Leukocyte-poor PRP extraction method based on leukocyte-poor PRP extraction device

Abstract

The invention discloses a leukocyte-poor PRP extraction method based on a leukocyte-poor PRP extraction device. The device comprises a piston and a base, and one end of the piston is fixedly connected to the base; one end of the second accommodating cavity is embedded in the first accommodating cavity, the other end of the second accommodating cavity is embedded in the third accommodating cavity, the base covers an opening of the third accommodating cavity through threaded connection, the volume of a collected blood sample is defined as Lml, the minimum volume of the third accommodating cavity reaching the top limit position is 0.30-0.35 * L, and the volume of the second accommodating cavity is 0.2-0.3 * L; the separation assembly comprises a screw rod which sequentially penetrates through the base and the piston and extends to the third containing cavity, and a plug and a knob which are connected to the two ends of the screw rod; the plug head is used for blocking the second containing cavity, and the plug head can be driven by the screw to move in the length direction of the second containing cavity so as to communicate or close the second containing cavity and the third containing cavity; the pipe plug extends towards the first containing cavity and is provided with a protrusion.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a leukocyte-poor PRP extraction method based on a leukocyte-poor PRP extraction device. Background Art

[0002] Platelet-rich plasma (PRP) has been widely used in the medical field, especially in regenerative medicine, trauma treatment and aesthetic medicine. PRP helps accelerate tissue repair and regeneration through its high concentration of platelets and growth factors. However, during the traditional PRP extraction process, a high number of white blood cells are usually extracted at the same time, which may cause an inflammatory response and be detrimental to the treatment effect. For example:

[0003] Arthritis: LP-PRP helps reduce inflammation and pain and promotes cartilage repair. Suitable for knee arthritis, hip arthritis, etc.

[0004] Tendinopathy: Used to treat chronic tendinopathy such as tennis elbow and Achilles tendinitis because its low white blood cell count reduces the inflammatory response.

[0005] Cosmetic Surgery: Used in skin regeneration and anti-aging treatments such as facial skin rejuvenation, its low-inflammatory properties help reduce swelling and pain.

[0006] Soft tissue injuries: including muscle tears, ligament injuries, etc. LP-PRP helps accelerate the healing process and reduce inflammatory response.

[0007] Therefore, the demand for leukocyte-poor platelet plasma (LR-PRP) is increasing, as it can significantly reduce the content of leukocytes while providing high concentrations of platelets, thereby reducing the inflammatory response and improving the therapeutic effect.

[0008] Most of the PRP extraction devices on the market can only perform ordinary PRP extraction and cannot effectively remove white blood cells. Existing technical means mainly rely on multiple centrifugation and manual operation to separate white blood cells, which is not only complicated and time-consuming, but also easy to introduce contamination, reducing the extraction efficiency and purity of PRP. In addition, the existing devices do not handle blood samples gently during operation, which may lead to reduced platelet activity and affect the final treatment effect. Summary of the invention

[0009] In view of the problems existing in the above-mentioned prior art, the present invention provides a leukocyte-poor PRP extraction method based on a leukocyte-poor PRP extraction device, which can effectively solve the problems existing in the above-mentioned prior art.

[0010] The technical solution of the present invention is:

[0011] According to one aspect of the present invention, the extraction device comprises: a centrifuge tube and a tube plug that are interlocked, wherein the centrifuge tube comprises, from top to bottom, a first accommodating chamber, a second accommodating chamber, and a third accommodating chamber that are sequentially connected, and the inner diameter of the second accommodating chamber is smaller than the inner diameter of the third accommodating chamber;

[0012] It also includes a piston and a base, one end of the piston is fixedly connected to the base; the other end is embedded in the third accommodating chamber, the base is threadedly connected to the opening of the third accommodating chamber, the volume of the collected blood sample is defined as Lml, the minimum volume of the third accommodating chamber at the top limit position is 0.30-0.35*L, and the volume of the second accommodating chamber is 0.2-0.3*L;

[0013] A separation assembly, comprising a screw rod sequentially penetrating the base and the piston and extending to the third accommodating chamber, and a plug and a knob connected to both ends of the screw rod; the plug rod is used to block the second accommodating chamber, and the plug rod can be driven by the screw rod to move along the length direction of the second accommodating chamber to connect or close the second accommodating chamber and the third accommodating chamber;

[0014] The pipe plug is provided with a protrusion extending toward the first accommodating cavity;

[0015] The extraction method comprises the following steps:

[0016] S1, the extraction device is arranged vertically in the forward direction, the base is rotated toward the third accommodating chamber to the top limit position, and then the plug is moved away from the second accommodating chamber to connect the first accommodating chamber, the second accommodating chamber and the third accommodating chamber;

[0017] S2, injecting the collected blood sample into a centrifuge tube;

[0018] S3, starting the centrifuge, controlling the centrifugal force and time of the centrifuge to perform the first centrifugation, so that the blood sample is separated into a red blood cell layer, a white blood cell layer and a plasma layer from bottom to top;

[0019] S4, slowly rotating the base to drive the piston to move downward, so that the red blood cell layer and part of the white blood cells in the second accommodation chamber slowly flow into the third accommodation chamber, so that the boundary line between the white blood cell layer and the plasma layer is located at the bottom of the second accommodation chamber;

[0020] S5, slowly turning the knob to make the screw drive the plug to move toward the second accommodating chamber, so that the plug is blocked in the second accommodating chamber, thereby separating the white blood cell layer from the plasma layer;

[0021] S6, turning the extraction device 180 degrees, starting the centrifuge, and controlling the centrifugal force and time of the centrifuge to perform a second centrifugation, so that the white blood cells are concentrated at the bottom of the periphery of the protrusion, and the white blood cell-poor PRP is deposited on the white blood cell layer;

[0022] S7, inserting the injection device from the bottom of the tube plug into the protrusion and passing through the protrusion, thereby bypassing the white blood cells around the protrusion and collecting the white blood cell-poor PRP.

[0023] Further, in step S3, the step of controlling the centrifugal force and time of the centrifuge to perform the first centrifugation so that the blood sample is separated from bottom to top into a red blood cell layer, a white blood cell layer and a plasma layer specifically includes:

[0024] The centrifugal force of the centrifuge is controlled to be 380-420 g, and the centrifugal time is 3-10 minutes for the first centrifugation, so that the blood sample is separated into a red blood cell layer, a white blood cell layer and a plasma layer from bottom to top.

[0025] Furthermore, in step S6, the step of turning the extraction device 180°, starting the centrifuge, and controlling the centrifugal force and time of the centrifuge to perform a second centrifugation to concentrate the platelets and part of the white blood cells in the bottom layer specifically includes:

[0026] The centrifugal force of the centrifuge is controlled to be 950-1050 g, and the centrifugal time is 5-15 minutes for a second centrifugation to concentrate the platelets and part of the leukocytes in the bottom layer.

[0027] Further, in step S2, the step of injecting the collected blood sample into the centrifuge tube specifically includes:

[0028] Insert the injection device from the top of the tube stopper and inject the collected blood sample into the centrifuge tube.

[0029] Further, in step S3, when the boundary between the white blood cell layer and the plasma layer is located in the third containing chamber, a preliminary judgment is made as to a blood problem, and the process ends.

[0030] Furthermore, L is 5 to 100 ml, and the minimum volume of the third accommodating chamber when it reaches the top limit position is 0.33 to 0.34*L.

[0031] Furthermore, the inner diameter of the second accommodating cavity is 1 to 1.8 cm.

[0032] Further, in step S5, when the knob is slowly turned to make the screw drive the plug to move toward the second accommodating chamber, the boundary line between the white blood cell layer and the plasma layer rises significantly, the base is slowly rotated to drive the piston to move downward, so that the boundary line between the white blood cell layer and the plasma layer is always controlled to be located at the bottom of the second accommodating chamber.

[0033] Further, in step S3, when the boundary between the white blood cell layer and the plasma layer is located in the first containing cavity, a preliminary judgment is made as to a blood problem, and the process ends.

[0034] Furthermore, the height of the protrusion is 3 to 6 mm. In step S7, an injection device is inserted into the top of the protrusion to collect platelets and plasma.

[0035] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art:

[0036] This solution provides an efficient and easy-to-operate leukocyte-poor platelet plasma (P-LR PRP) extraction device. This device can effectively separate and extract high-concentration platelets in a simple operation, while significantly reducing the content of white blood cells, simplifying the operation steps, reducing the risk of contamination, and improving the extraction efficiency and platelet activity, thereby better meeting clinical needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0038] Figure 1 This is a schematic diagram of the vertical cross-sectional structure of the extraction device in the present invention;

[0039] Figure 2 It is a schematic cross-sectional structure diagram of the plug plugging the second accommodating cavity in the present invention;

[0040] Figure 3 This is a structural schematic diagram of the present invention, in which the injection device is inserted into the first accommodating cavity when the extraction device is rotated 180° and the extraction device is reversed to be vertical;

[0041] In the figure: centrifuge tube-1, first accommodating chamber-11, third accommodating chamber-12, second accommodating chamber-13, tube plug-2, inclined surface-21, protrusion-22, support frame-3, piston-4, limiting groove-41, base-5, connecting rod-51, limiting flange-52, separation component-6, plug-61, screw-62, knob-63, injection device-A. DETAILED DESCRIPTION

[0042] The present invention will be further described in detail below in conjunction with the accompanying drawings and examples. It is particularly noted that the following examples are only used to illustrate the present invention, but are not intended to limit the scope of the present invention. Similarly, the following examples are only partial embodiments of the present invention rather than all embodiments, and all other embodiments obtained by those of ordinary skill in the art without creative work are within the scope of protection of the present invention.

[0043] like Figures 1 to 3 As shown, the present scheme provides a leukocyte-poor PRP extraction method based on a leukocyte-poor PRP extraction device.

[0044] See also Figure 1 and Figure 2 The extraction device includes: a centrifuge tube 1 and a tube plug 2 that are interlocked, and in this embodiment, a support frame 3 is also included. The support frame 3 is fixedly sleeved on the outside of the tube plug 2, and the support frame 3 is threadedly connected to the first accommodating cavity. The tube plug 2 is made of silicone; it is convenient for the injection device A to be inserted into the tube plug 2 to collect platelets and plasma.

[0045] See also Figure 1 and Figure 2 , further comprising a base 5 and a piston 4, the piston 4 being embedded in the third accommodating chamber 12 so as to be movable up and down, one end of the piston 4 being fixedly connected to the base 5, and the base 5 being covered by a threaded connection at the opening of the third accommodating chamber 12. The initial state of the base 5 is to rotate to the limit position toward the third accommodating chamber 12. Specifically, the internal thread of the base 5 is matched and connected with the external thread of the third accommodating chamber 12. Preferably, a connecting rod 51 is provided at one end of the base 5 extending toward the third accommodating chamber 12, the connecting rod 51 is provided with a limiting flange 52, and the piston 4 is correspondingly provided with a limiting groove 41, and the limiting flange 52 is engaged with the limiting groove 41.

[0046] See also Figure 1 and Figure 2 , the centrifuge tube 1 includes from top to bottom: a first accommodating chamber 11, a second accommodating chamber 13 and a third accommodating chamber 12 which are connected in sequence, and the inner diameter of the second accommodating chamber 13 is smaller than the inner diameter of the third accommodating chamber 12. The volume of the collected blood sample is defined as Lml, wherein L can be 5 to 100ml, in which many embodiments, L is 10 to 30ml, and in this embodiment, L is about 20ml. Since the red blood cell content in normal human whole blood is between 35% and 55%. The white blood cell content in normal human whole blood is between 0.5% and 1.5%. After the whole blood is centrifuged, obvious stratification will be formed according to the different densities of each component. Among them, the white blood cell layer is located between the plasma layer and the red blood cell layer, presenting a relatively thin white layered structure. Usually, in order to make the boundary between the white blood cell layer and the plasma located in the second accommodation chamber 13 in the subsequent separation process, preferably, the minimum volume of the third accommodation chamber 12 at the top limit position should be 0.30-0.35*L, more preferably, the minimum volume of the third accommodation chamber 12 at the top limit position should be 0.33-0.34*L. In this embodiment, the minimum volume of the third accommodation chamber 12 at the top limit position is about 6.8ml. The maximum volume of the third accommodation chamber 12 at the bottom limit position is not limited, and preferably can be about 0.5*L. In this embodiment, the maximum volume of the third accommodation chamber 12 at the bottom limit position is about 10ml.

[0047] The volume of the second accommodating chamber 13 is preferably 0.2 to 0.3*L. The advantage is that when about 35% of the red blood cells fill the third accommodating chamber 12 at the top limit position during the first centrifugal separation, the remaining red blood cells and white blood cell layer will be located in the second accommodating chamber 13, so that the boundary between white blood cells and plasma is also located in the second accommodating chamber 13, which is convenient for the subsequent separation of the red blood cell layer and the white blood cell layer from the plasma. In this embodiment, the volume of the second accommodating chamber 13 is preferably 4ml to 6ml, and preferably, the volume of the second accommodating chamber 13 is preferably 5ml.

[0048] In this embodiment, further, in order to make the boundary between white blood cells and plasma more obvious, under the premise that the volume of the second accommodating chamber 13 remains unchanged, the inner diameter of the second accommodating chamber 13 can be further reduced to increase the height of the second accommodating chamber 13. However, as the inner diameter decreases, the stratification of white blood cells and plasma will not be complete (as the inner diameter decreases, the centrifugal force also decreases, F=mω2r, where F represents centrifugal force, m represents mass, ω represents angular velocity, and r represents radius). Experiments have shown that when the inner diameter of the second accommodating chamber 13 is less than 1 cm, it is difficult to effectively separate white blood cells and plasma when the centrifugal force of the first centrifugation is 400g (g is the acceleration of gravity). Taking the volume of the second accommodating chamber 13 as 5ml for example (V=π*R 2 *h, V represents volume, R represents radius, and h represents height), preferably, the inner diameter of the second accommodating chamber 13 is 1 cm to 1.8 cm, and the corresponding height of the second accommodating chamber 13 is 6.37 to 1.97 cm; more preferably, the inner diameter of the second accommodating chamber 13 is 1.2 cm to 1.6 cm, and the corresponding height of the second accommodating chamber 13 is 4.42 to 2.49 cm.

[0049] See also Figures 1 to 3 , also includes a separation component 6 penetrated in the third accommodating chamber 12, the separation component 6 moves along the length direction of the second accommodating chamber 13 to connect or close the second accommodating chamber 13 and the third accommodating chamber 12; preferably, the separation component 6 includes a plug 61, a screw 62 and a knob 63, the screw 62 penetrates the base 5 and the third accommodating chamber 12, and the screw 62 is threadedly connected to the base 5; the plug 61 can be driven by the screw 62 to move along the length direction of the second accommodating chamber 13 to connect or close the second accommodating chamber 13 and the third accommodating chamber 12; one end of the screw 62 is fixed with a plug 61 for blocking the second accommodating chamber 13, and the other end is fixed with a knob 63.

[0050] See also Figure 1 and Figure 2One end of the tube plug 2 is provided with an annular inclined surface 21 extending toward the inner circumference of the first accommodating chamber 11. Therefore, when the extraction device is reversed and vertical, the space from the junction of the first accommodating chamber 11 and the tube plug 2 to the upper end surface of the first accommodating chamber 11 is wide at the top and narrow at the bottom, which is convenient for observing the collection of platelets and plasma.

[0051] See also Figures 1 to 3 , the tube plug 2 is provided with a protrusion 22 extending toward the first accommodating chamber 11. The height of the protrusion 22 is 3 to 6 mm, and preferably, the height of the protrusion 22 is 4 mm. To ensure that the white blood cells after the second centrifugation can be concentrated around the protrusion 22, the injection device A is inserted into the top of the protrusion 22, and the white blood cells can be avoided when collecting platelets and plasma. The raised surface of the protrusion 22 is arc-shaped. When the second centrifugation is performed, the extraction device is turned 180°. After centrifugation, the white blood cells will be concentrated around the protrusion 22. At this time, the injection device A is inserted into the arc-shaped top of the protrusion 22 to collect the platelets and plasma.

[0052] The extraction method comprises the following steps:

[0053] S1, the extraction device is set vertically in the forward direction, the base 5 is rotated toward the third accommodating chamber 12 to the top limit position, and then the plug 61 is moved away from the second accommodating chamber 13, so that the first accommodating chamber 11, the second accommodating chamber 13 and the third accommodating chamber 12 are connected;

[0054] S2, injecting the collected blood sample into the centrifuge tube 1; the step of injecting the collected blood sample into the centrifuge tube 1 specifically includes: inserting the injection device A from the top of the tube plug 2 and injecting the collected blood sample into the centrifuge tube 1.

[0055] S3, start the centrifuge, control the centrifugal force and time of the centrifuge to perform the first centrifugation, so that the blood sample is separated from the bottom to the top into a red blood cell layer, a white blood cell layer and a plasma layer; the step of controlling the centrifugal force and time of the centrifuge to perform the first centrifugation, so that the blood sample is separated from the bottom to the top into a red blood cell layer, a white blood cell layer and a plasma layer specifically includes: controlling the centrifugal force of the centrifuge to be 380-420g, the centrifugal time to be 3-10 minutes for the first centrifugation, so that the blood sample is separated from the bottom to the top into a red blood cell layer, a white blood cell layer and a plasma layer. Preferably, the centrifugal force of the centrifuge is controlled to be 400g, and the centrifugal time is 5 minutes.

[0056] When the boundary between the white blood cell layer and the plasma layer is located in the third accommodating chamber 12, the blood problem is initially determined and the procedure ends. When the boundary between the red blood cell layer and the white blood cell layer is located in the third accommodating chamber 12, it indicates that the content of red blood cells and white blood cells in the whole blood is between 35% and 57%.

[0057] When the boundary between the white blood cell layer and the plasma layer is located in the first accommodating chamber 11, a blood problem is preliminarily determined and the procedure ends. When the boundary between the red blood cell layer and the white blood cell layer is located in the first accommodating chamber 11, it means that the content of red blood cells and white blood cells in the whole blood exceeds 57%.

[0058] S4, slowly rotating the base 5 to drive the piston 4 to move downward, so that the red blood cell layer and part of the white blood cells in the second accommodating chamber 13 slowly flow into the third accommodating chamber 12, so that the boundary between the white blood cell layer and the plasma layer is located at the bottom of the second accommodating chamber 13;

[0059] S5, slowly turn the knob 63 to make the screw 62 drive the plug 61 to move toward the second accommodating chamber 13, so that the plug 61 is blocked in the second accommodating chamber 13, thereby separating the white blood cell layer from the plasma layer; when the knob 63 is slowly turned to make the screw 62 drive the plug 61 to move toward the second accommodating chamber 13, when the boundary line between the white blood cell layer and the plasma layer rises significantly, the base 5 is slowly rotated to drive the piston 4 to move downward, so that the boundary line between the white blood cell layer and the plasma layer is always located at the bottom of the second accommodating chamber 13.

[0060] S6, flip the extraction device 180°, start the centrifuge, control the centrifugal force and time of the centrifuge to perform a second centrifugation, so that the white blood cells are concentrated at the bottom around the protrusion 22, and the white blood cell-poor PRP is deposited on the white blood cell layer; flip the extraction device 180°, start the centrifuge, control the centrifugal force and time of the centrifuge to perform a second centrifugation, so that the platelets and part of the white blood cells are concentrated in the bottom layer. The step specifically includes: controlling the centrifugal force of the centrifuge to 950-1050g, the centrifugal time to 5-15 minutes for the second centrifugation, so that the platelets and part of the white blood cells are concentrated in the bottom layer. Preferably, the centrifugal force of the centrifuge is controlled to 1000g, and the centrifugal time is 10 minutes.

[0061] S7, insert the injection device A from the bottom of the tube plug 2 into the protrusion 22 and pass through the protrusion 22, thereby bypassing the white blood cells around the protrusion 22 and collecting the white blood cell-poor PRP. Specifically, the injection device A is inserted into the top of the protrusion 22 to collect platelets and plasma.

[0062] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device, characterized in that: The extraction device comprises: a centrifuge tube (1) and a tube plug (2) which are interlocked, wherein the centrifuge tube (1) comprises, from top to bottom, a first accommodating chamber (11), a second accommodating chamber (13) and a third accommodating chamber (12) which are sequentially connected, and the inner diameter of the second accommodating chamber (13) is smaller than the inner diameter of the third accommodating chamber (12); It also includes a piston (4) and a base (5), one end of the piston (4) is fixedly connected to the base (5); the other end is embedded in the third accommodating chamber (12), the base (5) is connected to the opening of the third accommodating chamber (12) through a threaded cover, the volume of the collected blood sample is defined as Lml, the minimum volume of the third accommodating chamber (12) at the top limit position is (0.30-0.35)*L, and the volume of the second accommodating chamber (13) is (0.2-0.3)*L; A separation assembly (6), comprising a screw (62) which sequentially passes through the base (5) and the piston (4) and extends to the third accommodating chamber (12), and a plug (61) and a knob (63) connected to both ends of the screw (62); the plug (61) is used to block the second accommodating chamber (13); the plug (61) can be driven by the screw (62) to move along the length direction of the second accommodating chamber (13) to connect or close the second accommodating chamber (13) and the third accommodating chamber (12); The pipe plug (2) is provided with a protrusion (22) extending toward the first accommodating cavity (11); The extraction method comprises the following steps: S1, the extraction device is arranged vertically in the forward direction, the base (5) is rotated toward the third accommodating chamber (12) to the top limit position, and then the plug (61) is moved away from the second accommodating chamber (13), so that the first accommodating chamber (11), the second accommodating chamber (13) and the third accommodating chamber (12) are connected; S2, injecting the collected blood sample into a centrifuge tube (1); S3, starting the centrifuge, controlling the centrifugal force and time of the centrifuge to perform the first centrifugation, so that the blood sample is separated into a red blood cell layer, a white blood cell layer and a plasma layer from bottom to top; S4, slowly rotating the base (5) to drive the piston (4) to move downward, so that the red blood cell layer and part of the white blood cells in the second accommodating chamber (13) slowly flow into the third accommodating chamber (12), so that the boundary between the white blood cell layer and the plasma layer is located at the bottom of the second accommodating chamber (13); S5, slowly turning the knob (63) so that the screw (62) drives the plug (61) to move toward the second accommodating chamber (13), so that the plug (61) is blocked in the second accommodating chamber (13), thereby separating the white blood cell layer from the plasma layer; S6, turning the extraction device 180 degrees, starting the centrifuge, controlling the centrifugal force and time of the centrifuge to perform a second centrifugation, so that the white blood cells are concentrated at the bottom of the periphery of the protrusion (22), and the white blood cell-poor PRP is deposited on the white blood cell layer; S7, insert the injection device (A) from the bottom of the tube plug (2) into the protrusion (22) and pass through the protrusion (22), thereby bypassing the white blood cells around the protrusion (22) and collecting the white blood cell-poor PRP.

2. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: In step S3, the step of controlling the centrifugal force and time of the centrifuge to perform the first centrifugation so that the blood sample is separated from bottom to top into a red blood cell layer, a white blood cell layer and a plasma layer specifically includes: The centrifugal force of the centrifuge is controlled to be 380-420 g, and the centrifugal time is 3-10 minutes for the first centrifugation, so that the blood sample is separated into a red blood cell layer, a white blood cell layer and a plasma layer from bottom to top.

3. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: In step S6, the extraction device is turned over 180 degrees, the centrifuge is started, and the centrifugal force and time of the centrifuge are controlled to perform a second centrifugation to concentrate the platelets and part of the white blood cells in the bottom layer. Specifically, the steps include: The centrifugal force of the centrifuge is controlled to be 950-1050 g, and the centrifugal time is 5-15 minutes for a second centrifugation to concentrate the platelets and part of the leukocytes in the bottom layer.

4. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: In step S2, the step of injecting the collected blood sample into the centrifuge tube (1) specifically includes: Insert the injection device (A) from the top of the tube stopper (2) and inject the collected blood sample into the centrifuge tube (1).

5. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: In step S3, when the boundary between the white blood cell layer and the plasma layer is located in the third containing chamber (12), a preliminary judgment is made as to the blood problem and the process ends.

6. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: L is 5 to 100 ml, and the minimum volume of the third accommodating chamber (12) when it reaches the top limit position is (0.33 to 0.34)*L.

7. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 6, characterized in that: The inner diameter of the second accommodating chamber (13) is 1 to 1.8 cm.

8. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: In step S5, when the knob (63) is slowly rotated to cause the screw (62) to drive the plug (61) to move toward the second accommodating chamber (13), when the boundary between the white blood cell layer and the plasma layer rises significantly, the base (5) is slowly rotated to drive the piston (4) to move downward, so that the boundary between the white blood cell layer and the plasma layer is always located at the bottom of the second accommodating chamber (13).

9. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: In step S3, when the boundary between the white blood cell layer and the plasma layer is located in the first containing chamber (11), a preliminary judgment is made as to the blood problem and the process ends.

10. A method for extracting leukocyte-poor PRP based on a leukocyte-poor PRP extraction device as claimed in claim 1, characterized in that: The height of the protrusion (22) is 3 to 6 mm. In step S7, the injection device (A) is inserted into the top of the protrusion (22) to collect platelets and plasma.

Images