of a powder or a concentrated solution.

[0047] Preferably, the pharmaceutical preparation is an oral pharmaceutical preparation.

[0048] Further preferably, the oral pharmaceutical preparation is selected from any one of decoction, pill, tablet, capsule, powder, granule, oral liquid and ointment.

[0049] More preferably, the oral pharmaceutical preparation is an ointment.

[0050] Finally, the present invention provides the use of the above composition in preparing products for invigorating qi and nourishing blood, and beautifying and nourishing the skin.

[0051] The beneficial effects of the present invention include:

[0052] (1) The composition of the present invention is properly formulated, and the various raw medicinal materials have comprehensive effects. Experiments have confirmed that it has multiple effects such as replenishing qi and blood, strengthening the spleen and lungs, calming the mind and relieving restlessness, nourishing yin and moistening dryness. It is suitable for a variety of diseases such as qi and blood deficiency and oxidative aging caused by insufficient qi and blood. In terms of replenishing qi and blood, its efficacy is equivalent to that of compound donkey-hide gelatin syrup; in terms of beautifying and nourishing the skin, its efficacy is equivalent to that of Bazi Bushen Capsule.

[0053] (2) Animal experiments have shown that the composition of the present invention can significantly inhibit the reduction of RBC and Hb caused by Qi and blood deficiency in mice, increase the EPO content in mouse serum, reduce the IL-2 and TNF-α content in mouse serum, and significantly prolong the rotarod time and weighted swimming time of mice. DETAILED DESCRIPTION

[0054] The specific implementation methods provided by the present invention are described in detail below in conjunction with examples.

[0055] Example 1

[0056] The recipe is as follows: 3 parts of donkey-hide gelatin, 4 parts of longan meat, 8 parts of hawthorn, 40 parts of yam, 60 parts of astragalus, 6 parts of rose, 4 parts of mulberry, 24 parts of Codonopsis pilosula, 40 parts of Poria cocos, 3 parts of raspberry, 18 parts of licorice, 8 parts of jujube, 2 parts of red ginseng, and 36 parts of ginger.

[0057] Preparation method: Take the formulated amount of medicinal materials, add pure water, heat and reflux to extract twice, add 12 times the amount for the first time, extract for 80 minutes, filter the extract, continue to extract the remaining medicinal residue, add 10 times the amount of water for the second time, extract for 40 minutes, filter the extract, combine the filtrates obtained from the two extractions, and concentrate under reduced pressure to a thick paste with a relative density of 1.2 (thermal measurement at 50-60°C), dry under reduced pressure at 60-70°C, crush into powder, and make 1000 portions.

[0058] Example 2

[0059] The recipe is as follows: 1 part of donkey-hide gelatin, 8 parts of longan meat, 4 parts of hawthorn, 50 parts of yam, 50 parts of astragalus, 10 parts of rose, 2 parts of mulberry, 30 parts of Codonopsis pilosula, 30 parts of Poria cocos, 5 parts of raspberry, 25 parts of licorice, 4 parts of jujube, 5 parts of red ginseng, and 30 parts of ginger.

[0060] The preparation method is the same as Example 1.

[0061] Example 3

[0062] The recipe is as follows: 5 parts of donkey-hide gelatin, 1 part of longan meat, 10 parts of hawthorn, 30 parts of yam, 70 parts of astragalus, 4 parts of rose, 8 parts of mulberry, 15 parts of Codonopsis pilosula, 44 parts of Poria cocos, 1 part of raspberry, 15 parts of licorice, 10 parts of jujube, 1 part of red ginseng, and 40 parts of ginger.

[0063] The preparation method is the same as Example 1.

[0064] Example 4

[0065] The recipe is as follows: 0.5 parts of donkey-hide gelatin, 9 parts of longan meat, 2 parts of hawthorn, 55 parts of yam, 45 parts of astragalus, 12 parts of rose, 1 part of mulberry, 32 parts of Codonopsis pilosula, 27 parts of Poria cocos, 6 parts of raspberry, 28 parts of licorice, 2 parts of jujube, 6 parts of red ginseng, and 28 parts of ginger.

[0066] The preparation method is the same as Example 1.

[0067] Example 5

[0068] The recipe is as follows: 6 parts of donkey-hide gelatin, 0.5 parts of longan meat, 12 parts of hawthorn, 22 parts of yam, 72 parts of astragalus, 2 parts of rose, 10 parts of mulberry, 14 parts of Codonopsis pilosula, 46 parts of Poria cocos, 0.5 parts of raspberry, 14 parts of licorice, 12 parts of jujube, 0.5 parts of red ginseng, and 42 parts of ginger.

[0069] The preparation method is the same as Example 1.

[0070] Comparative Example 1

[0071] The recipe is as follows: 40 parts of Poria cocos, 60 parts of Astragalus membranaceus, 4 parts of longan pulp, 18 parts of licorice, 36 parts of ginger, and 8 parts of jujube.

[0072] The preparation method is the same as Example 1.

[0073] Comparative Example 2

[0074] The recipe is as follows: 4 parts of longan, 4 parts of mulberry, 8 parts of red dates, 18 parts of licorice, 3 parts of raspberry, 40 parts of Poria cocos, and 40 parts of yam.

[0075] The preparation method is the same as Example 1.

[0076] Comparative Example 3

[0077] The recipe is as follows: 4 parts of mulberries, 8 parts of jujubes, 8 parts of hawthorns, 2 parts of red ginseng, 4 parts of longans, 3 parts of donkey-hide gelatin, 6 parts of roses, 40 parts of poria cocos, and 18 parts of licorice.

[0078] The preparation method is the same as Example 1.

[0079] Results

[0080] 1. Research on the efficacy of replenishing qi and nourishing blood

[0081] 1.1. Construction of Qi and Blood Deficiency Mouse Model

[0082] The model was established by "tail-cutting and bleeding of mice and intraperitoneal injection of cyclophosphamide". SPF-grade Kunming male mice, 18-22g, were adaptively fed for 3 days and randomly divided into 11 groups according to body weight, with 20 mice in each group, namely blank control group, model control group, positive control group (commercially available compound donkey-hide glue paste), low-dose group of Qi and blood composition, high-dose group of Qi and blood composition, composition group 1, composition group 2, composition group 3, composition group 4, composition group 5, composition group 6 and composition group 7. Except for the low-dose group of Qi and blood paste, the dosage of the remaining groups was 10g / kg. Each group of mice was gavaged, and the model control group and the blank control group were gavaged with an equal amount of normal saline, with a gavage volume of 20mL / kg, once a day, and continued for 10 days. On the 4th, 6th and 8th days after the first administration, the tail vein of the model group and each drug-treated group was bled at 0.25 mL / 10 g. On the 5th, 7th and 9th days, the mice in the model group and each drug-treated group were intraperitoneally injected with 40, 30 and 30 mg / kg of cyclophosphamide, and the blank control group was intraperitoneally injected with normal saline, thereby preparing an animal model of qi and blood deficiency.

[0083] One hour after the last administration of the drug group, blood was collected from the tail vein to determine the red blood cell (RBC) and hemoglobin (Hb); serum was collected and enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of serum erythropoietin (EPO), interleukin-2 (IL-2), and tumor necrosis factor-α (TNF-α).

[0084] One hour after the last drug administration in the drug-treated group, the remaining mice in each group were subjected to rotating rod and weighted swimming tests. Rotarod test: The functional coordinated movement of mice was observed by a rotating rod fatigue instrument at 16 r / min, and the time the mice stayed on the rotating rod within 3 minutes was recorded. If the mouse did not fall off during the whole process, the mouse's rotating rod residence time was 180s. Weight-bearing experiment: A certain weight (10% of body weight) was tied to the tail of each group of mice, and they were placed in a swimming tank with a smooth inner wall at a water temperature of 25-30°C to swim, with the control group and the drug-treated group alternating. A stopwatch was used to record the time taken by the mouse from the start of swimming in the water to death, which was the swimming time.

[0085] 1.2. Drug preparation and daily dosage

[0086] (1) Blank control group: normal saline, 10 g kg -1 ·d -1 .

[0087] (2) Model control group: normal saline, 10 g kg -1 ·d -1 .

[0088] (3) Compound donkey-hide gelatin slurry group (positive control): Compound donkey-hide gelatin slurry, dosage: 10 g kg -1 ·d -1 , dissolved in saline.

[0089] (4) Qi-blood composition low-dose group: the product prepared in Example 1, dosage: 5 g·kg -1 ·d -1 , dissolved in saline.

[0090] (5) Qi and blood composition high dose group: the product prepared in Example 1, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0091] (6) Composition 1: the product prepared in Example 2, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0092] (7) Composition Group 2: the product prepared in Example 3, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0093] (8) Composition group 3: the product prepared in Example 4, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0094] (9) Composition Group 4: product prepared in Example 5, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0095] (10) Composition Group 5: the product prepared in Comparative Example 1, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0096] (11) Composition group 6: product prepared in comparative example 2, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0097] (12) Composition group 7: product prepared in comparative example 3, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0098] 1.3. Dosage

[0099] All animals were given an equal amount of ordinary feed + drinking water. According to the above daily dosage, each group of drugs was made into a solution and then administered by gavage for 10 consecutive days.

[0100] 1.4. Detection indicators

[0101] (1) RBC counting method: 1 hour after the last oral administration, 20 μL of blood was collected from the tail vein of the mouse and added to a test tube containing 3.98 mL of red blood cell diluent. The diluted liquid was shaken and placed on a counting plate. The diluted liquid was allowed to stand for 2-3 minutes and the number of red blood cells was recorded.

[0102] (2) Hb content determination method: 1 hour after the last intragastric administration, 20 μL of blood was collected from the tail vein and added to a test tube containing 200 μL of hydrochloric acid. After mixing, the blood was added to the hemoglobin determination tube, and the reading was recorded.

[0103] (3) ELISA method to detect EPO, IL-2 and TNF-α content in serum:

[0104] One hour after the last administration, blood was collected from mice and centrifuged at 2000 r / min for 10 min. The serum was collected and placed in a -80°C refrigerator for later use. The levels of EPO, IL-2, and IL-1β in the serum were detected.

[0105] ① Standard curve determination: Take the mother solution of each standard according to the instructions of the kit, add the standard diluent to dilute it to 5 concentrations in turn, take 100μL of the diluted standard and add it to the coated ELISA plate;

[0106] ② Add 100 μL of the sample to be tested to the test well, cover with a sealing film, seal the plate and incubate at 37°C for 90 min;

[0107] ③ Add 100 μL of biotinylated antibody to each well, cover with sealing film, seal the plate and incubate at 37°C for 60 min;

[0108] ④ Liquid preparation: dilute the concentrated detergent with distilled water to make 1x application solution for later use;

[0109] ⑤ Washing: Remove the sealing film, discard the liquid in the wells of the ELISA plate, spin dry, fill each well with washing solution, let it stand for 30 seconds and then discard it, repeat 5 times, and pat the ELISA plate dry;

[0110] ⑥ Add 100 μL of enzyme binding working solution to each well, cover with sealing film, seal the plate, incubate at 37°C for 30 minutes, and wash;

[0111] ⑦ Add 90 μL of substrate solution to each well, shake gently to mix, and add 50 mL of stop solution to each well after color development at 37°C in the dark for 15 minutes to terminate the reaction:

[0112] ⑧Measure the absorbance of each well at 450nm within 10 minutes.

[0113] (4) Record the time the mouse stays on the rotating rod.

[0114] (5) Record the swimming time of mice.

[0115] 1.5 Test results

[0116] (1) RBC and Hb data

[0117] As can be seen from Table 1, compared with the normal group, the RBC and Hb content of the mice in the model group decreased, and the difference was significant, indicating that the model was successfully established. Compared with the model group, the high-dose group of Qi and blood composition, the compound donkey-hide glue slurry group, the composition group 1, and the composition group 2 can all significantly increase the RBC and Hb values ​​of mice (P < 0.01 or P < 0.001), and the effects of the high-dose group of Qi and blood composition and the compound donkey-hide glue slurry group, the composition group 1, and the composition group 2 are not much different. Compared with the high-dose group of Qi and blood composition, there is no significant difference in composition 1-2, and the composition group 3-7 is significant (P < 0.01 or P < 0.001), indicating that each medicinal flavor in the prescription needs to be effective within a certain ratio range. It shows that the high-dose group of Qi and blood composition and the composition groups 1 and 2 can significantly increase the RBC and Hb content of mice with Qi and blood deficiency, and the effect is equivalent to that of positive drugs.

[0118] Table 1 RBC and Hb values ​​of mice in each group after administration ( n=10)

[0119]

[0120]

[0121] Note: Compared with the model group, * p<0.05, ** p<0.01, *** p<0.001;

[0122] Compared with the low-dose group of Qi and Blood Composition, ◆ p<0.05, ◆◆ p<0.01, ◆◆◆ p<0.001;

[0123] Compared with the high-dose group of Qi-blood composition, # p<0.05, ## p<0.01, ### p<0.001.

[0124] (2) EPO, IL-2, and IL-1β data

[0125] As can be seen from Table 2, compared with the normal control group, the IL-2 and TNF-α contents in the model group were significantly increased, and the EPO content was significantly decreased, indicating that the model was successfully established. Compared with the model group, the high-dose group of Qi and blood composition, the compound donkey-hide glue slurry group, the composition group 1, and the composition group 2 can significantly increase the EPO content and significantly reduce the IL-2 and TNF-α contents (P < 0.01) (P < 0.01, P < 0.001), and the high-dose group of Qi and blood composition is equivalent to the compound donkey-hide glue slurry group, the composition group 1, and the composition group 2. Compared with the high-dose group of Qi and blood composition, there is no significant difference in composition 1-2, and the composition group 3-7 is significant (P < 0.05, P < 0.01, P < 0.001), indicating that each medicinal flavor in the prescription needs to be effective within a certain ratio range. It shows that the high-dose group of Qi and blood composition and the composition groups 1 and 2 can significantly increase the EPO content of mice with Qi and blood deficiency, reduce the IL-2 and TNF-α contents, and the effect is equivalent to that of positive drugs.

[0126] Table 2 EPO, IL-2, TNF-α values ​​in each group of mice after administration ( n=10)

[0127]

[0128]

[0129] Note: Compared with the model group, * p<0.05, ** p<0.01, *** p<0.001;

[0130] Compared with the low-dose group of Qi and Blood Composition, ◆ p<0.05, ◆◆ p<0.01, ◆◆◆ p<0.001;

[0131] Compared with the high-dose group of Qi-blood composition, # p<0.05, ## p<0.01, ### p<0.001.

[0132] (3) Rotarod and swimming time data

[0133] As can be seen from Table 3, compared with the normal group, the model group mice's time on the rotarod and swimming time were significantly reduced, indicating that the model was successfully established. Compared with the model group, the compound donkey-hide glue slurry group, the high-dose group of qi and blood composition, the composition group 1, and the composition group 2 all significantly increased the rotarod and swimming time of mice (P < 0.001), and the efficacy was significantly better than the low-dose group of qi and blood composition. The high-dose group of qi and blood composition was equivalent to the compound donkey-hide glue slurry group, the composition group 1, and the composition group 2. Compared with the high-dose group of qi and blood composition, there was no significant difference in composition 1-2, and the composition group 3-7 was significant (P < 0.05, P < 0.01, P < 0.001), indicating that each medicinal flavor in the prescription needs to be effective within a certain ratio range. It shows that the high-dose group of qi and blood composition and the composition groups 1 and 2 significantly increase the time on the rotarod and swimming time of mice with qi and blood deficiency, and the effect is equivalent to that of positive drugs.

[0134] Table 3 Rotarod and swimming time of mice in each group after drug administration ( n=10)

[0135]

[0136] Note: Compared with the model group, * p<0.05, ** p<0.01, *** p<0.001;

[0137] Compared with the low-dose group of Qi and Blood Composition, ◆ p<0.05, ◆◆ p<0.01, ◆◆◆ p<0.001;

[0138] Compared with the high-dose group of Qi-blood composition, # p<0.05, ## p<0.01, ### p<0.001.

[0139] 2. Research on the efficacy of beauty and skin care

[0140] 2.1. Construction of mouse model for delaying skin aging

[0141] D-galactose combined with ultraviolet irradiation was used to induce mouse aging model. Kunming mice were selected, all female, weighing 18-22g, and adaptively fed for 3 days. They were randomly divided into 11 groups according to their body weight, with 10 mice in each group, namely blank control group, model control group, positive control group (Bazi Bushen Capsule), low-dose group of Qi and blood composition, high-dose group of Qi and blood composition, composition group 1, composition group 2, composition group 3, composition group 4, composition group 5, composition group 6 and composition group 7. Except for the low-dose group of Qi and blood paste prescription, the dosage of the other groups was 10g / kg. The back of the mouse was depilated about 5cm×5cm. Except for the blank group, 1% D-galactose 1mL / 100g was subcutaneously injected into the back of the neck every day in other groups, and UVB ultraviolet irradiation was performed for 30min / day for 42 days to cause a subacute aging model. The blank group was injected with the same dose of normal saline. Each group was gavaged with drugs at the same time as modeling. The model group and the blank group were gavaged with an equal amount of normal saline, and the other groups were gavaged with the prepared combination. The positive group was gavaged with 10 g·kg of Ba Zi Bushen Capsule. -1 ·d -1 , once a day for 42 consecutive days. One hour after the last administration of each drug group, the dorsal hair was removed, the dorsal skin was cut and defatted, and the tissue was homogenized to obtain the supernatant to detect the content of SOD and MDA.

[0142] 2.2 Drug preparation and daily dosage

[0143] (1) Blank control group: normal saline, 10 g kg -1 ·d -1 .

[0144] (2) Model control group: normal saline, 10 g kg -1 ·d -1 .

[0145] (3) Bazi Bushen Capsule Group (positive control): Bazi Bushen Capsule, dosage: 10 g kg -1 ·d -1 , dissolved in saline.

[0146] (4) Qi-blood composition low-dose group: the product prepared in Example 1, dosage: 5 g·kg -1 ·d -1 , dissolved in saline.

[0147] (5) Qi and blood composition high dose group: the product prepared in Example 1, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0148] (6) Composition 1: the product prepared in Example 2, dosage: 10 g·kg -1 ·d -1, dissolved in saline.

[0149] (7) Composition Group 2: the product prepared in Example 3, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0150] (8) Composition group 3: the product prepared in Example 4, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0151] (9) Composition Group 4: product prepared in Example 5, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0152] (10) Composition Group 5: the product prepared in Comparative Example 1, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0153] (11) Composition group 6: product prepared in comparative example 2, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0154] (12) Composition group 7: product prepared in comparative example 3, dosage: 10 g·kg -1 ·d -1 , dissolved in saline.

[0155] 2.3. Dosage

[0156] All animals were given an equal amount of ordinary feed + drinking water. According to the above daily dosage, each group of drugs was made into a solution and administered by gavage at 20 mL / kg for 10 consecutive days.

[0157] 2.4、Detection indicators

[0158] (1) ELISA method to detect the content of SOD and MDA in tissues:

[0159] One hour after the last administration, the mouse skin tissue was taken, added with an appropriate amount of PBS solution and mashed, and centrifuged at 3000 rpm for 10 minutes to obtain the supernatant, and the SOD and MDA levels in the serum were detected.

[0160] ① Standard curve determination: Take the mother solution of each standard according to the instructions of the kit, add the standard diluent to dilute it to 5 concentrations in turn, take 100μL of the diluted standard and add it to the coated ELISA plate;

[0161] ② Add 100 μL of the sample to be tested to the test well, cover with a sealing film, seal the plate and incubate at 37°C for 90 min;

[0162] ③ Add 100 μL of biotinylated antibody to each well, cover with sealing film, seal the plate and incubate at 37°C for 60 min;

[0163] ④ Liquid preparation: dilute the concentrated detergent with distilled water to make 1x application solution for later use;

[0164] ⑤ Washing: Remove the sealing film, discard the liquid in the wells of the ELISA plate, spin dry, fill each well with washing solution, let it stand for 30 seconds and then discard it, repeat 5 times, and pat the ELISA plate dry;

[0165] ⑥Add 100 μL of enzyme binding working solution to each well, cover with sealing film, seal the plate and incubate at 37°C for 30 minutes.

[0166] washing;

[0167] ⑦ Add 90 μL of substrate solution to each well, shake gently to mix, and add 50 mL of stop solution to each well after color development at 37°C in the dark for 15 minutes to terminate the reaction:

[0168] ⑧Measure the absorbance of each well at 450nm within 10 minutes.

[0169] 2.5 Test results

[0170] (1) SOD and MDA data

[0171] As can be seen from Table 4, compared with the normal group, the SOD content of mice in the model group was significantly reduced (P < 0.001), while the MDA content was significantly increased (P < 0.001), indicating that the model was successfully established. Compared with the model group, the SOD content of mice in the compound donkey-hide glue group, the high-dose group of qi and blood composition, the composition group 1, and the composition group 2 was extremely significantly increased (P < 0.001), and the MDA content was extremely significantly reduced (P < 0.001). Compared with the model group, there was no significant change in the SOD and MDA content of mice in the low-dose group of qi and blood composition. Compared with the high-dose group of qi and blood composition, there was no significant difference in composition 1-2, and the composition group 3-7 was significant (P < 0.05, P < 0.01, P < 0.001), indicating that each medicinal flavor in the prescription needs to be effective within a certain ratio range. It shows that the high-dose group of qi and blood composition and the composition groups 1 and 2 significantly increase the MDA content of aging mice, significantly reduce the SOD content, and delay the aging of mouse skin, and the effect is equivalent to that of positive drugs.

[0172] Table 4 SOD and MDA values ​​of mice in each group after administration ( n=10)

[0173]

[0174] Note: Compared with the model group, * p<0.05, **p<0.01, *** p<0.001;

[0175] Compared with the low-dose group of Qi and Blood Composition, ◆ p<0.05, ◆◆ p<0.01, ◆◆◆ p<0.001;

[0176] Compared with the high-dose group of Qi-blood composition, # p<0.05, ## p<0.01, ### p<0.001.

[0177] In summary, the Chinese medicine composition disclosed in the present invention can significantly inhibit the reduction of RBC and Hb caused by deficiency of both qi and blood, increase EPO in mouse serum, reduce IL-2 and TNF-α content, significantly prolong the time of mouse rotarod and weight-bearing swimming, and has multiple effects such as replenishing qi and blood, strengthening spleen and lung, calming the mind and eliminating vexation, nourishing yin and moistening dryness, and is suitable for a variety of diseases such as qi and blood deficiency and oxidative aging caused by insufficient qi and blood. In terms of therapeutic effect, the efficacy of the composition with the best ratio is equivalent to that of the positive drug (compound donkey-hide gelatin syrup / eight seeds kidney-tonifying capsule).

[0178] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for invigorating qi and nourishing blood, beautifying and nourishing the skin, characterized in that: The composition comprises: donkey-hide gelatin, longan pulp, hawthorn, yam, astragalus, rose, mulberry, codonopsis, tuckahoe, raspberry, liquorice, jujube, red ginseng and ginger.

2. The composition according to claim 1, characterized in that The composition comprises, by weight, 0.5-6 parts of donkey-hide gelatin, 0.5-9 parts of longan pulp, 2-12 parts of hawthorn, 22-55 parts of yam, 45-72 parts of astragalus, 2-12 parts of rose, 1-10 parts of mulberry, 14-32 parts of codonopsis, 27-46 parts of poria, 0.5-6 parts of raspberry, 14-28 parts of liquorice, 2-12 parts of jujube, 0.5-6 parts of red ginseng and 28-42 parts of ginger.

3. The composition according to claim 2, characterized in that The composition comprises, by weight, 1-5 parts of donkey-hide gelatin, 1-8 parts of longan pulp, 4-10 parts of hawthorn, 30-50 parts of yam, 50-70 parts of astragalus, 4-10 parts of rose, 2-8 parts of mulberry, 15-30 parts of codonopsis, 30-44 parts of poria, 1-5 parts of raspberry, 15-25 parts of liquorice, 4-10 parts of jujube, 1-5 parts of red ginseng and 30-40 parts of ginger.

4. A method for preparing the composition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: adding water to a prescribed amount of medicinal materials to extract the extract.

5. The preparation method according to claim 4, characterized in that: The specific steps include: Add water, heat and reflux to extract 2-4 times, add 5-15 times the weight of the medicinal material water each time, extract for 1-3 hours each time to obtain an extract.

6. The preparation method according to claim 5, characterized in that: The specific steps include: Add water and heat under reflux for extraction twice. For the first time, add 12 times the weight of water of the medicinal material and extract for 80 minutes. For the second time, add 10 times the weight of water of the medicinal material and extract for 40 minutes to obtain an extract.

7. The preparation method according to claim 4, characterized in that: The preparation method further comprises the following steps: concentrating the extract under reduced pressure into a thick paste, drying under reduced pressure, and crushing into powder to obtain the extract.

8. The preparation method according to claim 7, characterized in that: The preparation method further comprises the following steps: concentrating the extract under reduced pressure to a thick paste with a relative density of 1.2-1.3 (thermal measurement at 50-60° C.), drying under reduced pressure at 60-70° C., and crushing into powder to obtain the extract.

9. A medicine, the active ingredient of which comprises the composition according to any one of claims 1 to 3, or the extract prepared by the preparation method according to any one of claims 4 to 8.

10. Use of the composition according to any one of claims 1 to 3, or the extract prepared by the preparation method according to any one of claims 4 to 8 in the preparation of products for invigorating qi and nourishing blood, and beautifying and nourishing the skin.

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