Composition for promoting regeneration of dental pulp and dentin

Abstract

The invention relates to the technical field of dental treatment, in particular to a composition for promoting dental pulp and dentin regeneration, which comprises a growth factor, a mineral regeneration enhancer and an embedded stent, the growth factor is a composition of a vascular endothelial growth factor, a platelet-derived growth factor, an epidermal growth factor, a granulocyte colony stimulating factor and a bone morphogenetic protein; the content ratios of the vascular endothelial growth factor, the platelet-derived growth factor, the epidermal growth factor, the granulocyte colony stimulating factor and the bone morphogenetic protein are 12%-24%, 20%-42%, 6%-12%, 2%-7% and 8%-14% respectively, and the embedded stent comprises the following components: chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots. The content ratios of the chitosan, the bacterial cellulose, the hyaluronic acid, the hydrogel, the chondroitin sulfate and the natural blood clots are respectively 18%-33%, 10%-22%, 3%-9%, 2%-6%, 2%-8% and 7%-22%; the dental pulp regeneration effect after root canal treatment can be remarkably improved, dentin mineralization is promoted, and wound healing is accelerated.

Description

Technical Field

[0001] The invention relates to a composition for promoting the regeneration of dental pulp and dentin, belonging to the technical field of dental treatment. Background Art

[0002] The dental pulp is the soft tissue inside the tooth, mainly composed of nerves, blood vessels, lymph and connective tissue, etc. It is located in the dental pulp cavity and has the functions of nutrition, sensation, defense and formation of dentin. Dentin is the main part of the tooth, hard and elastic, located in the inner layer of enamel and cementum, and runs through the entire crown and root. It supports the enamel and also provides protection for the dental pulp. The regeneration of the dental pulp and dentin is to restore the vitality and structural integrity of the damaged teeth, prevent further infection and damage, ensure the normal function of the teeth, and improve the quality of life of patients. As disclosed in announcement number (CN104548212A), a composition for promoting pulp and dentin regeneration is disclosed, including platelet-derived growth factor PDGF and a scaffold material, wherein the platelet-derived growth factor PDGF is one or more of PDGF-BB, PDGF-AB and PDGF-AA, and the scaffold material is one or more of alginate, gum arabic, guar gum, xanthan gum, gelatin, chitin, chitosan, chitosan acetate, chitosan lactate, chondroitin sulfate, N, O-carboxymethyl chitosan, dextran, fibrin gel, fibrinogen, natural blood clot, glycerol, hyaluronic acid, quinone, glucosamine, proteoglycan, starch, lactic acid, poloxamer, sodium glycerophosphate, collagen, glycogen, keratin filaments, synthetic surgical sealants and adhesives. The invention can be conveniently injected into the dental pulp cavity that needs treatment, and through the combined action of platelet-derived growth factor (PDGF) and the scaffold material, the repair cells enter the pulp cavity and play a repairing role, thereby regenerating and repairing the diseased dental pulp and dentin.

[0003] However, when the above technical solution is used, it adapts a single platelet-derived growth factor to promote tooth regeneration. Although it can accelerate cell proliferation and differentiation, it cannot ensure the supply of nutrients. The wound healing of the new tissue is slow, it is more difficult to regulate the immune response, and the mineralization process of the dentin is slow, which affects the efficiency of regeneration.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a composition for promoting pulp and dentin regeneration in order to solve the above problems, which has the advantages of significantly improving the pulp regeneration effect after root canal treatment, promoting dentin mineralization, accelerating wound healing, and effectively restoring the health of teeth.

[0006] The present invention achieves the above-mentioned purpose through the following technical scheme: a composition for promoting pulp and dentin regeneration, comprising growth factors, mineral regeneration enhancers and embedded scaffolds, wherein the growth factors are a composition of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, wherein the content of the vascular endothelial growth factor, the platelet-derived growth factor, the epidermal growth factor, the granulocyte colony stimulating factor and the bone morphogenetic protein is 12%-20%, respectively. 24%, 20%-42%, 6%-12%, 2%-7% and 8%-14%, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots, the chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clot content accounted for 18%-33%, 10%-22%, 3%-9%, 2%-6%, 2%-8% and 7%-22%, respectively, and the embedded scaffold surface is modified.

[0007] Furthermore, in order to promote the regeneration and strengthening of tooth minerals and effectively repair tooth enamel damage, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, and the hydroxyapatite and the tricalcium phosphate are mixed in equal amounts to simulate natural dentin components.

[0008] Furthermore, in order to enhance the affinity and interaction between the embedded scaffold and the cells, thereby promoting the adhesion, proliferation and differentiation of cells on the scaffold and accelerating the regeneration process of dental pulp and dentin, the surface of the embedded scaffold is plasma treated to change the chemical properties and microstructure of the surface of the embedded scaffold, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded scaffold and the cells.

[0009] Furthermore, in order to promote angiogenesis and ensure nutrient supply, stimulate cell activity to accelerate proliferation and differentiation, and improve wound healing efficiency, and jointly promote rapid and healthy regeneration and repair of tissues, the vascular endothelial growth factor provides sufficient nutrient supply for regenerated tissues; the platelet-derived growth factor is used to stimulate cell proliferation and differentiation; and the epidermal growth factor is used to heal wounds.

[0010] Furthermore, in order to precisely regulate the immune response and maintain the balance in the body, and at the same time to construct a strong and stable tissue structure, the granulocyte colony stimulating factor is used to regulate the immune response; the bone morphogenetic protein is used to mineralize the bone and dentin to construct a strong tissue structure.

[0011] Furthermore, in order to create an ideal tissue regeneration microenvironment, the chitosan provides a three-dimensional porous scaffold environment on which dental pulp stem cells can attach, proliferate and differentiate into the desired tissue type; the bacterial cellulose is used to provide support for cell growth; the hyaluronic acid is used to simulate the extracellular matrix environment; and the hydrogel is used to maintain a moist environment and control the release of growth factors.

[0012] Furthermore, in order to enhance the biocompatibility and functionality of the regenerated dentin, the chondroitin sulfate is used to simulate natural dentin matrix components; the natural blood clot contains growth factors and extracellular matrix components.

[0013] Furthermore, in order to accurately promote pulp regeneration and dentin repair and restore the health of teeth, the growth factor, the mineral regeneration enhancer and the embedded scaffold are chemically cross-linked to form a complex with release properties, which is used for local filling of the pulp cavity or exposed dentin surface after root canal treatment.

[0014] The technical effects and advantages of the present invention are as follows: through the synergistic effect of growth factors such as vascular endothelial growth factor, comprehensive nutritional support and growth regulation are provided for pulp and dentin regeneration. At the same time, the three-dimensional porous scaffold constructed by biomaterials such as chitosan and bacterial cellulose not only provides a superior environment for cell growth, but also further enhances the biocompatibility and functionality of the scaffold through modification treatment, which can significantly improve the pulp regeneration effect after root canal treatment, promote dentin mineralization, accelerate wound healing, and effectively restore the health of teeth. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is a comparative table of embodiments of the present invention;

[0016] Figure 2 Schematic diagram of the components of the growth factor of the present invention;

[0017] Figure 3 Schematic diagram of the components of the embedded scaffold of the present invention. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Embodiment 1

[0020] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 12%, 20%, 6%, 2% and 8% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfur The contents of acid chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots are 18%, 10%, 3%, 2%, 2% and 7% respectively, and the surface of the embedded bracket is modified, and the surface of the embedded bracket is plasma treated to change the chemical properties and microstructure of the surface of the embedded bracket, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded bracket and cells. Growth factors, mineral regeneration enhancers and embedded brackets form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 738 hours, which is highly efficient.

[0021] Vascular endothelial growth factor provides sufficient nutrition for regenerating tissue; platelet-derived growth factor is used to stimulate cell proliferation and differentiation; epidermal growth factor is used to heal wounds, and granulocyte colony-stimulating factor is used to regulate immune response; bone morphogenetic protein is used to mineralize bone and dentin and build a strong tissue structure.

[0022] Chitosan provides a three-dimensional porous scaffold environment on which dental pulp stem cells can attach, proliferate and differentiate into the desired tissue type; bacterial cellulose is used to provide support for cell growth; hyaluronic acid is used to simulate the extracellular matrix environment; hydrogel is used to maintain a moist environment and control the release of growth factors, and chondroitin sulfate is used to simulate natural dentin matrix components; natural blood clots contain growth factors and extracellular matrix components.

[0023] Embodiment 2

[0024] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 14%, 22%, 7%, 3% and 10% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfuric acid The contents of chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots account for 21%, 12%, 4%, 3%, 3% and 11% respectively, and the surface of the embedded scaffold is modified, and the surface of the embedded scaffold is plasma treated to change the chemical properties and microstructure of the surface of the embedded scaffold, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded scaffold and cells. Growth factors, mineral regeneration enhancers and embedded scaffolds form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 756 hours, which is highly efficient.

[0025] Embodiment 3

[0026] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 16%, 23%, 7%, 3% and 11% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfuric acid The contents of chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots account for 22%, 14%, 6%, 3%, 5% and 17% respectively, and the surface of the embedded scaffold is modified, and the surface of the embedded scaffold is plasma treated to change the chemical properties and microstructure of the surface of the embedded scaffold, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded scaffold and cells. Growth factors, mineral regeneration enhancers and embedded scaffolds form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 682 hours, which is highly efficient.

[0027] Embodiment 4

[0028] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 18%, 24%, 9%, 5% and 11% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfur The contents of acid chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots are 25%, 16%, 8%, 4%, 6% and 19% respectively, and the surface of the embedded bracket is modified, and the surface of the embedded bracket is plasma treated to change the chemical properties and microstructure of the surface of the embedded bracket, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded bracket and cells. Growth factors, mineral regeneration enhancers and embedded brackets form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 668 hours, which is highly efficient.

[0029] Embodiment 5

[0030] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 21%, 32%, 9%, 5% and 13% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfur The contents of acid chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots are 28%, 17%, 7%, 5%, 7% and 20% respectively, and the surface of the embedded bracket is modified, and the surface of the embedded bracket is plasma treated to change the chemical properties and microstructure of the surface of the embedded bracket, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded bracket and cells. Growth factors, mineral regeneration enhancers and embedded brackets form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 702 hours, which is highly efficient.

[0031] Embodiment 6

[0032] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 22%, 38%, 11%, 6% and 13% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfur The contents of acid chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots are 31%, 21%, 6%, 5%, 7% and 21% respectively, and the surface of the embedded bracket is modified, and the surface of the embedded bracket is plasma treated to change the chemical properties and microstructure of the surface of the embedded bracket, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded bracket and cells. Growth factors, mineral regeneration enhancers and embedded brackets form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 712 hours, which is highly efficient.

[0033] Embodiment 7

[0034] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 24%, 42%, 12%, 7% and 14% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfur The contents of acid chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots are 33%, 22%, 9%, 6%, 8% and 22% respectively, and the surface of the embedded bracket is modified, and the surface of the embedded bracket is plasma treated to change the chemical properties and microstructure of the surface of the embedded bracket, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded bracket and cells. Growth factors, mineral regeneration enhancers and embedded brackets form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 692 hours, which is highly efficient.

[0035] Comparative Example 1

[0036] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 8%, 18%, 5%, 1% and 5% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfuric acid The contents of chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots are 15%, 7%, 2%, 1%, 1% and 5% respectively, and the surface of the embedded scaffold is modified, and the surface of the embedded scaffold is plasma treated to change the chemical properties and microstructure of the surface of the embedded scaffold, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded scaffold and the cells. Growth factors, mineral regeneration enhancers and embedded scaffolds form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 1152 hours, and the efficiency is not good.

[0037] Comparative Example 2

[0038] See also Figure 1-Figure 3As shown, a composition for promoting pulp and dentin regeneration includes growth factors, mineral regeneration enhancers and embedded scaffolds, the growth factors are a combination of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein, the contents of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony stimulating factor and bone morphogenetic protein are 28%, 16%, 13%, 8% and 17% respectively, the mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, hydroxyapatite and tricalcium phosphate are in equal proportions, and are used to simulate natural dentin components, the embedded scaffold components include chitosan, bacterial cellulose, hyaluronic acid, hydrogel, sulfur Acid chondroitin and natural blood clots, chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clots account for 16%, 9%, 10%, 9%, 9% and 24% respectively, and the surface of the embedded bracket is modified, and the surface of the embedded bracket is plasma treated to change the chemical properties and microstructure of the surface of the embedded bracket, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded bracket and the cells. Growth factors, mineral regeneration enhancers and embedded brackets form a complex with release characteristics through chemical cross-linking, which is used for the pulp cavity or exposed dentin surface after root canal treatment. It is achieved by local filling. The pulp and dentin regeneration time is 1180 hours, and the efficiency is not good.

[0039] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0040] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A composition for promoting pulp and dentin regeneration, comprising growth factors, mineral regeneration enhancers and embedded scaffolds, characterized in that: The growth factor is a composition of vascular endothelial growth factor, platelet-derived growth factor, epidermal growth factor, granulocyte colony-stimulating factor and bone morphogenetic protein, and the contents of the vascular endothelial growth factor, the platelet-derived growth factor, the epidermal growth factor, the granulocyte colony-stimulating factor and the bone morphogenetic protein are 12%-24%, 20%-42%, 6%-12%, 2%-7% and 8%-14%, respectively. The embedded scaffold component includes chitosan, bacterial cellulose, hyaluronic acid, hydrogel, chondroitin sulfate and natural blood clot, and the contents of the chitosan, the bacterial cellulose, the hyaluronic acid, the hydrogel, the chondroitin sulfate and the natural blood clot are 18%-33%, 10%-22%, 3%-9%, 2%-6%, 2%-8% and 7%-22%, respectively, and the surface of the embedded scaffold is modified.

2. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The mineral regeneration enhancer is composed of hydroxyapatite and tricalcium phosphate, which are mixed in equal proportions and are used to simulate natural dentin components.

3. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The surface of the embedded stent is subjected to plasma treatment to change the chemical properties and microstructure of the surface of the embedded stent, and active hydroxyl groups are introduced to change the interaction between the surface of the embedded stent and cells.

4. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The vascular endothelial growth factor provides sufficient nutrition for regenerating tissue; the platelet-derived growth factor is used to stimulate cell proliferation and differentiation; and the epidermal growth factor is used to heal wounds.

5. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The granulocyte colony stimulating factor is used to regulate immune response; the bone morphogenetic protein is used to mineralize bone and dentin to build a strong tissue structure.

6. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The chitosan provides a three-dimensional porous scaffold environment, allowing dental pulp stem cells to attach and proliferate thereon and differentiate into the desired tissue type; the bacterial cellulose is used to provide support for cell growth; the hyaluronic acid is used to simulate the extracellular matrix environment; and the hydrogel is used to maintain a moist environment and control the release of growth factors.

7. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The chondroitin sulfate is used to simulate natural dentin matrix components; the natural blood clot contains growth factors and extracellular matrix components.

8. The composition for promoting dental pulp and dentin regeneration according to claim 1, characterized in that: The growth factor, the mineral regeneration enhancer and the embedded scaffold form a composite with release characteristics through chemical cross-linking, which is used in the dental pulp cavity or exposed dentin surface after root canal treatment and is achieved through local filling.

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