Preservation and observation device for tiny insect liquid immersion specimens

Abstract

A preservation and observation device for tiny insect liquid immersion specimens relates to the technical field of insect research and comprises a specimen preservation bin and a sealing cover, the specimen preservation bin comprises a columnar preservation and observation bin and a columnar buffering bin, a bin opening of the preservation and observation bin is connected with the bin bottom of the buffering bin, and communication between the preservation and observation bin and the buffering bin is achieved. The cross section area of the storage and observation bin is smaller than that of the buffer bin, the storage and observation bin and the buffer bin are filled with storage liquid, and a sealing assembly used for blocking a bin opening of the storage and observation bin is arranged in the buffer bin. The technical problems that an existing preservation device is difficult to directly observe a specimen under a high-power microscope, and the observation precision is not high can be solved.

Description

Technical Field

[0001] The invention relates to the technical field of insect research, in particular to a device for preserving and observing tiny insect liquid-immersion specimens. Background Art

[0002] Liquid immersion is one of the important methods for preserving insect specimens. It is widely used to preserve insect specimens of various sizes, shapes, and stages, especially tiny insects and their larvae, pupae, and other stages or tissues with high moisture content. Compared with dry specimen preservation methods such as pin insertion, liquid immersion can better preserve the soft tissue and color of insects, and its advantages are more obvious for preserving transparent or translucent insect bodies and tissues.

[0003] When using liquid immersion specimen preservation technology to preserve tiny insects, the traditional method is usually to use a glass tube or glass bottle, first inject ethanol, formalin or other preservation fixatives, and then place the insect specimens in it. However, this traditional liquid immersion specimen preservation device has obvious shortcomings, which affects the preservation and observation of specimens.

[0004] First, traditional preservation devices mostly use glass tubes or small glass bottles as containers. Although these materials have good corrosion resistance and transparency, their sealing performance is often insufficient. Preservative solutions such as ethanol or formalin are prone to gradually evaporate during long-term storage due to loose sealing of the container, resulting in changes in the composition of the preservation solution or a drop in the liquid level, which in turn causes the specimen to rot or dry out and deform.

[0005] Secondly, traditional liquid immersion preservation devices, such as glass bottles and glass tubes, have many inconveniences when observing liquid immersion specimens. Specimens stored in wide-mouthed glass bottles need to be taken out and placed in culture dishes or wax trays when observed. This process may not only damage the specimens, but also cause the preservation solution to evaporate and release pungent odors. Although some glass tubes, ampoules, etc. can be directly placed under a magnifying glass or microscope for observation, the specimens are often floating in the preservation solution and are not easy to fix. The observation field needs to be adjusted continuously, which is very inconvenient. When encapsulating liquid immersion specimens, it is found that when the preservation solution is poured into containers such as glass tubes, the rubber ring in the tube cap will inevitably leave air at the moment of covering the tube mouth, which makes it difficult for the liquid to completely fill the entire device, and it is almost impossible to avoid the generation of bubbles. In addition, since the specimens may be stored for decades, the rubber ring may age and the wax seal may fall off during this process, which will cause the preservation solution to evaporate, causing air to enter the container and form bubbles. When observing insects in a test tube under a microscope, the test tube needs to be placed horizontally to observe the insects inside the tube. These bubbles not only block the line of sight, but also interfere with the position and clarity of the specimen. These problems are more serious for tiny insects, making it extremely difficult to directly observe the specimens under a high-power microscope using traditional preservation devices, seriously affecting the accurate observation of their fine structures.

[0006] To sum up, the above-mentioned shortcomings of traditional preservation devices limit their application effects in high-precision observation and long-term preservation. At present, there is no ideal preservation and observation device for tiny insect liquid-immersed specimens. Most of the existing related technologies are specimen boxes for placing preservation devices, rather than preservation and observation devices for direct liquid-immersed specimens, such as patent No. CN202023264399.4, the patent name is a Chinese utility model patent for a preservation device for small insect immersed specimens, and patent No. CN202010508045.5, the patent name is a Chinese utility model patent for an insect liquid-immersed specimen box.

[0007] Therefore, there is an urgent need for an improved liquid immersion specimen preservation and observation device that can provide a more realistic and clear observation effect while maintaining the integrity of the specimen. Summary of the invention

[0008] The present invention aims to provide a storage and observation device for liquid-immersion specimens of tiny insects, so as to solve the technical problems that the existing storage device is difficult to directly observe the specimens under a high-power microscope and the observation accuracy is not high.

[0009] In order to achieve the above-mentioned purpose, the specific scheme adopted by the present invention is: a preservation and observation device for liquid-immersed specimens of tiny insects, including a specimen preservation chamber and a sealing cover, the specimen preservation chamber including a columnar preservation and observation chamber and a columnar buffer chamber, the chamber opening of the preservation and observation chamber is connected to the chamber bottom of the buffer chamber, and the preservation and observation chamber and the buffer chamber are connected, the cross-sectional area of ​​the preservation and observation chamber is smaller than the cross-sectional area of ​​the buffer chamber, the preservation and observation chamber and the buffer chamber are both filled with preservation liquid, and a sealing component for sealing the chamber opening of the preservation and observation chamber is arranged in the buffer chamber.

[0010] As a further optimization of the above technical solution, the sealing assembly includes a fixing rod and a sealing plug arranged at one end of the fixing rod, and an end of the fixing rod away from the sealing plug can abut against the sealing cover.

[0011] As a further optimization of the above technical solution, the cross-sections of the buffer bin and the sealing plug are both circular, the diameter of the sealing plug is smaller than the inner diameter of the buffer bin, and the overall height of the sealing plug and the fixing rod is greater than the height of the buffer bin.

[0012] As a further optimization of the above technical solution, the bottom of the buffer bin is provided with a plurality of circumferentially spaced positioning protrusions, and the inner edges of the plurality of positioning protrusions are in contact with the side surface of the sealing plug.

[0013] As a further optimization of the above technical solution, the side of the sealing cover is sequentially provided with a threaded section threadedly connected to the buffer bin and a wax sealing section sleeved on the outside of the buffer bin. The wax sealing section and the outer wall of the buffer bin form a wax sealing groove, and a rubber gasket is provided on the inner side of the top of the sealing cover.

[0014] As a further optimization of the above technical solution, the fixing rod is fixedly connected to the center position of the inner side of the cover top of the sealing cover.

[0015] As a further optimization of the above technical solution, the walls of the storage observation chamber are all flat.

[0016] As a further optimization of the above technical solution, the cross-section of the observation chamber is preserved to be triangular, rectangular or square.

[0017] As a further optimization of the above technical solution, the outer cover of the storage observation chamber is provided with a light-shielding protective cover.

[0018] As a further optimization of the above technical solution, the buffer bin and the light-shielding protective cover are both cylindrical and have the same outer diameter, and the light-shielding protective cover is threadedly connected to the lower part of the buffer bin.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The preservation and observation device provided by the present invention can directly observe while preserving the specimen, effectively reducing problems such as specimen displacement and bubble interference, extending the preservation time, improving the observation effect, and meeting the needs of research and teaching on tiny insects.

[0021] The present invention isolates the connected preservation observation chamber and buffer chamber through a sealing component, which effectively prevents bubbles from entering the preservation observation chamber and affecting the position and shape of the specimen on the one hand, and prevents the specimen from floating and moving in the preservation solution on the other hand, thereby reducing the difficulty of observation under a high-power microscope; and after the buffer chamber is separated from the preservation observation chamber, it is not necessary to completely fill the buffer chamber with the preservation solution, but only needs to be kept above the safety scale, allowing air to exist in the buffer chamber. These air columns or bubbles cannot enter the preservation observation chamber due to the obstruction of the sealing component, and thus cannot affect the observation; at the same time, it is also possible to judge whether the device is leaking based on the change of the liquid level in the buffer chamber, and even if there is a leak, it will not directly affect the preservation observation chamber, thereby playing a role of buffering and prompting.

[0022] The storage and observation chamber of the present invention is mainly used for observing tiny insects. The size of the observation chamber is extremely narrow. Such a narrow transparent glass structure is very easy to break and wear during the storage and taking process. Therefore, it is supported on a cylindrical structure (i.e., a buffer chamber) with the same size as a conventional test tube, and a light-shielding protective cover of the same size is also provided on the outside. This design not only solves the problem of bubbles affecting observation, but also can be adapted to conventional test tube racks and specimen boxes, which is conducive to the conventional storage and management of specimen tubes.

[0023] Since light can cause insect specimens to change color, fade, and even change their tissue and molecular structure, the design of the light-shielding cover effectively reduces the impact of light on the specimens and helps maintain the stability of the specimens under different environmental conditions. After installing the light-shielding cover, it not only provides additional physical protection for the storage observation chamber, but also makes the size of the entire device consistent with the common cryopreservation tubes on the market, and can be directly adapted to existing tube racks and specimen boxes, thereby reducing the cost of device replacement and matching.

[0024] The present invention effectively reduces the influence of optical distortion when observing under a microscope and improves the observation accuracy of specimens under a microscope by adopting a storage observation chamber with a flat wall design. This is particularly important for the study of the fine structure of tiny insects, ensuring higher quality microscopic images and more accurate research results. Compared with cylindrical test tubes, observation chambers with a flat wall design are more susceptible to damage during the storage process. The combination of the light-shielding protective cover and the storage observation chamber not only solves the influence of optical distortion on specimen observation and the influence of light irradiation on specimen preservation, but also compensates for the irregularities in the structure of the device and improves the protection of the specimen, making the device more adaptable to the needs of standardized storage and specimen preservation. At the same time, the modular combination of the two also realizes the flexible switching of specimen preservation function and observation function, significantly enhancing the practicality and functionality of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the storage observation device of the present invention;

[0026] Figure 2 This is a schematic diagram of the structure of the specimen storage chamber;

[0027] Figure 3 is a schematic diagram of the structure of the sealing component;

[0028] Figure 4 is a top view schematic diagram of a sealing assembly;

[0029] Figure 5 This is a top view of the specimen storage chamber;

[0030] Figure 6 is a cross-sectional schematic diagram of a sealing cover;

[0031] Figure 7 is a bottom view schematic diagram of the sealing cover;

[0032] Figure numerals: 1. Sealing cover, 101. Cover top, 102. Threaded section, 103. Wax sealing section, 104. Rubber gasket, 105. Fixing ring, 106. Wax sealing groove, 2. Specimen storage chamber, 201. Storage observation chamber, 202. Buffer chamber, 3. Sealing assembly, 301. Sealing plug, 302. Fixing rod, 4. Light-shielding protective cover, 5. Specimen, 6. Positioning protrusion, 7. First external thread, 8. Second external thread. DETAILED DESCRIPTION

[0033] The technical solution of the present invention is further elaborated in detail below in conjunction with specific embodiments. The parts of the present invention that are not described and disclosed in detail in the following embodiments should be understood as the prior art known or should be known to those skilled in the art.

[0034] like Figure 1 , 2 As shown, the present invention discloses a storage and observation device for liquid-immersed specimens of tiny insects, including a specimen storage chamber 2 and a sealing cover 1. The specimen storage chamber 2 is made of a transparent hard material and consists of an upper buffer chamber 202 and a lower storage and observation chamber 201. The storage and observation chamber 201 is used to hold tiny insects to be kept and observed (i.e., specimens 5).

[0035] Specifically, the preservation observation bin 201 and the buffer bin 202 are both columnar and coaxially distributed, the bin opening of the preservation observation bin 201 is connected to the bin bottom of the buffer bin 202, and the preservation observation bin 201 and the buffer bin 202 are connected, the cross-sectional area of ​​the preservation observation bin 201 is smaller than the cross-sectional area of ​​the buffer bin 202, the preservation observation bin 201 and the buffer bin 202 are both filled with preservation liquid, and the buffer bin 202 is provided with a sealing component 3 for sealing the bin opening of the preservation observation bin 201.

[0036] like Figure 2 , 3 As shown in Figure 4, the sealing assembly 3 includes a fixed rod 302 and a sealing plug 301 arranged at one end of the fixed rod 302, and the end of the fixed rod 302 away from the sealing plug 301 abuts against the sealing cover 1. The sealing plug 301 is arranged at the lower end of the fixed rod 302, and a gap is left between the sealing plug 301 and the inner wall of the buffer bin 202, so that the sealing plug 301 can move up and down in the buffer bin 202. In this embodiment, the sealing plug 301 is a cylindrical rubber plug, the buffer bin 202 is cylindrical, and the diameter of the rubber plug is not less than 2 / 3 of the diameter of the buffer bin 202. The height of the entire sealing assembly 3 (fixing rod 302 + rubber plug) is slightly higher than the buffer bin 202, so that when the sealing cover 1 is tightened on the buffer bin 202, the sealing plug 301 is squeezed and blocked on the port of the storage observation bin 201, at this time, the buffer bin 202 can be separated from the storage observation bin 201, and the specimen position can be fixed while preventing the bubbles in the buffer bin 202 from entering the observation bin when the device is placed flat.

[0037] like Figure 5 As shown, when the sealing cover 1 is screwed onto the buffer bin 202, in order to prevent the lower end of the fixing rod 302 from deviating from the opening of the preservation observation bin 201 and affecting the sealing effect of the sealing assembly 3 on the preservation observation bin 201, a plurality of circumferentially spaced positioning protrusions 6 are provided at the bottom of the buffer bin 202, and the inner edges of the plurality of positioning protrusions 6 are in contact with the side of the sealing plug 301. In this embodiment, there are four positioning protrusions 6, and the positioning protrusions 6 position and constrain the lower end of the fixing rod 302, and fix the lower end of the fixing rod 302 between the plurality of positioning protrusions 6, thereby maintaining the stability of the lower end of the fixing rod 302, avoiding the sealing plug 301 and the opening of the preservation observation bin 201 from being misaligned during the subsequent long-term preservation of the specimen 5, and improving the sealing effect of the preservation observation bin 201.

[0038] Combination Figure 6 , 7 As shown, the sealing cover 1 is threadedly connected to the port of the buffer bin 202, and a fixing ring 105 for restraining the fixing rod 302 is provided on the inner side of the cover top 101 of the sealing cover 1. The fixing ring 105 is concentrically distributed with the cover top 101, and the fixing rod 302 is a cylindrical hard solid structure. The outer diameter of the fixing rod 302 is slightly smaller than the inner diameter of the fixing ring 105, so that the upper end of the fixing rod 302 can be inserted into the fixing ring 105.

[0039] The side of the sealing cover 1 is provided with a threaded section 102 threadedly connected to the buffer bin 202 and a wax seal section 103 sleeved on the outside of the buffer bin 202 in sequence from top to bottom. The threaded section 102 of the sealing cover 1 is provided with an internal thread, and the outer side wall of the port of the buffer bin 202 is provided with an external thread (i.e., Figure 2 The second external thread 8 shown in the figure, the diameter of the wax seal section 103 is larger than the diameter of the thread section 102 and larger than the outer diameter of the buffer chamber 202, and the wax seal section 103 and the outer wall of the buffer chamber 202 form a wax seal groove 106. When the sealing cover 1 is tightened, the melted wax liquid can be dripped inverted, and the wax forms a sealing layer after solidification, which can effectively prevent the volatilization of the preservation liquid and significantly prolong the preservation time of the specimen 5. A rubber gasket 104 is arranged on the inner side of the cover top 101 of the sealing cover 1, which further enhances the sealing between the sealing cover 1 and the buffer chamber 202.

[0040] The invention introduces the wax seal groove 106 design to further enhance the sealing performance of the device, forming a double sealing mechanism, effectively preventing the volatilization of the preservation solution and the invasion of external air and impurities. When the traditional method is used for wax sealing, wax is usually dripped directly on the tube mouth to form a plug-shaped sealing structure or the sealed tube is immersed in melted wax liquid. The first method requires the specimen tube to be sealed with cotton or the like first, and then the wax liquid is dripped into the sealing wax layer, which is suitable for the sealing of dried specimens; although the second method can form a sealing wax film near the tube mouth, it is directly exposed to the outside and is very easy to wear and fall off, and the thickness is difficult to control, and the preservation time cannot be guaranteed. The design of the wax seal groove 106 in the present invention only requires the specimen tube to be inverted and the wax liquid to be dripped into the dropper. It is not only easy to operate and the wax seal layer is well protected in the groove, which can effectively extend the shelf life of the specimen and ensure the stability of the preservation environment. It can also conveniently realize the reversibility of the wax seal to achieve the reuse and flexible operation of the device. At the same time, the design of the buffer bin 202 not only avoids the volatilization of the preservation liquid in the preservation observation bin 201 to a great extent, but also facilitates timely maintenance or replacement through the sealing of the liquid level monitoring device in real time.

[0041] It is understandable that the sealing cover 1 with a rubber pad or rubber ring is a commonly used sealing structure that can prevent the volatilization of liquid. When it exists independently, it can avoid the volatilization of most liquids. However, the sealing structure is insufficient for the sealing of some highly volatile liquids, and will be affected by the aging of the rubber ring. The sealing effect is poor. During long-term storage, some highly volatile components in the preservation liquid will be reduced, thereby affecting the long-term preservation of the specimen; wax seal is a commonly used specimen sealing structure, which can prevent external pests, molds, etc. from entering the device to damage the specimen, and can also prevent the preservation liquid in the tube from volatilizing, playing a good isolation role; the previous wax sealing method is to directly drip near the tube mouth to form a cap-shaped wax structure, but the wax itself has a very low hardness and will fall off or be damaged by a slight scratch. The design of the wax seal groove 106 in the present invention can hide the wax structure on the inner side of the sealing cover 1, which plays a good protective role, so that when the specimen 2 is normally taken for observation, the wax seal structure will not be damaged. The present invention adopts a design method of combining a rubber ring sealing cover 1 and a wax sealing groove 106. The wax sealing groove 106 makes up for the insufficient sealing performance of the single sealing cover 1, and the sealing cover 1 protects the wax structure in the wax sealing groove 106 from being damaged. The two work together to improve the sealing effect of the immersion specimen, which is suitable for the long-term preservation of the immersion specimen.

[0042] In the actual operation of artificially encapsulating liquid-immersed specimens, it was found that it is almost impossible to have no bubbles in the test tube. This is because the rubber ring in the tube cap will retain air at the moment the tube mouth is covered. As a result, there must be some air in the tube, making it difficult to completely fill the entire test tube with liquid. Or as the storage time increases (because the specimens are different from other supplies and the storage time may be as long as decades, there may be aging of the rubber ring, falling off of the wax seal, etc.), air may enter. When observing the insect specimens in the test tube under a microscope, the test tube needs to be placed horizontally to observe the insect specimens in the tube. The bubbles formed by these air will float on the inside of the tube wall, blocking the line of sight.

[0043] The wall of the buffer chamber 202 is provided with a scale that can represent the depth of the specimen liquid in the chamber. The present invention sets a sealing component 3 in the connected buffer chamber 202 and the preservation observation chamber 201. It is not necessary to completely fill the buffer chamber 202 with the preservation liquid, but only needs to be kept above the safety scale. Air is allowed to exist in the buffer chamber 202. These air columns or bubbles cannot enter the preservation observation chamber 201 due to the obstruction of the sealing component 3, and cannot affect the observation. Therefore, through the arrangement of the specimen preservation chamber 2 and the sealing component 3 therein of the present invention, it is possible to effectively prevent bubbles from entering the preservation observation chamber 201, thereby ensuring the clarity of the observation field of view and preventing the influence of bubbles on the specimen position and morphology, and preventing the specimen 2 from floating and moving in the preservation solution, thereby reducing the difficulty of observation under a high-power microscope. In addition, it is also possible to determine whether the device is leaking based on the changes in the liquid level in the buffer chamber 202. Even if there is a leak, it will not directly affect the preservation observation chamber 201, thereby playing a role of buffering and prompting. When it is found that the liquid level in the buffer chamber 202 changes and a leak occurs, the tiny insect replacement device in the preservation observation chamber 201 can be replaced in time and resealed.

[0044] When observing immersion specimens, although cylindrical glass tubes, ampoules, etc. can be directly placed under a magnifying glass or microscope for observation, directly observing the specimens through the curved tube wall is prone to optical distortion, which affects the authenticity of the observation. The walls of the storage observation chamber 201 in the present invention are all flat, and the side walls of the flat surface are used as the observation surface, which can effectively reduce optical distortion and improve observation accuracy.

[0045] Preferably, the cross section of the storage observation bin 201 is triangular, rectangular or square. In this embodiment, the cross section of the storage observation bin 201 is square, and the storage observation bin 201 is a rectangular parallelepiped. The side length of the cross section of the rectangular storage observation bin 201 does not exceed 1 / 3 of the diameter of the cylindrical buffer bin 202, and the height of the storage observation bin 201 does not exceed 1 / 2 of the height of the buffer bin 202.

[0046] In order to make the storage and observation chamber 201 have the functions of storage and observation at the same time, the storage and observation chamber 201 is made of transparent material, which will cause the specimen 2 to be affected by the ambient light, and in serious cases, will cause the specimen to fade and the specimen liquid to degrade. Therefore, a light shielding protective cover 4 is provided on the outer cover of the storage and observation chamber 201. The light shielding protective cover 4 is a cylindrical shape that is open at the top and closed at the bottom. The outer diameter of the light shielding protective cover 4 is the same as the outer diameter of the buffer chamber 202. The height of the light shielding protective cover 4 is greater than the height of the storage and observation chamber 201 and is not less than 1 / 2 of the height of the buffer chamber 202. The lower outer wall of the buffer chamber 202 is provided with a first external thread 7, and the upper inner wall of the light shielding protective cover 4 is provided with a first internal thread used in conjunction with the first external thread 7, so that the light shielding protective cover 4 is arranged at the lower part of the buffer chamber 202 in a threaded connection. When it is necessary to observe the specimen in the storage and observation chamber 201, the light shielding protective cover 4 can be rotated to remove it from the buffer chamber 202.

[0047] Light can cause insect specimens to change color, fade, and even change their tissue and molecular structure. The design of the light-shielding protective cover 4 effectively reduces the impact of light on the specimens, and helps to maintain the stability of the specimens under different environmental conditions. In addition, the light-shielding protective cover 4 can protect the smaller storage and observation chamber 201 inside it. Compared with the cylindrical test tube, the storage and observation chamber 201 with a flat side wall has an irregular shape, especially the rectangular storage and observation chamber 201 has a slender structure, and the connection between adjacent side walls has edges, which is more susceptible to damage or wear, and there are hidden safety hazards. The light-shielding protective cover 4 has a more significant effect of protecting it. In addition, the irregular structure of large top and small bottom, round top and square bottom, there is no suitable specimen box or test tube rack on the market for unified storage. After installing the light-shielding protective cover 4, it not only provides additional physical protection for the storage observation chamber 201, but also forms a regular cylindrical shape together with the buffer chamber 202, so that the size of the entire device is consistent with the common 2ml cryogenic tubes on the market, and can be directly adapted to existing tube racks and specimen boxes, without the need to customize additional test tube racks or specimen boxes, thereby reducing the cost of device replacement and matching.

[0048] The combination of the light shielding protective cover 4 and the storage observation chamber 2 not only solves the influence of optical distortion on specimen observation and the influence of light irradiation on specimen preservation, but also makes up for the irregularity of the device structure and improves the protection of the specimen, making the device more suitable for standardized storage and specimen preservation. At the same time, the modular combination of the two also realizes the flexible switching of the specimen preservation function and the observation function, which significantly enhances the practicality and functionality of the device.

[0049] The storage observation device of the present invention is further described below in combination with its specific structure, size and usage method:

[0050] A device for preserving and observing micro-insect liquid-immersion specimens is provided, which comprises a sealing cover 1, a specimen preservation chamber 2, a sealing assembly 3 and a light-shielding protective cover 4. The overall size of the device is consistent with that of 2 ml cryotubes on the market, and can be adapted to the same tube rack and specimen box.

[0051] The sealing cover 1 is made of transparent hard plastic, with an inner diameter of 12 mm and a height of 8 mm. The cover has a threaded structure inside, which can be tightly screwed with the thread on the upper edge of the specimen storage chamber 2. A rubber gasket 104 is padded on the inner side of the cover top 101 to ensure that the liquid will not overflow after tightening. A raised fixing ring 105 is provided in the center of the sealing cover 1. The inner diameter of the fixing ring 105 is slightly larger than the rod diameter of the fixing rod 302, which is used to stabilize the fixing rod 302. In addition, the lower edge of the sealing cover 1 is designed as a wax sealing groove 106 with a diameter of 14 mm and a width of 2 mm, which is used to drip melted wax liquid to further enhance the sealing effect.

[0052] The specimen storage chamber 2 is made of high-transparency glass, with an upper cylindrical buffer chamber 202 with an outer diameter of 12 mm and a height of 25 mm, and a lower rectangular observation chamber with a length, width, and height of 4 mm and 12 mm. The upper and lower edges of the cylindrical buffer chamber 202 are threaded, the upper edge is screwed to the sealing cover 1, and the lower edge is screwed to the light-shielding protective cover 4. There are four protrusion structures evenly distributed on the lower edge of the inner wall of the buffer chamber 202, which are used to fix the position of the fixing rod 302 to ensure that the rubber stopper can completely cover the chamber opening of the storage observation chamber 201.

[0053] The upper part of the sealing component 3 is a fixed rod 302 made of hard plastic with a diameter of 3mm and a length of 22mm, and the lower part is installed with a cylindrical rubber plug with a height of 5mm and a diameter of 8mm.

[0054] The light-shielding protective cover 4 is a cylinder with an open end made of dark translucent glass, with an inner diameter of 12 mm and a height of 15 mm. The upper inner edge is threaded and can be tightly screwed to the lower edge of the cylindrical cache chamber of the specimen preservation chamber 2.

[0055] During assembly, first inject the preservation liquid into the specimen preservation chamber 2 so that the liquid level is slightly higher than the preservation observation chamber 201, then gently place the tiny insect specimen into the preservation observation chamber 201, and slightly adjust the position with tweezers; then slowly insert the sealing component 3, press the sealing plug 301 on the bottom of the buffer chamber 202 and cover the chamber opening of the rectangular preservation observation chamber 201, so that it forms a closed space with the preservation observation chamber 201, and at the same time ensure that no bubbles enter the preservation observation chamber 201, and then add preservation liquid to make the liquid level as close to the chamber opening of the specimen preservation chamber 2 as possible; then, align the fixed structure in the sealing cover 1 with the upper end of the fixing rod 302 and tighten it, turn the device upside down, drip an appropriate amount of melted wax liquid into the wax sealing groove 106, and form a second layer of sealing after the wax liquid solidifies; finally, screw the dark light-shielding protective cover 4 to the lower part of the cylindrical buffer chamber 202 to ensure that the specimen in the observation chamber is protected from light, and then it can be placed in a specimen box or specimen rack and put into a specimen cabinet for preservation. During the preservation process, the double sealing design effectively prevents the volatilization of the preservation solution, ensuring the long-term stable preservation of the specimen. At the same time, the light shielding protective cover 4 further reduces the influence of light on the color and tissue of the specimen. When observing, just gently unscrew the light shielding protective cover 4, lay the device flat and place the observation chamber in the center of the stage, and then you can use the stereo microscope to carefully observe and study the tiny insects and their local structural characteristics.

[0056] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for storing and observing micro-insect liquid-immersion specimens, comprising a specimen storage chamber (2) and a sealing cover (1), characterized in that: The specimen storage chamber (2) comprises a columnar storage observation chamber (201) and a columnar buffer chamber (202); the chamber opening of the storage observation chamber (201) is connected to the chamber bottom of the buffer chamber (202) to achieve communication between the storage observation chamber (201) and the buffer chamber (202); the cross-sectional area of ​​the storage observation chamber (201) is smaller than the cross-sectional area of ​​the buffer chamber (202); the storage observation chamber (201) and the buffer chamber (202) are both filled with storage liquid; and a sealing component (3) for sealing the chamber opening of the storage observation chamber (201) is provided in the buffer chamber (202).

2. The device for storing and observing micro-insect liquid immersion specimens according to claim 1, characterized in that: The sealing assembly (3) comprises a fixing rod (302) and a sealing plug (301) arranged at one end of the fixing rod (302); the end of the fixing rod (302) away from the sealing plug (301) can abut against the sealing cover (1).

3. The device for storing and observing micro-insect liquid immersion specimens according to claim 2, characterized in that: The cross-sections of the buffer bin (202) and the sealing plug (301) are both circular, the diameter of the sealing plug (301) is smaller than the inner diameter of the buffer bin (202), and the overall height of the sealing plug (301) and the fixing rod (302) is greater than the height of the buffer bin (202).

4. The device for storing and observing micro-insect liquid immersion specimens according to claim 3, characterized in that: The bottom of the buffer bin (202) is provided with a plurality of positioning protrusions (6) distributed at intervals in the circumferential direction, and the inner edges of the plurality of positioning protrusions (6) are in contact with the side surface of the sealing plug (301).

5. The device for storing and observing micro-insect liquid-immersion specimens according to claim 1, characterized in that: The side surface of the sealing cover (1) is provided with a threaded section (102) threadedly connected to the buffer bin (202) and a wax seal section (103) sleeved on the outside of the buffer bin (202) in sequence, the wax seal section (103) and the outer wall of the buffer bin (202) enclose a wax seal groove (106), and a rubber gasket (104) is provided on the inner side of the cover top (101) of the sealing cover (1).

6. The device for storing and observing micro-insect liquid immersion specimens according to claim 2, characterized in that: The fixing rod (302) is fixedly connected to the center position of the inner side of the cover top (101) of the sealing cover (1).

7. The device for storing and observing micro-insect liquid-immersion specimens according to claim 1, characterized in that: The walls of the storage and observation chamber (201) are all flat.

8. The device for storing and observing micro-insect liquid-immersion specimens according to claim 7, characterized in that: The cross section of the storage and observation chamber (201) is triangular, rectangular or square.

9. The device for storing and observing micro-insect liquid immersion specimens according to claim 1, characterized in that: The outer cover of the storage observation chamber (201) is provided with a light shielding protective cover (4).

10. The device for storing and observing micro-insect liquid immersion specimens according to claim 9, characterized in that: The buffer bin (202) and the light shielding protective cover (4) are both cylindrical and have the same outer diameter, and the light shielding protective cover (4) is threadedly connected to the lower part of the buffer bin (202).

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