Reagent reaction device and gene sequencing system

Abstract

The invention relates to the technical field of gene sequencing, in particular to a heating structure of a reagent liquid supply pipe. The heating wire is wound on the reagent liquid supply pipe of the reagent reaction device, and the reagent in the reagent liquid supply pipe can be heated through the heating wire, so that the temperature of the reagent entering the reaction container is increased, and the difference value between the temperature of the reagent entering the reaction container and the temperature in the reaction container is reduced. The heating power of the heating wire is controlled by the controller, so that the temperature of the reagent in the reagent supply pipe can be kept at a reasonable level. Compared with the existing mode of adding the reagent into the reaction container for heating, the temperature of the reagent added into the reaction container is increased by heating in the reagent liquid supply pipe; the technical problem that in an existing sequencing system, the detection result accuracy is poor due to the fact that the temperature difference between the temperature of a reagent entering a reaction container and the set temperature of the reaction container is large is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of gene sequencing, and in particular to a heating structure of a reagent liquid supply tube. Background Art

[0002] In many biological or chemical reaction processes, the efficiency of reagent reaction has a great influence on the final reaction results. The efficiency of reagent reaction in some links is very sensitive to temperature. For example, many enzymes in mammals have the best activity only at or near the body temperature of mammals (i.e., 36°C to 40°C); for another example, many enzymes involved in biochemical reactions (such as polymerase) come from thermophilic bacteria. In the reactions involving such enzymes, if you want to obtain higher reaction efficiency, you need to carry out the reactions at high temperature (50°C-80°C).

[0003] During the use of the reagents containing the above enzymes, if they are stored at room temperature for a long time, it is easy to cause the reagents to fail and the performance to be unstable. Therefore, the above enzyme-containing reagents are usually stored in a low-temperature environment. However, since the optimal reaction environment temperature of the reagents containing enzymes is relatively high, after the reagents are taken out of the low-temperature environment, it takes a certain amount of time to raise the temperature of the reagents. At present, this heating process is usually carried out in a reaction vessel, which reduces the reaction efficiency of the reagents to a certain extent. For example, in the gene sequencing process, most of the reagents need to be stored in a low-temperature environment (2°C-8°C), and the low-temperature reagents are pumped into a high-temperature reaction vessel for a high-temperature environment reaction. In order to maintain the uniformity of the reaction efficiency in the reaction vessel, the temperature of the reagents in the entire reaction vessel is required to be consistent, and the temperature difference is not greater than 0.5°C. Since many sequencing processes are carried out in cycles, each cycle includes different detection steps, and the detection reagents involved in each detection step may be different, and the reagents need to be pumped from the low-temperature environment into the high-temperature reaction vessel. At present, a heating stage is commonly used to heat the reaction container to provide a high-temperature reaction environment for the reagents in the reaction container. This reagent heating method has the following problems: 1) After the low-temperature reagent enters the reaction container, the reagent temperature is quite different from the set temperature of the reaction container, and the reagent heating time is long. During the time when the reagent is heated, some side reactions or incomplete reactions are prone to occur, resulting in poor detection accuracy. 2) The temperature of the area near the reagent inlet in the reaction container is low, and the temperature of the area near the reagent outlet is high, resulting in a large temperature difference between different areas of the reaction container, and there are many inconsistent detection results in different areas. In summary, the current sequencing system has a large difference between the temperature of the reagent entering the reaction container and the set temperature of the reaction container, resulting in a technical problem of poor detection accuracy. Summary of the invention

[0004] An object of the present invention is to provide a reagent reaction device for improving the technical problem of poor detection result accuracy caused by the large difference between the reagent temperature entering the reaction container and the set temperature of the reaction container in the current sequencing system; in addition, an object of the present invention is also to provide a gene sequencing system using the above-mentioned reagent reaction device.

[0005] According to the first aspect, an embodiment provides a reagent reaction device, comprising a reagent liquid supply tube, a heating wire and a reaction container; the reagent liquid supply tube has a liquid inlet end and a liquid outlet end; the liquid outlet end is connected to the reaction container so as to supply liquid to the reaction container; the heating wire is wound around the reagent liquid supply tube to heat the reagent in the reagent liquid supply tube;

[0006] The reagent reaction device comprises a controller, and the controller is capable of adjusting the heating power of the heating wire.

[0007] Furthermore, in one embodiment, the heating wire is wound around the reagent supply tube to form a spiral coil, and the heating wire has at least two wiring terminals for wiring, and each wiring terminal is located at the same end of the spiral coil.

[0008] Furthermore, in one embodiment, the spiral coil is a double-strand spiral coil, the heating wire includes a first strand of wire and a second strand of wire, the first strand of wire and the second strand of wire are wound in parallel on the reagent supply tube, one end of the first strand of wire is connected to one end of the second strand of wire so that the first strand of wire and the second strand of wire are connected in series, and the other end of the first strand of wire and the other end of the second strand of wire are both the wiring terminals.

[0009] Furthermore, in one embodiment, the heating wire is wound around the reagent liquid supply tube to form a spiral coil, and the spiral coil is close to the liquid outlet and away from the liquid inlet.

[0010] Furthermore, in one embodiment, the reagent reaction device includes a temperature sensor, which is used to detect the temperature of the reagent supply tube or the reaction container. The temperature sensor is communicatively connected to the controller, and the controller adjusts the heating power of the heating wire according to the data of the temperature sensor.

[0011] Furthermore, in one embodiment, at least one of the temperature sensors is far away from the liquid inlet end and close to the liquid outlet end.

[0012] Furthermore, in one embodiment, at least two temperature sensors are arranged in the extending direction of the reagent supply tube.

[0013] Furthermore, in one embodiment, the reagent reaction device includes a temperature sensor, and the temperature sensor is used to detect the temperature of the reaction container. The temperature sensor is communicatively connected to the controller, and the controller adjusts the heating power of the heating wire according to data from the temperature sensor.

[0014] Furthermore, in one embodiment, the heating wire is wound around the reagent supply tube to form a spiral coil, and the pitch of the spiral coil is greater than the diameter of the heating wire.

[0015] Furthermore, in one embodiment, the reagent liquid supply tube is an insulating tube, and a receiving groove for receiving a single-turn coil in the spiral coil is provided on the reagent liquid supply tube, and two adjacent turns of the coil are separated by a groove wall of the receiving groove.

[0016] Furthermore, in one embodiment, the reagent liquid supply tube is an insulating tube, and an insulating protrusion is provided on the reagent liquid supply tube, and the insulating protrusion is used to separate two adjacent turns of the coil in the heating wire.

[0017] Furthermore, in one embodiment, the reagent liquid supply tube is provided with a tube insulation layer on its periphery, and the heating wire is located between the tube insulation layer and the reagent liquid supply tube.

[0018] Furthermore, in one embodiment, the outer periphery of the heating wire is coated with an insulating layer.

[0019] According to the second aspect, an embodiment provides a gene sequencing system, including a reagent reaction device, wherein the reagent reaction device is the reagent reaction device described in any one of the above embodiments, and the gene sequencing system also includes a container heating device for heating the reaction container to maintain the temperature inside the reaction container.

[0020] According to the reagent reaction device of the above embodiment, a heating wire is wound around the reagent supply tube of the reagent reaction device, and the reagent in the reagent supply tube can be heated by the heating wire, so that the temperature of the reagent entering the reaction vessel is improved, and the difference between the temperature of the reagent entering the reaction vessel and the temperature in the reaction vessel is reduced. The heating power of the heating wire can be adjusted by the controller to keep the temperature of the reagent in the reagent supply tube at a reasonable level. Compared with the current method of heating up the reagent added to the reaction vessel, the present application improves the temperature of the reagent added to the reaction vessel by preheating the reagent in the reagent supply tube, and improves the technical problem of poor accuracy of the detection result caused by the large difference between the temperature of the reagent entering the reaction vessel and the set temperature of the reaction vessel in the current sequencing system. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A liquid circuit diagram of a gene sequencing system in an embodiment;

[0022] Figure 2 for Figure 1 A magnified view of part A;

[0023] Figure 3 It is a schematic diagram of a power supply circuit where the heating wire is located in an embodiment.

[0024] List of feature names corresponding to the figure marks in the figure: 1. Reagent supply tube; 11. Liquid inlet end; 12. Liquid outlet end; 2. Heating wire; 21. Wiring terminal; 22. First wire strand; 23. Second wire strand; 3. Reaction container; 4. Power supply circuit; 5. Controller; 6. Reversing valve; 7. Reagent container; 8. Temperature sensor; 9. Suction pump; 10. Waste container. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are for making the present application better understood. However, those skilled in the art can easily recognize that some features can be omitted in different situations, or can be replaced by other elements, materials, methods. In some cases, some operations related to the present application are not shown or described in the specification, this is to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations according to the description in the specification and the general technical knowledge in the art.

[0026] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be interchanged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a required sequence, unless otherwise specified that a certain sequence must be followed.

[0027] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0028] In view of the problem that the temperature of the reagent in the reaction container of the current sequencing system is greatly different from the set temperature, the present application adopts preheating before the reagent is added to the reaction container, by winding the heating wire on the reagent supply tube, the reagent is preheated, so that the reagent entering the reaction container has a certain temperature, and the temperature difference with the temperature set in the reaction container is reduced, and the temperature difference between different areas in the reaction container is reduced, and the reagent is heated by the container heating device that heats the reaction container, so that the reagent reaches the set temperature. In this way, the reagent added to the reaction container can quickly reach the set temperature, and the possibility of the reagent having a side reaction or incomplete reaction during the temperature rise process is reduced, which improves the accuracy of the sequencing system detection result. In addition, the method of winding the heating wire on the reagent supply tube does not affect the deformation movement of the reagent supply tube, does not change the original structure of the sequencing system, and can be applied to the modification of the old sequencing system, and can also be applied to the production of the new sequencing system. The reagent reaction device and gene sequencing system of the present application are introduced in detail below in conjunction with the accompanying drawings.

[0029] Please refer to Figures 1 to 3 In some embodiments, the reagent reaction device includes a reagent liquid supply tube 1, a heating wire 2, a reaction container 3, and a power supply circuit 4 (please refer to Figure 3 ) and controller 5.

[0030] The reagent liquid supply tube 1 has a liquid inlet end 11 and a liquid outlet end 12, wherein the liquid outlet end 12 is connected to the reaction container 3 so as to be able to supply liquid to the reaction container 3. The liquid inlet end 11 is used to connect a liquid supply device of the reagent. In one embodiment, the liquid supply device includes a reversing valve 6 and a reagent holding container 7, wherein the reagent holding container 7 contains a plurality of different reagents, and the reversing valve 6 is used to switch the reagent liquid supply tube 1 to be connected to different reagents, thereby supplying different types of reagents to the reaction container 3.

[0031] The connection described in the present application includes both direct connection and indirect connection through other components. For example, the connection between the liquid inlet end 11 and the liquid supply device includes both the liquid inlet end 11 being directly connected to the reversing valve 6 of the liquid supply device and other devices being arranged between the liquid inlet end 11 and the reversing valve 6, such as a filter.

[0032] The heating wire 2 is wound around the reagent supply tube 1 and is used to heat the reagent in the reagent supply tube 1. The power supply circuit 4 supplies power to the heating wire 2, and the controller 5 is connected to the power supply circuit 4 to adjust the heating power of the heating wire 2. Specifically, the controller 5 can adjust the heating power of the heating wire 2 by adjusting the power supply voltage of the heating wire 2, and can also adjust the heating power of the heating wire 2 by adjusting the current of the heating wire 2.

[0033] In the present application, the reagent liquid supply tube 1 is heated by winding a heating wire 2 on the reagent liquid supply tube 1, and the heating power of the heating wire 2 is adjusted by the controller 5 to increase the temperature of the reagent entering the reaction container 3, thereby reducing the temperature difference between the reagent temperature and the set temperature in the reaction container 3, and also reducing the temperature difference between different areas in the reaction container 3, thereby improving the technical problem of low accuracy of the detection result caused by the large temperature difference of the reagent in the reaction container 3 of the current sequencing system.

[0034] In one embodiment, after the heating wire 2 is wound around the reagent supply tube 1, it can be fixed by glue bonding, binding or tape bonding.

[0035] The heating wire 2 can be wound in the reagent supply tube 1 in any feasible manner:

[0036] For example, in one embodiment, the outer periphery of the heating wire 2 is coated with an insulating layer, and the insulating layer can prevent short circuit between two adjacent single-turn coils after the heating wire 2 is wound. The insulating layer is made of insulating non-metallic material, specifically insulating rubber, insulating resin, etc. For example, the heating wire 2 and the insulating layer can form an enameled wire in the prior art.

[0037] For another example, in one embodiment, the heating wire 2 is wound on the reagent supply tube 1 to form a spiral coil. In order to prevent the conductive contact between two adjacent single-turn coils after the heating wire 2 is wound and cause a short circuit, a receiving groove is provided on the reagent supply tube 1. The single-turn coil of the heating wire 2 is in the receiving groove. The two adjacent single-turn coils are separated by the groove wall of the receiving groove. In this case, the reagent supply tube 1 should be an insulating tube. Specifically, the receiving groove can be a spiral groove.

[0038] For another example, in one embodiment, a protrusion may be provided on the reagent liquid supply tube 1, and the protrusion may be used to separate two adjacent single-turn coils of the heating wire 2 to prevent the two adjacent single-turn coils of the heating wire 2 from contacting and short-circuiting. Furthermore, in one embodiment, when a protrusion or a receiving groove is used to separate adjacent single-turn coils of the heating wire 2, a tube insulation layer is provided on the periphery of the liquid supply tube, and the heating wire 2 is located between the tube insulation layer and the liquid supply tube. The tube insulation layer may prevent the heating wire 2 from being accidentally touched from the outside, and the safety is better.

[0039] It should be noted that in the above embodiment, the single-turn coil described is based on the heating wire 2 being spirally wound around the reagent supply tube 1 to form a spiral coil. It should be understood that the pitch of the spiral coil is the axial distance between the center points of two adjacent turns of the coil on the mid-diameter, that is, the axial distance between the center lines of the cross-sections of two adjacent effective turns.

[0040] It should be understood that the ends of two adjacent turns of the spiral coil are actually connected together, and the two adjacent turns of the coil described in this application are separated, which does not mean that the two turns of the coil are completely separated, but that the other parts are separated and do not touch except the position where the two ends are connected. Alternatively, it can also be understood that the two adjacent turns of the coil are separated in order to realize that the two adjacent turns of the coil are connected in series to avoid short circuit caused by contact between other parts of the two.

[0041] In some other embodiments, in addition to the spiral winding method, the heating wire 2 can also be wound in any other feasible way. For example, the heating wire 2 can be formed into a heating net by reciprocating bending, and then the heating net is wrapped around the periphery of the reagent supply tube 1 and fixed to the reagent supply tube 1. Specific fixing methods may include bonding fixation, binding fixation, etc.

[0042] In one embodiment, please refer to Figure 1 and Figure 2 The heating wire 2 is wound around the reagent liquid supply tube 1 to form a spiral coil, and the pitch of the spiral coil is greater than the diameter of the heating wire 2. In this way, there is a gap between two adjacent turns of the coil. In addition to maintaining the gap between the two adjacent turns of the coil by the insulating material mentioned above, the heating wire 2 and the reagent liquid supply tube 1 can also be fixed to keep the pitch of the spiral coil unchanged. The specific fixing method includes bonding, tying or pouring an insulating layer covering the heating wire 2.

[0043] The reagent liquid supply tube 1 in the present application can be made of non-metallic insulating materials or a metal tube. When a metal tube is used and insulation is required, an insulating sleeve can be put on the outer sleeve of the metal tube to meet the installation requirements of the heating wire 2.

[0044] For some examples, please refer to Figure 2 The heating wire 2 is wound around the reagent supply tube 1 to form a spiral coil. The heating wire 2 has at least two wiring terminals 21 for wiring, and each wiring terminal 21 is at the same end of the spiral coil. By making the wiring terminals 21 at the same end of the spiral coil, it is convenient to connect the heating wire 2 to the power supply circuit 4, and simplify the layout of the circuit. Specifically, in one embodiment, please refer to Figure 2 The spiral coil is wound by the same heating wire 2, and the heating wire 2 has two terminals 21, and the two terminals 21 are at the same end of the spiral coil. In some other embodiments, the terminals 21 of the heating wire 2 can also be partially at one end of the spiral coil and the other part at the other end of the spiral coil.

[0045] For some examples, please refer to Figure 2 and Figure 3 , the power supply circuit 4 is connected to the controller 5 , and the terminal 21 is also connected to the controller 5 .

[0046] Further, in one embodiment, please refer to Figure 2 , the spiral coil is a double-strand spiral coil, the heating wire 2 includes a first strand 22 and a second strand 23, the first strand 22 and the second strand 23 are wound in parallel on the reagent supply tube 1, one end of the first strand 22 is connected to one end of the second strand 23, so that the first strand 22 and the second strand 23 are connected in series, and the other end of the first strand 22 and the other end of the second strand 23 are both terminal 21. Specifically, in one embodiment, the first strand 22 and the second strand 23 are integrally formed, at this time there is only one heating wire 2, and the same heating wire 2 is divided into two strands, which is more efficient when the heating wire 2 is wound, thereby improving the manufacturing efficiency.

[0047] Of course, in some other embodiments, the first wire strand 22 and the second wire strand 23 may also be two separate wire strands. In this case, according to actual needs, one end of the first wire strand 22 and one end of the second wire strand 23 are connected together before or after winding. The specific connection method may include welding, knotting and overlapping, or connecting through an electrical connector.

[0048] In some other embodiments, the spiral coil in the present application may also be a single-strand spiral coil, or a three-strand spiral coil or other feasible types. In some other embodiments, when the spiral tube of the heating wire 2 is a double-strand spiral coil, the first strand 22 and the second strand 23 may also be independent of each other, and the first strand 22 and the second strand 23 are independently connected to the power supply circuit 4, and the two may be connected in series or in parallel.

[0049] In one embodiment, please refer to Figure 1 and Figure 2 In order to reduce heat loss, the heating wire 2 is wound around the reagent supply tube 1 to form a spiral coil, which is close to the liquid outlet 12 and away from the liquid inlet 11. The spiral coil close to the liquid outlet 12 can make the heated reagent enter the reaction container 3 as soon as possible, reducing heat loss.

[0050] Regarding the way in which the controller 5 controls the heating wire 2, any feasible solution may be adopted:

[0051] For example, in some embodiments, please refer to Figure 2 The reagent reaction device includes a temperature sensor 8, which is used to detect the temperature of the reagent supply tube 1. The temperature sensor 8 is connected to the controller 5 for communication. The controller 5 adjusts the heating power of the heating wire 2 according to the data of the temperature sensor 8. The temperature of the reagent supply tube 1 is obtained by the temperature sensor 8, and the temperature of the reagent in the reagent supply tube 1 can be adjusted in time.

[0052] For another example, in some embodiments, the reagent reaction device includes a temperature sensor 8, which is used to detect the temperature of the reaction container 3. The temperature sensor 8 is communicatively connected to the controller 5, and the controller 5 adjusts the heating power of the heating wire 2 according to the data of the temperature sensor 8.

[0053] For another example, in some embodiments, the controller 5 can control the heating power of the heating wire 2 according to a preset automatic heating program. Of course, the preset automatic heating program needs to be verified in advance to obtain a suitable heating time and heating power.

[0054] When the temperature sensor 8 for measuring the reagent supply tube 1 is used, the dynamic adjustment process of the controller 5 is: according to the detection value of the temperature sensor 8, the temperature of the reagent supply tube 1 is controlled, and when the temperature of the reagent supply tube 1 is lower than the set value, the controller 5 adjusts the heating power of the heating wire 2 or turns on the heating. That is to say, in the present application, the controller 5 adjusts the heating power of the heating wire 2, including increasing or reducing the heating power of the heating wire 2, and also includes closing or opening the working state of the heating wire 2, wherein closing the heating wire 2 is equivalent to reducing the power of the heating wire 2 to 0, and opening the heating wire 2 is equivalent to increasing the power of the heating wire 2 to the starting state of the heating wire 2. Specifically, in one embodiment, when the temperature of the temperature sensor 8 is higher than the set target temperature, the controller 5 controls the heating wire 2 to reduce the heating power until the temperature of the temperature sensor 8 reaches the set target temperature; when the temperature of the temperature sensor 8 is lower than the set target temperature, the controller 5 controls the heating wire 2 to increase the heating power until the temperature of the temperature sensor 8 reaches the set target temperature. During the flow of the reagent, the temperature of the reagent supply tube 1 is in a dynamically changing state, and through dynamic adjustment, the ultimate goal is to dynamically maintain the temperature of the reagent supply tube 1 at the set target value.

[0055] Further, in some embodiments, please refer to Figure 2 , at least one temperature sensor 8 is arranged away from the liquid inlet end 11 and close to the liquid outlet end 12. The temperature measured by the temperature sensor 8 close to the liquid outlet end 12 is closer to the temperature of the reagent entering the reaction container 3, and the temperature adjustment of the reagent is more accurate. Of course, in some other embodiments, the temperature sensor 8 can be arranged close to the liquid inlet end 11 and away from the liquid outlet end 12. In some other embodiments, the temperature sensor 8 can also be arranged in the middle of the length direction of the spiral coil.

[0056] Specifically, in one embodiment, please refer to Figure 2 , only one temperature sensor 8 is provided. The temperature sensor 8 can be a thermistor.

[0057] In some other embodiments, at least two temperature sensors 8 are arranged in the extending direction of the reagent supply tube 1. At least two temperature sensors 8 can more accurately grasp the change of the reagent temperature in the reagent supply tube 1, and facilitate the adjustment of the heating power of the heating wire 2. The specific number of temperature sensors 8 can be set as needed, such as two, three or more than four.

[0058] In one embodiment, in order to facilitate the winding of the heating wire 2, the heating wire 2 is made of a nickel-based alloy. The resistance wire of the nickel-based alloy has good ductility, which is conducive to the deformation of the heating wire 2. In some other embodiments, the heating wire 2 can also be made of any other feasible metal alloy material.

[0059] After research, the applicant found that the wire diameter of the heating wire 2 and the tube diameter of the reagent supply tube 1 will affect the tightness of the heating wire 2 wrapped around the pipeline. When the wire diameter of the heating wire 2 is too thick, the heating wire 2 is loosely wrapped around the reagent supply tube 1, and the spiral coil is easy to loosen. When the reagent supply tube 1 needs to be moved during the operation of the sequencing system, it is easy to cause the position of the heating wire 2 to move. When the wire diameter of the heating wire 2 is too thin, it is tighter when wrapped around the reagent supply tube 1, but it is also easy to tighten and leave marks on the pipeline. Too deep tightening will affect the pipeline. In some embodiments, the ratio of the outer diameter of the reagent supply tube 1 to the wire diameter of the heating wire 2 is 5 to 10, which can prevent the heating wire 2 from being easily loosened after being wound on the reagent supply tube 1, and also prevent the heating wire 2 from being too thin and causing marks on the reagent supply tube 1.

[0060] Specifically, in one embodiment, the tube diameter is 1 / 16" OD, and the heating wire 2 has a wire diameter of 0.2 mm. The heating wire 2 and the outer insulating layer form an enameled wire, and the enameled wire is wound to form a spiral coil with a spiral pitch of less than 1 mm. The length of the spiral coil can be set as needed, for example, the length can be more than 5 cm.

[0061] In some other embodiments, the ratio of the outer diameter of the reagent supply tube 1 to the wire diameter of the heating wire 2 may be less than 5 or greater than 10 as required.

[0062] For some examples of gene sequencing systems, please refer to Figure 1 and Figure 2 The gene sequencing system includes a reagent reaction device, which is the reagent reaction device described in any of the above embodiments and will not be described in detail. The gene sequencing system also includes a container heating device for heating the reaction container 3 to maintain the temperature in the reaction container 3. The gene sequencing system also includes a liquid supply device, a suction pump 9 and a waste container 10, and the liquid supply device includes a reagent container 7 and a reversing valve 6.

[0063] When the reagent of the gene sequencing system flows in the path, the suction pump works to draw the reagent in the reagent container 7 into the reaction container 3 for reaction. After the reagent is drawn out, it first passes through the reversing valve 6, and the type of the extracted reagent can be changed through the reversing valve 6. Then, it passes through the reagent supply pipe 1 and enters the reaction container 3. After entering the reaction container 3 for reaction, the reagent enters the waste container 10 through the suction pump 9.

[0064] The above specific examples are used to illustrate the present invention, which is only used to help understand the present invention and is not intended to limit the present invention. For those skilled in the art, according to the concept of the present invention, some simple deductions, modifications or substitutions can be made.

Claims

1. A reagent reaction device, characterized in that: The invention comprises a reagent liquid supply tube, a heating wire and a reaction container; the reagent liquid supply tube has a liquid inlet end and a liquid outlet end; the liquid outlet end is connected to the reaction container so as to supply liquid to the reaction container; the heating wire is wound around the reagent liquid supply tube so as to heat the reagent in the reagent liquid supply tube; The reagent reaction device comprises a controller and a power supply circuit, the power supply circuit supplies power to the heating wire, and the controller is connected to the power supply circuit so as to be able to adjust the heating power of the heating wire.

2. The reagent reaction device according to claim 1, characterized in that: The heating wire is wound around the reagent liquid supply tube to form a spiral coil. The heating wire has at least two wiring terminals for wiring, and each wiring terminal is located at the same end of the spiral coil.

3. The reagent reaction device according to claim 2, characterized in that: The spiral coil is a double-strand spiral coil, and the heating wire includes a first wire and a second wire. The first wire and the second wire are wound in parallel on the reagent supply tube, one end of the first wire is connected to one end of the second wire so that the first wire and the second wire are connected in series, and the other end of the first wire and the other end of the second wire are both the wiring terminals.

4. The reagent reaction device according to claim 1, characterized in that: The heating wire is wound around the reagent liquid supply tube to form a spiral coil, and the spiral coil is close to the liquid outlet and far away from the liquid inlet.

5. The reagent reaction device according to any one of claims 1 to 4, characterized in that: The reagent reaction device includes a temperature sensor, which is used to detect the temperature of the reagent liquid supply tube. The temperature sensor is communicatively connected with the controller, and the controller adjusts the heating power of the heating wire according to data from the temperature sensor.

6. The reagent reaction device according to claim 5, characterized in that: At least one of the temperature sensors is located away from the liquid inlet and close to the liquid outlet.

7. The reagent reaction device according to claim 5, characterized in that: At least two temperature sensors are arranged in the extending direction of the reagent supply tube.

8. The reagent reaction device according to any one of claims 1 to 4, characterized in that: The reagent reaction device includes a temperature sensor, which is used to detect the temperature of the reaction container. The temperature sensor is communicatively connected with the controller, and the controller adjusts the heating power of the heating wire according to data from the temperature sensor.

9. The reagent reaction device according to claim 1, characterized in that: The heating wire is wound around the reagent liquid supply tube to form a spiral coil, and the pitch of the spiral coil is greater than the diameter of the heating wire.

10. The reagent reaction device according to claim 9, characterized in that: The reagent liquid supply tube is an insulating tube, and a receiving groove for receiving a single-turn coil in the spiral coil is provided on the reagent liquid supply tube, and two adjacent turns of the spiral coil are separated by a groove wall of the receiving groove.

11. The reagent reaction device according to claim 9, characterized in that: The reagent liquid supply tube is an insulating tube, and an insulating protrusion is arranged on the reagent liquid supply tube. The insulating protrusion is used to separate two adjacent turns of the spiral coil.

12. The reagent reaction device according to claim 10 or 11, characterized in that: The reagent liquid supply tube is provided with a tube insulation layer on its periphery, and the heating wire is located between the tube insulation layer and the reagent liquid supply tube.

13. The reagent reaction device according to any one of claims 1 to 4, characterized in that: The outer periphery of the heating wire is covered with an insulating layer.

14. A gene sequencing system, comprising a reagent reaction device, wherein the reagent reaction device is the reagent reaction device according to any one of claims 1 to 13, and the gene sequencing system also comprises a container heating device for heating the reaction container to maintain the temperature inside the reaction container.

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