Human body substance content change history detection method and health history evaluation method

Abstract

The invention relates to the technical field of biochemistry, in particular to a human body substance content change history detection method and a health history evaluation method, and the detection method comprises the following steps: obtaining and processing a detection material; acquiring a spectrum of a detected material; calculating the abnormal time of the target object corresponding to the mass spectrum; and judging the abnormal duration of the target object. According to the method, the matrix-assisted laser ionization analysis time-of-flight mass spectrometer is used for carrying out spectrum collection on the detected material to determine the abnormal position of the target object, then the abnormal duration of the target object is calculated according to the abnormal position of the target object, the initial position of the detected material, the growth speed of the detected material and the time for obtaining the detected material, and then a relatively complete target object content change history is obtained. In addition, a relatively complete human health history is obtained according to the target object content change history, so that the purposes of preventing diseases and assisting doctors in diagnosis are achieved.

Description

Technical Field

[0001] The present invention relates to the field of biochemical technology, and in particular to a method for detecting the history of changes in human body substance content and a method for evaluating health history. Background Art

[0002] Currently, in physical examinations or medical treatments, the content of specific substances in the human body can be determined through testing, and whether it exceeds the standard or is lacking, thereby judging the health status of the human body, achieving the purpose of preventing diseases and assisting doctors in diagnosis.

[0003] For example, vitamins are a class of small molecular trace organic substances necessary to maintain normal physiological functions and human health. They are basically impossible to synthesize in the human body and must be obtained through food. If they are deficient, they will cause corresponding diseases. Through the detection of vitamins, we can get the relationship between vitamins and human health, provide favorable support for doctors' popular science education, and increase the attention to human vitamin monitoring to achieve early prevention, early detection, early treatment, and promote healthy growth of the human body.

[0004] For example, trace elements have an optimal concentration range in the human body. When they are insufficient or the concentration exceeds a certain limit, it may cause adverse effects on human health. The content of trace elements in hair is closely related to human health. In addition to age and gender, trace elements are also affected by factors such as eating habits, living environment, geographical location and other exogenous risks. The detection of trace elements can be used as effective data for evaluating occupational exposure to lead.

[0006] However, the existing technology is unable to detect the specific time and duration of abnormalities in human body substances, and therefore it is impossible to obtain a relatively complete history of changes in human body substance content, nor a relatively complete history of human health. Summary of the invention

[0007] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or be understood by implementing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures particularly pointed out in the description and other drawings of the description.

[0008] The purpose of the present invention is to overcome the above-mentioned shortcomings and provide a method for detecting the history of changes in human body substance content and a method for evaluating health history. The present invention uses a matrix-assisted laser ionization analysis time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object according to the abnormal position of the target object, the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample, so as to obtain a relatively complete history of changes in the content of the target object. In addition, the present invention obtains a relatively complete history of human health based on the history of changes in the content of the target object, so as to achieve the purpose of preventing diseases and assisting doctors in diagnosis.

[0009] To achieve the above object, the technical solution of the present invention is: a method for detecting the change history of human body substance content, comprising the following steps:

[0010] Acquisition and processing of test materials: acquisition and processing of test materials;

[0011] The sample is collected by using a matrix-assisted laser ionization-desorption time-of-flight mass spectrometer to collect the spectrum of the processed sample to form a mass spectrum. The position of the target object's peak in the mass spectrum is used as the basis for the detection of the target object;

[0012] Calculation of the abnormal time of the target object corresponding to the mass spectrum. Determine the abnormal position of the target object according to the position of the abnormal mass spectrum peak in the sample. Calculate the abnormal time of the target object according to the distance from the abnormal position of the target object to the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample.

[0013] The duration of the target object's abnormality is determined based on the time when the target object is abnormal and the time when the sample is obtained.

[0014] The present invention uses a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object based on the abnormal position of the target object, the starting position of the sample, the growth rate of the sample and the time of obtaining the sample, thereby obtaining a relatively complete history of changes in the target object content.

[0015] In some embodiments, the calculation formula for the target object abnormality duration is:

[0016] T x =S x / V………………(1)

[0017] T=T o -T x ………………(2)

[0018] Among them, S x is the distance between the abnormal position of the target object and the starting position of the sample, V is the growth rate of the sample, T x is the time when the target object is abnormal, T o is the time to obtain the sample, and T is the abnormal duration of the target object.

[0019] In some embodiments, the sample is a hair sample or a nail sample. The present invention uses hair or nails as the sample, which has the following advantages: the acquisition method and time of hair and nails are convenient and can be done at any time; hair and nails have time traceability; and the sampling of hair and nails is less destructive.

[0020] In some embodiments, the pretreatment of the hair sample includes

[0021] Cleaning: ultrasonically clean the obtained hair samples with deionized water and acetone respectively, repeat 2-5 times and then dry them;

[0022] Crush and explode the cleaned and dried hair samples.

[0023] The present invention performs pre-processing on the hair sample, which can reduce the influence of the doping substances to the minimum and greatly improve the detection accuracy of the hair sample.

[0024] In some embodiments, the compression explosion specifically includes placing the cleaned and dried hair sample in the middle of a folded weighing paper, placing the weighing paper between two flat film tools, and using a tablet press to perform compression explosion for 0s-10s, and finally attaching the compressed hair sample fragments to a sample plate with conductive glue for detection. The present invention performs compression explosion on the cleaned and dried hair sample sample, so that the target object in the hair sample sample can be completely exposed, which is convenient for the detection of the target object and provides a better guarantee for the accuracy and precision of subsequent detection data.

[0025] In some embodiments, the pressure of the tablet press is 0 MPa-30 MPa. The present invention can fully expose the target object in the hair sample by reasonably controlling the pressure during the compression explosion process, and can also ensure its integrity.

[0026] In some embodiments, the conductive adhesive is a double-sided carbon conductive adhesive with a resistivity of less than 5 ohms / mm. 2 Double-sided carbon conductive adhesive has good conductivity and is specially designed for fixed observation samples. It can greatly reduce background interference, not only improve observation accuracy but also reduce observation limits, which is conducive to enhancing the ionization of target objects in the test samples.

[0027] The present invention also provides a health history assessment method based on the history of changes in human body substance content, comprising the following steps:

[0028] Acquisition and processing of test materials: acquisition and processing of test materials;

[0029] The sample is collected by using a matrix-assisted laser ionization-desorption time-of-flight mass spectrometer to collect the spectrum of the processed sample to form a mass spectrum. The position of the target object's peak in the mass spectrum is used as the basis for the detection of the target object;

[0030] Calculation of the abnormal time of the target object corresponding to the mass spectrum. Determine the abnormal position of the target object according to the position of the abnormal mass spectrum peak in the sample. Calculate the abnormal time of the target object according to the distance from the abnormal position of the target object to the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample.

[0031] Determine the duration of the target object's abnormality based on the time when the target object is abnormal and the time when the sample is obtained;

[0032] The generation of human health points is based on the target content and / or the abnormal duration of the target.

[0033] The present invention first uses a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object according to the abnormal position of the target object, the starting position of the sample, the growth rate of the sample and the time of obtaining the sample, thereby obtaining a relatively complete target content change history, and then based on the target content change history, the target content and / or the target abnormal duration are obtained to generate a health point, which can be used as a reference for disease prevention and doctor diagnosis.

[0034] In some embodiments, a health point is generated based on the target content and / or the abnormal duration of the target, including:

[0035] If the target content is within the preset content range, a healthy point is generated and the healthy point is determined to be a first color (for example, green);

[0036] If the target object content is outside the preset content range, a healthy point is generated, and the healthy point is determined to be a second color (eg, red).

[0037] The present invention extracts the target content from the target content change history, and determines whether the target content is within a preset content range, and finally generates a colored health point to prevent diseases and assist doctors in disease diagnosis.

[0038] In some embodiments, a health point is generated based on the target content and / or the abnormal duration of the target, including:

[0039] If the target content is within the preset content range, a healthy point is generated and the healthy point is determined to be a first color (for example, green);

[0040] If the target object content is outside the preset content range, and the target object abnormality duration is less than or equal to the preset duration, a healthy point is generated, and the healthy point is determined to be a third color (for example, light red);

[0041] If the target object content is outside the preset content range, and the target object abnormality duration is longer than the preset duration, a healthy point is generated, and the healthy point is determined to be a fourth color (eg, dark red).

[0042] The present invention extracts the target content and the abnormal duration of the target from the target content change history, and determines whether the target content is within the preset content range. Combined with the abnormal duration of the target, it finally generates a colored health point to prevent diseases and assist doctors in disease diagnosis.

[0043] By adopting the above technical solution, the beneficial effects of the present invention are:

[0044] The present invention uses a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object based on the abnormal position of the target object, the starting position of the sample, the growth rate of the sample and the time of obtaining the sample, thereby obtaining a relatively complete history of changes in the target object content.

[0045] The present invention obtains the target content and the target abnormality duration from the target content change history, determines whether the target content is within a preset content range and combines the target abnormality duration, thereby obtaining a more complete human health history, so as to achieve the purpose of preventing diseases and assisting doctors in diagnosis.

[0046] The sample of the present invention is non-invasive, not only is sampling easy and repeatable, but also the processing method is simple, the loss of the target object during the extraction process is small, and the processing time is greatly shortened.

[0047] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present disclosure.

[0048] Undoubtedly, these and other objects of the present invention will become more apparent after the following detailed description of the preferred embodiment described with reference to various figures and drawings.

[0049] In order to make the above and other purposes, features and advantages of the present invention more obvious and understandable, one or several preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation of the present invention.

[0051] In the drawings, the same reference numerals are used for the same components and the drawings are schematic and not necessarily drawn to scale.

[0052] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only one or several embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on such drawings without paying creative work.

[0053] Figure 1 A flow chart for detecting the substance content change history of some embodiments of the present invention;

[0054] Figure 2 A flow chart of hair sample preprocessing in some embodiments of the present invention;

[0055] Figure 3 A detection principle diagram of a matrix-assisted laser ionization-desorption time-of-flight mass spectrometer according to some embodiments of the present invention;

[0056] Figure 4 This is a picture showing the effect of the hair sample before being processed;

[0057] Figure 5 This is a diagram showing the effect of the hair sample after slicing;

[0058] Figure 6 This is a diagram showing the effect of the hair sample after being compressed and exploded;

[0059] Figure 7 This is a diagram showing the effect of the hair sample after dissolution of the present invention;

[0060] Figure 8 This is a picture showing the effect of hair sample after perming;

[0061] Fig. 9 This is a flow chart of human health history assessment based on the history of changes in substance content according to some embodiments of the present invention. DETAILED DESCRIPTION

[0062] The following will describe the implementation methods of the present invention in detail with reference to the accompanying drawings and embodiments, so that the implementation process of how the present invention applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly. It should be noted that as long as there is no conflict, the various embodiments of the present invention and the various features in the embodiments can be combined with each other, and the technical solutions formed are all within the protection scope of the present invention.

[0063] Meanwhile, in the following description, many specific details are set forth for the purpose of explanation to provide a thorough understanding of the embodiments of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without the specific details or the specific manner described herein.

[0064] Reference Figure 1-3 , Figure 1 A flow chart for detecting the substance content change history of some embodiments of the present invention; Figure 2 A flow chart of hair sample preprocessing in some embodiments of the present invention; Figure 3 Schematic diagram of the detection principle of a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer according to some embodiments of the present invention.

[0065] According to some embodiments of the present invention, the present invention provides a method for detecting the history of changes in human body substance content. The detection method comprises the following steps: acquisition and processing of sample, spectrum collection of sample, calculation of the abnormal time of the target object corresponding to the mass spectrum, and determination of the abnormal duration of the target object.

[0066] The acquisition and processing of test materials specifically include: acquiring test materials and processing them;

[0067] The specific method of sampling the sample is as follows: the sample after treatment is sampled by matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to form a mass spectrum, and the position of the target peak in the mass spectrum is used as the basis for detecting the target;

[0068] The calculation of the abnormal time of the target object corresponding to the mass spectrum is specifically as follows: the abnormal position of the target object is determined according to the position of the abnormal mass spectrum peak in the sample, and the abnormal time of the target object is calculated according to the distance from the abnormal position of the target object to the starting position of the sample, the growth rate of the sample and the time of obtaining the sample;

[0069] The determination of the duration of the target object being abnormal is specifically as follows: the duration of the target object being abnormal is determined based on the time when the target object is abnormal and the time when the sample is obtained.

[0070] The present invention uses a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object based on the abnormal position of the target object, the starting position of the sample, the growth rate of the sample and the time of obtaining the sample, thereby obtaining a relatively complete history of changes in the target object content.

[0071] According to some embodiments of the present invention, optionally, the calculation formula for the target object abnormality duration is:

[0072] T x =S x / V………………(1)

[0073] T=T o -T x ………………(2)

[0074] Among them, S x is the distance between the abnormal position of the target object and the starting position of the sample, V is the growth rate of the sample, T xis the time when the target object is abnormal, T o is the time to obtain the sample, and T is the abnormal duration of the target object.

[0075] According to some embodiments of the present invention, the sample is optionally a hair sample or a nail sample. The present invention uses hair or nails as the sample, which has the following advantages: the acquisition method and time of hair and nails are convenient and can be done at any time; hair and nails have time traceability; and hair and nail sampling is less destructive.

[0076] Optionally, the hair includes but is not limited to head hair, armpit hair, sweat hair or hair from other parts. The hair acquisition process is specifically as follows: cutting close to the hair root. The hair production speed is specifically as follows: normal hair growth speed: 0.27mm / day-0.4mm / day, normal armpit hair growth speed: 0.21mm / day-0.38mm / day, and hair growth speed of other parts: about 0.2mm / day.

[0077] Optionally, nails include but are not limited to fingernails and toenails. The specific process of obtaining nails is: if the part can be cut directly, it is cut from the tip of the nail; if the part cannot be cut directly, the nail powder is scraped according to the area, and the time is converted according to the distance between the sampling site and the growing end of the nail. The growth rate of nails is specifically: the growth rate of normal fingernails is about 3.5 mm / month, and the growth rate of normal toenails is about 1.6 mm / month.

[0078] According to some embodiments of the present invention, optionally, the pretreatment of the hair sample includes:

[0079] Cleaning: ultrasonically clean the obtained hair samples with deionized water and acetone respectively, repeat 2-5 times and then dry them;

[0080] Crush and explode the cleaned and dried hair samples.

[0081] The present invention performs pre-processing on the hair sample, which can reduce the influence of the doping substances to the minimum and greatly improve the detection accuracy of the hair sample.

[0082] According to some embodiments of the present invention, optionally, the AR of acetone is ≥ 99.5%.

[0083] According to some embodiments of the present invention, optionally, during ultrasonic cleaning, the power is 400W-900W and the temperature is 20° C.-45° C. The present invention can effectively remove impurities in the hair sample and effectively preserve the integrity of the hair sample by reasonably setting the power and temperature during ultrasonic cleaning.

[0084] Reference Figure 4-8 , Figure 4 This is a picture showing the effect of the hair sample before being processed; Figure 5 This is a diagram showing the effect of the hair sample after slicing; Figure 6 This is a diagram showing the effect of the hair sample after being compressed and exploded; Figure 7 This is a diagram showing the effect of the hair sample after dissolution of the present invention; Figure 8 This is a diagram showing the effect of hair samples after perming.

[0085] According to some embodiments of the present invention, optionally, the compression explosion specifically includes placing the cleaned and dried hair sample in the middle of a folded weighing paper, placing the weighing paper between two flat film tools, and using a tablet press to perform compression explosion for 0s-10s, and finally attaching the compressed hair sample fragments to a sample plate with conductive glue for detection. The present invention performs compression explosion on the cleaned and dried hair sample sample, so that the target object in the hair sample sample can be completely exposed, which is convenient for the detection of the target object and provides a better guarantee for the accuracy and precision of subsequent detection data.

[0086] According to some embodiments of the present invention, the pressure of the tablet press is optionally 0MPa-30MPa. The present invention can fully expose the target object in the hair sample by reasonably controlling the pressure during the compression explosion process, and can also ensure its integrity.

[0087] According to some embodiments of the present invention, optionally, the conductive adhesive is a double-sided carbon conductive adhesive with a resistivity of <5 ohms / mm 2 Double-sided carbon conductive adhesive has good conductivity and is specially designed for fixed observation samples. It can greatly reduce background interference, not only improve observation accuracy but also reduce observation limits, which is conducive to enhancing the ionization of target objects in the test samples.

[0088] According to some embodiments of the present invention, optionally, the pretreatment of the hair sample includes:

[0089] Cleaning: ultrasonically clean the obtained hair samples with deionized water and acetone respectively, repeat 2-5 times and then dry them;

[0090] Slice the cleaned and dried hair sample.

[0091] According to some embodiments of the present invention, the slicing is specifically as follows: the cleaned and dried hair sample is divided into 1cm-5cm hair sample segments, the hair sample segments are then cut into two halves so that the center of the hair sample segments is evenly exposed, and finally the cut hair sample segments are placed on a sample plate with conductive glue for detection. The present invention divides and slices the cleaned and dried hair sample so that the target object in the hair sample can be easily decomposed and extracted.

[0092] According to some embodiments of the present invention, optionally, the pretreatment of the hair sample includes:

[0093] Cleaning: ultrasonically clean the obtained hair samples with deionized water and acetone respectively, repeat 2-5 times and then dry them;

[0094] Dissolving: Dissolving the washed and dried hair sample.

[0095] According to some embodiments of the present invention, optionally, the dissolution is specifically as follows: align the starting positions of the washed and dried hair samples, cut them into hair sample fragments in units of 1 cm according to the growth direction, and then place the hair sample fragments in a 2 ml cryo-grinding tube, and add appropriate amounts of ceramic beads, 0.1 mL-20 mL of methanol extract containing methoxyphenamine in sequence, and place them in a cryo-grinding machine system for cryo-grinding (the cryo-grinding here is specifically: one round of 3.5 minutes, 3 rounds of grinding, and the temperature is around 0°C. Grinding will heat up, and liquid nitrogen can be used to control the temperature), and finally take 50 μL-200 μL of supernatant for detection. The present invention decomposes and extracts the target object in the hair sample by cryo-grinding, and performs it under low temperature conditions, which will not damage the properties of the target object itself and affect the accuracy and precision of subsequent test data.

[0096] According to some embodiments of the present invention, optionally, the content of methoxyphenamine in the methanol extract is 0.1 mg / mL-20 mg / mL.

[0097] According to some embodiments of the present invention, optionally, the particle size of the ceramic beads is 1 mm-5 mm, and the amount of ceramic beads added is 18-22. In the cryogenic grinding, the addition of ceramic beads greatly increases the friction between the hair sample and the ceramic beads, so that the hair sample can be fully ground to ensure that the target object in the hair sample can be fully decomposed and extracted.

[0098] According to some embodiments of the present invention, optionally, the pretreatment of the hair sample includes:

[0099] Cleaning: ultrasonically clean the obtained hair samples with deionized water and acetone respectively, repeat 2-5 times and then dry them;

[0100] Perm-lysis: the washed and dried hair samples are permed.

[0101] According to some embodiments of the present invention, optionally, the calding process specifically comprises: wrapping and fixing the washed and dried hair sample with aluminum foil from the starting position, taking 1 cm as a hair sample segment and placing it on a ceramic heating plate and heating it for 1 min-10 min to wait for detection.

[0102] Reference Fig. 9 , Fig. 9 This is a flow chart of human health history assessment based on the history of changes in substance content according to some embodiments of the present invention.

[0103] According to some embodiments of the present invention, the present invention further provides a health history assessment method based on the history of changes in human body substance content, comprising the following steps:

[0104] Acquisition and processing of test materials: acquisition and processing of test materials;

[0105] The sample is collected by using a matrix-assisted laser ionization-desorption time-of-flight mass spectrometer to collect the spectrum of the processed sample to form a mass spectrum. The position of the target object's peak in the mass spectrum is used as the basis for the detection of the target object;

[0106] Calculation of the abnormal time of the target object corresponding to the mass spectrum. Determine the abnormal position of the target object according to the position of the abnormal mass spectrum peak in the sample. Calculate the abnormal time of the target object according to the distance from the abnormal position of the target object to the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample.

[0107] Determine the duration of the target object's abnormality based on the time when the target object is abnormal and the time when the sample is obtained;

[0108] The generation of human health points is based on the target content and / or the abnormal duration of the target.

[0109] The present invention first uses a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object according to the abnormal position of the target object, the starting position of the sample, the growth rate of the sample and the time of obtaining the sample, thereby obtaining a relatively complete target content change history, and then based on the target content change history, the target content and / or the target abnormal duration are obtained to generate a health point, which can be used as a reference for disease prevention and doctor diagnosis.

[0110] According to some embodiments of the present invention, optionally, a health point is generated according to the target object content and / or the target object abnormality duration, including:

[0111] If the target content is within the preset content range, a healthy point is generated and the healthy point is determined to be a first color (for example, green);

[0112] If the target object content is outside the preset content range, a healthy point is generated, and the healthy point is determined to be a second color (eg, red).

[0113] The present invention extracts the target content from the target content change history, and determines whether the target content is within a preset content range, and finally generates a colored health point to prevent diseases and assist doctors in disease diagnosis.

[0114] Table 1 A detailed list of health points generated based on the content of a certain target substance in the human body within 10 months

[0115]

[0116] As can be seen from Table 1, the present invention generates a plurality of colored health points by detecting the content change of a certain target object in the human body within a certain period of time and judging whether the content is within a preset content range.

[0117] According to some embodiments of the present invention, optionally, a health point is generated according to the target object content and / or the target object abnormality duration, including:

[0118] If the target content is within the preset content range, a healthy point is generated and the healthy point is determined to be a first color (for example, green);

[0119] If the target object content is outside the preset content range, and the target object abnormality duration is less than or equal to the preset duration, a healthy point is generated, and the healthy point is determined to be a third color (for example, light red);

[0120] If the target object content is outside the preset content range, and the target object abnormality duration is longer than the preset duration, a healthy point is generated, and the healthy point is determined to be a fourth color (eg, dark red).

[0121] The present invention extracts the target content and the abnormal duration of the target from the target content change history, and determines whether the target content is within the preset content range. Combined with the abnormal duration of the target, it finally generates a colored health point to prevent diseases and assist doctors in disease diagnosis.

[0122] Table 2 A detailed list of health points generated based on the content of a target substance in the human body within 10 months and the duration of abnormality

[0123]

[0124] As can be seen from Table 2, the present invention detects the content change of a certain target object in the human body within a certain period of time, determines whether its content is within the pre-examination content range, and combines the abnormal duration to generate multiple colored health points.

[0125] Exemplary Detection Methods

[0126] This exemplary detection method is a method for detecting the change history of human body substance content, which includes the following steps:

[0127] Step S1: Acquisition and processing of hair samples, obtaining hair samples and processing the hair samples.

[0128] In this exemplary detection method, hair includes but is not limited to head hair, armpit hair, sweat hair or hair from other parts. The hair acquisition process is specifically: cutting close to the hair root. The hair production speed is specifically: normal hair growth speed: 0.27mm / day-0.4mm / day, normal armpit hair growth speed: 0.21mm / day-0.38mm / day, and hair growth speed in other parts: about 0.2mm / day.

[0129] In this exemplary detection method, the pretreatment of the hair sample includes cleaning and explosion. Among them, cleaning specifically involves ultrasonic cleaning the obtained hair sample with deionized water and acetone with AR≥99.5% in turn (power is 400W-900W, temperature is 20℃-45℃), repeating 2-5 times and then drying; explosion specifically involves placing the cleaned and dried hair sample in the middle of a folded weighing paper, and then placing the weighing paper between two flat membranes, using a sheet press (pressure is 0MPa-30Mpa) for explosion for 0s-10s, and finally sticking the exploded hair sample fragments on a sheet of conductive glue (which is double-sided carbon conductive glue with a resistivity of <5ohms / mm 2 The present invention compresses and explodes the cleaned and dried hair sample, so that the target object in the hair sample can be completely exposed, which is convenient for the detection of the target object and provides better guarantee for the accuracy and precision of subsequent detection data.

[0130] Step S2: Spectral sampling of the hair sample: a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer is used to sample the processed hair sample to form a mass spectrum, and the position of the target object's spectral peak in the mass spectrum is used as the basis for detecting the target object.

[0131] Step S3: Calculation of the time when the mass spectrum corresponds to the abnormality of the target object. The abnormal position of the target object is determined according to the position of the abnormal mass spectrum peak in the sample. The abnormal time of the target object is calculated according to the distance from the abnormal position of the target object to the starting position of the hair sample, the growth rate of the hair sample and the time of obtaining the hair sample.

[0132] In this exemplary detection method, the calculation formula for the time when the target object is abnormal is:

[0133] T x =S x / V………………(1)

[0134] Among them, S x is the distance between the abnormal position of the target object and the starting position of the sample, V is the growth rate of the sample, T x The time when the target object is abnormal.

[0135] Step S4: Determine the duration of the target object abnormality, based on the duration of the target object abnormality and the time of obtaining the hair sample, determine the duration of the target object abnormality.

[0136] In this exemplary detection method, the calculation formula for the target object abnormality duration is:

[0137] T=T o -T x ………………(2)

[0138] Among them, T x is the time when the target object is abnormal, T o is the time to obtain the sample, and T is the abnormal duration of the target object.

[0139] In this exemplary detection method, the present invention utilizes a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal location of the target object, and then calculates the abnormal duration of the target object based on the abnormal location of the target object, the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample, thereby obtaining a relatively complete history of changes in the target object content.

[0140] Exemplary Assessment Methods

[0141] The evaluation method of this embodiment is a human health history evaluation method based on the history of changes in substance content, which includes the following steps:

[0142] Step S1: Acquisition and processing of hair samples, obtaining hair samples and processing the hair samples.

[0143] In this exemplary detection method, hair includes but is not limited to head hair, armpit hair, sweat hair or hair from other parts. The hair acquisition process is specifically: cutting close to the hair root. The hair production speed is specifically: normal hair growth speed: 0.27mm / day-0.4mm / day, normal armpit hair growth speed: 0.21mm / day-0.38mm / day, and hair growth speed in other parts: about 0.2mm / day.

[0144] In this exemplary evaluation method, the pretreatment of the hair sample includes cleaning and explosion. Among them, cleaning specifically involves ultrasonic cleaning the obtained hair sample with deionized water and acetone with AR≥99.5% in turn (power is 400W-900W, temperature is 20℃-45℃), repeating 2-5 times and then drying; explosion specifically involves placing the cleaned and dried hair sample in the middle of a folded weighing paper, and then placing the weighing paper between two flat membranes, using a sheet press (pressure is 0MPa-30Mpa) for explosion for 0s-10s, and finally sticking the exploded hair sample fragments on a sheet of conductive glue (which is double-sided carbon conductive glue with a resistivity of <5ohms / mm 2The present invention compresses and explodes the cleaned and dried hair sample, so that the target object in the hair sample can be completely exposed, which is convenient for the detection of the target object and provides better guarantee for the accuracy and precision of subsequent detection data.

[0145] Step S2: Spectral sampling of the hair sample: a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer is used to sample the processed hair sample to form a mass spectrum, and the position of the target object's spectral peak in the mass spectrum is used as the basis for detecting the target object.

[0146] Step S3: Calculation of the time when the mass spectrum corresponds to the abnormality of the target object. The abnormal position of the target object is determined according to the position of the abnormal mass spectrum peak in the sample. The abnormal time of the target object is calculated according to the distance from the abnormal position of the target object to the starting position of the hair sample, the growth rate of the hair sample and the time of obtaining the hair sample.

[0147] In this exemplary evaluation method, the calculation formula for the time when the target object is abnormal is:

[0148] T x =S x / V………………(1)

[0149] Among them, S x is the distance between the abnormal position of the target object and the starting position of the sample, V is the growth rate of the sample, T x The time when the target object is abnormal.

[0150] Step S4: Determine the duration of the target object abnormality, based on the duration of the target object abnormality and the time of obtaining the hair sample, determine the duration of the target object abnormality.

[0151] In this exemplary evaluation method, the calculation formula for the target object abnormality duration is:

[0152] T=T o -T x ………………(2)

[0153] Among them, T x is the time when the target object is abnormal, T o is the time to obtain the sample, and T is the abnormal duration of the target object.

[0154] Step S4: generating a human health point, generating a health point according to the target object content and / or the target object abnormality duration.

[0155] In this exemplary evaluation method, a healthy point is generated based on the target object content and / or the target object abnormality duration, including if the target object content is within a preset content range, a healthy point is generated, and the healthy point is determined to be a first color (for example, green); if the target object content is outside the preset content range and the target object abnormality duration is less than or equal to the preset duration, a healthy point is generated, and the healthy point is determined to be a third color (for example, light red); if the target object content is outside the preset content range and the target object abnormality duration is greater than the preset duration, a healthy point is generated, and the healthy point is determined to be a fourth color (for example, dark red).

[0156] The present invention first uses a matrix-assisted laser ionization desorption / time-of-flight mass spectrometer to sample the sample to determine the abnormal position of the target object, and then calculates the abnormal duration of the target object according to the abnormal position of the target object, the starting position of the sample, the growth rate of the sample and the time of obtaining the sample, thereby obtaining a relatively complete target content change history, and then extracts the target content from the target content change history, and determines whether the target content is within a preset content range, combined with the abnormal duration of the target object, and finally generates a colored health point to prevent diseases and assist doctors in disease diagnosis.

[0157] It should be understood that the embodiments disclosed in the present invention are not limited to the specific processing steps or materials disclosed herein, but should be extended to equivalent substitutions of such features understood by ordinary technicians in the relevant field. It should also be understood that the terms used herein are only used for the purpose of describing specific embodiments and are not meant to be limiting.

[0158] The "embodiment" mentioned in the specification means that the specific features or characteristics described in conjunction with the embodiment are included in at least one embodiment of the present invention. Therefore, the phrases or "embodiment" appearing in various places throughout the specification do not necessarily refer to the same embodiment.

[0159] It should be noted that many specific details are set forth in the above description to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed above.

Claims

1. A method for detecting the change history of human body substance content, characterized in that: The following steps are involved: Acquisition and processing of test materials: acquisition and processing of test materials; The sample is collected by using a matrix-assisted laser ionization-desorption time-of-flight mass spectrometer to collect the spectrum of the processed sample to form a mass spectrum. The position of the target object's peak in the mass spectrum is used as the basis for the detection of the target object; Calculation of the abnormal time of the target object corresponding to the mass spectrum. Determine the abnormal position of the target object according to the position of the abnormal mass spectrum peak in the sample. Calculate the abnormal time of the target object according to the distance from the abnormal position of the target object to the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample. The duration of the target object's abnormality is determined based on the time when the target object is abnormal and the time when the sample is obtained.

2. The method for detecting the change history of human body substance content according to claim 1, characterized in that: The calculation formula for the target object abnormal duration is: T x =S x / V………………(1) T=T o -T x ………………(2) Among them, S x is the distance between the abnormal position of the target object and the starting position of the sample, V is the growth rate of the sample, T x is the time when the target object is abnormal, T o is the time to obtain the sample, and T is the abnormal duration of the target object.

3. The method for detecting the change history of human body substance content according to claim 1, characterized in that: The samples are hair samples or nail samples.

4. The method for detecting the change history of human body substance content according to claim 3, characterized in that: Hair sample pretreatment includes Cleaning: ultrasonically clean the obtained hair samples with deionized water and acetone respectively, repeat 2-5 times and then dry them; Crush and explode the cleaned and dried hair samples.

5. The method for detecting the change history of human body substance content according to claim 4, characterized in that: The specific method of explosion pressing is to place the cleaned and dried hair sample in the middle of a folded weighing paper, then place the weighing paper between two flat membranes, use a sheet press to explode the hair sample for 0s-10s, and finally stick the exploded hair sample fragments on a sample plate with conductive glue for testing.

6. The method for detecting the change history of human body substance content according to claim 5, characterized in that: The pressure of the tablet press is 0MPa-30Mpa.

7. The method for detecting the change history of human body substance content according to claim 5, characterized in that: The conductive adhesive is double-sided carbon conductive adhesive with a resistivity of <5ohms / mm 2 .

8. A health history assessment method based on the history of changes in human body substance content, characterized in that: The following steps are involved: Acquisition and processing of test materials: acquisition and processing of test materials; The sample is collected by using a matrix-assisted laser ionization-desorption time-of-flight mass spectrometer to collect the spectrum of the processed sample to form a mass spectrum. The position of the target object's peak in the mass spectrum is used as the basis for the detection of the target object; Calculation of the abnormal time of the target object corresponding to the mass spectrum. Determine the abnormal position of the target object according to the position of the abnormal mass spectrum peak in the sample. Calculate the abnormal time of the target object according to the distance from the abnormal position of the target object to the starting position of the sample, the growth rate of the sample, and the time of obtaining the sample. Determine the duration of the target object's abnormality based on the time when the target object is abnormal and the time when the sample is obtained; The generation of human health points is based on the target content and / or the abnormal duration of the target.

9. The health history assessment method based on the history of changes in human body substance content according to claim 8, characterized in that: Generate health points based on the target content and / or target abnormality duration, including If the target object content is within the preset content range, a healthy point is generated and the healthy point is determined to be the first color; If the target object content is outside the preset content range, a healthy point is generated and the healthy point is determined to be a second color.

10. The health history assessment method based on the history of changes in human body substance content according to claim 8, characterized in that: Generate health points based on the target content and / or target abnormality duration, including If the target object content is within the preset content range, a healthy point is generated and the healthy point is determined to be the first color; If the target object content is outside the preset content range, and the target object abnormality duration is less than or equal to the preset duration, a healthy point is generated, and the healthy point is determined to be the third color; If the target object content is outside the preset content range, and the target object abnormality duration is longer than the preset duration, a healthy point is generated, and the healthy point is determined to be the fourth color.

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