Complex gel for treating skin aging by using PLLA, CMC and hyaluronic acid and preparation method thereof

Abstract

The invention relates to a composite gel for treating skin aging by using PLLA, CMC and hyaluronic acid. The composite gel is prepared from the following raw materials: 300 to 310 mg of PLLA microspheres for injection, 50 mg of CMC, 35 to 40 mg of hyaluronic acid and 5 to 8 ml of normal saline. The invention also relates to a preparation method of the composite gel for treating skin aging by using PLLA, CMC and hyaluronic acid. The PLLA microspheres and the hyaluronic acid are combined for use, respective advantages can be exerted, and the PLLA microspheres can provide lasting stimulation and promote an organism to continuously generate collagen; and hyaluronic acid can immediately improve the moisture degree and plumpness of the skin. The combination of the collagen and the collagen has the effects of integrating multiple effects, enhancing aging resistance and improving skin quality, and fundamentally solves the problem of skin aging caused by loss of collagen.

Description

Technical Field

[0001] The embodiments of the present invention relate to the field of medical cosmetology, and specifically to a composite gel for treating skin aging using PLLA, CMC, and hyaluronic acid and a preparation method thereof. Background Art

[0002] As one of the most abundant proteins in the human body, collagen plays a vital role in maintaining skin elasticity and firmness and repairing damaged cells. However, with the increase of age, the influence of various factors such as ultraviolet radiation, bad living habits and environmental pollution, the collagen in the human body will gradually be lost, resulting in aging phenomena such as sagging, wrinkles, dryness and dullness of the skin.

[0003] When collagen is lost, the skin's ability to retain moisture decreases, leading to dryness and fine lines. At the same time, the skin loses its supporting structure, gradually becomes loose and develops deep wrinkles. In addition, the reduction of collagen also affects the skin's pigment metabolism, leading to problems such as spots and uneven skin tone.

[0004] At present, the main treatments for skin aging caused by collagen loss include oral collagen supplements, topical anti-aging skin care products, and medical cosmetic methods such as collagen injections. However, these methods all have certain limitations. Although oral collagen can replenish collagen in the body, its absorption efficiency is limited; topical skin care products have difficulty penetrating the skin barrier to reach the dermis; and collagen injections have problems such as a short maintenance time.

[0005] Therefore, there is an urgent need to propose a composite gel that can have multiple effects in one and can treat skin aging caused by collagen loss. Summary of the invention

[0006] In view of the above problems, the present invention is proposed to provide a composite gel for treating skin aging using PLLA, CMC and hyaluronic acid and a preparation method thereof, which overcomes the above problems or at least partially solves the above problems.

[0007] In a first aspect, an embodiment of the present invention provides a composite gel for treating skin aging using PLLA, CMC, and hyaluronic acid, which is composed of the following raw materials: 300-310 mg of injectable PLLA microspheres, 50 mg of CMC, 35-40 mg of hyaluronic acid, and 5-8 ml of normal saline.

[0008] Preferably, the composite gel is composed of the following raw materials: 300 mg of PLLA microspheres for injection, 50 mg of CMC, 40 mg of hyaluronic acid, and 5 ml of normal saline.

[0009] Preferably, the particle size of the injectable PLLA microspheres ranges from 10 to 50 μm; the molecular weight of the PLLA ranges from 10 KDa to 11 KDa.

[0010] Preferably, the molecular weight of the hyaluronic acid is in the range of 3000 to 5000 Da.

[0011] In a second aspect, the embodiment of the present invention further provides a method for preparing the composite gel for treating skin aging using PLLA, CMC and hyaluronic acid as described in the first aspect, characterized in that it comprises the following steps:

[0012] S1. Divide the normal saline solution into two equal parts and place them in containers respectively;

[0013] S2, slowly add CMC and hyaluronic acid into two containers containing physiological saline respectively, stir thoroughly to dissolve, mix the physiological saline containing CMC and hyaluronic acid, continue stirring until uniform, and obtain mixture 1;

[0014] S3. Add the PLLA microspheres for injection into the mixture one, and use the three-way valve to repeatedly operate until the PLLA microspheres are evenly dispersed in the mixture one to obtain a composite gel.

[0015] In a third aspect, the embodiments of the present invention also provide the use of the composite gel for treating skin aging using PLLA, CMC and hyaluronic acid as described in the first aspect in treating skin aging problems caused by collagen loss.

[0016] The present invention combines PLLA microspheres with hyaluronic acid to give full play to their respective advantages. PLLA microspheres can provide lasting stimulation and promote the body to continuously produce collagen, while hyaluronic acid can immediately improve the moisture and fullness of the skin. The combination of the two has multiple effects in one, enhancing the effects of anti-aging and improving skin quality, and fundamentally solving the problem of skin aging caused by the loss of collagen. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The included drawings are used to provide a further understanding of the embodiments of the present application, which constitute a part of the specification, are used to illustrate the implementation methods of the present application, and together with the text description, explain the principles of the present application. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0018] Figure 1 The facial skin condition of the volunteers before the rolling needle treatment using the composite gel prepared in the embodiment of the present invention;

[0019] Figure 2The facial skin condition of volunteers after the first course of roller needle treatment using the composite gel prepared in an embodiment of the present invention;

[0020] Figure 3 The facial skin condition of volunteers after the second course of roller needle treatment using the composite gel prepared in an embodiment of the present invention;

[0021] Figure 4 The facial skin condition of a volunteer after the third course of roller needle treatment using the composite gel prepared by an embodiment of the present invention. DETAILED DESCRIPTION

[0022] In order to facilitate understanding by those skilled in the art, the present invention will be further described below with reference to embodiments.

[0023] In a first aspect, an embodiment of the present invention provides a composite gel for treating skin aging using PLLA, CMC, and hyaluronic acid, which is composed of the following raw materials: 300-310 mg of injectable PLLA microspheres, 50 mg of CMC, 35-40 mg of hyaluronic acid, and 5-8 ml of normal saline.

[0024] In one embodiment, the composite gel is composed of the following raw materials: 300 mg of PLLA microspheres for injection, 50 mg of CMC, 40 mg of hyaluronic acid, and 5 ml of normal saline.

[0025] In one embodiment, the particle size of the injectable PLLA microspheres ranges from 10 to 50 μm; the molecular weight of the PLLA ranges from 10 KDa to 11 KDa.

[0026] In one embodiment, the molecular weight of the hyaluronic acid ranges from 3000 to 5000 Da.

[0027] In a second aspect, the embodiment of the present invention further provides a method for preparing the composite gel for treating skin aging using PLLA, CMC and hyaluronic acid as described in the first aspect, characterized in that it comprises the following steps:

[0028] S1. Divide the normal saline solution into two equal parts and place them in containers respectively;

[0029] S2, slowly add CMC and hyaluronic acid into two containers containing physiological saline respectively, stir thoroughly to dissolve, mix the physiological saline containing CMC and hyaluronic acid, continue stirring until uniform, and obtain mixture 1;

[0030] S3. Add the PLLA microspheres for injection into the mixture one, and use the three-way valve to repeatedly operate until the PLLA microspheres are evenly dispersed in the mixture one to obtain a composite gel.

[0031] In a third aspect, the embodiments of the present invention also provide the use of the composite gel for treating skin aging using PLLA, CMC and hyaluronic acid as described in the first aspect in treating skin aging problems caused by collagen loss.

[0032] PLLA microspheres, or poly-L-lactic acid microspheres, are a type of biodegradable material widely used in the field of medical cosmetology. They promote collagen regeneration by stimulating fibroblasts in the skin. The principle of PLLA microspheres stimulating collagen regeneration mainly involves the following aspects:

[0033] Lactic acid signaling activity induction. During the gradual degradation of PLLA microspheres in the body, lactic acid is released. As a signaling molecule, lactic acid can induce fibroblasts and other cells to respond, thereby promoting the regeneration of collagen. This process is a manifestation of "active regeneration". Unlike simple "inflammatory stimulation", it is gentler and more effective.

[0034] Immune response and cell proliferation. When PLLA microspheres are implanted in the body, they are considered foreign bodies, which triggers an immune response. During this process, macrophages and foreign body giant cells are recruited to the implantation site and stimulate fibroblast proliferation. Under the action of growth factors such as TGFβ1 (transforming growth factor β1), fibroblasts are transformed into myofibroblasts, which are able to synthesize and secrete collagen.

[0035] Deposition and transformation of collagen. With the degradation of PLLA microspheres and the proliferation of cells, type I collagen and type III collagen will be deposited at the implantation site. After injection of PLLA microspheres, they can promote the regeneration of type III collagen and type I collagen at the same time, ultimately improving the collagen content and quality in the skin.

[0036] Synergistic and lasting effects. The process of PLLA microspheres stimulating collagen regeneration is a continuous and gradual process. With the gradual degradation of microspheres and the continuous deposition of collagen, the skin tissue will gradually be repaired and regenerated. This regeneration effect is not only reflected in the improvement of the skin surface (such as wrinkle reduction, skin quality improvement, etc.), but can also penetrate into the dermis of the skin, fundamentally solving the problem of skin aging.

[0037] CMC, namely carboxymethyl cellulose. It is a carboxymethylated derivative of cellulose and the most important ionic cellulose gum. CMC has the characteristics of high viscosity, strong suspension power, strong hygroscopicity, easy solubility in water, good stability, not easy to corrupt, harmless to human body, etc. In the present invention, CMC is used as a suspending agent to improve the texture and stability of the present invention.

[0038] The composite gel of the present invention can be used in conjunction with a rolling needle to open the skin channel, so that the effective ingredients in the composite gel can quickly penetrate into the dermis. The particle size range of the PLLA microspheres used in the present invention is 10 to 50 μm; after the PLLA microspheres enter the skin tissue or deep tissue in a uniform size, they can be evenly dispersed and gradually disintegrated and released in the skin tissue. It can not only promote the regeneration of collagen, but also this regeneration is gradual, avoiding adverse skin reactions caused by excessive stimulation, and can promote the body to continuously produce collagen, achieving the effect of treating skin aging.

[0039] The present invention combines PLLA microspheres with hyaluronic acid to give full play to their respective advantages. PLLA microspheres can provide lasting stimulation and promote the body to continuously produce collagen, while hyaluronic acid can immediately improve the moisture and fullness of the skin. The combination of the two has multiple effects in one, enhancing the effects of anti-aging and improving skin quality, and fundamentally solving the problem of skin aging caused by the loss of collagen.

[0040] The following is a further detailed description of a composite gel for treating skin aging using PLLA, CMC and hyaluronic acid and a preparation method thereof in conjunction with specific embodiments.

[0041] Example

[0042] A composite gel for treating skin aging using PLLA, CMC and hyaluronic acid, comprising the following raw materials: 300 mg of PLLA microspheres for injection, 50 mg of CMC, 40 mg of hyaluronic acid and 5 ml of normal saline. The particle size of the PLLA microspheres for injection ranges from 10 to 50 μm; the molecular weight of the PLLA ranges from 10 KDa to 11 KDa; and the molecular weight of the hyaluronic acid ranges from 3000 to 5000 Da.

[0043] The preparation method of the composite gel comprises the following steps:

[0044] S1. Divide the normal saline solution into two equal parts and place them in containers respectively;

[0045] S2, slowly add CMC and hyaluronic acid into two containers containing physiological saline respectively, stir thoroughly to dissolve, mix the physiological saline containing CMC and hyaluronic acid, continue stirring until uniform, and obtain mixture 1;

[0046] S3. Add the PLLA microspheres for injection into the mixture one, and use the three-way valve to repeatedly operate until the PLLA microspheres are evenly dispersed in the mixture one to obtain a composite gel.

[0047] Example Method of using the composite gel:

[0048] The facial skin of the test subject is cleaned and disinfected; after the roller needle is disinfected, it is rolled on the facial skin of the test subject to open the skin channel; during the roller needle operation, the composite gel prepared in the embodiment is continuously applied; when applying, it is necessary to ensure that the composite gel covers the entire facial skin of the test subject.

[0049] Effect test

[0050] The composite gel of the embodiment was used as a test sample, and one volunteer was recruited to participate in the test. The subject had aging problems such as sagging, wrinkles, dryness, and dullness of the skin.

[0051] Subject restrictions: During the trial, the test site should use the test samples provided in this trial, and no other products with equivalent efficacy or that may affect the test results should be used.

[0052] The test sample of the volunteers was the composite gel prepared in the embodiment, which was used in combination with rolling needle treatment for a total of three courses. After the first course, the second course was carried out after an interval of 15 days, and the third course was carried out after an interval of one month. After each treatment, a medical repair mask was applied on the face. On weekdays, basic moisturizing products were used and physical sun protection was performed. After each treatment, photos were taken for record. After the third course, the photos were compared ( Figure 1-Figure 4 ), evaluate the therapeutic effect.

[0053] from Figure 1 It can be seen that the volunteers had obvious aging problems such as loose skin, wrinkles, dryness, and dullness before the operation. Figure 2 ), dull skin improved, nasolabial folds, perioral wrinkles and eye bags were reduced; after the second course of treatment (see Figure 3 ), skin dullness further improved, nasolabial folds, perioral wrinkles and eye bags further lightened; after the third course of treatment (see Figure 4 ), skin complexion is visibly brightened, skin texture is visibly improved, nasolabial folds are visibly improved, and the jawline is visibly lifted.

[0054] The present invention combines PLLA microspheres with hyaluronic acid to give full play to their respective advantages. PLLA microspheres can provide lasting stimulation and promote the body to continuously produce collagen; while hyaluronic acid can immediately improve the moisture and plumpness of the skin. The combination of the two has multiple effects in one, enhancing the effects of anti-aging and improving skin quality, and fundamentally solving the problem of skin aging caused by the loss of collagen.

[0055] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A composite gel for treating skin aging using PLLA, CMC and hyaluronic acid, characterized in that: It is composed of the following raw materials: 300-310 mg of PLLA microspheres for injection, 50 mg of CMC, 35-40 mg of hyaluronic acid, and 5-8 ml of normal saline.

2. The composite gel for treating skin aging using PLLA, CMC and hyaluronic acid according to claim 1, characterized in that: It is composed of the following raw materials: 300 mg of PLLA microspheres for injection, 50 mg of CMC, 40 mg of hyaluronic acid, and 5 ml of normal saline.

3. The composite gel for treating skin aging using PLLA, CMC and hyaluronic acid according to claim 1, characterized in that: The particle size range of the PLLA microspheres for injection is 10 to 50 μm; the molecular weight range of the PLLA is 10 KDa to 11 KDa.

4. The composite gel for treating skin aging using PLLA, CMC and hyaluronic acid according to claim 1, characterized in that: The molecular weight range of the hyaluronic acid is 3000 to 5000 Da.

5. A method for preparing a composite gel for treating skin aging using PLLA, CMC and hyaluronic acid as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Divide the normal saline solution into two equal parts and place them in containers respectively; S2, slowly add CMC and hyaluronic acid into two containers containing physiological saline respectively, stir thoroughly to dissolve, mix the physiological saline containing CMC and hyaluronic acid, continue stirring until uniform, and obtain mixture 1; S3. Add the PLLA microspheres for injection into the mixture one, and use the three-way valve to repeatedly operate until the PLLA microspheres are evenly dispersed in the mixture one to obtain a composite gel.

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