Antibacterial composition and application thereof in preparation of external preparation for preventing and treating body odor

Abstract

The invention relates to the technical field of antibacterial materials, in particular to an antibacterial composition and application thereof in preparation of an external preparation for preventing and treating body odor. The antibacterial composition disclosed by the invention is prepared from radix sophorae flavescentis alkaloid and plant enzyme. According to the antibacterial composition with the sophora flavescens alkaloid and the plant enzyme as the main components, antibacterial experiment results show that when the sophora flavescens alkaloid and the plant enzyme are combined, the synergistic antibacterial effect can be achieved, the antibacterial composition has better antibacterial activity compared with single sophora flavescens alkaloid or plant enzyme of the same dosage, durability is not prone to being generated, and the curative effect is better. The sophora flavescens alkaloid and the plant enzyme are prepared into spray, lotion, tincture, ointment, gel and other external preparations, and the external preparations are convenient to use and high in practicability.

Description

Technical Field

[0001] The present invention relates to the technical field of antibacterial materials, in particular to an antibacterial composition and application thereof in preparing an external preparation for preventing and treating body odor. Background Art

[0002] Since the sweat glands of patients secrete a large amount of oil, which is easily decomposed into volatile unsaturated fatty acids by staphylococci, body odor will be generated. In addition, a very small number of patients with skin infection of Candida albicans may also produce body odor. Existing topical preparations for treating body odor commonly include antiperspirants, antibacterial agents, deodorants and fragrances, the purpose of which is to inhibit or reduce the production of human sweat, inhibit or eliminate the bacteria involved in the formation of body odor, absorb or decompose the odor substances that have been produced, and use fragrances to cover up the odor.

[0003] At present, the conventional preparations for treating body odor are basically chemical substances, such as aluminum oxide, aluminum chloride, and allantoin base in antiperspirants, which are chemical substances with strong astringent effects; methenamine solution (Xishilan Xialu), formaldehyde solution, trichlorodiphenylurea, pyrithione copper zinc and other drugs in antibacterial agents, which are chemical substances that can play an antibacterial role; zinc oxide nylon powder commonly used in deodorants is a chemical substance that can improve the unpleasant odor in wrinkled areas such as armpits; fragrances are physical methods that achieve the effect of enhancing fragrance and removing odor by continuously emitting fragrance, which only treats the symptoms but not the root cause. The external preparations of the above chemical substances generally have greater side effects and more allergic reactions.

[0004] Therefore, it is necessary to develop substances with the ability to prevent and treat body odor from natural medicines based on the existing technology. Summary of the invention

[0005] The purpose of the present invention is to address the above-mentioned deficiencies in the prior art and to provide an antibacterial composition and its use in the preparation of an external preparation for preventing and treating body odor.

[0006] The invention discloses an antibacterial composition comprising sophora flavescens alkaloids and plant enzymes.

[0007] Furthermore, the mass volume ratio of Sophora flavescens alkaloids and plant enzymes is 1-3:10-30 g / ml.

[0008] Furthermore, the matrine alkaloids include one or more of matrine, oxymatrine, hydroxymatrine, n-methyl cytisine, anagerine, bapeline and dehydromatrine.

[0009] Furthermore, the matrine alkaloids include matrine and oxymatrine.

[0010] Furthermore, the Sophora flavescens alkaloids are prepared by the following method: the Sophora flavescens medicinal material is crushed and passed through a 40-mesh sieve, the extraction solvent is 60% ethanol, the solid-liquid ratio is 1:8-12, reflux extraction is performed 3 times, each time for 30 minutes, the extraction temperature is 40°C, the extract is centrifuged at 4500r / min for 15 minutes to obtain a supernatant; the supernatant is filtered under reduced pressure, the filtrate is taken, and rotary evaporation is performed until there is no ethanol taste, an appropriate amount of concentrated ammonia test solution is added to alkalize to pH=9-10, and then chloroform is used for multiple extractions at a ratio of 1:1 until there is no color, the chloroform layers are mixed, the solvent is recovered to a dry paste, and it is adsorbed and purified by a macroporous resin of model HPD-100, and the purified analytical solution is concentrated under reduced pressure to remove ethanol and then freeze-dried.

[0011] Furthermore, the bacteria include Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans.

[0012] A use of the antibacterial composition as described above in the preparation of an external preparation for preventing and treating body odor.

[0013] Furthermore, the external preparation is a spray, a lotion, a tincture, an ointment, or a gel.

[0014] The antibacterial composition of the present invention has sophora flavescens alkaloids and plant enzymes as main components. The antibacterial experimental results show that the combination of sophora flavescens alkaloids and plant enzymes can play a synergistic antibacterial role, has better antibacterial activity than the same dose of single sophora flavescens alkaloids or plant enzymes, is not easy to produce durability, and has better therapeutic effect.

[0015] The invention prepares the sophora flavescens alkaloids and the plant enzyme into external preparations such as sprays, lotions, tinctures, ointments, gels, etc., which are convenient to use and highly practical. DETAILED DESCRIPTION

[0016] The following are specific embodiments of the present invention, which further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

[0017] Materials and Methods

[0018] Sophora flavescens alkaloids are prepared by the following method: Sophora flavescens medicinal materials are crushed and passed through a 40-mesh sieve, the extraction solvent is 60% ethanol, the solid-liquid ratio is 1:8, reflux extraction is performed 3 times, each time for 30 minutes, the extraction temperature is 40°C, and the extract is centrifuged at 4500r / min for 15 minutes to obtain a supernatant. The supernatant is filtered under reduced pressure, the filtrate is taken, and rotary evaporation is performed until there is no ethanol taste, and an appropriate amount of concentrated ammonia test solution is added to alkalize to pH=9-10, and then chloroform is used for multiple extractions at 1:1 until there is no color, the chloroform layers are mixed, and the solvent is recovered to a dry paste. The macroporous resin of HPD-100 model is used for adsorption and purification, and the purified analytical solution is concentrated under reduced pressure to remove ethanol and then freeze-dried to obtain it. The Sophora flavescens alkaloids are determined by high performance liquid chromatography, including matrine, oxymatrine, hydroxymatrine, n-methyl cytisine, anagerine, bapo leaf alkaloids and dehydromatrine.

[0019] Plant enzyme (Hubei Daoji Lingkang Enzyme Co., Ltd., batch number: 20240703), sodium chloride (Tianjin Guangfu Technology Development Co., Ltd., batch number: 20231205); chlorhexidine acetate (Jinzhou Jiutai Pharmaceutical Co., Ltd., batch number: 210701).

[0020] Bacterial strains and culture medium Standard strains Staphylococcus aureus [CMCC(B)26003], Staphylococcus epidermidis [CMCC(B)26069], and Candida albicans [CMCC(F)98001] were purchased from Wenzhou Weiqiong Inspection Technology Service Co., Ltd.; MH agar medium (batch number: 2306202), Sabouraud dextrose agar medium (2311222), nutrient agar medium (batch number: 2207202), and modified Martin agar medium (batch number: 220378) were purchased from Beijing Sanyao Technology Development Co., Ltd.

[0021] Instruments: water-proof constant temperature incubator (BG-270, Shanghai Boxun Industrial Co., Ltd.); biochemical incubator (BSP-100, Shanghai Boxun Medical Biological Instrument Co., Ltd.); biological safety cabinet (BSC-1100ⅡA2-X, Jinan Xinbeisi Biotechnology Co., Ltd.); vertical sterilizer (L100EP, Shandong Xinhua Medical Instrument Co., Ltd.); constant temperature drying oven (DHG model, Shaoxing Shangyu YiBo Experimental Instrument Co., Ltd.).

[0022] Example 1

[0023] The antibacterial composition comprises 1g of sophora flavescens alkaloids and 10ml of plant enzyme.

[0024] Example 2

[0025] The antibacterial composition comprises 1g of sophora flavescens alkaloids and 15ml of plant enzyme.

[0026] Example 3

[0027] The antibacterial composition comprises 1g of sophora flavescens alkaloids and 30ml of plant enzyme.

[0028] Example 4

[0029] The antibacterial composition comprises 2g of sophora flavescens alkaloids and 10ml of plant enzyme.

[0030] Example 5

[0031] The antibacterial composition comprises 3g of sophora flavescens alkaloids and 10ml of plant enzyme.

[0032] Antibacterial activity test

[0033] Disk Diffusion

[0034] Agar plate preparation

[0035] In the biosafety cabinet, immediately and quickly pour in the MH agar medium and Sabouraud glucose agar medium (agar thickness 3-4mm) that have been sterilized and cooled to about 45°C, and cover the plate lid. After the agar solidifies, mark them separately for later use. Among them, MH agar medium is used for the determination of the antibacterial effect of Staphylococcus aureus and Staphylococcus epidermidis, and Sabouraud glucose agar medium is used for the determination of the antibacterial effect of Candida albicans.

[0036] Preparation of bacterial solution: According to the aseptic operation method, a small amount of Staphylococcus aureus and Staphylococcus epidermidis strains were picked up with an inoculation loop and inoculated into nutrient agar slant medium, and then streaked and incubated in a 37℃ incubator for 24 hours. A small amount of Candida albicans strains were picked up with an inoculation loop and inoculated into modified Martin slant medium, and then streaked and incubated at 25℃ for 48 hours. A small amount of bacterial colonies were picked up and placed in a sterilized 0.9% sodium chloride solution test tube, and diluted to make the turbidity the same as that of a 0.5 McFarland turbidimetric tube (1.5×108CFU·mL-1).

[0037] Preparation of drug-sensitive paper sheets Select a circular filter paper sheet with a thickness of 0.7 mm and a diameter of 6 mm, place it in a dry and clean test tube, sterilize it at 121°C for 20 minutes, and then put it in a constant temperature drying oven to dry and take it out. Dilute the sample to saturated concentration with sterile water as the test sample. Take a number of filter paper sheets with sterilized tweezers and immerse them in the drug solution of the Sophora flavescens group, 0.9% sodium chloride solution and chlorhexidine acetate (0.5g / L) solution respectively. After the drug solution is completely absorbed by the paper sheet, gently dip it to remove the excess drug solution and set it aside.

[0038] Determination of in vitro antibacterial activity and method for judging experimental results

[0039] Determination of in vitro antibacterial activity: In a clean bench, use a sterile cotton swab to dip the bacterial solution, rotate along the inner wall of the tube to squeeze out the excess bacterial solution, evenly apply and inoculate on the surface of the MH agar plate 3 times, rotate the plate 60° to make the bacterial solution evenly distributed, and finally apply 1 circle along the inner wall of the plate. Let it stand for 5 minutes, use sterile tweezers to stick the drug-containing filter paper flat on the surface of the plate culture solution and mark the name of the drug solution on the outside of the plate, cover the plate cover, invert the culture, and use chlorhexidine acetate solution as a positive control and 0.9% sodium chloride solution as a negative control. Repeat 3 times for each group of drug solutions, observe the growth of bacteria and determine the diameter of the inhibition zone. Among them, MH agar medium is placed at 35℃ constant temperature for 24 hours, and Sabouraud glucose agar medium is placed at 25℃ for 48 hours.

[0040] Experimental result determination method

[0041] The diameter of the inhibition zone is determined by the cross method. The strength of the antibacterial ability can be expressed by the diameter of the inhibition zone. The inhibition zone result judgment standard in the "Technical Specifications for Disinfection" is adopted: ≤10mm is low sensitivity; 10-14 is moderate sensitivity; 15-20 is high sensitivity; ≥20mm is extremely sensitive.

[0042] The experimental results are shown in Table 1 below:

[0043] Table 1 Bacterial inhibition test results

[0044]

[0045]

[0046] The experimental results in Table 1 above show that the antibacterial composition provided by the present invention has a good antibacterial effect on a variety of pathogenic bacteria, especially after the matrine alkaloids and plant enzymes are combined, they can play a significant synergistic role, and have a better antibacterial effect than the same dose of matrine alkaloids and plant enzymes. In particular, the antibacterial effect is better when the matrine alkaloids and plant enzymes are 1g:30ml.

[0047] Effect Example

[0048] 30 patients with body odor were selected, including 20 males and 10 females, aged 12 to 60 years old, with an average age of 32 years old; the composition 3 prepared in Example 3 of the present invention was used twice a day for 7 consecutive days, and the patients' odor symptoms were alleviated or disappeared, with a total effective rate of 100%; after 14 consecutive days, the cure rate was 86%, and no recurrence was found during a 2-month follow-up.

[0049] For matters not mentioned above, the prior art applies.

[0050] Although some specific embodiments of the present invention have been described in detail through examples, those skilled in the art should understand that the above examples are for illustration only and are not intended to limit the scope of the present invention. Those skilled in the art to which the present invention belongs may make various modifications or supplements to the specific embodiments described or replace them in a similar manner, but they will not deviate from the direction of the present invention or exceed the scope defined by the attached claims. Those skilled in the art should understand that any modifications, equivalent substitutions, improvements, etc. made to the above embodiments based on the technical essence of the present invention should be included in the protection scope of the present invention.

Claims

1. An antibacterial composition, characterized in that Includes Sophora flavescens alkaloids and plant enzymes.

2. An antibacterial composition according to claim 1, characterized in that: The mass volume ratio of the sophora flavescens alkaloids and the plant enzyme is 1-3:10-30 g / ml.

3. An antibacterial composition according to claim 1, characterized in that: The matrine alkaloids include one or more of matrine, oxymatrine, hydroxymatrine, n-methyl cytisine, anagerine, bapeline and dehydromatrine.

4. An antibacterial composition according to claim 1, characterized in that: The matrine alkaloids include matrine and oxymatrine.

5. An antibacterial composition according to claim 1, characterized in that: The sophora flavescens alkaloid is prepared by the following method: the sophora flavescens medicinal material is crushed and passed through a 40-mesh sieve, the extraction solvent is 60% ethanol, the solid-liquid ratio is 1:8-12, reflux extraction is performed for 3 times, each time for 30 minutes, the extraction temperature is 40°C, the extract is centrifuged at 4500r / min for 15 minutes to obtain a supernatant; the supernatant is decompressed and filtered, the filtrate is taken, and rotary evaporation is performed until there is no ethanol taste, an appropriate amount of concentrated ammonia test solution is added to alkalize to pH=9-10, and chloroform is used for multiple extractions at 1:1 until there is no color, the chloroform layers are mixed, the solvent is recovered to a dry paste, and an HPD-100 macroporous resin is used for adsorption and purification, and the purified analytical solution is decompressed and concentrated to remove ethanol, and then freeze-dried to obtain the obtained solution.

6. An antibacterial composition according to claim 1, characterized in that: Bacteria include Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans.

7. Use of the antibacterial composition according to any one of claims 1 to 6 in the preparation of an external preparation for preventing and treating body odor.

8. The use according to claim 7, characterized in that The external preparation is a spray, a lotion, a tincture, an ointment, or a gel.

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