Root length damage-free rapid detection and salt tolerance identification device in rice seedling stage

Abstract

The invention relates to the technical field of rice salt tolerance identification, and discloses a rice seedling stage root length damage-free rapid detection salt tolerance identification device which comprises an identification pool, the front face of the identification pool is a transparent observation window, a culture floating plate is movably placed in the middle of the identification pool, and culture openings are evenly formed in the middle of the culture floating plate; a culture net is fixedly installed at the bottom of the culture opening, supporting bases are fixedly connected to the two sides of the bottom of the identification pool, and moving rails are fixedly connected to the front faces and the back faces of the middles of the supporting bases. The movable supporting plate is additionally arranged at the bottom of the identification pool, and the rotor in the identification pool is driven by the rotating shaft magnetic strip on the movable supporting plate to rotate to form a water vortex, so that a downward pulling force is formed on the center line of the vortex, and rice roots at the bottom of a culture opening are conveniently gathered and stretched; therefore, experimenters can visually observe the growth length of the root system of the rice seedling, and tedious operation of measuring the root length and damage to the root system of the rice seedling are avoided.

Description

Technical Field

[0001] The invention relates to the technical field of rice salt tolerance identification, in particular to a device for rapid detection of rice salt tolerance identification without damage to root length at the rice seedling stage. Background Art

[0002] Rice salt tolerance detection technology refers to a series of technologies and methods used to evaluate the tolerance of rice varieties or germplasm to salt stress. These technologies are designed to help agricultural researchers and breeders screen out rice varieties that are more adaptable to saline-alkali environments in order to improve rice yield and quality. Existing rice salt tolerance detection is usually based on the final growth status of rice for evaluation, ignoring the growth indicators of rice during the rice seedling growth process. The growth indicators of rice in the seedling stage can better reflect the salt tolerance of rice, and the characteristic that best reflects the rice growth indicators is the root length of rice.

[0003] In the salt tolerance identification experiment of rice seedlings, the detection method mostly used is to pull out the test rice seedlings, cut the roots and measure their growth. This method will cause irreversible damage to the test rice, making it difficult to detect the growth indicators of the rice in the next growth cycle, and thus difficult to obtain continuous root growth data of the rice seedlings in multiple time periods; secondly, during the long-term cultivation process of the culture solution in the incubator, the nutrients in the salt pool and the supplemented brine will precipitate unevenly, affecting the accuracy of the test data. Summary of the invention

[0004] The invention aims at the shortcomings of the existing rice seedling salt tolerance test and identification device during use proposed in the background technology.

[0005] The present invention provides the following technical solution: a device for rapid detection and salt tolerance identification of root length without damage in the seedling stage of rice, comprising an identification pool, the front of the identification pool is a transparent observation window, a culture float is movably placed in the middle of the identification pool, culture ports are evenly opened in the middle of the culture float, a culture net is fixedly installed at the bottom of the culture port, two sides of the bottom of the identification pool are fixedly connected with a support base, the front and back sides of the middle of the support base are fixedly connected with a moving track, a slide groove is opened in the middle of the moving track, and a moving support plate is movably connected through the slide groove, the slide groove on the moving track on one side of the front of the support base runs through the moving track on this side, and one end of the front side of the moving support plate passes through the slide groove The movable track extends to the outside of the movable track, and a movable dial button is fixedly connected to the front of the movable support plate, and the slide groove on the movable track on the back side of the support base is located on the inner side of the movable track and does not penetrate through it, and the slide groove is provided with a positioning hole at the position corresponding to the center of the culture port, and a motor is fixedly installed on the top of the movable support plate, and a shaft magnetic strip is fixedly connected to the rotating shaft of the motor in the horizontal direction, and a rotor is movably placed on the bottom surface of the identification pool, and a main rotor is fixedly connected to the bottom of the culture float plate, and a return water groove is provided at the connection between the top of the main rotor and the bottom surface of the culture float plate, and the internal movably sleeve of the main rotor is connected with a first secondary rotor, and the internal movably sleeve of the first secondary rotor is connected with a second secondary rotor.

[0006] Preferably, the culture float rises along with the liquid level in the identification pool, ensuring that the culture net is always in contact with the liquid surface in the identification pool.

[0007] Preferably, a raised clamping shaft is fixedly connected to the back side of the movable support plate, and the diameter of the clamping shaft is smaller than the inner diameter of the positioning hole, and the opening position of the positioning hole is consistent with the opening position of the culture port.

[0008] Preferably, the central axis of the main rotor is consistent with the central axis of the culture port.

[0009] Preferably, the first secondary rotor can move up and down along the main rotor, and a limit ring is provided on the top of the first secondary rotor to prevent the first secondary rotor from escaping from the inside of the main rotor. The second secondary rotor can move up and down along the first secondary rotor, and a limit ring is provided on the top of the second secondary rotor to prevent the second secondary rotor from escaping from the inside of the first secondary rotor.

[0010] Preferably, the inner walls of the main rotor and the first secondary rotor are provided with threaded grooves, the first secondary rotor and the second secondary rotor are provided with rotation shafts on both sides of the top, and the rotation shaft at the top of the second secondary rotor is movably engaged with the threaded groove on the inner wall of the first secondary rotor, the rotation shaft at the top of the first secondary rotor is movably engaged with the threaded groove on the inner wall of the main rotor, a coil spring is fixedly installed in the middle of the main rotor and the first secondary rotor, a coil spring is fixedly installed in the middle of the first secondary rotor and the second secondary rotor, and a fan blade is fixedly installed at the bottom in the middle of the second secondary rotor.

[0011] Preferably, the coil spring pulls up the first auxiliary rotor and contracts the second auxiliary rotor into the main rotor when it is not under force.

[0012] The present invention has the following beneficial effects: 1. The present invention installs a movable support plate at the bottom of the identification pool, and uses the rotating shaft magnetic strip on the movable support plate to drive the rotor in the identification pool to rotate to form a water vortex, thereby forming a downward pulling force on the center line of the vortex, so as to facilitate the gathering and stretching of the rice roots at the bottom of the culture port, so that the experimenter can intuitively observe the growth length of the rice seedling root system without removing the seedling with its roots from the culture floating plate for measurement, thereby avoiding the tedious operation of measuring the root length and the damage to the rice seedling root system.

[0013] 2. The present invention provides a main vortex coaxial with the culture port under the culture float plate, and uses the main vortex to form a circle for the water vortex, so as to avoid the roots under the culture port of an adjacent group being drawn into and pulled to cause root damage when measuring the root length under a certain group of culture ports, thereby further ensuring the protection of the root system during the measurement process, so that the roots of the rice seedlings after measurement are not affected, thereby easily obtaining continuous root growth data of the rice seedlings in multiple time periods.

[0014] 3. The present invention avoids the problem of the culture float being lifted up by the main rotor when the water level in the identification pool is at the bottom, causing the culture net to be out of the water. At the same time, the coil spring and the threaded groove in the three-stage rotor are used to make the first auxiliary rotor and the second auxiliary rotor shrink into the main rotor in a normal state, which will not restrict and affect the free growth of the rice seedling root system.

[0015] 4. The present invention arranges the water vortex formed by the main vortex and the rotor to cooperate with each other, so that after the culture solution in the main vortex generates a downward water flow along the center line, the culture solution is thrown out from the bottom of the second sub-vortex, and at the same time, the culture solution is replenished inward from the return water tank above the main vortex, thereby performing a large mixing of the culture solution in the identification pool at the upper and lower levels, avoiding the uneven precipitation of nutrients in the salt pool and the supplemented brine during the long-term cultivation process, which affects the accuracy of the test data. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a cross-sectional schematic diagram of the present invention without a coil spring; Figure 2 It is a schematic diagram of the four-section section of the overall structure of the present invention; Figure 3 It is a vertical cross-sectional schematic diagram of the overall structure of the present invention.

[0017] In the figure: 1. identification pool; 2. culture float; 201. culture port; 202. culture net; 3. support base; 4. moving track; 401. positioning hole; 5. moving support plate; 501. moving dial; 6. motor; 601. rotating shaft magnetic strip; 602. rotor; 7. main rotor; 701. first sub-rotor; 702. second sub-rotor; 703. fan blade; 8. coil spring; 801. threaded groove. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-2A device for rapid detection of salt tolerance of rice seedling root length without damage comprises an identification pool 1, the front of the identification pool 1 is a transparent observation window, a culture float 2 is movably placed in the middle of the identification pool 1, a culture port 201 is evenly opened in the middle of the culture float 2, a culture net 202 is fixedly installed at the bottom of the culture port 201, the culture float 2 rises and floats with the liquid level in the identification pool 1, ensuring that the culture net 202 is always in contact with the liquid level in the identification pool 1, and a support base 3 is fixedly connected to both sides of the bottom of the identification pool 1, and the front and back sides of the middle of the support base 3 are fixedly connected. A movable track 4 is provided in the middle of the movable track 4, and a movable support plate 5 is movably connected with the movable track 4 through the movable track. The movable track 4 on the front side of the support base 3 passes through the movable track 4 on this side, and one end of the front side of the movable support plate 5 passes through the movable track 4 to the outside of the movable track 4 through the movable track. A movable dial button 501 is fixedly connected to the front side of the movable support plate 5. The movable track 4 on the back side of the support base 3 is located on the inner side of the movable track 4 and does not pass through. The movable track is provided with a positioning hole 401 at the position corresponding to the center of the culture port 201. The movable support plate 5 The back of the movable support plate 5 is fixedly connected with a protruding card shaft, and the diameter of the card shaft is smaller than the inner diameter of the positioning hole 401, so that when the movable support plate 5 moves along the slide groove to the positioning hole 401, the movable dial button 501 is pushed to make the protruding card shaft embed into the positioning hole 401 to ensure that the position of the movable support plate 5 is fixed, and the opening position of the positioning hole 401 is consistent with the opening position of the culture port 201, so that the fixed position of the movable support plate 5 is directly below the culture port 201, and the top of the movable support plate 5 is fixedly installed with a motor 6, and the rotating shaft of the motor 6 is fixedly connected with a rotating shaft magnetic strip 60 in the horizontal direction. 1. A rotor 602 is movably placed on the bottom surface of the identification pool 1. The rotor 602 adsorbed by the rotating shaft magnetic strip 601 can rotate and move with the motor 6, so that a water vortex is formed at the bottom of the culture port 201 as the rotation center, and a downward pulling force is formed on the center line of the vortex, so that the rice roots at the bottom of the culture port 201 are gathered and stretched, so that the experimenter can intuitively observe the growth length of the rice seedling root system without removing the seedling with its roots from the culture floating plate 2 for measurement, thereby avoiding the tedious operation of measuring the root length and the damage to the rice seedling root system.

[0020] See also Figure 2The bottom of the culture floating plate 2 is fixedly connected with a main rotor 7, and the central axis of the main rotor 7 is consistent with the central axis of the culture port 201. A return water groove is provided at the connection between the top of the main rotor 7 and the bottom surface of the culture floating plate 2. The main rotor 7 forms a water vortex to prevent the roots under the culture port 201 of the adjacent group from being drawn into the roots when measuring the root length under a certain group of culture ports 201, and pulling the roots to cause root damage. The first auxiliary rotor 701 is movably sleeved inside the main rotor 7. The first auxiliary rotor 701 can move up and down along the main rotor 7. A limiting ring is provided on the top of the first auxiliary rotor 701 to prevent the first auxiliary rotor 701 from separating from the main rotor 7 Internally, the first sub-rotor 701 has a second sub-rotor 702 movably sleeved therein, and the second sub-rotor 702 can move up and down along the first sub-rotor 701. A limit ring is arranged on the top of the second sub-rotor 702 to prevent the second sub-rotor 702 from being separated from the interior of the first sub-rotor 701. The three-stage rotor arrangement avoids the length of the single main rotor 7 from being fixed, limits the floating height of the culture float 2, and prevents the culture float 2 from being lifted up by the main rotor 7 when the water level in the identification pool 1 is at the bottom, causing the culture net 202 to be separated from the water surface. The main rotor 7, the first sub-rotor 701 and the second sub-rotor 702 are all made of transparent materials.

[0021] See also Figure 3 , the inner walls of the main rotor 7 and the first sub-rotor 701 are provided with a thread groove 801, and the first sub-rotor 701 and the second sub-rotor 702 are provided with a rotation shaft on both sides of the top, and the rotation shaft at the top of the second sub-rotor 702 is movably engaged with the thread groove 801 on the inner wall of the first sub-rotor 701, and the rotation shaft at the top of the first sub-rotor 701 is movably engaged with the thread groove 801 on the inner wall of the main rotor 7, so as to ensure that the second sub-rotor 702 and the first sub-rotor 701 can be respectively rotated and raised to the first sub-rotor 701 and the main rotor 7 along the thread groove 801, and a coil spring 8 is fixedly installed in the middle of the main rotor 7 and the first sub-rotor 701, and a coil spring 8 is fixedly installed in the middle of the first sub-rotor 701 and the second sub-rotor 702, and the coil spring 8 is not subjected to force When the root length of the rice seedlings needs to be measured, the movable support plate 5 drives the rotor 602 to rotate to form a water vortex under the main rotor 7. The water vortex drives the fan blades 703 to make the second auxiliary rotor 702 rotate along the thread groove 801, thereby overcoming the elastic force of the coil spring 8 and rotating the first auxiliary rotor 701 and the second auxiliary rotor 702 downward out of the main rotor 7, ensuring the stability of the central axis of the water vortex during measurement while isolating the rice seedlings on both sides to avoid damage to them.

[0022] The working principle of the method of the present invention is as follows: When in use, the identification pool 1 is injected with a rice culture solution of the salinity required for the salt tolerance identification test, and the culture float 2 is placed to float on the culture solution. The rice seeds to be identified are placed on the culture net 202 in the culture port 201 for culture. When the growth length of the root system needs to be measured, the movable dial button 501 is slid to move the movable support plate 5 to the bottom of the culture port 201 where the seeds to be detected grow, and the movable dial button 501 is pushed inward so that the protruding clamping shaft on the back of the movable support plate 5 is embedded in the positioning hole 401 at the location. , thereby fixing the position of the motor 6 so that the center line of the water vortex generated when the subsequent rotor 602 rotates is consistent with the center line position of the culture port 201. At this time, the power supply of the motor 6 is turned on, and the rotation of the shaft magnetic strip 601 drives the rotor 602 to rotate. The generated water vortex drives the fan blade 703 to make the second auxiliary rotor 702 rotate along the thread groove 801, thereby overcoming the elastic force of the coil spring 8 to rotate the first auxiliary rotor 701 and the second auxiliary rotor 702 downward from the main rotor 7, and the culture liquid in the main rotor 7 generates a downward flow along the center line. After the water flows, it is thrown outward from the bottom of the second auxiliary drum 702 by the rotor 602, and at the same time, the culture solution is added into the return water tank above the main drum 7, forming a water flow pulling force from top to bottom in the main drum 7 to pull the root system in the main drum 7 downward and straighten it, so that the experimenter can intuitively observe the growth length of the rice seedling root system without removing the seedling with its roots from the culture floating plate 2 for measurement, avoiding the tedious operation of measuring the root length and causing damage to the rice seedling root system. After the measurement and recording are completed, the power supply of the motor 6 is turned off, and the rotor 602 stops When the main rotor 701 is rotated, the fan blade 703 which is no longer moved by the water vortex is no longer under force. At this time, the first auxiliary rotor 701 and the second auxiliary rotor 702 are pulled up and retracted into the main rotor 7 under the elastic force of the coil spring, and the free growth of the roots of the subsequent rice seedlings is not restricted. When the length of the root system under other culture ports 201 needs to be measured, the movable dial button 501 is pulled back to make the raised clamping shaft on the back of the movable support plate 5 disengage from the positioning hole 401 at that location, and then the movable dial button 501 is moved to the location to be measured again, and the above measurement method is repeated to perform rapid and non-destructive root length detection.

[0023] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0024] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for rapid detection of salt tolerance of rice seedlings without damage to root length, comprising an identification pool (1), characterized in that: The front of the identification pool (1) is a transparent observation window. A culture float (2) is movably placed in the middle of the identification pool (1). Culture ports (201) are evenly arranged in the middle of the culture float (2). A culture net (202) is fixedly installed at the bottom of the culture port (201). Support bases (3) are fixedly connected to both sides of the bottom of the identification pool (1). The front and back sides of the middle of the support base (3) are fixedly connected to movable tracks (4). A slide groove is arranged in the middle of the movable track (4), and a movable support plate (5) is movably connected to the slide groove. The slide groove on the movable track (4) on one side of the front of the support base (3) penetrates the movable track (4) on that side. One end of the front side of the movable support plate (5) passes through the movable track (4) to the outside of the movable track (4) through the slide groove. The front side of the movable support plate (5) is fixedly connected to the movable track (4). A moving dial button (501) is connected, the slide groove on the moving track (4) on the back side of the support base (3) is located on the inner side of the moving track (4) and does not penetrate through the slide groove, and a positioning hole (401) is opened at the position corresponding to the center of the culture port (201), a motor (6) is fixedly installed on the top of the moving support plate (5), and a rotating shaft magnetic strip (601) is fixedly connected to the rotating shaft of the motor (6) in the horizontal direction, and a rotor (602) is movably placed on the bottom surface of the identification pool (1), and the bottom of the culture float (2) is fixedly connected to the main rotor (7), and a return water groove is opened at the connection between the top of the main rotor (7) and the bottom surface of the culture float (2), and the inside of the main rotor (7) is movably sleeved with a first auxiliary rotor (701), and the inside of the first auxiliary rotor (701) is movably sleeved with a second auxiliary rotor (702).

2. The device for rapid detection and identification of salt tolerance of rice seedling root length without damage according to claim 1, characterized in that: The culture float (2) rises with the height of the liquid surface in the identification pool (1), ensuring that the culture net (202) is always in contact with the liquid surface in the identification pool (1).

3. The device for rapid detection and identification of salt tolerance of rice seedling root length without damage according to claim 1, characterized in that: A protruding clamping shaft is fixedly connected to the back of the movable support plate (5), and the diameter of the clamping shaft is smaller than the inner diameter of the positioning hole (401), and the opening position of the positioning hole (401) is consistent with the opening position of the culture port (201).

4. The device for rapid detection and identification of salt tolerance of rice seedling root length without damage according to claim 1, characterized in that: The central axis of the main rotor (7) is consistent with the central axis of the culture port (201).

5. The device for rapid detection and identification of salt tolerance of rice seedling root length without damage according to claim 1, characterized in that: The first auxiliary rotor (701) can move up and down along the main rotor (7), and a limit ring is provided on the top of the first auxiliary rotor (701) to prevent the first auxiliary rotor (701) from being separated from the inside of the main rotor (7); the second auxiliary rotor (702) can move up and down along the first auxiliary rotor (701), and a limit ring is provided on the top of the second auxiliary rotor (702) to prevent the second auxiliary rotor (702) from being separated from the inside of the first auxiliary rotor (701).

6. The device for rapid detection and identification of salt tolerance of rice seedling root length without damage according to claim 1, characterized in that: The inner walls of the main rotor (7) and the first auxiliary rotor (701) are provided with thread grooves (801); the tops of the first auxiliary rotor (701) and the second auxiliary rotor (702) are provided with rotation shafts on both sides; the rotation shaft at the top of the second auxiliary rotor (702) is movably engaged with the thread groove (801) on the inner wall of the first auxiliary rotor (701); the rotation shaft at the top of the first auxiliary rotor (701) is movably engaged with the thread groove (801) on the inner wall of the main rotor (7); a coil spring (8) is fixedly installed between the main rotor (7) and the first auxiliary rotor (701); a coil spring (8) is fixedly installed between the first auxiliary rotor (701) and the second auxiliary rotor (702); and a fan blade (703) is fixedly installed at the bottom of the middle of the second auxiliary rotor (702).

7. The device for rapid detection and identification of salt tolerance of rice seedling root length without damage according to claim 6, characterized in that: The coil spring (8) pulls up the first auxiliary rotor (701) and the second auxiliary rotor (702) in a state without force, and shrinks them into the main rotor (7).

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