Efficient sampling device for detecting pathogenic bacteria in food and sampling detection method

Abstract

The invention provides an efficient sampling device for detecting pathogenic bacteria in food and a sampling detection method.The efficient sampling device comprises a bottom plate and a storage device, the upper surface of the bottom plate is fixedly connected with a placement plate through the storage device, and a sampling mechanism is arranged on the side, close to the placement plate, of the bottom plate; the sampling mechanism comprises two bases, the two bases are fixedly connected with the bottom plate, sliding blocks are slidably connected to the inner walls of the bases, three insertion holes are formed in the side face of one base, and a plug pin slidably penetrates through the inner wall of one insertion hole. According to the food sampling device, by arranging the sampling mechanism, when food is sampled, the food can be sampled through the sampling mechanism, the food sampling efficiency can be improved through the sampling mechanism, the accuracy of subsequent food detection is improved, multiple positions of the same kind of food can be sampled, and the sampling efficiency is improved. And the convenience of sampling other parts of the article is improved.

Description

Technical Field

[0001] The invention relates to the technical field of food pathogen sampling and gene detection, and in particular to a high-efficiency sampling device and a sampling detection method for detecting pathogenic bacteria in food. Background Art

[0002] Pathogenic bacteria are the main cause of foodborne diseases. Pathogenic microorganisms can grow in improperly handled or stored food. Sampling and testing of pathogenic bacteria in food is a core part of food safety management and can effectively prevent the occurrence of foodborne diseases.

[0003] Food sampling equipment is a special tool or machine used to collect samples from food batches to ensure the representativeness and accuracy of the samples for subsequent quality control, hygiene inspection, ingredient analysis and other testing.

[0004] In the prior art, the invention patent with publication number CN108426731A specifically discloses a food detection sampling device, and the technical solution thereof is as follows: it comprises a tube body, the outer wall of the tube body is provided with a rubber sleeve, the right side of the tube body is provided with a cannula, the front and rear sides of the cannula are provided with a first slide groove, the upper and lower sides of the cannula are provided with a second slide groove, the outer wall of the cannula is sleeved with a retaining ring, the retaining ring is overlapped with the cannula, the inner front and rear ends of the retaining ring are provided with a first slider, and the first slider is overlapped with the first slide groove. The food detection sampling device, when sampling food detection, drives the cannula through the tube body, inserts the cannula into the inner side of the food, contacts the retaining ring through the food, drives the pull rod to move through the second slider, drives the needle flap together through the pull rod, wraps the food inside the video inside the needle flap, and completes the sampling by pulling out the cannula, so that the operation is simple, sampling of different depths of food can be achieved, and a favorable sample is brought for food detection.

[0005] Regarding the above-mentioned relevant contents, the following technical defects exist: sampling is usually performed by cutting food with a cutter to achieve the purpose of sampling, but the original sampling method has a slow sampling efficiency, and there are certain safety hazards when using a cutter, and the quality of each sampling is different, which also leads to a decrease in the accuracy of subsequent sampling results. In addition, there are many types and forms of food, such as liquid, solid or viscous paste, etc. If multiple sampling mechanisms need to be prepared for different forms, not only will sampling be inconvenient, but it will also increase the cost of additional equipment. And safety issues also need to be considered, especially when sampling some spoiled and rotten foods to detect pathogens, special attention should be paid to minimizing leakage and other problems during the sampling process to avoid human contact and disease.

[0006] Therefore, it is necessary to provide a new and efficient sampling device for detecting pathogenic bacteria in food to solve the above technical problems. Summary of the invention

[0007] The purpose of the present invention is to solve the shortcomings of the prior art and to propose an efficient sampling device for detecting pathogenic bacteria in food.

[0008] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: it includes a bottom plate and a storage device, the upper surface of the bottom plate is fixedly connected to a placement plate by means of the storage device, a sampling mechanism is provided on the side of the bottom plate close to the placement plate, the sampling mechanism includes two bases, both of the bases are fixedly connected to the bottom plate, the inner wall of the base is slidably connected to a sliding block, one of the bases is provided with three plug holes on the side surface, a latch is slidably penetrated through the inner wall of one of the plug holes, the latch is slidably penetrated through the inner wall of the sliding block, the arc surface of the latch is slidably connected to a positioning ring, the arc surface of the latch is sleeved with a first spring, the two ends of the first spring are respectively connected to The latch pin is fixedly connected to the positioning ring, and the two sliding blocks are fixedly connected to a support column on one side away from the bottom plate, and the positioning ring is fixedly connected to one of the support columns, and one side of one of the support columns is fixedly connected to a motor, and the output end of the motor is fixedly connected to an adjusting rod, and the arc surface of the adjusting rod rotates through the inner wall of the other support column, and the end of the adjusting rod away from the arc surface of the motor is fixedly connected to a rotating block, and three slots are provided on the side of the rotating block away from the support column, and the inner wall of one of the slots is slidably connected to a limiting block, and a first electric push rod is provided on one side of the limiting block, and the first electric push rod is fixedly connected to the other support column. The output end of the first electric push rod is fixedly connected to the limit block, the arc surface of the adjusting rod is fixedly connected to a fixing plate, one side of the fixing plate is fixedly connected to the second electric push rod, the output end of the second electric push rod is fixedly connected to a connecting block, the connecting block is fixedly connected to a sampling sleeve at an output end away from the second electric push rod, the top wall of the sampling sleeve is fixedly connected to a second spring, one end of the second spring away from the fixing plate is fixedly connected to a pressing rod, the pressing rod slides through the inner wall of the sampling sleeve, a sliding groove is provided on one side of the placing plate, the inner wall of the sliding groove is slidably connected to a sliding rod, one end of the arc surface of the sliding rod is fixedly connected to an adjusting clamp, the placing A fixed clamp is fixedly connected to one side of the plate close to the adjusting clamp, and two connecting rings are provided at one end of the side surface of the adjusting clamp, one of the connecting rings is fixedly connected to the adjusting clamp, and the other connecting ring is fixedly connected to the fixed clamp, and two fixing rings are provided at the other end of the side surface of the adjusting clamp, one of the fixing rings is fixedly connected to the adjusting clamp, and the other fixing ring is fixedly connected to the fixed clamp, the inner walls of the two connecting rings are provided with the same first rotating rod, the first rotating rod is threadedly connected to one of the connecting rings, the first rotating rod is rotatably connected to the other connecting ring, and the inner walls of the two fixing rings are slidably penetrated by the same guide rod.

[0009] By adopting the above technical scheme, pathogenic bacteria is the main cause of foodborne diseases. Pathogenic microorganisms can breed in improperly handled or stored food. Sampling and detection of pathogenic bacteria in food is the core part of food safety management, which can effectively prevent the occurrence of foodborne diseases. Usually, sampling is performed by cutting the food with a cutter to achieve the sampling purpose. However, the sampling efficiency of the original sampling method is slow, and there are certain safety hazards when using a cutter. Moreover, the quality of each sampling is different, which also leads to a decrease in the accuracy of subsequent sampling results. At this time, the food can be sampled by a sampling mechanism. The sampling mechanism can improve the efficiency of food sampling and reduce the cost of manual sampling. At the same time, the sampling scale can also be controlled, thereby improving the accuracy of subsequent sample detection. The sampling mechanism can also be flipped, and different positions of the same sampled food can be sampled by flipping, further improving the convenience of multiple sampling of its food.

[0010] Preferably, a rotating disk is fixedly connected to one end of the arc surface of the first rotating rod, and the cross section of the rotating disk is circular.

[0011] By adopting this preferred solution, when the first rotating rod needs to be rotated, the first rotating rod can be rotated by the rotating disk installed on the first rotating rod, and the efficiency of rotating the first rotating rod can be improved by the rotating disk.

[0012] Preferably, a pull ring is rotatably connected to the inner wall of the latch.

[0013] By adopting the above technical solution, when the latch needs to be pulled, the latch can be pulled through the pull ring installed on the latch, and the speed and efficiency of pulling the latch can be improved by the pull ring.

[0014] Preferably, the sliding block is a titanium alloy block.

[0015] By adopting the above technical solution, the sliding block made of titanium alloy has a relatively hard surface when in use, is less likely to be deformed after long-term use, and has a longer service life.

[0016] Preferably, the cross section of the limiting block is arc-shaped.

[0017] By adopting the above technical solution, when the first electric push rod drives the limit block to contact the slot, the arc-shaped limit block has better contact stability with the slot, and the arc-shaped limit block can improve the contact effect when contacting the slot.

[0018] Preferably, a storage device is provided on a side of the bottom plate close to the placement plate, and the storage device includes a fixing frame, the fixing frame is fixedly connected to the bottom plate, a storage groove is opened on one side of the fixing frame, a storage box is slidably connected to the inner wall of the storage groove, three magnetic blocks are fixedly connected to one side of the storage box, a metal plate is adsorbed on a side of the three magnetic blocks away from the storage box, the metal plate is fixedly connected to a side of the inner wall of the storage groove, four fixing rods are fixedly connected to one end of the fixing frame away from the bottom plate, one end of the four fixing rods away from the arc surface of the fixing frame is fixedly connected to the placement plate, a plurality of guide holes are opened on the side of the placement plate, and the plurality of guide holes are evenly distributed on the placement plate.

[0019] By adopting the above technical solution, when sampling food through the sampling mechanism, the juice flowing out of the food sampling can be collected by the storage device, thereby preventing the juice from flowing everywhere and affecting the working environment of the entire sampling mechanism.

[0020] Preferably, a handle is fixedly connected to a side of the storage box away from the magnetic block.

[0021] By adopting the above technical solution, when the storage box needs to be pulled, the storage box can be moved by the handle installed on the storage box, thereby improving the efficiency of moving the storage box by the handle.

[0022] Preferably, a plurality of anti-slip grooves are provided on both sides of the handle, and the plurality of anti-slip grooves are evenly distributed on one side of the handle.

[0023] By adopting the above technical solution, when the storage box is moved by the handle, the anti-slip groove on the handle can increase the friction between the handle and the user's hand, thereby improving the stability when the handle is used.

[0024] Preferably, a contact device is provided on the side of the base plate away from the placement plate, and the contact device includes a contact plate, which is slidably connected to the base plate, and two assembly blocks are fixedly connected on both sides of the contact plate, and storage grooves are provided at positions of the contact plate corresponding to the four assembly blocks, and the inner wall thread of the assembly block is penetrated by a second rotating rod, and the second rotating rod is threadedly connected to the inner wall of the base plate.

[0025] By adopting the above technical solution, when the base plate contacts the desktop, the contact device can be installed on the base plate. The contact device can improve the stability of the base plate when in contact with the desktop, avoiding the small friction between the base plate and the desktop, which affects the stability of the base plate placed on the desktop.

[0026] Preferably, one end of the second rotating rod close to the arc surface of the bottom plate is fixedly connected with a guide block, and the guide block is funnel-shaped.

[0027] By adopting the above technical solution, when the second rotating rod is connected to the inner wall of the assembly block, the guide block installed on the second rotating rod can improve the connection efficiency between the second rotating rod and the assembly block, thereby fixing the contact plate to the base plate more quickly.

[0028] Compared with the prior art, the advantages and positive effects of the present invention are: 1. In the present invention, through the unique sampling mechanism, various forms of food including liquid, solid, and gel can be sampled at multiple locations, and the sampling process is completed by the device, which has multi-purpose characteristics, and the entire sampling process is safe and leak-free, which can effectively prevent the leakage, spread and infection of pathogenic bacteria. The sampling mechanism can improve the efficiency of food sampling, improve the accuracy of subsequent food testing, and can also sample multiple locations of the same food, improving the convenience of sampling other parts of the item.

[0029] 2. In the present invention, by providing a storage device, when the sampling mechanism samples the food, the juice overflowed from the food sampling can be collected by the storage device, thereby preventing the juice from affecting the environment of the entire sampling mechanism through collection.

[0030] 3. In the present invention, by providing a contact device, when the base plate is placed on the desktop, the contact device can be installed at the bottom of the base plate, so that the friction between the bottom of the base plate and the desktop can be increased through the contact device, thereby improving the overall sampling stability and reducing the possibility of the base plate sliding on the desktop. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 A schematic diagram of the three-dimensional structure of an efficient sampling device for detecting pathogenic bacteria in food proposed by the present invention; Figure 2 The present invention provides a schematic structural diagram of a sampling mechanism of an efficient sampling device for detecting pathogenic bacteria in food; Figure 3 for Figure 2 A magnified image of point A; Figure 4 A schematic diagram of the partially disassembled structure of a sampling mechanism of an efficient sampling device for detecting pathogenic bacteria in food proposed by the present invention; Figure 5 A partially enlarged structural schematic diagram of a sampling and grabbing mechanism of a high-efficiency sampling device for detecting pathogenic bacteria in food proposed by the present invention; Figure 6 The present invention provides a schematic structural diagram of a storage device for an efficient sampling device for detecting pathogenic bacteria in food; Figure 7A schematic diagram of the disassembly structure of a storage device of an efficient sampling device for detecting pathogenic bacteria in food proposed by the present invention; Figure 8 A schematic structural diagram of a contact device of an efficient sampling device for detecting pathogenic bacteria in food proposed by the present invention; Fig. 9 A schematic diagram of a partially disassembled structure of a contact device of an efficient sampling device for detecting pathogenic bacteria in food proposed by the present invention; Fig.10 for Figure 5 Schematic diagram of the decomposition structure.

[0032] Legend: 1. Bottom plate; 2. Sampling mechanism; 201. Base; 202. Sliding block; 203. Socket; 204. Latch; 205. First spring; 206. Positioning ring; 207. Support column; 208. Motor; 209. Adjustment rod; 210. Rotation block; 211. Slot; 212. First electric push rod; 213. Limit block; 214. Fixed plate; 215. Second electric push rod; 216. Connecting block; 217. Sampling sleeve; 218. Second spring; 219. Press rod; 220. Sliding slot; 221. Sliding rod ; 222, adjusting clamp; 223, connecting ring; 224, fixing ring; 225, guide rod; 226, fixing clamp; 227, first rotating rod; 228, turntable; 229, pull ring; 3, storage device; 31, fixing frame; 32, storage slot; 33, fixing rod; 34, guide hole; 35, storage box; 36, handle; 37, metal plate; 38, magnetic block; 39, anti-slip groove; 4, contact device; 41, contact plate; 42, assembly block; 43, storage slot; 44, guide block; 45, second rotating rod; 5, placement plate. DETAILED DESCRIPTION

[0033] In order to more clearly understand the above-mentioned purpose, features and advantages of the present invention, the present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0034] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0035] Embodiment 1, as Figure 1-9As shown, the present invention provides an efficient sampling device for detecting pathogenic bacteria in food, including a base plate 1 and a storage device 3, the upper surface of the base plate 1 is fixedly connected to a placement plate 5 by means of the storage device 3, a sampling mechanism 2 is provided on the side of the base plate 1 close to the placement plate 5, the storage device 3 is provided on the side of the base plate 1 close to the placement plate 5, and a contact device 4 is provided on the side of the base plate 1 away from the placement plate 5.

[0036] The specific configuration and functions of the sampling mechanism 2, the storage device 3 and the contact device 4 are described in detail below.

[0037] like Figure 2-Figure 5 As shown, the sampling mechanism 2 includes two bases 201 arranged on the bottom plate 1, and the two bases 201 are respectively located on the left and right sides of the placement plate 5. The inner wall of the base 201 is slidably connected with a sliding block 202, and the upper side of the sliding block 202 is fixedly connected with a support column 207, and one side of one of the support columns 207 is fixedly connected with a motor 208, and the output end of the motor 208 is fixedly connected with an adjustment rod 209, which passes through the two support columns 207 and is rotatably connected to the inner wall of the other support column 207. The sliding block 202 can move forward and backward along the base 201, thereby driving the support column 207 and the adjustment rod 209 to move forward and backward synchronously.

[0038] The sides of the two bases 201 are provided with three sockets 203 arranged side by side in a horizontal direction, and a latch 204 is slidably penetrated through the inner wall of one of the sockets 203, and the latch 204 is slidably penetrated through the inner wall of the sliding block 202, that is, one end of the latch 204 passes through the sliding block 202, so the sliding block 202 also has a through hole for the latch 204 to pass through. The number of the sockets 203 can be increased or decreased as needed, and when the position of the adjustment rod 209 needs to be adjusted forward and backward, the latch 204 can be pulled out, moved, and then inserted into another socket 203.

[0039] A positioning ring 206 is also provided on the side of the support column 207, and a first spring 205 is sleeved on the arc surface of the latch 204, and the two ends of the first spring 205 are respectively fixedly connected to the latch 204 and the positioning ring 206. The main function of the positioning ring 206 is that when the latch 204 is pulled out, the first spring 205 can connect the latch 204 and the positioning ring 206 together to prevent the latch 204 from being randomly placed or lost after being pulled out, and the hole on the positioning ring 206 is aligned with the through hole on the sliding block 202, which is convenient for the insertion of the latch 204.

[0040] The inner wall of the latch 204 is rotatably connected with a pull ring 229, and the latch 204 can be pulled by the pull ring 229, which is convenient for improving the speed and efficiency of the sampling operation. The sliding block 202 is a titanium alloy block with a hard surface. Its surface is less likely to deform after long-term use and has a long service life.

[0041] A locking mechanism is provided at one end of the arc surface of the adjusting rod 209 away from the motor 208. The locking mechanism includes a rotating block 210 fixedly connected to one end of the adjusting rod 209. Three slots 211 are provided on the side of the rotating block 210 away from the support column 207. The inner wall of one of the slots 211 is slidably connected to a limit block 213. The first electric push rod 212 is fixedly connected to another support column 207, and the output end of the first electric push rod 212 is fixedly connected to the limit block 213. When the position of the adjusting rod 209 is adjusted to the right position, the first electric push rod 212 is started, so that the limit block 213 connected to the output end of the first electric push rod 212 extends out and is stuck in one of the slots 211, thereby fixing the adjusting rod 209 to prevent sliding or rotation.

[0042] The cross section of the limiting block 213 is arc-shaped. When the first electric push rod 212 drives the limiting block 213 to contact the slot 211 , the arc-shaped limiting block 213 has better contact stability with the slot 211 and is easy to insert into the slot 211 .

[0043] A sampling grabbing mechanism is provided in the middle of the arc surface of the adjusting rod 209. The sampling grabbing mechanism includes a fixing plate 214 fixedly connected to the middle of the adjusting rod 209. A second electric push rod 215 is provided on the upper side of the fixing plate 214. A connecting block 216 is fixedly connected to the output end of the second electric push rod 215. A sampling sleeve 217 is provided on the lower side of the connecting block 216. The sampling sleeve 217 and the connecting block 216 are detachable. The interior of the sampling sleeve 217 is a hollow structure for food sampling. Fig.10 As shown, the inner top wall of the sampling sleeve 217 is fixedly connected to a second spring 218, and the lower end of the second spring 218 is fixedly connected to a pressing rod 219. The pressing rod 219 slides with the inner wall of the sampling sleeve 217 and penetrates through the side wall of the sampling sleeve 217. A slot is provided at a position corresponding to the pressing rod 219. When sampling, the straw container containing the sample is placed inside the sampling sleeve 217, the sampling grabbing mechanism is aligned with the food position, the second electric push rod 215 is started, the sampling sleeve 217 is driven downward and placed inside the food, and after the food to be tested enters the sampling sleeve 217, the second electric push rod 215 is started to return the sampling grabbing mechanism. The pressing rod 219 is pulled downward, and the tube filled with sampled food can be taken out of the sampling sleeve 217 by pressing the pressing rod 219.

[0044] Continue to refer Figure 3As shown, the placement plate 5 is provided with a container fixing mechanism, which has a fixed clamp 226 fixed on the placement plate 5 and an adjustment clamp 222 that can slide on the placement plate 5. A sliding groove 220 is provided on the placement plate 5, and a sliding rod 221 is provided at the bottom of the adjustment clamp 222. The lower end of the sliding rod 221 is placed in the sliding groove 220 and can slide back and forth in the sliding groove 220, thereby driving the adjustment clamp 222 to move toward or away from the fixed clamp 226. The adjustment clamp 222 and the fixed clamp 226 are both semicircular structures, which work together to fix the container containing food for easy sampling.

[0045] Adjust the side end of the clamp 222, that is, Figure 3 Two connecting rings 223 are provided at the right end of the adjusting clamp 222, one of which is fixedly connected to the adjusting clamp 222, and the other connecting ring 223 is fixedly connected to the fixing clamp 226. Figure 3 Two fixing rings 224 are provided at the left end, one of which is fixedly connected to the adjusting clamp 222, and the other is fixedly connected to the fixing clamp 226. The inner walls of the two connecting rings 223 are penetrated with a same first rotating rod 227, the first rotating rod 227 is threadedly connected to the connecting ring 223 on the adjusting clamp 222, the first rotating rod 227 is rotatably connected to the connecting ring 223 on the fixing clamp 226, and the inner walls of the two fixing rings 224 are slidably penetrated with a same guide rod 225.

[0046] One end of the first rotating rod 227 is fixedly connected to a rotating disk 228 , and the cross section of the rotating disk 228 is circular. When the first rotating rod 227 needs to be rotated, the first rotating rod 227 can be rotated through the rotating disk 228 , which is convenient for operation and improves efficiency.

[0047] The effect achieved by the entire sampling mechanism 2 is that when sampling various types of food, the sampling mechanism 2 can improve the efficiency of food sampling and the accuracy of subsequent food testing, and can also sample multiple locations of the same food by moving forward, backward, left and right, thereby improving the convenience of sampling other parts of the item. Moreover, the sampling mechanism is also suitable for sampling various forms of food, including liquid food, fixed food, and viscous gelatinous food.

[0048] like Figure 6 and Figure 7As shown, the storage device 3 includes a fixed frame 31, which is fixedly connected to the bottom plate 1. A storage groove 32 is provided on one side of the fixed frame 31. A storage box 35 is slidably connected to the inner wall of the storage groove 32. Three magnetic blocks 38 are fixedly connected to one side of the storage box 35. A metal plate 37 is adsorbed on the side of the three magnetic blocks 38 away from the storage box 35. The metal plate 37 is fixedly connected to one side of the inner wall of the storage groove 32. Four fixed rods 33 are fixedly connected to one end of the fixed frame 31 away from the bottom plate 1. One end of the four fixed rods 33 away from the arc surface of the fixed frame 31 is fixedly connected to the placement plate 5. A plurality of guide holes 34 are provided on the side of the placement plate 5. The plurality of guide holes 34 are evenly distributed on the placement plate 5. When sampling food through the sampling mechanism 2, the juice flowing out of the food sampling can be collected by the storage device 3, so as to avoid the juice flowing around and affecting the working environment of the entire sampling mechanism 2 by collecting. The storage box 35 is fixed on one side away from the magnetic block 38. A handle 36 is connected. When the storage box 35 needs to be pulled, the storage box 35 can be moved by the handle 36 installed on the storage box 35, so that the efficiency of the movement of the storage box 35 can be improved through the handle 36. A plurality of anti-slip grooves 39 are provided on both sides of the handle 36, and the plurality of anti-slip grooves 39 are evenly distributed on one side of the handle 36. When the storage box 35 is moved by the handle 36, the anti-slip grooves 39 provided on the handle 36 can increase the friction between the handle 36 and the user's hand, thereby improving the stability when the handle 36 is used.

[0049] The effect achieved by the entire storage device 3 is that when the food is sampled by the sampling mechanism 2, the overflowing juice from the food sampling can be collected by the storage device 3, thereby preventing the juice from affecting the environment of the entire sampling mechanism 2 through collection.

[0050] like Figure 8 and Fig. 9 As shown, the contact device 4 includes a contact plate 41, which is slidably connected to the base plate 1, and two assembly blocks 42 are fixedly connected on both sides of the contact plate 41. Storage grooves 43 are provided at the positions of the contact plate 41 corresponding to the four assembly blocks 42. The inner wall thread of the assembly block 42 is penetrated by a second rotating rod 45, and the second rotating rod 45 is threadedly connected to the inner wall of the base plate 1. When the base plate 1 contacts the desktop, the contact device 4 can be installed on the base plate 1. The contact device 4 can improve the stability of the base plate 1 when contacting the desktop, and avoid the small friction between the base plate 1 and the desktop, which affects the stability of the base plate 1 placed on the desktop. A guide block 44 is fixedly connected to one end of the arc surface of the second rotating rod 45 close to the base plate 1, and the guide block 44 is funnel-shaped. When the second rotating rod 45 is connected to the inner wall of the assembly block 42, the guide block 44 installed on the second rotating rod 45 can improve the connection efficiency between the second rotating rod 45 and the assembly block 42, so that the contact plate 41 can be fixed on the base plate 1 more quickly.

[0051] The effect achieved by the entire contact device 4 is that when the base plate 1 is placed on the desktop, the contact device 4 can be installed at the bottom of the base plate 1, so that the friction between the bottom of the base plate 1 and the desktop can be increased through the contact device 4, thereby improving the overall sampling stability and reducing the possibility of the base plate 1 sliding on the desktop.

[0052] The overall working principle is that when the food is placed on the placement plate 5, the first rotating rod 227 can be rotated on the connecting ring 223. The rotation of the first rotating rod 227 will drive the adjustment clamp 222 to move. The movement of the adjustment clamp 222 will move along the direction of the guide rod 225 through the fixing ring 224, toward the direction of the fixing clamp 226, so that the food can be fixed before sampling by the adjusting clamp 222 and the fixing clamp 226.

[0053] At this time, the second electric push rod 215 can be started, and the movement of the second electric push rod 215 will drive the connecting block 216 to move, so that the sampling sleeve 217 can be driven by the connecting block 216 to perform auxiliary sampling; when it is necessary to sample other parts of the food, the pin 204 is pulled out from the sliding block 202 and the base 201. At this time, the first spring 205 is in a stretched state, and the fixing effect of the sliding block 202 can be released, and the sliding block 202 can be adjusted to a suitable position. The pin 204 is released, and the first spring 205 is reset to drive the pin 204 to be fixedly connected with the sliding block 202 and the base 201 through the socket 203, so that the sliding block 202 can be fixed on the base 201.

[0054] The motor 208 drives the adjusting rod 209 to rotate, and the sampling angle can be adjusted by rotating the adjusting rod 209. The rotation of the adjusting rod 209 drives the rotating block 210 to move. After the adjusting rod 209 is adjusted to a suitable position, the first electric push rod 212 can be started, and the first electric push rod 212 drives the limit block 213 to connect with the card slot 211, so that the adjusted adjusting rod 209 can be fixed. When the first rotating rod 227 needs to be rotated, it can be operated through the rotating disk 228 installed on the first rotating rod 227. When the latch 204 needs to be pulled, it can be operated through the pull ring 229 installed on the latch 204. When the sliding block 202 made of titanium alloy is in use, its surface is relatively hard, and its surface is less likely to deform after long-term use, and its service life is relatively good. When the first electric push rod 212 drives the limit block 213 to contact the slot 211, the arc-shaped limit block 213 has better contact stability with the slot 211, and the arc-shaped limit block 213 can improve the contact effect when contacting the slot 211.

[0055] The storage box 35 is pushed into the fixed frame 31 through the storage slot 32, and the magnetic block 38 installed on the storage box 35 is brought into contact with the metal plate 37 installed at the bottom of the storage slot 32. The magnetic block 38 and the metal plate 37 can be adsorbed when they come into contact, and the storage box 35 can be fixed in the storage slot 32. At this time, when sampling food, overflowing juice will flow into the interior of the fixed frame 31 through the guide hole 34, so that the juice is collected by the storage box 35. When the storage box 35 needs to be pulled, the storage box 35 can be moved by the handle 36 installed on the storage box 35, so that the efficiency of the movement of the storage box 35 can be improved by the handle 36. When the storage box 35 is moved by the handle 36, the anti-slip groove 39 provided on the handle 36 can increase the friction between the handle 36 and the user's hand, thereby improving the stability when the handle 36 is used.

[0056] The contact plate 41 is brought into contact with the bottom of the base plate 1, and the second rotating rod 45 is rotated in the assembly block 42. The second rotating rod 45 will be connected to the inner wall of the base plate 1, and the second rotating rod 45 is continued to be rotated until the second rotating rod 45 is completely in the storage groove 43. The rotation of the second rotating rod 45 can be stopped, and the contact plate 41 can be fixed to the bottom of the base plate 1. When the second rotating rod 45 is connected to the inner wall of the assembly block 42, the guide block 44 installed on the second rotating rod 45 can improve the connection efficiency between the second rotating rod 45 and the assembly block 42, so that the contact plate 41 can be fixed to the base plate 1 more quickly.

[0057] When sampling and testing: 1) Place the food to be sampled on the placement plate 5, fix the container, and use the sampling grabbing mechanism to take multiple samples at different positions of the food for standby use; 2) Amplify the pathogenic bacteria in the sampled food through non-specific culture; 3) Perform direct specific real-time quantitative PCR or DNA extraction on the samples obtained by real-time quantitative sampling in step 2), and then perform specific real-time quantitative PCR identification and analysis on the extracted DNA.

[0058] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. An efficient sampling device for detecting pathogenic bacteria in food, comprising a base plate (1) and a sampling mechanism (2), characterized in that: A placement plate (5) and a sampling mechanism (2) are provided on the bottom plate (1), the sampling mechanism (2) comprising a sampling grabbing mechanism, an adjustment rod (209) and a locking mechanism, and two bases (201), both of which are fixedly connected to the bottom plate (1). The inner wall of the base (201) is slidably connected to a sliding block (202), the upper side of the sliding block (202) is fixedly connected to a support column (207), one side of one of the support columns (207) is fixedly connected to a motor (208), the output end of the motor (208) is fixedly connected to an adjustment rod (209), the arc surface of the adjustment rod (209) is rotatably penetrated by the inner wall of another support column (207), the sampling grabbing mechanism is arranged in the middle of the adjustment rod, and the locking mechanism is arranged at the end of the adjustment rod; The locking mechanism comprises a rotating block (210) fixedly connected to one end of the adjusting rod (209); three slots (211) are provided on a side of the rotating block (210) away from the supporting column (207); an inner wall of one of the slots (211) is slidably connected to a limiting block (213); a first electric push rod (212) is provided on one side of the limiting block (213); the first electric push rod (212) is fixedly connected to another supporting column (207); and an output end of the first electric push rod (212) is fixedly connected to the limiting block (213); The sampling and grabbing mechanism comprises a fixing plate (214) disposed on the adjusting rod (209), a second electric push rod (215) being fixedly connected to the upper side of the fixing plate (214), a connecting block (216) being fixedly connected to the output end of the second electric push rod (215), and a sampling sleeve (217) being fixedly connected to the output end of the connecting block (216) away from the second electric push rod (215). The placement plate is provided with a container fixing mechanism, the container fixing mechanism comprising a fixing clamp (226) fixed on the placement plate (5), and an adjusting clamp (222) slidable on the placement plate. A sliding groove (220) is provided on the upper side of the placement plate (5), and a sliding rod (221) slidable in the sliding groove is provided at the bottom of the adjusting clamp (222).

2. The efficient sampling device for detecting pathogenic bacteria in food according to claim 1, characterized in that: Two connecting rings (223) are provided at one end of the side surface of the adjusting clamp (222), one of the connecting rings (223) is fixedly connected to the adjusting clamp (222), and the other connecting ring (223) is fixedly connected to the fixing clamp (226); two fixing rings (224) are provided at the other end of the side surface of the adjusting clamp (222), one of the fixing rings (224) is fixedly connected to the adjusting clamp (222), and the other fixing ring (224) is fixedly connected to the fixing clamp (226); the inner walls of the two connecting rings (223) are provided with a same first rotating rod (227); the first rotating rod (227) is threadedly connected to one of the connecting rings (223); the first rotating rod (227) is rotatably connected to the other connecting ring (223); the inner walls of the two fixing rings (224) are slidably penetrated by a same guide rod (225); and a rotating disk (228) is fixedly connected to one end of the circular arc surface of the first rotating rod (227).

3. The efficient sampling device for detecting pathogenic bacteria in food according to claim 1, characterized in that: The side of the base (201) is provided with three insertion holes (203), the inner wall of one of the insertion holes (203) is slidably penetrated by a latch (204), the latch (204) and the inner wall of the sliding block (202) are slidably penetrated, the arc surface of the latch (204) is slidably connected to a positioning ring (206), the arc surface of the latch (204) is sleeved with a first spring (205), the two ends of the first spring (205) are respectively fixedly connected to the latch (204) and the positioning ring (206), the upper end of the positioning ring (206) is fixedly connected to the support column (207), and the inner wall of the latch (204) is rotatably connected to a pull ring (229).

4. The efficient sampling device for detecting pathogenic bacteria in food according to claim 1, characterized in that: The top wall of the sampling sleeve (217) is fixedly connected to a second spring (218), and one end of the second spring (218) away from the fixed plate (214) is fixedly connected to a pressing rod (219). The pressing rod (219) slides through the inner wall of the sampling sleeve (217), and the pressing rod can move up and down.

5. The efficient sampling device for detecting pathogenic bacteria in food according to claim 1, characterized in that: The bottom plate (1) is provided with a storage device (3), the storage device (3) comprising a fixed frame (31), the fixed frame (31) being fixedly connected to the bottom plate (1), a storage groove (32) being provided on one side of the fixed frame (31), a storage box (35) being slidably connected to the inner wall of the storage groove (32), three magnetic blocks (38) being fixedly connected to one side of the storage box (35), a metal plate (37) being adsorbed on the side of the three magnetic blocks (38) away from the storage box (35), the metal plate (37) being fixedly connected to one side of the inner wall of the storage groove (32), four fixed rods (33) being fixedly connected to one end of the fixed frame (31) away from the bottom plate (1), the four fixed rods (33) being fixedly connected to the placement plate (5) at one end of the circular arc surface away from the fixed frame (31), a plurality of guide holes (34) being provided on the side of the placement plate (5), the plurality of guide holes (34) being evenly distributed on the placement plate (5).

6. The efficient sampling device for detecting pathogenic bacteria in food according to claim 5, characterized in that: A handle (36) is fixedly connected to a side of the storage box (35) away from the magnetic block (38).

7. The efficient sampling device for detecting pathogenic bacteria in food according to claim 6, characterized in that: A plurality of anti-slip grooves (39) are provided on both sides of the handle (36), and the plurality of anti-slip grooves (39) are evenly distributed on one side of the handle (36).

8. The efficient sampling device for detecting pathogenic bacteria in food according to claim 1, characterized in that: A contact device (4) is provided on a side of the bottom plate (1) away from the placement plate (5), the contact device (4) comprising a contact plate (41), the contact plate (41) being slidably connected to the bottom plate (1), two assembly blocks (42) being fixedly connected to both sides of the contact plate (41), storage grooves (43) being provided at positions of the contact plate (41) corresponding to the four assembly blocks (42), a second rotating rod (45) being threadedly penetrated through the inner wall of the assembly block (42), and the second rotating rod (45) being threadedly connected to the inner wall of the bottom plate (1).

9. The efficient sampling device for detecting pathogenic bacteria in food according to claim 8, characterized in that: One end of the arc surface of the second rotating rod (45) close to the bottom plate (1) is fixedly connected to a guide block (44), and the guide block (44) is funnel-shaped.

10. A sampling and detection method based on the efficient sampling device for detecting pathogenic bacteria in food according to any one of claims 1 to 9, characterized in that: The sampling and detection method comprises the following steps: 1) placing the food to be sampled on the placement plate (5), fixing the container, and taking multiple samples at different positions of the food by means of a sampling grabbing mechanism; 2) Amplify the pathogenic bacteria in the sampled food through non-specific culture; 3) Perform direct specific real-time quantitative PCR or DNA extraction on the samples obtained by real-time quantitative sampling in step 2), and then perform specific real-time quantitative PCR identification and analysis on the extracted DNA.

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