Water-soluble percutaneous tissue hyperplasia agent as well as preparation method and application thereof

Abstract

The invention discloses a water-soluble percutaneous tissue hyperplasia agent as well as a preparation method and application thereof, and belongs to the technical field of plant tissue hyperplasia. The proliferation agent is prepared by the following steps: dissolving sodium indoleacetate in a substrate solution to prepare a 400-600mg / L solution; the substrate liquid is prepared from the following raw materials in parts by weight: 7-11 parts of water, 1-5 parts of water-soluble wool fat and 1-3 parts of glycerol. The preparation method comprises the following steps: taking water, water-soluble wool fat, glycerol and sodium indoleacetate according to a formula proportion; the preparation method comprises the following steps: heating water to 45-60 DEG C, adding water-soluble wool fat, stirring and dissolving, adding glycerol, uniformly mixing, cooling to serve as a substrate solution, adding sodium indoleacetate, and uniformly stirring; the method for promoting the growth of the softwood by using the proliferative agent comprises the following steps: picking and stripping the softwood in the period of abundant water content of bark, coating the stripped wound surface with the prepared proliferative agent with the dosage of 0.05-0.25 ml / cm < 2 >, and then wrapping the wound surface with a degradable film. The proliferation agent can promote regeneration and differentiation of a cork cambium, delay dormancy, increase the cork growth amount, reduce sand inclusion formation and improve the cork quality uniformity.

Description

Technical Field

[0001] The invention belongs to the technical field of plant proliferation, and in particular relates to a water-soluble periderm proliferation agent and a preparation method and application thereof. Background Art

[0002] Quercus variabilis is an important cork source tree species in my country. Cork comes from the periodic division of the cork cambium of Quercus variabilis. The cork cambium forms the cork layer and cork tissue on the inner and outer sides respectively. The cork layer, cork cambium and cork tissue are collectively called periderm. The soft and elastic cork cells are commonly known as cork. The growth amount and growth uniformity of cork, such as the proportion of thin-walled and thick-walled cork cells, and the proportion of sand inclusions, i.e. stone cells, determine the quality, use and economic value of cork.

[0003] Many studies have shown that Quercus variabilis ( Quercus variabilis Bl.) cork and European cork oak ( Quercus suber Compared with cork, there are the following differences in periodic growth and cork quality: the growth ring width of domestic cork oak cork is narrower, about 200~400μm / year. After cork stripping, it takes 9~10 years, or even longer, to accumulate a certain amount of growth again to meet the needs of the second stripping. The proportion of thick-walled cork cells in the growth ring is relatively high, accounting for about 15~35%, and fluctuates greatly, which increases the overall density and hardness of the cork and makes the quality uneven. In addition, a large number of concentrated stone cell clusters are often seen in the growth ring, which are called sand inclusions. The density and hardness of stone cells are very different from those of cork tissue. The high proportion of sand inclusions exacerbates the unevenness of cork quality, which has an adverse effect on the high-value utilization of cork.

[0004] Based on this, the present invention aims to provide a water-soluble periderm proliferation agent to promote the regeneration of cork cambium of Quercus variabilis after cork stripping, increase the division frequency, delay the dormancy period, reduce the proportion of thick-walled cork cells and the formation of sand inclusions, comprehensively improve the growth amount and quality of Quercus variabilis cork, and enhance its economic value. Summary of the invention

[0005] The first object of the present invention is to provide a water-soluble peridermal tissue proliferation agent; the second object is to provide a method for preparing the water-soluble peridermal tissue proliferation agent; and the third object is to provide an application of the water-soluble peridermal tissue proliferation agent.

[0006] The first object of the present invention is achieved in that the water-soluble peridermal tissue proliferation agent is composed of sodium indoleacetic acid and a base liquid; the base liquid is prepared by mixing the following raw materials in parts by weight: 7 to 11 parts of water, 1 to 5 parts of water-soluble lanolin, and 1 to 3 parts of glycerol.

[0007] The second object of the present invention is achieved by including the steps of preparing and mixing the base liquid, specifically comprising: A. Preparation of base liquid: Heat the formulated water to 45-60°C, then add the formulated water-soluble lanolin, stir until dissolved, then add the formulated glycerol, mix and stir evenly to obtain base liquid a; B. Mixing: Add the formulated sodium indoleacetic acid into the base liquid a and stir evenly to obtain the target water-soluble wrinkled skin tissue hyperplasia agent.

[0008] The third object of the present invention is achieved by using the water-soluble periderm proliferation agent in promoting the growth of cork.

[0009] Compared with the prior art, the technical solution of the present invention has the following beneficial effects: 1. Use water-soluble sodium indoleacetic acid as the main agent for inducing proliferation, and avoid using alcohol-soluble indoleacetic acid, because highly permeable ethanol will inhibit wound healing, especially high concentrations of ethanol will cause rapid dehydration, death, and shrinkage of wound cells. During the cork regeneration process, the newly differentiated cork layer cells contain more tannin components, which can be decomposed by the glycerol in the base fluid, which is conducive to the continuous penetration of the main agent for inducing proliferation, acting on the mother cells in the differentiation period and reducing the barrier effect of the new tissue cells. In addition, the reduction of tannin components is also conducive to the stability of sodium indoleacetic acid and prolonging the effect of the proliferation agent.

[0010] 2. The drug-carrying base liquid composed of water-soluble lanolin, glycerol and water with a hanging performance can prolong the loading time of sodium indoleacetic acid on the peeling wound surface and avoid excessive drying and failure after application. The water content of the phloem of trees during peeling is ≥18%. By utilizing the difference in water content, not only can the hydrophobic cork tissue be easily separated, but the high water content of the residual tissue on the wound surface after peeling is also conducive to the slow penetration of the main agent in the base liquid into the wound tissue, continuously promoting the differentiation and growth of callus tissue, that is, the regenerated cork cambium and cork layer.

[0011] 3. After the wound surface is treated with a proliferative agent, it is coated with a film with a controllable degradation cycle to avoid the rapid photolysis of the proliferative agent sodium indoleacetic acid and reduce the formation of sand inclusions. The main materials of the biodegradable film are PLA and PBAT, which gradually degrade within 60 to 180 days, gradually improving the humidity and light environment around the healing wound surface and regenerated tissue, and avoiding the formation of an over-humid environment due to the condensation of water droplets in the film.

[0012] 4. After using the proliferative agent, the division frequency of the cork cambium of Quercus variabilis can be effectively increased, the wound surface is covered with proliferative tissue, the thickness of the cork layer proliferated monthly during the growth period can reach 100~120μm cell width, and the perimeter increase rate is ≥3% / month. Until the end of autumn, cork cells with large cavities and thin walls are still formed, which also shows that the proliferative agent has the effect of delaying the dormancy of the cambium, increasing the growth of cork, and improving the uniformity of cork quality.

[0013] 5. The growth promoter can not only be used to promote the growth of cork oak, but also has obvious effects on promoting wound healing and tissue proliferation for other tree injuries. It is simple to prepare, easy to use, and easy to promote and apply. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The wound surface of Quercus variabilis was obtained 2 months after the cork was harvested without any treatment. Figure 2 Under the same planting and nurturing conditions, cork was harvested according to the scheme described in Example 3, and the proliferation agent was applied immediately. After 2 months, the wound surface showed obvious tissue proliferation; Figure 3 Under the same planting and tending conditions, the cork layer grown according to the scheme described in Example 3 is composed entirely of thin-walled cork cells, with no stone cells. DETAILED DESCRIPTION

[0015] The present invention is further described below in conjunction with the embodiments, but the present invention is not limited in any way. Any changes or substitutions made based on the teachings of the present invention belong to the protection scope of the present invention.

[0016] The water-soluble peridermal tissue proliferation agent of the invention is composed of sodium indoleacetate and a base liquid; the base liquid is prepared by mixing the following raw materials in parts by weight: 7-11 parts of water, 1-5 parts of water-soluble lanolin and 1-3 parts of glycerol.

[0017] Specifically, sodium indoleacetate is dissolved in a base liquid to prepare a solution with a concentration of 400-600 mg / L; the base liquid is prepared by mixing the following raw materials in parts by weight: 7-11 parts of water, 1-5 parts of water-soluble lanolin, and 1-3 parts of glycerol.

[0018] The mass volume ratio of the sodium indoleacetic acid and the base solution is (450-550):1.

[0019] The weight ratio of raw materials of the base liquid is: 8-10 parts of water, 2-4 parts of water-soluble lanolin, and 2 parts of glycerol.

[0020] The method for preparing the water-soluble peridermal tissue proliferation agent of the present invention comprises the steps of preparing a base liquid and mixing, specifically comprising: A. Preparation of base liquid: Heat the formulated water to 45-60°C, then add the formulated water-soluble lanolin, stir until dissolved, then add the formulated glycerol, mix and stir evenly to obtain base liquid a; B. Mixing: Add the formulated sodium indoleacetic acid into the base liquid a and stir evenly to obtain the target water-soluble wrinkled skin tissue hyperplasia agent.

[0021] The mixing temperature in step B is controlled at 10~25°C.

[0022] The preparation method is specifically prepared by the following method: first, water, water-soluble lanolin, glycerol and sodium indoleacetic acid are measured according to the formula ratio and set aside; secondly, the water is heated to 45-60°C, the water-soluble lanolin is added to the water, and glycerol is added after stirring until dissolved, and the mixture is cooled to obtain a base liquid, and sodium indoleacetic acid is added to the base liquid and stirred again to obtain a base liquid.

[0023] The temperature of the base liquid is 10-25°C, preferably 15-20°C.

[0024] The application of the water-soluble periderm proliferation agent of the present invention is the application of the water-soluble periderm proliferation agent in promoting the growth of cork.

[0025] The water-soluble periderm proliferation agent for promoting cork growth specifically comprises the following steps: cork peeling is performed when the bark has rich water content, after peeling, the water-soluble periderm proliferation agent is applied to the peeled wound surface, and then the wound surface is wrapped with a degradable film.

[0026] The amount of the water-soluble periderm proliferation agent applied is 0.05-0.25 ml / cm 2 .

[0027] The bark having rich water content refers to the phloem water content being ≥ 18%.

[0028] The specific application operation is as follows: cork is peeled when the bark is rich in water content. After peeling, the prepared water-soluble periderm tissue proliferation agent is applied to the wound surface after peeling. The dosage is determined according to the wound area and the water content of the phloem, which is 0.05~0.25ml / cm 2 , and then wrap the wound with a degradable film.

[0029] The bark moisture-rich period refers to a period when the phloem moisture content is ≥18%.

[0030] In the bark moisture-rich period, if the phloem moisture content is 18-24%, the dosage of the water-soluble periderm proliferation agent is 0.05-0.15 ml / cm 2If the phloem moisture content is greater than 24%, the dosage of water-soluble periderm proliferation agent is 0.15~0.25ml / cm 2 .

[0031] The degradation period of the degradable film is 60 to 90 days.

[0032] If the phloem moisture content is 18-24%, the dosage of water-soluble periderm proliferation agent is 0.05-0.15 ml / cm 2 The degradation period of the degradable film is selected to be 70 to 90 days; if the phloem moisture content is ≥24%, the dosage of the water-soluble periderm proliferation agent is 0.15 to 0.25 ml / cm 2 The degradation period of the degradable film is selected to be 60 to 80 days.

[0033] The present invention is further described below with specific implementation cases: Example 1 The water-soluble peridermal tissue proliferation agent is prepared by the following method: according to the ratio of 10 parts of water, 2 parts of water-soluble lanolin, and 3 parts of glycerol, the base liquid raw material is measured, and sodium indoleacetic acid is weighed according to the ratio of 400 mg / L base liquid. The water is heated to 45-50°C, the water-soluble lanolin is added to the water, and the glycerol is added after stirring until dissolved. After mixing evenly, the temperature is cooled to 10°C as the base liquid, and the sodium indoleacetic acid is added to the base liquid and stirred evenly again.

[0034] Method for promoting cork growth with the growth agent: cork is peeled when the bark is rich in moisture, and the phloem moisture content is measured with a moisture meter. The average value of the 10 samples in the test site is 19.5%. Apply the prepared growth agent to the wound surface after peeling, with a dosage of 0.05ml / cm 2 , and then wrap the wound with a light-shielding film with a degradation period of 70 to 90 days.

[0035] Example 2 The water-soluble periderm tissue proliferation agent is prepared by the following method: according to the ratio of 10 parts of water, 2 parts of water-soluble lanolin and 2 parts of glycerol, the base liquid raw material is measured, and sodium indoleacetate is weighed according to the ratio of 450 mg / L base liquid. The water is heated to 45-50°C, the water-soluble lanolin is added to the water, and the glycerol is added after stirring until dissolved, and the mixture is evenly mixed and then cooled to 15°C as the base liquid, and the sodium indoleacetate is added to the base liquid and stirred again to be evenly mixed.

[0036] Method for promoting cork growth with the growth enhancer: cork is peeled when the bark is rich in moisture, and the phloem moisture content is measured with a moisture meter. The average value of the 10 samples in the test site is 19.9%. Apply the prepared growth enhancer on the peeled wound surface at a dosage of 0.08 ml / cm 2, and then wrap the wound with a light-shielding film with a degradation period of 70 to 90 days.

[0037] Example 3 The water-soluble peridermal tissue proliferation agent is prepared by the following method: according to the ratio of 9 parts of water, 3 parts of water-soluble lanolin and 1 part of glycerol, the base liquid raw material is measured, and sodium indoleacetic acid is weighed according to the ratio of 500 mg / L base liquid. The water is heated to 50-55°C, the water-soluble lanolin is added to the water, and the glycerol is added after stirring until dissolved, and the mixture is evenly mixed and then cooled to 20°C as the base liquid, and the sodium indoleacetic acid is added to the base liquid and stirred again to be evenly mixed.

[0038] Method for promoting cork growth with the growth agent: cork is peeled when the bark is rich in moisture, and the phloem moisture content is measured with a moisture meter. The average value of the 10 samples in the test site is 23.1%. Apply the prepared growth agent to the wound surface after peeling, with a dosage of 0.15ml / cm 2 , and then wrap the wound with a light-shielding film with a degradation period of 70 to 90 days.

[0039] Example 4 The water-soluble periderm tissue proliferation agent is prepared by the following method: according to the ratio of 7 parts of water, 5 parts of water-soluble lanolin and 1 part of glycerol, the base liquid raw material is measured, and sodium indoleacetic acid is weighed according to the ratio of 600 mg / L base liquid. The water is heated to 55-60°C, the water-soluble lanolin is added to the water, and the glycerol is added after stirring until dissolved. After mixing evenly, the temperature is cooled to 25°C as the base liquid, and the sodium indoleacetic acid is added to the base liquid and stirred evenly again.

[0040] Method for promoting cork growth with the growth agent: cork is peeled when the bark is rich in moisture, and the phloem moisture content is measured with a moisture meter. The average value of the 10 samples in the test site is 31.4%. Apply the prepared growth agent to the wound surface after peeling, with a dosage of 0.25ml / cm 2 , and then wrap the wound with a light-shielding film with a degradation period of 60 to 80 days.

[0041] Example 5 The water-soluble peridermal tissue proliferation agent is prepared by the following method: according to the ratio of 8 parts of water, 4 parts of water-soluble lanolin and 1 part of glycerol, the base liquid raw material is measured, and sodium indoleacetic acid is weighed according to the ratio of 550 mg / L base liquid. The water is heated to 55-60°C, the water-soluble lanolin is added to the water, and the glycerol is added after stirring until dissolved, and the mixture is evenly mixed and then cooled to 20°C as the base liquid, and the sodium indoleacetic acid is added to the base liquid and stirred again to be evenly mixed.

[0042] Method for promoting cork growth with the growth agent: cork is peeled when the bark is rich in moisture, and the phloem moisture content is measured with a moisture meter. The average value of the 10 samples in the test site is 24.8%. The prepared growth agent is applied to the wound surface after peeling, with a dosage of 0.18ml / cm 2 , and then wrap the wound with a light-shielding film with a degradation period of 60 to 80 days.

Claims

1. A water-soluble periderm proliferation agent, characterized in that: The water-soluble peridermal tissue proliferation agent is composed of sodium indoleacetate and a base liquid; the base liquid is prepared by mixing the following raw materials in parts by weight: 7-11 parts of water, 1-5 parts of water-soluble lanolin, and 1-3 parts of glycerol.

2. The water-soluble periderm proliferation agent according to claim 1, characterized in that The mass volume ratio of the sodium indoleacetic acid and the base solution is (450-550):

1.

3. The water-soluble periderm proliferation agent according to claim 1, characterized in that The weight ratio of raw materials of the base liquid is: 8-10 parts of water, 2-4 parts of water-soluble lanolin, and 2 parts of glycerol.

4. A method for preparing the water-soluble periderm proliferation agent according to any one of claims 1 to 3, characterized in that: The method includes the steps of preparing the base liquid and mixing the base liquid, specifically including: A. Preparation of base liquid: Heat the formulated water to 45-60°C, then add the formulated water-soluble lanolin, stir until dissolved, then add the formulated glycerol, mix and stir evenly to obtain base liquid a; B. Mixing: Add the formulated sodium indoleacetate into the base solution a and stir evenly to obtain the target water-soluble peridermal tissue proliferation agent.

5. The preparation method according to claim 4, characterized in that: The mixing temperature in step B is controlled at 10~25°C.

6. A use of the water-soluble periderm proliferation agent according to any one of claims 1 to 3, characterized in that: The water-soluble periderm proliferation agent is used in promoting the growth of cork.

7. The use according to claim 6, characterized in that: The water-soluble periderm proliferation agent promotes the growth of cork, which specifically comprises the following steps: cork peeling is performed during the period when the bark has rich water content, after peeling, the water-soluble periderm proliferation agent is applied to the peeled wound surface, and then the wound surface is wrapped with a degradable film.

8. The use according to claim 7, characterized in that: The amount of the water-soluble periderm proliferation agent applied is 0.05-0.25 ml / cm 2 .

9. The use according to claim 7, characterized in that: The bark having rich water content refers to the phloem water content being ≥ 18%.

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